Pub Date : 2026-05-01Epub Date: 2025-11-19DOI: 10.1016/j.aaf.2025.10.003
Dahai Gao , Yan Zhang , Hongchang Ding , Xinghong Yan
Several strains with long-type blade have been isolated from Pyropia suborbiculata, showing application potential for commercial Pyropia cultivation. In this study, using strain PS-M4 of P. suborbiculata, the effects of temperatures and light levels on conchocelis growth, conchosporangia formation, and conchospores release, were examined. The optimal conditions for conchocelis growth were 24 °C and 30–40 μmol photons/(m2·s), whereas the conchosporangia formation ratio peaked at 30 °C and 10–20 μmol photons/(m2·s), yielding the highest conchospore release under these conditions. Compared with strain PH-WT10 of P. haitanensis, the conchocelis of PS-M4 displayed higher thermotolerance and superior conchospore yield. These attributes underscore the suitability of PS-M4 for convenient and climate-resilient conchocelis cultivation.
{"title":"Effects of temperature and light level on the growth and development of conchocelis in Pyropia suborbiculata (Bangiales, Rhodophyta)","authors":"Dahai Gao , Yan Zhang , Hongchang Ding , Xinghong Yan","doi":"10.1016/j.aaf.2025.10.003","DOIUrl":"10.1016/j.aaf.2025.10.003","url":null,"abstract":"<div><div>Several strains with long-type blade have been isolated from <em>Pyropia suborbiculata</em>, showing application potential for commercial <em>Pyropia</em> cultivation. In this study, using strain <em>PS-M4</em> of <em>P. suborbiculata</em>, the effects of temperatures and light levels on conchocelis growth, conchosporangia formation, and conchospores release, were examined. The optimal conditions for conchocelis growth were 24 °C and 30–40 μmol photons/(m<sup>2</sup>·s), whereas the conchosporangia formation ratio peaked at 30 °C and 10–20 μmol photons/(m<sup>2</sup>·s), yielding the highest conchospore release under these conditions. Compared with strain <em>PH-WT10</em> of <em>P. haitanensis</em>, the conchocelis of <em>PS-M4</em> displayed higher thermotolerance and superior conchospore yield. These attributes underscore the suitability of <em>PS-M4</em> for convenient and climate-resilient conchocelis cultivation.</div></div>","PeriodicalId":36894,"journal":{"name":"Aquaculture and Fisheries","volume":"11 3","pages":"Pages 612-617"},"PeriodicalIF":0.0,"publicationDate":"2026-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146026080","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2026-05-01Epub Date: 2025-12-23DOI: 10.1016/j.aaf.2025.11.009
S. Janani, M. Hemalatha, D.S. Dipshika, M. Sukumar
Penaeus monodon (giant tiger prawn), one of the most valuable aquaculture species, provides a great deal of nutrition, sensory properties, and importance as a marketable food species. Spoilage occurs quickly and presents serious issues of post-harvest quality, safety, and shelf life. Preservation methods that are well-established, such as freezing, chilling, and adding chemical preservatives, may decrease quality, reduce nutritional value or result in a public health issue if any chemicals remain as residues. In response to the rising consumer demand for minimally-processed, safe, and sustainable seafood products, green and hybrid preservation methods have gained considerable interest. This review provides exhaustive coverage of several novel preservation methods examined for Penaeus monodon, including combinations of non-thermal methods (such as pulsed electric fields, ultrasound, and cold plasma), natural preservatives (such as plant extracts, essential oils, and bioactive coatings), and advanced forms of packaging. Furthermore, much of the focus in this review will be on hybrid methods that encourage synergism for inhibiting microorganisms, preventing lipid oxidation, and preserving sensory attributes of the seafood throughout storage. This article intends to provide information to those in the seafood industry, seafood technologists, and research community in promoting preservation methods to establish sustainability and supply for Penaeus monodon. Despite promising lab scale findings, challenges with customer acceptance, regulatory approvals, and process optimization prevent the various preservation strategies from commercial scaling. Variability in microbial load, size, and lack of harmonized protocols further complicate quality assurance and regulatory approval. Future research should focus on understanding mechanical relationships, optimizing multi-hurdle combinations, pilot scale validation, collaboration with industry stakeholders, meeting customer expectations, and clear regulatory frameworks. Additionally, developing non-destructive, rapid, environmentally friendly freshness assessment techniques that are more accessible than traditional chemical methods represents an important research direction.
