iScience最新文献

英文中文

Age-dependent immune responses and resident cell dynamics in young mice following pneumonia 肺炎后年轻小鼠的年龄依赖性免疫反应和常驻细胞动力学

IF 4.1 2区综合性期刊 Q1 MULTIDISCIPLINARY SCIENCES

iScience

Pub Date : 2025-11-26 DOI: 10.1016/j.isci.2025.114219

Sylvia N. Michki , Javier V. Perez , Dharma A. Varapula , Aaron J. Thomas , Tiffaney L. Vincent , Benjamin D. Singer , Jarod A. Zepp , Sharon A. McGrath-Morrow

Infancy represents a unique period of immune vulnerability. This study investigated age-specific immune dynamics associated with differential health outcomes in the immature lung. Single-cell RNA sequencing (scRNA-seq) and bulk RNA sequencing were employed to comprehensively map the transcriptional landscapes of lung-resident cells. Marked differences in gene expression and cell-type distributions were observed between neonates and juveniles. Acute inflammatory responses in neonatal lungs were associated with decreased alveolar epithelial type 2 (AT2) cell proliferation, as well as a transient disruption of ligand-receptor interactions (Fgf1 and Fgfr4) between AT2 cells and secondary crest myofibroblasts. In contrast, juvenile immune and lung-resident cells were transcriptionally poised to respond to lung injury, dampening the acute inflammatory effects of E. coli on the lung. This study highlighted how early-life immune ontogeny impacts disease susceptibility, opening avenues for future research to identify therapeutic targets to enhance resistance to respiratory infections for the most vulnerable populations.

婴儿期是免疫系统脆弱的独特时期。本研究调查了未成熟肺中与不同健康结果相关的年龄特异性免疫动力学。单细胞RNA测序（scRNA-seq）和大量RNA测序被用于全面绘制肺驻留细胞的转录景观。幼鱼和幼鱼在基因表达和细胞类型分布上存在显著差异。新生儿肺部的急性炎症反应与肺泡上皮2型（AT2）细胞增殖减少以及AT2细胞与继发性嵴肌成纤维细胞之间配体-受体相互作用（Fgf1和Fgfr4）的短暂中断有关。相反，幼年免疫细胞和肺驻留细胞在转录上准备对肺损伤做出反应，抑制大肠杆菌对肺的急性炎症作用。这项研究强调了生命早期免疫个体发生如何影响疾病易感性，为未来的研究开辟了道路，以确定治疗靶点，增强最脆弱人群对呼吸道感染的抵抗力。

{"title":"Age-dependent immune responses and resident cell dynamics in young mice following pneumonia","authors":"Sylvia N. Michki , Javier V. Perez , Dharma A. Varapula , Aaron J. Thomas , Tiffaney L. Vincent , Benjamin D. Singer , Jarod A. Zepp , Sharon A. McGrath-Morrow","doi":"10.1016/j.isci.2025.114219","DOIUrl":"10.1016/j.isci.2025.114219","url":null,"abstract":"<div><div>Infancy represents a unique period of immune vulnerability. This study investigated age-specific immune dynamics associated with differential health outcomes in the immature lung. Single-cell RNA sequencing (scRNA-seq) and bulk RNA sequencing were employed to comprehensively map the transcriptional landscapes of lung-resident cells. Marked differences in gene expression and cell-type distributions were observed between neonates and juveniles. Acute inflammatory responses in neonatal lungs were associated with decreased alveolar epithelial type 2 (AT2) cell proliferation, as well as a transient disruption of ligand-receptor interactions (<em>Fgf1 and Fgfr4</em>) between AT2 cells and secondary crest myofibroblasts. In contrast, juvenile immune and lung-resident cells were transcriptionally poised to respond to lung injury, dampening the acute inflammatory effects of <em>E</em>. <em>coli</em> on the lung. This study highlighted how early-life immune ontogeny impacts disease susceptibility, opening avenues for future research to identify therapeutic targets to enhance resistance to respiratory infections for the most vulnerable populations.</div></div>","PeriodicalId":342,"journal":{"name":"iScience","volume":"29 1","pages":"Article 114219"},"PeriodicalIF":4.1,"publicationDate":"2025-11-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145735585","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

An SFN-YOLO-based detection method for the fermentation stage in the anaerobic digestion process 一种基于sfn - yolo的厌氧消化发酵阶段检测方法

