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Structure, Surface Topography, and Glass Transition Temperature of Dental Poly (Methyl Methacrylate) Resin Conjugated with 3,9-bisethenyl-2,4,8,10-tetraoxaspiro [5,5] Undecane as Cross-linker: An In Vitro Research. 以 3,9-双乙烯基-2,4,8,10-四氧杂螺 [5,5] 十一烷为交联剂共轭的牙科聚(甲基丙烯酸甲酯)树脂的结构、表面形貌和玻璃化转变温度:体外研究。
Q3 Dentistry Pub Date : 2024-05-01 DOI: 10.5005/jp-journals-10024-3665
Chandramohan Ravivarman, Ranganathan Ajay, Loganathan Saatwika, Sivarasu Sivasenthil, Thangavelu Saranya, Deepa Gurunathan

Aim and objectives: To characterize and analyze the structural presentation of a new denture base copolymer with a spiro-acetal cross-linker at 10 and 20 wt.% concentrations by nuclear magnetic resonance (NMR) and field emission scanning electron microscopy-energy-dispersive X-ray (FESEM-EDX) spectroscopies. Also, to evaluate the glass transition temperature (TG) of the new copolymer.

Materials and methods: The investigational groups G10 and G20 were heat-cured with the new spiro-acetal cross-linker at the above-mentioned concentrations, respectively. The control group G0 was heat-cured without the new cross-linker. Nuclear magnetic resonance and EDX spectroscopies determined the copolymerization along with elemental composition. The surface characteristics were discerned by FESEM. Differential scanning calorimetry was employed to evaluate the TG of the resultant copolymer. Appropriate statistical operations were performed to compare the mean TG of the groups.

Results: The new copolymer's structure with the spiro-acetal cross-linker was configured with protons, carbons, aluminum, zirconium, yttrium, and silicon atoms. The TG of the resultant copolymer was high when compared with the G0. The 20 wt.% spiro-acetal cross-linker in the copolymer exhibited the highest TG.

Conclusion: The spiro-acetal cross-linking comonomer incorporated in the heat-cure denture polymer produced a new denture base copolymer with elevated TG. The resultant configuration of the new copolymer was characterized, structurally presented, and confirmed.

Clinical significance: The new copolymer might exhibit augmented strength due to the copolymerized spiro-acetal cross-linker. Moreover, the smooth and regular surface of the copolymer would have minimum or negligible microbial adhesion due to the hydrophobicity of the spiro-acetal comonomer incorporated in the denture base composition. How to cite this article: Ravivarman C, Ajay R, Saatwika L, et al. Structure, Surface Topography, and Glass Transition Temperature of Dental Poly (Methyl Methacrylate) Resin Conjugated with 3,9-bisethenyl-2,4,8,10-tetraoxaspiro [5,5] Undecane as Cross-linker: An In Vitro Research. J Contemp Dent Pract 2024;25(5):486-493.

目的和目标:通过核磁共振(NMR)和场发射扫描电子显微镜-能量色散 X 射线(FESEM-EDX)光谱,表征和分析新型义齿基底共聚物与螺醛类交联剂在 10 和 20 wt.% 浓度下的结构表现。此外,还评估了新型共聚物的玻璃化转变温度(TG):研究组 G10 和 G20 分别用上述浓度的新型螺缩醛交联剂进行热固化。对照组 G0 在没有使用新型交联剂的情况下进行热固化。核磁共振和乙二胺四乙酸氙光谱测定了共聚情况和元素组成。通过 FESEM 分析了表面特征。采用差示扫描量热法评估了所得共聚物的热重。通过适当的统计运算来比较各组的平均 TG:结果:带有螺-缩醛交联剂的新型共聚物结构中配置了质子、碳、铝、锆、钇和硅原子。与 G0 相比,所得共聚物的 TG 较高。共聚物中 20 wt.% 的螺-缩醛交联剂的 TG 值最高:结论:在热固化义齿聚合物中加入螺缩醛交联共聚单体可产生一种新的义齿基底共聚物,其 TG 值较高。临床意义:临床意义:由于共聚了螺-缩醛交联剂,这种新型共聚物可能具有更高的强度。此外,由于义齿基托成分中螺-缩醛共聚单体的疏水性,共聚物光滑而规则的表面可将微生物的粘附性降到最低或可忽略不计。本文引用方式Ravivarman C, Ajay R, Saatwika L, et al. Structure, Surface Topography, and Glass Transition Temperature of Dental Poly (Methyl Methacrylate) Resin Conjugated with 3,9-bisethenyl-2,4,8,10-tetraoxaspiro [5,5] Undecane as Cross-linker:体外研究。J Contemp Dent Pract 2024;25(5):486-493.
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引用次数: 0
Comparative Evaluation of Scaling and Root Planing with and without Oxygen-releasing Gel in the Treatment of Chronic Periodontitis: A Split-mouth Study. 使用和不使用释氧凝胶进行洗牙和根面平整治疗慢性牙周炎的比较评估:分口研究
Q3 Dentistry Pub Date : 2024-05-01 DOI: 10.5005/jp-journals-10024-3689
Anuradha Singh, Sanjay Vasudevan, Ajay Reddy Palle, Abhinav Atchuta, Surabhi Bhadauriya

Aim: The study aims to compare the effectiveness of scaling root planing alone and scaling root planing with oxygen-releasing gel in the treatment of chronic periodontitis.