{"title":"Hybrid and green preservation techniques for Penaeus monodon: A review of emerging technologies, shelf life enhancement and research gaps","authors":"S. Janani, M. Hemalatha, D.S. Dipshika, M. Sukumar","doi":"10.1016/j.aaf.2025.11.009","DOIUrl":"10.1016/j.aaf.2025.11.009","url":null,"abstract":"<div><div><em>Penaeus monodon</em> (giant tiger prawn), one of the most valuable aquaculture species, provides a great deal of nutrition, sensory properties, and importance as a marketable food species. Spoilage occurs quickly and presents serious issues of post-harvest quality, safety, and shelf life. Preservation methods that are well-established, such as freezing, chilling, and adding chemical preservatives, may decrease quality, reduce nutritional value or result in a public health issue if any chemicals remain as residues. In response to the rising consumer demand for minimally-processed, safe, and sustainable seafood products, green and hybrid preservation methods have gained considerable interest. This review provides exhaustive coverage of several novel preservation methods examined for <em>Penaeus monodon,</em> including combinations of non-thermal methods (such as pulsed electric fields, ultrasound, and cold plasma), natural preservatives (such as plant extracts, essential oils, and bioactive coatings), and advanced forms of packaging. Furthermore, much of the focus in this review will be on hybrid methods that encourage synergism for inhibiting microorganisms, preventing lipid oxidation, and preserving sensory attributes of the seafood throughout storage. This article intends to provide information to those in the seafood industry, seafood technologists, and research community in promoting preservation methods to establish sustainability and supply for <em>Penaeus monodon.</em> Despite promising lab scale findings, challenges with customer acceptance, regulatory approvals, and process optimization prevent the various preservation strategies from commercial scaling. Variability in microbial load, size, and lack of harmonized protocols further complicate quality assurance and regulatory approval. Future research should focus on understanding mechanical relationships, optimizing multi-hurdle combinations, pilot scale validation, collaboration with industry stakeholders, meeting customer expectations, and clear regulatory frameworks. Additionally, developing non-destructive, rapid, environmentally friendly freshness assessment techniques that are more accessible than traditional chemical methods represents an important research direction.</div></div>","PeriodicalId":36894,"journal":{"name":"Aquaculture and Fisheries","volume":"11 3","pages":"Pages 499-509"},"PeriodicalIF":0.0,"publicationDate":"2026-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146025803","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Due to the increasing pollution of aquatic environments by estrogen-like chemicals (xenoestrogens (XEs)), it is crucial to investigate their bioaccumulation, ecological impact, and potential endocrine-disrupting effects on aquatic organisms. Here, we investigated the effects of 17β-estradiol (E2) and the XEs bisphenol A (BPA) and diethylstilbestrol (DES) on the nervous system, with a particular focus on dopaminergic (DAergic) neurons during early development of zebrafish. Our results revealed that a low dose of E2 (10−4 μM) significantly increased tyrosine hydroxylase (TH), the rate-limiting enzyme for dopamine (DA) synthesis, at 48 hpf, whereas a high dose (1 μM) significantly reduced TH expression. A similar pattern was observed for both BPA and DES, with DES exhibiting a more potent effect compared to BPA and E2. Specifically, DES increased TH expression at 10−4 μM and reduced TH expression at concentrations starting from 0.1 μM, whereas BPA increased TH expression at 10−3 μM and reduced TH expression at 1 μM. These effects were further validated through qRT-PCR analysis. The changes in TH expression correlated with alterations in motor activity, including the response to tactile stimulation at 72 hpf and swimming distance at 6 dpf, except that low doses had no effect on swimming distance. Notably, all effects caused by E2, BPA, or DES, at both low and high doses, were mediated through estrogen receptors (ER). While replacing E2 with embryonic medium (EM) did not rescue the effect on locomotor activity, replacing or adding L-dopa (a DA precursor) completely rescued the effect. Moreover, co-incubation with buspirone (a partial agonist for serotonin (5-HT) 1A receptors) resulted in partial rescue, indicating that both DA and 5-HT signaling are involved in modulating locomotor activity, with DAergic neurons playing a central role. In conclusion, we demonstrated that E2 and XEs regulate DA neurons through ER in a biphasic manner, modulating locomotor activity during early zebrafish development. Our findings thus highlight the potential toxicological impact of XEs, as their disruption on DAergic neurons and estrogen signaling pathways can lead to altered motor behavior, developmental abnormalities, and long-term neurological effects.