IF 4.1 2区综合性期刊 Q1 MULTIDISCIPLINARY SCIENCES

iScience

Pub Date : 2025-11-26 DOI: 10.1016/j.isci.2025.114243

Peng Liu , Shengxian Cao , Gong Wang , Siyuan Fan , Tianyi Sun , An Yan

Anaerobic digestion is an effective and low-carbon approach for biomass utilization and energy recovery. To accurately determine fermentation stages and enhance process control, this paper proposes an anaerobic digestion fermentation stage detection method based on SFN-YOLO. This model combines spatial feature interaction fusion pyramid network (SFIFPN), a focused linear attention (FLAtt) mechanism that concentrates on key features, and a normalized Wasserstein distance (NWD) loss for robust bounding box regression to accurately detect and classify anaerobic digestion images at different stages. The model demonstrates good detection ability on the validation dataset (precision reaches 91.9%, recall reaches 77.6%, and [email protected] reaches 81.1%), with the total proportion of correctly detected boxes reaching as high as 84.27%. This work demonstrates the potential of intelligent visual monitoring to improve the accuracy, efficiency, and automation of anaerobic digestion processes, contributing to sustainable bioenergy management.

厌氧消化是一种有效的低碳生物质利用和能量回收方法。为了准确确定发酵阶段，加强过程控制，本文提出了一种基于SFN-YOLO的厌氧消化发酵阶段检测方法。该模型结合了空间特征相互作用融合金字塔网络（SFIFPN）、集中关键特征的聚焦线性注意力（FLAtt）机制和鲁棒边界盒回归的归一化Wasserstein距离（NWD）损失，以准确检测和分类不同阶段的厌氧消化图像。该模型在验证数据集上表现出良好的检测能力（准确率达到91.9%，召回率达到77.6%，[email protected]达到81.1%），正确检测出的盒子总比例高达84.27%。这项工作证明了智能视觉监测在提高厌氧消化过程的准确性、效率和自动化方面的潜力，有助于可持续的生物能源管理。

引用次数: 0

The unanticipated role of the glial-associated glucose-6-phosphatase system in brain homeostasis 胶质相关葡萄糖-6-磷酸酶系统在脑内稳态中的意外作用

IF 4.1 2区综合性期刊 Q1 MULTIDISCIPLINARY SCIENCES

iScience

Pub Date : 2025-11-26 DOI: 10.1016/j.isci.2025.114235

María José Barahona , Katterine Salazar , Francisco Nualart

The glucose-6-phosphatase (G6Pase) system is a multiprotein complex within the endoplasmic reticulum that enables glucose export during high energy demand. Although traditionally studied in peripheral tissues such as the liver, recent studies highlight its relevance in the central nervous system, particularly in astrocytes and specialized glial cells called tanycytes. This review explores emerging functions of the G6Pase system in cerebral glial cells and its putative role in the regulation of brain bioenergetics and energy balance. In astrocytes, G6Pase expression supports glucose uptake, ATP production, and endoplasmic reticulum Ca²⁺ accumulation, contributing to cognitive processes and the counterregulatory response to hypoglycemia. In tanycytes, G6Pase is essential for promoting food intake and maintaining body weight. Collectively, these findings emphasize the importance of the G6Pase system in sustaining brain homeostasis.

葡萄糖-6-磷酸酶（G6Pase）系统是内质网内的多蛋白复合物，在高能量需求时使葡萄糖输出。虽然传统上研究的是外周组织，如肝脏，但最近的研究强调了它在中枢神经系统中的相关性，特别是在星形胶质细胞和称为谭细胞的特殊胶质细胞中。本文综述了G6Pase系统在脑胶质细胞中的新功能及其在脑生物能量学和能量平衡调节中的可能作用。在星形胶质细胞中，G6Pase表达支持葡萄糖摄取、ATP产生和内质网Ca2+积累，有助于认知过程和对低血糖的反调节反应。在伸长细胞中，G6Pase对促进食物摄入和维持体重至关重要。总的来说，这些发现强调了G6Pase系统在维持大脑稳态中的重要性。

引用次数: 0

Vibration tailored to jawbone density with near-infrared light expedites orthodontic tooth movement 振动量身定制的颚骨密度与近红外光加速正畸牙齿的运动

IF 4.1 2区综合性期刊 Q1 MULTIDISCIPLINARY SCIENCES

iScience

Pub Date : 2025-11-26 DOI: 10.1016/j.isci.2025.114237

Jinfeng Peng , Xinyuan Wang , Wencheng Song , Yaxin Wang , Lu Ye , Liang Qiao , Guangjin Chen , Lili Chen

Malocclusion, a prevalent oral health issue, requires prolonged orthodontic treatment due to inefficiency of orthodontic tooth movement (OTM). Non-invasive physical stimulation has been increasingly applied to accelerate OTM. This study investigated how age-related variations in jawbone density affect the efficacy of vibration and near-infrared (NIR) light in accelerating OTM. Human computed tomography (CT) data and rat models revealed higher jawbone density in young adults than in adolescents. A ten-channel vibration-light integrated platform (VLIP) was developed to precisely control the stimulation parameters for OTM acceleration. The results demonstrated that low-frequency vibration (L-Vib) accelerated OTM more efficiently in adolescents with lower jawbone density, whereas high-frequency vibration (H-Vib) was superior in young adults with denser jawbones. Notably, vibration and NIR light acted synergistically in accelerating OTM with long-term safety and efficacy. Therefore, the study provides a theoretical basis for developing personalized physical stimulation strategies based on jawbone density to improve orthodontic treatment efficiency.

