Pub Date : 2018-12-24DOI: 10.33245/2310-4902-2018-144-2-13-19
M. L. Radsikhovskii, L. Goralskii, B. Borissevich, O. Dyshkant
In this article to be spoken about indicators of action of coronaviridae on an organism of animals – qualitative and quan-titative characteristics of blood. These indicators change at many patological reactions and participate in ensuring nonspe-cific and specific resistance of an organism. With the help of the analysis of integral leukocyte indices based on the formula of blood reflecting the state of neurohumoral homeostasis and immunological reactivity of the organism, the violations were first discovered and established humoral and cellular links of the immune system, micro- and macrophage system, reduction of nonspecific protection of the organism and revealed connections between integral hematological parameters and immunity indexes in dogs with experimental and natural infection with coronavirus. In animals in the natural course of coronavirus enteritis there were changes in the indexes of intoxication: a significant increase in the leukocyte index of intoxication, the indicator of reactivity of the organism, the index of leukocyte shift of blood and the index of intoxication, and significant decrease in the nuclear index, the general index and the leukocyte index in indices of nonspecific reactivity, a significant increase in the ratio of neutrophils and lymphocytes, the ratio of neutrophils and monocytes, the ratio of eosinophils to leukocytes and the ratio of segmental neutrophils and bands neutrophils, a significant decrease in the ratio of lymphocytes and monocytes, the index of ratio of lymphocytes and eosinophils, the index of Garkavy, index allergy and immunoreactivity index in indexes of activity of inflammation, a significant decrease of the ratio of leukocytes and ЕSR, the lymphocytic granulocytic index and the ratio of lymphocytes and monocytes to the ESR. Experimental infection revealed changes in the indexes of intoxication: a significant increase in the leukocyte index of intoxication, the nuclear index and the index of blood leukocyte shift, a significant decrease in the indicator of reactivity of the organism, the general index, the leukocyte index and the indicator of intoxication in the indices of nonspecific reactivity, a significant increase in the ratio of neutrophils and lymphocytes to the index ratio of eosinophils to leukocytes, a significant decrease in the ratio of lymphocytes and monocytes, the index of ratio of lymphocytes and eosinophils, the Garkavy index, the ratio of segmental neutrophils and bands neutrophils and the index of immunoreactivity in the activity indices, a significant decrease in the ratio of leukocytes and ESR and lymphocytic granulocytic index. Integral hematological parameters of peripheral blood have been proved to increase the informative value of the general analysis of blood in dogs with infectious diseases and allow to determine not only the degree of reactivity of the organism, but also to assess the level of endogenous intoxication. Key words: coronavirus enteritis, natural infe
{"title":"Integral indexes of intoxication in caninae coronaviridae enteritis","authors":"M. L. Radsikhovskii, L. Goralskii, B. Borissevich, O. Dyshkant","doi":"10.33245/2310-4902-2018-144-2-13-19","DOIUrl":"https://doi.org/10.33245/2310-4902-2018-144-2-13-19","url":null,"abstract":"In this article to be spoken about indicators of action of coronaviridae on an organism of animals – qualitative and quan-titative characteristics of blood. These indicators change at many patological reactions and participate in ensuring nonspe-cific and specific resistance of an organism.\u0000\u0000With the help of the analysis of integral leukocyte indices based on the formula of blood reflecting the state of neurohumoral homeostasis and immunological reactivity of the organism, the violations were first discovered and established humoral and cellular links of the immune system, micro- and macrophage system, reduction of nonspecific protection of the organism and revealed connections between integral hematological parameters and immunity indexes in dogs with experimental and natural infection with coronavirus.\u0000\u0000In animals in the natural course of coronavirus enteritis there were changes in the indexes of intoxication: a significant increase in the leukocyte index of intoxication, the indicator of reactivity of the organism, the index of leukocyte shift of blood and the index of intoxication, and significant decrease in the nuclear index, the general index and the leukocyte index in indices of nonspecific reactivity, a significant increase in the ratio of neutrophils and lymphocytes, the ratio of neutrophils and monocytes, the ratio of eosinophils to leukocytes and the ratio of segmental neutrophils and bands neutrophils, a significant decrease in the ratio of lymphocytes and monocytes, the index of ratio of lymphocytes and eosinophils, the index of Garkavy, index allergy and immunoreactivity index in indexes of activity of inflammation, a significant decrease of the ratio of leukocytes and ЕSR, the lymphocytic granulocytic index and the ratio of lymphocytes and monocytes to the ESR.\u0000\u0000Experimental infection revealed changes in the indexes of intoxication: a significant increase in the leukocyte index of intoxication, the nuclear index and the index of blood leukocyte shift, a significant decrease in the indicator of reactivity of the organism, the general index, the leukocyte index and the indicator of intoxication in the indices of nonspecific reactivity, a significant increase in the ratio of neutrophils and lymphocytes to the index ratio of eosinophils to leukocytes, a significant decrease in the ratio of lymphocytes and monocytes, the index of ratio of lymphocytes and eosinophils, the Garkavy index, the ratio of segmental neutrophils and bands neutrophils and the index of immunoreactivity in the activity indices, a significant decrease in the ratio of leukocytes and ESR and lymphocytic granulocytic index.\u0000\u0000Integral hematological parameters of peripheral blood have been proved to increase the informative value of the general analysis of blood in dogs with infectious diseases and allow to determine not only the degree of reactivity of the organism, but also to assess the level of endogenous intoxication.\u0000\u0000Key words: coronavirus enteritis, natural infe","PeriodicalId":34230,"journal":{"name":"Naukovii visnik veterinarnoyi meditsini","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2018-12-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47697915","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2018-12-24DOI: 10.33245/2310-4902-2018-144-2-20-27
S. Babyuk, O. Piskùn, V. Ukhovskyi, A. Piskun, L. Korniienko, T. Tsarenko
Leptospirosis – common to humans and animals is a dangerous infectious disease that is caused by microbes - leptospires. The disease is accompanied by fever, kidney damage, liver, cardiovascular and nervous system. Leptospirosis in dogs is considered one of the most common diseases. Particularly difficult is the breed with a faulty type of body structure, such as: Neapolitan Mastino, Bulmastiff, English Bulldog, French Bulldog, Boxer, Bologna, Bloodhound, Basset Hound. The disease is most often diagnosed in dogs of hunting breeds, as a result of frequent contact with standing water, as well as in courtyard and stray dogs. Young animals and puppies get sick more often, as they do not have a stable immunity, the hemorrhagic form is more often diagnosed in older dogs. The subject was to study the distribution of leptospirosis among dogs, to establish the seropositivity level and to determine the seroprevalence of the most common of Leptospira serotypes that circulate among this species of animals. For research, an extensive diagnostic series of L. interrogans which includes 20 serovars, and blood serum from dogs that were selected in veterinary clinics in the city of Kyiv, were used as antigen and were transferred to the laboratory of leptospirosis in agricultural animals from the Museum of Microorganisms of the Institute of Veterinary Medicine of the National Academy of Sciences of Ukraine. Studies of blood serum were performed by the microagglutination test (MAT) followed