{"title":"Rapport annuel et recommandations du Conseil pour 2024","authors":"","doi":"10.1111/epp.70037","DOIUrl":"https://doi.org/10.1111/epp.70037","url":null,"abstract":"","PeriodicalId":34952,"journal":{"name":"EPPO Bulletin","volume":"55 3","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-11-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145772534","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"PM 3/70 (2) Compliance procedures for consignments of potato tubers at export and import","authors":"","doi":"10.1111/epp.70030","DOIUrl":"https://doi.org/10.1111/epp.70030","url":null,"abstract":"","PeriodicalId":34952,"journal":{"name":"EPPO Bulletin","volume":"55 3","pages":"342-387"},"PeriodicalIF":0.0,"publicationDate":"2025-10-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145772754","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
I. Mrabti, H. Benzahra, H. Grijja, M. El Ammari, F. El Bahja, N. Brhadda, M. Afechtal, R. Ziri
A study of soft scale insects (Hemiptera: Coccidae) was conducted on a wide range of plants between May 2021 and September 2024 along the north-west coast of Morocco, covering 110 sites across 10 provinces. This led to morphological and molecular identification of hemispherical scale, Saissetia coffeae (Walker), from Moroccan specimens. Although this species had previously been reported from Morocco, based on morphological characters, the present study documents the occurrence of this species on various host plants in Morocco for the first time, including citrus (Citrus spp.), avocado (Persea americana Mill.), edible fig (Ficus carica L.), strangler fig (Ficus retusa L.) and sago palm (Cycas revoluta Thunb.), at eight locations across the country. The research also highlights the crucial role of natural enemies in the natural regulation of S. coffeae populations. Three species of endoparasitoid wasps (Hymenoptera) were identified: Metaphycus flavus and Coccophagus sp. (Encyrtidae) and Scutellista caerulea (Pteromalidae). In addition, seven species of predators were found: Chilocorus renipustulatus (L.), Scymnus syriacus (Marseul), Stethorus pusillus (Herbst) and Exochomus flavipes (Thunberg) (Coleoptera: Coccinellidae), Orius laevigatus (Fieber) (Hemiptera: Anthocoridae) and Chrysoperla carnea (Stephens) (Neuroptera: Chrysopidae) and Eublemma scitula (Rambur) (Lepidoptera: Crambidae).
{"title":"Saissetia coffeae (Walker, 1852) (Hemiptera: Coccidae): An emerging pest on various crops and ornamental plants in Morocco","authors":"I. Mrabti, H. Benzahra, H. Grijja, M. El Ammari, F. El Bahja, N. Brhadda, M. Afechtal, R. Ziri","doi":"10.1111/epp.70029","DOIUrl":"https://doi.org/10.1111/epp.70029","url":null,"abstract":"<p>A study of soft scale insects (Hemiptera: Coccidae) was conducted on a wide range of plants between May 2021 and September 2024 along the north-west coast of Morocco, covering 110 sites across 10 provinces. This led to morphological and molecular identification of hemispherical scale, <i>Saissetia coffeae</i> (Walker), from Moroccan specimens. Although this species had previously been reported from Morocco, based on morphological characters, the present study documents the occurrence of this species on various host plants in Morocco for the first time, including citrus (<i>Citrus</i> spp.), avocado (<i>Persea americana</i> Mill.), edible fig (<i>Ficus carica</i> L.), strangler fig (<i>Ficus retusa</i> L.) and sago palm (<i>Cycas revoluta</i> Thunb.), at eight locations across the country. The research also highlights the crucial role of natural enemies in the natural regulation of <i>S. coffeae</i> populations. Three species of endoparasitoid wasps (Hymenoptera) were identified: <i>Metaphycus flavus</i> and <i>Coccophagus</i> sp. (Encyrtidae) and <i>Scutellista caerulea</i> (Pteromalidae). In addition, seven species of predators were found: <i>Chilocorus renipustulatus</i> (L.), <i>Scymnus syriacus</i> (Marseul), <i>Stethorus pusillus</i> (Herbst) and <i>Exochomus flavipes</i> (Thunberg) (Coleoptera: Coccinellidae), <i>Orius laevigatus</i> (Fieber) (Hemiptera: Anthocoridae) and <i>Chrysoperla carnea</i> (Stephens) (Neuroptera: Chrysopidae) and <i>Eublemma scitula</i> (Rambur) (Lepidoptera: Crambidae).</p>","PeriodicalId":34952,"journal":{"name":"EPPO Bulletin","volume":"55 3","pages":"476-486"},"PeriodicalIF":0.0,"publicationDate":"2025-10-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145766401","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"PM 7/122 (3) Guidelines for the organization of interlaboratory comparisons by plant pest diagnostic laboratories","authors":"","doi":"10.1111/epp.70033","DOIUrl":"https://doi.org/10.1111/epp.70033","url":null,"abstract":"","PeriodicalId":34952,"journal":{"name":"EPPO Bulletin","volume":"55 3","pages":"443-457"},"PeriodicalIF":0.0,"publicationDate":"2025-10-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145772629","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
The EPPO Panel on Diagnostics in Nematology agreed to add the three recently described species (X. babaii, X. persicum and X. purpureum), which have not been reported to vector plant viruses, to the polytomous key tables in the EPPO Standard PM 7/95 (2) Xiphinema americanumsensu lato. This implies minor updates to the key (Tables 2 and 4) and Appendix 1.
The new lines to be added to Tables 2 and 4 and to the Table of Appendix 1 are shown in bold below.