Materials and methods: A split-mouth randomized controlled trial was designed on 25 systemically healthy participants with 50 sites having chronic periodontitis. Two sites were selected for each patient and were randomly allocated into two groups. A total of 50 sites were selected and divided into two groups with 25 test sites in group I (Test group) and 25 control sites in group II (Control group). Group I received scaling and root planing (SRP) followed by placement of BlueM oral gel, while group II received SRP alone. Probing pocket depth (PPD), clinical attachment level (CAL), gingival index (GI), and bleeding on probing (BOP) were recorded at baseline and after 6 weeks and were compared accordingly.

Results: The results revealed a reduction in PPD, CAL, GI, and BOP in both groups. However, the test group showed a statistically significant reduction when compared with the control group in the above-mentioned clinical parameters.

Conclusion: The results suggested that the use of oxygen-releasing gel with SRP provided additional benefits in the management of chronic periodontitis by promoting greater reductions in the clinical parameters.

Clinical significance: BlueM oral gel, i.e. the oxygen-releasing gel as a local drug delivery, may be an excellent adjunct to SRP in treating chronic periodontitis. How to cite this article: Singh A, Vasudevan S, Palle AR, et al. Comparative Evaluation of Scaling and Root Planing with and without Oxygen-releasing Gel in the Treatment of Chronic Periodontitis: A Split-mouth Study. J Contemp Dent Pract 2024;25(5):445-452.

目的:本研究旨在比较单纯洗牙根面和洗牙根面加释氧凝胶治疗慢性牙周炎的效果:设计了一项分口随机对照试验,25 名全身健康的参与者中有 50 个部位患有慢性牙周炎。每位患者选择两个部位,随机分配到两组。共选取 50 个部位,分为两组,25 个试验部位分为 I 组(试验组),25 个对照部位分为 II 组(对照组)。I 组接受洗牙和根面平整(SRP),然后涂抹 BlueM 口腔凝胶,而 II 组仅接受 SRP。记录基线和 6 周后的探诊袋深度(PPD)、临床附着水平(CAL)、牙龈指数(GI)和探诊出血量(BOP),并进行相应的比较:结果显示,两组的 PPD、CAL、GI 和 BOP 均有所下降。然而,与对照组相比,试验组在上述临床参数方面有显著的统计学下降:结果表明,使用含SRP的释氧凝胶能促进临床指标的降低,从而为慢性牙周炎的治疗提供额外的益处:临床意义:BlueM口服凝胶,即作为局部给药的释氧凝胶,可能是治疗慢性牙周炎的SRP的极佳辅助药物。本文引用方式Singh A, Vasudevan S, Palle AR, et al:分口研究》。J Contemp Dent Pract 2024;25(5):445-452.
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引用次数: 0
Effect of Adding Different Concentrations of Silver Nanoparticles on Flexural Strength and Microhardness of Different Denture Base Materials. 添加不同浓度的纳米银对不同义齿基托材料抗弯强度和显微硬度的影响
Q3 Dentistry Pub Date : 2024-05-01 DOI: 10.5005/jp-journals-10024-3688
Ibrahim Gamal El-Hussein

Aim: This study aimed to evaluate the effect of adding different concentrations of silver nanoparticles (AgNPs) on the flexural strength and microhardness of various denture base materials.

Materials and methods: For this study, a total of 60 specimens were used and divided into equal groups. The first group consisted of heat-cured acrylic resin (Vertex-Germany), while the second group consisted of thermoplastic resin (Breflex 2nd edition, Germany). The samples were created using a split brass mold with dimensions of 65 × 10 × 2.5 mm, in accordance with the specifications of the American Dental Association (specifically No. 12 for flexural and microhardness). Following this, the samples were divided into three groups (A, B, and C) based on different concentrations of AgNPs (0, 2, and 5%). The flexural and microhardness of the samples were assessed using a universal testing machine and the Vickers hardness test, respectively. The data were gathered, organized, and analyzed using statistical methods.

Results: The flexural strength findings showed a significant difference between the two groups. Also, there was a considerable decrease in the average value of the acrylic group as the concentrations of AgNPs rose, while the flexural strength of the thermoplastic group notably improved. Regarding microhardness, the results showed a significant difference between the two groups. It showed that the mean value of both groups increased with increasing concentrations of AgNPs.

Conclusion: Within the limitations of laboratory testing conditions of this study, it was discovered that AgNPs negatively impact the flexural strength of acrylic resins. Furthermore, an increase in the concentration of AgNPs was found to be directly related to the flexural strength of thermoplastic resin and the microhardness of both groups.

Clinical significance: The concentration of AgNPs has a significant impact on certain mechanical properties of denture base materials, but it is important to consider their potential toxicity. How to cite this article: El-Hussein IG. Effect of Adding Different Concentrations of Silver Nanoparticles on Flexural Strength and Microhardness of Different Denture Base Materials. J Contemp Dent Pract 2024;25(5):417-423.

