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Wild Allium longicuspis Regel is a Feral Form of Allium sativum L. in Kazakhstan: A Comparative Molecular Genetic Analysis 野生长叶葱属(Allium longicuspis Regel)是哈萨克斯坦Allium sativum L.的一种野生形态:比较分子遗传分析
Q3 Agricultural and Biological Sciences Pub Date : 2023-01-01 DOI: 10.3844/ojbsci.2023.33.43
A. Tokenova, G. Sitpayeva, N. Gemejiyeva, S. Suleimenova, N. Friesen, D. Batayeva
: The genus Allium L comprises over 1000 species with important nutritional, medicinal and horticultural applications. The natural flora of Kazakhstan is rich in Allium species, including the wild long-pointed onion, A. longicuspis and cultivated A. sativum garlic varieties. It has been hypothesized that A. longicupis is ancestral to A. sativum . We investigated genetic polymorphism in samples of A. longicupis and the A. sativum cultivars “Niki” and “Merey” using start codon targeted polymorphism analysis. Nuclear ribosomal DNA fragments were purified, amplified and tested with 25 primers, finally selecting 12 primers that identified polymorphisms. DNA samples were sequenced, electrophoresed and cluster analysis performed by the construction of dendrograms and principal component analysis. The karyotypes of the different samples were also compared. The results showed that the samples of A. longicupis from natural populations did not differ significantly from those of the cultivated garlic varieties. The results indicate that A. longicupis can be considered a feral, rather than an ancestral, form of A. sativum . These findings have implications both for the breeding and cultivation of garlic varieties and for the taxonomy of the Allium genus.
:葱属植物有1000多种,具有重要的营养、药用和园艺应用。哈萨克斯坦的自然植物区系中有丰富的葱属植物,包括野生长尖洋葱、A.longicuspis和栽培的A.sativum大蒜品种。据推测,A.longicupis是A.sativum的祖先。我们采用起始密码子靶向多态性分析方法,研究了A.longicupis和A.sativum品种“Niki”和“Merey”的遗传多态性。用25个引物对核核糖体DNA片段进行纯化、扩增和检测,最终选择12个引物进行多态性鉴定。通过构建树状图和主成分分析对DNA样本进行测序、电泳和聚类分析。并对不同样品的核型进行了比较。结果表明,自然种群中的长皮A.longicupis样品与栽培大蒜品种的样品没有显著差异。结果表明,A.longicupis可以被认为是A.sativum的野生形式,而不是祖先形式。这些发现对大蒜品种的选育和栽培以及葱属的分类学都有意义。
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引用次数: 2
Features of Growth and Development in Heifers of Holstein and Black-and-White Breeds 荷斯坦和黑白种小母牛生长发育特点
Q3 Agricultural and Biological Sciences Pub Date : 2022-04-01 DOI: 10.3844/ojbsci.2022.529.538
E. Babich, B. Aryngaziev, L. Ovchinnikova, A. Ovchinnikov
: The study of the characteristics of the growth and development of farm animals is of interest in breeding, as it will help to improve animal breeds, as well as serve for the organization of fast and economically rational raising of productive livestock. The research aims to study the characteristics of growth and development and indicators of reproduction of the Holstein and Black-and-White breeds. For research, three groups of 10-day-old heifers were formed. Body weight was considered by individual weighing at birth, at 3, 6, 9, 12, 15 and 18 months. The reproductive indices of heifers were studied according to the data of the log of inseminations and calving of cows: The age at the first insemination, the age at fertilization and the number of inseminations per 1 fruitful insemination were considered. The conducted studies led to a conclusion that the Holstein heifers had an advantage in live weight over the Black-and-White heifers at the age of 6 months by 19.5 kg and 15.5 kg (P≤0.001), at 12 months of age by 18.4 kg (P≤0.01) and 19.2 kg, at 18 months by 28.0 kg and 25.3 kg (P≤0.01), respectively and exceeded them in growth intensity during the entire study period. The Holstein animals matured faster and were inseminated 43.2-45.8 days earlier than the Black-and-White animals that were inseminated at 511 days. Profits from raising the Holstein heifers were higher by $48.79-54.79.