by dark-field microscopy. PMA was placed in 4 dilutions: 1:50, 1: 100, 1: 500 and 1: 2500. According to numerous publications of scientists from different countries of the world, the seroprevalence level of leptospirosis infection among the dogs varies from 39% to 95%. A total of 1831 samples of blood serum were studied in the microscopic agglutination test. As a result of the serological study, 873 animals reacted positively, which is 47.7% of the total number of investigated ones. Analyzing the registered antibody titers, which is most often found titer 1: 100, which is 50.4% of the total number of positive reactions. This indicates the presence of a disease in dogs. Serrogroup Icterohaemorrhagiae is recorded in almost 50% of all positive reactions to leptospirosis and plays a major role in the etiology of the disease. It can be assumed that these dogs had contact with rats or their urine. In turn, the leading for these animals serogroup Canicola was detected in only a third of cases. Other serological groups played a minor role in the etiological structure. Summing up the aforesaid, according to the results of our work, serological prevalence of the pathogenesis of leptospirosis among dogs was determined to be 47.7%. Was detected the circulation of Leptospira`s antibodies in blood serum of these animals. The analysis of the etiological structure of leptospirosis showed that the dominant serogroups were Icterohaemorrhagiae and Canicola. Seven serogroups (Pomona, Sejroe, Grippot
{"title":"Leptospirosis of the dogs in Kyiv in 2016-2018, serological monitoring and analysis of the ethioological structure","authors":"S. Babyuk, O. Piskùn, V. Ukhovskyi, A. Piskun, L. Korniienko, T. Tsarenko","doi":"10.33245/2310-4902-2018-144-2-20-27","DOIUrl":"https://doi.org/10.33245/2310-4902-2018-144-2-20-27","url":null,"abstract":"Leptospirosis – common to humans and animals is a dangerous infectious disease that is caused by microbes - leptospires. The disease is accompanied by fever, kidney damage, liver, cardiovascular and nervous system. Leptospirosis in dogs is considered one of the most common diseases. Particularly difficult is the breed with a faulty type of body structure, such as: Neapolitan Mastino, Bulmastiff, English Bulldog, French Bulldog, Boxer, Bologna, Bloodhound, Basset Hound. The disease is most often diagnosed in dogs of hunting breeds, as a result of frequent contact with standing water, as well as in courtyard and stray dogs. Young animals and puppies get sick more often, as they do not have a stable immunity, the hemorrhagic form is more often diagnosed in older dogs.\u0000The subject was to study the distribution of leptospirosis among dogs, to establish the seropositivity level and to determine the seroprevalence of the most common of Leptospira serotypes that circulate among this species of animals.\u0000For research, an extensive diagnostic series of L. interrogans which includes 20 serovars, and blood serum from dogs that were selected in veterinary clinics in the city of Kyiv, were used as antigen and were transferred to the laboratory of leptospirosis in agricultural animals from the Museum of Microorganisms of the Institute of Veterinary Medicine of the National Academy of Sciences of Ukraine.\u0000Studies of blood serum were performed by the microagglutination test (MAT) followed by dark-field microscopy. PMA was placed in 4 dilutions: 1:50, 1: 100, 1: 500 and 1: 2500.\u0000According to numerous publications of scientists from different countries of the world, the seroprevalence level of leptospirosis infection among the dogs varies from 39% to 95%.\u0000A total of 1831 samples of blood serum were studied in the microscopic agglutination test. As a result of the serological study, 873 animals reacted positively, which is 47.7% of the total number of investigated ones.\u0000Analyzing the registered antibody titers, which is most often found titer 1: 100, which is 50.4% of the total number of positive reactions. This indicates the presence of a disease in dogs.\u0000Serrogroup Icterohaemorrhagiae is recorded in almost 50% of all positive reactions to leptospirosis and plays a major role in the etiology of the disease. It can be assumed that these dogs had contact with rats or their urine. In turn, the leading for these animals serogroup Canicola was detected in only a third of cases. Other serological groups played a minor role in the etiological structure.\u0000Summing up the aforesaid, according to the results of our work, serological prevalence of the pathogenesis of leptospirosis among dogs was determined to be 47.7%. Was detected the circulation of Leptospira`s antibodies in blood serum of these animals. The analysis of the etiological structure of leptospirosis showed that the dominant serogroups were Icterohaemorrhagiae and Canicola. Seven serogroups (Pomona, Sejroe, Grippot","PeriodicalId":34230,"journal":{"name":"Naukovii visnik veterinarnoyi meditsini","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2018-12-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"69582750","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2018-12-24DOI: 10.33245/2310-4902-2018-144-2-37-43
Y. Prykhodko, V. I. Byrka, O. Mazannyy, A. Antipov
Livestock breeding has been one of the main branches of animal husbandry in Ukraine including Slobozhanshchina (east of Ukraine). Breeding of small horned animals is the most important component of cattle breeding as the above category of ruminant animals is less whimsical to feeding and maintenance conditions. The worsening of the above conditions leads to the decrease in the animal productivity, their body resistance and the state of their immune system and it leads to re-infestation of the animals by helminths, eimeria and other zooparasites that periodically occurred on the objects of our research. Economical loss from some zooparasites and their associations in the above category of farm animals is connected with the improper feeding, decrease in the young animal preservation, retardation of their growth and development as well as with the disturbances of the reproductive function in the animals of the older age, the decrease in the quality and quantity of products and the increase in the forage cost. The publications made by the staff of the parasitology department of Kharkiv State Zooveterinary Academy and the results of the last coproscopic investigation of the animals that was carried out in autumn 2017 proved that the farm of small horned animals in the Training and Practical Complex of plant and animal husbandry in Kharkiv State Zooveterinary Academy (TPC KhSZVA) had been periodically unfavorable and there were outbreaks of invasive diseases connected with the regular breaking of the animal management, improper keeping and feeding conditions, breaking of veterinary and sanitary requirements. The research was planned and conducted in connection with the detection of zooparasite associations of different combinations in the sheep and with the appearance of veterinary preparations in the market macrolidic drug – «Ivermecvet 1 %». The aim of the investigation was to analyze the epizootic situation on the above farm in TPC KhSZVA, to practice coproscopic diagnosis of trichurosis in sheep and to develop more effective anthelminthic drug to control the above association of zooparasites and to work out more effective health measures for the above unfavorable farm and with the help of the publication we would like to share our experience to the owners of other unfavorable farms in the eastern part of Ukraine. Materials and methods. Fifty five unfavorable sheep of Precos breed from the collection herd of TPC KhSZVA were taken for the investigation. The standardized methods of coproscopy – flotation by Fulleborn and sedimentation were used for the laboratory diagnosis. By the results of the study it has been found out that the sheep in TPC KhSZVA at the beginning of the stall period of maintenance were unfavorable as for trichurosis (EI=59,3 %), strongylatosis of the digestive tract (79,6 %) and eimeriosis (57,4 %). The main pathogenic factor was trichurosis invasion that coursed simultaneously of rumen- and- intestinal strongilatosis and eimerio
