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Silacrown ethers as ion transport modifiers and preliminary observations of cardiovascular cell line response 硅冠醚作为离子转运调节剂及心血管细胞系反应的初步观察。
IF 3.8 2区 化学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY Pub Date : 2024-12-19 DOI: 10.1016/j.jinorgbio.2024.112814
Barry Arkles , David Segarnick , Leandro C. Clementino , Keith H. Pannell , Andrew P. Thomas
Crown ethers have been shown to have physiological effects ascribed to their ionophoric properties. However, high levels of toxicity precluded interest in their evaluation as therapeutic agents. We prepared new silacrown analogs of crown ethers. These initial studies focused on examples of large ring silacrown ethers having at least fourteen ring atoms with at least one lipophilic or hydrophobic substituent on the ring and/or on the silicon atom. The synthesis of silacrown ethers, ionophoric behavior, toxicity studies, and preliminary pharmacodynamic studies in cardiac myocyte cell lines are presented and compared to their carbon analogs. We report the effects of these compounds in HL-1 cells, an atrial muscle cell line with plasma membrane and sarcoplasmic reticulum Ca2+ channels that give rise to spontaneous Ca2+ transients driven by action potentials. Dicyclohexano-18-crown-6 and the silacrown equivalent dimethylsila-17-cyclohexanocrown-6 were both found to rapidly inhibit the Ca2+ transients after acute treatment, and these effects were reversed when extracellular KCl was increased to cause plasma membrane depolarization. The data suggest that the silacrowns can mimic the effects of crown ethers with similar ring sizes, and this appears to be due to their effects on membrane potential and suppression of action potential firing.
冠醚已被证明具有生理效应归因于其电离性的性质。然而,高水平的毒性阻碍了对其作为治疗剂进行评估的兴趣。我们制备了冠醚的新型硅冠类似物。这些最初的研究集中在具有至少14个环原子的大环硅冠醚的例子上,环和/或硅原子上至少有一个亲脂或疏水取代基。介绍了硅冠醚的合成、离子行为、毒性研究和心肌细胞系的初步药理学研究,并与它们的碳类似物进行了比较。我们报告了这些化合物在HL-1细胞中的作用,HL-1细胞是一种心房肌细胞系,具有质膜和肌浆网Ca2+通道,可引起由动作电位驱动的自发Ca2+瞬态。二环己烯-18-冠-6和硅冠等效物二甲基硅烷-17-环己烯-6在急性治疗后均能迅速抑制Ca2+瞬态,当细胞外KCl增加导致质膜去极化时,这些作用被逆转。数据表明,硅冠可以模仿具有相似环大小的冠醚的作用,这似乎是由于它们对膜电位的影响和对动作电位放电的抑制。
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引用次数: 0
Copper complexes induce haem oxygenase-1 (HMOX1) and cause apoptotic cell death in pancreatic cancer cells 铜配合物诱导血红素加氧酶-1 (HMOX1)并引起胰腺癌细胞凋亡。
IF 3.8 2区 化学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY Pub Date : 2024-12-18 DOI: 10.1016/j.jinorgbio.2024.112815
Zakeeya Jhetam , Carla Martins-Furness , Cathy Slabber , Orde Q. Munro , Marietha Nel , Leonie Harmse
Pancreatic ductal adenocarcinoma (PDAC), the most common pancreatic malignancy, has a dismal 5-year survival rate, making palliative chemotherapy the only treatment option. Targeted therapy has limited efficacy in PDAC, underscoring the need for novel therapeutic approaches. The inducible stress-response protein, haem oxygenase-1 (HMOX1), has been implicated in treatment failure in PDAC.
Copper coordination complexes have shown promise as anticancer agents against various cancers, and are associated with apoptotic cell death. The different ligands to which copper is complexed, determine the specificity and efficacy of each complex.
Three different classes of copper complexes were evaluated for anti-cancer activity against AsPC-1 and MIA PaCa-2 pancreatic cancer cell lines. A copper-phenanthroline-theophylline complex (CuPhTh2), a copper-8-aminoquinoline-naphthyl complex (Cu8AqN), and two copper-aromatic-isoindoline complexes (CuAIsI) were effective inhibitors of cell proliferation with clinically relevant IC50 values below 5 μM. The copper complexes caused reactive oxygen species (ROS) formation, promoted annexin-V binding, disrupted the mitochondrial membrane potential (MMP) and activated caspase-9 and caspase-3/7, confirming apoptotic cell death.
