Journal of Contemporary Dental Practice最新文献

Aim: To evaluate the role of erbium lasers (Er:YAG and Er,Cr:YSGG) as adjuncts in access flap periodontal surgery and their potential contribution to personalized, minimally invasive periodontal care.

Background: Periodontal surgery has progressively evolved toward minimally invasive techniques prioritizing tissue preservation, enhanced healing, and reduced morbidity. Erbium lasers represent a significant advancement by offering precise tissue ablation, improved decontamination, and favorable patient experiences compared to conventional approaches.

Review results: Evidence shows that erbium lasers achieve clinical outcomes comparable to or better than traditional flap surgery. Benefits include reduced postoperative discomfort, minimized gingival recession (GR), and enhanced patient satisfaction. While gains in clinical attachment level are modest yet statistically significant in some studies, patient-reported outcomes (PROs) consistently favor erbium-assisted interventions.

Conclusion: Erbium lasers enhance access flap surgery by supporting precision, soft tissue preservation, and improved patient-centered outcomes. Current data suggest they are valuable adjuncts, though long-term trials are still needed to establish standardized protocols.

Clinical significance: Integration of erbium lasers into periodontal flap surgery supports personalized and minimally invasive care, aligning with modern principles of precision periodontics and improving both clinical and PROs. How to cite this article: Neophytou C, Neofytou AM, Davidopoulou S, et al. Erbium Laser-assisted Access Flap Periodontal Surgery: Advancing Personalized Periodontal Care through Minimally Invasive Technologies. J Contemp Dent Pract 2025;26(11):1025-1034.

Aim: This study aims to examine the pro-apoptotic and anti-migratory effects of Berberine in KB-1 oral cancer cells and its role in ferroptosis via glutathione peroxidase4/six-transmembrane epithelial antigen of prostate3 (GPX4/STEAP3) signaling pathway.

Materials and methods: KB-1 cells were treated with Berberine, and cell viability was assessed using the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. Morphological changes were analyzed via phase-contrast microscopy, while nuclear fragmentation was examined using acridine orange/ethidium bromide (AO/EtBr) staining. Molecular docking, gene expression analyses were performed to assess ferroptosis markers. Wound healing and migration assays evaluated Berberine's effect on cell migration.

Results: Berberine reduced cell viability (IC50: 22.2 μM) and induced morphological changes, including shrinkage and membrane blebbing. AO/EtBr staining confirmed nuclear fragmentation. Gene expression analysis showed ferroportin upregulation and GPX4, STEAP3, and transferrin receptor 1 (TFR1) downregulation, validated by western blotting. Molecular docking confirmed strong Berberine binding, particularly with GPX4 and STEAP3. Wound healing and migration assays showed significant migration suppression. All experimental data were statistically evaluated using the Student's t-test and one-way ANOVA.

Conclusion: Berberine induces ferroptosis and inhibits KB-1 oral cancer cell proliferation and migration by modulating the ferroptosis signaling pathway, suggesting its potential as a novel therapeutic agent.

Clinical significance: By promoting iron-dependent cell death and suppressing migratory capacity, Berberine may overcome resistance to conventional therapies and reduce metastatic progression. Its natural origin and prior safety profile support drug repurposing opportunities, though challenges such as poor bioavailability and systemic toxicity must be addressed. These findings provide a foundation for biomarker-driven preclinical and early clinical evaluations. How to cite this article: Alassiri S. Berberine Induces Ferroptosis and Impairs Migration via Glutathione Peroxidase4/Six-transmembrane Epithelial Antigen of Prostate3 Signaling Pathway in Oral Cancer Cells. J Contemp Dent Pract 2025;26(11):1050-1059.

Aim: Despite the widespread availability of online information about dental veneers, there is a lack of data on the quality and readability of these resources. The aim of this study was to assess the quality and readability of patient-oriented online information on dental veneers.

Materials and methods: This study conducted a thorough web search utilizing Google, Yahoo, and Bing search engines to identify English-language websites offering information on dental veneers. The quality of the websites was assessed using DISCERN, Journal of the American Medical Association (JAMA) benchmarks, and Health on the Net Code (HONcode) tools. The readability of the websites was evaluated using the Flesch-Kincaid Grade Level (FKGL), Simplified Measure of Gobbledygook (SMOG), and Flesch Reading Ease (FRE) metrics.

Results: Out of a total of 195 websites included in the study, only 8 websites obtained a high overall DISCERN score, representing 2.4% of dental clinic (DC) and 14.8% of nonprofit organization (NPO) websites. The median [interquartile range (IQR)] of the overall score of DISCERN was significantly higher for NPO websites [55.5 (13)] compared to DC websites [42 (13)] and commercial entity (CE) websites [36.25 (5.5); p < 0.001]. Up to 15, 63, and 70% of NPO, DC, and CE websites, respectively, did not report any of the four JAMA criteria. Only seven websites (all NPOs) showed an active Health on the Net (HONs) Code certificate. All readability indicators confirmed easier texts on the NPO websites.