目的:本研究旨在评估添加不同浓度的纳米银粒子(AgNPs)对各种义齿基托材料抗弯强度和显微硬度的影响:本研究共使用了 60 个试样,并将其平均分为两组。第一组为热固化丙烯酸树脂(德国 Vertex 公司),第二组为热塑性树脂(德国 Breflex 第二版)。根据美国牙科协会的规范(特别是关于弯曲和微硬度的第 12 号规范),使用尺寸为 65 × 10 × 2.5 毫米的分体式黄铜模具制作样品。然后,根据 AgNPs 的不同浓度(0、2 和 5%)将样品分为三组(A、B 和 C)。分别使用万能试验机和维氏硬度试验对样品的弯曲度和显微硬度进行评估。采用统计方法收集、整理和分析数据:结果:两组样品的抗弯强度差异显著。此外,随着 AgNPs 浓度的增加,丙烯酸组的平均值大幅下降,而热塑性塑料组的抗折强度则明显提高。在显微硬度方面,结果显示两组之间存在显著差异。结论:本研究在实验室测试条件的限制下发现,AgNPs 对丙烯酸树脂的抗弯强度有负面影响。此外,研究还发现 AgNPs 浓度的增加与热塑性树脂的抗折强度和两组的显微硬度直接相关:AgNPs的浓度对义齿基底材料的某些机械性能有显著影响,但必须考虑其潜在毒性。如何引用本文:El-Hussein IG.添加不同浓度的银纳米颗粒对不同义齿基托材料挠曲强度和显微硬度的影响。J Contemp Dent Pract 2024;25(5):417-423.
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引用次数: 0
Assessment of the Efficacy and Bond Strength of Different Dentin-bonding Agents with Adhesives on Primary Teeth: An In Vitro Study. 评估不同牙本质粘接剂与粘合剂在基牙上的功效和粘接强度:体外研究
Q3 Dentistry Pub Date : 2024-04-01 DOI: 10.5005/jp-journals-10024-3658
Adel S Alqarni, Abdulhamid Al Ghwainem

Aim: The purpose of this study was to evaluate the effectiveness and strength of three various dentin-bonding agents used with adhesives on primary teeth.

Materials and methods: The study used 80 recently extracted, healthy human maxillary anterior primary teeth that had undergone physiologic resorption, or over-retention. Teeth were cut to expose a flat dentin surface at a depth of 1.5 mm. All samples were divided into four groups (20 samples in each group) as follows: Group I-Control group, Group II-Primary teeth bonding with 6th-generation bonding agent, Group III-Primary teeth bonding with 7th-generation bonding agent, Group IV-Primary teeth bonding with 8th-generation bonding agent. All of the samples' dentinal surfaces were covered with composite resin using a Teflon mold after adhesive had been applied. A universal testing machine (INSTRON) was used to assess the shear bond strength. Data were collected and statistically analyzed.

Results: The maximum mean shear bond strength was found in 8th-generation bonding agent (30.76 ± 0.16), followed by 7th-generation bonding agent (26.08 ± 0.21), 6th-generation bonding agent (25.32 ± 0.06), and control group (6.18 ± 0.09). Statistically significant difference was found between the three different bonding agents (p < 0.001).

Conclusion: On conclusion, the 8th-generation bonding agent demonstrated a greater shear bond strength to dentin than the 7th and 6th-generation bonding agent.

Clinical significance: The emergence of different bonding techniques to the market improves the durability and quality of restorations. An effective bonding to the tooth would also reduce bacterial penetration, marginal microleakage, possibility of pulpal inflammation preserve tooth structure, and postoperative sensitivity by allowing less cavity preparation. How to cite this article: Alqarni AS, Al Ghwainem A. Assessment of the Efficacy and Bond Strength of Different Dentin-bonding Agents with Adhesives on Primary Teeth: An In Vitro Study. J Contemp Dent Pract 2024;25(4):342-345.

目的:本研究的目的是评估三种不同的牙本质粘结剂与粘合剂一起用于基牙的有效性和强度:研究使用了 80 颗新近拔出的、健康的人类上颌前基牙,这些牙齿都经历了生理性吸收或过度滞留。对牙齿进行切割,暴露出深度为 1.5 毫米的平坦牙本质表面。所有样本分为以下四组(每组 20 个样本):第一组-对照组,第二组-使用第六代粘接剂粘接的基牙,第三组-使用第七代粘接剂粘接的基牙,第四组-使用第八代粘接剂粘接的基牙。所有样本的牙本质表面在涂抹粘合剂后都用特氟隆模具覆盖了复合树脂。使用万能试验机(INSTRON)评估剪切粘接强度。收集数据并进行统计分析:结果:第八代粘接剂的平均剪切粘接强度最大(30.76 ± 0.16),其次是第七代粘接剂(26.08 ± 0.21)、第六代粘接剂(25.32 ± 0.06)和对照组(6.18 ± 0.09)。三种不同粘接剂之间的差异具有统计学意义(P < 0.001):结论:第八代粘接剂与牙本质的剪切粘接强度高于第七代和第六代粘接剂:临床意义:市场上出现的各种粘接技术提高了修复体的耐用性和质量。与牙齿的有效粘接还能减少细菌渗透、边缘微渗漏、牙髓发炎的可能性,保护牙齿结构,并通过减少牙洞预备来降低术后敏感性。本文引用方式Alqarni AS, Al Ghwainem A.不同牙本质粘接剂与粘接剂在基牙上的功效和粘接强度评估:体外研究。J Contemp Dent Pract 2024;25(4):342-345.
{"title":"Assessment of the Efficacy and Bond Strength of Different Dentin-bonding Agents with Adhesives on Primary Teeth: An <i>In Vitro</i> Study.","authors":"Adel S Alqarni, Abdulhamid Al Ghwainem","doi":"10.5005/jp-journals-10024-3658","DOIUrl":"https://doi.org/10.5005/jp-journals-10024-3658","url":null,"abstract":"<p><strong>Aim: </strong>The purpose of this study was to evaluate the effectiveness and strength of three various dentin-bonding agents used with adhesives on primary teeth.</p><p><strong>Materials and methods: </strong>The study used 80 recently extracted, healthy human maxillary anterior primary teeth that had undergone physiologic resorption, or over-retention. Teeth were cut to expose a flat dentin surface at a depth of 1.5 mm. All samples were divided into four groups (20 samples in each group) as follows: Group I-Control group, Group II-Primary teeth bonding with 6th-generation bonding agent, Group III-Primary teeth bonding with 7th-generation bonding agent, Group IV-Primary teeth bonding with 8th-generation bonding agent. All of the samples' dentinal surfaces were covered with composite resin using a Teflon mold after adhesive had been applied. A universal testing machine (INSTRON) was used to assess the shear bond strength. Data were collected and statistically analyzed.</p><p><strong>Results: </strong>The maximum mean shear bond strength was found in 8th-generation bonding agent (30.76 ± 0.16), followed by 7th-generation bonding agent (26.08 ± 0.21), 6th-generation bonding agent (25.32 ± 0.06), and control group (6.18 ± 0.09). Statistically significant difference was found between the three different bonding agents (<i>p</i> < 0.001).</p><p><strong>Conclusion: </strong>On conclusion, the 8th-generation bonding agent demonstrated a greater shear bond strength to dentin than the 7th and 6th-generation bonding agent.</p><p><strong>Clinical significance: </strong>The emergence of different bonding techniques to the market improves the durability and quality of restorations. An effective bonding to the tooth would also reduce bacterial penetration, marginal microleakage, possibility of pulpal inflammation preserve tooth structure, and postoperative sensitivity by allowing less cavity preparation. How to cite this article: Alqarni AS, Al Ghwainem A. Assessment of the Efficacy and Bond Strength of Different Dentin-bonding Agents with Adhesives on Primary Teeth: An <i>In Vitro</i> Study. J Contemp Dent Pract 2024;25(4):342-345.</p>","PeriodicalId":35792,"journal":{"name":"Journal of Contemporary Dental Practice","volume":"25 4","pages":"342-345"},"PeriodicalIF":0.0,"publicationDate":"2024-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141493682","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Evaluation of Mesiodistal Angulation of Maxillary Anterior Teeth in Incisal View Using Manual and Digital Methods: An In Vivo Study. 使用手动和数字方法评估上颌前牙切迹视图中的中轴角度:活体研究
Q3 Dentistry Pub Date : 2024-04-01 DOI: 10.5005/jp-journals-10024-3651
Shashidhara H Shadaksharappa, Banibrata Lahiri, Akshata G Kamath, Ashika B Kashi, Narendra V Penumatsa, Upasana Reddy, Malak S Bamigdad, Rehab M Alwakeb

