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Designing a novel method based on multiplex PCR for detecting various meat of birds in processed ground meat products 一种基于多重聚合酶链式反应检测加工肉制品中各种禽类肉类的新方法的设计
IF 3.3 Q2 FOOD SCIENCE & TECHNOLOGY Pub Date : 2023-07-13 DOI: 10.1016/j.fochms.2023.100177
Negin Rajaei , Abbas Doosti

Falsified food directly influences wildlife, fair trade, religion, and the health of society. Here, we report a multiplex polymerase chain reaction to evaluate the accurate determination of seven species of bird meat in meals on a single assay platform. To amplify segments of DNA from Columba livia, Corvus moneduloides, Gallus gallus, Coturnix japonica, Phasianus colchicus, Struthio camelus, and Meleagris gallopavo meats, respectively, a total of seven sets of species-specific primers targeting the mitochondrial and cytochrome b genes were developed. Gel photographs and electrochromatography from an Experion Bioanalyzer were used to identify all PCR products. Species specificity checks discovered no cross-species amplification. The applicability of its screening to find target species in processed food was shown in commercial and model meatballs. A validation study revealed that the test is reliable, quick, affordable, repeatable, specific, and accurate down to 50,000 mitochondrial copies. It might be used for raw meats and products involving processed and severely deteriorated food samples. The customers, the food business, and law enforcement would all benefit immensely from this suggested approach.

伪造食品直接影响野生动物、公平贸易、宗教和社会健康。在这里,我们报道了一种多重聚合酶链式反应,以评估在单一检测平台上对膳食中七种鸟肉的准确测定。为了扩增分别来自鹅肝、蒙氏Corvus moneduloides、Gallus Gallus、Coturnix japonica、Phasianus colchicus、Struthio camelus和Meleagris gallopavo肉的DNA片段,共开发了7套针对线粒体和细胞色素b基因的物种特异性引物。使用Experion生物分析仪的凝胶照片和电色谱法鉴定所有PCR产物。物种特异性检查未发现跨物种扩增。其筛选在加工食品中寻找目标物种的适用性在商业和模型肉丸中得到了证明。一项验证研究表明,该测试可靠、快速、经济实惠、可重复、特异性强,准确度低至50000个线粒体拷贝。它可能用于生肉和涉及加工和严重变质食品样品的产品。顾客、食品企业和执法部门都将从这一建议中受益匪浅。
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引用次数: 0
Comprehensive analysis of transcriptome and metabolome provides insights into the stress response mechanisms of apple fruit to postharvest impact damage 转录组和代谢组的综合分析为深入了解苹果果实采后冲击损伤的应激反应机制提供了依据
IF 3.3 Q2 FOOD SCIENCE & TECHNOLOGY Pub Date : 2023-07-03 DOI: 10.1016/j.fochms.2023.100176
Zhichao Yang , Menghua Lin , Xiangzheng Yang , Di Wu , Kunsong Chen

An integrated analysis of the transcriptome and metabolome was conducted to investigate the underlying mechanisms of apple fruit response to impact damage stress. During the post-damage storage, a total of 124 differentially expressed genes (DEGs) were identified, which were mainly annotated in 13 pathways, including phenylpropanoid biosynthesis. Besides, 175 differentially expressed metabolites (DEMs), including 142 up-regulated and 33 down-regulated metabolites, exhibited significant alteration after impact damage. The DEGs and DEMs were simultaneously annotated in 7 metabolic pathways, including flavonoid biosynthesis. Key genes in the volatile esters and flavonoid biosynthesis pathways were revealed, which may play a crucial role in the coping mechanisms of apple fruit under impact damage stress. Moreover, 13 ABC transporters were significantly upregulated, indicating that ABC transporters may contribute to the transportation of secondary metabolites associated with response to impact damage stress. The results may elucidate the comprehension of metabolic networks and molecular mechanisms in apple fruits that have undergone impact damage.