Conclusions: The quality of the English web-based health information on dental veneers seems suboptimal. Nonprofit organization websites offer higher quality, more reliable, and readable content compared to DC and CE websites.

Clinical significance: Online dental veneer information is generally of poor quality, with NPO websites offering the most reliable and readable content. Clinicians should direct patients to trustworthy NPO resources for accurate information. How to cite this article: Alqutaibi AY, Alharbi AT, Alassaf MS, et al. Assessment of Quality and Readability of Online Patient-centered Information on Dental Veneers: An Infodemiological Study. J Contemp Dent Pract 2025;26(11):1060-1066.

Aim: This study aimed to evaluate in vitro the effect of a natural mouthrinse and alcohol-free 0.12% chlorhexidine on force degradation of orthodontic elastomeric chains from four commercial brands: Ormco Generation II Power Chain, TP E-Chain, American Orthodontics, and Morelli.

Materials and methods: An experimental design was employed using 108 samples, divided into three groups: Control, chlorhexidine, and natural rinse. Force measurements were taken at baseline, after 24 hours, and after 30 days using a high-precision digital dynamometer. Data were analyzed using a three-way ANOVA (Brand × Rinse × Time) and a multiple linear regression model, with significance set at p < 0.05.

Results: The findings revealed a biphasic degradation pattern, characterized by a sharp decline in force during the first 24 hours, followed by a more gradual reduction over the next 30 days. Among the brands, TP demonstrated the lowest force degradation compared to Ormco (approximately -9.79 percentage points), while American Orthodontics and Morelli showed significantly higher losses (approximately +17.31 and +16.17 percentage points, respectively). Exposure to chlorhexidine increased degradation (approximately +4.66 percentage points), whereas the natural mouth rinse did not have a significant overall effect. Additionally, time was a crucial factor: After 30 days, degradation increased by approximately +2.41 percentage points compared to the 24-hour mark.

Conclusion: Degradation of elastomeric chains is influenced by the brand, type of mouth rinse, and duration of exposure. The natural mouthrinse was a safe option to prevent accelerated degradation, thus helping to maintain effective therapeutic forces in orthodontic treatments. These findings emphasize the importance of selecting the appropriate chain brand and mouthrinse, as well as considering the frequency of replacement, to optimize outcomes in orthodontic treatment.

Clinical significance: The strength loss of elastomeric chains depends on the brand, the type of mouthwash used, and the duration of exposure; orthodontists should consider these factors when planning treatments. How to cite this article: Sarzosa-Montenegro JL, Arriola-Guillén LE, Rodríguez-Cárdenas YA. Effects of Natural Mouthwash on the Force Degradation of Orthodontic Elastomeric Chains: An In Vitro Experimental Study. J Contemp Dent Pract 2025;26(11):1035-1040.

Aim: This study aims to evaluate the effectiveness of hyaluronic acid (HA) and mint-flavored toothpaste in the recovery from persistent sensory loss.

Materials and methods: A pilot study of 16 adult patients suffering from anosmia or severe hypoanosmia in Baghdad was selected purposively from a larger sample of 135, examined using the Modified Connecticut Chemosensory Clinical Research Center ("modified CCCRC") test between October 2023 and January 2024 and then re-examined 3 weeks later after twice-daily use of the toothpaste, using the same repeatable, objective method, the modified CCCRC test.

Results: The majority of participants were within the age-group of >35 years at 75%, and 56% were males. There was a significant difference between the median composite scores of thresholds and identification of the smell beside the taste score before and after using the toothpaste (p < 0.001, 0.023), respectively.

Conclusion: Smell and taste scores increase after weeks of daily twice using the hyaluronic toothpaste.

Clinical significance: The combination of HA and mint odor in the same product was effective in promoting the recovery of the sense of smell and improving the quality of life of the study participants. How to cite this article: Abdulrida FM, Hadartie SF, Hobballah AI. The Effect of Hyaluronic acid Toothpaste in Smell and Taste Recovery: A Pilot Study. J Contemp Dent Pract 2025;26(11):1103-1108.

Aim: The study aims to evaluate the effect of fluoridated and non-fluoridated mouthwash on the rate of tooth movement and surface topography changes of nickel-titanium (NiTi) and multistranded stainless steel arch wires.

