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Q1 Biochemistry, Genetics and Molecular Biology Pub Date : 2023-12-06 eCollection Date: 2023-01-01 DOI: 10.1080/23328940.2023.2270304
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引用次数: 0
Consumption of hot protein-containing drink accelerates gastric emptying rate and is associated with higher hunger levels in older adults 饮用含蛋白质的热饮可加快胃排空速度,并与老年人饥饿感增加有关
Q1 Biochemistry, Genetics and Molecular Biology Pub Date : 2023-11-19 DOI: 10.1080/23328940.2023.2278410
Kyoko Fujihira, Masaki Takahashi, Chunyi Wang, Saeka Fuke, Naoyuki Hayashi
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引用次数: 0
Living in Western Australia induces some physiological and perceptual adaptations of seasonal acclimatization in the surgical burns team 生活在西澳大利亚诱发一些生理和知觉适应的季节适应外科烧伤团队
Q1 Biochemistry, Genetics and Molecular Biology Pub Date : 2023-11-07 DOI: 10.1080/23328940.2023.2281210
Zehra Palejwala, Karen E. Wallman, Grant J. Landers, Prashan Anbalagan, Fiona M. Wood, Shane K. Maloney
ABSTRACTSeasonal acclimatization is known to result in adaptations that can improve heat tolerance. Staff who operate on burn injuries are exposed to thermally stressful conditions and seasonal acclimatization may improve their thermoeffector responses during surgery. Therefore, the aim of this study was to assess the physiological and perceptual responses of staff who operate on burn injuries during summer and winter, to determine whether they become acclimatized to the heated operating theater. Eight staff members had physiological and perceptual responses compared during burn surgeries conducted in thermoneutral (CON: 24.1 ± 1.2°C, 45 ± 7% relative humidity [RH]) and heated (HOT: 31.3 ± 1.6°C, 44 ± 7% RH) operating theaters, in summer and winter. Physiological parameters that were assessed included core temperature, heart rate, total sweat loss, sweat rate, and urinary specific gravity. Perceptual responses included ratings of thermal sensation and comfort. In summer CON compared to winter CON, baseline (85 ± 15 bpm VS 94 ± 18 bpm), mean (84 ± 16 bpm VS 93 ± 18 bpm), and peak HR (94 ± 17 bpm VS 105 ± 19 bpm) were lower (p < 0.05), whereas core temperature was not different between seasons in either condition (p > 0.05). In HOT, ratings of comfort were higher in summer (15 ± 3) than winter (13 ± 3; p > 0.05), but ratings of thermal sensation and sweat rate were similar between seasons (p > 0.05). The surgical team in burns in Western Australia can obtain some of the physiological and perceptual adaptations that result from seasonal acclimatization, but not all. That is most likely due to a lower than required amount of outdoor heat exposure in summer, to induce all physiological and perceptual adaptations.KEYWORDS: Seasonal acclimatizationburn surgeryadaptationsheat toleranceheat stresscore temperatureheart rateDisclaimerAs a service to authors and researchers we are providing this version of an accepted manuscript (AM). Copyediting, typesetting, and review of the resulting proofs will be undertaken on this manuscript before final publication of the Version of Record (VoR). During production and pre-press, errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal relate to these versions also. AcknowledgementsThe authors thank the burns team at Fiona Stanley Hospital for their participation and the Fiona Wood Foundation for their support with the research.ABBREVIATIONSHR=Heart rateIPAQ=International physical activity questionnaireMET=Metabolic equivalentOT=Operating theatrePPE=Personal protective equipmentRH=Relative humidityTBSA=Total body surface areaTC=Thermal comfortTcore=Core temperatureTS=Thermal sensationUSG=Urinary specific gravityWVP=Water vapour pressureDisclosure statementThe authors report there are no competing interests to declare.Data availabilityThe data that supports the findings of this study are available from the corresponding author [ZP] upon reasonable request.Fig 1. Seaso
在特定的时间点和特定的条件下,季节之间的效应大小中等到较大(g = 0.52 - 0.73)。所示的个人和平均数据。这项工作得到了菲奥娜·伍德基金会的支持
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引用次数: 0
Extracting thermodynamic properties from van ’t Hoff plots with emphasis on temperature-sensing ion channels 从范特霍夫图中提取热力学性质,重点是温度传感离子通道
Q1 Biochemistry, Genetics and Molecular Biology Pub Date : 2023-11-02 DOI: 10.1080/23328940.2023.2265962
