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Pathomorphological changes in the larvae cells of blood-suckıng mosquitoes (Aedes caspius Pallas, 1771) affected by parasitizing microsporidium Amblyospora (=Thelohania) opacita Kudo, 1922 blood-suckıng蚊(Aedes caspius Pallas, 1771)受Amblyospora (= theelohania) opacita Kudo, 1922)侵染后幼虫细胞病理形态学的变化
Pub Date : 2020-01-01 DOI: 10.26565/2075-5457-2020-35-11
Microsporidia are highly specialized obligate intracellular parasites. They affect various tissues of most animal groups. In Azerbaijan, 29 species and forms of microsporidia were recorded. Of these, 10 species (Amblyospora minuta, Pleistophora obesa, Thelohania opacita, Th. opacita caspius, Th. vexans, Stempellia captshagaica, St. magna, Nosema caspius, Nosema sp., Culicosporella sp.) were found in four species of blood-sucking mosquitos (Culix pipiens pipiens, Aedes vexans, A. caspius, Culex theileri). The collected larvae were identified using the key of Gutsevich et al. (1970). In the laboratory, the mosquito larvae were examined against a dark background under the microscope MBS-9 to distinguish individuals infected with microsporidia. Smears were stained with azure-eosin. Histological slices were prepared according to the Volkova and Yeletskiy method (1971); pathological changes in host tissues were identified using the electron microscope JEM 1400. In the course of our research conducted in 2017–2018 on the Absheron peninsula (Azerbaijan), the life stages of the microsporidium Amblyospora (=Thelohania) opacita Kudo, 1922 were found in the larvae of Aedes caspius Pallas, 1771. Examination of the infected host cell ultrastructure revealed the following changes: rough endoplasmic reticulum and mitochondria concentration around the parasite, an increase of cytoplasm volume, initiation of cell hypertrophy, disappearance of fat, protein granules and rough endoplasmic reticulum at later development stages, a decrease in the number of ribosomes in the cytoplasm and their simultaneous increase around the periphery of the nucleus, mitochondria degradation. These changes cause a delay in the larva development. Microsporidiosis affects the whole mosquito life cycle. The effect of microsporidia on the host organism manifests itself in the delayed larvae development and, in some cases, their early death. First of all, the lipid granules disappear supposedly because of the intensification of the host's aerobic metabolism to compensate for the energy loss caused by the developing parasites.
微孢子虫是高度特化的专性细胞内寄生虫。它们影响大多数动物群体的各种组织。在阿塞拜疆,记录了29种和形式的小孢子虫。其中,10种(Amblyospora minuta, Pleistophora obesa, thelelohania opacita, Th。不透明的caspius。在4种吸血蚊(库蚊、刺刺伊蚊、刺刺伊蚊、库氏小孢子虫、库氏小孢子虫、刺刺伊蚊)中检出刺刺蚊、刺刺天蛾、刺刺天蛾、库氏库蚊。收集的幼虫采用Gutsevich et al.(1970)的鉴定方法。在实验室中,在MBS-9显微镜下对暗色背景下的蚊子幼虫进行检查,以区分感染微孢子虫的个体。涂片用蓝-伊红染色。根据Volkova和Yeletskiy方法(1971)制备组织切片;用JEM 1400电镜观察宿主组织的病理变化。2017-2018年在阿塞拜疆阿布歇隆半岛进行的研究中,在1771年的caspius Pallas伊蚊幼虫中发现了Amblyospora (=Thelohania) opacita Kudo, 1922年的生命阶段。寄主细胞超微结构检查显示:寄主周围粗大的内质网和线粒体浓度,细胞质体积增大,细胞开始肥大,发育后期脂肪、蛋白颗粒和粗大的内质网消失,细胞质中核糖体数量减少,核周周围核糖体数量同时增加,线粒体降解。这些变化会延迟幼虫的发育。微孢子虫病影响蚊子的整个生命周期。微孢子虫对宿主生物的影响表现在幼虫发育迟缓,在某些情况下,它们的早期死亡。首先,脂质颗粒的消失可能是因为寄主有氧代谢的加强,以补偿寄生物发育所造成的能量损失。
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引用次数: 0
POLITICAL CORRUPTION AS A SYSTEM OF ILLEGAL ACTIONS: VARIABILITY OF ASPECTS 政治腐败作为一种违法行为体系:各方面的可变性
Pub Date : 2020-01-01 DOI: 10.26565/2220-8089-2020-38-02
Theoretical and methodological bases of political corruption as a systemic problem are considered. Political corruption is analyzed as a complex political, socio-economic, legal phenomenon, its main features are studied, the main approaches to the interpretation of the concept of political corruption are systematized, the causes of corrupt economic relations are clarified. Emphasis is placed on the fact that political corruption is a specific element of the socio-political life of society and the state as a whole. It reduces the ability of public authorities to realistically and effectively solve various objectively arising social problems. The phenomenon of political corruption has a socio-global nature, mediated by a set of economic, political, socio-cultural factors of society. The conceptual consideration of this aspect of political corruption is based on the assertion that political corruption is a multilateral but holistic phenomenon. In other words, revealing political corruption in this aspect, it is necessary not only to identify its essential features in the framework of political, legal, socio-economic and socio-cultural approaches, but also to trace the relationship between them, to understand their combination in a single phenomenon. It turns out that the main mechanism of control of politicians, accountability to their voters are always civil society institutions, the absence of which, as well as the dependence of the legislative and judicial branches of government inevitably lead to political corruption, which in turn completes the cycle of political corruption. generates the production of such actions of the authorities.
