Journal of Contemporary Dental Practice最新文献

Aims: This study investigated the effect of injection of adipose stem cells (ASCs) on the expression of type VII and VIII collagen in Wistar rat's gingiva. Adipose stem cells can modulate the immune system, angiogenesis, wound healing, and extracellular matrix (ECM) remodeling.

Materials and methods: Ten Wistar rats aged three months were divided into two groups: the treatment group and the control group. The Wistar's gingival sulcus between the two incisor teeth was wounded with curettage. In the control group, PBS 1 × 10⁶ was injected, meanwhile, the treatment group was injected with ASCs 1 × 10⁶, then the Wistar rats were terminated on the 14th day and the expression of type VII and VIII collagen was observed, and examined using the immunohistochemical method.

Results: In Wistar rats injected with ASCs, collagen VIII increased more than type VII collagen.

Conclusion: Adipose stem cells can increase the expression of collagen VIII compared to collagen VII.

Clinical significance: Adipose stem cells can influence collagen VII and VIII expression because ASCs release growth factors to restore damaged tissue. Collagen VIII increased more than type VII collagen because type VIII collagen contains integrin receptors, which aid in extracellular protein matrix interactions. Adipose stem cells have multiple signal recognition molecules on the cell membrane, which can be used as potential carriers for drug delivery. So ASCs can be used as an effective and promising method for periodontal treatment. How to cite this article: Hale YA, Sobrina N, Aljunaid MA, et al. Effect of Adipose Stem Cells Injection on Type VII and VIII Collagen Expression of Wistar Rat's Gingiva. J Contemp Dent Pract 2024;25(9):809-813.

Aim: This study aimed to assess if the addition of origanum oil to denture materials could decrease microorganisms counts and biofilm formation without changing their mechanical/surface properties.

Materials and methods: A total of 66 resilient denture liner discs (SoftConfort, Dencril Comércio de Plásticos Ltda, SP, Brazil) were prepared with fixed dimensions of 10 × 3 mm for biofilm assay (n = 36) and 12 × 2 mm for sorption-solubility tests (n = 30) containing three oil concentrations - 0, 2.5 and 5%, thereby n = 12 per each group samples for biofilm assay and n = 10 per each group for sorption-solubility test respectively. While the microcosm biofilms of Streptococcus mutans, Candida species, and total microorganisms formed on denture liner were counted and expressed as colony-forming units per disc surface area, the water sorption (WS) and solubility (SL), was calculated by weighing the samples with an analytical balance at different intervals after storing them in a desiccator and distilled water alternatively. Data was recorded and statistically analyzed.

Results: Surface roughness increased in all groups after biofilm formation (p < 0.001), with the most significant change observed in the 2.5% oil group. The tested oil concentrations did not result in sorption changes, but a 5% oil concentration resulted in higher solubility (p < 0.001). A reduction of total microorganisms and S. mutans was seen after 24 hours for all concentrations (p < 0.05). No significant reduction was found for Candida (C.) albicans after 24 hours, whilst 2.5% oil concentration presented lower counts of C. albicans in comparison to the 5% group after 24 hours (p < 0.05).

Conclusions: Incorporating 2.5% origanum essential oil into resilient denture liners seems to reduce microorganisms count in a complex biofilm model. These results need to be confirmed in future studies.

Clinical significance: The addition of natural products like origanum oil into denture-based materials can help manage biofilm onset and development while offering a simple and effective approach for maintaining denture hygiene. This strategy enhances the antimicrobial properties of denture liners without significantly altering their mechanical and surface characteristics, potentially improving patient outcomes. How to cite this article: Feitosa MÁL, Poletto-Neto V, Maske TT, et al. Surface Modifications and Antifungal Efficacy of Origanum Oil Incorporation in Denture-based Materials: An In Vitro Study. J Contemp Dent Pract 2024;25(9):878-884.

Aim: This study aims to evaluate the impact of platelet-rich fibrin (PRF) and platelet-rich plasma (PRP) on pain, swelling, trismus, soft tissue healing and bone regeneration following mandibular third molar extraction.