错牙合是一种常见的口腔健康问题，由于正畸牙齿运动（OTM）效率低下，需要长期的正畸治疗。非侵入性物理刺激已越来越多地应用于加速OTM。本研究探讨了年龄相关的颌骨密度变化如何影响振动和近红外（NIR）光加速OTM的功效。人体计算机断层扫描（CT）数据和大鼠模型显示，年轻人的颌骨密度高于青少年。为了精确控制OTM加速的激励参数，开发了一个十通道振动光集成平台（VLIP）。结果表明，低频振动（L-Vib）在颌骨密度较低的青少年中更有效地加速OTM，而高频振动（H-Vib）在颌骨密度较高的青壮年中更有效。值得注意的是，振动和近红外光协同作用，加速了OTM的长期安全性和有效性。因此，本研究为制定基于颌骨密度的个性化物理刺激策略以提高正畸治疗效率提供了理论依据。

{"title":"Vibration tailored to jawbone density with near-infrared light expedites orthodontic tooth movement","authors":"Jinfeng Peng , Xinyuan Wang , Wencheng Song , Yaxin Wang , Lu Ye , Liang Qiao , Guangjin Chen , Lili Chen","doi":"10.1016/j.isci.2025.114237","DOIUrl":"10.1016/j.isci.2025.114237","url":null,"abstract":"<div><div>Malocclusion, a prevalent oral health issue, requires prolonged orthodontic treatment due to inefficiency of orthodontic tooth movement (OTM). Non-invasive physical stimulation has been increasingly applied to accelerate OTM. This study investigated how age-related variations in jawbone density affect the efficacy of vibration and near-infrared (NIR) light in accelerating OTM. Human computed tomography (CT) data and rat models revealed higher jawbone density in young adults than in adolescents. A ten-channel vibration-light integrated platform (VLIP) was developed to precisely control the stimulation parameters for OTM acceleration. The results demonstrated that low-frequency vibration (L-Vib) accelerated OTM more efficiently in adolescents with lower jawbone density, whereas high-frequency vibration (H-Vib) was superior in young adults with denser jawbones. Notably, vibration and NIR light acted synergistically in accelerating OTM with long-term safety and efficacy. Therefore, the study provides a theoretical basis for developing personalized physical stimulation strategies based on jawbone density to improve orthodontic treatment efficiency.</div></div>","PeriodicalId":342,"journal":{"name":"iScience","volume":"29 1","pages":"Article 114237"},"PeriodicalIF":4.1,"publicationDate":"2025-11-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145735574","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Battery SOH estimation based on thermodynamic parameters from an electrochemical fractional-order model and LSTM 基于电化学分数阶模型和LSTM热力学参数的电池SOH估计

IF 4.1 2区综合性期刊 Q1 MULTIDISCIPLINARY SCIENCES

iScience

Pub Date : 2025-11-26 DOI: 10.1016/j.isci.2025.114234

Jing V. Wang , Yi Liu , Qian Wang , Jianqiang Kang , Peipei Meng , Guorong Zhu

Accurate state of health (SOH) estimation is vital for lithium-ion battery safety and performance. Since SOH cannot be directly measured, traditional methods rely on external features or models but often suffer from low interpretability and complex modeling. This study proposes a physics-inspired deep learning method for SOH estimation, which combines battery mechanism models with deep learning to effectively incorporate physical insights and improve predictive performance. Thermodynamic parameters from an electrochemical fractional-order model are fed into a long short-term memory (LSTM) network to map features to SOH, enabling high-precision estimation. Compared with four other machine learning methods, LSTM achieved the best performance, with an average root-mean-square error of 0.70% and a minimum error of 0.21% across eight validation batteries. The use of thermodynamic parameters improved estimation accuracy by 3.36 times compared to traditional features like incremental capacity curves and ohmic resistance. This approach integrates physical modeling with data-driven methods, enabling high-precision battery management.

