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Aberrant methylation of CDKN2A, RASSF1A and WIF1 in sporadic adenocarcinomatous colorectal cancer: Associations with clinicopathological features 散发性腺癌性结直肠癌中CDKN2A、RASSF1A和WIF1的异常甲基化:与临床病理特征的相关性
Q3 Biochemistry, Genetics and Molecular Biology Pub Date : 2021-01-01 DOI: 10.5455/jabet.2021.d131
L. Vuong, Hung Nguyen, Van-Long Truong, Q. Nguyen
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引用次数: 1
Prevalence and population biology of mastitis-causing Streptococcus uberis using an MLST based approach 使用基于MLST的方法研究引起乳腺炎的ubercoccus的患病率和种群生物学
Q3 Biochemistry, Genetics and Molecular Biology Pub Date : 2021-01-01 DOI: 10.5455/jabet.2021.d132
Maqsud Hossain, Arittra Bhattacharjee, Tahmina Tabassum, Aura Rahman, Mohammad Tausiful Islam
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引用次数: 2
Investigation of growth performance, lipid profile and liver histotexure of mice treated with butyric acid 丁酸对小鼠生长性能、脂质及肝脏组织结构的影响
Q3 Biochemistry, Genetics and Molecular Biology Pub Date : 2021-01-01 DOI: 10.5455/jabet.2021.d140
M. Akter, M. Bhuiyan, K. Sujan, Z. Haque, S. Akter, M. Hossain, M. Islam
{"title":"Investigation of growth performance, lipid profile and liver histotexure of mice treated with butyric acid","authors":"M. Akter, M. Bhuiyan, K. Sujan, Z. Haque, S. Akter, M. Hossain, M. Islam","doi":"10.5455/jabet.2021.d140","DOIUrl":"https://doi.org/10.5455/jabet.2021.d140","url":null,"abstract":"","PeriodicalId":36275,"journal":{"name":"Journal of Advanced Biotechnology and Experimental Therapeutics","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70795873","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 1
Comparison of different chronic respiratory diseases in terms of cigarette smoking: A hospital-based case control study on population of Bangladesh 不同慢性呼吸系统疾病在吸烟方面的比较:一项基于医院的孟加拉国人口病例对照研究
Q3 Biochemistry, Genetics and Molecular Biology Pub Date : 2021-01-01 DOI: 10.5455/JABET.2021.D130
Anindya Das, A. Sumit, Debasish Bhowmick
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引用次数: 0
Antinociceptive potential of methanol leaf extracts of Cissampelos parreira (Linn), Lantana camara (Linn) and Ocimum gratissimum (African basil) 西山、大黄和罗勒甲醇叶提取物的抗伤感受力
Q3 Biochemistry, Genetics and Molecular Biology Pub Date : 2021-01-01 DOI: 10.5455/jabet.2021.d135
Susan Nyawira Kimuni, S. Gitahi, E. Njagi, M. Ngugi
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引用次数: 1
An updated review on lumpy skin disease: a perspective of Southeast Asian countries 疙瘩性皮肤病最新综述:以东南亚国家为例
Q3 Biochemistry, Genetics and Molecular Biology Pub Date : 2021-01-01 DOI: 10.5455/jabet.2021.d133
M. Das, M. Chowdhury, S. Akter, Apurbo Mondal, M. Uddin, Md Rahman, Md Rahman
Recently, Lumpy Skin Disease (LSD) has been portrayed as a terrifying threat to cattle in Southeast Asia. A lump like nodules in the external skin and mucous membrane with fever and swollen lymph nodes are the preliminary noticeable clinical signs of this devastating disease. It is commonly an arthropod-borne contagious illness, correspondingly the non-vector spreading through body discharge and infected fomites. The incubation period ranges from one to four weeks leading to viremia. A pronounced socio-economic collapse is driven by reduced quantity and quality of milk, udder infection, thinness, low quality hides, loss of draught power, abortion, infertility, limitation to meat ingestion, higher morbidity, etc. Animals of any age and gender are susceptible to the disease. The morbidity rate varies according to the immune status of animals and