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Isolation and Identification of Local Soil Bacteria with Larvicidal Activities Against Aedes aegypti Larvae 对埃及伊蚊幼虫具有杀幼虫活性的地方土壤细菌的分离鉴定
Q3 Agricultural and Biological Sciences Pub Date : 2022-04-01 DOI: 10.3844/ojbsci.2022.520.528
N. Alotaibi, S. Saïdi, M. Roushdy, Idris Abdulrahman, A. Saddiq, Turki M. Alshaikh, Naser AL- Kenani, Saleh Mohammed Al Maaqar
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引用次数: 0
Effective Strains of Beauveria Bassiana and B. Pseudobassiana used Against the Asian Locust (Locusta Migratoria L.) in Kazakhstan 球孢白僵菌和假球孢白僵菌在哈萨克斯坦防治亚洲蝗虫的有效菌株
Q3 Agricultural and Biological Sciences Pub Date : 2022-04-01 DOI: 10.3844/ojbsci.2022.492.501
Ulzhalgas T. Zhumataуeva, Bakhytzhan A. Duissembekov, Yerlan Bozanbaiulu Dutbayev, K. Kidirbayeva, O. A. Alshynbayev, G. A. Bekbulatova
: The purpose of the study was to perform genetic identification of the strains of fungi of the Beauveria genus and evaluate the effect of abiotic factors on the growth and survival of colonies and the productivity of conidia of these fungi, as well as to determine the biological effectiveness of the semi-preparative form of the drug based on Beauveria bassiana in the field. Conventional and modern methods accepted in biology and mycology were used. Identification of strains of Beauveria fungi was carried out by determining the direct nucleotide sequence of the TEF region. Laboratory experiments were carried out to evaluate various factors on the diameter of colony growth, survival in deep and surface cultivation, the type of semi-preparative form of the drug based on B. bassiana and the time of its exposure, and the effect on the biological effects of drugs in the field. Statistical data processing was carried out in the R studio program using the P value. The productivity of conidia of entomopathogenic fungi during surface cultivation depended on the type of strain and the duration of cultivation of conidia. The diameter of the growth of Beauveria fungi colonies was influenced by the factors of the strain, the type of nutrient medium, the temperature of colony cultivation, and the number of days after the start of colony cultivation. The survival of conidia of the B. bassiana fungus on the wings of Locusta migratoria L. orrelated with the indicators of cultivation temperature and germination of conidia and cultivation time. The productivity of Beauveria spp. conidia correlate with the type of strain, the type of substrate, and the time of exposure. The conidia continued to germinate on the 25 th day. Fungi grew better on a substrate based on millet. In the field, the mortality rate of L. migratoria L. and the biological effectiveness of biopesticides were influenced by the factors of the strain, the form of spraying with the bio preparation, and the time elapsed after treatment with the biopesticide.
本研究的目的是对白僵菌属真菌菌株进行遗传鉴定,评价非生物因素对真菌菌落生长、存活及分生孢子产量的影响,并确定以白僵菌为基础的药物半制剂在田间的生物学有效性。采用了生物学和真菌学中公认的传统和现代方法。通过测定TEF区直接核苷酸序列对白僵菌进行鉴定。通过室内试验,考察了菌落生长直径、深耕和浅耕存活率、以球孢白僵菌为原料的药物半制剂类型和暴露时间等因素对药物田间生物效应的影响。在R studio程序中使用P值对统计数据进行处理。昆虫病原真菌在地表培养过程中分生孢子的产量取决于菌株类型和分生孢子的培养时间。白僵菌菌落生长直径受菌种、营养培养基类型、菌落培养温度、菌落培养开始天数等因素的影响。球孢白僵菌在飞蝗翅上的分生孢子存活率与培养温度、分生孢子萌发和培养时间有关。分生白僵菌的产率与菌株类型、底物类型和暴露时间有关。第25天分生孢子继续萌发。真菌在以谷子为基材的基质上生长得更好。在田间,影响迁徙乳杆菌死亡率和生物农药生物药效的因素主要有菌种、生物制剂的喷洒方式和施药时间。
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引用次数: 1
Water Quality in Small-Scale Coffee Production Units, Amazonas, Peru 秘鲁亚马逊地区小型咖啡生产单位的水质
Q3 Agricultural and Biological Sciences Pub Date : 2022-04-01 DOI: 10.3844/ojbsci.2022.448.455
E. Rojas, S. Quintana, Magali García, J. Veneros, M. Oliva, José Carlos Santa Cruz Guerrero, M. Pino, A. Guadalupe, T. S. Santillan
: The objective of this research was to determine water quality in small-scale coffee production units in the Amazon Region, Peru. The characterization of the factors associated with coffee farmers was collected through surveys. The Standard Methods for the Examination of Water and Wastewater (APHA) method was used to determine the physicochemical and microbiological parameters of the incoming water (AE) (water for human consumption) and the outgoing water (AS) (wastewater from coffee washing). The results indicated that the coffee growers do not have adequate technology for washing the coffee and that they use water for these activities. In the characterization of the water, significant differences were found between the parameters of the AE and the AS, where the pH of the AE ranged from 7.00 to 7.32 and the pH of the AS from 3.76 to 4.44. The turbidity of the AS showed high values of 1814.47 NTU. Total Coliforms (TC) and heavy metals such as copper and chromium all increased in value up to 0.20 and 0.15 ppm in the AS compared to the AE. The characteristics of the water quality consumed by the coffee growers are poor and values above Peruvian standards were found.