{"title":"Efficacy of «Ivermecvet 1 %» for zooparasitocenoses of sheep","authors":"Y. Prykhodko, V. I. Byrka, O. Mazannyy, A. Antipov","doi":"10.33245/2310-4902-2018-144-2-37-43","DOIUrl":"https://doi.org/10.33245/2310-4902-2018-144-2-37-43","url":null,"abstract":"Livestock breeding has been one of the main branches of animal husbandry in Ukraine including Slobozhanshchina (east of Ukraine). Breeding of small horned animals is the most important component of cattle breeding as the above category of ruminant animals is less whimsical to feeding and maintenance conditions. The worsening of the above conditions leads to the decrease in the animal productivity, their body resistance and the state of their immune system and it leads to re-infestation of the animals by helminths, eimeria and other zooparasites that periodically occurred on the objects of our research. Economical loss from some zooparasites and their associations in the above category of farm animals is connected with the improper feeding, decrease in the young animal preservation, retardation of their growth and development as well as with the disturbances of the reproductive function in the animals of the older age, the decrease in the quality and quantity of products and the increase in the forage cost. The publications made by the staff of the parasitology department of Kharkiv State Zooveterinary Academy and the results of the last coproscopic investigation of the animals that was carried out in autumn 2017 proved that the farm of small horned animals in the Training and Practical Complex of plant and animal husbandry in Kharkiv State Zooveterinary Academy (TPC KhSZVA) had been periodically unfavorable and there were outbreaks of invasive diseases connected with the regular breaking of the animal management, improper keeping and feeding conditions, breaking of veterinary and sanitary requirements. The research was planned and conducted in connection with the detection of zooparasite associations of different combinations in the sheep and with the appearance of veterinary preparations in the market macrolidic drug – «Ivermecvet 1 %». The aim of the investigation was to analyze the epizootic situation on the above farm in TPC KhSZVA, to practice coproscopic diagnosis of trichurosis in sheep and to develop more effective anthelminthic drug to control the above association of zooparasites and to work out more effective health measures for the above unfavorable farm and with the help of the publication we would like to share our experience to the owners of other unfavorable farms in the eastern part of Ukraine.\u0000Materials and methods. Fifty five unfavorable sheep of Precos breed from the collection herd of TPC KhSZVA were taken for the investigation. The standardized methods of coproscopy – flotation by Fulleborn and sedimentation were used for the laboratory diagnosis. By the results of the study it has been found out that the sheep in TPC KhSZVA at the beginning of the stall period of maintenance were unfavorable as for trichurosis (EI=59,3 %), strongylatosis of the digestive tract (79,6 %) and eimeriosis (57,4 %). The main pathogenic factor was trichurosis invasion that coursed simultaneously of rumen- and- intestinal strongilatosis and eimerio","PeriodicalId":34230,"journal":{"name":"Naukovii visnik veterinarnoyi meditsini","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2018-12-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"69583278","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2018-12-24DOI: 10.33245/2310-4902-2018-144-2-66-80
L. Slivinska, I. Maksymovych
Respiratory diseases in horses are one of the main reasons for their exclusion from work, sports or recreational use. Recurrent airway obstruction (RAO), or asthma of horses – This is a disease of older horses characterized by neutrophilic inflammation of the mucous membrane, hyperactivity of the respiratory tract, hypersecretion of mucus and bronchospasm. The prevalence of broncho-pulmonary pathology, in particular asthma in horses, and low efficiency of therapeutic measures are of interest to veterinary specialists in search of new available pharmaco-correction. The purpose of the work was to study the effectiveness of the developed integrated treatment scheme for horses for asthma syndrome. Materials for research were sports and workhorses of the Ukrainian warmblood, Hanoverian, Westphalian, English Thoroughbred, Tori breeds and non-breeding animals. To complete the task, 13 horses with asthma were selected. It was established that the complex treatment of patients with asthma horses showed a positive effect, even after a short course of therapy, since the clinical recovery was manifested in reducing the frequency of cough attacks, lack of dyspnea and nasal discharge, reduction of the amount of tracheal mucus (0/1°) and neutrophils in the BAL's washings, increasing the efficiency of horses. In horses after treatment, the number of red blood cells (p 0.05) is reduced, the hemoglobin concentration (p<0.05) and the hematocrit (p<0.05) decrease, which is associated with the elimination of the causes of hypoxia, the indices of red blood are normal, as the decrease in the average volume of erythrocytes (p<0.01) and the average content of hemoglobin in erythrocyte (p<0,001) is established in comparison with the indicators of diseased animals. Conducted treatment of asthma in horses contributed to the elimination of inflammatory process in the respiratory tract, as the amount of leukocytes (p<0.01), strain-cells (p<0.01) and segmentally nuclear (p<0.01) neutrophils, as well as monocytes (p<0.05), is decreased in blood. At the same time, an increase in the number of lymphocytes in the blood of horses after treatment (p<0.001) is associated with the restoration of protective mechanisms of the body of horses. The treatment contributed to the normalization of the parameters of thrombopoiesis, in particular in the blood of horses the number of thrombocytes was likely to increase (p<0.05) and the amount of thrombocyte (p<0.05), which indicates the prevention of the development of hyperdestructive thrombocytopenia and hyperplasia of the epithelium of the respiratory tract, proliferation of smooth muscle cells and development of bronchoconstriction. Treatment of patients with asthma of horses contributed to a reduction in the blood contents of the total protein (p<0.05), which is the result of reducing the inflammatory reaction in the respiratory tract. At the same time, the content of albumins and the concentration of total bilirubin in blood of horses did n
{"title":"Treatment of horses with asthma syndrome","authors":"L. Slivinska, I. Maksymovych","doi":"10.33245/2310-4902-2018-144-2-66-80","DOIUrl":"https://doi.org/10.33245/2310-4902-2018-144-2-66-80","url":null,"abstract":"Respiratory diseases in horses are one of the main reasons for their exclusion from work, sports or recreational use. Recurrent airway obstruction (RAO), or asthma of horses – This is a disease of older horses characterized by neutrophilic inflammation of the mucous membrane, hyperactivity of the respiratory tract, hypersecretion of mucus and bronchospasm.\u0000The prevalence of broncho-pulmonary pathology, in particular asthma in horses, and low efficiency of therapeutic measures are of interest to veterinary specialists in search of new available pharmaco-correction.\u0000The purpose of the work was to study the effectiveness of the developed integrated treatment scheme for horses for asthma syndrome.\u0000Materials for research were sports and workhorses of the Ukrainian warmblood, Hanoverian, Westphalian, English Thoroughbred, Tori breeds and non-breeding animals. To complete the task, 13 horses with asthma were selected.\u0000It was established that the complex treatment of patients with asthma horses showed a positive effect, even after a short course of therapy, since the clinical recovery was manifested in reducing the frequency of cough attacks, lack of dyspnea and\u0000nasal discharge, reduction of the amount of tracheal mucus (0/1°) and neutrophils in the BAL's washings, increasing the efficiency of horses.\u0000In horses after treatment, the number of red blood cells (p 0.05) is reduced, the hemoglobin concentration (p<0.05) and the hematocrit (p<0.05) decrease, which is associated with the elimination of the causes of hypoxia, the indices of red blood\u0000are normal, as the decrease in the average volume of erythrocytes (p<0.01) and the average content of hemoglobin in erythrocyte (p<0,001) is established in comparison with the indicators of diseased animals.