Materials and methods: Twenty patients aged 18-30 years with moderate mandibular anterior crowding [Little's Irregularity Index (LII): 4-6 mm] were selected and randomly divided into two groups (n = 10) and were assigned by archwire type: Group I (NiTi) and group II (multistranded SS). Each group was subdivided into fluoridated (Colgate MaxFresh Mouthwash; groups IA and IIA) and non-fluoridated (Listerine; groups IB and IIB) mouthwash subgroups (n = 5). The rate of tooth movement was assessed monthly for 3 months using LII, and the surface topography of the retrieved wires was evaluated using scanning electron microscopy (SEM). Data were recorded and statistically analyzed.

Results: Both group I and group II demonstrated a reduction in LII over the 3 months. The non-fluoridated subgroup of group I had the greatest reduction (from 5.58 ± 0.12 mm at T0 to 0.76 ± 0.03 mm at T3) compared to the fluoridated subgroup (from 4.80 ± 0.11 to 0.50 ± 0.07 mm). For group II, the non-fluoridated subgroup reduced from 5.26 ± 0.12 mm to 0.73 ± 0.04 mm, and the fluoridated subgroup reduced from 4.27 ± 0.15 mm to 0.43 ± 0.07 mm. Intragroup comparisons were statistically significant over time (p < 0.001; Friedman test), and intergroup differences at each time point also proved statistically significant (p < 0.05; Kruskal-Wallis test). The SEM showed more significant surface damage (pitting, corrosion, and inclusions) in wires exposed to the fluoridated mouthwash group than the non-fluoridated rinse.

Conclusion: Non-fluoridated mouth rinses seem to aid in orthodontic tooth movement rates, especially with NiTi arch wire, while fluoridated mouth rinses can inhibit arch wire efficacy.

Clinical significance: This study addresses the lack of in vivo evidence regarding the impact of frequently used mouth rinses on orthodontic treatment efficiency. These findings will assist in selecting adjunctive mouth rinses to maintain the integrity of the arch wire and improve the rate of movement during the alignment phase. How to cite this article: Gadde H, Kumar S, Bhattu BK, et al. Effect of Fluoridated and Non-fluoridated Mouthwash on Tooth Movement and Surface Topography Changes of Arch Wires: An In Vivo Study. J Contemp Dent Pract 2025;26(11):1079-1086.

Aim: The current study aimed to evaluate the penetration depth and sealing ability of three different endodontic sealers.

Materials and methods: A total of 60 extracted human mandibular premolars for orthodontic reasons with a single canal were divided randomly into three groups of 20 samples-Group I: Tubli-Seal [zinc oxide-eugenol (ZOE)-based sealer], group II: Endosequence [bioceramic (BC) sealer], group III: AH Plus (epoxy resin-based sealer). At the coronal, middle, and apical thirds, three transversal segments of 1 mm thickness were created using a low-speed precision IsoMet cutter running at 200 rpm while being continuously cooled by water. Following a gold-palladium sputtered coating, these slices were seen at ×1000 magnification using a scanning electron microscope (SEM). The gaps in between the sealer and the canal wall were measured by analyzing images. Data was recorded and statistically analyzed.

Results: The maximum sealing ability was found in Endosequence BC sealer i.e., 3.96 ± 0.16 followed by AH Plus sealer i.e., 2.32 ± 0.08 and Tubli-Seal sealer i.e., 1.04 ± 0.10. On intergroup comparison at coronal third; a statistically significant difference was obtained between Tubli-Seal sealer vs Endosequence BC sealer and Endosequence BC sealer vs AH Plus sealer. At middle and apical third, significant difference was obtained between Tubli-Seal sealer vs Endosequence BC sealer, Tubli-Seal sealer vs AH Plus sealer and Endosequence BC sealer vs AH Plus sealer (p < 0.001).

Conclusion: On conclusion, according to the current investigation, Endosequence BC sealer had the best sealing ability, followed by AH Plus sealer and Tubli-Seal sealer.

Clinical significance: The effectiveness of endodontic therapy depends on proper root canal closure. There are many endodontic sealers available; finding easy-to-use and effective items is crucial to providing quality patient care. When a root canal system is fully sealed, oral pathogens cannot colonize and re-infect the root and periapical tissues. How to cite this article: Mohan D, Lahiri B, Sreenivasalu PKP, et al. Assessment of the Sealing Ability and Penetration Depth of Different Endodontic Sealers: A Scanning Electron Microscope Study. J Contemp Dent Pract 2025;26(11):1092-1095.

Aim: To analyze the bibliometric parameters of scientific output on the use of electronic cigarettes (ECs) and their implications for oral health.

Materials and methods: A bibliometric study analyzing original articles indexed in Scopus on ECs and oral health published between 2015 and 2024. A search strategy based on MeSH terms was implemented. The metadata were exported and analyzed using VOSviewer for co-authorship networks and with SciVal for productivity, impact, and collaboration indicators.