:本研究的目的是确定秘鲁亚马逊地区小型咖啡生产单位的水质。通过调查收集了与咖啡种植者相关的因素特征。采用《水和废水检验标准方法》(APHA)方法测定进水(AE)(人类饮用水)和出水(AS)(咖啡洗涤废水)的物理化学和微生物参数。结果表明,咖啡种植者没有足够的技术来清洗咖啡,他们在这些活动中使用水。在水的表征中,发现AE和AS的参数之间存在显著差异,其中AE的pH在7.00到7.32之间,AS的pH在3.76到4.44之间。AS的浊度显示出1814.47NTU的高值。与AE相比,as中的总大肠杆菌(TC)和重金属(如铜和铬)的值均增加了0.20和0.15ppm。咖啡种植者饮用的水质特征较差,其值高于秘鲁标准。
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引用次数: 0
Pasture Productivity Depending on the Method of Pasture use in the Steppe Zone of Northern Kazakhstan 哈萨克斯坦北部草原区牧草利用方式对草地生产力的影响
Q3 Agricultural and Biological Sciences Pub Date : 2022-04-01 DOI: 10.3844/ojbsci.2022.476.483
Balzhan Akhylbekova, N. Serekpayev, Adilbek Nogayev, Bakhytbek Zhumabek
: Unsystematic grazing of animals and continuous use of pastures leads to their degradation and, accordingly, to a shortage of pasture forage for the production of competitive environmentally friendly livestock products. The proportion of productive grasses in grass covers is decreasing; therefore, there is an urgent need to preserve valuable plant species in pasture grass, restore the productivity of natural vegetation and enrich it with valuable grasses. This study evaluated the impact of the unsystematic grazing method and a developed pasture rotation scheme on the height of the herbage and the productivity of seasonal pasture plots. The study was conducted as an experiment in the Arshaly district of the Akmola region located in the northern part of Kazakhstan. In 2019, unsystematic grazing of animals was carried out on the studied contours of the pasture. The animals grazed without alternating pastures and, as a result, the plants were subjected to intensive grazing for long periods or without sufficient recovery periods. In 2020, there was rotational grazing, where cattle grazed in different areas according to the seasons using a certain pasture rotation. As a result of the conducted study, it was found that with pasture rotation, the height of the herbage increased from 9.56±2.94 to 16.42±5.65 cm. The productivity of pastures after grazing decreased sharply but in the following season, the grassland recovered from 0.77±0.22 to 0.91±0.4 t/ha and exceeded the indicators of unsystematic grazing.
