Pub Date : 2018-06-24DOI: 10.33687/PHYTOPATH.007.02.2589
S. Ghuffar, G. Irshad, F. Zhai, Asif Aziz, H. M. Asadullah, N. Mehmood, Hanli Yang, A. Bashir, M. Z. Ahmed, M. Aslam, R. Ahmed
Grapes (Vitis vinifera) are the important fruit crop in Pakistan, mostly cultivated for edible purpose. In September 2016, unusual fruit rot symptoms were observed 3-5 days after harvesting on grapes cv. Kishmishi in post-harvest packing houses in Jehlum district (32°56'22.3"N 73°43'31.4"E) of Punjab province. To determine the disease incidence, a total of 10 boxes of grapes from 5 different locations were selected randomly. Each box contained average 12 bunches and 30 bunches out of 120 inspected bunches displayed typical symptoms of the disease. The initial Symptoms were small, round, water-soaked lesions that rapidly developed into soft, white to light pink mycelium near the centre of infected fruits (Figure 1). A total of 186 symptomatic berries were surface sterilized with 1% sodium hypochlorite, rinsed three times with sterile distilled water and dried by placing on filter paper for 45 sec. Sterilized tissues (approximately 4 mm3) were excised and incubated on potato dextrose agar (PDA) medium at 25 ± 4°C. One week after incubation, colonies with abundant aerial mycelium were initially white, cottony and turned to violet and dark purple with age (Figure 2). A total of 25 isolates were examined morphologically. Macroconidia were slender, thin-walled, 3 to 5 septate, curved apical cell, with 20.9 to 45.2 × 3.2 to 7.1 μm and Microconidia were thin-walled, aseptate, club-shaped with 4.5 to 11.2 × 2.3 to 4.1 μm (Figure 3). These characteristics best fit for the description of Fusarium proliferatum (Leslie and Summerell, 2006). Portions of the internal transcribed spacer (ITS) region were sequenced (White et al., 1990). Sequences of two isolates Fus 07 and Fus 09 (GenBank Accessions; MH444366 and MH464139) showed 100% identity to the corresponding gene sequences of Fusarium proliferatum (GenBank Accessions; MH368119, MF033172 and KU939071) (Figure 4). Pathogenicity test was performed by inoculation with 50-μl conidial suspension (1 × 106conidia/ml) of two isolates onto three non-wounded and four wounded asymptomatic grapes berries. Sterile distilled water was used for a negative control (Figure 5). The experiment was conducted twice and berries were incubated at 25 ± 2°C in sterile moisture chambers (Ghuffar et al., 2018). White to light pink mycelium in appearance with the original symptoms were observed on both wounded and non-wounded inoculated berries after 3 days, whereas no symptoms were observed on the negative control. The morphology of the fungus that was re-isolated from each of the inoculated berries was identical to that of the original cultures. Fusarium proliferatum, one of the destructive species, causes diseases like foot-rot of corn (Farr et al., 1990), root rot of soybean (Díaz Arias et al., 2011), bakanae of rice (Zainudin et al., 2008), wilt of date palm (Khudhair et al., 2014), tomato wilt (Chehri, 2016) and tomato fruit rot (Murad et al., 2016). To our knowledge, this is the first report of Fusarium proliferatum causing frui
{"title":"First Report of Fusarium proliferatum Causing Fruit Rot of Grapes (Vitis vinifera) in Pakistan","authors":"S. Ghuffar, G. Irshad, F. Zhai, Asif Aziz, H. M. Asadullah, N. Mehmood, Hanli Yang, A. Bashir, M. Z. Ahmed, M. Aslam, R. Ahmed","doi":"10.33687/PHYTOPATH.007.02.2589","DOIUrl":"https://doi.org/10.33687/PHYTOPATH.007.02.2589","url":null,"abstract":"Grapes (Vitis vinifera) are the important fruit crop in Pakistan, mostly cultivated for edible purpose. In September 2016, unusual fruit rot symptoms were observed 3-5 days after harvesting on grapes cv. Kishmishi in post-harvest packing houses in Jehlum district (32°56'22.3\"N 73°43'31.4\"E) of Punjab province. To determine the disease incidence, a total of 10 boxes of grapes from 5 different locations were selected randomly. Each box contained average 12 bunches and 30 bunches out of 120 inspected bunches displayed typical symptoms of the disease. The initial Symptoms were small, round, water-soaked lesions that rapidly developed into soft, white to light pink mycelium near the centre of infected fruits (Figure 1). A total of 186 symptomatic berries were surface sterilized