研究农场动物生长发育的特点对育种有重要意义,有助于改良动物品种,为组织快速、经济合理地饲养生产性家畜服务。本研究旨在研究荷斯坦犬和黑白犬的生长发育特点和繁殖指标。为了进行研究,研究人员组成了三组10天大的小母牛。体重以出生时、3、6、9、12、15和18个月时的个体体重计算。根据奶牛的授精对数和产犊数据,研究了母牛的生殖指标:考虑首次授精年龄、受精年龄和每一次有效授精的授精次数。结果表明:荷斯坦小母牛6月龄时的活重分别比黑白小母牛高19.5 kg和15.5 kg (P≤0.001),12月龄时的活重分别比黑白小母牛高18.4 kg (P≤0.01)和19.2 kg, 18月龄时的活重分别比黑白小母牛高28.0 kg和25.3 kg (P≤0.01),且在整个研究期内的生长强度均超过黑白小母牛。荷斯坦鼠成熟速度快,比黑白鼠早43.2 ~ 45.8 d (511 d)授精。饲养荷斯坦小母牛的利润增加了48.79-54.79美元。
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引用次数: 1
Current Growth Conditions of Populus Diversifolia Schrenk and Populus Pruinosa Schrenk in the Syr-Darya Valley Syr-Darya流域中国白杨和中国白杨生长现状
Q3 Agricultural and Biological Sciences Pub Date : 2022-04-01 DOI: 10.3844/ojbsci.2022.425.438
P. Vesselova, K. Makhmudova, G. Kudabayeva, B. Osmonali, V. Mikhalev
: In the context of urbanization of territories and the intensification of agriculture, the biological diversity of animals and plants in their habitats is degrading. The purpose of the study is to determine the edaphic factors affecting the growth of Populus diversifolia Schrenk and Populus pruinosa Schrenk, as well as anthropogenic factors affecting their conservation in the Syr-Darya valley (Kazakhstan). For identifying the range of edaphic growing conditions, the analysis of soil samples gathered in different phytocenoses with the dominance or participation of Turanga was carried out. The following results were presented: Physical and chemical analyses of soil samples for the content of humus, mobile forms of easily hydrolyzable nitrogen, phosphorus, and potassium; pH aqueous solution; type, and degree of salinization. Among the 6 sections studied, 4 belong to alluvial-meadow tugai salt marsh soils, 1-to meadow salt marshes, and 1-to alluvial-meadow strongly salt marsh soils. Populus diversifolia has been found to prefer mild (sandy) and fewer saline conditions than Populus pruinosa . Among the negative anthropogenic impacts on the Turanga groves were: Regulation of the runoff of the Syr-Darya, felling of trees, fires, and grazing. The identified cenoflora was represented by 103 species of higher vascular plants. Among them, Chenopodiaceae (18), Asteraceae (18), and Brassicaceae (12) were leading. The indicative role of Turanga was based on its connection to the places of groundwater occurrence.