Expression of nuclear HMOX1 was increased in both cell lines, with the CuPhTh2 complex being the most active. Inhibition of HMOX1 activity significantly decreased the IC50 values of these copper complexes suggesting that HMOX1 inhibition may alter treatment outcomes in PDAC.
胰腺导管腺癌(PDAC)是最常见的胰腺恶性肿瘤,其5年生存率很低,姑息性化疗是唯一的治疗选择。靶向治疗在PDAC中的疗效有限,强调需要新的治疗方法。诱导应激反应蛋白血红素加氧酶-1 (HMOX1)与PDAC治疗失败有关。铜配位配合物已显示出抗癌药物的前景,并与凋亡细胞死亡有关。铜与不同的配体络合,决定了每种络合物的特异性和有效性。研究了三种不同类型的铜配合物对胰腺癌细胞系AsPC-1和MIA PaCa-2的抗癌活性。一个铜-菲罗啉-茶碱配合物(CuPhTh2)、一个铜-8-氨基喹啉-萘配合物(Cu8AqN)和两个铜-芳香-异吲哚啉配合物(CuAIsI)是有效的细胞增殖抑制剂,其临床相关IC50值低于5 μM。铜配合物引起活性氧(ROS)的形成,促进膜联蛋白v的结合,破坏线粒体膜电位(MMP),激活caspase-9和caspase-3/7,证实凋亡细胞死亡。细胞核HMOX1在两种细胞系中的表达均有所增加,其中CuPhTh2复合物的表达最为活跃。抑制HMOX1活性可显著降低这些铜复合物的IC50值,表明抑制HMOX1可能会改变PDAC的治疗结果。
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引用次数: 0
A critical analysis of the potential of iron heterobimetallic complexes in anticancer research 铁杂双金属配合物在抗癌研究中的潜力的批判性分析。
IF 3.8 2区 化学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY Pub Date : 2024-12-15 DOI: 10.1016/j.jinorgbio.2024.112813
Boglárka Tűz , Isabel Correia , Paulo N. Martinho
Due to their diverse chemical properties and high ability to interact with biological molecules and cellular processes, transition metal-based compounds have emerged as promising candidates for cancer therapy. Iron complexes are among them, however, there is a gap in the comprehensive analysis of heterometallic iron complexes in the anticancer field. This review aims to fill this gap by summarizing recent progress in the study of Fe(II) and Fe(III) heterobimetallic complexes for anticancer applications and to gather important insights and future perspectives, with special emphasis on their theranostic capabilities. Works published between 2014 and 2024 were considered in this critical survey, that covers a range of complex types, including ferrocene in bimetallic complexes with Pt, Pd, Au, Ag, Ru, Rh, Ir, Cu, Re, Sn and Co; organometallic Fe-complexes with Ru and Ag; photoactive metal complexes with Pt and Co; and magnetic resonance imaging contrast agents with Gd and Mn. Studies conducted to determine the modes of action are highlighted and suggest the involvement of the metal species in reactive oxygen species generation within cells, the impact on apoptosis and cell cycle arrest, and many others. By pursuing interdisciplinary research, innovative theranostic platforms with enhanced efficacy, specificity, and clinical relevance can be developed for cancer management.
