T. Tallei, Siti Marfuah, A. Abas, Andi Abram, Nelsyani Pasappa, Putri Anggini, A. Soegoto, Fatima Wali, T. Emran
{"title":"Nata as a source of dietary fiber with numerous health benefits","authors":"T. Tallei, Siti Marfuah, A. Abas, Andi Abram, Nelsyani Pasappa, Putri Anggini, A. Soegoto, Fatima Wali, T. Emran","doi":"10.5455/jabet.2022.d107","DOIUrl":"https://doi.org/10.5455/jabet.2022.d107","url":null,"abstract":"","PeriodicalId":36275,"journal":{"name":"Journal of Advanced Biotechnology and Experimental Therapeutics","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70796090","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
The goal of study was determined that Klebsiella pneumoniae isolates possessed Toxin-Antitoxin II genes such as relE and relB, other than if there was a correlation between the expression of these two genes and antibiotics, and they may participate in increasing the resistance to antibiotics in K. pneumoniae. Fifty-seven urine samples were collected, from Baghdad s’ hospitals, diagnosed and identified by phenotype and biochemical tests, and confirmed with VITEK 2 compact system. Only fifteen isolates which were identified as K. pneumoniae. Antibiotic sensitivity was identified by using twelve antibiotics discs and K. pneumoniae were resisted in 100% to Ceftriaxone, Amoxicillin, Ticarcillin, Ticarcillin+Clavulanic acid, Ceftazidime, Tetracycline, while other antibiotics were showed less percent of resistant. Minimum inhibitory concentrations (MICs) of antibiotics detected by using macro tube dilution method to identify the antimicrobial activity for K. pneumoniae, the MIC of Gentamicin and Doxycycline antibiotics was 1024 Mg/ml, 512 Mg/ml respectively. By polymerase chain reaction detected genes of relB, relE with 115bp. and 136pb. respectively, then gene expression of relB, relE was conducted by using (RT-qPCR) technique with treated sub MIC concentration of (Gentamicin and Doxycycline) antibiotics. This study was found only ten isolates harbored the two genes as well as, value of folding was increased expression fold of relB gene, but in the same time relE gene was decreased its fold change according to control infB1 gene expression results. This means the bacterial cell tolerance antibiotics sub MIC concentrations by maintaining the number of bacteria under stress of antibiotics. Finally, these findings suggest the potential of relB to give K. pneumoniae resistant to antibiotics in their infections when it was being under stress of antibiotics by toxin-antitoxin II system to stay life.
{"title":"Effect of gentamicin and doxycycline on expression of relB and relE genes in Klebsiella pneumonia","authors":"E. Sweedan, Z. Shehab, M. Flayyih","doi":"10.5455/jabet.2022.d145","DOIUrl":"https://doi.org/10.5455/jabet.2022.d145","url":null,"abstract":"The goal of study was determined that Klebsiella pneumoniae isolates possessed Toxin-Antitoxin II genes such as relE and relB, other than if there was a correlation between the expression of these two genes and antibiotics, and they may participate in increasing the resistance to antibiotics in K. pneumoniae. Fifty-seven urine samples were collected, from Baghdad s’ hospitals, diagnosed and identified by phenotype and biochemical tests, and confirmed with VITEK 2 compact system. Only fifteen isolates which were identified as K. pneumoniae. Antibiotic sensitivity was identified by using twelve antibiotics discs and K. pneumoniae were resisted in 100% to Ceftriaxone, Amoxicillin, Ticarcillin, Ticarcillin+Clavulanic acid, Ceftazidime, Tetracycline, while