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Antimycotic Potential Assessment of Trichoderma Species and Fungicides for Sustainable Management of Sclerotinia trifoliorum causing Stem and Crown Rot of Trifolium alexandrinum L. 木霉菌种抑菌潜力评价及杀菌剂对亚历山大三叶草茎腐病和冠腐病的可持续治理
Q4 Agricultural and Biological Sciences Pub Date : 2022-08-30 DOI: 10.33687/phytopath.011.02.4271
Anjum Faraz, I. Haq, S. Ijaz, S. T. Sahi, Imran H. Khan
Sclerotinia trifoliorum, the fungal plant pathogen first reported in 2021 from Pakistan on Trifolium alexandrinum L. (Egyptian clover; an annual winter fodder crop), causing Stem and crown rot disease. About 46% to 55% incidence of this disease was recorded on E. clover cultivated in the irrigated tract of the country in 2018-19. This disease is subjecting significant crop losses and drastically reducing growth. An integrated disease management approach employing biological and chemical control was adopted to manage this wide-spreading fungal pathogen. The fungal antagonists, including Trichoderma harzianum, T. longibrachiatum, and T. asperellum Moreover, fungicides, including Thiophanate Methyl, Tebuconazole, Tubeconazole+Emdachloprid, Chlorothalonil+cymoxanil, Azoxystrobin, Pyraclostrobin+Metiram, and Mancozeb+Metalaxyl were tested under in vitro and field conditions. Among Trichoderma species, the best response was achieved by T. harzianum with 80.61% inhibition compared to control. Among concentrations of T. harzianum, the best response was achieved on 1/10 (1.24 cm) with 2.4 average No. of Sclerotia and 66% inhibition. Under filed condition experiments, the data regarding Disease severity in T. harzianum treated trays was 51.7% compared to untreated/control 73.5%. Besides disease control, the application of T. harzianum showed a significant increase in green and dry fodder weight (851 grams with 87 grams of dry weight) than untreated/control (561 grams with 55 grams of dry weight) in the fourth cut. For chemical evaluation, seven fungicides tested at three concentrations under in vitro trials among these Thiophanate methyl (0.5 cm) with 90.7% inhibition were found more effective. Thiophanate Methyl's application significantly reduced the disease severity compared to control plants with disease severity in fungicide-treated trays was 28.7% compared to untreated/control 73.5% and significant increase green and dry fodder weight (931 grams with 92 grams of dry weight) than untreated/control (561 grams with 55 grams of dry weight) in the fourth cut.
2021年首次报道的巴基斯坦三叶草(Trifolium alexandrinum L.)真菌植物病原体三叶菌核病(Sclerotinia trifoliorum);一种一年生冬季饲料作物),引起茎腐病和冠腐病。2018- 2019年,在全国灌溉区种植的三叶草中,该病的发病率约为46%至55%。这种疾病使作物遭受重大损失,并大大减少生长。采用生物防治和化学防治相结合的综合疾病管理方法来控制这种广泛传播的真菌病原体。对哈兹木霉、长尾曲霉、曲霉等真菌拮抗剂进行了体外和田间试验,并对噻吩酸甲酯、戊唑唑、戊唑唑+氨氯啉、百菌清+cymoxanil、氮嘧菌酯、吡唑菌酯+美替兰、代森锌+甲氨酯等杀菌剂进行了体外和田间试验。在木霉中,哈茨木霉的抑菌效果最好,抑菌率为80.61%。在不同浓度下,1/10 (1.24 cm)的响应最佳,平均响应数为2.4。菌核抑制66%。在田间条件试验中,处理过哈氏霉菌托盘的疾病严重程度为51.7%,而未处理/对照为73.5%。除防病外,施用哈兹兰在第4个节育时青饲料重(851克,干重87克)显著高于未施用/对照(561克,干重55克)。化学评价方面,7种杀菌剂在三种浓度下的体外试验中,甲基硫代盐(0.5 cm)的抑菌率为90.7%。与对照植株相比,施用甲基硫代盐显著降低了植株的疾病严重程度,其中杀菌剂处理的托盘的疾病严重程度为28.7%,而未处理/对照为73.5%,并且在第四节显著增加了青饲料和干料重(931克,干重92克),比未处理/对照(561克,干重55克)。
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引用次数: 1
Detection of Resistance against Leaf Spot, Yellow Mosaic and Urdbean Leaf Crinkle Viruses in Mash Bean (Vigna mungo) 豆粕对叶斑病、黄花叶病和豆皱病的抗性检测
Q4 Agricultural and Biological Sciences Pub Date : 2022-08-30 DOI: 10.33687/phytopath.011.02.4274
M. Hussain, Amir Afzal, M. Zeeshan, Sairah Syed, T. Hussain, G. Nabi, B. Ahmad, M. Ijaz