with 1% sodium hypochlorite, rinsed three times with sterile distilled water and dried by placing on filter paper for 45 sec. Sterilized tissues (approximately 4 mm3) were excised and incubated on potato dextrose agar (PDA) medium at 25 ± 4°C. One week after incubation, colonies with abundant aerial mycelium were initially white, cottony and turned to violet and dark purple with age (Figure 2). A total of 25 isolates were examined morphologically. Macroconidia were slender, thin-walled, 3 to 5 septate, curved apical cell, with 20.9 to 45.2 × 3.2 to 7.1 μm and Microconidia were thin-walled, aseptate, club-shaped with 4.5 to 11.2 × 2.3 to 4.1 μm (Figure 3). These characteristics best fit for the description of Fusarium proliferatum (Leslie and Summerell, 2006). Portions of the internal transcribed spacer (ITS) region were sequenced (White et al., 1990). Sequences of two isolates Fus 07 and Fus 09 (GenBank Accessions; MH444366 and MH464139) showed 100% identity to the corresponding gene sequences of Fusarium proliferatum (GenBank Accessions; MH368119, MF033172 and KU939071) (Figure 4). Pathogenicity test was performed by inoculation with 50-μl conidial suspension (1 × 106conidia/ml) of two isolates onto three non-wounded and four wounded asymptomatic grapes berries. Sterile distilled water was used for a negative control (Figure 5). The experiment was conducted twice and berries were incubated at 25 ± 2°C in sterile moisture chambers (Ghuffar et al., 2018). White to light pink mycelium in appearance with the original symptoms were observed on both wounded and non-wounded inoculated berries after 3 days, whereas no symptoms were observed on the negative control. The morphology of the fungus that was re-isolated from each of the inoculated berries was identical to that of the original cultures. Fusarium proliferatum, one of the destructive species, causes diseases like foot-rot of corn (Farr et al., 1990), root rot of soybean (Díaz Arias et al., 2011), bakanae of rice (Zainudin et al., 2008), wilt of date palm (Khudhair et al., 2014), tomato wilt (Chehri, 2016) and tomato fruit rot (Murad et al., 2016). To our knowledge, this is the first report of Fusarium proliferatum causing frui","PeriodicalId":36106,"journal":{"name":"International Journal of Phytopathology","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2018-06-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"77950007","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2018-06-24DOI: 10.33687/PHYTOPATH.007.02.2383
M. Mostafa
Potato tubers, under storage conditions, suffer from Fusarium dry rot disease caused by Fusarium spp. leading to devastating losses of stored tubers. Newly harvested tubers are resistance toward Fusarium infection, but disease becomes more vigorous by increasing storage period. Inoculation of potato tuber slices of Desiree cv. (highly susceptible) or Spunta cv. (highly resistant) either by a weak pathogen (F. moniliforme) or severe pathogen (F. solani) and tracking accumulation of phytoalexin rishitin in inoculated tissues at different intervals from inoculation revealed that Desiree cv. accumulated high amounts of rishitin in a very short time in comparison to another three tested situations. Dipping of potato tuber slices of both cvs. in different concentrations of the known antibiotic chloramphenicol prior to inoculation with a weak pathogen (F. moniliforme) led to increased disease severity associated with accumulation huge amounts of rishitin in inoculated tissues. The correlation coefficient between disease severity and rishitin production was +0.83. Moreover, the reaction of spunta cv. toward inoculation by a weak pathogen was changed from the state of resistance to very severe susceptibility. Since chloramphenicol affects protein synthesis on 70S ribosome’s, it was postulated that it prevents synthesis of factor(s) take a part in cell death during infection. Mitochondria extracted from potato tuber tissues secret compound(s) decreased rishitin synthesis in potato tuber slices treated with mycelia extract of F. solani. Treatment of isolated mitochondria with this antibiotic led to the elimination of this factor(s) from their secretions. Results obtained revealed that the elicitation of phytoalexins may consider one of pathogenicity factor of such system.