other antibiotics were showed less percent of resistant. Minimum inhibitory concentrations (MICs) of antibiotics detected by using macro tube dilution method to identify the antimicrobial activity for K. pneumoniae, the MIC of Gentamicin and Doxycycline antibiotics was 1024 Mg/ml, 512 Mg/ml respectively. By polymerase chain reaction detected genes of relB, relE with 115bp. and 136pb. respectively, then gene expression of relB, relE was conducted by using (RT-qPCR) technique with treated sub MIC concentration of (Gentamicin and Doxycycline) antibiotics. This study was found only ten isolates harbored the two genes as well as, value of folding was increased expression fold of relB gene, but in the same time relE gene was decreased its fold change according to control infB1 gene expression results. This means the bacterial cell tolerance antibiotics sub MIC concentrations by maintaining the number of bacteria under stress of antibiotics. Finally, these findings suggest the potential of relB to give K. pneumoniae resistant to antibiotics in their infections when it was being under stress of antibiotics by toxin-antitoxin II system to stay life.","PeriodicalId":36275,"journal":{"name":"Journal of Advanced Biotechnology and Experimental Therapeutics","volume":"55 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70796755","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Utari Satiman, M. Tulung, J. Pelealu, C. Salaki, B. Kolondam, T. Tallei, T. Emran, A. Pinaria
{"title":"Morphology, Diversity and Phylogenetic Analysis of Spodoptera exigua (Lepidoptera: Noctuidae) in North Sulawesi by Employing Partial Mitochondrial Cytochrome Oxidase 1 Gene Sequences","authors":"Utari Satiman, M. Tulung, J. Pelealu, C. Salaki, B. Kolondam, T. Tallei, T. Emran, A. Pinaria","doi":"10.5455/jabet.2022.d103","DOIUrl":"https://doi.org/10.5455/jabet.2022.d103","url":null,"abstract":"","PeriodicalId":36275,"journal":{"name":"Journal of Advanced Biotechnology and Experimental Therapeutics","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70796001","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pangasius catfish (Pangasianodon hypophthalmus) is popular among fish farmers of Bangladesh due to its hardy characteristics, fast growth, and its ability to survive in high densities. Many consumers love to buy this fish due to its low market price and delicious flashy meat, especially as live condition. Bacterial outgrowth in transport water is frequent consequence including some enteric groups like Salmonella spp. and E. coli. The study attempted to know the occurrence of Salmonella spp. and E. coli in water used during live transportation of Pangasius catfish in Bangladesh. Water samples were collected from three different Pangasius catfish transportation channels at 2 hours intervals at on-board transportation vehicle. The collected waters were then plated onto SS and EMB agar plates and obtained 15, 20, and 17 suspected isolates from channel 1, channel 2 and channel 3, respectively. The isolates were confirmed through PCR techniques; Salmonella spp. was found only in Channel 1 while E. coli were found in all the three sampling channels. Among the suspected isolates, 13 were positive for E. coli in channel 1, while 16 in both channel 2 and channel 3. Among the suspected isolates, 86.54% was E. coli positive, 1.92% was Salmonella positive, and 11.54% isolates was not confirmed. The results indicated that the fishes were contaminated with Salmonella spp. and E. coli species either in the culture systems or during handling and live transportation.