政治腐败作为一个系统问题的理论和方法基础进行了考虑。分析了政治腐败作为一种复杂的政治、社会经济、法律现象,研究了政治腐败的主要特征,对政治腐败概念解释的主要途径进行了系统梳理,澄清了腐败经济关系的成因。重点放在政治腐败是社会和国家作为一个整体的社会政治生活的一个特定因素。它降低了公共当局现实有效地解决各种客观出现的社会问题的能力。政治腐败现象具有社会全球性质,由一系列社会经济、政治和社会文化因素介导。对政治腐败这一方面的概念考虑是基于政治腐败是一种多边但整体的现象的断言。换句话说,在这方面揭示政治腐败,不仅需要在政治、法律、社会经济和社会文化途径的框架中识别其本质特征,而且需要追踪它们之间的关系,将它们结合在一个单一的现象中。事实证明,政治家的主要控制机制、对选民的责任始终是公民社会机构,公民社会机构的缺失以及政府立法和司法部门的依赖不可避免地导致政治腐败,从而完成政治腐败的循环。产生产生这样的行为的当局。
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引用次数: 0
The effect of nitrogen monoxide donors on the indexes of cadmium-induced oxidative stress in different rat tissues 一氧化氮供体对镉诱导大鼠不同组织氧化应激指标的影响
Pub Date : 2020-01-01 DOI: 10.26565/2075-5457-2020-34-2
Oxidative stress is considered to be the main mechanism of cadmium ions toxic effect on the cells and is caused by cadmium, as a non-transition metal, indirectly. Oxidative damage to cells due to the action of cadmium ions is tissue-specific and is associated with the antioxidant system inhibition, free heme accumulation and essential metals substitution in metalloproteins. Nitrogen monoxide (NO) exhibits high affinity for heme and proteins and peptides sulfhydryl groups, known to be the main molecular targets for cadmium ions. Taking all the above-mentioned into account, the aim of this work was to study the effect of NO radicals donors on the prooxidant-antioxidant state of mammalian tissues under oxidative stress caused by cadmium chloride administration in vivo. Male Wistar rats weighing 160–200 g were used in the study. CdCl2 was administered subcutaneously at a dose of 14 mg/kg body weight. The direct donor of the NO radical sodium nitroprusside (SNP, 1 mg/kg mass) and the substrate of the NO synthase reaction L-arginine (600 mg/kg mass) were administered intraperitoneally. In order to study the corrective action, donors of the NO radical were injected 0.5 h before the cadmium salt. The objects of investigation were blood plasma and liver, kidneys and spleen homogenates of rats. The cadmium chloride treatment caused a number of prooxidant-antioxidant balance disorders, most of which were revealed a day after injection. The accumulation of lipid peroxidation products was found in rat serum, liver, and spleen. The enhancement of prooxidant processes in these tissues may originate from cadmium ions and hemolysis products entry. In the antioxidant system, significant changes were observed under cadmium action only in the liver: an increase in the reduced glutathione content and SOD activity and a decrease in catalase activity. The precursor of nitric oxide L-arginine did not change the basal level of prooxidant-antioxidant parameters, and in most cases did not affect their dynamics in the organs studied after cadmium chloride administration. A direct NO donor, sodium nitroprusside, acted in liver and spleen mostly as a prooxidant. In liver, the injection of only nitroprusside, as well as the combined administration of SNP and CdCl2, led to free radical processes activation just in two hours. In spleen, the combined treatment by SNP and cadmium salt also caused an earlier development of oxidative stress, as witnessed by an increase in lipid hydroperoxides level and a decrease in reduced glutathione content. Therefore, the injection of a direct NO donor, sodium nitroprusside, and a substrate of NO synthase, L-arginine, in selected doses has insignificant corrective action on cadmium-induced oxidative stress in the liver, kidneys and spleen. However, in blood both donors of NO effectively prevented the accumulation of lipid peroxidation products under CdCl2 treatment; in addition, L-arginine significantly reduced the lactate dehydrogenase release,
