EPPO Bulletin最新文献

This set continues the series of EPPO datasheets at present constituted by Quarantine Pests for Europe (2nd edition), the first supplementary set published in 1999 in the EPPO Bulletin issue 29, 459–506, the second supplementary set published in 2005 in issue 35, 361–475, the third supplementary set published in 2006 in issue 36, 1–9, the fourth supplementary set published in 2007 in issue 37, 223–245, the fifth, sixth and seventh supplementary sets published in 2008 in issue 38, 53–63, 161–166 and 439–449, respectively, the eighth and ninth supplementary sets published in issue 39, 41–64 and 487–499, the tenth supplementary set published in issue 40, 401–406, the eleventh supplementary set published in issue 41, 407–418, the twelfth and thirteenth supplementary sets published in issue 43, 139–143 and 195–196, the fourteenth, fifteenth and sixteenth supplementary sets published in issue 44, 5–10, 149–163 and 474–478, the seventeenth supplementary set published in issue 45, 2–13, the eighteenth supplementary set published in issue 46, 02–24, the nineteenth supplementary set published in issue 47, 524–548, the twentieth and twenty-first supplementary sets published in issue 49, 48–72 and 259–297, the twenty-second set published in issue 50, 156-169, the twenty-third set published in issue 50, 241-248, the 24th set published in issue 50, 526-548, the 25th set published in issue 52, 371-373, the 26th set published in issue 53, 100-107 and the 27th set published in issue 53, 613-623. It contains 2 further datasheets produced as part of the core work programme of EPPO datasheets as well as information on the new progress made in the datasheets project.

Phenacoccus solenopsis Tinsley, 1898 (Hemiptera: Pseudococcidae), commonly known as the cotton mealybug or solenopsis mealybug, is a highly polyphagous species. This paper provides the first report of this species in Greece. It was detected in high numbers on tomato (Solanum lycopersicum) plants cultivated in a greenhouse in the region of Mirtos, close to the city of Ierapetra in the Southeast part of the island of Crete. The species identification was carried out using morphological and molecular analyses. The fact that P. solenopsis can be transferred easily via trade of live plant material, in combination with its ability to affect many different hosts causing severe damage, constitutes an increasing threat to Greek cultivation and especially to horticultural and vegetable crops. Additional surveys should be conducted to investigate the species distribution in Greece.

Ripersiella hibisci (Kawai & Takagi, 1971) is a root mealybug included in the EPPO A1 list of pests recommended for regulation and an EU A1 Quarantine pest list (Annex IIA). This pest causes damage to several species of monocotyledonous and dicotyledonous ornamental and foliage potted plants, especially bonsais. The underground location of R. hibisci makes its detection difficult during phytosanitary controls, and moreover, it can be confused with other non-regulated species of mealybugs (e.g. Planococcus citri, P. ficus, Rhizoecus falcifer, Rhizoecus dianthi). Thus, a molecular test based on real-time PCR was designed for rapid and unambiguous identification of this mealybug. Analytical sensitivity, analytical specificity, repeatability and reproducibility of the test were assessed as part of the validation. The test showed 100% analytical specificity (exclusivity and inclusivity), allowing the target to be distinguished from all non-target species tested (exclusivity); the inclusivity was demonstrated, validating the test on different populations of the pest. The molecular test is an efficient tool for the identification of the root mealybug R. hibisci (in its various life stages), which is particularly useful when a rapid screening of samples is needed to limit or avoid the introduction of the pest.

Following a Proficiency Test organized by the European Union Reference Laboratory (EURL) for Viruses, Viroids and Phytoplasmas on chrysanthemum stem necrosis virus (CSNV), it has been brought to the attention of the EPPO Secretariat that the conventional RT-PCR test from Hassani-Mehraban et al. (2016), described in Appendix 4, of the EPPO Standard PM 7/139 (1) Tospoviruses (Genus Orthotospovirus) (EPPO, 2020) was not able to reliably detect CSNV.

The validation data produced by the EURL for Viruses, Viroids and Phytoplasmas will be considered during the revision of Annex 4 of the EPPO Diagnostic Protocol PM 7/139 Tospoviruses (Genus Orthotospovirus). The revision of the Diagnostic Protocol is currently in progress.

In the coastal area of North-Western Morocco, 43 sites in four provinces were surveyed between spring 2022 and autumn 2023 for the presence of pest scale insects (Hemiptera: Coccomorpha) on citrus trees. A known citrus pest species, pyriform scale, Protopulvinaria pyriformis (Cockerell, 1894) (Coccidae), was found at four sites. This is the first paper reporting pyriform scale on citrus in Morocco so a species description based on 62 slide-mounted adult females is provided. Currently, P. pyriformis is not causing damage on citrus in Morocco, but potentially it might become a pest on this crop or other commercial fruit crops, such as avocado. Populations of P. pyriformis therefore should be monitored to detect any spread of the scale to other areas and crops. The infestations were found to be associated with a predator Chilocorus bipustulatus (Linnaeus) (Coleoptera: Coccinellidae) and two parasitic wasps: an ectoparasitoid, Aphytis sp. (Hymenoptera: Aphelinidae), and an unidentified endoparasitoid (Hymenoptera: Chalcidoidea).

The glasshouse leafhopper, Hauptidia maroccana (Melichar, 1907), is recorded for the first time in Jordan. It was found infesting basil, tomato, pepper, peppermint, celery and parsley plants in ‘Ammān in October 2023. Adult genitalia slide mounts were prepared and voucher specimens were preserved at the University of Jordan Insects Museum. Original images for nymph, adult male and female genitalia, and distribution data, are provided.