由于其不同的化学性质和与生物分子和细胞过程相互作用的高能力,过渡金属基化合物已成为癌症治疗的有希望的候选者。铁配合物就是其中之一,但在抗癌领域对异金属铁配合物的综合分析还存在空白。本文旨在通过总结铁(II)和铁(III)杂双金属配合物抗癌应用的最新研究进展来填补这一空白,并收集重要的见解和未来的展望,特别强调它们的治疗能力。在这项重要的调查中,2014年至2024年间发表的作品被考虑在内,涵盖了一系列配合物类型,包括二茂铁与Pt、Pd、Au、Ag、Ru、Rh、Ir、Cu、Re、Sn和Co的双金属配合物;有机金属铁与Ru、Ag配合物;光活性金属配合物与Pt和Co;以及Gd和Mn的磁共振成像造影剂。为确定作用模式而进行的研究被强调,并表明金属物种参与细胞内活性氧的产生,对细胞凋亡和细胞周期阻滞的影响,以及其他许多方面。通过跨学科研究,可以开发出具有更高疗效、特异性和临床相关性的创新治疗平台。
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引用次数: 0
Solution equilibrium and redox properties of metal complexes with 2-formylpyridine guanylhydrazone derivatives: Effect of morpholine and piperazine substitutions 2-formylpyridine guanylhydrazone 衍生物金属配合物的溶液平衡和氧化还原特性:吗啉和哌嗪取代的影响。
IF 3.8 2区 化学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY Pub Date : 2024-12-10 DOI: 10.1016/j.jinorgbio.2024.112812
Gerda T. Gátszegi , Tatsiana V. Petrasheuskaya , Nóra V. May , Bálint Hajdu , Gabriella Spengler , Felix Bacher , Sergiu Shova , Vladimir B. Arion , Éva A. Enyedy
Schiff bases derived from aminoguanidine are extensively investigated for their structural versatility. The tridentate 2-formylpyridine guanylhydrazones act as analogues of 2-formyl or 2-acetylpyridine thiosemicarbazones, where the thioamide unit is replaced by the guanidyl group. Six derivatives of 2-formylpyridine guanylhydrazone were synthesized and their proton dissociation and complex formation processes with Cu(II), Fe(II) and Fe(III) ions were studied using pH-potentiometry, UV–visible, NMR and electron paramagnetic resonance spectroscopic methods. The ligands have substituents such as amine, morpholine, N-methyl-piperazine at different positions of the pyridine ring. The influence of the different structural elements on the solution chemical properties and cytotoxicity has been disclosed. The solid state structure of four ligands was determined by X-ray crystallography. The ligands bind to Cu(II) in a tridentate fashion via an (N,N,N) donor set, forming mono-ligand complexes. However, for ligands with heterocyclic morpholine and piperazine nitrogen atoms in coordination position a tetradentate binding was observed. Despite the additional coordinating donor atom, the stability of these Cu(II) complexes showed little or no increase. The Cu(II), Fe(II) and Fe(III) complexes of the studied 2-formylpyridine guanylhydrazones exhibited significantly lower stability compared to their corresponding 2-formyl or 2-acetylpyridine thiosemicarbazone analogues. The ligands underwent slow partial hydrolysis (and oxidation) in the presence of Cu(II) ions, leading to the formation of new ligands through the reorganization of structural components around the metal ion. Additionally, the studied Cu(II) complexes demonstrated a great propensity for reduction by glutathione. All these features contributed to the finding that these 2-formylpyridine guanylhydrazones and their Cu(II) complexes did not display measurable cytotoxic activity.
氨基胍衍生的希夫碱因其结构的多功能性而被广泛研究。三齿2-甲酰基吡啶鸟酰腙作为2-甲酰基或2-乙酰基吡啶硫代氨基脲的类似物,其中硫酰胺单元被胍基取代。合成了6个2-甲酰基吡啶鸟酰腙衍生物,并利用ph电位法、紫外可见光谱、核磁共振和电子顺磁共振等方法研究了它们与Cu(II)、Fe(II)和Fe(III)离子的质子解离和络合物形成过程。配体在吡啶环的不同位置有胺、啉、n -甲基哌嗪等取代基。揭示了不同结构元素对溶液化学性质和细胞毒性的影响。用x射线晶体学测定了四种配体的固态结构。配体通过(N,N,N)给体集与Cu(II)以三叉戟方式结合,形成单配体配合物。然而,对于杂环啉和哌嗪氮原子在配位位置的配体,观察到四齿结合。尽管增加了配位给体原子,但这些Cu(II)配合物的稳定性几乎没有提高。所研究的2-甲酰基吡啶鸟酰腙的Cu(II)、Fe(II)和Fe(III)配合物的稳定性明显低于相应的2-甲酰基或2-乙酰吡啶硫代氨基脲类似物。配体在Cu(II)离子存在下发生缓慢的部分水解(和氧化),通过金属离子周围结构组分的重组形成新的配体。此外,所研究的Cu(II)配合物显示出谷胱甘肽还原的巨大倾向。所有这些特征有助于发现这些2-甲酰基吡啶鸟酰腙及其Cu(II)配合物没有显示可测量的细胞毒性活性。
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引用次数: 0
Quantification of lysosomal labile Zn2+ and monitoring of Zn2+ efflux using a small-molecule–protein hybrid fluorescent probe 利用小分子-蛋白杂交荧光探针定量溶酶体可溶性 Zn2+ 并监测 Zn2+ 外流。
IF 3.8 2区 化学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY Pub Date : 2024-12-10 DOI: 10.1016/j.jinorgbio.2024.112811
Yuyin Du , Toshiyuki Kowada , EunHye Sung , Rong Liu , Andrei Soloviev , Toshitaka Matsui , Shin Mizukami
Lysosomal labile Zn2+ levels have been unclear. By targeting a small-molecule fluorescent Zn2+ probe, ZnDA-3H, to lysosomes via VAMP7-Halo, the lysosomal labile Zn2+ concentration was determined to be 1.9 nM in HeLa cells. Furthermore, ZnDA-3H enabled direct visualization of the Zn2+ efflux from the lysosomes to cytosol upon TRPMLs activation.