Among foliar diseases Leaf spot, yellow mosaic virus (YMV) and Urd bean leaf crinkle virus (ULCV) are destructive diseases of mash bean (Vigna mungo) in Pakistan. Incorporation of resistance against biotic stress in plants is traditional strategy of crop improvement through breeding. Effective breeding for biotic stresses entails readily accessible resistant germplasm. 67 genotypes were evaluated for multi disease resistance was tested for two consecutive years (2019 and 2020) at Barani Agricultural Research Institute, Chakwal. Experiment was repeated with an idea to decrease the effect of environment and those material was selected which performed same in both years. Out of 67 genotypes 12 were resistant against leaf spot infection and 19 genotypes were found highly resistant against yellow mosaic virus during both years. In case of ULCV disease did not appear during 2020. However, 19 genotypes showed resistance against ULCV during first year of experiment. Two genotypes namely 3CM-703 and 4CM-720 exhibited resistance response to all foliar diseases under study. Three genotypes viz. 4CM-716, 4CM-720, 5CM-708 demonstrated resistance against YMV as well as leaf spot. 4CM716, 4CM720, 5CM708, 7CM701, 7CM702, 7CM704, 7CM705, 7CM706 expressed resistance against leaf spot and ULCV simultaneously. The data generated is useful for improvement of mash bean through breeding.
在叶面病害中,叶斑病、黄花叶病毒(YMV)和乌豆叶皱病毒(ULCV)是巴基斯坦土豆泥(Vigna mungo)的破坏性病害。在植物中加入抗生物胁迫能力是作物育种改良的传统策略。对生物胁迫的有效育种需要易于获得的抗性种质。在Chakwal巴拉尼农业研究所连续两年(2019年和2020年)对67个基因型进行了多病抗性评估。本着减少环境影响的思想,反复试验,选择了两年内性能相同的材料。在67个基因型中,12个基因型对叶斑病具有抗性,19个基因型对黄花叶病毒具有高度抗性。在2020年期间没有出现ULCV疾病。然而,19个基因型在第一年的试验中表现出对ULCV的抗性。2个基因型3CM-703和4CM-720对所研究的所有叶面病害均表现出抗性反应。4CM-716、4CM-720、5CM-708三个基因型对YMV和叶斑病均有抗性。4CM716、4CM720、5ccm708、7CM701、7CM702、7CM704、7CM705、7CM706同时表达对叶斑病和ULCV的抗性。所得数据对豆荚的育种改良有一定的参考价值。
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引用次数: 0
Flower Induction and Control of Inflorescence Diseases in Mangoes using selected Fungicides and Chemical Fertilizers 杀菌剂和化肥对芒果花序病害的诱导和防治作用
Q4 Agricultural and Biological Sciences Pub Date : 2022-04-30 DOI: 10.33687/phytopath.011.01.3555
Ahmad R. Alvi, S. Chohan, M. Abid, M. T. Malik, H. Riaz
Mango (Mangifera indica L.) is widely considered an important fruit and a major crop in tropical and subtropical regions around the world. Mango is susceptible to a variety of diseases throughout its life cycle, but diseases that strike during the flowering stage cause significant losses and have a direct impact on productivity. Flowering in mango is unreliable from season to season because environmental signals for flower initiation are frequently inconsistent. During the current research, we identify the most promising laboratory-grade synthetic chemical, chemical fungicide, or their feasible combination that promotes floral development, induces flowering, and combats inflorescence diseases, which reduce production. For this investigation, a field trial was conducted with multiple treatments. Compared to the control, the potassium nitrate treatment resulted in the highest flowering/flower emergence (8.67%), followed by calcium carbonate (7.33%) and potassium nitrate with the combination of Champion (6.66%) and Cabrio Top (5.32). Minimum flowering occurred on plants treated with fungicides alone, i.e., Contaf Plus (3.33%), compared to the control (1.33%). Contaf Plus was the most effective chemical against all inflorescence diseases in the field. The disease with the highest incidence was blossom blight at 42.90%, followed by apical necrosis at 39%, mango deformity at 17.70%, and powdery mildew at a low of 0.40%. The combined application of potassium nitrate and Copper hydroxide exhibited the best response in the field for inducing flowering and flower set on mango inflorescences, while potassium nitrate and calcium nitrate were the most effective for inducing flowers on mango trees when treated independently. Current research is extensive on the inducement of flowering and inhibition of flower disease through the application of chemicals alone or in combination.
