Food Chemistry Molecular Sciences最新文献_第2页

Phenolic extract from olive mill wastewater sustains mitochondrial bioenergetics upon oxidative insult 橄榄厂废水酚提取物维持线粒体生物能量氧化损伤。

IF 4.1 Q2 FOOD SCIENCE & TECHNOLOGY

Food Chemistry Molecular Sciences

Pub Date : 2024-12-11 DOI: 10.1016/j.fochms.2024.100234

Iolanda Rita Infantino , Salvatore Antonio Maria Cubisino , Stefano Conti Nibali , Paola Foti , Marianna Flora Tomasello , Silvia Boninelli , Giuseppe Battiato , Andrea Magrì , Angela Messina , Flora Valeria Romeo , Cinzia Caggia , Vito De Pinto , Simona Reina

In the last few years, many efforts have been devoted to the recovery and valorization of olive oil by-products because of their potentially high biological value. The olive mill wastewater (OMWW), a dark-green brown colored liquid that mainly consists of olive fruit vegetation water, is particularly exploited in this regard for its great content in phenolic compounds with strong antioxidant properties. In our previous work, we produced different OMWW fractions enriched in hydroxytyrosol- and hydroxytyrosol/oleuropein (i.e. C and OPE extracts, respectively) that exhibited considerable anti-microbial and radical-scavenging activities in vitro. Based on these findings, the present study aimed to assess the impact of C and OPE samples on mitochondrial function and oxidative stress response in mouse fibroblast-like cells (NCTC). Accordingly, OMWW phenolic extracts proved to enhance mitochondrial biogenesis and to reduce cellular sensitivity to hydrogen peroxide. Moreover, high-resolution respirometry experiments first time revealed the efficiency of OMWW phenols recovered by selective resin extraction in preventing mitochondrial respiration failure upon oxidative insult. Collected data definitely demonstrate the bioactivity of our phenolic-rich fractions, supporting the advantages of reusing the olive mill wastewater to generate, at low-cost, high added value molecules that could be useful for the improvement of health and nutrition products.

在过去的几年里，由于橄榄油副产品具有潜在的高生物价值，许多人致力于橄榄油副产品的回收和增值。橄榄厂废水（OMWW）是一种深绿色的液体，主要由橄榄果实植被水组成，由于其含有大量具有强抗氧化性能的酚类化合物，因此在这方面得到了特别的利用。在我们之前的工作中，我们生产了不同的富含羟基酪醇-和羟基酪醇/橄榄苦苷的OMWW组分（分别为C和OPE提取物），它们在体外表现出相当大的抗微生物和自由基清除活性。基于这些发现，本研究旨在评估C和OPE样品对小鼠成纤维细胞样细胞（NCTC）线粒体功能和氧化应激反应的影响。因此，OMWW酚提取物被证明可以促进线粒体的生物发生，降低细胞对过氧化氢的敏感性。此外，高分辨率呼吸测定实验首次揭示了通过选择性树脂提取回收的OMWW酚类物质在防止氧化损伤后线粒体呼吸衰竭方面的有效性。收集到的数据明确地证明了我们的富酚组分的生物活性，支持了再利用橄榄厂废水的优势，以低成本，高附加值的分子，可以用于改善健康和营养产品。

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引用次数: 0

Integrated transcriptomic and proteomic analyses provide insights into the biosynthesis of Lycii fructus polysaccharides from different cultivation regions 综合转录组学和蛋白质组学分析提供了对不同栽培地区枸杞多糖生物合成的见解。

IF 4.1 Q2 FOOD SCIENCE & TECHNOLOGY

Food Chemistry Molecular Sciences

Pub Date : 2024-12-05 DOI: 10.1016/j.fochms.2024.100232

Limei Tong , Yinxiu Jiang , Xinrun Zhang , Xia Zhang , Yihao Zheng , Qiheng Zhao , Sheng Yu , Wenhua Zhang , Gang Ren , Zhanping Chen , Yuling Zhao , Sheng Guo , Hui Yan , Shulan Su , Yang Pan , Jin-ao Duan , Fang Zhang