溶酶体不稳定的Zn2+水平尚不清楚。通过VAMP7-Halo将ZnDA-3H小分子荧光探针靶向溶酶体,测定HeLa细胞中溶酶体不稳定Zn2+浓度为1.9 nM。此外,ZnDA-3H可以直接可视化trpml激活时溶酶体向胞质中流出的Zn2+。
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引用次数: 0
Reinvestigation of the mechanism of dioxygen activation at a MnII(cyclam) center MnII(cyclam)中心双氧活化机制的再研究。
IF 3.8 2区 化学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY Pub Date : 2024-12-09 DOI: 10.1016/j.jinorgbio.2024.112809
Tarali Devi , Stefan Mebs , Dibya Jyoti Barman , Amanda Opis-Basilio , Michael Haumann , Kallol Ray
This study deals with the unprecedented reactivity of a [(cyclam)MnII(OTf)2] (3-cis; OTf = CF3SO3) with O2, which, depending on the presence or absence of a hydrogen atom donor like 1-hydroxy-2,2,6,6-tetramethyl-piperidine (TEMPO-H), selectively generates di-μ-oxo Mn(III)Mn(IV) (1) or MnIV2 (2) complexes, respectively. Both dimers have been characterized by different techniques including single-crystal X-ray diffraction, X-ray absorption spectroscopy, and electron paramagnetic resonance. Oxygenation reactions carried out with labeled 18O2 and Resonance Raman spectroscopy unambiguously show that the oxygen atoms present in the MnIVMnIII dimer originate from O2. Experimental evidences are provided for a novel method of dioxygen activation involving three Mn ions or two Mn ions and TEMPO-H to generate the bis(μ-oxo)dimanganese(IV) or bis(μ-oxo) dimanganese(III, IV) cores, respectively.
本文研究了a [(cyclam)MnII(OTf)2] (3-cis;OTf = CF3SO3-)与O2反应,根据是否存在像1-羟基-2,2,6,6-四甲基哌啶(TEMPO-H)这样的氢原子供体,分别选择性地生成di-μ-oxo Mn(III)Mn(IV)(1)或MnIV2(2)配合物。这两种二聚体都用不同的技术进行了表征,包括单晶x射线衍射、x射线吸收光谱和电子顺磁共振。用标记的18O2和共振拉曼光谱进行的氧化反应明确地表明,MnIVMnIII二聚体中的氧原子来自O2。实验证明,采用3个Mn离子或2个Mn离子和TEMPO-H分别生成双(μ-oxo)二锰(IV)或双(μ-oxo)二锰(III, IV)核的双氧活化新方法是可行的。
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引用次数: 0
Structural insights into temperature-dependent dynamics of METPsc1, a miniaturized electron-transfer protein 微型电子转移蛋白METPsc1的温度依赖动力学的结构见解。
IF 3.8 2区 化学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY Pub Date : 2024-12-09 DOI: 10.1016/j.jinorgbio.2024.112810
Luigi F. Di Costanzo , Gianmattia Sgueglia , Carla Orlando , Maurizio Polentarutti , Linda Leone , Salvatore La Gatta , Maria De Fenza , Luca De Gioia , Angela Lombardi , Federica Arrigoni , Marco Chino
The design of protein-metal complexes is rapidly advancing, with applications spanning catalysis, sensing, and bioremediation. We report a comprehensive investigation of METPsc1, a Miniaturized Electron Transfer Protein, in complex with cadmium. This study elucidates the impact of metal coordination on protein folding and structural dynamics across temperatures from 100 K to 300 K. Our findings reveal that METPsc1, composed of two similar halves stabilized by intramolecular hydrogen bonds, exhibits a unique “clothespin-like” recoil mechanism. This allows it to adapt to metal ions of varying radii, mirroring the flexibility observed in natural rubredoxins. High-resolution crystallography and molecular dynamics simulations unveil concerted backbone motions and subtle temperature-dependent shifts in side-chain conformations, particularly for residues involved in crystal packing. Notably, CdS bond lengths increase with temperature, correlating with anisotropic motions of the sulfur atoms involved in second-shell hydrogen bonding. This suggests a dynamic role of protein matrix upon redox cycling. These insights into METPsc1 highlight its potential for catalysis and contribute to the designing of artificial metalloproteins with functional plasticity.