Jakob T. Bullerjahn, Sonya M. Hanson
ABSTRACTTransient receptor potential (TRP) ion channels are among the most well-studied classes of temperature-sensing molecules. Yet, the molecular mechanism and thermodynamic basis for the temperature sensitivity of TRP channels remains to this day poorly understood. One hypothesis is that the temperature-sensing mechanism can simply be described by a difference in heat capacity between the closed and open channel states. While such a two-state model may be simplistic it nonetheless has descriptive value, in the sense that it can be used to compare overall temperature sensitivity between different channels and mutants. Here, we introduce a mathematical framework based on the two-state model to reliably extract temperature-dependent thermodynamic potentials and heat capacities from measurements of equilibrium constants at different temperatures. Our framework is implemented in an open-source data analysis package that provides a straightforward way to fit both linear and nonlinear van ’t Hoff plots, thus avoiding some of the previous, potentially erroneous, assumptions when extracting thermodynamic variables from TRP channel electrophysiology data.KEYWORDS: TRP channelstemperature sensorsvan ’t Hoff plotsthermodynamicsspline fitting AcknowledgmentsWe thank Dr. Andrés Jara-Oseguera for fruitful discussions and critical comments on the manuscript. The Flatiron Institute is a division of the Simons Foundation.Disclosure statementNo potential conflict of interest was reported by the author(s).Additional informationFundingThis work was supported by the Max-Planck-Gesellschaft (JTB) and the Simons Foundation (SMH).
瞬态受体电位(TRP)离子通道是被研究得最多的一类温度传感分子。然而,TRP通道温度敏感性的分子机制和热力学基础至今仍知之甚少。一种假设是,温度传感机制可以简单地描述为封闭和开放通道状态之间的热容量差异。虽然这种双态模型可能过于简单,但它仍然具有描述价值,因为它可以用于比较不同通道和突变体之间的总体温度敏感性。在这里,我们引入了一个基于双态模型的数学框架,以可靠地从不同温度下的平衡常数测量中提取与温度相关的热力学势和热容。我们的框架是在一个开源的数据分析软件包中实现的,该软件包提供了一种直接的方法来拟合线性和非线性范特霍夫图,从而避免了从TRP通道电生理数据中提取热力学变量时的一些先前可能错误的假设。关键词:TRP通道,温度传感器,van ' t Hoff图,热力学样条拟合感谢我们感谢andr Jara-Oseguera博士对本文进行的富有成效的讨论和重要的评论。熨斗研究所是西蒙斯基金会的一个部门。披露声明作者未报告潜在的利益冲突。本研究得到了马克斯-普朗克协会(JTB)和西蒙斯基金会(SMH)的支持。
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引用次数: 0
Validation of upper thermal thresholds for outdoor sports using thermal physiology modelling. 利用热生理学模型验证户外运动的上热阈值
Q1 Biochemistry, Genetics and Molecular Biology Pub Date : 2023-05-14 eCollection Date: 2024-01-01 DOI: 10.1080/23328940.2023.2210477
Takahiro Oyama, Minoru Fujii, Kenichi Nakajima, Jun'ya Takakura, Yasuaki Hijioka

Thermal safety guidelines with upper thresholds aim to protect athletes' health, yet evidence-based sport-specific thresholds remain unestablished. Experimenting with athletes in severely hot conditions raises ethical concerns, so we used a thermo-physiological model to validate the thresholds of guidelines for outdoor sports. First, the reproducibility of the joint system thermoregulation model (JOS-3) of core temperature has been validated for 18 sports experiments (n = 213) and 11 general exercise experiments (n = 121) using the Bland - Altman analysis. Then, core temperatures were predicted using the JOS-3 in conditions corresponding to the upper thresholds, and if the 90th-99.7th percentile core temperature value (corresponding to 0.3%-10% of the participants) exceeded 40°C, the thresholds were judged as potentially hazardous. Finally, we proposed revisions for sports with potentially hazardous thresholds. As a result, the JOS-3 could simulate core temperature increases in most experiments (27/29) for six sports and general exercises with an accuracy of 0.5°C. The current upper thresholds for marathons, triathlons, and football are potentially hazardous. Suggested revisions, based on specified percentiles, include: Football: revise from wet bulb globe temperature (WBGT) 32°C to 29-31°C or not revise. Marathon: revise from WBGT 28°C to 24-27°C. Triathlon: revise from WBGT 32.2°C to 23-26°C. If conducting sports events under the revised upper thresholds proves difficult, taking measures for a possible high incidence of heat illness becomes crucial, such as placing additional medical resources, assisting heat acclimatization and cooling strategies for participants, and rule changes such as shorter match times and increased breaks.