Material and methods: A systematic review was conducted from a period of January 2014 to June 2024 using PRISMA guidelines. The search strategy included databases such as Scopus, PubMed, Google Scholar, and Cochrane Central Register of Controlled Trials, using key terms related to "PRF", "PRP", oral surgery, and third molars. PICO criteria followed were - Patient and population (P): Patient with mandibular third molar impacted tooth. Intervention (I): PRF; Comparator or control group (C): PRP; Outcomes (O): Impact on pain, swelling, trismus, soft tissue healing and bone regeneration following mandibular third molar extraction. National Heart, Lung, and Blood Institute (NHLBI) quality assessment tool was also employed. Data was extracted and analyzed.

Results: Six articles met the inclusion criteria. Both materials showed potential in promoting bone and soft tissue regeneration. Out of which 1 was a split-mouth prospective clinical study, 3 were split-mouth comparative study, 2 were unilateral randomized comparative prospective study. While four studies showed a significant improvement in the soft tissue wound healing and increase in bone density in PRF site comparatively, two studies showed no significant difference between PRF and PRP with regard to pain, trismus, swelling and bone formation in the third molar extraction socket region after the placement. Based on NHLBI quality assessment tool, all the studies scored predominantly one, and hence were found good.

Conclusion: Both "PRF" and "PRP" positively influence healing after mandibular third molar extraction. Platelet-rich fibrin offers an advantage due to its ease of preparation and complete autologous nature.

Clinical significance: The impacted third molars created a variety of problems and required their extraction. Because mandibular third molars have denser cortical bone, they are more likely to experience postoperative complications. Successful surgical extraction typically occurs between ages 24 and 30, but outcomes vary based on several factors, including operator experience and patient characteristics. Recent advancements emphasize the role of oxygen and growth factors in wound healing, particularly "PRP" and "PRF". How to cite this article: Pattnayak A, Ramanna PK, Mahabala KY, et al. Impact of Platelet-rich Plasma and Platelet-rich Fibrin in Mandibular Third Molar Extraction: A Systematic Review. J Contemp Dent Pract 2024;25(9):904-910.

Aim: This study intended to comprehend the effects of injectable platelet-rich fibrin (i-PRF) on anchor loss and space closure rates during the retraction phase of orthodontic treatment.

Materials and methods: Twenty-four participants with malocclusion, necessitating extractions and space closure during orthodontic treatment, were enrolled and divided into two groups (n = 12 participants) group A: the experimental group was administered i-PRF on the maxilla/mandible, while group B: the control group did not. Measurements of the rate of space closure, anchor loss, and salivary enzyme activity were done before retraction (T0), after three weeks (T1), after six weeks (T2), and after nine weeks (T3). The Mann-Whitney and the independent Student's t-test were used to compare continuous variables among groups.

Results: Four participants were lost to follow-up in each group with eight participants remaining in the respective groups. The rate of space closure in the maxillary arch was 1.4 ± 1.9 mm at T3 intervals with the baseline value (T0) 10.8 ± 3.01 mm, and the rate of anchor loss was 0.57 mm at T3 intervals for group A. In group B, space closure at T0 was 11.1 ± 2.0 mm and 4.9 ± 1.5 mm at T3 while anchor loss of 0.57 mm at T3 intervals, respectively. In the mandibular arch, the rate of space closure was 2.6 ± 2.0 mm at T3 intervals, with the baseline value (T0) 9.5 ± 2.5 mm, and the rate of anchor loss was 0.325 mm at T3 for group A. In group B, space closure at T0 (baseline) were 10.0 ± 2.7 mm and 4.7 ± 2.3 at T3 mm, and anchor loss was 0.37 mm at T3 intervals, respectively. Space closure rate in the maxilla is significant statistically at intervals T2 and T3 and in the mandible is significant statistically at intervals T2, the anchor loss in both the arch is statistically insignificant.

Conclusion: In both maxillary and mandibular arches, the experimental group showed accelerated tooth movement compared to the control group, although statistical significance was not achieved in the mandible. There were no apparent differences in anchor loss between the two groups.