Polysaccharides from L. fructus (LFPs) serve as important active biomacromolecules for the wide spectrum of bioactivities exhibited by Lycii fructus. However, the influence of ecological environments on the biosynthesis and structural characteristics of LFPs remains largely unexplored. The present research conducted a comprehensive strategy combining physicochemical structural elucidation, stoichiometric analysis, transcriptomic profiling, and proteomic analysis in L. fructus samples collected from typical cultivation regions including Ningxia, Xinjiang, and Qinghai. The results revealed distinct structural variations in LFPs from Ningxia compared to those from other regions in terms of Glc, Ara, GalA composition percentages as well as overall content. The omics data identified 5531 and 8084 differentially expressed genes (DEGs), as well as 3728 and 4732 differentially expressed proteins (DEPs) in Xinjiang and Qinghai compared to Ningxia, respectively. The integration of transcriptomic and proteomic analyses revealed enrichment of DEGs and DEPs involved in the biosynthesis pathway of LFPs, including UDPs, AXS, and UGDH. Temperature and precipitation were the significant ecological factors affecting LFPs biosynthesis. These comprehensive analyses provide a novel perspective for explaining the important material basis and environmental response mechanism underlying the quality formation of L. fructus.

枸杞子多糖（LFPs）是枸杞子具有广泛生物活性的重要生物大分子。然而，生态环境对lfp生物合成和结构特性的影响在很大程度上仍未被探索。本研究对宁夏、新疆、青海等典型栽培地区的枸杞子进行了理化结构解析、化学计量学分析、转录组学分析和蛋白质组学分析等综合研究。结果表明，与其他地区相比，宁夏lfp在Glc、Ara、GalA组成百分比和总体含量方面存在明显的结构差异。组学数据显示，新疆和青海与宁夏相比，分别鉴定出5531个和8084个差异表达基因（deg），以及3728个和4732个差异表达蛋白（dep）。转录组学和蛋白质组学的综合分析显示，参与LFPs生物合成途径的DEGs和DEPs富集，包括UDPs、AXS和UGDH。温度和降水是影响lfp生物合成的重要生态因子。这些综合分析为解释枸杞品质形成的重要物质基础和环境响应机制提供了新的视角。

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引用次数: 0

Metabolite differences and molecular mechanism between dehiscent and indehiscent capsule of mature sesame 成熟芝麻开裂与不开裂蒴果代谢差异及分子机制

IF 4.1 Q2 FOOD SCIENCE & TECHNOLOGY

Food Chemistry Molecular Sciences

Pub Date : 2024-11-26 DOI: 10.1016/j.fochms.2024.100231

Yinping Zhang , Ruirui Chen , Yujun Liu , Shuwen Xu , Shuguang Gao , Haiyang Zhang , Hongmei Miao , Lingling Qin , Xiangyu Zhou , Kiran Thakur , Cheng Li , Juan Li , Pengcheng Wei , Zhao-Jun Wei

The loss of sesame capsule seed prior to harvest poses a significant economical challenge in mechanized production. The metabolites involved in capsule closure are still unclear. Using comparative metabolome and transcriptome analysis, this work investigated the molecular regulation and enrichment pathways in two sesame types of indehiscent capsule WanZhi28 (ND) and dehiscent capsule WanZhi2 (WZ2). The findings demonstrated that genes and metabolites were significantly enriched in lignin synthesis-related pathways. Furthermore, data suggests that lipid and sugar metabolism may have an impact on capsule closure. Apart from its function in cell signaling, the latter may contribute to the glycosylation of lignin monomers, while the former may provide ATP for cellular microtubule movement. This work concurrently focused on a large number of differentially expressed transcription factors linked to the sesame capsule's anti-cleft mechanism, providing new evidence for the discovery and use of functional markers and genes for capsule dehiscence. The identification of key pathways and regulatory mechanisms offers valuable information for developing strategies to mitigate seed loss during harvest, ultimately contributing to more efficient and profitable sesame production.