热安全指南规定了阈值上限,旨在保护运动员的健康,但基于证据的特定运动阈值仍未确定。在酷热条件下对运动员进行实验会引发道德问题,因此我们使用热生理学模型来验证户外运动指南的阈值。首先,使用布兰德-阿尔特曼分析法对 18 项体育实验(n = 213)和 11 项一般运动实验(n = 121)的核心温度关节系统体温调节模型(JOS-3)的可重复性进行了验证。然后,使用 JOS-3 预测了与上阈值相对应的条件下的核心温度,如果第 90-99.7 百分位数核心温度值(相当于 0.3%-10%的参与者)超过 40°C,则阈值被判定为潜在危险。最后,我们对具有潜在危险阈值的运动项目提出了修订建议。因此,JOS-3 可以在大多数实验(27/29)中模拟六种运动和一般运动的核心温度升高,精确度为 0.5°C。目前马拉松、铁人三项和足球的上限阈值具有潜在危险。根据特定百分位数提出的修订建议包括足球:从湿球温度 (WBGT) 32°C 修订为 29-31°C,或不作修订。马拉松:将湿球温度从 28°C 修订为 24-27°C。铁人三项:从湿球温度 32.2°C 修订为 23-26°C。如果在修订后的阈值上限下举办体育赛事证明有困难,那么采取措施应对可能出现的高温热病高发情况就变得至关重要,例如安排额外的医疗资源、协助参赛者适应高温和采取降温策略,以及改变规则,例如缩短比赛时间和增加休息时间。
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引用次数: 0
Seasonal influence on cognitive and psycho-physiological responses to a single 11-h day of work in outdoor mine industry workers. 季节性影响户外采矿业工人对11小时工作的认知和心理生理反应
Q1 Biochemistry, Genetics and Molecular Biology Pub Date : 2023-05-12 eCollection Date: 2023-01-01 DOI: 10.1080/23328940.2023.2208516
Sarah M Taggart, Olivier Girard, Grant J Landers, Ullrich K H Ecker, Karen E Wallman

This study investigated the seasonal effects that working outdoors had on various parameters in mining industry workers over the course of a work-shift. Workers (n = 27) were assessed in summer (33.3 ± 4.2°C, 38 ± 18% RH; n = 13, age = 46 ± 14 y, BMI = 29.1 ± 5.7 kg/m2) and winter (23.6 ± 5.1°C, 39 ± 20% RH; n = 14, age = 44 ± 12 y, BMI = 31.2 ± 4.1 kg/m2). Core temperature and heart-rate were measured continuously (analyzed at five time points), while perceptual measures, cognitive and manual dexterity performance were assessed at various times over an 11-h shift at the start of a 14-day swing. Hydration was assessed (urine specific gravity) pre- and post-shift. Working memory was impaired in summer compared to winter (-10%; p = 0.039), however did not change throughout the shift. Processing efficiency was significantly reduced at 12 pm (-12%; p = 0.005) and 5 pm (-21%; p < 0.001) compared to 9 am, irrespective of season (p > 0.05). Manual dexterity (dominant-hand) improved over the shift (+13%, p = 0.002), but was not different between seasons. Perceived fatigue had no main effect of season or shift. Core temperature, heart-rate, thermal sensation and rating of perceived exertion increased throughout the shift, with only core temperature and thermal sensation showing a seasonal effect (summer: +0.33°C, +18%, respectively; p < 0.002). Notably, 23% of workers in summer and 64% in winter started work significantly dehydrated, with 54% and 64% in summer and winter, respectively, finishing work with significant to serious dehydration. Impairment in working memory in summer combined with high levels of dehydration over the work-shift reinforces the need for workplace education on the importance of hydration and risk of occupation heat stress. Abbreviations: Core temperature: Tc; Fly-in fly-out: FIFO; Ratings of perceived exertion: RPE; Relative humidity: RH; Urinary specific gravity: USG; Wet bulb globe temperature: WBGT.