{"title":"Biphasic effects of 17β-estradiol and xenoestrogens on dopaminergic neurons in developing zebrafish","authors":"Ratu Fatimah , Zulvikar Syambani Ulhaq , Meshkatul Jannat , Sugiyono , Mitsuyo Kishida","doi":"10.1016/j.aaf.2025.08.011","DOIUrl":"10.1016/j.aaf.2025.08.011","url":null,"abstract":"<div><div>Due to the increasing pollution of aquatic environments by estrogen-like chemicals (xenoestrogens (XEs)), it is crucial to investigate their bioaccumulation, ecological impact, and potential endocrine-disrupting effects on aquatic organisms. Here, we investigated the effects of 17β-estradiol (E<sub>2</sub>) and the XEs bisphenol A (BPA) and diethylstilbestrol (DES) on the nervous system, with a particular focus on dopaminergic (DAergic) neurons during early development of zebrafish. Our results revealed that a low dose of E<sub>2</sub> (10<sup>−4</sup> μM) significantly increased tyrosine hydroxylase (TH), the rate-limiting enzyme for dopamine (DA) synthesis, at 48 hpf, whereas a high dose (1 μM) significantly reduced TH expression. A similar pattern was observed for both BPA and DES, with DES exhibiting a more potent effect compared to BPA and E<sub>2</sub>. Specifically, DES increased TH expression at 10<sup>−4</sup> μM and reduced TH expression at concentrations starting from 0.1 μM, whereas BPA increased TH expression at 10<sup>−3</sup> μM and reduced TH expression at 1 μM. These effects were further validated through qRT-PCR analysis. The changes in TH expression correlated with alterations in motor activity, including the response to tactile stimulation at 72 hpf and swimming distance at 6 dpf, except that low doses had no effect on swimming distance. Notably, all effects caused by E<sub>2</sub>, BPA, or DES, at both low and high doses, were mediated through estrogen receptors (ER). While replacing E<sub>2</sub> with embryonic medium (EM) did not rescue the effect on locomotor activity, replacing or adding L-dopa (a DA precursor) completely rescued the effect. Moreover, co-incubation with buspirone (a partial agonist for serotonin (5-HT) 1A receptors) resulted in partial rescue, indicating that both DA and 5-HT signaling are involved in modulating locomotor activity, with DAergic neurons playing a central role. In conclusion, we demonstrated that E<sub>2</sub> and XEs regulate DA neurons through ER in a biphasic manner, modulating locomotor activity during early zebrafish development. Our findings thus highlight the potential toxicological impact of XEs, as their disruption on DAergic neurons and estrogen signaling pathways can lead to altered motor behavior, developmental abnormalities, and long-term neurological effects.</div></div>","PeriodicalId":36894,"journal":{"name":"Aquaculture and Fisheries","volume":"11 3","pages":"Pages 510-518"},"PeriodicalIF":0.0,"publicationDate":"2026-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146026270","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Wnt genes play crucial roles in various biological mechanisms, such as cell signaling, development, and tissue homeostasis. Recent studies have highlighted the critical role of Wnt genes in limb regeneration. However, the identification and characterization of Wnt genes in the Chinese mitten crab (Eriocheir sinensis) remains unexplored. In this study, we conducted a whole-genome identification of Wnts in E. sinensis, and analyzing the sequence characteristics and expression patterns. In summary, 29 Wnt genes were identified in E. sinensis and classed into eight groups based on the sequence similarity. Notably, Wnt7 gene in E. sinensis exists expansion of species-specific. Chromosome location analysis revealed that 14 Wnts were located on chromosomes while the remaining genes were mapped to scaffold segments. Gene structure analysis revealed that Wnt genes contain 10 conserved motifs and the Wnt domain, indicating the conservation of Wnt genes. RNA-seq results further revealed that Wnt5 and Wnt11 may function in limb regeneration. Overall, these findings provide new insights for further functional characterization of Wnts, highlighting the complex mechanism of the Wnts in the regulation of limb regeneration.
{"title":"Identification, sequence characteristics, and expression patterns of Wnt genes in Eriocheir sinensis","authors":"Maolei Wei , Xinxin Chen , Xirui Zheng , Qi Zhu , Dongran Yang , Xugan Wu , Xiaowu Chen","doi":"10.1016/j.aaf.2025.08.006","DOIUrl":"10.1016/j.aaf.2025.08.006","url":null,"abstract":"<div><div><em>Wnt</em> genes play crucial roles in various biological mechanisms, such as cell signaling, development, and tissue homeostasis. Recent studies have highlighted the critical role of <em>Wnt</em> genes in limb regeneration. However, the identification and characterization of <em>Wnt</em> genes in the Chinese mitten crab (<em>Eriocheir sinensis</em>) remains unexplored. In this study, we conducted a whole-genome identification of <em>Wnts</em> in <em>E</em>. <em>sinensis</em>, and analyzing the sequence characteristics and expression patterns. In summary, 29 <em>Wnt</em> genes were identified in <em>E. sinensis</em> and classed into eight groups based on the sequence similarity. Notably, <em>Wnt7</em> gene in <em>E. sinensis</em> exists expansion of species-specific. Chromosome location analysis revealed that 14 <em>Wnts</em> were located on chromosomes while the remaining genes were mapped to scaffold segments. Gene structure analysis revealed that <em>Wnt</em> genes contain 10 conserved motifs and the <em>Wnt</em> domain, indicating the conservation of <em>Wnt</em> genes. RNA-seq results further revealed that <em>Wnt5</em> and <em>Wnt11</em> may function in limb regeneration. Overall, these findings provide new insights for further functional characterization of <em>Wnts</em>, highlighting the complex mechanism of the <em>Wnts</em> in the regulation of limb regeneration.</div></div>","PeriodicalId":36894,"journal":{"name":"Aquaculture and Fisheries","volume":"11 3","pages":"Pages 554-562"},"PeriodicalIF":0.0,"publicationDate":"2026-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146026268","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2026-05-01Epub Date: 2025-12-16DOI: 10.1016/j.aaf.2025.11.005