芝麻蒴果在收获前的损失对机械化生产造成了重大的经济挑战。参与胶囊闭合的代谢物尚不清楚。采用比较代谢组学和转录组学分析方法，研究了芝麻两种类型灭裂胶囊万致28 （ND）和灭裂胶囊万致2 （WZ2）的分子调控和富集途径。结果表明，木质素合成相关通路的基因和代谢物显著富集。此外，数据表明脂质和糖代谢可能对胶囊闭合有影响。除了其在细胞信号传导中的功能外，后者可能有助于木质素单体的糖基化，而前者可能为细胞微管运动提供ATP。本工作同时关注了大量与芝麻荚膜抗裂机制相关的差异表达转录因子，为荚膜开裂功能标记和基因的发现和利用提供了新的证据。关键途径和调控机制的确定为制定减轻收获过程中种子损失的策略提供了有价值的信息，最终有助于提高芝麻生产的效率和利润。

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引用次数: 0

Calcium chloride connects potato greening and enzymatic browning through salicylic acid 氯化钙通过水杨酸将马铃薯变绿和酶促褐变联系起来

IF 4.1 Q2 FOOD SCIENCE & TECHNOLOGY

Food Chemistry Molecular Sciences

Pub Date : 2024-11-22 DOI: 10.1016/j.fochms.2024.100229

Jingkui Shi , Wenxin Xie , Yanmei Sun , Qingyu Shi , Xin Xing , Qingguo Wang , Qingqing Li

Greening and enzymatic browning are important factors causing post-harvest losses in potatoes. Although they are two different biological processes, there are some common inhibitors between them. Whether there is a correlation between the two has yet to be studies. In this research, we conducted transcriptome analysis of non-greening and greening potatoes, identifying several browning-related genes (polyphenol oxidase genes and peroxidase genes). Compared to non-greening potatoes, greening potatoes exhibited a greater browning degree. And calcium chloride (CaCl₂) can inhibit both greening and enzymatic browning. However, the inhibitory effect on potatoes was weakened when treated simultaneously with SA synthesis inhibitor and CaCl₂, indicating that CaCl₂ can regulate potato greening and browning by affecting internal SA synthesis. Additionally, exogenous SA treatment of potato tubers can also inhibit enzymatic browning. Our study not only demonstrated that CaCl₂ and SA can serve as a bridge connecting the potato greening and enzymatic browning, but also provided important references for the development of novel co-inhibitors.

变绿和酶促褐变是造成马铃薯收获后损失的重要因素。虽然它们是两个不同的生物过程，但它们之间存在一些共同的抑制因素。二者之间是否存在相关性还有待研究。在这项研究中，我们对非褐变马铃薯和褐变马铃薯进行了转录组分析，确定了几个与褐变相关的基因（多酚氧化酶基因和过氧化物酶基因）。与非褐变马铃薯相比，褐变马铃薯的褐变程度更高。而氯化钙（CaCl2）既能抑制褪绿，也能抑制酶促褐变。然而，同时使用 SA 合成抑制剂和 CaCl2 处理马铃薯时，其抑制作用减弱，这表明 CaCl2 可通过影响内部 SA 合成来调节马铃薯的褪绿和褐变。此外，外源 SA 处理马铃薯块茎也能抑制酶促褐变。我们的研究不仅证明了 CaCl2 和 SA 可作为连接马铃薯变绿和酶促褐变的桥梁，而且为开发新型协同抑制剂提供了重要参考。

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引用次数: 0

Metabonomic and transcriptomic profiling reveals amino acid metabolism affects the quality of premium japonica rice varieties in Northeast China 代谢组学和转录组学分析揭示氨基酸代谢对中国东北优质粳稻品种品质的影响

IF 4.1 Q2 FOOD SCIENCE & TECHNOLOGY

Food Chemistry Molecular Sciences

Pub Date : 2024-11-20 DOI: 10.1016/j.fochms.2024.100230

Jing Wang , Haitao Guan , Xiaolei Zhang , Changjun Dai , Cuiling Wang , Guofeng Chen , Kun Li , Zhenhua Xu , Ruiying Zhang , Baohai Liu , Hongtao Wen