蛋白质-金属配合物的设计正在迅速发展,其应用跨越催化、传感和生物修复。我们报道了一项全面的研究,METPsc1,一个小型化的电子转移蛋白,在配合镉。本研究阐明了金属配位对蛋白质折叠和结构动力学的影响,温度范围从100 K到300 K。我们的研究结果表明,METPsc1由分子内氢键稳定的两个相似的半部分组成,表现出独特的“衣夹样”反冲机制。这使得它能够适应不同半径的金属离子,反映了在天然红霉素中观察到的灵活性。高分辨率晶体学和分子动力学模拟揭示了协调一致的骨干运动和微妙的温度依赖侧链构象的变化,特别是涉及晶体包装的残基。值得注意的是,CdS键长随着温度的升高而增加,这与参与第二壳层氢键的硫原子的各向异性运动有关。这表明蛋白质基质在氧化还原循环中的动态作用。这些对METPsc1的认识突出了其催化作用的潜力,并有助于设计具有功能可塑性的人造金属蛋白。
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引用次数: 0
Induction of ferroptosis of iridium(III) complexes localizing at the mitochondria and lysosome by photodynamic therapy 通过光动力疗法诱导定位于线粒体和溶酶体的铱(III)复合物发生铁变态反应。
IF 3.8 2区 化学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY Pub Date : 2024-12-09 DOI: 10.1016/j.jinorgbio.2024.112808
Yajie Niu , Shuanghui Tang , Jiongbang Li , Chunxia Huang , Yan Yang , Lin Zhou , Yunjun Liu , Xiandong Zeng
In this study, [Ir(ppy)2(DMHBT)](PF6) (ppy = deprotonated 1-phenylpyridine, DMHBT = 10,12-dimethylpteridino[6,7-f][1,10]phenanthroline-11,13-(10,12H)-dione, 8a), [Ir(bzq)2(DMHBT)](PF6) (bzq = deprotonated benzo[h]quinoline, 8b) and [Ir(piq)2(DMHBT)](PF6) (piq = deprotonated 1-phenylisoquinoline, 8c) were synthesized and characterized by HRMS, 13C NMR and 1H NMR. In vitro cytotoxicity experiments showed that 8a, 8b, 8c show moderate cytotoxicity against B16 cells, while the cytotoxicity of the complexes 8a, 8b and 8c toward B16 cells was greatly improved upon light irradiation, which can be used as photosensitizers to exert anticancer efficacy in photodynamic therapy (PDT). After being taken up by cells, 8a, 8b, 8c were localized in the mitochondria, resulting in a large amount of Ca2+ in-flux, a burst release of ROS, a sustained opening of mitochondrial permeability transition pore, and a decrease of the mitochondrial membrane potential, which led to mitochondrial dysfunction and further activation of caspase 3 and Bcl-2 family proteins to induce apoptosis. Overloaded ROS reacted with polyunsaturated fatty acids on the cell membrane, and initiated lipid peroxidation, inhibited the xc-system-glutathione (GSH)-glutathione peroxidase 4 (GPX4) antioxidant defense system, and upregulated the expression of the damage-associated molecules, HMGB1, CRT, and HSP70. The presence of Fer-1 was effective on increasing the cell survival, which demonstrates that the complexes possess the potential to induce ferroptosis and immunogenic cell death. In addition, 8a, 8b and 8c induced autophagy by inhibiting the AKT/PI3K/mTOR signaling pathway, downregulating p62 and promoting Beclin-1 expression upon light irradiation.