Min Huang , Ke Cheng , Chuyi Zhang , Yijia Wang , Gang Yang , Xiaoyan Hu , Chunfang Wang
This study investigated the immunomodulatory role of dietary vitamin D3 (VD3) in hybrid yellow catfish under high-density culture (HDC). A 2 × 3 factorial design was applied with two stocking densities and three VD3 levels (0, 1120, 14900 IU/kg). HDC significantly increased mortality, oxidative stress, lipid peroxidation, and liver Fe2+ accumulation, exhibiting ferroptosis-related features. VD3 supplementation alleviated these effects by reducing lipid peroxidation, modulating antioxidant enzymes, and activating the Keap1–Nrf2 pathway. It also upregulated gpx4 and regulated iron/lipid metabolism, thereby improving iron homeostasis and mitigating ferroptosis. Notably, VD3 downregulated slc40a1 under HDC, suggesting that its regulatory mechanism is context dependent. Within the tested conditions, 1120 IU/kg VD3 showed more pronounced protective effects against oxidative damage and ferroptosis. By contrast, under HDC, 14 900 IU/kg tended to act as an additional stressor. These findings provide new insights into the context-dependent role of VD3 in alleviating high-density stress and sustaining antioxidant balance in fish.
{"title":"Vitamin D3 supplementation mitigates oxidative stress and ferroptosis, enhancing liver, spleen, and head-kidney health and growth performance in high-density cultured hybrid yellow catfish (Pelteobagrus fulvidraco♀ × Pelteobagrus vachelli♂)","authors":"Min Huang , Ke Cheng , Chuyi Zhang , Yijia Wang , Gang Yang , Xiaoyan Hu , Chunfang Wang","doi":"10.1016/j.aaf.2025.11.005","DOIUrl":"10.1016/j.aaf.2025.11.005","url":null,"abstract":"<div><div>This study investigated the immunomodulatory role of dietary vitamin D<sub>3</sub> (VD<sub>3</sub>) in hybrid yellow catfish under high-density culture (HDC). A 2 × 3 factorial design was applied with two stocking densities and three VD<sub>3</sub> levels (0, 1120, 14900 IU/kg). HDC significantly increased mortality, oxidative stress, lipid peroxidation, and liver Fe<sup>2+</sup> accumulation, exhibiting ferroptosis-related features. VD<sub>3</sub> supplementation alleviated these effects by reducing lipid peroxidation, modulating antioxidant enzymes, and activating the Keap1–Nrf2 pathway. It also upregulated <em>gpx4</em> and regulated iron/lipid metabolism, thereby improving iron homeostasis and mitigating ferroptosis. Notably, VD<sub>3</sub> downregulated <em>slc40a1</em> under HDC, suggesting that its regulatory mechanism is context dependent. Within the tested conditions, 1120 IU/kg VD<sub>3</sub> showed more pronounced protective effects against oxidative damage and ferroptosis. By contrast, under HDC, 14 900 IU/kg tended to act as an additional stressor. These findings provide new insights into the context-dependent role of VD<sub>3</sub> in alleviating high-density stress and sustaining antioxidant balance in fish.</div></div>","PeriodicalId":36894,"journal":{"name":"Aquaculture and Fisheries","volume":"11 3","pages":"Pages 563-575"},"PeriodicalIF":0.0,"publicationDate":"2026-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146025805","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2026-05-01Epub Date: 2025-12-24DOI: 10.1016/j.aaf.2025.11.003
Peng Huang , Jinliang Du , Jie Wei , Jiancao Gao , Liping Cao , Jun Gao , Jiayi Li , Haojun Zhu , Yao Zheng , Gangchun Xu , Shunlong Meng
Ovarian development in the Chinese mitten crab (Eriocheir sinensis) enters a rapid development period after the reproductive molt. During this process, low-temperature stimulation seems to be a key initiating factor. To verify this, the current study simulated a nine-week long-term low-temperature experiment during the fattening stage in an indoor thermostatic water circulation system, including a high-temperature control group (30 °C), a medium-temperature group (25 °C) and low-temperature group (20 °C). Based on multi-omics sequencing and histological analysis, we found that chronic low-temperature exposure promotes ovarian development by up-regulating the mRNA expression of crucial genes (e75/74, vg/vgr, 17β-estradiol, erα) and promoting the secretion of GIH, MF, and E2 hormones. This can be evidenced by the densely distributed yolk granules, significantly increased oocyte diameter, and GSI index. Interestingly, the significantly increased lipid droplet area and crude lipid level indicate that chronic cold stimulation can promote fat accumulation in the ovary. Significantly, the peroxisome proliferator-activated receptor (PPAR) signaling pathway and phospholipids, including phosphatidylcholine (PC) and phosphatidylethanolamine (PE), play important roles in this process. In addition, continuous cold exposure can significantly increase the levels of monounsaturated fatty acids (MUFAs) and polyunsaturated fatty acids (PUFAs) of hepatopancreas and ovarian tissue, and inhibit the synthesis of saturated fatty acids (SFAs) to promote the deposition of nutrients and flavor substances. In conclusion, our current data reveal a series of physiological responses to continuous cold exposure during fattening, providing valuable data support for the fattening strategy and quality improvement of Chinese mitten crabs.