frequency of mechanical vectors. Primarily the disease was endemic in most SubSaharan regions of Africa, consequently extent to Middle East, Europe, and Asia. In the South-Eastern part of Asia, the disease has first been introduced in Bangladesh in July 2019 followed by China, India, Nepal, Bhutan, Vietnam, Hong Kong and Myanmar. Bangladesh recorded the maximum attack rate in Chattogram whereas at Cuttack in India. Particular vulnerable locations of other countries are yet to be confirmed. There is no epidemiological proceeding considering the present LSD situation report from rest of Asia. Strict quarantine, vector control, and prophylactic vaccine might be the best remedy for limiting the risk factors of the disease. Future studies should be directed towards determining the true burden of LSD on livestock and its potential risk factors with the perspective of geographic distributions. J Adv Biotechnol Exp Ther. 2021 Sep; 4(3): 322-333 eISSN: 2616-4760, https://doi.org/10.5455/jabet.2021.d133 Published by www.bsmiab.org
最近,肿块性皮肤病(LSD)被描绘成对东南亚牛的可怕威胁。外皮和粘膜的肿块样结节伴发热和淋巴结肿大是这种毁灭性疾病的初步明显临床症状。它通常是一种节肢动物传播的传染病,相应地,通过身体分泌物和受感染的污染物传播的非媒介疾病。潜伏期为一至四周,导致病毒血症。明显的社会经济崩溃是由牛奶的数量和质量下降、乳房感染、消瘦、低质量的兽皮、失去牵引力、流产、不育、肉类摄入限制、更高的发病率等造成的。任何年龄和性别的动物都容易感染这种疾病。发病率因动物免疫状况和机械媒介的频率不同而不同。该病主要在非洲大多数撒哈拉以南地区流行,随后蔓延到中东、欧洲和亚洲。在亚洲东南部,该疾病于2019年7月首先在孟加拉国被引入,随后是中国、印度、尼泊尔、不丹、越南、香港和缅甸。孟加拉国在Chattogram记录了最高的攻击率,而在印度的Cuttack记录了最高的攻击率。其他国家的特定脆弱地点尚未得到确认。考虑到目前亚洲其他地区的LSD情况报告,没有进行流行病学程序。严格的检疫、媒介控制和预防性疫苗可能是限制疾病风险因素的最佳补救措施。未来的研究应着眼于从地理分布的角度确定LSD对牲畜的真正负担及其潜在危险因素。J Adv biotechnology; 2012.09;[4] (3): 322-333 eISSN: 2616-4760, https://doi.org/10.5455/jabet.2021.d133出版单位www.bsmiab.org
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引用次数: 29
Antiviral effect of honey extract Camelyn against SARS-CoV-2 蜂蜜提取物Camelyn对SARS-CoV-2的抗病毒作用
Q3 Biochemistry, Genetics and Molecular Biology Pub Date : 2021-01-01 DOI: 10.5455/jabet.2021.d129
L. Kalėdienė, M. Baz, A. Liubaviciute, G. Biziuleviciene, I. Grabauskyte, R. Bieliauskiene, P. Jovaisas, N. Jurjonas
This study aimed to evaluate the potential antiviral effects of honey extract “Camelyn” against Severe Acute Respiratory Syndrome coronavirus 2 (SARS-CoV-2). The baby hamster kidney cell line 21 (BHK‐21), bone marrow-derived hematopoietic stem cells (HSCs), and splenic cells were used for Camelyn cytotoxicity assay. After the isolation procedures, cell viability was assessed by trypan blue dye exclusion under a microscope using a hemocytometer. The in vitro cell growth rate was carried out using the cell counting Kit 8 (CCK-8) assay. The cells were seeded in growth media with various Camelyn concentrations (35 μg, 50 μg, 70 μg, 100 μg, 150 μg, and 200 μg). The absorbance at 450 nm was determined by the multiplate reader. The antiviral effect was assessed by plaque reduction assay for the determination of drug susceptibility against SARS-CoV-2. Serial dilution of the selected compounds was pre-incubated with 40 to 100 plaque-forming units (PFUs) of SARS-CoV-2. The pre-incubated mix of Camelyn and SARS-CoV-2 was then added to the confluent Vero E6 cells after incubation cells were fixed and stained and the number of PFUs was counted under an inverted microscope and plotted against the logarithm of antiviral concentrations. Our study showed that Camelyn is not cytotoxic, has a stimulatory effect on cell proliferation, and has an inhibitory effect against SARS-CoV-2 with EC50 (half-maximal effective concentration) from 85.7 μg/mL to 192.4 μg/mL depending on product concentration and viral plaque per cell. © 2021 Global Research Online. All rights reserved.