Rice consumption and demand for premium rice are increasing worldwide. However, the characterizations and how to identify the premium rice are still unclear. Small molecular metabolites have a great advantage in distinguishing subtle differences among similar agricultural products. So, we hypothesized that the metabolites would be the key to identifying the tiny differences in premium rice among similar varieties. In this study, we performed metabolomic and transcriptomic profiles to comprehensively elucidate key metabolites, genes, and formation mechanisms of premium rice. As a result, eight compounds belong to four categories, and 49 different expressional genes were identified in premium rice varieties after comparing with the second-best varieties. Moreover, the integrated analysis confirmed that the amino acid pathway, including 42 expression genes and 11 metabolites, was critical for the premium rice formation. Six genes and two metabolites had significant regulatory effects on the pathways. Furthermore, amino acid quantification confirmed the content of 12 kinds of hydrolytic amino acids, such as aspartic acid and arginine were different between premium and other varieties. These amino acids may serve as potential biomarkers for differentiating premium rice in Northeast China. Our results strongly support the possibility of differentiating premium rice and would provide essential data for premium rice identification and metabolomics-assisted breeding.

全世界的大米消费量和对优质大米的需求都在不断增长。然而，优质大米的特征和鉴别方法仍不明确。小分子代谢物在区分同类农产品的细微差别方面具有很大优势。因此，我们假设代谢物将是识别优质大米在同类品种中微小差异的关键。在这项研究中，我们进行了代谢组学和转录组学分析，以全面阐明优质大米的关键代谢物、基因和形成机制。结果发现，与次优品种相比，优质水稻品种中的 8 种化合物分属 4 个类别，49 个表达基因各不相同。此外，综合分析证实，氨基酸途径（包括 42 个表达基因和 11 种代谢物）对优质稻米的形成至关重要。有 6 个基因和 2 个代谢物对该途径有显著的调控作用。此外，氨基酸定量分析证实，天门冬氨酸、精氨酸等 12 种水解氨基酸的含量在优质稻和其他品种之间存在差异。这些氨基酸可作为区分中国东北优质稻米的潜在生物标志物。我们的研究结果有力地支持了区分优质水稻的可能性，并将为优质水稻鉴定和代谢组学辅助育种提供重要数据。

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引用次数: 0

Characterization of the aroma and flavor profiles of guava fruit (Psidium guajava) during developing by HS-SPME-GC/MS and RNA sequencing 通过 HS-SPME-GC/MS 和 RNA 测序表征番石榴果实（Psidium guajava）发育过程中的香气和风味特征

IF 4.1 Q2 FOOD SCIENCE & TECHNOLOGY

Food Chemistry Molecular Sciences

Pub Date : 2024-11-06 DOI: 10.1016/j.fochms.2024.100228

Jie Zhang , Yi Zhang , Shuaiyu Zou , Endian Yang , Ziyi Lei , Tingting Xu , Chen Feng

The flavor of guava, an important tropical fruit, is influenced by secondary metabolites. However, the mechanisms and processes underlying flavor formation in guava remain unclear. In this study, dynamic changes in volatile organic compounds (VOCs), sugars, and organic acids in guava peel and flesh across different developmental stages were investigated using headspace solid-phase microextraction (HS-SPME) combined with gas chromatography-mass spectrometry (GC–MS) and high-performance liquid chromatography (HPLC). Here, we identified 90 VOCs, three sugars and eight organic acids. The dynamics of VOCs differ between the flesh and peel. The early developmental stages are more critical in influencing the variation of VOCs in the flesh, while VOC changes in peel occur more progressively across the developmental stages. By integrating transcriptomic and metabolomic analyses, we identified several key genes involved in VOC, sugar, and acid metabolism. This is the first study to describe the expression patterns of these genes throughout guava development, providing new insights into guava flavor development.