:对动物的不系统放牧和牧场的持续使用导致了它们的退化,因此导致了生产有竞争力的环境友好型畜牧产品的牧草短缺。可生产草在草地覆盖中的比例正在下降;因此,迫切需要保护牧草中有价值的植物物种,恢复自然植被的生产力,并用有价值的草来丰富它。本研究评估了非系统放牧方法和制定的牧场轮作方案对季节性牧场牧草高度和生产力的影响。这项研究是在哈萨克斯坦北部阿克莫拉地区的阿尔沙利区进行的一项实验。2019年,在研究的牧场轮廓上进行了非系统的动物放牧。这些动物在没有交替牧场的情况下放牧,因此,这些植物被长期集中放牧,或者没有足够的恢复期。2020年,实行了轮牧,牛根据季节在不同的地区放牧,采用一定的牧场轮牧方式。研究结果表明,随着牧场轮作,牧草高度从9.56±2.94厘米增加到16.42±5.65厘米。放牧后牧场生产力急剧下降,但在下一个季节,草地从0.77±0.22吨/公顷恢复到0.91±0.4吨/公顷,超过了非系统放牧的指标。
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引用次数: 2
Prevention of Desertification and Land Degradation using Black Saxaul in Arid Conditions 在干旱条件下利用黑撒克逊树预防荒漠化和土地退化
Q3 Agricultural and Biological Sciences Pub Date : 2022-04-01 DOI: 10.3844/ojbsci.2022.484.491
Birzhan Yessimbek, B. Mambetov, R. Akhmetov, D. Dosmanbetov, K. Abayeva, Ardak Kozhabekova, A. Oraikhanova, M. Baibatshanov
: The harsh climate of the arid zone determines several biological features in black saxaul plantations. Therefore, the study of the growth and condition of artificial plantations, depending on the use of physiologically active moisture-absorbing substances and growth biostimulants, is of particular practical importance. In the Moinkum sands of the Zhambyl region (Kazakhstan), observations of the growth and development of the black saxaul in places where physiologically active substances and biostimulants of growth were used were continued at industrial plantings in 2018, 2019, and 2020. The purpose of these studies was to prevent desertification by improving the preservation of forest crops of the black saxaul. The use of various water-retaining compounds (hydrogels), growth stimulants (Epin-Extra), and fertilizers, as well as their combined use, had a positive effect. The use of moisture-absorbing substances and biostimulators of plant growth in the cultivation of black saxaul increased the average diameter and average height of seedlings by an average of 30.1 and 38.6%, respectively. The best effect of the growth stimulators used was shown with hydrogel, Epin-Extra (plant growth biostimulator), and a ready-made mixture of hydrogel, fertilizer, and Epin. The use of moisture-absorbing substances, growth biostimulators, and nitrogen fertilizers affected the second and subsequent years after planting.
:干旱区的恶劣气候决定了黑沙索人工林的几个生物学特征。因此,根据生理活性吸湿物质和生长生物刺激剂的使用,研究人工林的生长和条件具有特别重要的现实意义。在扎姆比尔地区(哈萨克斯坦)的Moinkum沙地,2018年、2019年和2020年的工业种植中,继续观察了使用生理活性物质和生长生物刺激剂的地方黑沙索的生长和发育。这些研究的目的是通过改善黑沙索尔森林作物的保护来防止荒漠化。各种保水化合物(水凝胶)、生长刺激剂(Epin Extra)和肥料的使用以及它们的联合使用产生了积极影响。在黑沙索的栽培中使用吸湿物质和植物生长的生物刺激剂,使幼苗的平均直径和平均高度分别平均增加30.1%和38.6%。使用的生长刺激剂的最佳效果显示为水凝胶、Epin Extra(植物生长生物刺激剂)以及水凝胶、肥料和Epin的现成混合物。吸湿物质、生长生物刺激剂和氮肥的使用影响了种植后的第二年和随后的几年。
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引用次数: 7
Milk Content and Milk Quality of Sheep of the Kazakh Fat-Tailed Semi-Rough-Haired Breed with Pasture Content 哈萨克粗尾半毛羊产奶量及乳品质与牧草含量的关系
Q3 Agricultural and Biological Sciences Pub Date : 2022-04-01 DOI: 10.3844/ojbsci.2022.469.475
D. Ibrayev, S. Shauyenov, G. Zhakupova, Narzhan Zhumadillayev, A. Muldasheva
: The article presents the results of a study on the effect of top dressing and feed additives based on humic substances on milk productivity, the quality of milk of sheep of the Kazakh fat-tailed semi-coarse breed, and the resulting cheese in pasture conditions. In total, 90 heads of sheep of the Kazakh short-tailed semi-rough-haired breed were used in the experiment. The Control Group (CG) of 30 sheep grazed on pasture grass, while two Experimental Groups (EG) of 60 heads were fed 300 g barley per day of barley, The second experimental group of sheep received a feed additive based on humic substances. As a result, after the first month of feeding, additional feeding had a positive effect on the daily milk yield of ewes, where the experimental group II was statistically significantly superior (P<0.05), i.e., it exceeded by 0.08 liters. In total, during lactation, the sheep of the second experimental group exceeded the control group by an average of 10.8% in average daily milk yield. Also, additional feeding affected the quality of milk and cheese, Thus, the indicators of sheep of the II experimental group were superior in terms of the fat content of milk (P<0.01) and cheese (P<0.05).
