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Yeast fungi of the genus Malassezia in dermatological diseases in animals 动物皮肤病中的马拉色菌属酵母菌
Pub Date : 2021-05-25 DOI: 10.33245/2310-4902-2021-165-1-50-57
A. Arkhypenko, V. Ushkalov
The relevance of a comprehensive study of yeast fungi of the genus Malassezia and the development of modern methods for the diagnosis of diseases caused by small domestic animals. Infectious diseases of small domestic animals caused by yeasts have become a high priority in recent years among the problems of veterinary mycology. However, issues of diagnosis, therapy and prevention of yeast infections in small pets remain poorly understood. Difficulties in diagnosing Malassezia infections lead to the fact that in most cases these diseases remain undiagnosed, sick animals do not receive adequate therapy. Therefore, there is a clear need to develop affordable and practical diagnostic algorithms. Depending on the severity and location of the lesion, systemic or local antifungal drugs are used in combination with symptomatic treatment: antihistamines, antipruritic drugs. If necessary, antibiotic therapy is used and in case of using systemic antifungal drugs, hepatoprotectors are prescribed. Malassezia-associated dermatitis in animals is usually a secondary problem due to an underlying skin disease, such as allergic dermatitis (including atopic canine dermatitis and flea dermatitis), recurrent bacterial pyoderma, and endocrine diseases (especially hypothyroidism). The diagnosis of Malassezia-associated dermatitis should be based on a combination of clinical manifestations and microbiological analysis. Treatment is usually selected depending on such factors as: form (localized or generalized), general state of health of the animal, the main primary disease. A review of literature sources on this issue. The characteristics of different species of the genus Malassezia, which are present on the skin of domestic animals and have an etiological significance in the occurrence of diseases of small domestic animals. The localization of yeast fungi on the body of sick animals is described. In Ukraine, these diseases have not been purposefully studied, and the awareness of veterinarians and humane physicians about them remains unsatisfactory.Key words: yeast, fungi of the genus Malassezia, dermatitis, otitis, infection.
马拉色菌属酵母真菌的综合研究与小型家畜引起的疾病的现代诊断方法的发展的相关性。近年来,由酵母引起的家畜传染病已成为兽医真菌学研究的热点问题之一。然而,诊断、治疗和预防小宠物酵母菌感染的问题仍然知之甚少。诊断马拉色菌感染的困难导致在大多数情况下,这些疾病仍未得到诊断,患病的动物没有得到适当的治疗。因此,显然需要开发负担得起且实用的诊断算法。根据病变的严重程度和位置,全身或局部抗真菌药物与对症治疗联合使用:抗组胺药、止痒药。如有必要,使用抗生素治疗,如果使用全身抗真菌药物,则开肝保护剂。动物马拉色菌相关性皮炎通常是由潜在皮肤病引起的继发问题,如过敏性皮炎(包括特应性犬皮炎和跳蚤皮炎)、复发性细菌性脓皮病和内分泌疾病(特别是甲状腺功能减退)。马拉色菌相关性皮炎的诊断应结合临床表现和微生物学分析。通常根据以下因素选择治疗方法:形式(局部或全身性)、动物的总体健康状况、主要原发疾病。对这一问题的文献来源进行综述。马拉色菌属不同种存在于家畜皮肤上的特征,对小家畜疾病的发生具有病原学意义。描述了病畜体内酵母菌的定位。在乌克兰,没有对这些疾病进行有目的的研究,兽医和人道医生对这些疾病的认识仍然不令人满意。关键词:酵母菌,马拉色菌属真菌,皮炎,中耳炎,感染
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引用次数: 0
Modern condition of the problem of Lime Burreliosis of animals (systematic review) 动物石灰性Burrelosis问题的现代状况(系统综述)
Pub Date : 2021-05-25 DOI: 10.33245/2310-4902-2021-165-1-64-78
O. Panteleenko, B. Yarchuk, T. Tsarenko
Lyme borreliosis (LB) is the most common tick-borne disease in the temperate climate of the Northern Hemisphere. LB is caused by spirochetes, which are grouped in the complex Borrelia burgdorferi sensu lato, vectors – Ixodidae mites, dozens of species of small mammals and birds actas reservoir hosts, and the role of reservoirs of domestic animals, such as dogs and cats, is not excluded. Although the ecology and epidemiology of the disease have been extensively studied in Europe and North America, there is considerable uncertainty regarding the study of Lyme borreliosis in veterinary medicine. Search, selection andanalysis of scientific data on the research topic were carried out according to acceptable rules for systematic reviews of the literature. The scientometric database Web of Science Core Collection, the database of scientific articles Pub Med and the database Scientific Periodicals of Ukraine were used. Seventy-four scientific articles were used for the article, which contained the necessary set of data and met the set goal. The article highlights the main issues of the etiology of the disease, which describes the characteristics of the pathogen, its properties, genotypic composition of Borrelia, which cause Lyme disease, the spread of pathogenic genotypes of Borrelia in Europe and Ukraine. The connection of Lyme borreliosis spread with ecological factors, climatic changes and anthropogenic impact on biocenoses and biotopes, the role of vectors and reservoir hosts in the spread of LB is described. Data on the prevalence and degree of infection of Ixodes mites with Borrelia, as well as the spread of LB among the population of Europe and Ukraine are presented. The seroprevalence of each of the animal species (dogs, cats, horses, ruminants), clinical manifestations of LB, if documented and indicators of LB prevalence among animals in Europe and Ukraine, as well as currently known treatments, prevention and diagnosis of LB animals are considered.Key words: Borrelia Burgdorferi Sensu Lato, Lyme Diseases Etiology, Ixodidae.