:在领土城市化和农业集约化的背景下,动植物栖息地的生物多样性正在退化。本研究的目的是确定影响Syr Darya山谷(哈萨克斯坦)胡杨和普鲁诺萨胡杨生长的土壤因素,以及影响其保护的人为因素。为了确定土壤生长条件的范围,对在图兰加占主导地位或参与的不同植物群落中采集的土壤样本进行了分析。结果如下:对土壤样品的腐殖质含量、易水解氮、磷和钾的流动形式进行了物理和化学分析;pH水溶液;盐碱化的类型和程度。在研究的6个剖面中,4个剖面属于冲积草甸土盖盐沼土,1个剖面属于草甸盐沼,1个断面属于冲积草甸强盐沼土。已经发现,与普鲁诺萨杨相比,胡杨更喜欢温和(沙质)和更少的盐碱条件。对图兰加树林的负面人为影响包括:锡尔河径流的调节、树木砍伐、火灾和放牧。已鉴定的cenoflora由103种高等维管植物代表。其中,藜科(18个)、菊科(18)和十字花科(12个)居首位。图兰加的指示作用是基于其与地下水赋存地的联系。
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引用次数: 4
Integrated Protection of Tomato Crops against Tuta absoluta in Open Ground Conditions in the South-East Part of Kazakhstan 哈萨克斯坦东南部露天番茄作物对赤霉病的综合防治
Q3 Agricultural and Biological Sciences Pub Date : 2022-04-01 DOI: 10.3844/ojbsci.2022.539.548
A. Adilkhankyzy, K. Alpysbayeva, B. Nurmanov, B. Z. Naimanova, N. Bashkarayev, A. Kenzhegaliev, A. Uspanov
: There is a need to develop strategies for the protection of tomato crops that would provide high-quality produce safe for the consumer without environmental pollution. The objective of the study is to deploy an integrated system for the protection of open-field tomato crops from Tuta absoluta using biological agents ( Bracon hebetor Say., Trichogramma, biopreparations, pheromone traps) and devices in the conditions of southeast Kazakhstan to obtain environmentally safe produce. Experiments on the protection of tomato crops from pests are conducted by applying integrated protection measures with the use of entomophages, biopesticides, pheromone traps, and low-hazard pesticides on the tomato field of the Musa private farm. Per the results of the experiments on protecting open-field tomato plants from Tuta absoluta, the effectiveness of the employed instruments reaches up to 90%. The obtained results can be used to protect tomato plants accounting for pest density and the phase of crop development. Thus, based on the results obtained, it can be concluded that the primary elements of the integrated system for the protection of tomato crops against Tuta absoluta should include the application of biological preparations, entomophages and, if necessary, low-hazard insecticides, as well as the use of pheromone traps to detect the appearance of the moths and capture them en masse.
:有必要制定保护番茄作物的战略,在不污染环境的情况下为消费者提供安全的高质量农产品。该研究的目的是在哈萨克斯坦东南部的条件下,使用生物制剂(Bracon hebetor Say.、赤眼蜂、生物补偿、信息素陷阱)和设备,部署一个保护露地番茄作物免受绝对图塔侵害的综合系统,以获得环境安全的产品。在穆萨私人农场的番茄地上,通过使用昆虫噬菌体、生物杀虫剂、信息素诱捕器和低危害农药的综合保护措施,进行了番茄作物免受害虫侵害的实验。根据保护露地番茄免受绝对图塔侵害的实验结果,所用仪器的有效性高达90%。所获得的结果可用于保护番茄植物,考虑害虫密度和作物发育阶段。因此,根据所获得的结果,可以得出结论,保护番茄作物免受绝对毒蛾侵害的综合系统的主要要素应包括应用生物制剂、昆虫噬菌体,必要时还应使用低危害杀虫剂,以及使用信息素陷阱来检测蛾类的出现并将其集体捕获。
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引用次数: 4
Participation of Dermacentor Reticulatus Imago in the Reservation of Bovine Leukemia Virus 斑节皮蛛参与牛白血病病毒的保存
Q3 Agricultural and Biological Sciences Pub Date : 2022-04-01 DOI: 10.3844/ojbsci.2022.456.462
Y. Glazunov, Yana Kabitskaya, L. Glazunova, I. Donnik, E. Boyko, Y. Vinogradova
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引用次数: 0
Retraction Notice: Review of Prodigiosin, Pigmentation in Serratia marcescens 撤回通知:对粘质沙雷氏菌中色素沉着的Prodigiosin的审查
Q3 Agricultural and Biological Sciences Pub Date : 2022-04-01 DOI: 10.3844/ojbsci.2022.549
Notice
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引用次数: 0
Biological Study Scabiosa ochroleuca Seeds Germination and Development of Methods for a Long-Term Storage 赤藓种子发芽及长期贮藏方法的研究
Q3 Agricultural and Biological Sciences Pub Date : 2022-04-01 DOI: 10.3844/ojbsci.2022.512.519
S. Tyrzhanova, Margarita Y Ishmuratova, S. N. Atikeeva, A. Kydyrmoldina, Meruyert Malikovna Malik
: The organization of a storage system for seeds of medicinal plants is an important task of introduction. Our study aimed to identify biological features during the germination of Scabiosa ochroleuca seeds and optimize the conditions for their storage and germination activation. The paper presents the results of assessing the viability of seeds of S. ochroleuca depending on the origin, size, storage time, the use of pre-sowing treatment with epin, potassium humate, the use of barbotage and stratification; the conditions of cryopreservation in liquid nitrogen have been worked out. The results showed that germination capacity and energy depended on origin and size. The best indicators were noted for large and completed seeds collected in the Buyratau Mountains. During storage, a gradual decrease in germination was noticed; the loss of viability was noted in the 4 th year of storage under normal conditions. Stratification can be used for 3 months to activate germination. To organize a long-term storage system, we recommend storing S. ochroleuca seeds in liquid nitrogen in plastic containers, using glycerol cryoprotectant at a concentration of 40% .