芒果(Mangifera indica L.)被广泛认为是世界热带和亚热带地区的重要水果和主要作物。芒果在整个生命周期中易受各种疾病的影响,但在开花阶段发生的疾病会造成重大损失,并直接影响生产力。不同季节的芒果开花是不可靠的,因为开花的环境信号经常不一致。在目前的研究中,我们确定了最有前途的实验室级合成化学品,化学杀菌剂,或它们的可行组合,促进花的发育,诱导开花,对抗花序疾病,减少产量。在这项研究中,进行了多种处理的现场试验。与对照相比,硝酸钾处理的开花/出花率最高(8.67%),其次是碳酸钙处理(7.33%)和硝酸钾与冠军(6.66%)和卡布里奥(5.32%)组合处理。与对照(1.33%)相比,单独使用杀菌剂(Contaf Plus)处理的植株开花最少(3.33%)。在田间试验中,对所有花序病害的防治效果最好。发病率最高的是花枯病(42.90%),其次是根尖坏死(39%)、芒果畸形(17.70%)和白粉病(0.40%)。田间施用硝酸钾和氢氧化铜对芒果树的诱导开花和坐花效果最好,单独施用硝酸钾和硝酸钙对芒果树的诱导花效果最好。目前,通过单独或联合应用化学物质来诱导开花和抑制花病的研究非常广泛。
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引用次数: 0
Identification of Durable Resistance against Yellow Rust 黄锈病持久抗性鉴定
Q4 Agricultural and Biological Sciences Pub Date : 2022-04-30 DOI: 10.33687/phytopath.011.01.4079
Amir Afzal, A. Riaz, Sharmin Ashraf, Javed Iqbal, M. Ijaz, F. Naz, Syed K. N. Shah
Yellow (stripe) rust of wheat is responsible for a severe decrease in crop yield worldwide. Deployment of durable disease resistant cultivars is the best strategy being cost effective and safe. A comprehensive study was conducted to identify sources of durable resistance against stripe rust of wheat. Initially, surveillance of the crop was conducted to expose the status of the dilemma. The study discovered that disease is more prevalent in the region existing in Khyber Pakhtunkhwa (KPK) and the Northern Region of Punjab. Race identification of the pathogen under glasshouse conditions revealed high diversity and at least eleven races were designated. Prevalence of the race 70E0 was most dominating (39 %). Cultivation of multi-location trap nurseries yielded valuable information demonstrating disease-fighting genes where, at all locations, Yr5, Yr10, Yr15, and YrSp were effective. Seventy-two advanced lines collected from research institutions were screened in a glasshouse to categorize the test material based on their response to disease at the seedling stage. Test material comprising seventy-two advanced lines collected from different research institutions was screened in a glasshouse to categorize the test material on the ground of their response against disease at the seedling stage. Slow and fast rusters were categorized by studying susceptible and moderately susceptible seedlings in the field for two years. Genetic diversity in the host allows changes in the genetic organization to adapt to environmental changes. Coefficient parentage revealed the test material's restricted genetic base. In the pedigree of wheat advanced lines, Pastor, Kauz, Inqilab-91, Sokoll, Ae. Aquarosa (211), WBLL-1, Kukuna, and Millan were 60% out of a total of 72 parents. Findings of the present studies revealed persistent resistant genotypes with a broad genetic background are needed to feed a growing population.