对转录组和代谢组进行了综合分析,以研究苹果果实对冲击损伤应激反应的潜在机制。在损伤后储存过程中,共鉴定出124个差异表达基因(DEG),这些基因主要注释在13个途径中,包括苯丙烷生物合成。此外,175种差异表达代谢物(DEM),包括142种上调和33种下调的代谢物,在撞击损伤后表现出显著的变化。DEGs和DEMs在包括类黄酮生物合成在内的7种代谢途径中同时被注释。揭示了挥发性酯类和类黄酮生物合成途径中的关键基因,这些基因可能在苹果果实在冲击损伤胁迫下的应对机制中起着至关重要的作用。此外,13种ABC转运蛋白显著上调,表明ABC转运蛋白可能有助于运输与冲击损伤应激反应相关的次级代谢产物。这些结果可能阐明对遭受冲击损伤的苹果果实的代谢网络和分子机制的理解。
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引用次数: 1
Chemical constituents of green teas processed from albino tea cultivars with white and yellow shoots 从白色和黄色芽的白化茶品种加工的绿茶的化学成分
IF 3.3 Q2 FOOD SCIENCE & TECHNOLOGY Pub Date : 2022-12-30 DOI: 10.1016/j.fochms.2022.100143
Jihong Yang , Hanchen Zhou , Yaqin Liu, Hui Wang, Yujie Xu, Jianqin Huang, Pandeng Lei

Green tea processed from albino tea varieties often has umami taste and fresh aroma. This study identified green teas made from two types of albino tea cultivar, one having the white shoots (called Naibai, NB) and the other having the yellow shoots (called Huangjinya, HJY). Taste compounds analyses showed that galloylated catechins were highly concentrated in HJY green teas, whereas non-galloylated catechins and amino acids were more abundant in NB green teas. CsTA (involved in the catabolism of galloylated catechins) showed high expression in HJY tea shoots, resulting in gallic acid as a precursor for β-glucogallin biosynthesis being abundant in HJY. CsPDX2.1 (responsible for theanine hydrolyzation) had a lower expression level in NB than HJY shoots. Fatty acid–derived volatiles (FADVs), glycosidically bound volatiles (GBVs) and carotenoid–derived volatiles (CDVs) were highly concentrated in HJY green teas, whereas amino acids–derived volatiles were highly concentrated in NB green teas.

用白化茶品种加工而成的绿茶往往具有鲜味和清新的香气。本研究鉴定了由两种白化茶品种制成的绿茶,一种有白色的芽(称为Naibai, NB),另一种有黄色的芽(称为Huangjinya, HJY)。味觉成分分析表明,沪江绿茶中没食子酸儿茶素含量较高,而NB绿茶中非没食子酸儿茶素和氨基酸含量较高。CsTA(参与没食子酸儿茶素的分解代谢)在HJY茶叶中高表达,导致没食子酸作为β-胰高糖素生物合成的前体在HJY中丰富。CsPDX2.1(负责茶氨酸水解)在NB中的表达量低于HJY。脂肪酸衍生挥发物(FADVs)、糖苷结合挥发物(GBVs)和类胡萝卜素衍生挥发物(cdv)在HJY绿茶中含量较高,而氨基酸衍生挥发物在NB绿茶中含量较高。
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引用次数: 1
Comparison of oxidative stress-mitochondria-mediated tenderization in two different bovine muscles during aging 氧化应激-线粒体介导的两种不同牛肌肉衰老过程中嫩化的比较
IF 3.3 Q2 FOOD SCIENCE & TECHNOLOGY Pub Date : 2022-12-30 DOI: 10.1016/j.fochms.2022.100131
Zhenjiang Ding , Qichao Wei , Chunmei Liu , Chunhui Zhang , Feng Huang

The aim of this study was to investigate the differences in the effects of mitochondria-involved energy metabolism and caspases activation on postmortem tenderness in different muscle fiber types. Beef Longissimus thoracis (LT) and Psoas major (PM) muscles showed significant difference in mitochondrial function. Our data revealed that PM suffered from higher levels of reactive oxygen species (ROS) earlier than LT, causing faster mitochondrial swelling and rupture. Additionally, faster metabolism of ATP-related compounds and activation of caspase-9 appeared in PM, but the activity of caspase-3 in PM was lower than that in LT. Differences in myofibril fragmentation index (MFI) of LT and PM at different aging stages suggested that energy metabolism and caspases activities may play a role in tenderness at different aging stages. These results indicated that oxidative stress-mitochondria-mediated tenderization process could be muscle-specific.