莱姆病是北半球温带气候中最常见的蜱传疾病。LB是由螺旋体引起的,这些螺旋体属于复杂的伯氏疏螺旋体、媒介——硬蜱科螨、数十种小型哺乳动物和鸟类——它们是宿主,而家畜(如狗和猫)宿主的作用也不排除。尽管欧洲和北美对莱姆病的生态学和流行病学进行了广泛研究,但兽医对莱姆虫病的研究仍存在相当大的不确定性。根据文献系统综述的可接受规则,对研究主题的科学数据进行了搜索、选择和分析。使用了科学计量数据库Web of Science Core Collection、Pub Med科学文章数据库和乌克兰科学期刊数据库。该文章使用了74篇科学文章,其中包含了必要的数据集,并达到了既定目标。文章重点介绍了该病病因的主要问题,描述了病原体的特征、特性、引起莱姆病的疏螺旋体的基因型组成、疏螺旋体致病基因型在欧洲和乌克兰的传播。描述了莱姆病传播与生态因素、气候变化和人为对生物群落和生境的影响、媒介和宿主在LB传播中的作用的联系。介绍了硬蜱螨感染疏螺旋体的流行率和程度,以及LB在欧洲和乌克兰人群中的传播数据。考虑了每种动物(狗、猫、马、反刍动物)的血清流行率、LB的临床表现(如果有记录的话)、欧洲和乌克兰动物中LB流行率的指标,以及目前已知的LB动物的治疗、预防和诊断。关键词:伯氏疏螺旋体,莱姆病病原学,硬蜱科。
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引用次数: 1
Analysis of changes in hematological indexes and essential trace elements metabolism under the using of vitamin-amino acid complex 维生素-氨基酸复合物对血液学指标及必需微量元素代谢的影响分析
Pub Date : 2021-05-25 DOI: 10.33245/2310-4902-2021-165-1-147-158
N. Vovkotrub, A. Melnyk, L. Bogatko, O. Piddubnyak, S. Sokolenko
It has been established that the level of blood parameters in the body of sheep is not always stable and often depends on the breed, sex, their physiological state, the level of productivity, feeding and housing conditions. Among the factors that determine the optimal sheep nutritional level, not the last place is occupied by their provision with a sufficient amount of essential amino acids, especially lysine, methionine, cystine, and vitamins, given their physiological importance for the animal body. The overwhelming amount of them is able to be synthesized in the sheep rumen with the help of microorganisms of the gastrointestinal tract, however, some of them are irreplaceable, that is, those whose balance must be replenished through feed rations. The article describes the use of the vitamin-amino acid complex "Аlphabet for Animals" in sheep, containing a complex of fat-and water-soluble vitamins, as well as amino acids, which contribute to the normalization of metabolism in the body of small ruminants, increase its resistance, have a positive effect on productivity, safety and reproductive animals functions. During the research, the effect of this feed additive on hemocytopoiesis and the exchange of essential micronutrients in ewes were studied. After a double use of the additive, an increase in the processes of erythrocytopoiesis was established, as evidenced by an increase in the number of blood erythrocytes by 21.2% and the hemoglobin content by 11.2%, stabilization of the size and degree of saturation of erythrocytes with hemoglobin, as evidenced by a decrease in MCH and MCV indices. There were no significant changes in the content of essential micronutrients in the blood serum of sheep under the influence of the "Alphabet for Animals", and a slight increase of zinc content in the blood was noted in sheep after the second injection of the drug. At the same time, in the blood of experimental animals group a significant increase the iron content on average up to 129.5±7.33 μg/100 ml (p<0.05) was established, and the number of animals with a low amount of blood iron was only 12.5 %. The number of leukocytes in the sheep blood of both groups was within the normal range and during the second and third blood tests did not change significantly.Key words: sheep, feeding, feed additives, amino acids, vitamins, hemocytopoiesis, essential micronutrients.