{"title":"Addendum (2) – PM 7/95 (2) Xiphinema americanum sensu lato","authors":"","doi":"10.1111/epp.70035","DOIUrl":"https://doi.org/10.1111/epp.70035","url":null,"abstract":"<p>The EPPO Panel on Diagnostics in Nematology agreed to add the three recently described species (<i>X. babaii</i>, <i>X. persicum</i> and <i>X. purpureum</i>), which have not been reported to vector plant viruses, to the polytomous key tables in the EPPO Standard PM 7/95 (2) <i>Xiphinema americanum</i> <i>sensu lato</i>. This implies minor updates to the key (Tables 2 and 4) and Appendix 1.</p><p>The new lines to be added to Tables 2 and 4 and to the Table of Appendix 1 are shown in bold below.</p>","PeriodicalId":34952,"journal":{"name":"EPPO Bulletin","volume":"55 3","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-10-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/epp.70035","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145772482","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
In EPPO Standard PM 7/110 (2), the EPPO Code given for Xanthomonas vesicatoria in the identity section was incorrect. The correct EPPO Code for Xanthomonas vesicatoria is XANTVE.
Deniz Çaplık, Feyzullah Yılmaz, Osman Çiftçi, Mustafa Küsek, Mehmet Ertuğrul Güldür, Şahimerdan Türkölmez, Gülten Nisan Ozan
Pistachio (Pistacia vera L.) is a vital crop for Türkiye's economy, occupying over 420 000 ha, primarily in south-eastern Anatolia. In 2023, severe symptoms, such as curling, chlorosis and needle-like leaf deformation, were observed on pistachio trees in Şanlıurfa Province. This prompted a molecular study of phytoplasma pathogens. A real-time PCR targeting the 16SrDNA gene was used to analyse DNA from eight symptomatic and two asymptomatic trees. A phytoplasma was found in two symptomatic samples but not in asymptomatic controls. Positive samples were further analysed using nested PCR. One sequence with 99% identity to ‘Candidatus Phytoplasma trifolii’ (16SrVI-A subgroup) was found by bidirectional sequencing of PCR products. The identification of the phytoplasma was confirmed by a phylogenetic analysis showing the clustering of the UP 1 isolate with ‘Ca. Phytoplasma trifolii’ reference strain and by a virtual RFLP analysis using 17 restrictions enzymes. This first report of ‘Ca. Phytoplasma trifolii’ in pistachio in Türkiye highlights the presence of this phytoplasma in a key agricultural sector. Although no yield losses have been observed to date, broader surveys and monitoring of leafhopper vectors are necessary to assess the potential risk and implement timely management strategies.
开心果(Pistacia vera L.)是土耳其经济的重要作物,占地超过42万公顷,主要分布在安纳托利亚东南部。2023年,在Şanlıurfa省的开心果树上发现了卷曲、褪绿和针状叶变形等严重症状。这促使了植物原体病原体的分子研究。采用实时荧光定量PCR技术对8株有症状树和2株无症状树的16SrDNA基因进行分析。在两个有症状的样本中发现了植物原体,但在无症状的对照组中未发现。阳性样本进一步采用巢式PCR分析。对PCR产物进行双向测序,发现1个序列与‘Candidatus Phytoplasma trifolii’(16SrVI-A亚群)具有99%的同源性。系统发育分析表明,该植物原体与‘Ca’具有聚类性。利用17种限制性内切酶进行虚拟RFLP分析。这是“Ca”的第一份报告。斯里兰卡开心果中的“三叶植原体”突显了该植原体在关键农业部门的存在。虽然到目前为止尚未观察到产量损失,但有必要对叶蝉病媒进行更广泛的调查和监测,以评估潜在风险并及时实施管理策略。
{"title":"First detection of ‘Candidatus Phytoplasma trifolii’ in pistachio trees in Türkiye: A new and emerging threat","authors":"Deniz Çaplık, Feyzullah Yılmaz, Osman Çiftçi, Mustafa Küsek, Mehmet Ertuğrul Güldür, Şahimerdan Türkölmez, Gülten Nisan Ozan","doi":"10.1111/epp.70009","DOIUrl":"https://doi.org/10.1111/epp.70009","url":null,"abstract":"<p>Pistachio (<i>Pistacia vera</i> L.) is a vital crop for Türkiye's economy, occupying over 420 000 ha, primarily in south-eastern Anatolia. In 2023, severe symptoms, such as curling, chlorosis and needle-like leaf deformation, were observed on pistachio trees in Şanlıurfa Province. This prompted a molecular study of phytoplasma pathogens. A real-time PCR targeting the 16SrDNA gene was used to analyse DNA from eight symptomatic and two asymptomatic trees. A phytoplasma was found in two symptomatic samples but not in asymptomatic controls. Positive samples were further analysed using nested PCR. One sequence with 99% identity to ‘<i>Candidatus</i> Phytoplasma trifolii’ (16SrVI-A subgroup) was found by bidirectional sequencing of PCR products. The identification of the phytoplasma was confirmed by a phylogenetic analysis showing the clustering of the UP 1 isolate with ‘<i>Ca.</i> Phytoplasma trifolii’ reference strain and by a virtual RFLP analysis using 17 restrictions enzymes. This first report of ‘<i>Ca.</i> Phytoplasma trifolii’ in pistachio in Türkiye highlights the presence of this phytoplasma in a key agricultural sector. Although no yield losses have been observed to date, broader surveys and monitoring of leafhopper vectors are necessary to assess the potential risk and implement timely management strategies.</p>","PeriodicalId":34952,"journal":{"name":"EPPO Bulletin","volume":"55 3","pages":"487-493"},"PeriodicalIF":0.0,"publicationDate":"2025-10-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145772614","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}