本文合成了[Ir(ppy)2(DMHBT)](PF6) (ppy =去质子化1-苯基吡啶,DMHBT = 10,12-二甲基苯基吡啶[6,7-f][1,10]菲罗啉- 1,13-(10,12h)-二酮,8a), [Ir(bzq)2(DMHBT)](PF6) (bzq =去质子化苯并[h]喹啉,8b)和[Ir(piq)2(DMHBT)](PF6) (piq =去质子化1-苯基异喹啉,8c),并通过HRMS, 13C NMR和1H NMR进行了表征。体外细胞毒实验表明,8a、8b、8c对B16细胞具有中等的细胞毒作用,而8a、8b、8c对B16细胞的细胞毒作用在光照作用下明显增强,可作为光敏剂在光动力治疗(PDT)中发挥抗癌作用。8a、8b、8c被细胞摄取后定位于线粒体,导致Ca2+大量内流,ROS爆发释放,线粒体通透性过渡孔持续打开,线粒体膜电位降低,导致线粒体功能障碍,进一步激活caspase 3和Bcl-2家族蛋白,诱导细胞凋亡。超载的ROS与细胞膜上的多不饱和脂肪酸发生反应,引发脂质过氧化,抑制xc-系统谷胱甘肽(GSH)-谷胱甘肽过氧化物酶4 (GPX4)抗氧化防御系统,上调损伤相关分子HMGB1、CRT和HSP70的表达。铁-1的存在可有效提高细胞存活率,这表明该复合物具有诱导铁下垂和免疫原性细胞死亡的潜力。此外,8a、8b和8c在光照射下通过抑制AKT/PI3K/mTOR信号通路、下调p62和促进Beclin-1表达诱导自噬。
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引用次数: 0
Antioxidant effect, DNA-binding, and transport of the flavonoid acacetin influenced by the presence of redox-active Cu(II) ion: Spectroscopic and in silico study 氧化还原活性铜(II)离子存在对黄酮类化合物 Acacetin 的抗氧化作用、DNA 结合和迁移的影响:光谱和硅学研究。
IF 3.8 2区 化学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY Pub Date : 2024-12-09 DOI: 10.1016/j.jinorgbio.2024.112802
Marek Štekláč , Michal Malček , Peter Gajdoš , Simona Vevericová , Milan Čertík , Marián Valko , Vlasta Brezová , Miriama Malček Šimunková
Acacetin (AC) is a natural polyphenol from the group of flavonoids. It is well established that the behavior of flavonoids depends on the presence of redox-active substances; therefore, we aim to investigate their biological activity following the interaction with Cu(II) ion. Our study demonstrates that AC can effectively bind Cu(II) ions, as confirmed by UV–Vis and EPR spectroscopy as well as DFT calculations. AC appears as a potent scavenger against the model ABTS radical cation by itself, but this ability is significantly limited upon Cu(II) coordination. The possible mild synergistic effect of AC in the presence of vitamin C and glutathione was also shown by the ABTS•+ test. In contrast, an inhibitory effect was observed in the presence of Cu(II) ions. The equimolar addition of AC to the model Fenton-like system containing Cu(II) did not have a noticeable effect on the concentration of hydroxyl radicals produced, but in its excess the formation of OH decreased, as proved by EPR spin trapping. Absorption titrations and gel electrophoresis revealed effective binding to calf thymus (CT)-DNA with a stronger interaction for the Cu(II)-AC complex. The detailed mode of binding to biomolecules was described using molecular docking and molecular dynamics. Obtained results indicate that the double helix of DNA unwinds after interaction with the Cu(II)-AC complex. Fluorescence spectroscopy, employing human serum albumin (HSA), suggested a potential transport capacity for both AC and its Cu(II) complex.