绵羊体内血液参数的水平并不总是稳定的,往往取决于品种、性别、生理状态、生产力水平、饲养和饲养条件。在决定绵羊最佳营养水平的因素中,提供足量的必需氨基酸,特别是赖氨酸、蛋氨酸、胱氨酸和维生素,并不是最后一个因素,因为它们对动物身体的生理重要性。其中绝大多数能在绵羊瘤胃中借助胃肠道微生物合成,但也有一些是不可替代的,即必须通过饲料定量补充其平衡。本文介绍了“动物用维生素-氨基酸复合物Аlphabet”在绵羊身上的应用,该复合物含有脂溶性和水溶性维生素,以及氨基酸,有助于小反刍动物体内代谢的正常化,增加其抵抗力,对动物的生产、安全和生殖功能有积极的影响。在试验中,研究了该饲料添加剂对母羊血细胞生成和必需微量元素交换的影响。双重使用后,红细胞生成过程增加,红细胞数量增加21.2%,血红蛋白含量增加11.2%,红细胞血红蛋白大小和饱和度稳定,MCH和MCV指数下降。在“动物字母表”的影响下,绵羊血清中必需微量营养素的含量没有明显变化,第二次注射药物后,绵羊血液中锌的含量略有增加。同时,实验动物组血液中铁含量显著升高,平均可达129.5±7.33 μg/100 ml (p<0.05),血铁含量低的动物仅占12.5%。两组绵羊血液中白细胞数量均在正常范围内,第二次和第三次血液检查无明显变化。关键词:羊,饲料,饲料添加剂,氨基酸,维生素,造血,必需微量元素
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引用次数: 0
Veterinary and sanitary characteristics of quail meat by feeding nanocrystalline cerium dioxide 用纳米二氧化铈饲养鹌鹑肉的兽医和卫生特性
Pub Date : 2021-05-25 DOI: 10.33245/2310-4902-2021-165-1-27-36
V. Zotsenko, V. Dzhmil, D. Ostrovskiy, A. Andriichuk, T. Melnyk
The unresolved problem of quail is feed stress and high sensitivity of poultry of this species to feed quality. To eliminate such consequences it is possible with the use of various biologically active feed additives. However, their introduction into the diet automatically raises questions about the quality and safety of the products for the consumer. The purpose of the research is to conduct a veterinary and sanitary assessment of the quality of quail meat for feeding NDC. The purpose of the research is to conduct a veterinary and sanitary assessment of the quality of quail meat for feeding NDC. The object of study were the quails of the Pharaoh breed, at the age of 24 were divided into two groups: experimental and control 30 heads each. The birds were kept in cages with free access to food and water. Quails of the experimental group were added to drinking water feed additive "Nanocerium" at a dose of 8.6 mg per liter of drinking water. Nanocerium feed additive is an aqueous dispersion of NDC with average nanoparticle sizes of 2–7 nm. Antemortem examination of quails of both groups revealed a satisfactory clinical condition of birds. The average weight of the gut carcass of quails of the experimental group was higher by 8.3% compared to the control. Examination of 20 carcasses of quails showed that they can be classified as first class. According to organoleptic parameters, quail meat is of good quality in veterinary and sanitary terms. Microbiological studies of quail meat indicate no effect of NDC in the applied doses on its bacterial contamination. Chemical parameters of meat (pH, aminoammonia nitrogen, volatile fatty acids during storage in the refrigerator) (5 days, t = 4–5 ° C) tended to increase and were within the normal range for fresh product. Tissue in quails of both groups was not detected. The biological value of quail meat in both groups was identical and no toxicity. Tasting evaluation of the broth and meat showed that NDC did not affect the studied taste. The obtained results show that the quails that received the feed additive "Nanocerium" with water according to the indicators of veterinary and sanitary examination are of good quality, which allows to use it in human food without restrictions.Key words: poultry, nanocerium, organoleptic evaluation, microbial contamination, biological value, tasting evaluation.
鹌鹑尚未解决的问题是饲料应激和对饲料质量的高度敏感。为了消除这种后果,可以使用各种生物活性饲料添加剂。然而,它们被引入到日常饮食中,自然而然地给消费者带来了有关产品质量和安全的问题。本研究的目的是对饲喂NDC的鹌鹑肉的质量进行兽医和卫生评估。本研究的目的是对饲喂NDC的鹌鹑肉的质量进行兽医和卫生评估。以24岁的法老品种鹌鹑为研究对象,分为实验组和对照组各30头。这些鸟被关在笼子里,可以自由获取食物和水。试验组鹌鹑以每升饮用水8.6 mg的剂量添加饮水饲料添加剂“纳米铈”。纳米铈饲料添加剂是NDC的水分散体,平均纳米颗粒尺寸为2 ~ 7 nm。两组鹌鹑的死前检查均显示鸟类临床状况满意。试验组鹌鹑内脏胴体平均重量较对照组提高8.3%。对20具鹌鹑尸体的检验表明,它们可以被归类为第一类。根据感官参数,鹌鹑肉具有良好的兽医和卫生品质。对鹌鹑肉的微生物学研究表明,应用剂量的NDC对其细菌污染没有影响。肉品的化学参数(pH、氨氮、挥发性脂肪酸)在冷藏5天(t = 4-5℃)有升高的趋势,在生鲜产品的正常范围内。在两组鹌鹑中均未检测到组织。两组鹌鹑肉的生物学价值完全相同,无毒性。肉汤和肉的品尝评价表明,NDC对所研究的味道没有影响。试验结果表明,经水处理的鹌鹑饲料添加剂“纳米铈”符合兽医和卫生检验指标,质量良好,可无限制地用于人类食品中。关键词:家禽,纳米铈,感官评价,微生物污染,生物学价值,味觉评价
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引用次数: 0
Spread and seasonal dynamics of dogs helminthiasis in BilaTserkva district BilaTserkva地区犬寄生虫病传播及季节动态
Pub Date : 2021-05-25 DOI: 10.33245/2310-4902-2021-165-1-119-128
I. Saichenko