{"title":"Elicitation of Phytoalexin rishitin in Potato Tuber Slices Infected by Fusarium spp., Does it Consider a Factor of Pathogenicity?","authors":"M. Mostafa","doi":"10.33687/PHYTOPATH.007.02.2383","DOIUrl":"https://doi.org/10.33687/PHYTOPATH.007.02.2383","url":null,"abstract":"Potato tubers, under storage conditions, suffer from Fusarium dry rot disease caused by Fusarium spp. leading to devastating losses of stored tubers. Newly harvested tubers are resistance toward Fusarium infection, but disease becomes more vigorous by increasing storage period. Inoculation of potato tuber slices of Desiree cv. (highly susceptible) or Spunta cv. (highly resistant) either by a weak pathogen (F. moniliforme) or severe pathogen (F. solani) and tracking accumulation of phytoalexin rishitin in inoculated tissues at different intervals from inoculation revealed that Desiree cv. accumulated high amounts of rishitin in a very short time in comparison to another three tested situations. Dipping of potato tuber slices of both cvs. in different concentrations of the known antibiotic chloramphenicol prior to inoculation with a weak pathogen (F. moniliforme) led to increased disease severity associated with accumulation huge amounts of rishitin in inoculated tissues. The correlation coefficient between disease severity and rishitin production was +0.83. Moreover, the reaction of spunta cv. toward inoculation by a weak pathogen was changed from the state of resistance to very severe susceptibility. Since chloramphenicol affects protein synthesis on 70S ribosome’s, it was postulated that it prevents synthesis of factor(s) take a part in cell death during infection. Mitochondria extracted from potato tuber tissues secret compound(s) decreased rishitin synthesis in potato tuber slices treated with mycelia extract of F. solani. Treatment of isolated mitochondria with this antibiotic led to the elimination of this factor(s) from their secretions. Results obtained revealed that the elicitation of phytoalexins may consider one of pathogenicity factor of such system.","PeriodicalId":36106,"journal":{"name":"International Journal of Phytopathology","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2018-06-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"81206868","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2018-06-24DOI: 10.33687/phytopath.007.02.2593
Kablan Kan Aurore Bel Martine, S. Diabaté, K. J. Noel, K. Hilaire, Koné Mongomaké
The oil palm (Elaeis guineensis Jacq.) genetic improvement is based on a recurrent reciprocal selection scheme, involving two groups of populations whose production components are complementary. Group A is essentially coming from Asia, while group B includes African oil palm populations. In order to increase genetic variability and to enrich the agronomic qualities of this group B, a survey was carried out in Western Côte d'Ivoire. Twelve traditional genotypes were collected in the Man area. They are known to produce a rather fluid palm oil but their behavior against Fusarium wilt was unknown. Therefore, these traditional populations were subjected to the Fusarium wilt tolerance test. One hundred and sixty (160) two-months-old seedlings of the traditional accessions were distributed in 8 completely randomized blocks and inoculated by Fusarium oxysporum f. sp. elaeidis. The appearance of external symptoms on inoculated plantlets was observed over the duration of 5 months, and internal symptoms remarked after plantlets dissection. Index of the Fusarium wilt susceptibility of each progeny was determined. A third of traditional genotypes tested (Dompleu Kp 03, Gbangbegouiné Doua 01, Gbangbegouiné Kla 01 and Gbatonguin Yod 02) proved to be highly tolerant to the wilt disease. Four traditional accessions (Bogouiné Sad 02, Dompleu Kp 01, Dimgouin Zoh 02 and Blolé Dio 05) showed low tolerance, while the last four traditional genotypes (Blolé Oul 03, Koutongouiné Iba 02, Blolé Dio 02 and Dompleu Dou 03) were sensitive to the wilt disease.