小麦黄(条)锈病是全球农作物产量严重下降的原因。选用耐久的抗病品种是经济有效且安全的最佳策略。对小麦条锈病持久抗性来源进行了综合研究。最初,对作物的监测是为了揭露这种困境的现状。该研究发现,该病在开伯尔-普赫图赫瓦省(KPK)和旁遮普北部地区更为普遍。温室条件下病原菌的小种鉴定显示出较高的多样性,至少确定了11个小种。70E0的流行率最高(39%)。多地点诱捕苗圃的培养获得了证明抗病基因的宝贵信息,其中Yr5、Yr10、Yr15和YrSp在所有地点都是有效的。从研究机构收集的72个高级品系在温室中进行筛选,根据其苗期对病害的反应对试验材料进行分类。试验材料包括从不同研究机构收集的72个高级品系,在一个温室中筛选,根据它们在苗期对疾病的反应对试验材料进行分类。通过2年田间易感和中易感幼苗的研究,对慢锈病和快锈病进行了分类。宿主的遗传多样性允许遗传组织的变化来适应环境的变化。系数亲子鉴定揭示了受试材料有限的遗传基础。在小麦先进系系谱中,Pastor, Kauz, Inqilab-91, Sokoll, Ae。Aquarosa(211)、WBLL-1、Kukuna和Millan在72对父母中占60%。目前的研究结果表明,需要具有广泛遗传背景的持久耐药基因型来养活不断增长的人口。
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引用次数: 3
Evaluation of Integrated Management Approaches against Citrus Nematode (Tylenchulus semipenetrans) in Pakistan 巴基斯坦柑桔线虫综合防治方法评价
Q4 Agricultural and Biological Sciences Pub Date : 2022-04-30 DOI: 10.33687/phytopath.011.01.4092
W. Khan, N. Javed, M. Naveed, S. Khan, S. Ahmad
Present study was conducted to explore the nematicidal potential of different synthetic chemicals, biopesticides and antagonists against citrus decline. Effect of eleven chemicals, seven biopesticides and two antagonists on juvenile mortality was evaluated in vitro. Four concentrations (2S, S, S/2 and S/4) of each chemical were prepared on the basis of recommended dose for each chemical. Juvenile mortality of citrus nematodes was calculated after 24, 48 and 72 hour of exposure to chemicals. Rugby expressed maximum percentage of juvenile mortality at 2S, S, S/2 and S/4 concentration. Mortality percentage observed by Rugby, Furadan, Match and Cartap after 72 h at 2S concentration was (100, 100, 95.44, 88.23%) whereas at S/4 concentration mortality percentage was (76.32, 81.18, 62.15, 55.28%) respectively. Among biopesticides maximum percentage mortality observed by Proclaim and Cure after 72 hours at 2S concentration was (83.87, 80.44%) while at S/4 concentration it was (59.87, 57.38%) respectively. Cultural filtrates of two antagonist Trichoderma harzianum and Trichoderma viridi were evaluated at S, S/2 and S/4 concentration under lab conditions. Maximum mortality (88.42%) was observed when both antagonists were applied in combined treatment at S concentration after 72 hours of exposure. Two best performing chemicals, one biopesticide and one antagonist were evaluated under greenhouse against T. semipenetrans on Citrus jambhiri Lush (rough lemon) and their effect on plant growth and nutrient uptake was measured. Nitrogen uptake was measured by following micro Kjeldahl method. For phosphorus and potassium uptake absorbance of samples at 720 nm was measured with the help of spectrophotometer. Phosphorus was calculated by comparing standard curve already prepared while potassium uptake was assessed by flame photometer method after digestion. All the treatments were found significantly effective against citrus nematode but maximum plant height, stem diameter, number of leaves, root length, root weight, shoot weight, and number of feeder roots (90 cm, 2.4 cm, 102, 48.3 g, 12.1 g, 48.8 g and 70) as compared to control when Rugby, Furadan, Proclaim and T. harzianum were applied in combination. Similarly maximum uptake of Nitrogen (3.14) Phosphorous (2.44%) and Potassium (1.95%) was observed when all the treatments were applied in combination. The results of present study will be helpful in selecting the suitable chemicals for growers having problems of citrus nematodes in orchards.
研究了不同合成化学药剂、生物农药和拮抗剂对柑桔枯萎病的杀线虫作用。研究了11种化学药剂、7种生物农药和2种拮抗剂对幼体死亡率的影响。在推荐剂量的基础上制备了4种浓度(2S、S、S/2和S/4)。分别计算柑桔线虫暴露于化学物质24、48和72小时后的幼虫死亡率。拉格比表达了2S、S、S/2和S/4浓度下幼鱼死亡率的最大值。2S浓度下72h, Rugby、Furadan、Match、Cartap的死亡率分别为(100、100、95.44、88.23%),S/4浓度下72h的死亡率分别为(76.32、81.18、62.15、55.28%)。2S浓度下的最大死亡率为83.87、80.44%,S/4浓度下的最大死亡率为59.87、57.38%。在实验室条件下对两种拮抗剂哈茨木霉和病毒木霉的培养滤液进行了S、S/2和S/4浓度的评价。两种拮抗剂以S浓度联合使用,暴露72小时后死亡率最高(88.42%)。在温室条件下,评价了生物农药和拮抗剂两种药剂对粗柠檬上半透虫的防治效果,并测定了它们对粗柠檬植株生长和养分吸收的影响。采用微量凯氏定氮法测定氮吸收。用分光光度计测定了样品在720 nm处的磷、钾吸收吸光度。通过比较已制备的标准曲线计算磷,消化后用火焰光度计法测定钾的吸收。拉格比、弗拉丹、宣布和哈兹纳姆组合施用对柑橘线虫的最大株高、茎粗、叶数、根长、根重、茎重和食根数(90 cm、2.4 cm、102、48.3 g、12.1 g、48.8 g和70)均显著高于对照。所有处理组合施用时,氮(3.14%)、磷(2.44%)和钾(1.95%)的吸收量最大。本研究结果将有助于柑橘线虫防治问题的种植者选择合适的药剂。