{"title":"Effects of different temperatures on ovarian development, lipid metabolism and nutritional quality of Eriocheir sinensis during fattening","authors":"Peng Huang , Jinliang Du , Jie Wei , Jiancao Gao , Liping Cao , Jun Gao , Jiayi Li , Haojun Zhu , Yao Zheng , Gangchun Xu , Shunlong Meng","doi":"10.1016/j.aaf.2025.11.003","DOIUrl":"10.1016/j.aaf.2025.11.003","url":null,"abstract":"<div><div>Ovarian development in the Chinese mitten crab (<em>Eriocheir sinensis</em>) enters a rapid development period after the reproductive molt. During this process, low-temperature stimulation seems to be a key initiating factor. To verify this, the current study simulated a nine-week long-term low-temperature experiment during the fattening stage in an indoor thermostatic water circulation system, including a high-temperature control group (30 °C), a medium-temperature group (25 °C) and low-temperature group (20 °C). Based on multi-omics sequencing and histological analysis, we found that chronic low-temperature exposure promotes ovarian development by up-regulating the mRNA expression of crucial genes (<em>e75</em>/<em>74</em>, <em>vg</em>/<em>vgr</em>, <em>17β-estradiol</em>, <em>erα</em>) and promoting the secretion of GIH, MF, and E2 hormones. This can be evidenced by the densely distributed yolk granules, significantly increased oocyte diameter, and GSI index. Interestingly, the significantly increased lipid droplet area and crude lipid level indicate that chronic cold stimulation can promote fat accumulation in the ovary. Significantly, the peroxisome proliferator-activated receptor (PPAR) signaling pathway and phospholipids, including phosphatidylcholine (PC) and phosphatidylethanolamine (PE), play important roles in this process. In addition, continuous cold exposure can significantly increase the levels of monounsaturated fatty acids (MUFAs) and polyunsaturated fatty acids (PUFAs) of hepatopancreas and ovarian tissue, and inhibit the synthesis of saturated fatty acids (SFAs) to promote the deposition of nutrients and flavor substances. In conclusion, our current data reveal a series of physiological responses to continuous cold exposure during fattening, providing valuable data support for the fattening strategy and quality improvement of Chinese mitten crabs.</div></div>","PeriodicalId":36894,"journal":{"name":"Aquaculture and Fisheries","volume":"11 3","pages":"Pages 540-553"},"PeriodicalIF":0.0,"publicationDate":"2026-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146025802","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2026-05-01Epub Date: 2025-09-29DOI: 10.1016/j.aaf.2025.07.001
Yaqi Jin , Ganfeng Yi , Na Jin , Liqiu Shi , Yucai Xue , Xuxiong Huang
Commercial live microalgae products have gained popularity in aquaculture hatcheries and farms due to their convenience, versatility, and high nutrition. This study investigated the effects of different storage temperatures and durations on the cell composition and growth performance of microalga Auxenochlorella pyrenoidosa. The results showed that the shelf life of A. pyrenoidosa was 7 days at room temperature or 14 days at 4 °C. Cellular carbohydrate content initially decreased and then increased, while cellular protein content continuously increased. Total lipid contents decreased with prolonged storage. At room temperature, the total lipid contents of 14 d (13.23%), 21d (13.03%) and 28 d (11.69%) treatments were significantly lower than those of the 7 d treatment (15.17%) and the control (15.65%). At 4 °C, the total lipid contents of the 28 d treatment (11.66%) was considerably lower than that of the control. No significant difference in cell density was observed among the control, the treatment of 7 d (1.62 × 107 cells/mL) at room temperature, and the treatment of 14 d (1.70 × 107 cells/mL) at 4 °C after re-inoculated for 14 days of culture. A significant correlation was identified between the cellular total lipid content of stored microalgae and the vitality of re-inoculated cells, suggesting that a significant decrease in cellular lipid content could serve as an index for rapid identification of the growth performance of microalgae post-shelf life. These findings provide valuable technical insights for the application of microalgae in aquaculture.