番石榴是一种重要的热带水果，其风味受次级代谢物的影响。然而，番石榴风味形成的机制和过程仍不清楚。本研究采用顶空固相微萃取（HS-SPME）结合气相色谱-质谱法（GC-MS）和高效液相色谱法（HPLC）研究了番石榴果皮和果肉中挥发性有机化合物（VOC）、糖类和有机酸在不同发育阶段的动态变化。在这里，我们确定了 90 种挥发性有机化合物、3 种糖和 8 种有机酸。果肉和果皮中的挥发性有机化合物动态各不相同。早期发育阶段对果肉中挥发性有机化合物的变化影响更为关键，而果皮中挥发性有机化合物的变化在各个发育阶段更为渐进。通过整合转录组和代谢组分析，我们确定了参与挥发性有机化合物、糖和酸代谢的几个关键基因。这是首次描述这些基因在番石榴整个发育过程中表达模式的研究，为番石榴风味的形成提供了新的见解。

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引用次数: 0

Study on dynamic alterations of volatile organic compounds reveals aroma development over enzymatic-catalyzed process of Tieguanyin oolong tea production 酶催化铁观音乌龙茶生产过程中挥发性有机化合物动态变化揭示香气发展的研究

IF 4.1 Q2 FOOD SCIENCE & TECHNOLOGY

Food Chemistry Molecular Sciences

Pub Date : 2024-10-16 DOI: 10.1016/j.fochms.2024.100227

Liangyu Wu , Xiaolan Chen , Jiaqi Lin , Hongzheng Lin , Ningkai Liao , Chenxue Li , Yunfei Hu , Yun Sun

To elucidate the formation of characteristic aroma over enzymatic-catalyzed processes (ECP), GC–MS-based volatile-metabolomic combined with desorption-electrospray-ionization coupled mass-spectrometry-imaging (DESI-MSI) were employed to analyze the changes of volatile organic compounds (VOCs) in Tieguanyin tea. A total of 579 VOCs were obtained, from which 24 components involved in five pathways were identified as biomarkers. Among these, four VOCs including 2-furancarboxylic acid, 4-methylbenzaldehyde, N-benzylformamide, cuminaldehyde, were detected in both DESI-MSI and GC–MS analysis, exhibiting dynamic changes along processing steps. RNA-sequencing analysis indicated the genes referring to stress response were activated during tea processing, facilitating the accumulation of flora-fruity aroma in tea leaf. Metabolic pathways analysis revealed that the increase in floral-fruity related components such as volatile terpenoids, phenylpropanoids/benzenoids, indole, alongside a decrease in green leaf volatiles including (E)-2-Hexenal, (Z)-3-Hexenol, played a crucial role in development of characteristic aroma, which could be a feasible index for evaluating processing techniques or quality of oolong tea.

为阐明酶催化过程（ECP）中特征香气的形成，采用基于气相色谱-质谱联用仪（GC-MS）的挥发性代谢组学和解吸-电喷雾电离耦合质谱-成像（DESI-MSI）分析了铁观音茶叶中挥发性有机化合物（VOCs）的变化。共获得 579 种挥发性有机化合物，并从中鉴定出涉及五种途径的 24 种成分作为生物标记物。其中，2-呋喃甲酸、4-甲基苯甲醛、N-苄基甲酰胺、积雪草醛等四种挥发性有机化合物在DESI-MSI和GC-MS分析中均被检测到，并表现出随加工步骤的动态变化。RNA 序列分析表明，应激反应基因在茶叶加工过程中被激活，促进了茶叶中花果香气的积累。代谢途径分析表明，花果香相关成分（如挥发性萜类化合物、苯丙酮类/类烯酮、吲哚）的增加，以及绿叶挥发物（包括(E)-2-己烯醛、(Z)-3-己烯醇）的减少，在特征香气的形成过程中发挥了关键作用。

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引用次数: 0

Waste to wealth: Polyhydroxyalkanoates (PHA) production from food waste for a sustainable packaging paradigm 变废为宝：利用食物垃圾生产聚羟基烷酸（PHA），打造可持续包装模式