:组织药用植物种子的储存系统是引进的一项重要任务。我们的研究旨在确定赤藓种子发芽过程中的生物学特征,并优化其储存和发芽活化的条件。本文介绍了根据来源、大小、储存时间、使用epin、腐殖酸钾预播处理、使用倒伏和分层来评估石首乌种子活力的结果;确定了液氮冷冻保存的条件。结果表明,种子的发芽能力和发芽能量取决于种子的来源和大小。最好的指标是在Buyratau山脉采集的大型和完整的种子。在贮藏过程中,发芽率逐渐下降;在正常条件下储存的第4年,发现活力丧失。分层可以使用3个月来激活发芽。为了组织长期储存系统,我们建议使用浓度为40%的甘油冷冻保护剂,在塑料容器中的液氮中储存苏丹红种子。
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引用次数: 0
Beneficial Effects of Soybean and Cassava in Local Wisdom Feed Diets on Growth Performance with Nutritional and Economic Analysis of Snakehead Fish (Channa Striata) 大豆和木薯在地方智慧饲料日粮中对斑头鱼生长性能的有益作用及营养经济分析
Q3 Agricultural and Biological Sciences Pub Date : 2022-04-01 DOI: 10.3844/ojbsci.2022.463.468
D. Boonkusol, W. Tongbai
: Substituting plant protein for fish meals is an alternate protein source in the diet of reared fish. By replacing fish meal with Soybean Meal (SM) or Cassava Meal (CM), this study determined the effects of diets influenced by local wisdom containing plant protein meal, SM, CM, or a commercial formula (FM TM ) on the survival and growth rates of snakehead fish ( Channa striata ) with nutritional and economic analysis. After acclimatization, snakehead fingerlings (10 fish/tank) were randomly allocated in the nine cement tanks. Three tanks each for Soybean Meal (SM) diet; Cassava Meal (CM) diet; and commercial diet (FM TM ). Fish weights were determined every four weeks for the 12 weeks. At the end of each experiment, the Survival Rate (SR), growth performance values, and cost reduction were calculated. After 12 weeks, there were no significant differences in SR among treatment groups. The growth performance of fish in SM dietary treatment and FM TM dietary treatments had no significant difference. Although, the specific growth rate of fish fed CM diet was slightly less than SM and FM TM diet .The soybean meal or cassava was substituted for a fish meal resulting in 11.6 and 7.9% economic value (cost/100g fish product), respectively compared to the commercial diet. However, the CM diet resulted in the lowest Feed Conversion Rate (FCR) in this experiment. There were no significant differences between FCR and Protein Efficiency Rate (PER). The results revealed the beneficial effect of diets influenced by local wisdom, especially the addition of soybean, in terms of weight gain promotion and feed cost reduction. These findings can be applied to develop low-cost diets for snakehead fish, supporting the flexibility and competitiveness of the snakehead fish farming industry and reducing the number of small fish used for fishmeal.