准确的健康状态（SOH）估计对于锂离子电池的安全和性能至关重要。由于SOH不能直接测量，传统方法依赖于外部特征或模型，但往往存在可解释性低和建模复杂的问题。本研究提出了一种物理启发的SOH估计深度学习方法，该方法将电池机制模型与深度学习相结合，有效地结合物理见解并提高预测性能。电化学分数阶模型的热力学参数被输入到一个长短期记忆（LSTM）网络中，将特征映射到SOH，从而实现高精度估计。与其他四种机器学习方法相比，LSTM取得了最好的性能，8组验证的平均均方根误差为0.70%，最小误差为0.21%。与增量容量曲线和欧姆电阻等传统特征相比，热力学参数的使用将估计精度提高了3.36倍。这种方法将物理建模与数据驱动方法相结合，实现了高精度的电池管理。

{"title":"Battery SOH estimation based on thermodynamic parameters from an electrochemical fractional-order model and LSTM","authors":"Jing V. Wang , Yi Liu , Qian Wang , Jianqiang Kang , Peipei Meng , Guorong Zhu","doi":"10.1016/j.isci.2025.114234","DOIUrl":"10.1016/j.isci.2025.114234","url":null,"abstract":"<div><div>Accurate state of health (SOH) estimation is vital for lithium-ion battery safety and performance. Since SOH cannot be directly measured, traditional methods rely on external features or models but often suffer from low interpretability and complex modeling. This study proposes a physics-inspired deep learning method for SOH estimation, which combines battery mechanism models with deep learning to effectively incorporate physical insights and improve predictive performance. Thermodynamic parameters from an electrochemical fractional-order model are fed into a long short-term memory (LSTM) network to map features to SOH, enabling high-precision estimation. Compared with four other machine learning methods, LSTM achieved the best performance, with an average root-mean-square error of 0.70% and a minimum error of 0.21% across eight validation batteries. The use of thermodynamic parameters improved estimation accuracy by 3.36 times compared to traditional features like incremental capacity curves and ohmic resistance. This approach integrates physical modeling with data-driven methods, enabling high-precision battery management.</div></div>","PeriodicalId":342,"journal":{"name":"iScience","volume":"29 1","pages":"Article 114234"},"PeriodicalIF":4.1,"publicationDate":"2025-11-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145712197","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

High-grade serous ovarian cancer induced in different sites of origin in mice exemplifies diverse features of the human disease 小鼠不同起源部位诱导的高级别浆液性卵巢癌体现了人类疾病的不同特征

IF 4.1 2区综合性期刊 Q1 MULTIDISCIPLINARY SCIENCES

iScience

Pub Date : 2025-11-26 DOI: 10.1016/j.isci.2025.114238

Ludmila Szabova , Lucy Lu , Melanie B. Gordon , Kristine Johnson , Theresa M. Guerin , Laura Bassel , Deborah B. Householder , Margalie Edouard , Amy Ries , Serguei Kozlov , N. Keith Collins , Wendi Custer , Kathleen Cho , Goli Samimi , Robert H. Shoemaker , Zoe Weaver Ohler

Preclinical mouse models for ovarian cancer that faithfully recapitulate human disease on both histopathological and molecular levels are crucial for advancing novel interventions into the clinic. We developed three genetically engineered mouse (GEM) models for fallopian tube-originating ovarian cancer with the loss of Brca1, Trp53, and Rb expression driven by Pax8 or Ovgp1 promoters or virally induced directly in the oviductal epithelium. We profiled the tumors by histology and gene expression and compared them to a previously described ovarian cancer model derived from ovarian surface epithelium. Expression profiles from the oviductal and ovarian epithelium tumors fall within the four subtypes of human high-grade serous carcinoma (HGSC) and represent different patient subpopulations. Allograft tumor models derived from the GEMs are amenable to preclinical intervention studies and respond to standard of care therapies. These well-defined, tractable models present a valuable resource for assessing novel drugs and immunotherapies for patients with HGSC.

卵巢癌的临床前小鼠模型在组织病理学和分子水平上忠实地概括了人类疾病，这对于推进新的干预措施进入临床至关重要。我们开发了三种用于输卵管源性卵巢癌的基因工程小鼠（GEM）模型，这些模型由Pax8或Ovgp1启动子驱动或直接在输卵管上皮中病毒诱导Brca1、Trp53和Rb表达缺失。我们通过组织学和基因表达对肿瘤进行了分析，并将其与先前描述的来自卵巢表面上皮的卵巢癌模型进行了比较。输卵管和卵巢上皮肿瘤的表达谱属于人类高级别浆液性癌（HGSC）的四个亚型，代表不同的患者亚群。来自GEMs的同种异体移植肿瘤模型适用于临床前干预研究，并对标准护理疗法有反应。这些定义良好，易于处理的模型为评估HGSC患者的新药和免疫疗法提供了宝贵的资源。