{"title":"Isolation and identification of Salmonella spp. and Escherichia coli from water used during live transportation of Pangasius catfish, Pangasianodon hypophthalmus","authors":"A. Bhuiyan, Md Hossain, M. Uddin, Michaela Haider","doi":"10.5455/jabet.2022.d146","DOIUrl":"https://doi.org/10.5455/jabet.2022.d146","url":null,"abstract":"Pangasius catfish (Pangasianodon hypophthalmus) is popular among fish farmers of Bangladesh due to its hardy characteristics, fast growth, and its ability to survive in high densities. Many consumers love to buy this fish due to its low market price and delicious flashy meat, especially as live condition. Bacterial outgrowth in transport water is frequent consequence including some enteric groups like Salmonella spp. and E. coli. The study attempted to know the occurrence of Salmonella spp. and E. coli in water used during live transportation of Pangasius catfish in Bangladesh. Water samples were collected from three different Pangasius catfish transportation channels at 2 hours intervals at on-board transportation vehicle. The collected waters were then plated onto SS and EMB agar plates and obtained 15, 20, and 17 suspected isolates from channel 1, channel 2 and channel 3, respectively. The isolates were confirmed through PCR techniques; Salmonella spp. was found only in Channel 1 while E. coli were found in all the three sampling channels. Among the suspected isolates, 13 were positive for E. coli in channel 1, while 16 in both channel 2 and channel 3. Among the suspected isolates, 86.54% was E. coli positive, 1.92% was Salmonella positive, and 11.54% isolates was not confirmed. The results indicated that the fishes were contaminated with Salmonella spp. and E. coli species either in the culture systems or during handling and live transportation.","PeriodicalId":36275,"journal":{"name":"Journal of Advanced Biotechnology and Experimental Therapeutics","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70796362","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Aspergillus niger grows faster than Escherichia coli in eosin methylene blue media and deter their growth by reducing the pH of the media","authors":"M. Hossain, M. Ali","doi":"10.5455/jabet.2022.d110","DOIUrl":"https://doi.org/10.5455/jabet.2022.d110","url":null,"abstract":"","PeriodicalId":36275,"journal":{"name":"Journal of Advanced Biotechnology and Experimental Therapeutics","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70796333","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Myocardial necrosis caused by ischemia is called a myocardial infarction (MI). which interrupts coronary blood supply. When the oxygen supply to the heart is insufficient to meet metabolic demands, myocardial ischemia occurs. Atherosclerosis, which obstructs the coronary arteries, is the most common underlying cause of myocardial ischemia. The role of arginase-1 (ARG-1) and serum lipids in the pathogenesis of myocardial infarction is becoming clearer. This study aims to see if there is a link between ARG-1 activity and MI in the Iraqi population. Between the first of November 2021 and the first of February 2022, 90 people were separated into two groups: 45 patients with MI and 45 healthy controls. Human ARG-1 was measured in serum blood using the ELISA method. The serum lipid was measured using the spectrophotometry technique. The current investigation discovered a substantial (p=0.01) rise in ARG-1 concentration compared to control groups, as well as a significant difference in blood lipid content between patients and control groups (p<0.05). Finally, ARG-1 may have a role to play role in the pathogenesis of MI.
{"title":"A study of arginase-1 activity and lipid profile in patients with myocardial infarction","authors":"Ali Anbari, Abdulsamie ee, S. Saad","doi":"10.5455/jabet.2022.d135","DOIUrl":"https://doi.org/10.5455/jabet.2022.d135","url":null,"abstract":"Myocardial necrosis caused by ischemia is called a myocardial infarction (MI). which interrupts coronary blood supply. When the oxygen supply to the heart is insufficient to meet metabolic demands, myocardial ischemia occurs. Atherosclerosis, which obstructs the coronary arteries, is the most common underlying cause of myocardial ischemia. The role of arginase-1 (ARG-1) and serum lipids in the pathogenesis of myocardial infarction is becoming clearer. This study aims to see if there is a link between ARG-1 activity and MI in the Iraqi population. Between the first of November 2021 and the first of February 2022, 90 people were separated into two groups: 45 patients with MI and 45 healthy controls. Human ARG-1 was measured in serum blood using the ELISA method. The serum lipid was measured using the spectrophotometry technique. The current investigation discovered a substantial (p=0.01) rise in ARG-1 concentration compared to control groups, as well as a significant difference in blood lipid content between patients and control groups (p<0.05). Finally, ARG-1 may have a role to play role in the pathogenesis of MI.","PeriodicalId":36275,"journal":{"name":"Journal of Advanced Biotechnology and Experimental Therapeutics","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70796569","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Harika Maganti, Prabu Thandapani, Ragulprasath Kailasam, Adithi Pisapati, Akshaya Bala, A. Mendonça, S. Sundaresan
Insulin Resistance is a pathophysiological function of Type II Diabetes Mellitus which can be comprehended by quantifying the parameters critical in the insulin signaling pathway. Serum has a profound role in evaluating cellular growth and metabolism in vitro. The growth factors present in serum such as IGF, EGF, FGF affects the regulatory components of insulin signaling pathway that leads to insulin resistance. This study explores the effect of growth factors present in Fetal Bovine Serum (FBS) in insulin signaling and endothelium regulation in endothelial cells (Ea. hyp926). The dose-dependent and time-dependent treatment of FBS on the cells displayed changes that were detected by MTT and 2-NBDG assays for assessing cell viability and glucose uptake. Spectrophotometric analysis of nitric oxide (NO) and lactate dehydrogenase (LDH) determined vascular homeostasis and no cytotoxic effects of serum, respectively, in endothelial cells. These findings indicate that FBS at higher levels could possibly lead to loss of NO activity which in turn could impair endothelium-mediated dilation. The inhibition of enzymatic activity of eNOS may activate the release of LDH in endothelial cells. In conclusion, our findings indicate a specific concentration of serum enhances insulin signaling and endothelium cell regulation by modulating glucose uptake and NO production.