:用植物蛋白代替鱼粉是饲养鱼类饮食中的另一种蛋白质来源。通过用豆粕(SM)或木薯粉(CM)代替鱼粉,本研究通过营养和经济分析,确定了受含当地智慧的植物蛋白粉、SM、CM或商业配方(FM TM)影响的饮食对黑钱鱼(Channa striata)存活率和生长率的影响。驯化后,在9个水泥罐中随机分配10条鱼/罐的蛇头鱼种。豆粕(SM)日粮各三个罐;木薯粉(CM)饮食;和商业饮食(FM TM)。12周内每四周测定一次鱼的重量。在每个实验结束时,计算存活率(SR)、生长性能值和成本降低。12周后,各治疗组的SR没有显著差异。SM日粮处理和FMTM日粮处理的鱼类生长性能无显著差异。尽管如此,CM日粮中鱼类的比生长率略低于SM和FMTM日粮。与商业饮食相比,用豆粕或木薯代替鱼粉,分别产生11.6%和7.9%的经济价值(成本/100克鱼产品)。然而,在本实验中,CM日粮的饲料转化率(FCR)最低。FCR和蛋白质效率(PER)之间没有显著差异。结果表明,受当地智慧影响的饮食,特别是添加大豆,在促进体重增加和降低饲料成本方面具有有益效果。这些发现可用于开发低成本的乌鱼日粮,支持乌鱼养殖业的灵活性和竞争力,并减少用于鱼粉的小型鱼类数量。
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引用次数: 0
Parasite Fauna and Infection of Sheep with Parasitosis 寄生虫区系与绵羊感染寄生虫病
Q3 Agricultural and Biological Sciences Pub Date : 2022-04-01 DOI: 10.3844/ojbsci.2022.404.414
Assylbek Abdrashitovich Zhanabayev, Birzhan Yeleubaevich Nurgaliev, A. Kereyev, A. Y. Paritova, Altay Y. Ussenbayev, S. Bayantassova, Dinara M. Seitkamzina, G. K. Zhumagaliyeva, Botakoz Yelemessova
: The purpose of our research was to study the parasitic fauna and infection of sheep with parasites in the northern and southern regions of the Republic of Kazakhstan. For this, we conducted a study to determine the species diversity of sheep parasites. The study was set from 2010-2020 years on infected sheep in the northern and southern parts of the Republic of Kazakhstan. The experiments were conducted at the Department of Veterinary Medicine of S. Seifullin KazATU and the KazNIVI in Almaty. The species diversity of parasites of adult sheep was studied. The materials for parasitological laboratory studies were sheep feces taken from 50 sheep. Vital diagnostics of helminthiasis were carried out by scatological studies of 50 sheep using the well-known methods of Fulleborn, Weid, and Boyahchyan. To establish the timing of infection, selective coprolarvoscopic studies of fecal samples taken from pastures were carried out. First, the invasive larvae were isolated from fecal samples by the Berman-Orlov method. Differential diagnosis of trichostrongylides was carried out according to the method of Y.Y. Shumakovich. Among the sheep of the Akmola and Karaganda regions, strongylatoses parasitizing in the gastrointestinal tract are recorded: Nematodyrosis, ostertagiosis, hemonhoz, marshallagiasis, esophagostomosis, and, less often, bunostomosis. The development of eggs and larvae of the invasive stage in pasture conditions is noted at the end of the first decade of May. The first cases of infection of lambs are observed in late May-early June. Thus, based on the three-year results obtained, it can be concluded that the absence of antiparasitic measures leads to infection of almost all livestock within 5-7 months and in subsequent years, reinfection occurs, which ultimately causes a decrease in productivity and increase mortality, especially among young animals up to 1.5 years of age.