本研究的目的是探讨线粒体参与的能量代谢和半胱天冬酶激活对不同肌肉纤维类型死后压痛的影响差异。牛肉胸最长肌(LT)和大腰肌(PM)线粒体功能差异显著。我们的数据显示,PM比LT更早遭受更高水平的活性氧(ROS),导致线粒体肿胀和破裂更快。此外,PM中atp相关化合物的代谢和caspase-9的激活均较快,而PM中caspase-3的活性低于LT。不同衰老阶段LT和PM肌原纤维断裂指数(MFI)的差异提示不同衰老阶段能量代谢和caspase活性可能在嫩痛中起作用。这些结果表明,氧化应激-线粒体介导的嫩化过程可能是肌肉特异性的。
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引用次数: 1
Identification of the genes associated with β-glucan synthesis and accumulation during grain development in barley 大麦籽粒发育过程中β-葡聚糖合成与积累相关基因的鉴定
IF 3.3 Q2 FOOD SCIENCE & TECHNOLOGY Pub Date : 2022-12-30 DOI: 10.1016/j.fochms.2022.100136
La Geng , Xinyi He , Lingzhen Ye , Guoping Zhang

The presence of β-glucan in barley grains is one of its important quality traits. Lower β-glucan content is required for the barley used in beer and feed production, while higher β-glucan content is beneficial for food barley. Although intensive research has been carried out on the genotypic and environmental differences in β-glucan content in barley grains, little information is available on the molecular mechanisms underlying their genotypic differences and genetic regulation of β-glucan synthesis and accumulation. In this study, RNA sequencing analysis was conducted to compare the transcriptome profiles of two barley genotypes (BCS192 and BCS297) that greatly differ in grain β-glucan content, in order to identify the key genes responsible for β-glucan synthesis and accumulation during grain development. The results showed that carbohydrate metabolic processes and starch and sucrose metabolism play significant roles in β-glucan synthesis. The identified differently expressed genes (DEGs), which are closely associated with grain β-glucan content, are mainly involved in hydrolase activity and glucan metabolic processes. In addition, β-glucan accumulation in barley grains is predominantly regulated by photosynthesis and carbon metabolism. The DEGs identified in this study and their functions may provide new insights into the molecular mechanisms of β-glucan synthesis and genotypic differences in barley grains.

大麦籽粒中β-葡聚糖的存在是其重要的品质性状之一。用于啤酒和饲料生产的大麦需要较低的β-葡聚糖含量,而较高的β-葡聚糖含量有利于食用大麦。虽然对大麦籽粒中β-葡聚糖含量的基因型和环境差异进行了深入的研究,但对其基因型差异的分子机制以及β-葡聚糖合成和积累的遗传调控却知之甚少。本研究通过RNA测序分析,比较籽粒β-葡聚糖含量差异较大的两个大麦基因型(BCS192和BCS297)的转录组谱,以确定籽粒发育过程中负责β-葡聚糖合成和积累的关键基因。结果表明,碳水化合物代谢过程、淀粉和蔗糖代谢过程在β-葡聚糖合成中起重要作用。所鉴定的不同表达基因(DEGs)与籽粒β-葡聚糖含量密切相关,主要参与水解酶活性和葡聚糖代谢过程。大麦籽粒β-葡聚糖积累主要受光合作用和碳代谢的调控。本研究鉴定的deg及其功能可能为大麦籽粒β-葡聚糖合成的分子机制和基因型差异提供新的见解。
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引用次数: 2
Role of integrated omics in unravelling fruit stress and defence responses during postharvest: A review 整合组学在揭示果实采后胁迫和防御反应中的作用综述
IF 3.3 Q2 FOOD SCIENCE & TECHNOLOGY Pub Date : 2022-12-30 DOI: 10.1016/j.fochms.2022.100118
Zinash A Belay , Oluwafemi James Caleb

Fruit are susceptible to quality loss and deterioration after harvest due to high metabolic and physiological activities. Over the last four decades various postharvest treatments have ensured maintenance of quality, control of diseases or decay by slowing down the postharvest ripening and senesce. The fruit quality change during postharvest however, has been mostly explored using physicochemical characteristics. Considering the complexity of fruit physiology and metabolism, the application of omics techniques could aid the in-depth analysis and understanding of fruit quality change during postharvest treatment. Therefore, this review presents recent information on the application of integrated omics (transcriptomics, proteomics, and metabolomics) in postharvest research, with an overview on fruit quality and safety. Trends in omics data analysis for fruit during postharvest handling was highlighted. The role of integrated omics in improving our understanding of fruit response during natural postharvest progression (towards decay) during storage, as well as in case of induced responses due to the application of biocontrols was discussed. The article concluded with the outlooks of future studies on the application of integrated omics as the catalyst for innovative postharvest solutions.