本文介绍了在牧场条件下,基于腐殖物质的追肥和饲料添加剂对哈萨克肥尾半粗羊产奶量、产奶质量以及由此生产的奶酪的影响的研究结果。实验总共使用了90头哈萨克短尾半粗毛羊。对照组(CG)30只羊在牧草上放牧,两个实验组(EG)60头每天喂300克大麦。第二个实验组的羊接受基于腐殖物质的饲料添加剂。因此,在喂养第一个月后,额外喂养对母羊的日产奶量有积极影响,其中实验组II在统计学上显著优于实验组(P<0.05),即超过0.08升。总的来说,在哺乳期,第二实验组的绵羊平均日产奶量超过对照组10.8%。此外,添加饲料也会影响牛奶和奶酪的质量。因此,II实验组绵羊的各项指标在牛奶脂肪含量(P<0.01)和奶酪脂肪含量(P<0.05)方面均优于II实验组。
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引用次数: 0
Evaluation of the Efficacy of Entolek K Planteco® Biopesticide Based on Akanthomyces Lecanii Fungus Against Pest Orthopterans in Soybean Agroecosystems in Southeast Kazakhstan 基于Akanthomyces Lecanii真菌的Entolek K Planteco®生物农药对哈萨克斯坦东南部大豆农业生态系统害虫直翅虫的防治效果评价
Q3 Agricultural and Biological Sciences Pub Date : 2022-04-01 DOI: 10.3844/ojbsci.2022.502.511
I. Temreshev, A. Tursynkulov, Yerlan Bozanbaiulu Dutbayev, A. Makezhanov, G. Suleimanova
: Pest control services dealing with mass pests such as locusts and grasshoppers are forced to use insecticides of various origins to prevent crop damage. In most cases, mainly chemical insecticides are used. This method of regulating pest populations has many negative consequences. As a result of chemical treatments with broad-spectrum insecticides, not only pests are killed, but also non–target organisms, primarily entomophages and pollinators. One of the alternatives to the chemical method of control is the use of biopesticides based on entomopathogenic viruses, bacteria, and fungi. The impact of the biopesticide "Entolek K Planteco®" based on the fungus Akanthomyces lecanii on different species of locusts, grasshoppers, and katydids was evaluated in soybean plantings of southeast Kazakhstan. Locust and grasshopper mortality was 70% on the 3 rd day after treatment and 98% on the 7 th day, while katydid mortality on the same days was 50 and 70% respectively. Thus "Entolek K Planteco ®" proved efficient for controlling harmful locusts, grasshoppers and katydids. This is especially important in sensitive areas where the use of chemical insecticides is legally prohibited (water protection zones, specially protected natural areas, agricultural land for growing organic products.
:处理蝗虫和蚱蜢等大规模害虫的虫害防治服务机构被迫使用各种来源的杀虫剂,以防止作物受损。在大多数情况下,主要使用化学杀虫剂。这种控制害虫数量的方法有许多负面后果。由于使用广谱杀虫剂进行化学处理,不仅可以杀死害虫,还可以杀死非目标生物,主要是食虫和传粉者。化学控制方法的替代方法之一是使用基于昆虫致病病毒、细菌和真菌的生物农药。在哈萨克斯坦东南部的大豆种植中,研究了以真菌lecanthomyces lecanii为基础的生物农药Entolek K Planteco®对不同种类的蝗虫、蚱蜢和螽斯的影响。处理后第3天和第7天,蝗虫和蚱蜢的死亡率分别为70%和98%,同一天的蝈蝈死亡率分别为50%和70%。因此,“Entolek K Planteco®”被证明是有效的控制有害的蝗虫,蚱蜢和蝈蝈。在法律禁止使用化学杀虫剂的敏感地区(水保护区、特别保护的自然区域、种植有机产品的农业用地),这一点尤为重要。
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引用次数: 2
Water Saving with Combined Irrigation Methods 联合灌溉节水
Q3 Agricultural and Biological Sciences Pub Date : 2022-04-01 DOI: 10.3844/ojbsci.2022.415.424
A. Kalashnikov, N. Balgabaev, V. Zharkov, Ye. V. Angold, P. Kalashnikov
: In the conditions of arid zones of irrigated agriculture, during the cultivation of horticultural and other crops, high air temperatures (over 25-30 о С) and low relative humidity (less than 20-30%) are observed during the vegetation period of plants. In these circumstances, the combined irrigation method using drip irrigation and sprinkling allows optimizing the use of water during drip irrigation at air temperatures below 25-30 о С and improving the microclimate through sprinkling at air temperatures above 25-30 о С. The study aims to determine the effect of the combined irrigation method on the microclimate, water regime of plants, and water productivity compared to the drip irrigation system. The key research method employed is a field experiment conducted on a specially allocated plot of land to establish differences between the two methods of irrigation. The field experiment presupposes an exploratory study and quantifies the effect of the new technology of irrigation of apple trees to objectively justify the implementation of this scientific development in agricultural production. The combined method of irrigation provides a decrease in air temperature by 1.5-2.1°C and increases air humidity by 10-17% in the surface air layer, improving the water regime of plants and increasing the apple yield by 10.7-17.9% against drip irrigation, while reducing water consumption per production unit by 4.0-10.1%. The method of combined irrigation is thus recommended for use in the irrigated agriculture.