Aim: The aim of the present research was to assess the mesiodistal angulation of the maxillary anterior teeth utilizing Image J computer software, a Profile projector, and a Custom-made jig.

Materials and methods: A total of 34 subjects (17 males and 17 females) were chosen from a group of 18-30 years old with bilateral Angle Class I molars and canine relationships. One manual approach (Custom-made jig) and two digital methods (J computer software, a Profile projector) were used to record the mesiodistal angulation in incisal view. The individuals had alginate impressions made, and a facebow was used to capture the maxilla's spatial relationship with the cranium. The articulated cast with the help of mounting ring moved to the specially customized jig, then the angulations was measured in the incisal view after the casts were placed in a semi-adjustable articulator. Data were recorded and statistically analyzed.

Results: The mesiodistal angulation in the incisal view via three methods between the 17 males and 17 females has statistically significant different. Although the mesiodistal angulation for maxillary lateral incisor and canine did not show any statistically significant difference, the maximum and minimum values obtained were always greater in males in comparison with the females. This indicates that the positions of six maxillary anterior teeth in the males resulted in the creation of upward sweep of incisal edges of central and lateral incisors which was also referred to as "smiling line" producing masculine surface anatomy more squared and vigorous while feminine surface anatomy being more rounded, soft, and pleasant. There was no statistically significant difference between the right and left sides, indicating bilateral arch symmetry and the symmetrical place of the right teeth compared with the left side's corresponding teeth.

Conclusion: On conclusion, according to the current study's findings, all three approaches can measure the mesiodistal angulations of maxillary anterior teeth in incisal view with clinically acceptable accuracy. The digital methods, which included using the Image J computer software and the profile projector, achieved more accurate results than the manual method.

Clinical significance: The outcomes of this study's mesiodistal angulations can be used as a reference for placing teeth in both fully and partially edentulous conditions. This study contributes to a better understanding of the importance of achieving the ideal occlusion in the Indian population by placing the maxillary anterior teeth at the proper mesiodistal angulation. How to cite this article: Shadaksharappa SH, Lahiri B, Kamath AG, et al. Evaluation of Mesiodistal Angulation of Maxillary Anterior Teeth in Incisal View Using Manual and Digital Methods: An In Vivo Study. J Contemp Dent Pract 2024;25(4):320-325.