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引用次数: 1
Qualities of Soybean Grains Harvested on Crops Treated with Neem Seeds Extract in Field Conditions against Asian Rust 在田间条件下用印楝籽提取物处理的大豆籽粒抗亚洲锈病品质研究
Q4 Agricultural and Biological Sciences Pub Date : 2022-04-30 DOI: 10.33687/phytopath.011.01.4044
N. P. Angèle, Soupi N. M. Solange, Gbaporo G. F. Christian, Mvondo N. Dorotée, A. Zachée
In soybean production, qualities of grains harvested are usually affected according to the development of diseases and control methods used in the field. This study aims to evaluate the impact of neem seeds extract (AENS) on soybean grains health after crops treatment in field conditions for the control against Asian rust. Experiment was conducted in Mimetala and Nkometou, located in the agroecological zone V of Cameroun. Three varieties of soybean (“Locale”, TGX 1835-10E and TGX1910-14E) were used in a completely randomized blocks design containing five treatments: T0 (control), T1, T2, T3 (25, 50, 100 g/L of extract respectively) and T4 (5.33 g/L of Plantineb 80wp). The disease was identified on the harvested grains; its incidence on those grains was evaluated and some biochemical parameters were assessed by extraction and determination of their contents. Many grains were attacked by Asian rust. The highest incidence was obtained in the seeds from untreated plots (35 %), compared to those from T1, T2, T3 (1.14; 1.07 and 0.95 % respectively). The different doses of AENS improved the content of all biochemical compounds tested in harvested grains compared with the T0 and T4 treatments. TGX1910-14E variety presented the highest content. In T3,grain contents in biochemical compounds were 415.17; 118.83; 118.33; 44.43; 36.53 and 10.83 mg/g of dry matter from total proteins, globulin, albumin, Glutelin, prolamin and glucide respectively. Meanwhile, in T4 treatments, grain contents were 403.49; 112.95; 112.78; 40.58; 33.90 and 9.91 mg/g of dry matter from the same compounds respectively. This study shows that the application of neem seeds extract on soybean crops for the control of Asian rust in field conditions at the maximum dose (e.g. 100 g/L) can improve the qualities of harvested grains. The neem extract as a natural substance should be used in integrated pest management of soybean and other crops.
在大豆生产中,由于病害的发展和田间控制方法的不同,通常会影响收获的籽粒品质。本研究旨在评价印楝种子提取物(AENS)在田间防治亚洲锈病条件下对作物处理后大豆籽粒健康的影响。试验在喀麦隆第五农业生态区的米梅塔和恩科梅托进行。以“Locale”、TGX 1835-10E和TGX1910-14E 3个大豆品种为试验材料,采用完全随机区组设计,共设5个处理:T0(对照)、T1、T2、T3(分别添加25、50、100 g/L提取物)和T4(添加5.33 g/L大车前碱80wp)。在收获的谷物上发现了这种疾病;通过对其含量的提取和测定,评价了其在这些谷物中的发病率和一些生化参数。许多谷物受到亚洲铁锈的侵袭。与T1、T2、T3处理的种子相比,未经处理的种子发病率最高(35%)(1.14;分别为1.07和0.95%)。与T0和T4处理相比,不同剂量的AENS处理提高了收获籽粒中所有生化化合物的含量。TGX1910-14E品种含量最高。T3期,籽粒生化化合物含量为415.17;118.83;118.33;44.43;总蛋白、球蛋白、白蛋白、谷蛋白、蛋白胨和葡萄糖的干物质含量分别为36.53和10.83 mg/g。T4处理,籽粒含量为403.49;112.95;112.78;40.58;分别为33.90和9.91 mg/g。本研究表明,在大田条件下施用印楝种子提取物防治亚洲锈病,最大剂量(如100 g/L)可改善收获的籽粒品质。楝树提取物作为一种天然物质,可用于大豆和其他作物病虫害的综合治理。
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引用次数: 0
Late Blight of Potato (Phytophthora Infestans) Management through Botanical Aqueous Extracts in Temperate Climatic Conditions 温带气候条件下植物水提取物对马铃薯晚疫病的防治
Q4 Agricultural and Biological Sciences Pub Date : 2022-04-30 DOI: 10.33687/phytopath.011.01.4131
Basharat Mehmood, A. Azad, Nasir Rahim, Shameem Arif, M. R. Khan, Abid Hussain, Muhammad Tariq-Khan, Muhammad T. Younis, A. Bashir, Sohail Ahmed, J. R. Khan, Sahira Tariq, Muhammad Jamil