IF 4.1 Q2 FOOD SCIENCE & TECHNOLOGY

Food Chemistry Molecular Sciences

Pub Date : 2024-10-10 DOI: 10.1016/j.fochms.2024.100225

Heri Septya Kusuma , Atna Sabita , Najla Anira Putri , Nadhira Azliza , Nafisa Illiyanasafa , Handoko Darmokoesoemo , Andrew Nosakhare Amenaghawon , Tonni Agustiono Kurniawan

The growing demand for sustainable food packaging and the increasing concerns regarding environmental pollution have driven interest in biodegradable materials. This paper presents an in-depth review of the production of Polyhydroxyalkanoates (PHA), a biodegradable polymer, from food waste. PHA-based bioplastics, particularly when derived from low-cost carbon sources such as volatile fatty acids (VFAs) and waste oils, offer a promising solution for reducing plastic waste and enhancing food packaging sustainability. Through optimization of microbial fermentation processes, PHA production can achieve significant efficiency improvements, with yields reaching up to 87 % PHA content under ideal conditions. This review highlights the technical advancements in using PHA for food packaging, emphasizing its biodegradability, biocompatibility, and potential to serve as a biodegradable alternative to petroleum-based plastics. However, challenges such as high production costs, mechanical limitations, and the need for scalability remain barriers to industrial adoption. The future of PHA in food packaging hinges on overcoming these challenges through further research and innovation in production techniques, material properties, and cost reduction strategies, along with necessary legislative support to promote widespread use.

人们对可持续食品包装的需求日益增长，对环境污染的关注也与日俱增，这促使人们对可生物降解材料产生了浓厚的兴趣。本文深入探讨了利用食物垃圾生产聚羟基烷酸酯（PHA）这种可生物降解的聚合物。以 PHA 为基础的生物塑料，尤其是从挥发性脂肪酸 (VFA) 和废油等低成本碳源中提炼出来的生物塑料，为减少塑料垃圾和提高食品包装的可持续性提供了一种前景广阔的解决方案。通过优化微生物发酵过程，PHA 的生产效率可显著提高，在理想条件下，PHA 的产量可达 87%。本综述重点介绍了将 PHA 用于食品包装的技术进展，强调了 PHA 的生物降解性、生物相容性以及作为石油基塑料的可生物降解替代品的潜力。然而，高昂的生产成本、机械限制和可扩展性需求等挑战仍然是工业应用的障碍。PHA 在食品包装中的未来取决于通过进一步研究和创新生产技术、材料特性和降低成本策略来克服这些挑战，同时还需要必要的立法支持来促进其广泛应用。

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引用次数: 0

The use of vitamin E in ocular health: Bridging omics approaches with Tocopherol and Tocotrienol in the management of glaucoma 维生素 E 在眼部健康中的应用：利用生育酚和生育三烯酚管理青光眼的全息方法

IF 4.1 Q2 FOOD SCIENCE & TECHNOLOGY

Food Chemistry Molecular Sciences

Pub Date : 2024-09-24 DOI: 10.1016/j.fochms.2024.100224

Fazira Latib Ratib, Muhamad Arif Irfan Zafendi, Mohd Aizuddin Mohd Lazaldin

Vitamin E, encompassing tocopherols and tocotrienols is celebrated for its powerful antioxidant properties, which help neutralize free radicals and protect cells from oxidative damage. Over the years, research has shown that both tocopherols and tocotrienols offer significant benefits, including protection against radiation damage, cholesterol regulation, cardiovascular health, and neurological disorders. This wide range of benefits highlights the need for further exploration of vitamin E’s role in managing various diseases. One particularly promising area is its potential application in treating ocular diseases like glaucoma. Despite advances in treatment, current options have limitations, making the investigation of alternative approaches crucial. Omics technologies, which allow for a detailed examination of biological systems, could provide valuable insights into how tocopherols and tocotrienols work at a molecular level. Their neuroprotective and antioxidative properties make them promising candidates for glaucoma management. Additionally, the sustainability of vitamin E is noteworthy, as by-products from its production can be repurposed into valuable resources for nutraceuticals and pharmaceuticals. As research continues, integrating omics technologies with the study of vitamin E derivatives could unveil new therapeutic possibilities, further enhancing our understanding of its diverse health benefits and its potential role in preventing and managing diseases.