\u0000Conducted treatment of asthma in horses contributed to the elimination of inflammatory process in the respiratory tract, as the amount of leukocytes (p<0.01), strain-cells (p<0.01) and segmentally nuclear (p<0.01) neutrophils, as well as monocytes (p<0.05), is decreased in blood. At the same time, an increase in the number of lymphocytes in the blood of horses after treatment (p<0.001) is associated with the restoration of protective mechanisms of the body of horses.\u0000The treatment contributed to the normalization of the parameters of thrombopoiesis, in particular in the blood of horses the number of thrombocytes was likely to increase (p<0.05) and the amount of thrombocyte (p<0.05), which indicates the\u0000prevention of the development of hyperdestructive thrombocytopenia and hyperplasia of the epithelium of the respiratory tract, proliferation of smooth muscle cells and development of bronchoconstriction.\u0000Treatment of patients with asthma of horses contributed to a reduction in the blood contents of the total protein (p<0.05), which is the result of reducing the inflammatory reaction in the respiratory tract. At the same time, the content of albumins\u0000and the concentration of total bilirubin in blood of horses did n","PeriodicalId":34230,"journal":{"name":"Naukovii visnik veterinarnoyi meditsini","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2018-12-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"69582862","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2018-12-24DOI: 10.33245/2310-4902-2018-144-2-28-36
L. Korniienko
Every year, more than 55,000 people in the world die of rabies, which is confirmed by the data of the WHO expert committee. Up to 40% of victims, from bites sick or suspected of rabies animals are children under the age of 15 years. The source of the rabies agent in 99% of human deaths was dogs. More than 15 million people around the world receive anti-rabies vaccinations after contact with sick or rabies-pets. According to experts, this prevents 327 thousand deaths from rabies a year. The susceptibility to skeletal infection of various species of animals, involvement in the epizootic chain, in addition to wildlife, also in domestic and farm animals, has become an extremely high risk for humans, and the lack of means for treating rabies - determine its special place among all contagious diseases. In the current situation, a rabie must be viewed as an international rather than a local or national problem, so it describes the disease as a global scale noseau [17]. After all, according to the statistics of the International Office of Epizootics (MEB) of the governing body of the World Organization for Animal Health (WHO) cases of rabies recorded in more than 150 countries of the world. Rabies free are only the countries of Oceania and the United Kingdom, and in other countries the sporadic cases of this deadly disease are recorded. In Europe, this infection in the 50s has become epizootic. The "eradication" of the rabies virus in different countries of Europe during 2008-2015 was conducted in accordance with the developed and implemented Program, where the best results were received in Germany and Switzerland, in these countries the oral immunization of wild carnivores is used (without restrictions on funding). At the end of the last century, the epicenter of the rabies began to move from Eastern Europe to the territory of Poland (2001-2002), Croatia (2003) and then to the east - to the Russian Federation, the Republic of Belarus, Latvia and Ukraine. The conducted monitoring of the rabies epizootology have shown that the entire territory of Ukraine is a zone of stable disadvantage of this disease. The peak of epizootics in Ukraine, over the past 65 years, has fallen to 2007 (2393 cases). Since 2008 there were registered from one to two thousand cases of rabies. In 2017 there were registered 1356 cases of animals that were sick on rabies, despite the fact those more than 4.2 million anti-rabies vaccinations of domestic animals. The analysis of the situation shows that from year to year in Ukraine new natural fires of rabies are formed and new types of reservoir animals appear, which leads to an increase in the number of outbreaks of this disease. The main pledge of successful prevention of rabies around the world is the use of effective anti-rabies vaccines. Despite the regular implementation of planned antiepizootic measures in the southern region of Ukraine (including in the Skadovsk district), there is a tendency to spread the rabies. From year to
{"title":"Mоnitoring the features of the episothology of the talk in scada district of Kherson region","authors":"L. Korniienko","doi":"10.33245/2310-4902-2018-144-2-28-36","DOIUrl":"https://doi.org/10.33245/2310-4902-2018-144-2-28-36","url":null,"abstract":"Every year, more than 55,000 people in the world die of rabies, which is confirmed by the data of the WHO expert committee. Up to 40% of victims, from bites sick or suspected of rabies animals are children under the age of 15 years. The source of the rabies agent in 99% of human deaths was dogs.\u0000More than 15 million people around the world receive anti-rabies vaccinations after contact with sick or rabies-pets. According to experts, this prevents 327 thousand deaths from rabies a year.\u0000The susceptibility to skeletal infection of various species of animals, involvement in the epizootic chain, in addition to wildlife, also in domestic and farm animals, has become an extremely high risk for humans, and the lack of means for treating rabies - determine its special place among all contagious diseases.\u0000In the current situation, a rabie must be viewed as an international rather than a local or national problem, so it describes the disease as a global scale noseau [17]. After all, according to the statistics of the International Office of Epizootics (MEB) of the governing body of the World Organization for Animal Health (WHO) cases of rabies recorded in more than 150 countries of the world.\u0000Rabies free are only the countries of Oceania and the United Kingdom, and in other countries the sporadic cases of this deadly disease are recorded. In Europe, this infection in the 50s has become epizootic. The \"eradication\" of the rabies virus in different countries of Europe during 2008-2015 was conducted in accordance with the developed and implemented Program, where the best results were received in Germany and Switzerland, in these countries the oral immunization of wild carnivores is used (without restrictions on funding).\u0000At the end of the last century, the epicenter of the rabies began to move from Eastern Europe to the territory of Poland (2001-2002), Croatia (2003) and then to the east - to the Russian Federation, the Republic of Belarus, Latvia and Ukraine.\u0000The conducted monitoring of the rabies epizootology have shown that the entire territory of Ukraine is a zone of stable disadvantage of this disease. The peak of epizootics in Ukraine, over the past 65 years, has fallen to 2007 (2393 cases). Since 2008 there were registered from one to two thousand cases of rabies. In 2017 there were registered 1356 cases of animals that were sick on rabies, despite the fact those more than 4.2 million anti-rabies vaccinations of domestic animals. The analysis of the situation shows that from year to year in Ukraine new natural fires of rabies are formed and new types of reservoir animals appear, which leads to an increase in the number of outbreaks of this disease.\u0000The main pledge of successful prevention of rabies around the world is the use of effective anti-rabies vaccines. Despite the regular implementation of planned antiepizootic measures in the southern region of Ukraine (including in the Skadovsk district), there is a tendency to spread the rabies. From year to","PeriodicalId":34230,"journal":{"name":"Naukovii visnik veterinarnoyi meditsini","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2018-12-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"69583272","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2018-12-24DOI: 10.33245/2310-4902-2018-144-2-60-65