本研究调查了户外工作对采矿业工人在轮班过程中各种参数的季节性影响。在夏季(33.3 ± 4.2°C,38 ± 18% 相对湿度;n = 13,年龄 = 46 ± 14 岁,体重指数 = 29.1 ± 5.7 kg/m2)和冬季(23.6 ± 5.1°C,39 ± 20% 相对湿度;n = 14,年龄 = 44 ± 12 岁,体重指数 = 31.2 ± 4.1 kg/m2)对工人(n = 27)进行了评估。对核心温度和心率进行了连续测量(在五个时间点进行分析),同时在 14 天轮班开始时的 11 小时轮班期间的不同时间对知觉测量、认知和手部灵活性表现进行了评估。轮班前和轮班后都对水合情况进行了评估(尿液比重)。与冬季相比,夏季的工作记忆能力受损(-10%;p = 0.039),但在整个轮班过程中没有变化。处理效率在中午 12 点(-12%;p = 0.005)和下午 5 点(-21%;p p > 0.05)明显下降。手动灵活性(优势手)在轮班期间有所改善(+13%,p = 0.002),但在不同季节没有差异。感知疲劳与季节或轮班无关。核心体温、心率、热感和体力感知等级在整个轮班期间都有所上升,只有核心体温和热感有季节性影响(夏季:+0.33°C,+18°C,+18°C):分别为 +0.33°C、+18%;P 缩写:核心温度:Tc;飞入飞出:FIFO;RPE;Relative humidity:相对湿度:相对湿度尿比重USG;湿球温度:湿球温度:WBGT。
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引用次数: 2
About the Cover 关于封面
Q1 Biochemistry, Genetics and Molecular Biology Pub Date : 2023-04-03 DOI: 10.1016/b978-0-12-336156-1.50007-0
E. Ostby
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引用次数: 0
Elevations in serum brain-derived neurotrophic factor following occupational heat stress are not influenced by age or common chronic disease. 职业热应激后血清脑源性神经营养因子的升高不受年龄或常见慢性病的影响
Q1 Biochemistry, Genetics and Molecular Biology Pub Date : 2023-02-23 eCollection Date: 2023-01-01 DOI: 10.1080/23328940.2023.2176107
Nicholas Goulet, James J McCormick, Kelli E King, Sean R Notley, Gary S Goldfield, Naoto Fujii, Tatsuro Amano, Glen P Kenny

With global warming, workers are increasingly exposed to strenuous occupations in hot environments. Given age- and disease-associated declines in thermoregulatory function, older workers are at an elevated risk of developing heat-related injuries. Brain-derived neurotrophic factor (BDNF) is thought to confer neuroprotection during acute exercise, however, the influence of environmental heat on BDNF responses during prolonged work remains unclear. Therefore, we evaluated serum BDNF concentrations before and after 180 min of moderate-intensity treadmill walking (200 W/m2) and after 60 min of post-exercise recovery in temperate (wet-bulb globe temperature (WBGT) 16°C) and hot (WBGT 32°C) environments in 13 healthy young men (mean [SD; 22 [3] years), 12 healthy older men (59 [4] years), 10 men with hypertension (HTN) (60 [4] years), and 9 men with type 2 diabetes (T2D) (60 [5] years). In the temperate condition, all but one participant (1 HTN) completed the 180 min of exercise. While exercise tolerance in the heat was lower in older men with HTN (117 min [45]) and T2D (123 min [42]) compared to healthy older men (159 min [31]) (both p ≤ 0.049), similar end-exercise rectal temperatures (38.9°C [0.4]) were observed across groups, paralleled by similar elevations in serum BDNF across groups at end-exercise (+1106 pg/mL [203]) and end-recovery (+938 pg/mL [146]; all p ≤ 0.01) in the heat. No changes in serum BDNF were observed in the temperate condition. Our findings indicate similar BDNF responses in individuals with HTN or T2D compared to their healthy counterparts, despite exhibiting reduced tolerance to heat.