Over the past decades, the number of dogs and cats has increased significantly on the territory of Ukraine. At each step, animals are in danger of non-contagious and contagious etiology. Parasitic diseases are almost half of the entire contagious pathologies of small animals. Number of invasive diseases depends on the following factors: season, age, sex, breeding features and also implementation and control of preventive measures against helminthiasis. Therefore, our research is to study thefeatures of distribution, seasonality, species composition and extensiveness of invasion of dog gelminthias on the territory of Bila Tserkva district, Kyiv region. The helminthocoproovoscopy research was carried out with a combined method standardized by G.O. Kotelnikov and V.M . Khrenov. The result of the study of faeces tests showed the problems of Bila Tserkva district with nematodoses and cestodoses of dogs. Regarding the species composition of the helminths we found, the helminths of the Nematoda class had a significant advantage: T. vulpis, T. canis, T. leonina, A. caninum, C. plica, U. stenocephala; among Cestoda class helminths, only D. caninum was diagnosed. Diagnosed pathogens of helminthiasis in dogs were mono- and myxinvasions. The research of seasonal dynamics of dog helminthosis revealed a high prevalence throughout the year. In summer, the helminthic prevalence of dogs reached the maximum index. In autumn, the intensity decrease. In winter, we received the minimum EI. In spring, the number of ill animals was increased. The species composition recorded by us in the spring was presented: T. vulpis and T. canis, both mono- and myxinvasion. In summer, T. vulpis, T. canis, C. plica, A. caninum, U. stenocephala. In winter, T. vulpis and T. canis dominate again. A feature of the autumn season for helminthiasis in dogs was the predominance of T. vulpis over other species of nematodes and cestodes in dogs. Regarding the age, sex and breed characteristics of the dog, the following results were obtained. The most comfortable were animals aged from birth to 7 years. Sex of animals (female, male), as shown by the results of our study, without affecting the infestation of helminthic infestations of dogs. As follows from the analysis of the results of helminthological studies, more susceptible are animals of the German Shepherd, Dachshund, Russian Spaniel breeds.Key words: epizootology, seasonality, dogs, helminthiasis, trichurosis, toxocariasis, intestinal strongylates, coproovoscopic studies, extensiveness of invasion.
在过去的几十年里,乌克兰领土上的狗和猫的数量显著增加。在每一步,动物都有非传染性和传染性的危险。寄生虫病几乎占小动物全部传染性疾病的一半。侵袭性疾病的数量取决于以下因素:季节、年龄、性别、繁殖特征以及对蠕虫病预防措施的实施和控制。因此,本研究旨在研究乌克兰基辅地区Bila Tserkva地区狗gelmintias的分布、季节性、物种组成和入侵范围等特征。采用G.O. Kotelnikov和v.m.标准化的联合方法进行蠕虫促卵泡镜研究。Khrenov。粪便试验研究的结果表明,比拉察尔克瓦地区存在狗的线虫和寄生虫中毒问题。从所发现的蠕虫种类组成来看,线虫纲的优势显著:vulpis、T. canis、T. leonina、a . caninum、C. plica、U. stenocephala;在足足纲蠕虫中,仅诊断出犬足纲蠕虫。犬寄生虫病的诊断病原体为单一和黏性侵袭。犬寄生虫病的季节动态研究表明,犬寄生虫病全年流行率较高。夏季犬类寄生虫患病率指数最高。在秋季,强度减弱。在冬季,我们收到的EI最低。到了春天,患病动物的数量增加了。我们在春季记录的物种组成为:单染型和混染型狐尾蛾和犬尾蛾。夏季主要有狐皮绦虫、犬皮绦虫、短背棘球绦虫、小头棘球绦虫。在冬季,狐尾绦虫和犬尾绦虫再次占主导地位。秋季犬寄生虫病的一个特点是狐尾绦虫在犬中比其他种类的线虫和绦虫占优势。关于犬的年龄、性别和品种特征,得到以下结果。最舒适的是出生到7岁的动物。动物的性别(雌性,雄性),如我们的研究结果所示,不影响蠕虫侵扰的狗。从蠕虫学研究结果的分析来看,德国牧羊犬、腊肠犬、俄罗斯猎犬品种的动物更容易受到感染。关键词:动物流行病,季节性,狗,寄生虫病,毛线虫病,弓形虫病,肠道强酰基酸盐,coprovoscopy研究,侵袭的广泛性
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引用次数: 0
Dynamics of biochemical bone and endothelial parameters during the replacement of bone defects in dogs with hydroxyapatite ceramic doped with silicon 掺硅羟基磷灰石陶瓷置换犬骨缺损过程中骨生化和内皮参数的动态变化
Pub Date : 2021-05-25 DOI: 10.33245/2310-4902-2021-165-1-191-200
M. Rublenko, V. Chemerovskіy, V. Vlasenko, N. Ulyanchich, P. Klimenko
Metal structures for osteosynthesis available in veterinary orthopedics are not able to compensate for the lost elements of bone tissue in complex splinter fractures. It is prompt the use of hydroxyappatite materials that replaced bone defects for maintenance of osteoconductive function, and ideally would combine osteointegration and osteoinductive properties. However, their influence on the biological processes of fracture consolidation which go through a number of successive stages and end with the formation of bone tissue in the fracture zone identical to the maternal, is insufficiently substantiated according to the criteria of the molecular biological phase of reparative osteogenesis. The aim of the study was to investigate the dynamics of biochemical osteotropic parameters and the level of NO using silicon-doped ceramics for fractures heeling in dogs. Materials and methods. The animals suffering of fractures that were admitted to the faculty clinic were divided into control (n=7) and experimental (n=7) groups. In both groups, extracortical osteosynthesis was performed with a support plate from an unalloyed titanium alloy. In the control group, bone defects were left to heal under spontaneous blood clot, and in the experimental group, they were replaced with ceramic based on hydroxyapatite with β-tricalciumphosphate doped with silicon (HA/β-TCP/l-Si–3).Blood samples were taken after the injury no later than the 48th day, and on the 3th, 12th, 21th, 42th and 60th days after osteosynthesis. To increase the objectivity of the biochemical analysis, we additionally formed a group of