氧化应激被认为是镉离子对细胞毒性作用的主要机制,是由镉作为非过渡金属间接引起的。镉离子对细胞的氧化损伤具有组织特异性,与抗氧化系统抑制、游离血红素积累和金属蛋白中必需金属的替代有关。一氧化氮(NO)对血红素、蛋白质和巯基具有很高的亲和力,而巯基是镉离子的主要分子靶点。综上所述,本研究旨在研究NO自由基供体对体内氯化镉氧化应激下哺乳动物组织促氧化-抗氧化状态的影响。实验选用体重160 ~ 200 g的雄性Wistar大鼠。CdCl2以14mg /kg体重皮下给药。NO自由基的直接供体硝普钠(SNP, 1 mg/kg质量)和NO合成酶反应底物l -精氨酸(600 mg/kg质量)腹腔注射。为了研究纠正作用,在镉盐注入前0.5 h注入NO自由基供体。研究对象为大鼠血浆及肝、肾、脾匀浆。氯化镉处理引起了一些促氧化-抗氧化平衡紊乱,大多数在注射后一天出现。在大鼠血清、肝脏和脾脏中发现脂质过氧化产物的积累。这些组织中促氧化过程的增强可能源于镉离子和溶血产物的进入。在抗氧化系统中,镉作用下仅在肝脏中观察到显著的变化:还原性谷胱甘肽含量和SOD活性增加,过氧化氢酶活性降低。一氧化氮前体l -精氨酸不改变促氧化-抗氧化参数的基础水平,在大多数情况下不影响其在氯化镉给药后的器官动力学。硝普钠是一氧化氮的直接供体,在肝脏和脾脏中主要起促氧化剂的作用。在肝脏中,仅注射硝普苷,以及SNP和CdCl2联合给药,仅在两小时内就导致自由基过程激活。在脾脏中,SNP和镉盐联合处理也导致了氧化应激的早期发展,表现为脂质氢过氧化物水平升高,还原性谷胱甘肽含量降低。因此,在一定剂量下注射NO直接供体硝普钠和NO合成酶底物l -精氨酸对镉诱导的肝、肾和脾氧化应激的纠正作用不显著。然而,在CdCl2治疗下,在血液中,一氧化氮供者有效地阻止了脂质过氧化产物的积累;此外,l -精氨酸显著减少乳酸脱氢酶的释放,这可能表明血细胞和血管对镉离子的损伤有保护作用。
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引用次数: 0
New records of tardigrade species of the Macrobiotus hufelandi group (Tardigrada: Eutardigrada: Macrobiotidae) from Ukraine 标题乌克兰hufelandi缓步动物群新记录种(缓步动物:准缓步动物:缓步动物科)
Pub Date : 2020-01-01 DOI: 10.26565/2075-5457-2020-34-10
Species of the Macrobiotus hufelandi group are among the most well known and commonly found tardigrades. They are reported in almost every study of Tardigrada diversity in terrestrial habitats. In spite of this, zoogeographical data on this group remain ambiguous and insufficient. Since most species of the M. hufelandi group have been described in the last three decades, many older records need to be confirmed or revised. In this study we aimed to investigate, which species may be present in Ukraine, since most Ukrainian records of this group are outdated. We studied samples of mosses and lichens collected from different regions of Ukraine (mainly in the East and South of the country) in 2007–2019. Tardigrades were extracted from samples and mounted on permanent slides in Faure's medium. After primary identification of genera and species groups we focused on 13 samples containing local populations of the M. hufelandi group. Details of animal and egg morphology were studied under high magnifications of phase contrast light microscopy. Species were identified based on morphology of the oral cavity armature, egg shell morphology, animal cuticle granulation, as well as some morphometric characters of claws and bucco-pharyngeal apparatus. This research revealed the presence of at least six different species in Ukraine: M. glebkai Biserov, 1990; M. hufelandi C.A.S. Schultze, 1834; M. macrocalix Bertolani & Rebecchi, 1993; M. polonicus Pilato, Kaczmarek, Michalczyk & Lisi, 2003; M. sottilei Pilato, Kiosya, Lisi & Sabella, 2012; M. vladimiri Bertolani, Biserov, Rebecchi & Cesari, 2011. Three of them: M. macrocalix, M. sottilei, and M. vladimiri are reported in this study from Ukraine for the first time. Adding three more species recorded in earlier studies: M. diversus Biserov, 1990; M. persimilis Binda & Pilato, 1972, and M. sapiens Binda & Pilato, 1984 – gives the total count of at least 9 species of the group in Ukraine. However, according to the recent studies on species complexes the real species diversity is very likely to be higher. Nevertheless, more sampling and obtaining molecular data from different Ukrainian populations of the M. hufelandi group from Ukraine are required.