Acacetin (AC)是黄酮类化合物中的一种天然多酚。黄酮类化合物的行为取决于氧化还原活性物质的存在;因此,我们的目标是研究它们与Cu(II)离子相互作用后的生物活性。我们的研究表明,AC可以有效地结合Cu(II)离子,这得到了UV-Vis和EPR光谱以及DFT计算的证实。AC本身似乎是一种有效的ABTS自由基阳离子清除剂,但这种能力在Cu(II)配位时明显受到限制。ABTS•+试验也显示了AC在维生素C和谷胱甘肽存在下可能的轻微协同作用。相反,在Cu(II)离子存在下观察到抑制作用。在含Cu(II)的类fenton模型体系中等量加入AC对羟基自由基的产生没有明显影响,但过量时•OH的形成减少,EPR自旋俘获证实了这一点。吸附滴定和凝胶电泳显示,与小牛胸腺(CT)-DNA有效结合,对Cu(II)-AC络合物具有较强的相互作用。利用分子对接和分子动力学描述了与生物分子的详细结合模式。结果表明,DNA的双螺旋在与Cu(II)-AC配合物相互作用后展开。利用人血清白蛋白(HSA)的荧光光谱分析表明,AC及其Cu(II)配合物具有潜在的转运能力。
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引用次数: 0
Unraveling the mechanism: How does β-phosphoglucomutase from the haloacid dehalogenase superfamily catalyze the interconversion of β-d-glucose 1-phosphate and β-d-glucose 6-phosphate? A chemical perspective 揭开机制:来自卤酸脱卤酶超家族的β-磷酸葡萄糖糖糖化酶是如何催化β-d-葡萄糖1-磷酸和β-d-葡萄糖6-磷酸的相互转化的?从化学的角度来看。
IF 3.8 2区 化学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY Pub Date : 2024-12-04 DOI: 10.1016/j.jinorgbio.2024.112794
Hao Zhang , Mingming Zhang , Kangning Zhu , Yulan Feng , Ling Yang , Wanjian Ding
The catalytic mechanisms of enzymes can be phylogenetically mapped corresponding to their catalytic structures. This mapping effectively elucidates the diversity of enzyme catalytic mechanisms and the emergence of new enzymatic activities within enzyme superfamilies. The haloacid dehalogenase (HAD) superfamily serves as an exemplary model system for comprehending the co-evolution of catalytic structures and mechanisms. This study delves into the mechanism underlying the functional divergence of β-phosphoglucomutase (β-PGM) from the phosphatase branch of the HAD superfamily, employing a chemical perspective. Through the construction and calculation of three models of varying scales using the Density Functional Theory method with B3LYP function, we aim to investigate the chemical mechanism driving this functional divergence of β-PGM from the HAD family. The computational results indicate that residues His20 and Lys76 in the second shell stabilize substrates and enhance the acid-base catalytic ability of Asp10. Additionally, residues Arg49, Ser116 and Asn118 facilitate substrate binding by engaging in close hydrogen bonding interactions with the substrates. Through cooperative action, these residues enable β-PGM to function as an efficient phosphoglucomutase. Through computational modeling and a chemical perspective, we unravel the mechanisms enabling β-PGM to convert β-d-glucose 1-phosphate to β-d-glucose 6-phosphate. Finally, based on the analysis of the evolutionary tree, we discussed and summarized the evolutionary relationships among different forms of metal cores of hydrolases.
酶的催化机制可以根据其催化结构进行系统发育映射。这种图谱有效地阐明了酶催化机制的多样性和酶超家族中新的酶活性的出现。卤酸脱卤酶(HAD)超家族可以作为理解催化结构和机制共同进化的示范模型系统。本研究从化学角度探讨了β-磷酸葡萄糖糖糖化酶(β-PGM)与HAD超家族磷酸酶分支功能分化的机制。利用B3LYP函数的密度泛函理论方法构建和计算三个不同尺度的模型,探讨HAD家族β-PGM功能分化的化学机制。计算结果表明,第二壳的His20和Lys76残基稳定了底物,增强了Asp10的酸碱催化能力。此外,残基Arg49、Ser116和Asn118通过与底物紧密的氢键相互作用促进底物结合。通过协同作用,这些残基使β-PGM作为一种高效的磷酸葡萄糖糖糖化酶发挥作用。通过计算模型和化学角度,我们揭示了β-PGM将β-d-葡萄糖1-磷酸转化为β-d-葡萄糖6-磷酸的机制。最后,在进化树分析的基础上,对不同形态的水解酶金属核的进化关系进行了讨论和总结。
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Journal of Inorganic Biochemistry
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