Clinical significance: The use of i-PRF in orthodontic treatment significantly enhances the rate of space closure and reduces the overall treatment. Injectable platelet-rich fibrin can be a safe adjunct to orthodontic treatment, providing benefits without compromising anchorage stability and aiming to optimize orthodontic treatment efficiency. How to cite this article: Priya SMRP, Anbarasu P, Eisenhuth G, et al. The Impact of Injectable Platelet-rich Fibrin on Orthodontic Tooth Movement during Retraction: A Randomized Controlled Trial. J Contemp Dent Pract 2024;25(9):856-862.

Aim: This study evaluates long-term shear bond strength (SBS) and enamel micro cracks (MCs) healing after using adhesive pre-coated brackets (APC).

Materials and methods: A total of eighty extracted human premolar teeth were randomly divided into four experimental groups (n = 20 per group): Control group: Teeth underwent indentation but no bracket bonding; group II : Teeth were subjected to indentation without exposure to thermocycling; group III: Teeth experienced both indentation and thermocycling; group IV: No indentation was applied to the teeth; groups III and IV were further divided into two subgroups to simulate different clinical timelines: Subgroup A (n = 10): Teeth underwent 5,000 thermocycles, equivalent to six months of clinical use. Subgroup B (n = 10): Teeth were subjected to 10,000 thermocycles, representing 12 months of use. All precoated brackets underwent debonding with a universal testing machine to assess the SBS, and the adhesive remnant index (ARI) was scored to evaluate the amount of adhesive left on the tooth surface. The study also examined horizontal and vertical enamel cracks, both pre- and post-intervention, across all groups. Crack healing was quantitatively assessed using computer-assisted digital image analysis to ensure precision. For statistical evaluation, ANOVA, Kruskal-Wallis H-test, and Tukey's post-hoc tests were applied to assess differences among the groups.

Results: The Kruskal-Wallis H-test demonstrated no significant ARI difference between the groups (p = 0.790). A one-way ANOVA showed a significant difference among all groups (p < 0.001), with lower values observed in the group with indentation without thermocycling compared to all other groups and the groups with 5,000 thermocycles compared to the groups with, 10,000 thermocycles. Crack healing was observed in the control and second groups, and cracks were directly proportional to the number of thermocycles and SBS values.

Conclusion: The study showed that APC FF's SBS increased, and thermal aging did not change the failure pattern. Thermocyclers and SBS affected enamel cracks.

Clinical significance: The bond strength of pre-coated brackets and microcrack healing gradually increased with time, while the pattern of bond failure did not change. How to cite this article: Shamsan H, Albelasy NF, Farahat DS, et al. The Effect of Thermocycling on the Shear Bond Strength of Flash-free Brackets and Healing Dynamics of Enamel Microcracks: An In vitro Study. J Contemp Dent Pract 2024;25(9):836-845.

Aim: To perform a bibliometric study of periodontal disease and Alzheimer's disease (AD) focusing on trends, collaborative efforts, and emerging patterns.

Materials and methods: From January 2018 to May 2024, an observational study was carried out utilizing metadata extracted from the Scopus database. A search methodology, specifically designed for this database, was developed using MeSH terms combined with Boolean operators such as "AND" and "OR". The Bibliometrix tool was employed to facilitate the study, using indicators including the number of citations and publications, the authorship of the publication, the country of origin, the year of publication, the type of publication, the H-index, WorldMap, Sankey diagram, keyword analysis, topic evolution, and scientific findings.

Results: A total of 50.7 and 27.3% of the publications were in Q1 and Q2 quartile journals. The University of Central Lancashire has the most publications (17), but the University of California at San Francisco has the highest impact (1545 citations per publication). The journal Periodontology 2000 had a total of 643 citations per publication. Sim Kaur Singhrao (16) and Ingar Olsen (10) were the authors with the most publications, whereas Eric C Reynolds had the highest impact with 1,300 citations.

Conclusions: This bibliometric study found a gradual growth in publications, with the highest number of studies during 2021 and 2022, followed by a decrease in subsequent years, with Q1 and Q2 journals predominating. In terms of scientific productivity, the United States is the leading country in this area of research. The journal Periodontology 2000 had the highest number of citations, demonstrating the particular interest of periodontology in studying the association between periodontal disease and systemic diseases.