clinically healthy dogs that were admitted to the clinic for routine vaccination (n=10). It included the spectrophotometric determination of the content of NO, BALP, TRACP, Ca, P, Mg, total protein in blood serum, and fibrinogen in blood plasma. Research results. A clinical study showed that in the case of using HA/β-TCP/l-Si–3for splinter fractures, the stages of reparative osteogenesis are more optimized in time, and their consolidation occurs on average 19 days earlier than in the control group. The results of the biochemical study showed that when using HA/β-TCP/l-Si–3, it is accompanied by a peak NO value already on the third day, which is significantly higher than in the control group and indicates early angiogenesis in the research group. In terms of TRACP, the period of osteoresorption in the control group was permanent with little expressed peaks of activity. However, in the research group, the peak of TRACP activity is limited to 12 and 21 days, which is evidence of an optimized inflammatoryresorptive phase. In parallel with this, the activity of BALP increases, which indicates the consistency of the stages of reparative osteogenesis and provides an optimized and accelerated consolidation of fractures in the research group. Conclusion. The dynamics of NO, BALP and TRACP pathochemically substantiates the optimized reparative osteogenesis when using HA/β-TCP/l
兽医骨科中可用的骨合成金属结构不能补偿复杂碎片骨折中丢失的骨组织元素。羟基磷灰石材料可替代骨缺损维持骨传导功能,理想情况下可结合骨整合和骨诱导特性。然而,根据修复性成骨的分子生物学阶段的标准,它们对骨折巩固的生物学过程的影响还没有得到充分证实。骨折巩固经历了许多连续的阶段,最终以与母体相同的骨折区形成骨组织结束。本研究旨在探讨掺硅陶瓷对犬骨折愈合的生化致骨参数和一氧化氮水平的影响。材料和方法。将入院的骨折动物分为对照组(n=7)和实验组(n=7)。两组均采用非合金钛合金支撑板进行牙槽外植骨。在对照组中,骨缺损在自发血凝块下自行愈合,在实验组中,用掺杂硅的β-三磷酸钙羟基磷灰石陶瓷(HA/β-TCP/ l-Si-3)代替骨缺损。伤后不迟于第48天及骨固定后第3天、第12天、第21天、第42天和第60天采血。为了增加生化分析的客观性,我们另外组成了一组临床健康的狗,这些狗被送到诊所进行常规疫苗接种(n=10)。分光光度法测定血清NO、BALP、TRACP、Ca、P、Mg、总蛋白和血浆纤维蛋白原含量。研究的结果。临床研究表明,采用HA/β-TCP/l- si - 3治疗碎片性骨折时,修复性成骨的分期更及时,其愈合时间比对照组平均提前19天。生化实验结果显示,在使用HA/β-TCP/ l-Si-3时,在第3天已出现NO峰值,明显高于对照组,提示研究组血管生成较早。在TRACP方面,对照组的骨吸收时间是永久性的,几乎没有活性表达峰。然而,在研究组中,TRACP活性的峰值被限制在12天和21天,这是炎症吸收期优化的证据。与此同时,BALP的活性增加,这表明修复性成骨阶段的一致性,并在研究组中提供了优化和加速的骨折巩固。结论。HA/β-TCP/ l-Si-3用于管状骨碎裂骨折的骨缺损置换时,NO、BALP和TRACP的动态病理证实了其最佳的修复性成骨效果。关键词:骨标志物,骨碱性磷酸酶同工酶,NO,抗酒石酸酸性磷酸酶,纤维蛋白原,钙,磷。
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引用次数: 1
Influence of the prebiotic Bio-active on natural resistance, safety and productivity of young pigs 益生元生物活性对仔猪自然抗性、安全性和生产能力的影响
Pub Date : 2021-05-25 DOI: 10.33245/2310-4902-2021-165-1-37-49
V. Lyasota, V. Malyna, L. Bondarenko, V. Bolokhovska, Y. Balatsky, N. Bukalova, N. Bogatko, O. Khitska, S. Tkachuk
The primary task of the livestock industry is to provide the population with high-quality food products, and the industry with raw materials. For this, it is necessary to ensure a high level of productivity and safety of young pigs. Studies carried out in recent years indicate an increase in the incidence of gastrointestinal diseases among young farm animals, leading to a decrease in the immunobiological reactivity of the piglets' organism and significant damage to farms. When performing the research, we used zootechnical, zoohygienic, biochemical, immunological and variational-statistical research methods. Research work was carried out during 2010-2018 at the Department of Animal Hygiene and Fundamentals of Veterinary Medicine of the Bila Tserkva National Agrarian University. Scientific and economic experiments and production tests were carried out in the farms of Progress LLC (Uzin, Bila Tserkva district) and Denisenko LLC (Skvirsky district, Kyiv region). Experimental studies were carried out in the Problem Laboratory of Immunology, Department of Animal Hygiene and Fundamentals of Veterinary Medicine, interfaculty laboratory of biochemical and histochemical research methods. For the first time, the prebiotic Bio-active was used as a feed additive in the diet of young pigs on growing at industrial keeping, its positive effect on the safety, productivity, physiological and immunological state of their body was proved. For the first time, the optimal dose of the prebiotic Bio-active for growing pigs was established and a method of its use was developed. The optimal dose of the prebiotic Bio-active for growing young pigs is 5 g (5x107 CFU) per 10 kg of body weight when fed with compound feed once a day for 30 days, which contributes to an increase in the average daily weight gain of piglets by 17.2 % (P <0.01) and 16.6 % (P <0.05) on the 30th and 60th days of the study, respectively. Feeding pigs with Bio-active promotes an increase in the content of total blood serum protein by 7.2 % (P <0.05), albumin – by 5.2 %, γ-globulins – by 6.3 %, an increase in AST activity – by 23, 1 % (P <0.05) and ALT – by 22.4 % (P <0.01). In the peripheral blood of rearing pigs under the influence of the prebiotic Bio-active, the proliferation, differentiation and specialization of immunocompetent cells increase: an increase in the total number of T-lymphocytes by 5.43 % (P <0.05), B-lymphocytes by 2.85 %, a decrease in the content of 0-lymphocytes – by 8.29 % (P <0.05). The number of medium-avid T-lymphocytes also increases – by 9.19 % (P <0.05) and B-lymphocytes – by 10.28 % (P <0.05) due to a decrease in the level of low-avid immunocompetent cells. The use of the prebiotic Bio-active in an optimal dose promotes the activation of metabolic processes, antigen-nonspecific immunity and an increase in the body weight of pigs.Key words: industrial pig breeding, rearing young pigs, housing conditions, gastrointestinal canal, prebiotic, immunobiological reactivity, metab
畜牧业的首要任务是为人口提供高质量的食品,并为该行业提供原材料。为此,有必要确保幼猪的高水平生产力和安全性。近年来进行的研究表明,幼年农场动物胃肠道疾病的发病率增加,导致仔猪机体的免疫生物反应性降低,并对农场造成重大损害。在进行研究时,我们使用了动物技术、动物卫生、生物化学、免疫学和变分统计研究方法。2010-2018年期间,比拉·泽尔科娃国立农业大学动物卫生和兽医基础系开展了研究工作。在Progress LLC(Bila Tserkva区Uzin)和Denienko LLC(基辅地区Skvirsky区)的农场进行了科学和经济实验以及生产测试。实验研究在动物卫生与兽医基础系免疫学问题实验室、生物化学和组织化学研究方法学科间实验室进行。首次将益生元生物活性物质作为饲料添加剂应用于工业饲养仔猪的日粮中,证实了其对仔猪的安全性、生产性、生理免疫状态等方面的积极作用。首次确定了用于生长猪的益生元生物活性剂的最佳剂量,并开发了其使用方法。当每天用复合饲料喂养30天时,生长猪的益生元生物活性剂的最佳剂量为每10公斤体重5克(5x107 CFU),这有助于在研究的第30天和第60天仔猪的平均日增重分别增加17.2%(P<0.01)和16.6%(P<0.05)。用生物活性物质喂猪可使血清总蛋白含量增加7.2%(P<0.05),白蛋白增加5.2%,γ-球蛋白增加6.3%,AST活性增加23.1%(P<0.01),ALT增加22.4%(P<0.01,免疫活性细胞的分化和特化增加:T淋巴细胞总数增加5.43%(P<0.05)、B淋巴细胞总数增加2.85%、T淋巴细胞总数减少5.43%(P<0.01),由于低狂热免疫活性细胞水平的降低,中等狂热T淋巴细胞的数量也增加了9.19%(P<0.05),B淋巴细胞的数量增加了10.28%(P<0.05)。以最佳剂量使用益生元生物活性物质可促进代谢过程的激活、抗原非特异性免疫和猪体重的增加。关键词:工业养猪,育幼,饲养条件,胃肠道,益生元,免疫生物反应性,代谢,安全。
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引用次数: 0
Morphological and biochemical parameters of the blood of broiler chickens with ornithobacteriosis, and for the use of Poledoxin and Tilmox 25% 对禽杆菌病肉鸡的血液形态学和生化指标进行了测定,并对25%多氧多辛和Tilmox进行了测定
Pub Date : 2021-05-25 DOI: 10.33245/2310-4902-2021-165-1-165-178
A. Tyshkivska, V. Dukhnitsky, M. Tyshkivsky
The article presents the results of studies of morphological and biochemical parameters of blood of broiler chickens KOBB-500, patients with ornithobacteriosis, who were not treated, and sick birds, for the treatment of which were used drugs "Poledoxin" (active substance - doxycycline hyclate) and "Tilmox 25%" (active substance - tilmicosin phosphate). Studies have shown that ornithobacteriosis in broiler chickens is characterized by erythrocytopenia, hypohemoglobinemia and decreased hematocrit. In the birds of the experimental groups, which in accordance with the treatment regimen used poledoxin and tilmox 25% erythrocyte count, hemoglobin content in the blood and hematocrit at 216 h of the experiment were higher than in the control: erythrocyte count - 19 and 36%, respectively; hemoglobin content - by 17% in poultry of both experimental groups; hematocrit - by 19 and 23%, respectively (p≤0.05). In the serum proteins of patients with ornithobacteriosis of broiler chickens of the control group was found dysproteinemia, in which at 216 h of the experiment there was a decrease in total protein and albumin by 12 and 34%, respectively. At the same time there was an increase in the content of globulins by 32%. The content of total protein and albumin in the serum of poultry 2 and 3 experimental groups increased and by 216 h exceeded the control index by 24 and 33% (p≤0.05), respectively, albumin - by 56% in poultry of both experimental groups. The content of globulins in the serum of poultry of the experimental groups for the period from 24 to 216 h of the experiment was in the range of 10.72 - 11.98 g / l, while in the control - 11.32 - 15.21 g / l. In the serum of broiler chickens of the control group for the period from 24 to 216 h increased the activity of alanine aminotransferase by 14%, while in the serum of poultry of experimental groups treated with poledoxin and tilmox 25% its activity decreased by 96, 120 and 216 h was probably less than in the control. Hypocalcemia was observed in the control group of broiler chickens ornithobacteriosis, whereas in the serum of poultry 2 and 3 experimental groups treated, the total calcium content in the period from 24 to 216 h was probably higher than in the control. Hypophosphatemia was observed in the control and 2 experimental birds (poledoxine), and the inorganic phosphorus content in their serum was 216 h lower than in the experimental group 3 birds (tilmox 25% was used) by 43 and 23%, respectively.Key words: doxycycline, tilmicosin, respiratory diseases of poultry, O. Rhinotracheale, antibiotics, bioavailability.