{"title":"High-grade serous ovarian cancer induced in different sites of origin in mice exemplifies diverse features of the human disease","authors":"Ludmila Szabova , Lucy Lu , Melanie B. Gordon , Kristine Johnson , Theresa M. Guerin , Laura Bassel , Deborah B. Householder , Margalie Edouard , Amy Ries , Serguei Kozlov , N. Keith Collins , Wendi Custer , Kathleen Cho , Goli Samimi , Robert H. Shoemaker , Zoe Weaver Ohler","doi":"10.1016/j.isci.2025.114238","DOIUrl":"10.1016/j.isci.2025.114238","url":null,"abstract":"<div><div>Preclinical mouse models for ovarian cancer that faithfully recapitulate human disease on both histopathological and molecular levels are crucial for advancing novel interventions into the clinic. We developed three genetically engineered mouse (GEM) models for fallopian tube-originating ovarian cancer with the loss of Brca1, Trp53, and Rb expression driven by <em>Pax8</em> or <em>Ovgp1</em> promoters or virally induced directly in the oviductal epithelium. We profiled the tumors by histology and gene expression and compared them to a previously described ovarian cancer model derived from ovarian surface epithelium. Expression profiles from the oviductal and ovarian epithelium tumors fall within the four subtypes of human high-grade serous carcinoma (HGSC) and represent different patient subpopulations. Allograft tumor models derived from the GEMs are amenable to preclinical intervention studies and respond to standard of care therapies. These well-defined, tractable models present a valuable resource for assessing novel drugs and immunotherapies for patients with HGSC.</div></div>","PeriodicalId":342,"journal":{"name":"iScience","volume":"29 1","pages":"Article 114238"},"PeriodicalIF":4.1,"publicationDate":"2025-11-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145735583","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Fine-tuning thermogenesis from the post-transcriptional level 从转录后水平微调产热作用

IF 4.1 2区综合性期刊 Q1 MULTIDISCIPLINARY SCIENCES

iScience

Pub Date : 2025-11-26 DOI: 10.1016/j.isci.2025.114239

Mengyao Wan , Hao Gu , Siyuan Chen , Zihao Guo , Bayindala Xiagedeer , Yaqun Guan , Xiaodi Liang

In mammals, adipose tissue is involved in energy homeostasis regulation. White adipose tissue (WAT) is the major energy storage site for mammals, whereas brown adipose tissue (BAT) is involved in energy expenditure and thermogenesis. The conversion of triglycerides from WAT into fuel provides calories for the body and aids in reducing WAT accumulation, which is considered an effective strategy for weight loss and improving lipid metabolism. Regulation at the transcriptional level can determine the differentiation and transdifferentiation of WAT and BAT; however, post-transcriptional regulation offers an additional level of coordination to this process. Despite the high number of studies investigating the transcriptional regulatory mechanisms of key thermogenesis-regulated genes, the regulatory roles of these genes at the post-transcriptional level remain poorly understood. Therefore, this review summarizes the post-transcriptional regulatory mechanisms of key thermogenesis-regulated genes, including splicing, m6A modification, RNA degradation, RNA-binding proteins, and ncRNA regulation of pre-mRNAs of these thermogenic genes.

在哺乳动物中，脂肪组织参与能量稳态调节。白色脂肪组织（WAT）是哺乳动物主要的能量储存部位，而棕色脂肪组织（BAT）则参与能量消耗和产热。从WAT转化为燃料的甘油三酯为身体提供热量，并有助于减少WAT的积累，这被认为是减肥和改善脂质代谢的有效策略。转录水平的调控可以决定WAT和BAT的分化和转分化；然而，转录后调控为这一过程提供了额外的协调水平。尽管大量研究调查了关键生热调控基因的转录调控机制，但这些基因在转录后水平的调控作用仍然知之甚少。因此，本文综述了主要产热基因的转录后调控机制，包括剪接、m6A修饰、RNA降解、RNA结合蛋白以及ncRNA对这些产热基因pre- mrna的调控。

{"title":"Fine-tuning thermogenesis from the post-transcriptional level","authors":"Mengyao Wan , Hao Gu , Siyuan Chen , Zihao Guo , Bayindala Xiagedeer , Yaqun Guan , Xiaodi Liang","doi":"10.1016/j.isci.2025.114239","DOIUrl":"10.1016/j.isci.2025.114239","url":null,"abstract":"<div><div>In mammals, adipose tissue is involved in energy homeostasis regulation. White adipose tissue (WAT) is the major energy storage site for mammals, whereas brown adipose tissue (BAT) is involved in energy expenditure and thermogenesis. The conversion of triglycerides from WAT into fuel provides calories for the body and aids in reducing WAT accumulation, which is considered an effective strategy for weight loss and improving lipid metabolism. Regulation at the transcriptional level can determine the differentiation and transdifferentiation of WAT and BAT; however, post-transcriptional regulation offers an additional level of coordination to this process. Despite the high number of studies investigating the transcriptional regulatory mechanisms of key thermogenesis-regulated genes, the regulatory roles of these genes at the post-transcriptional level remain poorly understood. Therefore, this review summarizes the post-transcriptional regulatory mechanisms of key thermogenesis-regulated genes, including splicing, m6A modification, RNA degradation, RNA-binding proteins, and ncRNA regulation of pre-mRNAs of these thermogenic genes.</div></div>","PeriodicalId":342,"journal":{"name":"iScience","volume":"28 12","pages":"Article 114239"},"PeriodicalIF":4.1,"publicationDate":"2025-11-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145733273","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Advances in the clinical application of bispecific antibodies in cancer therapy 双特异性抗体在肿瘤治疗中的临床应用进展