油棕(Elaeis guineensis Jacq.)的遗传改良是基于重复的互惠选择方案，涉及两组生产成分互补的种群。A组主要来自亚洲，而B组包括非洲的油棕种群。为了增加遗传变异和丰富这一B组的农艺品质，在Côte科特迪瓦西部进行了一次调查。在Man地区收集到12种传统基因型。众所周知，它们能生产一种相当流动的棕榈油，但它们对枯萎病的作用尚不清楚。因此，对这些传统种群进行了枯萎病耐受性试验。将160株2个月龄的传统种质幼苗分成8个完全随机分组，用尖孢镰刀菌接种。在5个月的时间内观察接种植株的外部症状的出现，并在植株解剖后出现内部症状。测定了各子代对枯萎病的敏感性指数。三分之一的传统基因型(Dompleu Kp 03、gbangbegouin Doua 01、gbangbegouin Kla 01和Gbatonguin Yod 02)被证明对枯萎病具有高度耐受性。4个传统基因型(bogouin Sad 02、Dompleu Kp 01、Dimgouin Zoh 02和bloleedio 05)对黄萎病的耐受性较低，其余4个传统基因型(blooleoul 03、koutongouine Iba 02、blooledio 02和Dompleu Dou 03)对黄萎病敏感。
{"title":"Assessment of Tolerance to Fusarium Wilt of some Traditional Accessions of Oil Palm (Alaeis guineensis Jacq.) Collected in Man, West of Cote d'Ivoire","authors":"Kablan Kan Aurore Bel Martine, S. Diabaté, K. J. Noel, K. Hilaire, Koné Mongomaké","doi":"10.33687/phytopath.007.02.2593","DOIUrl":"https://doi.org/10.33687/phytopath.007.02.2593","url":null,"abstract":"The oil palm (Elaeis guineensis Jacq.) genetic improvement is based on a recurrent reciprocal selection scheme, involving two groups of populations whose production components are complementary. Group A is essentially coming from Asia, while group B includes African oil palm populations. In order to increase genetic variability and to enrich the agronomic qualities of this group B, a survey was carried out in Western Côte d'Ivoire. Twelve traditional genotypes were collected in the Man area. They are known to produce a rather fluid palm oil but their behavior against Fusarium wilt was unknown. Therefore, these traditional populations were subjected to the Fusarium wilt tolerance test. One hundred and sixty (160) two-months-old seedlings of the traditional accessions were distributed in 8 completely randomized blocks and inoculated by Fusarium oxysporum f. sp. elaeidis. The appearance of external symptoms on inoculated plantlets was observed over the duration of 5 months, and internal symptoms remarked after plantlets dissection. Index of the Fusarium wilt susceptibility of each progeny was determined. A third of traditional genotypes tested (Dompleu Kp 03, Gbangbegouiné Doua 01, Gbangbegouiné Kla 01 and Gbatonguin Yod 02) proved to be highly tolerant to the wilt disease. Four traditional accessions (Bogouiné Sad 02, Dompleu Kp 01, Dimgouin Zoh 02 and Blolé Dio 05) showed low tolerance, while the last four traditional genotypes (Blolé Oul 03, Koutongouiné Iba 02, Blolé Dio 02 and Dompleu Dou 03) were sensitive to the wilt disease.","PeriodicalId":36106,"journal":{"name":"International Journal of Phytopathology","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2018-06-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"77891990","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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