商业微藻产品因其方便、多用途和高营养而在水产养殖场和养殖场中受到欢迎。本试验研究了不同贮藏温度和贮存时间对乳酸菌微藻细胞组成和生长性能的影响。结果表明，乳酸菌在室温下的保质期为7 d，在4℃下的保质期为14 d。细胞碳水化合物含量先降低后升高，细胞蛋白质含量不断升高。总脂质含量随着储存时间的延长而降低。室温下，处理14 d（13.23%）、21d（13.03%）和28 d（11.69%）的总脂肪含量显著低于处理7 d（15.17%）和对照（15.65%）。在4°C时，处理28 d的总脂质含量（11.66%）显著低于对照。对照组、室温下处理7 d （1.62 × 107个细胞/mL）和4℃下处理14 d （1.70 × 107个细胞/mL）培养14 d后细胞密度无显著差异。贮藏微藻的细胞总脂含量与再接种细胞的活力呈显著相关，表明细胞总脂含量的显著降低可以作为快速鉴定微藻货架期后生长性能的指标。这些发现为微藻在水产养殖中的应用提供了有价值的技术见解。
{"title":"Effects of storage temperature and duration on cell composition and growth performance of microalga Auxenochlorella pyrenoidosa","authors":"Yaqi Jin , Ganfeng Yi , Na Jin , Liqiu Shi , Yucai Xue , Xuxiong Huang","doi":"10.1016/j.aaf.2025.07.001","DOIUrl":"10.1016/j.aaf.2025.07.001","url":null,"abstract":"<div><div>Commercial live microalgae products have gained popularity in aquaculture hatcheries and farms due to their convenience, versatility, and high nutrition. This study investigated the effects of different storage temperatures and durations on the cell composition and growth performance of microalga <em>Auxenochlorella pyrenoidosa</em>. The results showed that the shelf life of <em>A. pyrenoidosa</em> was 7 days at room temperature or 14 days at 4 °C. Cellular carbohydrate content initially decreased and then increased, while cellular protein content continuously increased. Total lipid contents decreased with prolonged storage. At room temperature, the total lipid contents of 14 d (13.23%), 21d (13.03%) and 28 d (11.69%) treatments were significantly lower than those of the 7 d treatment (15.17%) and the control (15.65%). At 4 °C, the total lipid contents of the 28 d treatment (11.66%) was considerably lower than that of the control. No significant difference in cell density was observed among the control, the treatment of 7 d (1.62 × 10<sup>7</sup> cells/mL) at room temperature, and the treatment of 14 d (1.70 × 10<sup>7</sup> cells/mL) at 4 °C after re-inoculated for 14 days of culture. A significant correlation was identified between the cellular total lipid content of stored microalgae and the vitality of re-inoculated cells, suggesting that a significant decrease in cellular lipid content could serve as an index for rapid identification of the growth performance of microalgae post-shelf life. These findings provide valuable technical insights for the application of microalgae in aquaculture.</div></div>","PeriodicalId":36894,"journal":{"name":"Aquaculture and Fisheries","volume":"11 3","pages":"Pages 587-594"},"PeriodicalIF":0.0,"publicationDate":"2026-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146026083","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2026-03-01Epub Date: 2025-10-28DOI: 10.1016/j.aaf.2025.09.006
Tung Hoang , Binh Thai Nguyen
Sustainable development of the global shrimp industry relies on not only consistent supplies of domesticated broodstock, but also their commercially viable spawning performance. In this study we collected 13 sets of maturation data from three commercial shrimp hatcheries in Vietnam to review the reproductive performance with and without eyestalk ablation of both domesticated Penaeus vannamei and domesticated P. monodon. Our results showed that in 2024 unablated P. vannamei outperformed their ablated counterparts, producing 8.3 million nauplii/female (from 21 to 27 spawns/female) or nearly four folds higher than that of ablated females over an exploitation of 126–148 days. Spawning rate of unablated P. vannamei females was high, averaging 21.6 % nightly. A positive growth rate of 0.29 g/day was recorded for unablated P. vannamei indicating good shrimp health. Furthermore, temporal comparisons revealed a significant improvement in reproductive performance of unablated P. vannamei females (supplied by the same broodstock supplier) from 2015 to 2024 regarding spawning rate (48 % from 14.6 % to 21.6 % nightly), fecundity (37 % from 250 to 352 × 103 nauplii/spawn) and nauplii yield (113 % from 3.9 to 8.3 × 106 nauplii/female). Induced by eyestalk ablation and artificial insemination domesticated P. monodon females produced 3.3 million nauplii/female on average over 91–102 days of the exploitation period. Average fecundity ranged from 447 to 507 × 103 nauplii/spawn among the broodstock batches. Survival of ablated P. monodon females averaged at 58.8 ± 8.5 %. Both males and ablated females of P. monodon kept growing during the exploitation period at 0.17 ± 0.03 g and 0.14 ± 0.01 g, respectively. Unablated P. monodon females with much lower maturation rate and lower fecundity produced only 0.05 million nauplii/female or 72 folds lower than that of their ablated counterparts. Their very poor reproductive performance suggests that banning eyestalk ablation will make postlarvae production of domesticated P. monodon non-viable economically and impractical technically due to unrealistic number of broodstock to be used and substantial additional resources required. In contrast, spawning domesticated P. vannamei without eyestalk ablation should be encouraged.