随着全球变暖,工人们越来越多地暴露在高温环境中从事繁重的工作。由于年龄和疾病导致体温调节功能下降,老年工人发生热相关损伤的风险也随之升高。脑源性神经营养因子(BDNF)被认为能在急性运动时提供神经保护,但环境高温对长期工作时 BDNF 反应的影响仍不清楚。因此,我们在温带(湿球温度(WBGT)16°C)和高温(WBGT 32°C)环境下,对 13 名健康年轻男性(平均 [SD;22[3]岁)、12 名健康老年男性(59[4]岁)、10 名高血压男性(60[4]岁)和 9 名 2 型糖尿病男性(60[5]岁)。在温热条件下,除一人(1 名高血压患者)外,所有参与者都完成了 180 分钟的运动。与健康老年男性(159 分钟 [31])相比,患有高血压的老年男性(117 分钟 [45])和患有 T2D 的老年男性(123 分钟 [42])在高温下的运动耐受力较低(均 p ≤ 0.049),但运动结束时的直肠温度(38.与此同时,各组在运动结束时(+1106 pg/mL [203])和恢复结束时(+938 pg/mL [146];所有 p ≤ 0.01)的血清 BDNF 升高也相似。在温带条件下,血清中的 BDNF 没有变化。我们的研究结果表明,尽管高血压和糖尿病患者对热的耐受性降低,但他们的 BDNF 反应与健康人相似。
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引用次数: 0
Hot water immersion acutely reduces peripheral glucose uptake in young healthy males: An exploratory crossover randomized controlled trial. 热水浸泡可显著降低年轻健康男性的外周血糖摄取:一项探索性交叉随机对照试验
Q1 Biochemistry, Genetics and Molecular Biology Pub Date : 2023-01-08 eCollection Date: 2023-01-01 DOI: 10.1080/23328940.2022.2161242
Matthew J Maley, Andrew P Hunt, Ian B Stewart, Steven Weier, Justin Holland, Christof A Leicht, Geoffrey M Minett

Whether glucose concentration increases during heat exposure because of reduced peripheral tissue uptake or enhanced appearance is currently unknown. This study aimed to report glucose concentrations in both capillary and venous blood in response to a glucose challenge during passive heating (PH) to assess whether heat exposure affects glucose uptake in healthy males. Twelve healthy male participants completed two experimental sessions, where they were asked to undertake an oral glucose tolerance test (OGTT) whilst immersed in thermoneutral (CON, 35.9 (0.6) °C) and hot water (HWI, 40.3 (0.5) °C) for 120 min. Venous and capillary blood [glucose], rectal temperature, and heart rate were recorded. [Glucose] area under the curve for HWI venous (907 (104) AU) differed from CON venous (719 (88) AU, all P < 0.001). No other differences were noted (P > 0.05). Compared with CON, HWI resulted in greater rectal temperature (37.1 (0.3) °C versus 38.6 (0.4) °C, respectively) and heart rate (69 (12) bpm versus 108 (11) bpm, respectively) on cessation (P < 0.001). An OGTT results in similar capillary [glucose] during hot and thermoneutral water immersion, whereas venous [glucose] was greater during HWI when compared with CON. This indicates that peripheral tissue glucose uptake is acutely reduced in response to HWI. Abbreviations: AUC: Area under the curve; CON: Thermoneutral immersion trial; HWI: Hot water immersion trial; OGTT: Oral glucose tolerance test; PH: Passive heating; T-msk: Mean skin temperature; Trec: Rectal temperature.