V. Sakara, A. Melnik, P. Moskalenko
Depending on age and live weight in the bird, blood can be taken in different places: by performing a puncture of the shoulder (subclavian), jugular, medial tibial veins, of the occipital sinus, of the heart, and through decapitation in the day-old young. But not all of these methods are practical and suitable for all types and periods of time in the poultry. The article describes two practical methods of life-time selection of blood in chickens-broilers of the cross-breed COBB-500 of different ages. Blood in day-old chicks is taken for the purpose of early diagnosis of deficiency of micro-and macro elements. In chickens that were hatched after 12 hours at a mass of at least 30 grams of blood, they were taken from a right jugular vein with an insulin syringe of 1 ml and then received 0,5-0,6 ml of whey individually from each chicken. Thanks to this, the chick remains alive and after 7 days it allows you to take blood again for further research. A subcutaneous vein subunit for taking blood in broiler chickens after 7 days and up to 17 days is better to use a syringe of 2 ml, as this is less injurious to the vein, but it is better to carry out this procedure with the assistant. It is more practical to make blood collection in chickens from 18 to 42 days using an injection needle with a pink cannula (18 G) and a polypropylene tube with a tufted lid that will allow it to remove up to 5 ml of blood from one bird and get enough serum for it biochemical studies. An important stage in the diagnosis of internal bird diseases, in particular metabolic etiology, is blood research. This allows us to diagnose the subclinical stages of illness associated with an imbalance of metabolic processes in an organism of productive and exotic birds. Therefore, one of the important measures in the establishment and confirmation of the diagnosis, as well as the study of the effectiveness of therapeutic treatments – is the selection of blood. Anatomical features of the body structure of various types of agricultural and exotic birds require the modification and modernization of blood selection technologies and make adjustments to the selection of the appropriate sites for manipulation. Blood in the bird collapses fast enough – 20-30 seconds, which makes it impossible to remove enough of it in young birds. In order to obtain the required volume of quality blood and its serum, it is necessary to take into account the age, physiological and productive qualities of birds. The most recent features were the development of new and improved existing methods of blood sampling in poultry. In the bird, blood can be taken by performing a puncture of the shoulder (subclavian), jugular, medial tibial veins, of the occipital sinus, of the puncture of the heart, and of the decapitation in a day-old youngster. The bird has a relatively small percentage of blood volume by weight, approximately 6-7,5%. The amount of blood that can be taken will depend on the weight of the bird, the skill of
{"title":"Features of blood selection in kurchat broilers of different age","authors":"V. Sakara, A. Melnik, P. Moskalenko","doi":"10.33245/2310-4902-2018-144-2-60-65","DOIUrl":"https://doi.org/10.33245/2310-4902-2018-144-2-60-65","url":null,"abstract":"Depending on age and live weight in the bird, blood can be taken in different places: by performing a puncture of the shoulder (subclavian), jugular, medial tibial veins, of the occipital sinus, of the heart, and through decapitation in the day-old young. But not all of these methods are practical and suitable for all types and periods of time in the poultry. The article describes two practical methods of life-time selection of blood in chickens-broilers of the cross-breed COBB-500 of different ages. Blood in day-old chicks is taken for the purpose of early diagnosis of deficiency of micro-and macro elements. In chickens that were hatched after 12 hours at a mass of at least 30 grams of blood, they were taken from a right jugular vein with an insulin syringe of 1 ml and then received 0,5-0,6 ml of whey individually from each chicken. Thanks to this, the chick remains alive and after 7 days it allows you to take blood again for further research. A subcutaneous vein subunit for taking blood in broiler chickens after 7 days and up to 17 days is better to use a syringe of 2 ml, as this is less injurious to the vein, but it is better to carry out this procedure with the assistant. It is more practical to make blood collection in chickens from 18 to 42 days using an injection needle with a pink cannula (18 G) and a polypropylene tube with a tufted lid that will allow it to remove up to 5 ml of blood from one bird and get enough serum for it biochemical studies. \u0000An important stage in the diagnosis of internal bird diseases, in particular metabolic etiology, is blood research. This allows us to diagnose the subclinical stages of illness associated with an imbalance of metabolic processes in an organism of productive and exotic birds. Therefore, one of the important measures in the establishment and confirmation of the diagnosis, as well as the study of the effectiveness of therapeutic treatments – is the selection of blood. Anatomical features of the body structure of various types of agricultural and exotic birds require the modification and modernization of blood selection technologies and make adjustments to the selection of the appropriate sites for manipulation. Blood in the bird collapses fast enough – 20-30 seconds, which makes it impossible to remove enough of it in young birds. In order to obtain the required volume of quality blood and its serum, it is necessary to take into account the age, physiological and productive qualities of birds. The most recent features were the development of new and improved existing methods of blood sampling in poultry.\u0000In the bird, blood can be taken by performing a puncture of the shoulder (subclavian), jugular, medial tibial veins, of the occipital sinus, of the puncture of the heart, and of the decapitation in a day-old youngster. The bird has a relatively small percentage of blood volume by weight, approximately 6-7,5%. The amount of blood that can be taken will depend on the weight of the bird, the skill of","PeriodicalId":34230,"journal":{"name":"Naukovii visnik veterinarnoyi meditsini","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2018-12-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"69582852","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2018-12-24DOI: 10.33245/2310-4902-2018-144-2-54-59
D. Sliusarenko, M. Ilnitsky
The paper deals with the results of investigation inflammation markers level ‒ IL-1-RA (receptor antagonist IL-1), IL-4, IL-6 cows serum, patients with soles ulcers when performing orthopedic hoof treatment with two methods ‒ local treatment, and complex method ‒ local treatment in combination with the of epidural analgesia 0,2% bupivacaine. Therapeutic blockade with local anesthetics make analgesic and pathogenetic therapeutic effects on the animal organism, can be successfully used as a component of postoperative analgesia in surgical treatment. Bupivacaine ‒ local anesthetic can be used for epidural analgesia in the postoperative period with the blocking of sensory fibers without loss of motor function. It makes sense to study using of 0,2% bupivacaine solution in clinical conditions with a therapeutic purpose in surgical diseases of cattle. Determination of the level of interleukins is informative in relation to the study of the course of the inflammation process, the immune status of animals during treatment, and indicates the intensity of regenerative processes in damaged tissues. The purpose of the research is to determine the state of inflammatory response to the level of serum blood cytokines in cows with hoof sole ulcers for two treatment methods ‒ local in animals of the control group and integrated treatment in animals of the experimental group, which included local treatment with the therapeutic bupivacaine epidural blocks. In the control group of animals (n=5), only the local treatment. Animals of the experimental group (n=5), conducted an epidural puncture and catheterization between