Clinical significance: The present study is significant because the relationship between periodontitis and Alzheimer is currently considered a novel multidisciplinary research opportunity, given that it includes both medical and dental specialties. This research also provides a valuable contribution to academics, universities, and research centers because it will allow us to understand trends and new research horizons and identify the most productive authors. How to cite this article: Matta-Pacheco J, Tsukamoto-Jaramillo A, Tinedo-López PL, et al. Bibliometric Study of Periodontitis and Alzheimer's Disease: Trends, Collaboration, and Emerging Patterns. J Contemp Dent Pract 2024;25(9):863-868.

Aim: The aim of this systematic review was to evaluate the effect of build orientation on the mechanical and physical properties of additively manufactured resin using digital light processing (DLP).

Background: The properties of 3D-printed materials are influenced by various factors, including the type of additive manufacturing (AM) system and build orientation. There is a scarcity of literature on the effect of build orientation on the mechanical and physical properties of additively manufactured resins using DLP technology in dentistry.

Methods: This study followed the preferred reporting items for systematic reviews and meta-analyses (PRISMA) guidelines and was registered in PROSPERO. The formulated population, intervention, comparison, outcome (PICO) question was "What is the effect of build orientation on the mechanical and physical properties of additively manufactured resins produced using DLP in dentistry."  The search strategy used three main electronic databases and an additional manual search was done until February 2024. All the studies that evaluated the correlation of build orientation and the properties of printed resin using DLP were included. Two different analysis was used for in vivo and in vitro studies to assess the risk of bias.

Review results: On search 237 studies were yielded for systematic review, out of which 13 studies were included for the systematic review evaluation. On evaluation and reviewing the included studies, though the build orientation angle influenced the properties of printed resins the results obtained were varied as 90° angle had increased compressive strength, low surface roughness, and best accuracy. The 0° angle had better wear resistance, tensile strength, and high flexural strength. There was no influence of build orientation on microhardness, shear bond strength, gloss and color difference. The studies on denture base showed that 45° build angle showed the truest with best accuracy.

Conclusion: The build orientation angle effects on both the mechanical and physical properties of the additively manufactured resin but varies with each property. The build orientation can be chosen based on the type of properties to be achieved based on the treatment modality.

Clinical significance: Based on the systematic review results the specific build orientation angle should be used during fabrication of any denture designs, crowns, and bridges as it is correlated with the properties to be achieved by particular designs. How to cite this article: Paranna S, Thosar N, Kanitkar A. Effect of Build Orientation on Mechanical and Physical Properties of Additively Manufactured Resins Using Digital Light Processing Technology in Dentistry: A Systematic Review. J Contemp Dent Pract 2024;25(9):891-903.

Aim: The purpose of this study is to investigate the interchangeability of newly acquired, short-term used, and long-term used semi-adjustable articulators.

Materials and methods: Metal analogs of partially dentate maxillary and mandibular arches were mounted on twelve semi-adjustable Stratos 300 articulators, divided into three groups based on usage over time: New, short-term used (<2 years), long-term used (>2 years) articulators. Each articulator was calibrated according to manufacturer guidelines, ensuring consistency. Occlusal contact forces were recorded using a T-scan III device with a pressure-mapping sensor. Maximum intercuspation (MIP) contact magnitudes were recorded by two calibrated examiners at 10-second intervals, ensuring reliable data collection across all conditions.

Results: In MIP, tooth #9 consistently exhibited the highest contact percentages across all articulator conditions, with percentages of 22.2% in the new articulator, 21.8% in the short-term used articulators, and 21.5% in long-term used articulators, indicating stable contact force distribution. Tooth #12 consistently showed the lowest MIP contact percentages, suggesting minimal occlusal force in this region due to its absence. Contact forces, measured at 10-second intervals, showed no significant differences among the different articulator conditions in MIP (p > 0.01).

Conclusion: Within the limitations of this in vitro study, the calibrated Stratos 300 semi-adjustable articulator maintained superior accuracy over time.

Clinical significance: The study confirms that the calibrated Stratos 300 semi-adjustable articulators provide consistent and accurate occlusal measurements over time, regardless of their usage over time. How to cite this article: Almalki A, Kutkut N, Althubaitiy R, et al. Interchangeability of Contemporary Semi-adjustable Articulators Used Over Time: An In Vitro Study. J Contemp Dent Pract 2024;25(9):869-872.