:在灌溉农业干旱区的条件下,在园艺和其他作物的种植过程中,在植物的植被期,会观察到高气温(超过25-30℃)和低相对湿度(低于20-30%)。在这种情况下,使用滴灌和洒水的联合灌溉方法可以在25-30°C以下的气温下优化滴灌期间的用水,并通过在25-30ºC以上的气温下洒水来改善小气候。该研究旨在确定与滴灌系统相比,联合灌溉方法对小气候、植物水情和水生产力的影响。所采用的关键研究方法是在一块专门分配的土地上进行田间试验,以确定两种灌溉方法之间的差异。田间试验以探索性研究为前提,量化了苹果树灌溉新技术的效果,以客观地证明在农业生产中实施这一科学发展。联合灌溉使地表空气层的气温降低1.5-2.1°C,空气湿度增加10-17%,改善了植物的水分状况,使苹果产量比滴灌提高10.7-17.9%,同时将单位生产用水量降低4.0-10.1%。因此,建议在灌溉农业中使用联合灌溉方法。
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引用次数: 2
Poly-Species Phytocenoses for Ecosystem Restoration of Degraded Soil Covers 用于退化土壤覆盖生态系统恢复的多物种植物群落
Q3 Agricultural and Biological Sciences Pub Date : 2022-03-01 DOI: 10.3844/ojbsci.2022.268.278
A. Nugmanov, S. Mamikhin, K. Valiev, A. Bugubaeva, Asel S. Tokusheva, S. Tulkubaeva, A. Bulaev
: Agroecological studies are based on the concept that, by understanding ecological processes, it is possible to manage agroecosystems for more sustainable production of more biomass and, thus, protection of natural resources. Many studies in the field of agroecology show that some farming methods can improve soil quality and restore agroecosystems by increasing plant biodiversity. Functional characteristics of plants for the assessment of ecosystem processes and services can be used for the sustainable management of agroecosystems. The paper discusses ways to restore pastures with low biological productivity in the arid steppe of Northern Kazakhstan (Kostanay region). Grass-and-legume mixtures were selected, according to the soil and climatic conditions of this region, to increase the productivity of pastures. The authors also describe the meteorological conditions in the region in question for 2021. The data of agrochemical analysis of the soil on which the grass mixture was grown differed slightly from the soils of other sites and often even was inferior to them in some indicators that affected plant productivity, namely, the humus, phosphorus, and potassium content. In the conditions of the experiment, the highest productivity was shown by the grass-and-legume mixture consisting of crested wheatgrass- alfalfa -awn less brome (27.8 c/ha of green mass and 12.6 c/ha of dry matter). Thus, according to the information obtained during the study, it can be concluded that to restore the productivity of pastures in Northern Kazakhstan, it is necessary to select the grass-and-legume mixtures that have the highest productivity and nutritional value and are adapted to the soil and climatic conditions of this region. The algorithm of the predictive simulation model of the dynamics of the graminoid community is also presented in a general form. Problems related to the remediation of soil covers are discussed.