本研究旨在评估蜂蜜提取物“Camelyn”对严重急性呼吸综合征冠状病毒2 (SARS-CoV-2)的潜在抗病毒作用。Camelyn细胞毒性实验采用仓鼠肾细胞系21 (BHK‐21)、骨髓来源造血干细胞(hsc)和脾细胞。分离后,在显微镜下用血细胞计用台盼蓝染色法测定细胞活力。采用细胞计数试剂盒8 (CCK-8)法测定体外细胞生长速率。将细胞接种于不同Camelyn浓度(35、50、70、100、150、200 μg)的培养基中。在450 nm处用多板仪测定吸光度。采用斑块减少法测定对SARS-CoV-2的药物敏感性,评估抗病毒效果。将选定的化合物连续稀释后,与40至100个SARS-CoV-2斑块形成单位(pfu)进行预孵育。将Camelyn和SARS-CoV-2的预孵育混合物加入到融合的Vero E6细胞中,孵育细胞固定并染色,倒置显微镜下计数PFUs的数量,并根据抗病毒浓度的对数绘制。我们的研究表明,Camelyn没有细胞毒性,对细胞增殖有刺激作用,对SARS-CoV-2有抑制作用,EC50(最大有效浓度的一半)在85.7 μg/mL到192.4 μg/mL之间,这取决于产物浓度和每个细胞的病毒斑块。©2021全球研究在线。版权所有。
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引用次数: 6
Prevalence of multidrug resistance patterns of Escherichia coli from suspected urinary tract infection in Mymensingh city, Bangladesh 孟加拉国迈门辛格市疑似尿路感染的大肠杆菌多重耐药模式的流行情况
Q3 Biochemistry, Genetics and Molecular Biology Pub Date : 2021-01-01 DOI: 10.5455/JABET.2021.D126
F. Nobel, S. Akter, Ruksana Jebin, Titash Sarker, M. Rahman, Saad Zamane, Khairul Islam, Saima Sabrina, Nishat Akther, Ashekul Islam, M. Hasan, M. Islam
1Department of Biochemistry and Molecular Biology, Mawlana Bhashani Science and Technology University, Santosh, Tangail-1902, Bangladesh 2Department of Statistics, Mawlana Bhashani Science and Technology University, Santosh, Tangail-1902, Bangladesh 3Department of Biotechnology and Genetic Engineering, Mawlana Bhashani Science and Technology University, Santosh, Tangail-1902, Bangladesh 4Popular Diagnostic Centre, Mymensingh, Bangladesh
1 .生物化学和分子生物学系,毛拉纳巴沙尼科技大学,桑托什,坦噶伊尔(1902年),孟加拉国2 .统计系,桑托什,坦噶伊尔(1902年),孟加拉国3 .生物技术和基因工程系,毛拉纳巴沙尼科技大学,桑托什,坦噶伊尔(1902年),孟加拉国4 .大众诊断中心,迈门辛格,孟加拉国
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引用次数: 6
Assessment of the anti-leukemic and antioxidant potential of the methanol extract of a wild, edible, and novel mushroom, Astraeus hygrometricus, and unraveling its metabolomic profile. 评估一种野生的、可食用的新型蘑菇——湿法黄芪甲醇提取物的抗白血病和抗氧化潜力,并揭示其代谢组学特征。
Q3 Biochemistry, Genetics and Molecular Biology Pub Date : 2021-01-01 DOI: 10.5455/jabet.2021.d138
Amrita Pal, Ribhu Ray, K. Acharya, Santanu Paul
Mushrooms are enriched with a plethora of bioactive molecules that play a vital role in the prevention of human diseases. The balance between ROS generation and cancer growth is one of the main prerequisites for efficient cancer treatment. In this study, to testify the aforesaid theory, five wild edible mushrooms were initially screened based on their anti-proliferative efficiency, and the best mushroom extract was selected for the assessment of their antioxidant potentialities in vitro in various artificially generated free radicals such as DPPH, ABTS+, and by FRAP experiment. The reason behind the