果实在收获后由于代谢和生理活动旺盛,易发生品质损失和变质。在过去的四十年中,各种采后处理通过减缓采后成熟和衰老来确保保持质量,控制疾病或腐烂。而果实采后品质变化主要是利用理化特性来研究的。考虑到果实生理代谢的复杂性,应用组学技术有助于深入分析和理解采后处理过程中果实品质的变化。因此,本文综述了整合组学(转录组学、蛋白质组学和代谢组学)在采后研究中的应用,并对果实质量和安全进行了综述。强调了水果采后处理过程中组学数据分析的趋势。讨论了整合组学在提高我们对果实在采后自然进程(走向腐烂)储存期间的反应以及由于应用生物防治而引起的诱导反应的理解中的作用。文章最后展望了整合组学作为创新采后解决方案催化剂的未来研究。
{"title":"Role of integrated omics in unravelling fruit stress and defence responses during postharvest: A review","authors":"Zinash A Belay ,&nbsp;Oluwafemi James Caleb","doi":"10.1016/j.fochms.2022.100118","DOIUrl":"10.1016/j.fochms.2022.100118","url":null,"abstract":"<div><p>Fruit are susceptible to quality loss and deterioration after harvest due to high metabolic and physiological activities. Over the last four decades various postharvest treatments have ensured maintenance of quality, control of diseases or decay by slowing down the postharvest ripening and senesce. The fruit quality change during postharvest however, has been mostly explored using physicochemical characteristics. Considering the complexity of fruit physiology and metabolism, the application of omics techniques could aid the in-depth analysis and understanding of fruit quality change during postharvest treatment. Therefore, this review presents recent information on the application of integrated omics (transcriptomics, proteomics, and metabolomics) in postharvest research, with an overview on fruit quality and safety. Trends in omics data analysis for fruit during postharvest handling was highlighted. The role of integrated omics in improving our understanding of fruit response during natural postharvest progression (towards decay) during storage, as well as in case of induced responses due to the application of biocontrols was discussed. The article concluded with the outlooks of future studies on the application of integrated omics as the catalyst for innovative postharvest solutions.</p></div>","PeriodicalId":34477,"journal":{"name":"Food Chemistry Molecular Sciences","volume":"5 ","pages":"Article 100118"},"PeriodicalIF":3.3,"publicationDate":"2022-12-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/6f/bd/main.PMC9278069.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40530327","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 8
β-carotene genetically-enriched lyophilized orange juice increases antioxidant capacity and reduces β-amyloid proteotoxicity and fat accumulation in Caenorhabditis elegans 富含β-胡萝卜素基因的冻干橙汁增加了秀丽隐杆线虫的抗氧化能力,减少了β-淀粉样蛋白毒性和脂肪积累
IF 3.3 Q2 FOOD SCIENCE & TECHNOLOGY Pub Date : 2022-12-30 DOI: 10.1016/j.fochms.2022.100141
Iolanda Raquel Ferreira Paulo , Ricardo Basílio de Oliveira Caland , Cesar Orlando Muñoz Cadavid , Giovanna Martins Melo , Liliane Soares De Castro Bezerra , Elsa Pons , Leandro Peña , Riva de Paula Oliveira

Citrus sinensis orange juice is an excellent dietary source of β-carotene, a well-known antioxidant. However, β-carotene concentrations are relatively low in most cultivars. We developed a new orange through metabolic engineering strategy (GS) with 33.72-fold increase in β-carotene content compared to its conventional counterpart (CV). Using Caenorhabditis elegans, we found that animals treated with GS showed a greater reduction in intracellular reactive oxygen species (ROS) which is associated with a greater resistance to oxidative stress and induction of the expression of antioxidant genes. Moreover, animals treated with GS orange showed a more effective protection against β-amyloid proteotoxicity and greater hypolipidemic effect under high glucose diet compared to animals treated with CV. These data demonstrate that the increased amount of β-carotene in orange actually provides a greater beneficial effect in C. elegans and a valuable proof of principle to support further studies in mammals and humans.