目的:本研究的目的是利用 Image J 计算机软件、轮廓投影仪和定制夹具评估上颌前牙的齿间角:从 18-30 岁的双侧角Ⅰ类臼齿和犬齿关系群体中选取了 34 名受试者(17 男 17 女)。使用一种手动方法(定制夹具)和两种数字方法(J 计算机软件、轮廓投影仪)记录切缘视图中的牙间隙角度。为每个人制作藻酸盐印模,并使用面弓捕捉上颌骨与颅骨的空间关系。在安装环的帮助下,将铰接好的铸模移到专门定制的夹具上,然后将铸模放入半可调铰接器中,在切面上测量角度。记录数据并进行统计分析:结果:17 名男性和 17 名女性通过三种方法测量的牙间隙角度在统计学上有显著差异。虽然上颌侧切牙和犬齿的中轴角度在统计学上没有明显差异,但男性获得的最大值和最小值总是大于女性。这表明男性六颗上颌前牙的位置导致中切牙和侧切牙的切缘向上扫过,这也被称为 "微笑线",使男性的表面解剖更方正、有力,而女性的表面解剖则更圆润、柔和、宜人。左右两侧的差异没有统计学意义,这表明双侧牙弓对称,右侧牙齿的位置与左侧相应牙齿的位置对称:总之,根据目前的研究结果,这三种方法都能在切面上测量上颌前牙的中轴角度,其准确性在临床上是可以接受的。使用 Image J 计算机软件和轮廓投影仪的数字化方法比手动方法获得的结果更准确:临床意义:这项研究的结果可作为全口和部分缺牙情况下镶牙的参考。这项研究有助于更好地理解在印度人群中通过将上颌前牙置于适当的中轴角度来实现理想咬合的重要性。如何引用本文:Shadaksharappa SH, Lahiri B, Kamath AG, et al:活体研究。J Contemp Dent Pract 2024;25(4):320-325.
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引用次数: 0
Regenerative Potential of Dental Pulp Stem Cells in Response to a Bioceramic Dental Sealer and Photobiomodulation: An In Vitro Study. 牙髓干细胞对生物陶瓷牙齿封闭剂和光生物调节的再生潜力:体外研究
Q3 Dentistry Pub Date : 2024-04-01 DOI: 10.5005/jp-journals-10024-3676
Hamed A Alshawkani, Mohamed Mansy, Mahmoud Al Ankily, Mohamed Shamel

Aims: This study aims to assess the synergistic effect of utilizing a bioceramic sealer, NeoPutty, with photobiomodulation (PBM) on dental pulp stem cells (DPSCs) for odontogenesis.

Materials and methods: Dental pulp stem cells were collected from 10 premolars extracted from healthy individuals. Dental pulp stem cells were characterized using an inverted-phase microscope to detect cell shape and flow cytometry to detect stem cell-specific surface antigens. Three experimental groups were examined: the NP group, the PBM group, and the combined NP and PBM group. A 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) experiment was conducted to assess the viability of DPSCs. The odontogenic differentiation potential was analyzed using Alizarin red staining, RT-qPCR analysis of odontogenic genes DMP-1, DSPP, and alkaline phosphatase (ALP), and western blot analysis for detecting BMP-2 and RUNX-2 protein expression. An analysis of variance (ANOVA) followed by a post hoc t-test was employed to examine and compare the mean values of the results.

Results: The study showed a notable rise in cell viability when NP and PBM were used together. Odontogenic gene expression and the protein expression of BMP-2 and RUNX-2 were notably increased in the combined group. The combined effect of NeoPutty and PBM was significant in enhancing the odontogenic differentiation capability of DPSCs.

Conclusion: The synergistic effect of NeoPutty and PBM produced the most positive effect on the cytocompatibility and odontogenic differentiation potential of DPSCs.

Clinical significance: Creating innovative regenerative treatments to efficiently and durably repair injured dental tissues. How to cite this article: Alshawkani HA, Mansy M, Al Ankily M, et al. Regenerative Potential of Dental Pulp Stem Cells in Response to a Bioceramic Dental Sealer and Photobiomodulation: An In Vitro Study. J Contemp Dent Pract 2024;25(4):313-319.

目的:本研究旨在评估使用生物陶瓷密封剂 NeoPutty 和光生物调制(PBM)对牙髓干细胞(DPSCs)进行牙体生成的协同效应:从健康人的 10 颗前臼齿中采集牙髓干细胞。使用倒置相显微镜检测细胞形状,并使用流式细胞术检测干细胞特异性表面抗原,对牙髓干细胞进行特征描述。实验分为三组:NP组、PBM组以及NP和PBM联合组。3-(4,5-二甲基噻唑-2-基)-2,5-二苯基溴化四氮唑(MTT)实验用于评估DPSCs的活力。利用茜素红染色、牙源基因 DMP-1、DSPP 和碱性磷酸酶(ALP)的 RT-qPCR 分析以及检测 BMP-2 和 RUNX-2 蛋白表达的 Western 印迹分析,对牙源性分化潜能进行了分析。采用方差分析(ANOVA)和事后t检验来检验和比较结果的平均值:研究结果表明,当 NP 和 PBM 同时使用时,细胞活力明显提高。联合组的牙源性基因表达以及 BMP-2 和 RUNX-2 蛋白表达明显增加。NeoPutty 和 PBM 的联合作用在增强 DPSCs 的牙源性分化能力方面效果显著:结论:NeoPutty 和 PBM 的协同作用对 DPSCs 的细胞相容性和牙本质分化潜能产生了最积极的影响:临床意义:创造创新的再生疗法,高效持久地修复损伤的牙组织。本文引用方式Alshawkani HA, Mansy M, Al Ankily M, et al:体外研究。J Contemp Dent Pract 2024;25(4):313-319.
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引用次数: 0
Evaluation of Microleakage of Nanoparticle-incorporated Cyanoacrylate Root Canal Sealer Using the Radioisotopic Method: An In Vitro Study. 使用放射性同位素方法评估纳米颗粒掺入的氰基丙烯酸酯根管封闭剂的微渗漏:体外研究
Q3 Dentistry Pub Date : 2024-04-01 DOI: 10.5005/jp-journals-10024-3683
Chitharanjan Shetty, Shazeena Qaiser, Aditya Shetty, Rashi Shroff

Aim of the study: The study aimed to assess the microleakage of nanoparticle-based (NPB) cyanoacrylate sealer and epoxy resin-based (ERB) sealer using radioisotope method and confocal laser scanning microscopy (CLSM).