:我们研究的目的是研究哈萨克斯坦共和国北部和南部地区的寄生虫动物群和绵羊感染寄生虫的情况。为此,我们进行了一项研究,以确定绵羊寄生虫的物种多样性。这项研究是从2010-2020年开始对哈萨克斯坦共和国北部和南部受感染的绵羊进行的。实验在S.Seifullin KazATU兽医系和阿拉木图的KazNIVI进行。对成年绵羊寄生虫的种类多样性进行了研究。寄生虫学实验室研究的材料是取自50只绵羊的绵羊粪便。采用著名的Fulleborn、Weid和Boyahchyan方法,对50只绵羊的粪便学研究进行了蠕虫病的重要诊断。为了确定感染的时间,对从牧场采集的粪便样本进行了选择性粪镜检查研究。首先,通过Berman-Orlov方法从粪便样本中分离出入侵幼虫。根据Y.Y.Shumakovich的方法对三尖叶菌进行鉴别诊断。在阿克莫拉和卡拉干达地区的绵羊中,记录了寄生在胃肠道的strongylatose:线虫病、骨管病、血竭病、马歇尔病、食道造口术,以及不太常见的拇囊造口术。牧场条件下入侵期的卵子和幼虫在5月的第一个十年结束时发育。羔羊感染的第一例病例发生在5月底至6月初。因此,根据所获得的三年结果,可以得出结论,缺乏抗寄生虫措施会在5-7个月内导致几乎所有牲畜感染,在随后的几年中,再次感染会发生,最终导致生产力下降,死亡率增加,尤其是在1.5岁以下的幼兽中。
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引用次数: 5
Visual-Spatial Attention Abilities Among School Children Exposed to Low Environmental Lead Level 低环境铅含量学童的视觉空间注意能力
Q3 Agricultural and Biological Sciences Pub Date : 2022-04-01 DOI: 10.3844/ojbsci.2022.439.447
Sana Maidoumi, Mariam Ouisselsat, Charif Radouan Ouaziz, Nadia Abbassi, M. Loukid, A. Ahami, A. Pineau, A. Sedki
: Lead (Pb) is an intrusive toxic matter which harms the neurocognitive and behavioral development of children even with doses considered normal. The present paper aims at evaluating the visual-spatial attention function among 142 schoolchildren living in a mining area (cases) in comparison to 177 living in the Chouiter area (control group) in Marrakech. We used the Bell Dam Test (BDT) for the assessment of their visual attentional abilities and unilateral spatial neglect. Interviews with parents were conducted to identify co-variables related to Pb exposure. Agricultural soil, drinking, and groundwater were collected from the surrounding area of the children's school. The determination of Pb content in the environment was performed by ICP-MS. The results demonstrated that cases' scores in the test of visual attentional abilities and in scanning procedures were significantly worse in comparison to the control group (p = 0.05 and p = 0.002, respectively). In cases, soil Pb level was higher (67.14±10.13 g/g) than in controls (41.73±13.6 g/g). Pb levels in groundwater and drinking water were low with a significant difference between the two areas in groundwater. The mean scores of visual attentions were significantly lower in children exposed to environmental Pb contamination compared to those who are not exposed (p = 0.008). These findings suggest that Pb is probably incriminated in the genesis of visual attention deficit.
铅(Pb)是一种侵入性有毒物质,即使在正常剂量下也会损害儿童的神经认知和行为发育。本论文旨在评估生活在马拉喀什矿区的142名学童(案例)与生活在Chouiter地区的177名学童(对照组)的视觉空间注意功能。采用贝尔坝测试(BDT)对视觉注意能力和单侧空间忽视能力进行评价。对家长进行访谈,以确定与铅暴露有关的协变量。农业土壤、饮用水和地下水都是从儿童学校周围地区收集的。采用电感耦合等离子体质谱法测定环境中铅的含量。结果表明,患者在视觉注意能力测试和扫描程序中的得分明显低于对照组(p = 0.05和p = 0.002)。试验组土壤铅水平(67.14±10.13 g/g)高于对照组(41.73±13.6 g/g)。地下水和饮用水中铅含量均较低,两地地下水中铅含量差异显著。环境铅污染儿童的视觉注意平均得分明显低于未暴露儿童(p = 0.008)。这些发现表明铅可能与视觉注意缺陷的发生有关。
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引用次数: 0
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OnLine Journal of Biological Sciences
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