hufelandi macrobious组的物种是最著名和最常见的缓步动物之一。在几乎所有关于陆地栖息地缓步动物多样性的研究中都有报道。尽管如此,关于这一群体的动物地理数据仍然不明确和不充分。由于M. hufelandi群的大多数物种都是在过去三十年中被描述的,因此许多较早的记录需要确认或修订。在这项研究中,我们的目的是调查哪些物种可能存在于乌克兰,因为乌克兰的大多数记录都是过时的。我们研究了2007-2019年在乌克兰不同地区(主要在该国东部和南部)收集的苔藓和地衣样本。从样品中提取缓步动物,并将其安装在福雷培养基中的永久载玻片上。在初步鉴定属群和种群后,我们将重点放在13个含有胡菲兰氏支原体种群的样本上。在高倍率的相衬光学显微镜下研究了动物和卵的形态细节。根据口腔电枢形态、蛋壳形态、动物角质层肉芽以及爪和颊咽器的一些形态特征进行了物种鉴定。这项研究揭示了乌克兰至少存在6种不同的物种:M. glebkai Biserov, 1990;M. hufelandi C.A.S. Schultze, 1834;M. macrocalx Bertolani & Rebecchi, 1993;M. polonicus Pilato, Kaczmarek, Michalczyk & Lisi, 2003;M. sottilei Pilato, Kiosya, Lisi & Sabella, 2012;M. vladimir Bertolani, Biserov, Rebecchi & Cesari, 2011。其中,M. macrocalix、M. sottilei和M. vladimiri为本文首次在乌克兰报道。增加早期研究记录的3个物种:M. diversus Biserov, 1990;M. persimilis Binda & Pilato, 1972年,以及M. sapiens Binda & Pilato, 1984年——给出了该类群在乌克兰至少9个物种的总数。然而,根据最近对物种复合体的研究,真实的物种多样性很可能更高。然而,需要从乌克兰的M. hufelandi群体的不同乌克兰人口中进行更多的采样和获得分子数据。
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引用次数: 0
The breeding distribution and numbers of the Jackdaw (Corvus monedula) in Kharkiv City, Ukraine 乌克兰哈尔科夫市寒鸦的繁殖分布和数量
Pub Date : 2020-01-01 DOI: 10.26565/2075-5457-2020-35-9
О. O. Brez, unova, O. Sinna
The counts of breeding Jackdaws were made in Kharkiv City, Ukraine in April – early June of 2016–2017. We estimated the breeding density among different habitat types. For such a purpose the city area was divided into 0.25 km² squares (n = 1354) and we estimated the numbers of pairs within the boundaries of 40 squares (QGIS program was used to choose randomly these squares). The surveys were made in April–May (19 squares) and early June (4 squares) in 2016 and April–May (10 squares) and early June (7 squares) in 2017. The surveys were made within main habitat types such as area of residential blocks of flats, residential area (1–2-storey buildings), industrial area, forest zone, park zone and undeveloped urban areas. The data on the area occupied by every major habitat type (in km²) were calculated by QGIS program. The 90 % of the territory of Kharkiv City was analysed, except water reservoirs and traffic way areas. The data of the surveys were extrapolated across areas of the same habitat types. Also we have searched for Jackdaw colonies within the boundaries of Kharkiv City to take these data into account. According to the collected data the Jackdaw prefers to nest in crevices of old 3–5-level buildings at built-up districts or in round cross-sections of posts at blocks-of-flats areas. Thus the mean density here was 22 pairs/km². The mean density of Jackdaw in industrial areas was 15 pairs/km², in undeveloped urban areas – 1.2 pairs/km², and in residential area (1–2-storey buildings) – 0.7 pairs/km². The total number of breeding Jackdaws in Kharkiv City was estimated at 2325 to 2630 pairs. The mean density of Jackdaws was 7.5 pairs/km². Taking into account the moderate decline in the population of the Jackdaws in some European countries (although in general the population trend remains stable within the continent), and the lack of the surveys of the breeding distribution and numbers of Jackdaws in large cities of the Eastern Ukraine, it’s reasonable to use our data as an optimal starting point for the monitoring of the regional population trend of the species. The data of 2016 year survey were used in European Breeding Bird Atlas 2 (EBBA2) programme (square 37UCR2).