IF 4.1 2区综合性期刊 Q1 MULTIDISCIPLINARY SCIENCES

iScience

Pub Date : 2025-11-25 DOI: 10.1016/j.isci.2025.114203

Shiqi Zhou , Feng Li , Mengke Niu , Kongming Wu , Tianye Li , Ming Yi

Cancer immunotherapy has emerged as one of the most groundbreaking advancements. However, the tumor microenvironment (TME) is often dominated by various immunosuppressive factors, compromising the efficacy of single-target therapies, leading to non-responsiveness or resistance. Bispecific antibodies (BsAbs) represent an innovative immunotherapeutic strategy with enormous potential for improving cancer treatment outcomes. Unlike monoclonal antibodies, BsAbs can concurrently inhibit multiple pro-tumor pathways, target immune checkpoints to mitigate resistance, and bind to two distinct antigens, thereby enhancing specificity while minimizing off-target effects. Moreover, BsAbs are more cost-efficient and less toxic compared to the use of two separate monoclonal antibodies in combination. In recent decades, BsAbs have made remarkable progress in clinical development. Several BsAbs, such as blinatumomab, mosunetuzumab, teclistamab, glofitamab, epcoritamab, talquetamab, ivonescimab, cadonilimab, tarlatamab, zenocutuzumab, and catumaxomab, have achieved notable success in clinical trials. This review highlights clinically approved BsAbs and provides a comprehensive summary of their therapeutic applications in cancer treatment.

癌症免疫疗法已经成为最具突破性的进步之一。然而，肿瘤微环境（TME）往往由多种免疫抑制因子主导，影响单靶点治疗的疗效，导致无反应性或耐药。双特异性抗体（BsAbs）代表了一种创新的免疫治疗策略，具有改善癌症治疗结果的巨大潜力。与单克隆抗体不同，bsab可以同时抑制多种促肿瘤途径，靶向免疫检查点以减轻耐药性，并与两种不同的抗原结合，从而增强特异性，同时最大限度地减少脱靶效应。此外，与两种单独的单克隆抗体联合使用相比，bsab更具成本效益，毒性更小。近几十年来，bsab在临床开发方面取得了显著进展。一些bsab，如blinatumumab， mosunetuzumab, teclistamab, glofitamab, epcoritamab, talquetamab, ivonescimab, cadonilimab, tarlatamab， zenocutuzumab和catumaxomab，已经在临床试验中取得了显著的成功。本文综述了临床批准的bsab，并对其在癌症治疗中的应用进行了全面总结。

{"title":"Advances in the clinical application of bispecific antibodies in cancer therapy","authors":"Shiqi Zhou , Feng Li , Mengke Niu , Kongming Wu , Tianye Li , Ming Yi","doi":"10.1016/j.isci.2025.114203","DOIUrl":"10.1016/j.isci.2025.114203","url":null,"abstract":"<div><div>Cancer immunotherapy has emerged as one of the most groundbreaking advancements. However, the tumor microenvironment (TME) is often dominated by various immunosuppressive factors, compromising the efficacy of single-target therapies, leading to non-responsiveness or resistance. Bispecific antibodies (BsAbs) represent an innovative immunotherapeutic strategy with enormous potential for improving cancer treatment outcomes. Unlike monoclonal antibodies, BsAbs can concurrently inhibit multiple pro-tumor pathways, target immune checkpoints to mitigate resistance, and bind to two distinct antigens, thereby enhancing specificity while minimizing off-target effects. Moreover, BsAbs are more cost-efficient and less toxic compared to the use of two separate monoclonal antibodies in combination. In recent decades, BsAbs have made remarkable progress in clinical development. Several BsAbs, such as blinatumomab, mosunetuzumab, teclistamab, glofitamab, epcoritamab, talquetamab, ivonescimab, cadonilimab, tarlatamab, zenocutuzumab, and catumaxomab, have achieved notable success in clinical trials. This review highlights clinically approved BsAbs and provides a comprehensive summary of their therapeutic applications in cancer treatment.</div></div>","PeriodicalId":342,"journal":{"name":"iScience","volume":"28 12","pages":"Article 114203"},"PeriodicalIF":4.1,"publicationDate":"2025-11-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145733378","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Woody oil crop domestication in the genus Camellia: Perspectives on future breeding 油茶属木本油料作物的驯化：未来育种展望