Materials and methods: A total of 100 single-rooted teeth were collected; specimens were accessed, instrumented, and irrigated, and randomly distributed into 4 groups of 25 samples each: Group I: Positive control, group II: Negative control, group III: Obturated with NPB sealer, and group IV: Obturated with ERB sealer. All samples were immersed in 99mTc pertechnetate solution, for 3 hours after which radioactivity was estimated under a Gamma camera. The radioactivity released by specimens before and after nail varnish removal was statistically analyzed. After 2 weeks, the same samples were used for CLSM analysis. The sealer tubular penetration depth was measured at the deepest level for each group using ZEN lite 2012. Data collected was statistically evaluated.

Results: The amount of radioactivity observed at first in group III and group IV was 194.76 and 599.12 units, respectively, with p-value < 0.001, indicating significant interaction, and after nail varnish removal, it was 89.68 and 468.44 units, respectively, with a p-value < 0.001; again, indicating statistical significance. Hence, the radioactivity of NPB sealer was found to be lower than ERB sealer in both cases, indicating better sealing of the former. The photomicrographs show that mean value of dye penetration in NPB sealer in first, second, and third segment from apex was 85.06, 75.73, and 66.09, respectively; while in the case of ERB sealer, those were 597.28, 461.17, and 195.68, respectively; with p-value < 0.001; signifying that NPB sealer exhibited higher resistance to microleakage than ERB sealer.

Conclusion: The NPB sealer can become a potential root canal sealer in future endodontics due to superior physiochemical properties attributed to the cyanoacrylate and incorporated nanoparticles.

Clinical significance: The study clinically signifies that we can equally use the radioisotopic method along with confocal method while conducting the microleakage studies. In addition, NPB sealer can be an emerging replacement with better properties than gold standard root canal sealers for clinical use. How to cite this article: Shetty C, Qaiser S, Shetty A, et al. Evaluation of Microleakage of Nanoparticle-incorporated Cyanoacrylate Root Canal Sealer Using the Radioisotopic Method: An In Vitro Study. J Contemp Dent Pract 2024;25(4):335-341.

研究目的该研究旨在使用放射性同位素方法和共聚焦激光扫描显微镜(CLSM)评估纳米粒子基(NPB)氰基丙烯酸酯封闭剂和环氧树脂基(ERB)封闭剂的微渗漏情况:共收集 100 颗单根牙齿,对标本进行取材、器械检查和冲洗,并随机分为 4 组,每组 25 个标本:第一组:阳性对照;第二组:阴性对照;第三组:用 NPB 封闭剂封闭;第四组:用 ERB 封闭剂封闭。所有样本都浸泡在 99mTc 过硫酸盐溶液中 3 小时,然后在伽马相机下估算放射性。对去除指甲油前后样本释放的放射性进行统计分析。2 周后,同样的样本用于 CLSM 分析。使用 ZEN lite 2012 在每组的最深处测量封层管的渗透深度。对收集到的数据进行统计评估:结果:第三组和第四组最初观察到的放射性分别为 194.76 和 599.12 单位,P 值<0.001,表明存在显著的交互作用;去除指甲油后,分别为 89.68 和 468.44 单位,P 值<0.001;同样表明存在统计学意义。因此,在这两种情况下,NPB 密封剂的放射性都低于 ERB 密封剂,表明前者的密封性更好。显微照片显示,NPB 密封剂在第一、第二和第三段距牙尖的染料渗透平均值分别为 85.06、75.73 和 66.09;而 ERB 密封剂则分别为 597.28、461.17 和 195.68;P 值均小于 0.001;这表明 NPB 密封剂比 ERB 密封剂具有更强的抗微渗漏能力:结论:由于氰基丙烯酸酯和纳米颗粒具有优异的理化特性,NPB封闭剂可以成为未来根管治疗中一种潜在的根管封闭剂:这项研究在临床上表明,在进行微渗漏研究时,我们可以同时使用放射性同位素方法和共聚焦方法。此外,NPB封闭剂可作为一种新兴的替代品,其性能优于金标准根管封闭剂,可供临床使用。本文引用方式Shetty C, Qaiser S, Shetty A, et al:体外研究。J Contemp Dent Pract 2024;25(4):335-341.
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引用次数: 0
Evaluation of the Smear Layer Removal Ability of Various Rotary Files with/without Chemical Agents on Primary Teeth: An In Vitro Study. 评估含/不含化学制剂的各种旋转锉在基牙上去除污垢层的能力:体外研究
Q3 Dentistry Pub Date : 2024-04-01 DOI: 10.5005/jp-journals-10024-3657
AlWaleed Abushanan

Aim: The aim of the current study was to assess the cleaning and smear layer removal efficacy of two different rotary files with or without chemical agents on primary teeth.

Materials and methods: For the study, 90 extracted primary maxillary incisors without internal or external resorption and with at least two-thirds of complete roots were chosen. Then, based on the kind of instruments used to clean and shape the canals, they were randomly assigned to three experimental groups, each consisting of 30 teeth. Group-I: The canal was instrumented manually with K-files, Group-II: The canal was instrumented with Kedo-S files, Group-III: The canal was instrumented with Kedo-SG Blue files. After the canals were finally instrumented, 2 mL of QMixTM solution was used to irrigate 15 samples from each group. The samples were subsequently allowed to remain in the canals for 90 seconds in order to eliminate the smear layer. After that a stereomicroscope was used to assess the cleaning effectiveness.