维生素 E（包括生育酚和生育三烯酚）因其强大的抗氧化特性而备受赞誉，它有助于中和自由基，保护细胞免受氧化损伤。多年来的研究表明，生育酚和生育三烯酚都具有显著的益处，包括防止辐射损伤、调节胆固醇、保护心血管健康和神经系统疾病。这些广泛的益处突出表明，有必要进一步探索维生素 E 在控制各种疾病方面的作用。其中一个特别有前景的领域是维生素 E 在治疗青光眼等眼部疾病方面的潜在应用。尽管在治疗方面取得了进展，但目前的选择仍有局限性，因此研究替代方法至关重要。Omics 技术可以对生物系统进行详细检查，它可以为了解生育酚和生育三烯酚如何在分子水平上发挥作用提供有价值的见解。它们的神经保护和抗氧化特性使其成为治疗青光眼的理想候选药物。此外，维生素 E 的可持续性也值得注意，因为其生产过程中产生的副产品可以重新利用，成为营养保健品和药品的宝贵资源。随着研究的不断深入，将全息技术与维生素 E 衍生物的研究相结合，可能会揭示出新的治疗可能性，进一步加深我们对维生素 E 的各种健康益处及其在预防和控制疾病方面的潜在作用的了解。

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引用次数: 0

Combining widely targeted metabolomics and RNA-sequencing to reveal the function analysis of Phyllanthus emblica Linn. Juice-induced poultry macrophages 结合广泛的靶向代谢组学和RNA测序，揭示白花蛇舌草果汁诱导家禽巨噬细胞的功能分析果汁诱导的家禽巨噬细胞

IF 4.1 Q2 FOOD SCIENCE & TECHNOLOGY

Food Chemistry Molecular Sciences

Pub Date : 2024-09-22 DOI: 10.1016/j.fochms.2024.100223

Chenggang Liu, Jin jin, Binyi Sun

This study explored the functional effects of cultivated and wild Phyllanthus emblica Linn juice (PEJ) in HD11 poultry macrophage lines, with the aim of potentially developing cultivated PE and its fruit residue as poultry feed additives. RNA-Seq was used to evaluate the functional differences between cultivated and wild PEJ induced HD11 cells. Both cultivated and wild PEJ could regulate cell replication by histone H1/H2 family genes and host immune response by Toll-like receptor 7 regulation. Wild PEJ inhibited M1-type polarization of host macrophages, while cultivated PEJ promoted M2-type polarization. Metabolites of cultivated and wild PE were identified by widely targeted metabolomics based on liquid chromatography-tandem mass spectrometry. Of the 911 metabolites, 238 differed functionally between cultivated and wild PE. The data provide a theoretical basis for the subsequent development of PE as a functional feed additive in poultry.

本研究探讨了栽培的和野生的大叶黄杨果汁（PEJ）对HD11家禽巨噬细胞系的功能影响，目的是开发栽培的大叶黄杨及其果实残渣作为家禽饲料添加剂的潜力。RNA-Seq 用于评估栽培和野生 PEJ 诱导 HD11 细胞的功能差异。栽培PEJ和野生PEJ都能通过组蛋白H1/H2家族基因调控细胞复制，通过Toll样受体7调控宿主免疫反应。野生PEJ抑制宿主巨噬细胞的M1型极化，而栽培PEJ则促进M2型极化。基于液相色谱-串联质谱的广泛靶向代谢组学鉴定了栽培和野生 PE 的代谢物。在 911 个代谢物中，有 238 个在功能上存在差异。这些数据为后续开发 PE 作为家禽功能性饲料添加剂提供了理论依据。

引用次数: 0