本文介绍了对KOBB-500肉鸡、未经治疗的鸟类细胞菌病患者和使用药物“多西环素”和“Tilmox25%”治疗的病禽的血液形态和生化参数的研究结果。研究表明,肉鸡禽细菌素病的特点是红细胞减少、低血红蛋白血症和红细胞压积降低。在按照治疗方案使用波多辛和替莫的实验组鸟类中,25%的红细胞计数、216小时血液中血红蛋白含量和红细胞压积均高于对照组:红细胞计数分别为-19%和36%;血红蛋白含量——在两个实验组的家禽中都增加了17%;红细胞压积-分别降低19%和23%(p≤0.05)。对照组肉鸡的鸟类细胞菌病患者血清蛋白质中发现异常蛋白血症,其中在实验的216h,总蛋白质和白蛋白分别降低了12%和34%。同时球蛋白的含量增加了32%。第2和第3个实验组的家禽血清中总蛋白和白蛋白含量在216小时时分别比对照指数增加了24%和33%(p≤0.05),白蛋白含量在两个实验组中都增加了56%。试验组肉鸡血清中球蛋白含量在试验的24~216小时内分别为10.72~11.98克/升和11.32~15.21克/升,而在用波多辛和替莫司25%处理的实验组家禽血清中,其活性在96h、120h和216h时的下降可能低于对照组。肉鸡鸟粪菌病对照组观察到低钙血症,而在处理的家禽2和3个实验组的血清中,24至216小时的总钙含量可能高于对照组。在对照组和2只实验鸟(波多辛)中观察到低磷酸盐血症,其血清中无机磷含量在216小时内分别比实验组3只(使用25%的替莫)低43%和23%。关键词:多西环素,替米考星,家禽呼吸道疾病,鼻杆菌,抗生素,生物利用度。
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引用次数: 0
Coagulase-positive staphylococci in dogs and their antimicrobial resistance (systematic review) 犬中凝固酶阳性葡萄球菌及其耐药性研究(系统综述)
Pub Date : 2021-05-25 DOI: 10.33245/2310-4902-2021-165-1-104-118
M. Shevchenko, M. Savcheniuk, B. Yarchuk, N. Sakhniuk, T. Tsarenko
Staphylococcus spp. a common genus of bacteria that colonizes the body of dogs. Some families of this genus are pathogenic and are involved in the development of diseases of various systems and organs, especially the skin and outer ear. One of the main signs of the most pathogenic families of this genus is the secretion of the enzyme coagulase. They are grouped into the group of coagulase-positive Staphylococcus (CoPS). S. pseudintermedius most often is isolated from a dog, about 10-60% isolates. S. aureus is identified less frequently, in 4-15% of cases, but it is associated with people who are its natural carriers. S. schleiferi subsp. coagulans is another family that can be identified in the pathological material obtained from dogs. However, coagulase is one of much different pathogenicity factors in this family. Also, microorganisms of this family can exist both in the form of a planktonic culture and in the structure of a biofilm, on which the effect of antibacterial agents is weakened. Some strain of CoPS carry genes of resistance to various antibacterial agents and may occupy a dominant position in the pathogenic process. Such strains can colonize the environment in places where animals congregate, which leads, for example, to the occurrence of clinical infections. Also resistant are strains of this family that can colonize other animals or humans. Owners and specialists working with animals are constantly at risk. To date, the issue of the emergence of resistance to beta-lactam antibiotics (methicelin-resistant strains) and the acquisition of resistance to several groups of antibacterial agents is acute. In the countries of the European Union, a fairly large number of studies are conducted aimed at studying the spread of Staphylococcus spp. analysis of virulence and pathogenicity factors, as well as mechanisms of acquiring antibacterial resistance. Programs to control the prevalence of resistant strains are also being developed there. Different authors show resistance to a large number of antibacterial agents, the profile of antibacterial resistance may differ both within different groups and between different drugs of the same pharmaceutical group. In Ukraine, there are no systematic studies of the spread of resistant strains of microorganisms among small animals.Key words: Staphylococcus spp., S. pseudintermedius, S. aureus, S. schleiferi subsp. Coagulans, CoPS, MRSP, MRSA, antibacterial resistance, dogs.