the last sacral and the first caudal vertebrae. The catheter was left in the epidural space. Bupivacaine injection was performed with an analgesic and therapeutic purpose of 0,2 % solution in terms 3 days. After the first injection of bupivacaine, the hoof sole was cleaned and treated locally. As inflammatory markers, the level of IL-1-RA (IL-1 receptor antagonist), IL-4, IL-6 in serum was studied, since the level of these indicators reflects the state of the pro- and anti-inflammatory system. In the course of the research, the obtained results of the control and experimental groups among themselves were compared, with the baseline data before treatment in each group, as well as with the indicators of clinically healthy cows. We conducted a comparative characteristic of indicators in the application of integrated treatment compared with only local treatment of the site of the lesion. Blood was taken from the tail vein. Stages of research: before orthopedic treatment, after orthopedic treatment in 30 minutes, 3 days, 14 days, 34 days after starting treatment. Blood serum tests were conducted in the Laboratory “Alvis-class”, Kharkiv using the semi-automatic immunoassay analyzer StatFax 303+ (USA). It was found that the use of a complex treatment method is accompanied by a lower level of inflammatory reaction of the body of cows compared with only local t
关键词:足底溃疡，硬膜外镇痛，布比卡因，白细胞介素，IL-1-RA, IL-4, IL-6，牛
{"title":"Cytokine profile of cattle blood serum in the treatment of the hoof sole ulcers","authors":"D. Sliusarenko, M. Ilnitsky","doi":"10.33245/2310-4902-2018-144-2-54-59","DOIUrl":"https://doi.org/10.33245/2310-4902-2018-144-2-54-59","url":null,"abstract":"The paper deals with the results of investigation inflammation markers level ‒ IL-1-RA (receptor antagonist IL-1), IL-4, IL-6 cows serum, patients with soles ulcers when performing orthopedic hoof treatment with two methods ‒ local treatment, and complex method ‒ local treatment in combination with the of epidural analgesia 0,2% bupivacaine.\u0000Therapeutic blockade with local anesthetics make analgesic and pathogenetic therapeutic effects on the animal organism, can be successfully used as a component of postoperative analgesia in surgical treatment. Bupivacaine ‒ local anesthetic can be used for epidural analgesia in the postoperative period with the blocking of sensory fibers without loss of motor function.\u0000It makes sense to study using of 0,2% bupivacaine solution in clinical conditions with a therapeutic purpose in surgical diseases of cattle. Determination of the level of interleukins is informative in relation to the study of the course of the inflammation process, the immune status of animals during treatment, and indicates the intensity of regenerative processes in damaged tissues.\u0000The purpose of the research is to determine the state of inflammatory response to the level of serum blood cytokines in cows with hoof sole ulcers for two treatment methods ‒ local in animals of the control group and integrated treatment in animals of the experimental group, which included local treatment with the therapeutic bupivacaine epidural blocks.\u0000In the control group of animals (n=5), only the local treatment. Animals of the experimental group (n=5), conducted an epidural puncture and catheterization between the last sacral and the first caudal vertebrae. The catheter was left in the epidural space. Bupivacaine injection was performed with an analgesic and therapeutic purpose of 0,2 % solution in terms 3 days. After the first injection of bupivacaine, the hoof sole was cleaned and treated locally. As inflammatory markers, the level of IL-1-RA (IL-1 receptor antagonist), IL-4, IL-6 in serum was studied, since the level of these indicators reflects the state of the pro- and anti-inflammatory system. In the course of the research, the obtained results of the control and experimental groups among themselves were compared, with the baseline data before treatment in each group, as well as with the indicators of clinically healthy cows.\u0000We conducted a comparative characteristic of indicators in the application of integrated treatment compared with only local treatment of the site of the lesion. Blood was taken from the tail vein. Stages of research: before orthopedic treatment, after orthopedic treatment in 30 minutes, 3 days, 14 days, 34 days after starting treatment. Blood serum tests were conducted in the Laboratory “Alvis-class”, Kharkiv using the semi-automatic immunoassay analyzer StatFax 303+ (USA).\u0000It was found that the use of a complex treatment method is accompanied by a lower level of inflammatory reaction of the body of cows compared with only local t","PeriodicalId":34230,"journal":{"name":"Naukovii visnik veterinarnoyi meditsini","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2018-12-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"69583289","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2018-12-24DOI: 10.33245/2310-4902-2018-144-2-81-86
M. Matsinovich
Gastrointestinal diseases in young animals are recorded quite often, especially in industrial complexes. Diseases of this group can be up to 70-80 % of the entire internal pathology of young animals. Literary data show that in the etiology and pathogenesis of the disease in weaned piglets an allergic reaction to the components of the feed, which significantly complicates its course, may play. The development of nutritional allergies is also favored by the functional insufficiency of the glands of the digestive system of pigs during the first weeks of life, as well as the violation of their functions in various diseases of the gastrointestinal tract, which can lead to incomplete protein breakdown and accumulation of antigenic substances. The aim of the study was to study the most characteristic symptoms and blood indices during experimental reproduction of food allergies in piglets and spontaneous occurrence under production conditions. Studies were performed in 2 stages. At first, under the conditions of the clinic of the Department of Internal noninfectious Animal Diseases of the Vitebsk State Academy of Veterinary Medicine formed two groups of pigs aged 30–35 days with an average weight of 8–10 kg. In animals of the experimental group, experimental reproduction of feed allergies was carried out by abruptly changing the milk type of feeding to the concentrated one. At the second stage of the research, in the conditions of a pig complex, 200 pigs of 40- to 60-day-old patients with gastroenteritis were examined. Gastroenteritis in experimental animals was non-infectious in nature and was primarily due to the sharp weaning of animals. To detect an allergic reaction, laboratory blood tests were performed using the above methods, and also the clinical manifestations of the disease were taken into account. The clinical picture of experimental pathology at first was characterized by lethargy, reluctant eating of new food, the pigs periodically showed anxiety, which was followed by apathy. During the first three days of the experiment, intestinal meteorism was observed in piglets, which in 6 animals and 66,7 % was replaced by diarrhea, and in 33.3 % animals it was accompanied by constipation. Body temperature in all porosyat remained within the normal range. On the fourth day of the experiment, one of the animals of the experimental group fell, and according to the results of the autopsy, catarrhal-hemorrhagic were found: pa-strorenteritis, typhlitis and colitis. By the twelfth day, the functions of the gastrointestinal tract were normalized in all animals of the experimental group. In the animals of the control group during clinical observation for this period, no abnormalities were identified. On the 14th day of the experiment, an intracutaneous test was performed. As an allergen, we used a protein extract from the feed used in the experiment, containing the gliadin, albumin and globulin fractions of the gluten protein. In all the animals of the exper
