EPPO Bulletin最新文献

This set continues the series of EPPO datasheets at present constituted by Quarantine Pests for Europe (2nd edition), the first supplementary set published in 1999 in the EPPO Bulletin issue 29, 459–506, the second supplementary set published in 2005 in issue 35, 361–475, the third supplementary set published in 2006 in issue 36, 1–9, the fourth supplementary set published in 2007 in issue 37, 223–245, the fifth, sixth and seventh supplementary sets published in 2008 in issue 38, 53–63, 161–166 and 439–449, respectively, the eighth and ninth supplementary sets published in issue 39, 41–64 and 487–499, the tenth supplementary set published in issue 40, 401–406, the eleventh supplementary set published in issue 41, 407–418, the twelfth and thirteenth supplementary sets published in issue 43, 139–143 and 195–196, the fourteenth, fifteenth and sixteenth supplementary sets published in issue 44, 5–10, 149–163 and 474–478, the seventeenth supplementary set published in issue 45, 2–13, the eighteenth supplementary set published in issue 46, 02–24, the nineteenth supplementary set published in issue 47, 524–548, the twentieth and twenty-first supplementary sets published in issue 49, 48–72 and 259–297, the twenty-second set published in issue 50, 156–169, the twenty-third set published in issue 50, 241–248, the 24th set published in issue 50, 526–548, the 25th set published in issue 52, 371–373, the 26th set published in issue 53, 100-107, the 27th set published in issue 53, 613–623, the 28th set published in issue 54, 25–40 and the 29th set published in issue 54, 343–360. It contains a further datasheet produced as part of the core work programme of EPPO datasheets.

EPPO Standard PM 3/29 (1) General export inspection procedures for glasshouse and nursery enterprises (EPPO, 1990) has been withdrawn. It is replaced by PM 3/99 (1) General guidelines for the export certification process for plants, plant products or other regulated articles (EPPO, 2025) which is published in this issue of the EPPO Bulletin.

PM 7/31 (2) Citrus tristeza virus, EPPO Bulletin, 53, 42–61 (2023). https://doi.org/10.1111/epp.12908

In the published version of the above article, Appendix 5 describes a real-time RT-PCR test adapted from Saponari et al. (2008). In Paragraph 2 ‘Methods’, a footnote under the Master Mix table specifies that the ‘iScript^™ reverse transcriptase Supermix (Biorad)’ is no longer commercially available. However, this applies to the ‘iScript^™ One-step RT-PCR kit for Probes (Biorad)’. The ‘iScript^™ reverse transcriptase Supermix (Biorad)’, originally provided with the kit, can be purchased separately. Therefore, the footnote is modified accordingly. The corrected version of Appendix 5 is shown below:

Appendix 5, paragraph 2.2

2.2 One-step real-time Reverse Transcription Polymerase Chain Reaction—real-time RT-PCR

2.2.1. Master Mix

2.2.2. Real-time RT-PCR cycling conditions: Reverse transcription at 55°C for 2 min, denaturation at 95°C for 5 min, 40 cycles of denaturation at 95°C for 15 s, and annealing and elongation at 59°C for 30 s.

In Appendix 7—RT-LAMP (Wang et al., 2013), in the Master Mix table, the ref. code ‘ISO-DR004’, was included for the ‘Isothermal Master Mix (Optigene)’. However, the correct code is ‘ISO-DR004-RT’. The corrected version of Appendix 7 is shown below:

Appendix 7, paragraph 2.2

2.2. One-step RT-LAMP

2.2.1. Master Mix

2.2.2. RT-LAMP conditions: 65°C for 45 min, 98–80°C, ramping at 0.1°C per s.

This work documents the first occurrence of Etiella zinckenella (Lepidoptera: Pyralidae) infestations in soybean crops in Greece, and in particular within the Regional Unit of Drama, north-eastern Greece. Larvae of this pest inflict direct damage by feeding on seeds and cause indirect damage by reducing crop marketability, resulting in significant yield and economic losses on different hosts worldwide. Additionally, this report introduces Dolichogenidea appellator (Hymenoptera: Braconidae), a parasitoid of E. zinckenella, found in infested soybean pods, that has been identified using DNA barcoding and is documented for the first time in Greece. Given the substantial economic implications and the necessity for effective integrated pest management practices, further research is essential to elucidate the interactions between environmental conditions, pest dynamics and agricultural practices in the region. This study provides a critical foundation for future investigations into soybean pest management in Greece.