2016-2017年4月至6月初,在乌克兰哈尔科夫市对繁殖的寒鸦进行了计数。估计了不同生境类型间的繁殖密度。为此,将城市区域划分为0.25 km²的正方形(n = 1354),我们估计了40个正方形边界内的配对数量(使用QGIS程序随机选择这些正方形)。调查时间为2016年4 - 5月(19个方格)和6月初(4个方格),2017年4 - 5月(10个方格)和6月初(7个方格)。调查范围包括主要生境类型,如住宅单位面积、住宅区(1 - 2层建筑)、工业区、林区、公园区和未开发市区。利用QGIS软件计算各主要生境类型所占面积(km²)。分析了哈尔科夫市90%的领土,除了水库和交通道路区域。调查的数据是在相同栖息地类型的地区外推的。此外,我们还在哈尔科夫市的边界内搜索了寒鸦的聚居地,以考虑这些数据。根据收集到的资料,寒鸦喜欢在建成区的3至5层旧楼宇的缝隙中筑巢,或在公寓楼的圆形横截面上筑巢。因此这里的平均密度是22对/km²。寒鸦在工业区的平均密度为15对/km²,在不发达的城市地区为1.2对/km²,在住宅区(1 - 2层建筑)为0.7对/km²。哈尔科夫市繁殖的寒鸦总数估计为2325至2630对。寒鸦的平均密度为7.5对/km²。考虑到一些欧洲国家的寒鸦种群数量适度下降(尽管总体上欧洲大陆内的种群趋势保持稳定),以及缺乏对乌克兰东部大城市的寒鸦繁殖分布和数量的调查,使用我们的数据作为监测该物种区域种群趋势的最佳起点是合理的。2016年调查数据用于欧洲种鸟图集2 (EBBA2)项目(平方37UCR2)。
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引用次数: 0
SOME FEATURES OF NEOPATRIMONIAL POLITICAL COMMUNICATIONS 新世袭政治传播的一些特征
Pub Date : 2020-01-01 DOI: 10.26565/2220-8089-2020-37-06
The article emphasizes the concept of neopatrimonial political regimes for specific features highlighting of political communications in third world countries since on the Afro-Asian material the traditional science-theoretical division into democratic, authoritarian and totalitarian regimes is not effective enough. Behind the formal signs of democratic regimes in many countries of the world, including in the countries of the former Soviet Union, lies the patrimonial logic of their functioning. The characteristic features of neopatrimonial regimes are distinguished: the “gap” between the center and the periphery, the construction of socio-political relations according to the patronage-client scheme, the dominant role of the state in the political system, “privatization” of state functions by representatives of the state-bureaucratic complex, turning them into a source of private income. It is noted that under this conditions in the political-communicative subsystem of the political system the specific model of power-oligarchic space is formed, within which presence of own media channels, the ability to control and censor information flows acquires on a special role. This contributes to the deployment of lobbying and corruption networks, which are becoming an integral mechanism for the functioning of neopatrimonial regimes. The problem of the peculiarity of the lexis in neopatrimonial communications is considered, namely, the widespread use of the so-called invective or obscene vocabulary. It is suggested that it is not caused by low educational or general cultural level of individual political actors, but by the tendency of cultivation the informal aspect of political communications inherent in neopatrimonial regimes. The question is posed that for further research on the specifics of neopatrimonial communications, one of the most promising approaches is the identification of the main models of neopatrimonial regimes and analysis of the features of their development.
由于在亚非材料上,传统的民主、专制和极权政体的科学理论划分已经不够有效,因此本文针对第三世界国家政治传播的具体特征,强调了新世袭政体的概念。在世界上许多国家,包括前苏联国家民主制度的正式迹象背后,存在着其运作的世袭逻辑。新世袭制政权的特征被区分开来:中心和边缘之间的“差距”,根据庇护-客户计划构建的社会-政治关系,国家在政治体系中的主导作用,国家-官僚复合体的代表对国家职能的“私有化”,将其变成私人收入的来源。需要指出的是,在这种条件下,在政治系统的政治沟通子系统中,形成了权力寡头空间的特定模式,在这种模式中,自己的媒体渠道的存在,控制和审查信息流的能力获得了特殊的作用。这有助于部署游说和腐败网络,这些网络正在成为新世袭政权运作的一个不可或缺的机制。本文讨论了新母系交际中词汇的特殊性问题,即所谓谩骂或淫秽词汇的广泛使用。这表明,这不是由于个别政治行为者的教育或一般文化水平低造成的,而是由于培养新世袭政权固有的政治沟通的非正式方面的趋势。提出的问题是,为了进一步研究新世系传播的具体情况,最有希望的方法之一是确定新世系制度的主要模式并分析其发展特点。
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引用次数: 0
The flora of the botanical preserve “Honcharivskyi” (Luhansk Region, Ukraine) “Honcharivskyi”植物保护区的植物区系(卢甘斯克地区,乌克兰)
Pub Date : 2020-01-01 DOI: 10.26565/2075-5457-2020-35-3
The current state of the flora of the botanical preserve “Honcharivskyi” (Svatove District, Luhansk Region) is analysed. The preserve includes three areas on the right-bank slopes of the Kobylka and the Krasna rivers with outcrops of chalk bedrocks. The study was conducted in summer and autumn 2019. Four expeditionary trips were organized to the areas near the villages of Oborotnivka and Nauholne. Floristic descriptions made by the line-transect method saved as research material. Identification of floristic composition was carried out by the plant species inventory along the transects. As a result, an annotated list of 85 species of vascular plants of 74 genera, 29 families, 3 classes and 2 divisions was compiled. In terms of systematic composition, the spectrum of main families is headed by Asteraceae (22 species, 25.9 % of the flora), Poaceae (9 species, 10.6 %), and Lamiaceae 7 (species, 8.2 %). These families include a significant number of ruderal, steppe and calcephilous species. Hemicryptophytes, represented by herbaceous plants of steppe meadows, prevail in the flora biomorphic structure (51 species, 60 %). Chamaephytes (in our case study the subshrubs, growing on steppe slopes, chalk and limestone outcrops, viz., Genista tinctoria L., Hyssopus cretaceus Dubjan., Teucrium polium L. etc.) are least presented (6 species, 7.1 %). A cenomorph analysis revealed predominance of the ruderal species (35; 41.2 %) due to grazing, trampling and economic activity in the study area. The flora ecological analysis was based on five main factors, which reflect climate and soil properties. In terms of soil acidity, the neutrophilic species formed a dominating group (53 species, 63 %), in terms of carbonate compounds – the acarbonatophilic (34 species, 40 %), in terms of soil salinity – the eutrophytic (36 species, 43 %), in terms of climate thermal regime – the sub-mesothermic (59 species, 70 %), and in terms of climate continentality – the hemicontinental ones (43 species, 52 %). Most recorded species prefer slightly acidic and/or neutral, rich in salts and carbonates, meadow like and common chernozems of steppe meadows and steppes. Twelve species of 11 genera and 7 families are of conservation concern. Of these, 10 species are listed in Red Data Book of Ukraine, five species are in the European Red List of Vascular Plants (Hyssopus cretaceus Dubjan., Artemisia hololeuca M. Bieb. ex Besser, Centaurea donetzica Klok., Cephalaria litvinivii Bobr., Hedysarum cretaceum Fisch.), and two species are in the Red List of plants of Lugansk Region (Thymus calcareus Klokov & Des.-Shost. and Potentilla erecta (L.) Raeusch.).
分析了卢甘斯克地区“Honcharivskyi”植物保护区(Svatove区)的植物区系现状。保护区包括科比尔卡河和克拉斯纳河右岸斜坡上的三个区域,露出白垩基岩。这项研究是在2019年夏季和秋季进行的。组织了四次远征旅行,前往奥博罗特尼夫卡村和瑙霍尔内村附近的地区。用样线法进行的植物区系描述保存为研究资料。植物区系组成通过样带植物物种清查进行鉴定。结果编制了维管植物2科3纲29科74属85种的带注释名录。在系统组成上,主要科以菊科(22种,占总区系的25.9%)、禾科(9种,10.6%)和Lamiaceae(7种,占总区系的8.2%)为主。这些科包括相当数量的原始、草原和钙质物种。以草原草甸草本植物为代表的半隐植物在植物区系生物形态结构中占优势(51种,占60%)。变色虫(在我们的案例中研究的是亚灌木,生长在草原斜坡上,白垩和石灰石露头,即,金尼斯塔·丁托利亚·L.,白垩纪·杜比扬。(Teucrium polium L.)最少(6种,7.1%)。形态分析显示,野生种占优势(35;41.2%),主要原因是放牧、践踏和经济活动。植物区系生态分析基于反映气候和土壤性质的5个主要因子。在土壤酸度方面,嗜中性物种占主导地位(53种,63%);在碳酸盐化合物方面,嗜碳物种占主导地位(34种,40%);在土壤盐度方面,富营养化物种占主导地位(36种,43%);在气候热状态方面,亚中热状态(59种,70%);在气候大陆性方面,半大陆性物种占主导地位(43种,52%)。大多数记录的物种喜欢微酸性和/或中性,富含盐和碳酸盐,草甸样和普通黑钙土的草原草甸和草原。有保护价值的有7科11属12种。其中10种被列入乌克兰红色数据手册,5种被列入欧洲维管植物红色名录(Hyssopus cretaceous Dubjan)。黄花蒿;前贝瑟,半人马座多涅茨卡Klok。;;;;;, Hedysarum白垩纪Fisch.), 2种被列入卢甘斯克地区植物红色名录(thyymus calcareus Klokov & Des.-Shost.)。和直立蕨(L.)Raeusch)。
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引用次数: 0
Effect of hemin and glutathione on some indicators of nitrogen and carbohydrate metabolism in rats 血红蛋白和谷胱甘肽对大鼠氮、碳水化合物代谢指标的影响
Pub Date : 2020-01-01 DOI: 10.26565/2075-5457-2020-34-3
The accumulation of heme in the organism under the influence of various hemolytic factors can cause the development of oxidative stress with the activation of free radical processes, oxidative damage to macromolecules and supramolecular complexes of cells and tissues. Under these conditions, the antioxidant defense system is activated in the organism, an important link of which is thiol compounds, particularly glutathione. Under such conditions, the processes of nitrogen and carbohydrate metabolism associated with the formation of adaptive reactions in response to stress have been investigated insufficiently. The aim of this work is to study some indicators of nitrogen and carbohydrate metabolism during the administration of hemin and the combined administration of hemin and glutathione to clarify the role of this antioxidant in the possible correction of metabolic processes. The subjects of the study were mature outbred albino male rats that received intraperitoneal injections of hemin (50 mg/kg) and glutathione (500 mg/kg) solutions, which was administered 0.5 hours before the introduction of hemin. The animals were tested 2 hours after hemin administration. The content of total and non-protein -SH groups, and the activity of gamma-glutamyltranspeptidase (GGT) in liver and kidney homogenates, glycogen content and tyrosinaminotransferase (TAT) activity in liver homogenate were studied. The content of reduced -SH groups can be an indicator of pro-antioxidant balance, GGT activity is one of the indicators of glutathione metabolism, and glycogen content and TAT activity in liver are hormone-sensitive indicators. The introduction of hemin caused a decrease in the content of total and non-protein -SH groups, glycogen content and an increase in TAT activity in liver, as well as an increase in the activity of GGT in this organ. Administration of glutathione to rats 30 minutes before the administration of hemin prevented shifts in these parameters in liver caused by the administration of hemin alone. In kidneys, an increase in the content of total -SH groups was found after the combined administration of glutathione and hemin compared with the effect of hemin alone. The results of this study may indicate a sensitivity of nitrogen and carbohydrate metabolism in rat organs to the effect of hemin and the corrective effect of glutathione under these conditions, probably mediated through an increase in the thiol component of the antioxidant defense system.