{"title":"Food allergies in weaned piglets in experiment and spontaneous occurrence","authors":"M. Matsinovich","doi":"10.33245/2310-4902-2018-144-2-81-86","DOIUrl":"https://doi.org/10.33245/2310-4902-2018-144-2-81-86","url":null,"abstract":"Gastrointestinal diseases in young animals are recorded quite often, especially in industrial complexes. Diseases of this group can be up to 70-80 % of the entire internal pathology of young animals. Literary data show that in the etiology and pathogenesis of the disease in weaned piglets an allergic reaction to the components of the feed, which significantly complicates its course, may play. The development of nutritional allergies is also favored by the functional insufficiency of the glands of the digestive system of pigs during the first weeks of life, as well as the violation of their functions in various diseases of the gastrointestinal tract, which can lead to incomplete protein breakdown and accumulation of antigenic substances.\u0000The aim of the study was to study the most characteristic symptoms and blood indices during experimental reproduction of food allergies in piglets and spontaneous occurrence under production conditions. Studies were performed in 2 stages. At first, under the conditions of the clinic of the Department of Internal noninfectious Animal Diseases of the Vitebsk State Academy of Veterinary Medicine formed two groups of pigs aged 30–35 days with an average weight of 8–10 kg. In animals of the experimental group, experimental reproduction of feed allergies was carried out by abruptly changing the milk type of feeding to the concentrated one. At the second stage of the research, in the conditions of a pig complex, 200 pigs of 40- to 60-day-old patients with gastroenteritis were examined. Gastroenteritis in experimental animals was non-infectious in nature and was primarily due to the sharp weaning of animals. To detect an allergic reaction, laboratory blood tests were performed using the above methods, and also the clinical manifestations of the disease were taken into account.\u0000The clinical picture of experimental pathology at first was characterized by lethargy, reluctant eating of new food, the pigs periodically showed anxiety, which was followed by apathy. During the first three days of the experiment, intestinal meteorism was observed in piglets, which in 6 animals and 66,7 % was replaced by diarrhea, and in 33.3 % animals it was accompanied by constipation. Body temperature in all porosyat remained within the normal range. On the fourth day of the experiment, one of the animals of the experimental group fell, and according to the results of the autopsy, catarrhal-hemorrhagic were found: pa-strorenteritis, typhlitis and colitis. By the twelfth day, the functions of the gastrointestinal tract were normalized in all animals of the experimental group. In the animals of the control group during clinical observation for this period, no abnormalities were identified.\u0000On the 14th day of the experiment, an intracutaneous test was performed. As an allergen, we used a protein extract from the feed used in the experiment, containing the gliadin, albumin and globulin fractions of the gluten protein. In all the animals of the exper","PeriodicalId":34230,"journal":{"name":"Naukovii visnik veterinarnoyi meditsini","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2018-12-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48523384","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2018-12-24DOI: 10.33245/2310-4902-2018-144-2-44-53
M. Rublenko, V. Chemerovsky, V. Vlasenko, N. Ulyanchich
Bone regeneration is one of the most complex and unique types of tissue regeneration, although quite long in time, comparatively, for example, with soft tissues, but provides the complete identity of the damaged site with normal bone. The most complex fractures are fragmentation, which can be occurs within wide range - 25-60% of the total number of all fractures. In such cases, due to the loss of contact with soft tissues, the fragments lose blood supply and regeneration, which leads to different bone size defect. This condition cause limitation of the main mechanisms of bone consolidation – endoostal and intramembrane ossification. In this regard, a strategic medical treatment is the replacement of bone defect with biological or synthetic material, which creates a site for the processes of reparative osteogenesis. The most widespread combined biocompatible materials in the various combinations of β-tricalcium phosphate and hydroxyapatite ("Maxresorb®", "Perossal®", "calc-i-oss®CRYSTAL", "easy-graft®CRYSTAL"), or composite composites based on bioactive and biogenic materials: hydroxylapatite + collagen (Biostite, Collagraft, Avitene, Collola, Hapkol, Collapan, MP Composite); hydroxylapatite + tricalcium phosphate + collagen ("Hydroxyapol", "Collapolum"); hydroxylapatite + collagen + sulfated glycosaminoglycans ("Biomatrix", "Osteomatrix", "Bioimplant"). Unfortunately, in veterinary medicine osteotropic materials developed for humane medicine are used only. Recently, a separate group of biocompatible composites based on the combination of hydroxyapatite with β-tricalcium phosphate, doped with magnesium, sodium, potassium, zinc, copper, aluminum, strontium, silicon, germanium, in order to provide them with specific properties - antibacterial, osteoinductive, antitumor, immunomodulating, etc. However, the spectrum of biological effects of these ions on bone metabolism is extremely diverse, and therefore the use of composite ceramics doped with microelement ions requires a comprehensive clinical and experimental justification. The purpose of the study is to evaluate the osteointegration and osteoinductive properties of ceramics based on hydroxyapatite and β-tricalcium phosphate doped with silicon for model fractures of the femur in rabbits. The work is done on rabbits of Californian breed at the age of 3 months. and a weight of about 2.5 kg. To substantiate the ceramics GTlKg-2, 2 groups of 10 rabbits were formed in each, in which model bone defects were formed in the distal parts of the hip dysthymia. Animals of the experimental group defects filled with granules of ceramics. In the rabbits of the control group, the defect was left to heal under a blood clot. Animals were extracted from the experiment at the 21st and 42nd day. X-ray and histomorphological studies were performed. On the 21st day of reparative osteogenesis, rabbits of all groups fully rested on the injured limb, signs of inflammatory reaction were absent in the experimental group, and
{"title":"Evaluation of osteointegrative and osteoinductive properties of silicon doped ceramics in a model of rabbit’s femur fractures","authors":"M. Rublenko, V. Chemerovsky, V. Vlasenko, N. Ulyanchich","doi":"10.33245/2310-4902-2018-144-2-44-53","DOIUrl":"https://doi.org/10.33245/2310-4902-2018-144-2-44-53","url":null,"abstract":"Bone regeneration is one of the most complex and unique types of tissue regeneration, although quite long in time, comparatively, for example, with soft tissues, but provides the complete identity of the damaged site with normal bone. The most complex fractures are fragmentation, which can be occurs within wide range - 25-60% of the total number of all fractures. In such cases, due to the loss of contact with soft tissues, the fragments lose blood supply and regeneration, which leads to different bone size defect. This condition cause limitation of the main mechanisms of bone consolidation – endoostal and intramembrane ossification. In this regard, a strategic medical treatment is the replacement of bone defect with biological or synthetic material, which creates a site for the processes of reparative osteogenesis.\u0000The most widespread combined biocompatible materials in the various combinations of β-tricalcium phosphate and hydroxyapatite (\"Maxresorb®\", \"Perossal®\", \"calc-i-oss®CRYSTAL\", \"easy-graft®CRYSTAL\"), or composite composites based on bioactive and biogenic materials: hydroxylapatite + collagen (Biostite, Collagraft, Avitene, Collola, Hapkol, Collapan, MP Composite); hydroxylapatite + tricalcium phosphate + collagen (\"Hydroxyapol\", \"Collapolum\"); hydroxylapatite + collagen + sulfated glycosaminoglycans (\"Biomatrix\", \"Osteomatrix\", \"Bioimplant\").