柑桔汁是β-胡萝卜素的极佳膳食来源,β-胡萝卜素是一种众所周知的抗氧化剂。然而,大多数品种的β-胡萝卜素浓度相对较低。我们通过代谢工程策略(GS)培育出了一种β-胡萝卜素含量比常规橙子(CV)增加33.72倍的新橙子。通过对秀丽隐杆线虫的研究,我们发现经GS处理的动物细胞内活性氧(ROS)的减少幅度更大,这与更强的氧化应激抗性和诱导抗氧化基因的表达有关。此外,与CV处理的动物相比,GS橙处理的动物在高糖饮食下表现出更有效的β-淀粉样蛋白毒性和更大的降血脂作用。这些数据表明,橙子中β-胡萝卜素含量的增加实际上对秀丽隐杆线虫有更大的有益作用,并为进一步在哺乳动物和人类中进行研究提供了有价值的原理证明。
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引用次数: 1
Bioaccessibility assay, antioxidant activity and consumer-oriented sensory analysis of Beta vulgaris by-product encapsulated in Ca(II)-alginate beads for different foods 海藻酸Ca(II)-海藻酸微球包封的β - vulgaris副产物在不同食品中的生物可及性、抗氧化活性和消费者感官分析
IF 3.3 Q2 FOOD SCIENCE & TECHNOLOGY Pub Date : 2022-12-30 DOI: 10.1016/j.fochms.2022.100140
Tatiana Rocio Aguirre-Calvo , Natalia Sosa , Tamara Anahí López , María Ximena Quintanilla-Carvajal , Mercedes Perullini , Patricio Román Santagapita

Bioaccessibility analysis and antioxidant activity along in vitro digestion and a consumer-oriented sensory analysis were conducted in three potential functional foods based on Ca(II)-alginate beads containing bioactive compounds extracted from beet stems. Ca(II)-alginate beads per se, and two selected products (cookies and turkish delights supplemented with the beads) were prepared. Regarding the beads, among the attributes rated by consumers, visual appreciation predominates, being color in the just-as-right (JAR) category and in the like preference. Instead, both flavor and sweet taste were attributes highly penalized and should be improved in beads to be accepted as food per se. A higher percentage of customers preferred cookies and turkish delights instead of only beads, considering global satisfaction. Regarding in vitro digestion, there was a significant content of phenolic compounds in the products with beads, showing a bioaccessibility greater than 80% (for cookies) and 26% (for turkish delights). Also, the antioxidant capacity measured by ABTS ranged between 50 and 109% for cookies and turkish delights, being lower when measured by FRAP (between 20 and 30%, respectively). Thus, including the beads with beet stem extract in both products leads to a significant increase in the content of phenolic compounds and in the antioxidant capacity compared to their counterparts, protecting the compound during oral and gastric phases. These results allow the generation of improved Ca(II)-alginate systems with promising functional properties for the development of ingredients and functional foods.

以甜菜茎中提取的含有生物活性化合物的海藻酸钙微球为原料,对三种潜在的功能食品进行了生物可及性分析、体外消化抗氧化活性分析和消费者感官分析。Ca(II)-海藻酸盐珠本身,和两种选定的产品(饼干和土耳其美食补充珠)制备。对于珠子,在消费者评价的属性中,视觉欣赏占主导地位,在just-as-right (JAR)类别和类似偏好中,颜色占主导地位。相反,味道和甜味都是受到高度惩罚的属性,应该在珠子上加以改进,以被接受为食物本身。考虑到全球满意度,更高比例的顾客更喜欢饼干和土耳其美食,而不仅仅是珠子。在体外消化方面,含珠产品中酚类化合物含量显著,生物可及性大于80%(饼干)和26%(土耳其甜点)。此外,ABTS测定的饼干和土耳其甜点的抗氧化能力在50%到109%之间,而FRAP测定的抗氧化能力较低(分别在20%到30%之间)。因此,在两种产品中加入甜菜茎提取物的珠子,与它们的同类相比,酚类化合物的含量和抗氧化能力显著增加,在口服和胃期保护化合物。这些结果允许生成改进的Ca(II)-海藻酸盐体系,具有开发成分和功能食品的有前途的功能特性。
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引用次数: 0
Transcriptome sequencing and gas chromatography–mass spectrometry analyses provide insights into β-caryophyllene biosynthesis in Brassica campestris 转录组测序和气相色谱-质谱分析提供了油菜β-石竹烯生物合成的见解
IF 3.3 Q2 FOOD SCIENCE & TECHNOLOGY Pub Date : 2022-12-30 DOI: 10.1016/j.fochms.2022.100129
Haibin Wang , Chen Zong , Aimei Bai , Shuilin Yuan , Yan Li , Zhanghong Yu , Ruiping Tian , Tongkun Liu , Xilin Hou , Ying Li