机体内血红素的积累在各种溶血因子的影响下,可引起氧化应激的发生,自由基过程被激活,对细胞和组织的大分子和超分子复合物造成氧化损伤。在这种情况下,机体的抗氧化防御系统被激活,其中一个重要环节是硫醇类化合物,尤其是谷胱甘肽。在这种条件下,氮和碳水化合物代谢过程与应激反应形成的适应性反应相关的研究还不够充分。本工作的目的是研究血红素和血红素与谷胱甘肽联合给药时氮和碳水化合物代谢的一些指标,以阐明这种抗氧化剂在可能的代谢过程纠正中的作用。研究对象为成熟的远交种白化雄性大鼠,在引入血红素前0.5小时,腹腔注射血红素(50 mg/kg)和谷胱甘肽(500 mg/kg)溶液。给药2小时后对动物进行测试。研究了肝脏和肾脏匀浆中总sh和非蛋白sh组含量、γ -谷氨酰转肽酶(GGT)活性、肝脏匀浆中糖原含量和酪氨酸氨基转移酶(TAT)活性。还原性sh基团的含量可作为促抗氧化平衡的指标,GGT活性可作为谷胱甘肽代谢的指标之一,肝糖原含量和TAT活性可作为激素敏感指标。血红素的引入导致肝脏中总sh和非蛋白sh含量降低,糖原含量增加,TAT活性增加,GGT活性增加。在给药前30分钟给大鼠注射谷胱甘肽,可以防止单独给药引起的肝脏这些参数的变化。在肾脏中,与单独给药相比,谷胱甘肽和血红素联合给药后发现总-SH组含量增加。本研究的结果可能表明,在这些条件下,大鼠器官中氮和碳水化合物代谢对血红素的作用和谷胱甘肽的纠正作用的敏感性,可能是通过抗氧化防御系统中硫醇成分的增加来介导的。
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引用次数: 0
Population frequency and risk factors for depression in Eastern Ukraine 乌克兰东部抑郁症的人口频率和危险因素
Pub Date : 2020-01-01 DOI: 10.26565/2075-5457-2020-35-7
The research is aimed to analysis of age-specific and gender-based risk factors for affective pathologies. Based on the population of the Kharkiv region, the population frequency of affective disorders was determined. It was calculated as probability for an individual to be affected throughout life and can be used for scientific purposes and genetic counseling. The age-specific cumulative frequencies were calculated, reflecting the risk for an individual to be affected in a specific period of life. They can be reference points in genealogical analysis. It has been shown that, despite a higher frequency in women, depressive disorders manifest earlier and tend to be more severe in men. That is, the female sex is a factor of increased risk, while affected men have a high background of genetic predisposition. To analyze risk factors, statistical material from specialized medical institutions of the Kharkiv region from 2010 to 2016 was used. The data were obtained from 1,199 patients who were hospitalized at the Institute of Neurology, Psychiatry and Narcology of the Academy of Medical Sciences of Ukraine, that is, they had extremely severe degrees of affective disorder and, probably, had a more significant genetic component in the structure of individual predisposition. The proportion of hospitalized women (74.9 %) was three times higher than the proportion of men (25.1 %) that significantly differs from the population sex ratio and indicates that the female sex is a factor of increased risk for affective pathology. The age of onset for affective disorders was lower in men than in women: bipolar disorder in males manifests 6 years earlier than in females, depressive episode – 2 years, recurrent depression – 5 years, chronic mood disorders – 4 years. On average, the difference between age of onset in women (46.6 years, 95% CI 45.7–47.5) and men (42.7 years, 95% CI 41.0–44.3) is 4 years. The maximum risk of affective disorder in women is between the age of 50 and 60 years, in men there are two peaks – at 20–30 and 45–60 years. The population frequency, that is an indicator of the risk for an affective disorder in the population of the Kharkiv region, is 0.21 %. The probability of affective disorder for men is 0.15 %, for women this indicator is 1.7 times higher – 0.26 %.