:农业生态学研究基于这样一个概念,即通过了解生态过程,可以管理农业生态系统,以更可持续地生产更多的生物量,从而保护自然资源。农业生态学领域的许多研究表明,一些耕作方法可以通过增加植物生物多样性来改善土壤质量和恢复农业生态系统。用于评估生态系统过程和服务的植物功能特征可用于农业生态系统的可持续管理。本文讨论了在哈萨克斯坦北部干旱草原(科斯塔奈地区)恢复低生物生产力牧场的方法。根据该地区的土壤和气候条件,选择了草和豆类混合物,以提高牧场的生产力。作者还描述了该地区2021年的气象条件。种植混合草的土壤的农业化学分析数据与其他地点的土壤略有不同,甚至在影响植物生产力的一些指标上,即腐殖质、磷和钾含量,往往不如其他地点。在实验条件下,由羽冠麦草-苜蓿-无芒雀麦组成的草和豆类混合物(27.8 c/ha的绿色物质和12.6 c/ha的干物质)显示出最高的生产力。因此,根据研究期间获得的信息,可以得出结论,为了恢复哈萨克斯坦北部牧场的生产力,有必要选择生产力和营养价值最高、适合该地区土壤和气候条件的草和豆类混合物。还以一般形式提出了拟禾本科群落动力学预测模拟模型的算法,并讨论了与土壤覆盖修复有关的问题。
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引用次数: 3
Effect of Semen on the Embryo Productivity of Donor Cows and the Development of Transplant Calves 精液对供牛胚胎产率及移植犊牛发育的影响
Q3 Agricultural and Biological Sciences Pub Date : 2022-03-01 DOI: 10.3844/ojbsci.2022.356.362
B. Ateikhan, Toktar Karibaevich Bexeitov, T. Seiteuov, K. K. Akhazhanov, Maxim Viktorovich Sirovatsky, Sergey Valeryevich Beketov
: In animal husbandry, more heifers are required for the expanded reproduction of the herd or milk, and more bulls are needed to obtain meat. One of the effective ways to artificially regulate the secondary (at birth) sex ratio is the technology of sexed semen. In this connection, the purpose of this study was to study the effect of sexed and native semen on the embryonic productivity of donor cows, the survival rate of embryos in recipient cows, and the development of transplant calves. The studies were carried out on Simmental cows in the period from 2017 to 2019 in the Galitskoe LLP and Pobeda LLP in the Pavlodar region (Republic of Kazakhstan). It was found that 80 (78.4%) of 102 embryos were suitable for transplantation in cows fertilized with native sperm and 31 of 51 embryos (60.8%) in cows inseminated with sexed sperm. At the same time, embryos at the stage of compact morula and early blastocyst prevailed in both groups of cows. Later, at the time of embryo transplantation to recipient cows, the survival rate was 59.5% for two-sex and 58.3% for single-sex transplants. During the analysis of postnatal development of different-sex transplant calves, it was found that although they were characterized by different growth dynamics depending on sex, they generally exceeded the calves of the control group in this parameter. The detected differences in superovulation and embryo productivity of cows of the Simmental breed show that the individual characteristics of donor cows are decisive regardless of which sperm (native or sexed) was used for artificial insemination. At the same time, in all cases, donor cows fertilized with native semen were distinguished by the best embryo productivity, although the differences with cows inseminated with sexed sperm were not significant .
:在畜牧业中,需要更多的小母牛来扩大牛群或乳汁的繁殖,也需要更多的公牛来获得肉。人工调节次级(出生时)性别比的有效方法之一是性精液技术。在这方面,本研究的目的是研究性精液和天然精液对供体奶牛胚胎生产力、受体奶牛胚胎存活率和移植小牛发育的影响。2017年至2019年期间,在巴甫洛达尔地区(哈萨克斯坦共和国)的Galitskoe LLP和Pobeda LLP对西门塔尔奶牛进行了研究。结果发现,102个胚胎中有80个(78.4%)适合移植于用天然精子受精的奶牛,51个胚胎中的31个(60.8%)适合移植给用性精子受精的母牛。同时,两组奶牛的胚胎均处于致密桑椹胚和早期胚泡阶段。后来,在胚胎移植到受体奶牛时,两性的存活率为59.5%,单性移植的存活率是58.3%。在对不同性别移植小牛的产后发育进行分析时发现,尽管它们的生长动态因性别而异,但在这一参数上,它们通常超过了对照组的小牛。检测到的西门塔尔品种奶牛的超排和胚胎生产率差异表明,无论哪种精子(原生或性别)用于人工授精,供体奶牛的个体特征都是决定性的。同时,在所有情况下,用本地精液受精的供体奶牛都以最佳胚胎生产力来区分,尽管与用性精子受精的奶牛的差异并不显著。
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引用次数: 1
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OnLine Journal of Biological Sciences
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