目前还不清楚暴露于热量时葡萄糖浓度升高是由于外周组织摄取减少还是外观增强。本研究旨在报告毛细血管和静脉血中葡萄糖浓度对被动加热(PH)过程中葡萄糖挑战的反应,以评估热暴露是否会影响健康男性的葡萄糖摄取。12 名健康男性参与者完成了两个实验环节,他们被要求在中温水(CON,35.9 (0.6) °C)和热水(HWI,40.3 (0.5) °C)中浸泡 120 分钟后进行口服葡萄糖耐量试验(OGTT)。记录静脉和毛细血管血液[葡萄糖]、直肠温度和心率。HWI 静脉血[葡萄糖]曲线下面积(907 (104) AU)与 CON 静脉血(719 (88) AU,均为 P 0.05)不同。与 CON 相比,停止 HWI 时直肠温度(分别为 37.1 (0.3) °C 和 38.6 (0.4) °C)和心率(分别为 69 (12) bpm 和 108 (11) bpm)更高(P 缩写:缩写:AUC:缩写:AUC:曲线下面积;CON:热中性浸泡试验;HWI:热水浸泡试验;OGTT:口服葡萄糖耐量试验;PH:被动加热;T-msk:平均皮肤温度;Trec:直肠温度。
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引用次数: 1
The effectiveness of heat preparation and alleviation strategies for cognitive performance: A systematic review. 热准备和缓解策略对认知表现的有效性:一项系统综述
Q1 Biochemistry, Genetics and Molecular Biology Pub Date : 2023-01-04 eCollection Date: 2023-01-01 DOI: 10.1080/23328940.2022.2157645
Kate J Donnan, Emily L Williams, Melissa J Bargh

A range of occupational and performance contexts (e.g. military personnel operations, emergency services, sport) require the critical maintenance of cognitive performance in environmentally challenging environments. Several reviews exist which evaluate the effectiveness of heat preparation strategies to facilitate physical performance. To date, no review has explored the usefulness of heat preparation strategies for cognitive performance. Therefore, this systematic review aimed to evaluate a range of interventions for the maintenance of cognitive performance, during or following active or passive heat exposure. Studies to be included were assessed by two authors reviewing title, abstract, and full-text. Forty articles were identified which met the inclusion criteria. Interventions were categorised into chronic (i.e. acclimation/acclimatisation) and acute strategies (i.e. hydration, cooling, supplementation, psychological). The results indicate that medium-term consecutive heat acclimation may mitigate some cognitive deficits under heat stress, although heat acclimation effectiveness could be influenced by age. Further, pre-cooling appears the most effective cooling method for maintaining cognitive performance under heat stress, although results were somewhat ambiguous. The hydration literature showed that the most effective hydration strategies were those which individualised electrolyte fortified fluid volumes to match for sweat loss. Limited research exploring psychological interventions indicates that motivational self-talk could be facilitative for maintaining cognitive skills following exercise in hot conditions. These findings can be used to help inform strategies for maintaining critical cognitive and decision-making skills in hot environments.

一系列职业和表现环境(如军事人员行动、应急服务、体育运动)都需要在环境挑战环境中保持关键的认知表现。目前已有多篇综述对促进体能表现的热准备策略的有效性进行了评估。迄今为止,还没有一篇综述探讨了热备策略对认知表现的作用。因此,本系统性综述旨在评估一系列在主动或被动高温暴露期间或之后维持认知能力的干预措施。拟纳入的研究由两位作者通过审查标题、摘要和全文进行评估。最终确定了 40 篇符合纳入标准的文章。干预措施分为慢性(即适应/气候化)和急性策略(即补水、降温、补充、心理)。结果表明,虽然热适应的效果可能受年龄影响,但中期连续热适应可减轻热应激下的一些认知缺陷。此外,预冷似乎是在热应激下保持认知能力最有效的降温方法,尽管结果有些模糊。水合文献显示,最有效的水合策略是那些个性化的电解质强化液体量,以适应汗液流失的策略。对心理干预措施进行的有限研究表明,激励性自我对话有助于在高温条件下运动后保持认知技能。这些研究结果可用于帮助制定在炎热环境中保持关键认知和决策技能的策略。
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引用次数: 0
期刊
Temperature
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