{"title":"Effect of growth factors present in serum on insulin resistance and endothelial dysfunction in endothelial cells","authors":"Harika Maganti, Prabu Thandapani, Ragulprasath Kailasam, Adithi Pisapati, Akshaya Bala, A. Mendonça, S. Sundaresan","doi":"10.5455/jabet.2022.d141","DOIUrl":"https://doi.org/10.5455/jabet.2022.d141","url":null,"abstract":"Insulin Resistance is a pathophysiological function of Type II Diabetes Mellitus which can be comprehended by quantifying the parameters critical in the insulin signaling pathway. Serum has a profound role in evaluating cellular growth and metabolism in vitro. The growth factors present in serum such as IGF, EGF, FGF affects the regulatory components of insulin signaling pathway that leads to insulin resistance. This study explores the effect of growth factors present in Fetal Bovine Serum (FBS) in insulin signaling and endothelium regulation in endothelial cells (Ea. hyp926). The dose-dependent and time-dependent treatment of FBS on the cells displayed changes that were detected by MTT and 2-NBDG assays for assessing cell viability and glucose uptake. Spectrophotometric analysis of nitric oxide (NO) and lactate dehydrogenase (LDH) determined vascular homeostasis and no cytotoxic effects of serum, respectively, in endothelial cells. These findings indicate that FBS at higher levels could possibly lead to loss of NO activity which in turn could impair endothelium-mediated dilation. The inhibition of enzymatic activity of eNOS may activate the release of LDH in endothelial cells. In conclusion, our findings indicate a specific concentration of serum enhances insulin signaling and endothelium cell regulation by modulating glucose uptake and NO production.","PeriodicalId":36275,"journal":{"name":"Journal of Advanced Biotechnology and Experimental Therapeutics","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70796807","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Zoo-chemical profiling, in vivo toxicity, and in vitro anti-inflammatory properties of Luffariella herdmani marine sponge extract","authors":"Sashini U Kuruppuarachchi, V. Gunathilake","doi":"10.5455/jabet.2022.d114","DOIUrl":"https://doi.org/10.5455/jabet.2022.d114","url":null,"abstract":"","PeriodicalId":36275,"journal":{"name":"Journal of Advanced Biotechnology and Experimental Therapeutics","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70796240","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
J. Ferdose, M. Alam, Anika Tasnim, M. Chowdhury, M. Obaidullah, M. Matin, M. Sayem
{"title":"Evaluation of selective mitis salivarius agar for the isolation of Streptococcus mutans and its resistance pattern in Bangladesh","authors":"J. Ferdose, M. Alam, Anika Tasnim, M. Chowdhury, M. Obaidullah, M. Matin, M. Sayem","doi":"10.5455/jabet.2022.d115","DOIUrl":"https://doi.org/10.5455/jabet.2022.d115","url":null,"abstract":"","PeriodicalId":36275,"journal":{"name":"Journal of Advanced Biotechnology and Experimental Therapeutics","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70796253","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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