Sesquiterpenes are important defensive secondary metabolites and aroma components. However, limited information is available on the mechanism of sesquiterpene formation and composition in the non-heading Chinese cabbage (NHCC) leaf. Therefore, headspace solid-phase microextraction/gas chromatography–mass spectrometry (HS-SPME/GC–MS) combined with transcriptome analysis was used to study the mechanism of volatile organic compound formation. A total of 26 volatile organic compounds were identified in two NHCC cultivars ‘SZQ’ and ‘XQC’ and their F1 hybrids. Among these, sesquiterpene β-caryophyllene was identified only in ‘XQC’ and F1. Five genes encoding caryophyllene synthase were identified. The candidate β-caryophyllene synthase genes BcTPSa11 and BcTPSa21 had high expression levels only in ‘XQC’ and F1. In addition, several transcription factors of MYB-related, MYB, bHLH, and AP2/ERF families were identified by co-expression, suggesting that they regulate β-caryophyllene biosynthesis. Our results provide a molecular basis for sesquiterpene biosynthesis as well as insights into the regulatory network of β-caryophyllene in NHCC.

倍半萜是重要的防御性次生代谢产物和香气成分。然而,关于倍半萜在不结籽大白菜(NHCC)叶片中形成和组成的机理研究有限。因此,采用顶空固相微萃取/气相色谱-质谱法(HS-SPME/ GC-MS)结合转录组分析对挥发性有机物的形成机制进行研究。从2个NHCC品种SZQ和XQC及其F1杂种中共鉴定出26种挥发性有机物。其中倍半萜β-石竹烯仅在‘XQC’和F1中得到鉴定。鉴定出5个编码石竹烯合成酶的基因。候选β-石竹烯合成酶基因BcTPSa11和BcTPSa21仅在‘XQC’和F1中高表达。此外,通过共表达鉴定了MYB相关、MYB、bHLH和AP2/ERF家族的几个转录因子,表明它们调节β-石竹烯的生物合成。我们的研究结果为倍半萜的生物合成提供了分子基础,并揭示了NHCC中β-石竹烯的调控网络。
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引用次数: 2
Effects of galactosyltransferase on EPS biosynthesis and freeze-drying resistance of Lactobacillus acidophilus NCFM 半乳糖转移酶对嗜酸乳杆菌NCFM EPS生物合成及冻干抗性的影响
IF 3.3 Q2 FOOD SCIENCE & TECHNOLOGY Pub Date : 2022-12-30 DOI: 10.1016/j.fochms.2022.100145
Lingyu Kong , Yuze Huang , Xiaoqun Zeng , Congyan Ye , Zhen Wu , Yuxing Guo , Daodong Pan

Galactosyltransferase (GalT) is an important enzyme in synthesizing exopolysaccharide (EPS), the major polymer of biofilms protecting cells from severe conditions. However, the contribution to, and regulatory mechanism of GalT, in stressor resistance are still unclear. Herein, we successfully overexpressed GalT in Lactobacillus acidophilus NCFM by genetic engineering. The GalT activity and freeze-drying survival rate of the recombinant strain were significantly enhanced. The EPS yield also increased by 17.8%, indicating a positive relationship between freeze-drying resistance and EPS. RNA-Seq revealed that GalT could regulate the flux of the membrane transport system, pivotal sugar-related metabolic pathways, and promote quorum sensing to facilitate EPS biosynthesis, which enhanced freeze-drying resistance. The findings concretely prove that the mechanism of GalT regulating EPS biosynthesis plays an important role in protecting lactic acid bacteria from freeze-drying stress.

半乳糖转移酶(GalT)是合成胞外多糖(EPS)的重要酶,EPS是保护细胞免受恶劣环境影响的生物膜的主要聚合物。然而,高泌泌素在应激抵抗中的作用及其调控机制尚不清楚。本研究通过基因工程成功地在嗜酸乳杆菌NCFM中过表达了GalT。重组菌株的GalT活性和冻干存活率显著提高。EPS产量也提高了17.8%,表明抗冻干性与EPS呈正相关。RNA-Seq结果显示,GalT可以调节膜运输系统的通量,关键的糖相关代谢途径,促进群体感应,促进EPS的生物合成,从而增强其抗冻干能力。研究结果具体证明了GalT调控EPS生物合成的机制在保护乳酸菌免受冻干胁迫中起着重要作用。
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引用次数: 5
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Food Chemistry Molecular Sciences
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