IF 4.1 2区综合性期刊 Q1 MULTIDISCIPLINARY SCIENCES

iScience

Pub Date : 2025-11-25 DOI: 10.1016/j.isci.2025.114205

Yao Zhao , Kaifeng Xing , Jun Zhou , Xia Dong , Shengyuan Qin , Haoxing Xie , Li Cheng , Huan Yang , Jie Xiao , Jian Zhang , Jun Rong

Oilseed Camellia, including diverse species within the Camellia genus, valued for high seed-oil content, is the dominant woody oil crop in China. We reviewed the key issues in oilseed Camellia domestication and its origin. Major oilseed Camellia species are polyploids within Camellia sect. Paracamellia, with the highest species diversity in the Nanling Mountains areas. Oilseed Camellia began to be cultivated as oil crops 400 years ago in the subtropical hilly areas of the Yangtze River Basin. Domestication may have led to significantly larger fruits and higher oleic acid content, but the genetic mechanisms remain unknown. Interspecific hybridization and polyploidization may have contributed to the origin of major oilseed Camellia, generating rich diversity in fruit traits. Perspectives are proposed on future breeding, including resolving molecular mechanisms of key traits, improving abiotic tolerance, and creating new germplasms via hybridizations. This review may guide the efficient exploration and utilization of genetic resources for improving oilseed Camellia.

油茶属植物种类繁多，种子含油量高，是中国主要的木本油料作物。本文综述了油茶驯化及其起源研究中的关键问题。油籽油茶属植物以多倍体为主，属副油茶科，物种多样性以南岭山区最高。400年前，在长江流域的亚热带丘陵地区，油茶作为油料作物开始被种植。驯化可能导致果实较大，油酸含量较高，但遗传机制尚不清楚。种间杂交和多倍体化可能是主要油籽油茶的起源，产生了丰富的果实性状多样性。对今后的育种工作提出了展望，包括解决关键性状的分子机制，提高非生物耐受性，通过杂交创造新的种质。本文综述可为油茶种质资源的有效开发和利用提供指导。

{"title":"Woody oil crop domestication in the genus Camellia: Perspectives on future breeding","authors":"Yao Zhao , Kaifeng Xing , Jun Zhou , Xia Dong , Shengyuan Qin , Haoxing Xie , Li Cheng , Huan Yang , Jie Xiao , Jian Zhang , Jun Rong","doi":"10.1016/j.isci.2025.114205","DOIUrl":"10.1016/j.isci.2025.114205","url":null,"abstract":"<div><div>Oilseed <em>Camellia</em>, including diverse species within the <em>Camellia</em> genus, valued for high seed-oil content, is the dominant woody oil crop in China. We reviewed the key issues in oilseed <em>Camellia</em> domestication and its origin. Major oilseed <em>Camellia</em> species are polyploids within <em>Camellia</em> sect. <em>Paracamellia</em>, with the highest species diversity in the Nanling Mountains areas. Oilseed <em>Camellia</em> began to be cultivated as oil crops 400 years ago in the subtropical hilly areas of the Yangtze River Basin. Domestication may have led to significantly larger fruits and higher oleic acid content, but the genetic mechanisms remain unknown. Interspecific hybridization and polyploidization may have contributed to the origin of major oilseed <em>Camellia</em>, generating rich diversity in fruit traits. Perspectives are proposed on future breeding, including resolving molecular mechanisms of key traits, improving abiotic tolerance, and creating new germplasms via hybridizations. This review may guide the efficient exploration and utilization of genetic resources for improving oilseed <em>Camellia</em>.</div></div>","PeriodicalId":342,"journal":{"name":"iScience","volume":"28 12","pages":"Article 114205"},"PeriodicalIF":4.1,"publicationDate":"2025-11-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145733274","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

From skin lesions to multi-organ involvement: Organ tropism and pathogenesis of mpox virus 从皮肤病变到多器官受累：痘病毒的器官趋向性和发病机制

IF 4.1 2区综合性期刊 Q1 MULTIDISCIPLINARY SCIENCES

iScience

Pub Date : 2025-11-25 DOI: 10.1016/j.isci.2025.114209

Anna-Lena Rupp , Jo Paul Meister , Julian Schulze Zur Wiesch , Toni Luise Meister