Phytophthora infestans causing late blight is one of the most devastating tomato and potato disease that caused considerable yield losses globally including Pakistan. Due to repeated and injudicious synthetic fungicides applications for the control of late blight of potato, the fungicide resistance in P. infestans led to persistence and surveillance late blight of potato. The study was carried out to evaluate the efficacy of Garlic (Allium sativum), Neem (Azadirachta indica), Turmeric (Curcuma longa), Mint (Mentha) at 10%, 20% and 30% concentration as bio-fungicides against late blight of potato. In-vitro effect of plant extracts of A. sativum, A. indica, C. longa and Mentha were evaluated on percent inhibition and radial growth of pathogen. A. sativum and A. indica at 30% concentration was found more effective in minimizing the mycelial growth of P. infestans with inhibition of 58.4% and 43.9% respectively as compared to control. In the greenhouse trial, overall potato late blight disease incidence was minimum 5.81% where A. sativum extract was  used followed by A. indica at 30% concentration resulted 8.45% incidence as compared to control 61.18%. Furthermore, the 30% aqueous extracts of A. sativum  found highly effective against late blight disease with 15.4% severity,  as compared to control with 54.13% disease coverage. Application of A. sativum and A. indica aqueous plant extracts with a concentration of 30% was found most promising and effective measure against late blight pathogen. Study provides environmentally benign mileage in organic vegetable production against hazardous synthetic agro-chemicals.
引起晚疫病的疫霉是最具破坏性的番茄和马铃薯病害之一,在包括巴基斯坦在内的全球造成了相当大的产量损失。由于合成杀菌剂在马铃薯晚疫病防治中的反复应用和不合理,导致马铃薯晚疫病的持续发生和监测。研究了大蒜(Allium sativum)、印楝(Azadirachta indica)、姜黄(Curcuma longa)、薄荷(Mentha)在10%、20%和30%浓度下对马铃薯晚疫病的生物杀菌剂效果。研究了苜蓿、籼米、龙葵和薄荷提取物对病原菌的体外抑制率和径向生长的影响。与对照相比,30%浓度的苜蓿和籼稻对病原菌菌丝生长的抑制率分别为58.4%和43.9%。在大棚试验中,马铃薯晚疫病总发病率最低,以苜蓿提取物最低,为5.81%,其次是30%浓度的籼稻,发病率为8.45%,而对照为61.18%。此外,与疾病覆盖率为54.13%的对照相比,30%的苜蓿水提取物对晚疫病的防治效果非常好,严重程度为15.4%。应用浓度为30%的sativum和a . indica植物水提液防治晚疫病是最有希望和有效的措施。研究为有机蔬菜生产对抗有害的合成农用化学品提供了环保的途径。
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引用次数: 1
Preliminary Selection and Evaluation of Fungicides and Natural Compounds to Control Grey Mold Disease of Rose Caused by Botrytis cinerea 防治玫瑰灰霉病杀菌剂及天然化合物的初步选择与评价
Q4 Agricultural and Biological Sciences Pub Date : 2022-04-30 DOI: 10.33687/phytopath.011.01.4181
G. H. Jatoi, M. Abro, S. M. Ahmed, L. Al-Ani, Umed Ali, M. A. Jatoi, Isabel M. G. Figari, Jahansher Qambrani, I. Ahmed, A. S. Soomro, N. K. Khaskheli
Botrytis cinerea is a plant fungal pathogen causing the grey mold disease of rose ‎‎(Rosa indica L.). Finding new and alternative environment-friendly control strategies than ‎hazardous chemicals on different crop diseases is a crucial and healthy step to cope with ‎the current challenges of climate change. ‎ Therefore, this study aimed to evaluate the efficacy of different botanical extracts and biocontrol agents (biopesticides) along with different fungicides against B. cinerea under in-vitro conditions. Three different concentrations i.e., 100, 200, and 300 ppm of five fungicides namely Acrobate, Melody, Cabrio top, Antracol, and Copper oxychloride, botanical extracts of eight plants Dhatura, Ginger, Aak, Neem and Onion, at three different doses of 5, 10, and 15%, and eleven biocontrol fungal agents were used as antagonistic under in-vitro on rose plants, The survey of ‎disease incidence% of grey mold on the rose crop in the region shows that the Hyderabad region has a maximum (60%) disease incidence as compared to Tandojam region ‎‎(40%). Among fungicides, the Cabrio top significantly reduced linear colony growth (31 mm) of B. cinerea at 300 ppm concentration. Among botanicals, extract of neem plant exhibited significantly lowest colony growth (23.33 mm) followed by the ginger plant (25 mm) and dhatura plant (26 mm). The higher concentration of fungicides and higher doses (15%) of botanicals extracts appeared significantly efficient to control the pathogen B. cinerea. Among biopesticides, Fusarium solani appeared prominent in reducing colony growth (25.16 mm) of the pathogen but the difference was not significant 300 with most of the tested biocontrol agents. The recommendation in this study is the high ability of botanical extracts and biocontrol agents in reducing the growth of grey mold, potentially considering using them instead of synthetic fungicides and more safety for the ecosystem. Keywords: Trichoderma, Fusarium, dhatura, botanical, biological control, synthetic fungicides.