{"title":"Spawning performance of domesticated penaeids in commercial hatcheries and possible impacts of eyestalk-ablation ban on postlarvae production","authors":"Tung Hoang , Binh Thai Nguyen","doi":"10.1016/j.aaf.2025.09.006","DOIUrl":"10.1016/j.aaf.2025.09.006","url":null,"abstract":"<div><div>Sustainable development of the global shrimp industry relies on not only consistent supplies of domesticated broodstock, but also their commercially viable spawning performance. In this study we collected 13 sets of maturation data from three commercial shrimp hatcheries in Vietnam to review the reproductive performance with and without eyestalk ablation of both domesticated <em>Penaeus vannamei</em> and domesticated <em>P. monodon</em>. Our results showed that in 2024 unablated <em>P. vannamei</em> outperformed their ablated counterparts, producing 8.3 million nauplii/female (from 21 to 27 spawns/female) or nearly four folds higher than that of ablated females over an exploitation of 126–148 days. Spawning rate of unablated <em>P. vannamei</em> females was high, averaging 21.6 % nightly. A positive growth rate of 0.29 g/day was recorded for unablated <em>P. vannamei</em> indicating good shrimp health. Furthermore, temporal comparisons revealed a significant improvement in reproductive performance of unablated <em>P. vannamei</em> females (supplied by the same broodstock supplier) from 2015 to 2024 regarding spawning rate (48 % from 14.6 % to 21.6 % nightly), fecundity (37 % from 250 to 352 × 10<sup>3</sup> nauplii/spawn) and nauplii yield (113 % from 3.9 to 8.3 × 10<sup>6</sup> nauplii/female). Induced by eyestalk ablation and artificial insemination domesticated <em>P. monodon</em> females produced 3.3 million nauplii/female on average over 91–102 days of the exploitation period. Average fecundity ranged from 447 to 507 × 10<sup>3</sup> nauplii/spawn among the broodstock batches. Survival of ablated <em>P. monodon</em> females averaged at 58.8 ± 8.5 %. Both males and ablated females of <em>P. monodon</em> kept growing during the exploitation period at 0.17 ± 0.03 g and 0.14 ± 0.01 g, respectively. Unablated <em>P. monodon</em> females with much lower maturation rate and lower fecundity produced only 0.05 million nauplii/female or 72 folds lower than that of their ablated counterparts. Their very poor reproductive performance suggests that banning eyestalk ablation will make postlarvae production of domesticated <em>P. monodon</em> non-viable economically and impractical technically due to unrealistic number of broodstock to be used and substantial additional resources required. In contrast, spawning domesticated <em>P. vannamei</em> without eyestalk ablation should be encouraged.</div></div>","PeriodicalId":36894,"journal":{"name":"Aquaculture and Fisheries","volume":"11 2","pages":"Pages 351-360"},"PeriodicalIF":0.0,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145842296","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2026-03-01Epub Date: 2025-08-26DOI: 10.1016/j.aaf.2025.08.005
Shuhang Lv , Changsheng Gao , Yongchao Li , Yunze Zhang , Hongtao Nie
Salinity is one of the important factors affecting the immune function of aquatic animals, and Vibrio anguillarum is one of the most important pathogens in aquaculture industry. In this study, we investigated the physiological, biochemical and molecular responses of Ruditapes philippinarum to salinity and V. anguillarum stress through different salinity combined with V. anguillarum challenge treatments. The results showed that ATP-binding cassette transporter A2 (ABCA2) gene, lysosomal integral membrane protein-2 (LIMP) gene, and mannose-6-phosphate receptors (MPR) gene were mainly involved in immune responses in gill tissues, whereas prosaposin (PSAP) gene was mainly involved in immune responses in hepatopancreatic tissues. It was also found that a short period of salinity (24–48 h) could enhance the immune response of clams. This study provides a reference for the immune regulation mechanism of bivalves under salinity and V. anguillarum stress.