Results: With irrigant solution, the highest mean value was found in manual K-files (2.86 ± 0.34), followed by Kedo-S files group (1.34 ± 0.26) and Kedo-SG Blue files (1.28 ± 0.18). Without irrigant solution, the highest mean value was found in manual K-files (2.92 ± 0.22) followed by Kedo-S files group (1.44 ± 0.18) and Kedo-SG Blue files (1.36 ± 0.14). There was a statistically significant difference found at all the three levels.

Conclusion: On conclusion, the current study's findings demonstrated that irrigation solution was significantly more effective in cleaning and removing smear layers from pediatric rotary files than manual K-files.

Clinical significance: The effectiveness of endodontic therapy depends on a successful chemomechanical preparation. The canals are instrumented using either hand files or rotary instruments; there are several irrigation and instrumentation techniques. In order to completely sterilize the canals, chemical agents are utilized for irrigation during instrumentation. Due to their numerous biological, antibacterial, anti-inflammatory, and antioxidant qualities, many natural compounds are also utilized as irrigants. How to cite this article: Abushanan A. Evaluation of the Smear Layer Removal Ability of Various Rotary Files with/without Chemical Agents on Primary Teeth: An In Vitro Study. J Contemp Dent Pract 2024;25(4):354-357.

目的:本研究旨在评估两种不同的旋转锉(含或不含化学制剂)对基牙的清洁和去污效果:研究选择了 90 颗拔出的上颌原切牙,这些牙齿没有内外吸收,且至少有三分之二的完整牙根。然后,根据清洁和塑造牙管所用器械的种类,将它们随机分配到三个实验组,每组 30 颗牙齿。第一组:使用 K 型锉对牙根管进行手动器械操作;第二组:使用 Kedo-S 型锉对牙根管进行器械操作;第三组:使用 Kedo-SG Blue 型锉对牙根管进行器械操作。最后,在对根管进行器械操作后,用 2 毫升 QMixTM 溶液对每组的 15 个样本进行灌洗。然后让样本在根管内停留 90 秒,以消除涂抹层。然后使用体视显微镜评估清洁效果:使用冲洗液时,手动 K 型锉的平均值最高(2.86 ± 0.34),其次是 Kedo-S 型锉组(1.34 ± 0.26)和 Kedo-SG 蓝色锉(1.28 ± 0.18)。在不使用冲洗液的情况下,手动 K 型锉的平均值最高(2.92 ± 0.22),其次是 Kedo-S 型锉组(1.44 ± 0.18)和 Kedo-SG Blue 型锉(1.36 ± 0.14)。所有三个级别在统计学上都有显著差异:总之,目前的研究结果表明,冲洗溶液在清洁和去除小儿旋转锉涂抹层方面的效果明显优于手动K锉:牙髓治疗的有效性取决于成功的化学机械准备。使用手动锉或旋转器械对根管进行器械处理;有多种灌洗和器械处理技术。为了彻底消毒根管,在器械操作过程中需要使用化学药剂进行冲洗。由于许多天然化合物具有生物、抗菌、消炎和抗氧化特性,因此也被用作灌洗剂。如何引用本文:Abushanan A. Evaluation of the Smear Layer Removal Abushanan A. Evaluation of the Smear Layer Removal Abushanan A. Evaluation of the Smear Layer Removal Abushanan A. Evaluation of Various Rotary Files with/without Chemical Agents on Primary Teeth:体外研究。J Contemp Dent Pract 2024;25(4):354-357.
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引用次数: 0
Status of Research on Dental Caries during Pregnancy: A Biometric Exploration. 孕期龋齿研究现状:生物计量学探索。
Q3 Dentistry Pub Date : 2024-04-01 DOI: 10.5005/jp-journals-10024-3686
Martha Muñoz-Hidalgo, Yenny Verastegui-Cabanillas, John Barja-Ore

Aim: To describe the bibliometric characteristics of the state of scientific production on dental caries during pregnancy.

Materials and methods: A bibliometric study with a sample of 408 publications indexed in the Scopus database, which were selected based on a search strategy that included logical operators and the term MESH. The Scival tool was used to analyze the metadata.

Results: Jamieson Lisa Marie is positioned as the author with the most publications, and Schroth Robert as the one with the best-weighted impact (FWCI: 37.7). High-impact journals such as BMC Oral Health, Journal of Dental Research, and BMC Public Health stand out for their productivity and an average number of citations. The United States has a large number of publications and evidence of networks of scientific activity with Australia, Canada, and Brazil. The University of Adelaide leads the production and the work of two Latin American institutions (Universidade de São Paulo and Universidade Federal do Maranhão) stands out. International collaboration has improved during the study period.

Conclusion: Scientific production on dental caries during pregnancy is increasingly published in high-impact journals, with growing international collaboration. The United States leads in publications, while Australian institutions are the most productive in this field.

Clinical significance: Caries during pregnancy is a problem that has an important impact on maternal and perinatal health; thus, its timely management and prevention are important areas of research in the field of oral health. How to cite this article: Muñoz-Hidalgo M, Verastegui-Cabanillas Y, Barja-Ore J. Status of Research on Dental Caries during Pregnancy: A Biometric Exploration. J Contemp Dent Pract 2024;25(4):386-391.