灰霉菌(Botrytis cinerea)是一种引起玫瑰灰霉病的植物真菌病原体。针对不同作物病害,寻找新的、替代有害化学品的环境友好型控制战略,是应对当前气候变化挑战的关键和健康的一步。因此,本研究旨在评价不同植物提取物和生物防治剂(生物农药)以及不同杀菌剂在体外条件下对灰霉病芽孢杆菌的药效。以百、200、300ppm 5种杀菌剂(acromatate、Melody、Cabrio top、Antracol、Copper oxychloride)和八种植物(Dhatura、Ginger、Aak、楝树、Onion)提取物(5、10、15% 3种剂量)和11种生物防真菌剂对玫瑰植株进行体外拮抗。对该地区玫瑰作物灰霉病发病率的调查显示,与坦多贾姆地区(40%)相比,海德拉巴地区的发病率最高(60%)。在杀菌剂中,在300 ppm浓度下,Cabrio顶部显著降低了灰绿杆菌的线性菌落生长(31 mm)。其中,印楝提取物集落生长最低(23.33 mm),其次是生姜(25 mm)和土地黄(26 mm)。较高浓度的杀菌剂和较高剂量(15%)的植物提取物对葡萄球菌的防治效果显著。在生物农药中,番茄镰刀菌对病原菌菌落生长(25.16 mm)有显著的抑制作用,但与大多数生物防治剂差异不显著。本研究推荐植物提取物和生物防治剂对灰霉病的抑制能力强,可以考虑使用它们代替合成杀菌剂,对生态系统更安全。关键词:木霉,镰刀菌,赤眼菌,植物,生物防治,合成杀菌剂
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引用次数: 0
Exploration of Resistance and Susceptibility in Chilli Varieties/Advanced Lines against Fusarium Wilt Caused by Fusarium oxysporum f. sp. capsici (FOC) 辣椒品种/高级品系对辣椒枯萎病(FOC)的抗性和敏感性探讨
Q4 Agricultural and Biological Sciences Pub Date : 2022-04-30 DOI: 10.33687/phytopath.011.01.4148
S. Saeed, S. T. Sahi, M. Atiq, Muhammad Shahid, Muhammad Arshad
Chilli (capsicum annum L.) is an important edible spice crop grown in tropical areas of world due to its richness of nutrients like carotinoides, fibers, mineral components, oils, proteins and vitamins. A number of biotic and abiotic factors are challenging devastatingly the successful production of chilli. Among all of these factors, Fusarium wilt caused by Fusarium oxysporum f.sp.capsici (Foc) is a potential risk of declining its yield every year. Among all management approaches, use of resistant varieties is the best option towards Foc. For this purpose in contemporary study twenty five varieties/advanced lines of chilli were evaluated against Fusarium wilt under natural field conditions in research area, Department of Plant Pathology, University of Agriculture Faisalabad for two years 2017-18 and 2018-19 under randomized complete block design (RCBD). Results exhibited that none of varieties/advanced line expressedimmune response against the disease. Only one variety (BPVLC 14-1) was resistant with 18.76% disease incidence. Uttal, fengaio, Glaxy-2, Big daddy, GHHP 01, PH-275, Super sky AB, HPO33 and Super king were found moderately resistant (MR) with 21-40% disease incidence. Four varieties/advanced lines Hot-701. Hot shot, Omega and Silkey Red showed moderately susceptible response (MS) with 41-50% and Four (Super hot, Patyala F1, Angel F1 and Green king) were susceptible (S) with 51-70% incidence of Fusarium wilt. Seven (Tejal, BSS-410, Big Red AB, SB 6864-HM, Glory F1, Revival and Amber F1) varieties / advanced lines exhibited highly susceptible (HS) response.