Mpox virus (MPXV), the causative agent of mpox, is a re-emerging zoonotic orthopoxvirus. Until 2022, infections were largely confined to endemic regions in Central and West Africa. However, following phylogenetic divergence and a subsequent global outbreak in 2022 caused by clade IIb, distinct from the clade I-driven resurgence in the Democratic Republic of the Congo in 2024, MPXV has emerged as a significant global health concern. While mpox is primarily characterized by its distinctive skin lesions, growing clinical and experimental evidence suggests that MPXV can replicate and disseminate to further organ systems, potentially contributing to disease severity. Despite this growing knowledge, the mechanisms underlying MPXV organ tropism and pathogenesis remain poorly understood, posing ongoing challenges for research and clinical management. This review summarizes the latest clinical, pathological, and experimental findings to provide an updated understanding of MPXV tropism and pathogenesis.

m痘病毒（MPXV）是一种重新出现的人畜共患正痘病毒，是引起m痘的病原体。直到2022年，感染主要局限于中非和西非的流行地区。然而，在发生系统发育差异并随后在2022年由IIb进化支（不同于2024年在刚果民主共和国由i进化支引起的死灰复燃）引起的全球疫情之后，MPXV已成为一个重大的全球卫生问题。虽然m痘的主要特征是其独特的皮肤病变，但越来越多的临床和实验证据表明，MPXV可以复制并传播到其他器官系统，可能导致疾病的严重程度。尽管人们对MPXV的器官倾向和发病机制了解越来越多，但对MPXV的器官倾向和发病机制仍然知之甚少，这给研究和临床管理带来了持续的挑战。本文综述了最新的临床、病理和实验结果，以提供对MPXV嗜性和发病机制的最新认识。

引用次数: 0

首页上一页

下一页尾页

类型

全部化学•材料生命科学医学物理工程技术环境•农林材料科学地球科学法学管理学化学环境科学与生态学计算机科学教育学经济学农林科学人文科学生物学数学物理与天体物理心理学综合性期刊其他工业工程理学历史学农学文学信息工程

数据库

全部 ACS Publications Elsevier ieeexplore Springer The Royal Society of Chemistry Wiley

期刊

iScience

全部 Acc. Chem. Res. ACS Applied Bio Materials ACS Appl. Electron. Mater. ACS Appl. Energy Mater. ACS Appl. Mater. Interfaces ACS Appl. Nano Mater. ACS Appl. Polym. Mater. ACS BIOMATER-SCI ENG ACS Catal. ACS Cent. Sci. ACS Chem. Biol. ACS Chemical Health & Safety ACS Chem. Neurosci. ACS Comb. Sci. ACS Earth Space Chem. ACS Energy Lett. ACS Infect. Dis. ACS Macro Lett. ACS Mater. Lett. ACS Med. Chem. Lett. ACS Nano ACS Omega ACS Photonics ACS Sens. ACS Sustainable Chem. Eng. ACS Synth. Biol. Anal. Chem. BIOCHEMISTRY-US Bioconjugate Chem. BIOMACROMOLECULES Chem. Res. Toxicol. Chem. Rev. Chem. Mater. CRYST GROWTH DES ENERG FUEL Environ. Sci. Technol. Environ. Sci. Technol. Lett. Eur. J. Inorg. Chem. IND ENG CHEM RES Inorg. Chem. J. Agric. Food. Chem. J. Chem. Eng. Data J. Chem. Educ. J. Chem. Inf. Model. J. Chem. Theory Comput. J. Med. Chem. J. Nat. Prod. J PROTEOME RES J. Am. Chem. Soc. LANGMUIR MACROMOLECULES Mol. Pharmaceutics Nano Lett. Org. Lett. ORG PROCESS RES DEV ORGANOMETALLICS J. Org. Chem. J. Phys. Chem. J. Phys. Chem. A J. Phys. Chem. B J. Phys. Chem. C J. Phys. Chem. Lett. Analyst Anal. Methods Biomater. Sci. Catal. Sci. Technol. Chem. Commun. Chem. Soc. Rev. CHEM EDUC RES PRACT CRYSTENGCOMM Dalton Trans. Energy Environ. Sci. ENVIRON SCI-NANO ENVIRON SCI-PROC IMP ENVIRON SCI-WAT RES Faraday Discuss. Food Funct. Green Chem. Inorg. Chem. Front. Integr. Biol. J. Anal. At. Spectrom. J. Mater. Chem. A J. Mater. Chem. B J. Mater. Chem. C Lab Chip Mater. Chem. Front. Mater. Horiz. MEDCHEMCOMM Metallomics Mol. Biosyst. Mol. Syst. Des. Eng. Nanoscale Nanoscale Horiz. Nat. Prod. Rep. New J. Chem. Org. Biomol. Chem. Org. Chem. Front. PHOTOCH PHOTOBIO SCI PCCP Polym. Chem.

﹀