antiproliferative potentiality and antioxidant capability of the most potent extract was also correlated by profiling its metabolites through GC-MS analysis. The study reveals, that the methanolic extract of Astraeus hygrometricus is the most potent anti-leukemic extract (IC50 22.7 ±0.23 μg/mL) followed by Serpula sp. (75.7 ±0.44 μg/mL), Phallus sp. (60.53±0.36 μg/mL), Tricholoma sp. (53.76±0.46 μg/mL), Lentinus sp. (58 ±0.13 μg/mL) against the Jurkat cell line with negligible effect on normal PBMC cells isolated from healthy donors. The assessment of the antioxidant profile Astraeus hygrometricus reveals its moderate antioxidant efficacy against several artificially generated free radicals, such as DPPH (76.9±0.16 μg/mL), ABTS+ (142±0.66 μg/mL) and moderate iron chelating efficacy (32.37±2.31μM). The GC-MS analysis of both methanol and ethyl acetate extracts of Astraeus hygrometricus have found 53 and 52 compounds respectively, with wide diverse ranges of chemically classified biomolecules such as alkane, alcohol, fatty acid, organic acid, polycyclic and heterocyclic compounds, amino acid, vitamin, and hormone, etc. with a wide array of biological activity such as anticancer and antioxidant potentiality. In conclusion, it can be said that these wild edible mushroom Astraeus hygrometricus are a repository of novel biomolecules that can be used in the treatment of Leukemia in the future.
蘑菇富含大量的生物活性分子,在预防人类疾病中起着至关重要的作用。活性氧生成和肿瘤生长之间的平衡是有效治疗癌症的主要先决条件之一。为了验证上述理论,本研究对5种野生食用菌的抗增殖能力进行了初步筛选,并选择了最佳的食用菌提取物,对DPPH、ABTS+等各种人工产生的自由基进行了体外抗氧化能力评价,并通过FRAP实验进行了验证。最有效的提取物的抗增殖潜力和抗氧化能力背后的原因也通过GC-MS分析来分析其代谢物。结果表明,湿法黄芪(Astraeus hygrometricus)甲醇提取物对Jurkat细胞系的抑制作用最强(IC50为22.7±0.23 μg/mL),其次为Serpula sp.(75.7±0.44 μg/mL)、Phallus sp.(60.53±0.36 μg/mL)、Tricholoma sp.(53.76±0.46 μg/mL)、Lentinus sp.(58±0.13 μg/mL),对健康供体正常PBMC细胞的抑制作用可以忽略。结果表明,湿法黄芪对DPPH(76.9±0.16 μg/mL)、ABTS+(142±0.66 μg/mL)和铁螯合作用(32.37±2.31μM)具有中等抗氧化活性。通过对湿法黄芪甲醇提取物和乙酸乙酯提取物的GC-MS分析,分别发现53种和52种化合物,具有广泛的化学分类生物分子,如烷烃、醇、脂肪酸、有机酸、多环和杂环化合物、氨基酸、维生素和激素等,具有广泛的生物活性,如抗癌和抗氧化潜力。综上所述,这些野生食用菌是未来可用于治疗白血病的新型生物分子的储存库。
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引用次数: 1
Evaluation of optimum dietary inclusion level of probiotics for potential benefits on intestinal histomorphometry, microbiota, and pH in Japanese Quails 对日本鹌鹑肠道组织形态、微生物群和pH的潜在益处的最佳膳食益生菌添加水平的评估
Q3 Biochemistry, Genetics and Molecular Biology Pub Date : 2021-01-01 DOI: 10.5455/JABET.2021.D127
M. Afrin, M. Sachi, M. Meher, N. Jahan
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引用次数: 2
期刊
Journal of Advanced Biotechnology and Experimental Therapeutics
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