辣椒(capsicum annum L.)富含类胡萝卜素、纤维、矿物成分、油脂、蛋白质和维生素等营养成分,是世界热带地区重要的食用香料作物。许多生物和非生物因素对辣椒的成功生产构成了毁灭性的挑战。其中,由辣椒枯萎病(Fusarium oxysporum f.sp.capsici, Foc)引起的枯萎病(Fusarium oxysporum f.sp.capsici)是其产量逐年下降的潜在风险。在所有管理方法中,使用抗性品种是防治Foc的最佳选择。为此,本研究采用随机完全区设计(RCBD),于2017-18年和2018-19年在费萨拉巴德农业大学植物病理学系研究区域的自然田间条件下,对25个辣椒品种/高级品系的枯萎病抗性进行了评估。结果表明,所有品种/先进系均未表现出抗病性。只有一个品种(BPVLC 14-1)具有抗性,发病率为18.76%。乌达尔、丰爱、Glaxy-2、Big daddy、GHHP 01、PH-275、Super sky AB、HPO33和Super king为中等抗性(MR),发病率为21-40%。四个品种/先进系Hot-701。Hot shot、Omega和Silkey Red表现为中度敏感反应(MS),发生率为41% ~ 50%;4个(superhot、Patyala F1、Angel F1和Green king)表现为51 ~ 70%的敏感反应(S)。7个品种/高级系(Tejal、BSS-410、Big Red AB、SB 6864-HM、Glory F1、Revival和Amber F1)表现出高敏感(HS)反应。
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引用次数: 0
Identification of Host Resistance against Bacterial Canker Disease on Apricot, Plum and Peach Grown in Punjab and KPK 旁遮普和KPK地区杏、李、桃寄主对细菌性溃疡病的抗性鉴定
Q4 Agricultural and Biological Sciences Pub Date : 2022-04-30 DOI: 10.33687/phytopath.011.01.4130
S. Bibi, M. Inam-ul-Haq, A. Riaz, S. Malik
Bacterial canker disease caused by the pathogen Pseudomonas syringae pv. syrinagae (Pss) has been spread widely in Pakistan,  in Punjab and KPK provinces specially in strone fruits like apricot, Peach and Plum. During the 2014–2015 growing period, diseased samples were obtained from peach, plum and  apricot trees showing bacterial canker  symptoms  in Punjab and KPK provinces and 48 Pseudomonas syringae  isolates were obtained. The main aim of this research study was to find out host resistance in the cultivars of Apricot, Peach and Plum against bacterial canker caused by Pss in Peach, Plum and Apricot cultivars grown in Punjab and KPK province of Pakistan. In a pathogenicity test, three Pss isolates (PS3, PS9 and PS17) were found to be highly virulent in peach, plum and apricot  and were tested for cultivar resistance to Pss. Leaves and shoots of five varieties of Peach Early grand, Florida king, 4 ½, 5 ½, 6 ½, Four varieties of Plum Red beauty, Fazal-e- manani, Stanley, Producer and Two varieties of Apricot Chinese apricot and golden amber were foliar sprayed with a mixed culture of Pss having concentration of 10-8 cfu ml−1 . Sprayed cultivars were covered with plastic bags for 3 days for retaining moisture and kept in glass house and were keenly observed for appearance of  symptoms. 5 ½ of peach, Fazal-e-manani of plum and Chinese apricot of apricot were found resistant while 6 ½ of peach, Stanley of plum were found susceptible and Golden amber of apricot was found moderately susceptible to Pss. This is the first report of host resistance response of apricot, peach and plum cultivars grown in Pakistan to Pss.
由紫丁香假单胞菌引起的细菌性溃疡病。syrinagae (Pss)在巴基斯坦,旁遮普省和KPK省广泛传播,特别是在杏仁、桃子和李子等坚硬的水果中。2014-2015年生长期,从旁遮普省和KPK省出现细菌性溃疡病症状的桃树、梅树和杏树中采集了病样,分离出48株丁香假单胞菌。本研究的主要目的是在巴基斯坦旁遮普省和KPK省的桃、李、杏品种中发现寄主对Pss引起的细菌溃疡病的抗性。3株Pss分离株(PS3、PS9和PS17)在桃、李和杏中均表现出高毒力,并进行了品种抗性试验。用浓度为10-8 cfu ml−1的Pss混合培养液,对5个品种的桃早大、佛罗里达王、4½、5½、6½,4个品种的梅红美人、Fazal-e- manani、Stanley、Producer和2个品种的杏、中国杏和金琥珀进行叶面喷施。喷施后的品种用塑料袋包住,保温3天,置于玻璃棚内,敏锐地观察症状的出现。桃的5½、李的Fazal-e-manani和杏的Chinese apricot对Pss有抗性,桃的6½、李的Stanley有易感,杏的Golden amber有中度易感。这是首次报道在巴基斯坦种植的杏、桃和李品种对Pss的抗性反应。
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引用次数: 1
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International Journal of Phytopathology
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