该研究旨在分析情感病理的年龄特异性和基于性别的风险因素。根据哈尔科夫地区的人口,确定了情感性障碍的人口频率。它被计算为个体在一生中受到影响的概率,可用于科学目的和遗传咨询。计算了特定年龄的累积频率,反映了个人在特定生命时期受到影响的风险。它们可以作为家谱分析的参考点。研究表明,尽管女性患抑郁症的频率更高,但男性的抑郁症表现得更早,而且往往更严重。也就是说,女性是风险增加的一个因素,而受影响的男性有很高的遗传易感性背景。采用哈尔科夫地区2010 - 2016年专业医疗机构的统计资料分析风险因素。这些数据是从乌克兰医学科学院神经病学、精神病学和麻醉学研究所住院的1 199名患者中获得的,也就是说,他们患有极其严重的情感障碍,并且可能在个人易感性结构中具有更重要的遗传成分。住院妇女的比例(74.9%)比男性的比例(25.1%)高出三倍,这与人口性别比例有显著差异,表明女性是情感病理风险增加的一个因素。男性情感障碍的发病年龄低于女性:男性双相情感障碍比女性早6年,抑郁发作2年,复发性抑郁症5年,慢性情绪障碍4年。平均而言,女性(46.6岁,95% CI 45.7-47.5)和男性(42.7岁,95% CI 41.0-44.3)的发病年龄相差4岁。女性情感障碍的最大风险出现在50至60岁之间,而男性则有两个高峰——20至30岁和45至60岁。人口频率是哈尔科夫地区人口中情感障碍风险的一个指标,为0.21%。男性患情感障碍的概率为0.15%,而女性的这一指标高出1.7倍,为0.26%。
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引用次数: 0
SIMULATION COMPONENT OF VIRTUAL MASS POLITICAL BEHAVIOUR IN RISK SOCIETY 风险社会中虚拟大众政治行为的模拟成分
Pub Date : 2020-01-01 DOI: 10.26565/2220-8089-2020-37-07
The simulation component of the manifestation of virtual political behaviour in risk society is considered. It was determined that using social networks, a new reality, which serves dubbing of political life of the modern individual. An important aspect of the study is to take into account the role of virtualization of modern political behavior in a society full of risks of both man-made and natural nature, information overload, substitution of facts and post-truth. Political behaviour simulation component digital origin plays an important role in shaping not only its real manifestation, but also in the structure of political consciousness that leaves a mark on the subsequent policy actions or activity of the modern individual. Particularly dangerous is the integration of this component with a risk society in which risks are increasingly gaining enormous proportions. After all, manipulative technologies, which are conveniently used by politicians or individual government officials, have a powerful psychological impact on the consciousness of the modern individual. It is not just voters, which is constantly flowing information pile of dirt during the electoral manipulation, which is essentially situational. Rather, we are talking about an ordinary individual who in the 21st century is so imbued with false information and its constant flow that it is really difficult for him to distinguish the truth. In the end - for the individual truth has ceased to be a value. It is proved that the virtualization of political behaviour contributes to its simulation, which consists in the imaginary active participation in the political life or society. A separate component of such behaviour is the tendency of man to irrationalism, low resistance to manipulative influences on the one hand, and the active use of social networks by government elites to form appropriate political thinking and political behavior on the other.
考虑了风险社会中虚拟政治行为表现的模拟成分。人们决定利用社会网络这一新的现实,为现代个人的政治生活提供配音服务。本研究的一个重要方面是考虑到现代政治行为虚拟化在一个充满人为和自然风险、信息超载、事实替代和后真相的社会中的作用。政治行为模拟组件数字起源不仅在塑造其真实表现方面发挥着重要作用,而且在政治意识结构中也发挥着重要作用,这种结构在现代个人随后的政策行动或活动中留下了印记。尤其危险的是将这一组成部分与风险日益增大的风险社会结合起来。毕竟,被政治家或个别政府官员方便使用的操纵技术,对现代个人的意识产生了强大的心理影响。不仅仅是选民,在选举操纵过程中,他们不断地流动着信息,堆积着污垢,这本质上是情境性的。相反,我们谈论的是一个普通人,他在21世纪充斥着虚假信息及其不断流动,以至于他很难区分真相。最终——因为个人的真理已不再是一种价值。事实证明,政治行为的虚拟化有助于其模拟,即假想的积极参与政治生活或社会。这种行为的一个单独组成部分是人的非理性主义倾向,一方面是对操纵影响的抵抗力较低,另一方面是政府精英积极利用社会网络形成适当的政治思想和政治行为。
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Visnik Kharkivs''kogo Natsional''nogo Universitetu Imeni VN Karazina Seriia Biologiia
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