葡萄球菌属:一种常见于狗体内的细菌属。该属的一些科具有致病性,并与各种系统和器官的疾病发展有关,特别是皮肤和外耳。这个属中最具致病性的科的主要标志之一是酶凝固酶的分泌。它们被归为凝固酶阳性葡萄球菌(cop)组。假中间链球菌最常从狗身上分离出来,约有10-60%的分离株。金黄色葡萄球菌的发现频率较低,在4-15%的病例中,但它与作为其天然携带者的人有关。施莱氏梭菌亚种凝血菌是另一个可以在狗的病理材料中发现的家族。然而,凝血酶是该家族许多不同的致病因子之一。此外,这个家族的微生物既可以以浮游培养的形式存在,也可以在生物膜的结构中存在,在生物膜的结构中,抗菌剂的作用减弱了。某些菌株携带对多种抗菌药的耐药基因,可能在致病性过程中占据主导地位。这些菌株可以在动物聚集的地方定居,例如导致临床感染的发生。这个家族的菌株也具有抗药性,它们可以在其他动物或人类身上定居。与动物打交道的主人和专家总是处于危险之中。迄今为止,对-内酰胺类抗生素(甲氧西林耐药菌株)产生耐药性和对几组抗菌剂产生耐药性的问题非常严重。在欧盟国家,进行了相当多的研究,旨在研究葡萄球菌的传播,分析其毒力和致病性因素,以及获得耐药性的机制。控制耐药菌株流行的规划也正在那里制定。不同的作者对大量的抗菌药物表现出耐药性,在不同的组内和同一药物组的不同药物之间,抗菌药物的耐药情况可能不同。在乌克兰,没有对小动物中耐药微生物菌株传播的系统研究。关键词:葡萄球菌,假中间葡萄球菌,金黄色葡萄球菌,施莱氏葡萄球菌凝血剂,cop, MRSP, MRSA,抗菌素耐药性,狗。
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引用次数: 1
An overview of seizures and epilepsy in rabbits: etiological differences and clinical management 概述癫痫发作和癫痫在兔子:病因差异和临床管理
Pub Date : 2021-05-25 DOI: 10.33245/2310-4902-2021-165-1-159-164
E. Gülersoy, S. İyigün, B. Erol
World Health Organization data suggest that neurological disorders are an important and growing cause of morbidity. One of the most common neurological disorder affecting people is epilepsy. Many companion animal neurological diseases share epidemiologic, pathophysiologic and clinical features with their human counterparts. In companion animals, affected species are mostly dogs, cats and rabbits. Seizure is defined as the clinical manifestation of abnormal electrical activity in the brain. Epilepsy is a brain disease characterized by the psychological, cognitive, social and environmental consequences of seizures. The epileptic seizures are recurrent events characterized by behavioral alterations that reflect the underlying neural mechanisms of the disease. In most cases, the disease can be diagnosed by anamnesis or observing the seizure. There are many reviews and researches about epilepsy and epileptic seizures in companion animals such as dogs and cats but not in rabbits. There are several causes of epilepsy in rabbits including viral, bacterial, parasitic, metabolic, respiratory, cardiovascular, nutritional, toxic, traumatic, enviromental and non-epileptic causes. Rabbits can be considered suitable for seizure and epilepsy investigations due to their recurrent seizures with low risk of death. As mentioned, there are several causes of epilepsy in rabbits but still to elucidate the exact mechanism of epilepsy and epileptic seizures in rabbits more studies need to be carried out. Despite the advances in the disease management, epilepsy is still an important cause of disability and mortality in both humans and companion animals. As tonic-clonic seizures with brainstem origin mostly affect children, epileptic seizures in rabbits may be a good model for further studies.Key words: Brain disease, epilepsy, neural disturbances, behavioral alterations, rabbit, seizure.
世界卫生组织的数据表明,神经系统疾病是一个重要且日益增长的发病原因。影响人类的最常见的神经系统疾病之一是癫痫。许多伴侣动物神经系统疾病与人类具有相同的流行病学、病理生理和临床特征。在伴侣动物中,受影响的物种主要是狗、猫和兔子。癫痫被定义为脑电活动异常的临床表现。癫痫是一种脑部疾病,其特点是癫痫发作的心理、认知、社会和环境后果。癫痫发作是反复发作的事件,其特征是行为改变,反映了该疾病的潜在神经机制。在大多数情况下,这种疾病可以通过记忆或观察癫痫发作来诊断。关于狗、猫等伴侣动物的癫痫和癫痫发作的综述和研究较多,但对家兔的研究较少。兔子的癫痫有几种原因,包括病毒、细菌、寄生虫、代谢、呼吸、心血管、营养、毒性、创伤、环境和非癫痫原因。家兔可被认为适合癫痫发作和癫痫调查,因为它们的反复发作和低死亡风险。如前所述,家兔癫痫的病因有多种,但为了阐明家兔癫痫和癫痫发作的确切机制,还需要进行更多的研究。尽管在疾病管理方面取得了进展,但癫痫仍然是人类和伴侣动物致残和死亡的重要原因。由于脑干源性强直阵挛性发作多发生在儿童身上,因此家兔癫痫发作可能是进一步研究的一个很好的模型。关键词:脑部疾病,癫痫,神经障碍,行为改变,兔子,癫痫发作
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Naukovii visnik veterinarnoyi meditsini
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