目的:描述有关孕期龋齿的科研成果的文献计量学特征:以Scopus数据库收录的408篇出版物为样本进行文献计量学研究,这些出版物是根据包括逻辑运算符和MESH一词的检索策略筛选出来的。使用 Scival 工具分析元数据:结果:Jamieson Lisa Marie 是发表论文最多的作者,Schroth Robert 是加权影响力最大的作者(FWCI:37.7)。BMC 口腔卫生》、《牙科研究杂志》和《BMC 公共卫生》等高影响力期刊因其生产力和平均引用次数而脱颖而出。美国发表了大量论文,并与澳大利亚、加拿大和巴西建立了科学活动网络。阿德莱德大学在论文发表方面处于领先地位,两所拉丁美洲院校(圣保罗大学和马拉尼昂联邦大学)的工作也很突出。在研究期间,国际合作得到了加强:关于孕期龋齿的科研成果越来越多地发表在影响力较大的期刊上,国际合作也日益增多。美国在该领域的论文发表量居首位,而澳大利亚机构在该领域的论文发表量最高:临床意义:妊娠期龋齿是一个对孕产妇和围产期健康有重要影响的问题;因此,及时处理和预防龋齿是口腔健康领域的重要研究领域。如何引用本文:Muñoz-Hidalgo M, Verastegui-Cabanillas Y, Barja-Ore J. Status of Research on Dental Caries during Pregnancy:生物统计学探索。J Contemp Dent Pract 2024;25(4):386-391.
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引用次数: 0
Assessment of Smear Layer Removal and Penetration Depth of Root Canal Irrigant Using Different Irrigation Activation Systems: A Comparative Study. 使用不同冲洗活化系统对根管冲洗剂涂抹层去除和渗透深度的评估:比较研究
Q3 Dentistry Pub Date : 2024-04-01 DOI: 10.5005/jp-journals-10024-3626
Mohan D Pujari, Maneesha Das, Asutosh Das, Dinesh G Kamath, Junu Henry, Arun Shyam, Doaa M Alhaleis

Aim: The aim of the current study was to evaluate the penetration depth and smear layer removal of root canal irrigant using various irrigation activation techniques.

Materials and methods: In this investigation, sixty single-rooted premolars extracted for orthodontic purposes were chosen. Diamond burs were used to create an access cavity, and #10 K-file was used to determine the patency. About sixty samples were divided into the following three groups (20 samples in each group), group I: Irrigation with conventional needle, group II: Activation of EndoVac system, group III: Passive ultrasonic irrigation (PUI). The efficacy of the smear layer was assessed using a scanning electron microscopy at a ×2000 magnification. One-way ANOVA was used to record and analyze the data. All statistical analyses were performed with a significance level of p < 0.05.

Results: At coronal third, the maximum smear layer was removed in group II (1.26 ± 0.02) followed by group III (1.84 ± 0.16) and group I (2.89 ± 0.21). At middle third, smear layer removal was maximum in group I (1.18 ± 0.10) followed by group III (1.72 ± 0.09) and group I (2.66 ± 0.18). At apical third, the more smear layer was removed in group II (1.02 ± 0.01) followed by group III (1.58 ± 0.08) and group I (2.38 ± 0.06). There was a highly significant difference found between the three different irrigation systems at all three levels (p < 0.001).

Conclusion: In conclusion, every irrigation device that was evaluated was successful in removing the smear layer from the root canal. However, the EndoVac system group removed a greater amount of smear layer compared with PUI and conventional needle group.

Clinical significance: With the goal of promoting cleaning that is beyond the ability of mechanical devices, irrigation is a crucial part of root canal therapy. If an efficient irrigation delivery system is used, the irrigants can reach the working length (WL). This type of distribution system needs to provide a suitable amount of irrigants up to the WL, as well as have enough flow and be effective at debriding the entire canal system. How to cite this article: Pujari MD, Das M, Das A, et al. Assessment of Smear Layer Removal and Penetration Depth of Root Canal Irrigant Using Different Irrigation Activation Systems: A Comparative Study. J Contemp Dent Pract 2024;25(4):331-334.

目的:本研究旨在评估根管冲洗剂在使用各种冲洗激活技术时的渗透深度和涂抹层去除情况:本次研究选择了六十颗因正畸而拔出的单根前臼齿。使用金刚石车针创建通路洞,使用 10 号 K 型锉确定通畅性。约 60 个样本被分为以下三组(每组 20 个样本):第一组:传统针头灌洗;第二组:激活 EndoVac 系统;第三组:被动超声波灌洗(PUI)。涂片层的效果使用 ×2000 倍率的扫描电子显微镜进行评估。采用单因素方差分析来记录和分析数据。所有统计分析的显著性水平均为 p <0.05:在冠状三分之一处,第二组(1.26 ± 0.02)去除的涂片层最多,其次是第三组(1.84 ± 0.16)和第一组(2.89 ± 0.21)。在中三分之一处,涂片层去除最多的是 I 组(1.18 ± 0.10),其次是 III 组(1.72 ± 0.09)和 I 组(2.66 ± 0.18)。在根尖三分之一处,第二组(1.02 ± 0.01)去除的涂片层最多,其次是第三组(1.58 ± 0.08)和第一组(2.38 ± 0.06)。三种不同的灌洗系统在所有三个层面上都有非常显著的差异(P < 0.001):总之,所有接受评估的冲洗设备都能成功去除根管中的涂抹层。然而,与 PUI 和传统针头组相比,EndoVac 系统组清除的涂片层更多:临床意义:冲洗的目的是促进机械装置无法达到的清洁效果,因此冲洗是根管治疗的关键部分。如果使用高效的灌洗输送系统,灌洗液就能到达工作长度(WL)。这种灌溉系统需要提供适量的灌溉剂到达工作长度(WL),同时还要有足够的流量,并能有效地对整个根管系统进行清创。如何引用本文:Pujari MD, Das M, Das A, et al:比较研究。J Contemp Dent Pract 2024;25(4):331-334.
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Journal of Contemporary Dental Practice
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