{"title":"Immune responses and survival of Ruditapes philippinarum under salinity stress and Vibrio anguillarum challenge","authors":"Shuhang Lv , Changsheng Gao , Yongchao Li , Yunze Zhang , Hongtao Nie","doi":"10.1016/j.aaf.2025.08.005","DOIUrl":"10.1016/j.aaf.2025.08.005","url":null,"abstract":"<div><div>Salinity is one of the important factors affecting the immune function of aquatic animals, and <em>Vibrio anguillarum</em> is one of the most important pathogens in aquaculture industry. In this study, we investigated the physiological, biochemical and molecular responses of <em>Ruditapes philippinarum</em> to salinity and <em>V. anguillarum</em> stress through different salinity combined with <em>V. anguillarum</em> challenge treatments. The results showed that <em>ATP-binding cassette transporter A2</em> (<em>ABCA2</em>) gene, <em>lysosomal integral membrane protein-2</em> (<em>LIMP</em>) gene, and <em>mannose-6-phosphate receptors</em> (<em>MPR</em>) gene were mainly involved in immune responses in gill tissues, whereas <em>prosaposin</em> (<em>PSAP</em>) gene was mainly involved in immune responses in hepatopancreatic tissues. It was also found that a short period of salinity (24–48 h) could enhance the immune response of clams. This study provides a reference for the immune regulation mechanism of bivalves under salinity and <em>V. anguillarum</em> stress.</div></div>","PeriodicalId":36894,"journal":{"name":"Aquaculture and Fisheries","volume":"11 2","pages":"Pages 289-297"},"PeriodicalIF":0.0,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145842791","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2026-03-01Epub Date: 2025-08-25DOI: 10.1016/j.aaf.2025.08.009
Jaynos R. Cortes , Ian B. Benitez , Bernajocele Jalyn S. Baldoza , Charm Angel R. Pardillo , Kathleen Mae A. Auxtero , Kristina P. Badec , Daryl Anne B. Varela
This review examines the integration of climate-smart aquaculture (CSAq) as a strategy to enhance the resilience and sustainability of global aquaculture and coastal agriculture in the face of climate change. CSAq encompasses innovations such as integrated multi-trophic aquaculture (IMTA), genetic advancements, renewable energy integration, and optimized water management, all aimed at minimizing environmental impacts while maintaining productivity. As climate change introduces threats like ocean acidification, temperature fluctuations, and extreme weather events, CSAq offers adaptive solutions critical for preserving marine ecosystems, reducing greenhouse gas emissions, and sustaining food security. The review emphasizes that the successful adoption of CSAq is contingent upon supportive policies, cross-sectoral collaboration, and socio-economic considerations, including gender inclusivity and community involvement. As aquaculture's role in food security continues to grow, CSAq provides a pathway for mitigating climate impacts while promoting sustainable development. This review underscores the necessity of climate-smart approaches for building resilient food systems that can adapt to a changing climate and sustain livelihoods in vulnerable coastal regions.
{"title":"Climate-smart aquaculture: Innovations and challenges in mitigating climate change impacts on fisheries and coastal agriculture","authors":"Jaynos R. Cortes , Ian B. Benitez , Bernajocele Jalyn S. Baldoza , Charm Angel R. Pardillo , Kathleen Mae A. Auxtero , Kristina P. Badec , Daryl Anne B. Varela","doi":"10.1016/j.aaf.2025.08.009","DOIUrl":"10.1016/j.aaf.2025.08.009","url":null,"abstract":"<div><div>This review examines the integration of climate-smart aquaculture (CSAq) as a strategy to enhance the resilience and sustainability of global aquaculture and coastal agriculture in the face of climate change. CSAq encompasses innovations such as integrated multi-trophic aquaculture (IMTA), genetic advancements, renewable energy integration, and optimized water management, all aimed at minimizing environmental impacts while maintaining productivity. As climate change introduces threats like ocean acidification, temperature fluctuations, and extreme weather events, CSAq offers adaptive solutions critical for preserving marine ecosystems, reducing greenhouse gas emissions, and sustaining food security. The review emphasizes that the successful adoption of CSAq is contingent upon supportive policies, cross-sectoral collaboration, and socio-economic considerations, including gender inclusivity and community involvement. As aquaculture's role in food security continues to grow, CSAq provides a pathway for mitigating climate impacts while promoting sustainable development. This review underscores the necessity of climate-smart approaches for building resilient food systems that can adapt to a changing climate and sustain livelihoods in vulnerable coastal regions.</div></div>","PeriodicalId":36894,"journal":{"name":"Aquaculture and Fisheries","volume":"11 2","pages":"Pages 221-231"},"PeriodicalIF":0.0,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145842298","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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