Results: A total of 204 original articles were included. Vohra Fahim was the most productive with 7 (3.4%) articles, while Rahman Irfan Ur (88.8) stood out for the highest average number of citations. The Journal of the American Dental Association had 10 (4.9%) publications, while the Journal of Periodontology showed the greatest impact (41.6). The United States concentrates scientific activity, presenting a wide network of collaboration. International collaboration, although less frequent (31%), had the highest average number of citations per publication (48.3). King Saud University was the most productive with 15 (7.3%) articles.

Conclusion: Scientific research on e-cigarettes and oral health shows the leadership of the United States and the growing participation of Saudi Arabia. International collaboration demonstrates greater visibility and impact.

Clinical significance: E-cigarettes, often perceived as a less harmful option, have a negative impact not only on oral health but also on systemic health. Therefore, it is necessary to strengthen public health strategies to reduce risk factors, support community education, and promote smoke-free environments. How to cite this article: Barja-Ore J, Guillen-Calle BE, Liñan-Bermudez A, et al. Electronic Cigarettes and Oral Health: Bibliometric Mapping of Scientific Evidence. J Contemp Dent Pract 2025;26(11):1109-1114.

Aim: This in vitro study evaluated the microtensile bond strength (µTBS) of two nanohybrid resin composites to caries-affected dentin (CAD) after pretreatment with different hesperidin (HPN) concentrations, before and after thermocycling.

Materials and methods: Eighty extracted human molars were assigned to two groups by composite type (Filtek Z250 XT, Grandio; n = 40 each) and subdivided into four pretreatment protocols (n = 10): control (no treatment), 0.5%, 1%, or 2% HPN. Half of the specimens in each subgroup were subjected to thermocycling. Microtensile bond strength was measured using a universal testing machine. Data were analyzed using two-way and three-way ANOVA followed by post hoc tests (α = 0.05).

Results: Bond strength was significantly affected by HPN concentration and thermocycling. Non-aged Filtek and Grandio showed higher values (up to 39.90 ± 10.67 and 45.87 ± 13.37 MPa, respectively), while aging reduced strength in specific subgroups (Filtek no-treatment: p = 0.016; Grandio 0.5% HPN: p = 0.004; 2% HPN: p = 0.033). Overall, 0.5% HPN maintained better adhesive performance after aging, whereas higher concentrations showed diminished stability. Failure mode analysis supported these trends, with higher bond strength linked to cohesive/mixed failures.

Conclusion: Pretreatment with 0.5% HPN significantly improved bond strength and durability to CAD, while higher concentrations reduced performance, especially after thermocycling.

Clinical significance: Applying 0.5% HPN as a dentin pretreatment may enhance the longevity of composite restorations in cases involving CAD. How to cite this article: Abdel-Salam FD, Sheta N, Eid EG. Effect of Matrix Metalloproteinase Inhibitors on the Bonding Durability of Nanocomposite Resin to Caries-affected Dentin: An In Vitro Study. J Contemp Dent Pract 2025;26(11):1096-1102.

Aim: This study aimed to evaluate the prevalence, patterns, and severity of iatrogenic damage to adjacent teeth during fixed prosthodontic tooth preparation.

Methodology: A total of 126 teeth were assessed following fixed prosthodontic preparation procedures. The adjacent tooth surfaces were examined clinically to determine the level (degree), location, and type of damage on both mesial and distal surfaces. Damage severity was graded as normal, mild, or severe; locations were recorded according to the specific third of the surface involved, and damage type was categorized as abrasion, nick, or both. A Chi-square test was performed to explore differences based on tooth position (maxillary vs mandibular) and operator experience (clinicians, interns, and students).

Results: Normal surfaces were the most frequently recorded finding, accounting for 155 (61.5%) of all assessed surfaces. Mild damage was observed in 77 (30.5%) of cases, while severe damage was less common in 19 (7.5%). The middle third of the proximal surface was the most affected site among damaged teeth. Nicks were the predominant damage type 66 (26.2%), followed by abrasion 21 (8.3%). Maxillary teeth exhibited more severe damage, particularly on mesial surfaces, whereas mandibular teeth more often remain undamaged. A statistically significant difference was observed based on operator experience; students recorded a higher frequency of both mild and severe damage compared to interns and clinicians.

Conclusion: Iatrogenic damage to adjacent teeth during fixed prosthodontic preparation was commonly observed, with mild damage and nicks affecting the middle third of the proximal surface being the most frequent. Severe damage occurred more on the maxillary mesial surfaces.

Clinical significance: Iatrogenic damage was more pronounced on maxillary mesial surfaces and occurred predominantly in preparations performed by less experienced operators. How to cite this article: Alharbi MF, Agwan MAS, Alrethia RA. Prevalence and Patterns of Iatrogenic Damage to Adjacent Teeth during Fixed Prosthodontic Preparation. J Contemp Dent Pract 2025;26(11):1067-1072.