\u0000Unfortunately, in veterinary medicine osteotropic materials developed for humane medicine are used only. Recently, a separate group of biocompatible composites based on the combination of hydroxyapatite with β-tricalcium phosphate, doped with magnesium, sodium, potassium, zinc, copper, aluminum, strontium, silicon, germanium, in order to provide them with specific properties - antibacterial, osteoinductive, antitumor, immunomodulating, etc. However, the spectrum of biological effects of these ions on bone metabolism is extremely diverse, and therefore the use of composite ceramics doped with microelement ions requires a comprehensive clinical and experimental justification.\u0000The purpose of the study is to evaluate the osteointegration and osteoinductive properties of ceramics based on hydroxyapatite and β-tricalcium phosphate doped with silicon for model fractures of the femur in rabbits.\u0000The work is done on rabbits of Californian breed at the age of 3 months. and a weight of about 2.5 kg. To substantiate the ceramics GTlKg-2, 2 groups of 10 rabbits were formed in each, in which model bone defects were formed in the distal parts of the hip dysthymia. Animals of the experimental group defects filled with granules of ceramics. In the rabbits of the control group, the defect was left to heal under a blood clot. Animals were extracted from the experiment at the 21st and 42nd day. X-ray and histomorphological studies were performed.\u0000On the 21st day of reparative osteogenesis, rabbits of all groups fully rested on the injured limb, signs of inflammatory reaction were absent in the experimental group, and","PeriodicalId":34230,"journal":{"name":"Naukovii visnik veterinarnoyi meditsini","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2018-12-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"69583284","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2018-12-24DOI: 10.33245/2310-4902-2018-144-2-87-99
V. Lyasota, L. Sokolova
In Ukraine there is a complicated epizootic situation caused by the spread of serious viral diseases, in particular in the pig breeding of African swine fever (ACS) and epidemic swine diarrhea (EDS). In such conditions, the market for disinfectants is actively developing, but often drugs do not prevent the spread of viral diseases. This is due to the discrepancy in methods and doses of application, the weak effect on certain viruses and bacteria, and also the wrong organization of disinfection. The veterinary well-being of livestock farms, complexes and poultry farms largely depends on the regular and thorough carrying out of veterinary and sanitary measures. Disinfection is an important part of the measures aimed at preventing and controlling infectious animal diseases. In most cases, existing disinfectants and recommendations for their use were designed for large commodity and industrial complexes that do not fully meet the requirements of small farms. Most commonly used disposables are toxic to humans and animals (sodium or potassium hydroxide solution, bleach, phenol and others), so they should be carefully used to prevent poisoning. In veterinary practice there are practically no ecologically clean and safe disinfection means that can be used for sanitation of various objects of veterinary supervision, including in the presence of animals and poultry. The practice of using disinfectants in agriculture for persistent chemicals such as bleach, hydrogen peroxide, formaldehyde and several others have proved to be unserviceable in many ways. Before all it is biological harmfulness, impossibility to carry out disinfection in the presence of animals and poultry, adaptation of pathogenic microflora, high cost, high complexity of treatment of objects, clogging of the external environment, etc. Most modern low-toxic disinfectants are used in the form of solutions by irrigation or aerosols, but it is not possible to rehabilitate their premises in the presence of animals. The use of these agents is also relatively labor-intensive, greatly increases the humidity in the room, and there is a likelihood of accumulation of their residual amounts in meat. Therefore, as an option - it is worth considering the current disinfection of premises with dry biocidal preparations, for example: Stalosan F (Vitfoss, Denmark), Dezosan Vigor (JHJ, Poland), Advais draj (NutriConcept, France), Lyubisan-eko, Lyubisan pyglet (LLC Ekodisan-Ukraine), Clinosan "ZVK" (Ukraine), Mecadzade (NPC "Globus", Ukraine) and others. These disinfectants, in their properties, are environmentally friendly, represent an amorphous powder of pleasant smell, which can absorb moisture. They are effective in the destruction and control of many bacteria, viruses, fungi, parasites, and fly larvae. In addition, they improve the quality of the litter, reduce the ammonia content and moisture in livestock buildings. The main properties of the above-mentioned disinfectants include: use without restriction
{"title":"Disinfectants, modern characteristics and safety of use in animal husbandry","authors":"V. Lyasota, L. Sokolova","doi":"10.33245/2310-4902-2018-144-2-87-99","DOIUrl":"https://doi.org/10.33245/2310-4902-2018-144-2-87-99","url":null,"abstract":"In Ukraine there is a complicated epizootic situation caused by the spread of serious viral diseases, in particular in the pig breeding of African swine fever (ACS) and epidemic swine diarrhea (EDS). In such conditions, the market for disinfectants is actively developing, but often drugs do not prevent the spread of viral diseases. This is due to the discrepancy in methods and doses of application, the weak effect on certain viruses and bacteria, and also the wrong organization of disinfection. The veterinary well-being of livestock farms, complexes and poultry farms largely depends on the regular and thorough carrying out of veterinary and sanitary measures.\u0000Disinfection is an important part of the measures aimed at preventing and controlling infectious animal diseases. In most cases, existing disinfectants and recommendations for their use were designed for large commodity and industrial complexes that do not fully meet the requirements of small farms. Most commonly used disposables are toxic to humans and animals (sodium or potassium hydroxide solution, bleach, phenol and others), so they should be carefully used to prevent poisoning.\u0000In veterinary practice there are practically no ecologically clean and safe disinfection means that can be used for sanitation of various objects of veterinary supervision, including in the presence of animals and poultry. The practice of using disinfectants in agriculture for persistent chemicals such as bleach, hydrogen peroxide, formaldehyde and several others have proved to be unserviceable in many ways.\u0000Before all it is biological harmfulness, impossibility to carry out disinfection in the presence of animals and poultry, adaptation of pathogenic microflora, high cost, high complexity of treatment of objects, clogging of the external environment, etc.\u0000Most modern low-toxic disinfectants are used in the form of solutions by irrigation or aerosols, but it is not possible to rehabilitate their premises in the presence of animals. The use of these agents is also relatively labor-intensive, greatly increases the humidity in the room, and there is a likelihood of accumulation of their residual amounts in meat.\u0000Therefore, as an option - it is worth considering the current disinfection of premises with dry biocidal preparations, for example: Stalosan F (Vitfoss, Denmark), Dezosan Vigor (JHJ, Poland), Advais draj (NutriConcept, France), Lyubisan-eko, Lyubisan pyglet (LLC Ekodisan-Ukraine), Clinosan \"ZVK\" (Ukraine), Mecadzade (NPC \"Globus\", Ukraine) and others.\u0000These disinfectants, in their properties, are environmentally friendly, represent an amorphous powder of pleasant smell, which can absorb moisture.\u0000They are effective in the destruction and control of many bacteria, viruses, fungi, parasites, and fly larvae. In addition, they improve the quality of the litter, reduce the ammonia content and moisture in livestock buildings.\u0000The main properties of the above-mentioned disinfectants include: use without restriction","PeriodicalId":34230,"journal":{"name":"Naukovii visnik veterinarnoyi meditsini","volume":"102 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2018-12-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"69582919","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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