Pub Date : 2025-09-04DOI: 10.1016/j.aaf.2025.08.012
Lingwei Tang , Deng Pan , Yizhen Wang , Shuxia Yao , Xueru Qian , Chigang Huang , Fangyuan Peng , Jinghui Liu , Wen Fu , Liangyue Peng , Yamei Xiao , Wenbin Liu
Eutrophication significantly impacts on aquatic ecosystems. The accumulation of nutrients such as nitrogen and phosphorus in water bodies leads to a series of ecological problems, including water quality deterioration, reduced dissolved oxygen levels, and decreased transparency. These issues significantly impact the physical and chemical properties of water bodies, the diversity of aquatic organisms, community structure, and ecological functions. Carassius cuvieri (CC) and Triploid Crucian carp No. 2 (TCC) are common freshwater aquaculture fish species with extensive farming foundations and economic value. Their survival and growth are closely tied to environment conditions. This study uses CC and Triploid TCC as research subjects, conducting a 30-day eutrophication aquaculture experiment in a mildly eutrophic water body (TN: 0.868 mg/L; TP: 0 mg/L) as the initial environment. Combining morphological, histological, transcriptomic, inhibitor-treatment, and qRT-PCR techniques, we explored the molecular mechanisms underlying the adaptation of crucian carp to eutrophication environments. The results showed that, as the degree of eutrophication increased, growth indices of both CC and TCC were affected to varying degrees. Peripheral blood cell and histological section observations revealed abnormal blood cell morphology, increased white blood cell counts, nuclear displacement and aggregation in hepatocytes, curved and thinned gill filaments, and broken and damaged gill filaments in both fish species; High-throughput sequencing results showed that differentially expressed genes in liver tissue were primarily enriched in immune-mediated infectious-diseases, lipid-metabolism, and signal -transduction pathways. mRNA expression analysis of crucian carp embryos treated with the TLR5 inhibitor TH1020 suggested that changes in certain genes within the TLRs signaling pathway may be associated with the activation of downstream cascades and the secretion of pro-inflammatory cytokines, collectively facilitating fish adaptation eutrophication. This study provides scientific experimental evidence for optimising large-scale fish farming models, monitoring of aquaculture environmental quality, and maintaining the balance of aquatic ecosystems. TCC copes better with eutrophication through stronger antioxidant and immune responses, whereas CC shows more pronounced gill and liver damage.
{"title":"Research on the molecular mechanisms of crucian carp adaptation to eutrophication","authors":"Lingwei Tang , Deng Pan , Yizhen Wang , Shuxia Yao , Xueru Qian , Chigang Huang , Fangyuan Peng , Jinghui Liu , Wen Fu , Liangyue Peng , Yamei Xiao , Wenbin Liu","doi":"10.1016/j.aaf.2025.08.012","DOIUrl":"10.1016/j.aaf.2025.08.012","url":null,"abstract":"<div><div>Eutrophication significantly impacts on aquatic ecosystems. The accumulation of nutrients such as nitrogen and phosphorus in water bodies leads to a series of ecological problems, including water quality deterioration, reduced dissolved oxygen levels, and decreased transparency. These issues significantly impact the physical and chemical properties of water bodies, the diversity of aquatic organisms, community structure, and ecological functions. <em>Carassius cuvieri</em> (CC) and Triploid <em>Crucian carp No. 2</em> (TCC) are common freshwater aquaculture fish species with extensive farming foundations and economic value. Their survival and growth are closely tied to environment conditions. This study uses CC and Triploid TCC as research subjects, conducting a 30-day eutrophication aquaculture experiment in a mildly eutrophic water body (TN: 0.868 mg/L; TP: 0 mg/L) as the initial environment. Combining morphological, histological, transcriptomic, inhibitor-treatment, and qRT-PCR techniques, we explored the molecular mechanisms underlying the adaptation of crucian carp to eutrophication environments. The results showed that, as the degree of eutrophication increased, growth indices of both CC and TCC were affected to varying degrees. Peripheral blood cell and histological section observations revealed abnormal blood cell morphology, increased white blood cell counts, nuclear displacement and aggregation in hepatocytes, curved and thinned gill filaments, and broken and damaged gill filaments in both fish species; High-throughput sequencing results showed that differentially expressed genes in liver tissue were primarily enriched in immune-mediated infectious-diseases, lipid-metabolism, and signal -transduction pathways. mRNA expression analysis of crucian carp embryos treated with the <em>TLR5</em> inhibitor TH1020 suggested that changes in certain genes within the TLRs signaling pathway may be associated with the activation of downstream cascades and the secretion of pro-inflammatory cytokines, collectively facilitating fish adaptation eutrophication. This study provides scientific experimental evidence for optimising large-scale fish farming models, monitoring of aquaculture environmental quality, and maintaining the balance of aquatic ecosystems. TCC copes better with eutrophication through stronger antioxidant and immune responses, whereas CC shows more pronounced gill and liver damage.</div></div>","PeriodicalId":36894,"journal":{"name":"Aquaculture and Fisheries","volume":"11 2","pages":"Pages 242-254"},"PeriodicalIF":0.0,"publicationDate":"2025-09-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145842796","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Due to the increasing pollution of aquatic environments by estrogen-like chemicals (xenoestrogens (XEs)), it is crucial to investigate their bioaccumulation, ecological impact, and potential endocrine-disrupting effects on aquatic organisms. Here, we investigated the effects of 17β-estradiol (E2) and the XEs bisphenol A (BPA) and diethylstilbestrol (DES) on the nervous system, with a particular focus on dopaminergic (DAergic) neurons during early development of zebrafish. Our results revealed that a low dose of E2 (10−4 μM) significantly increased tyrosine hydroxylase (TH), the rate-limiting enzyme for dopamine (DA) synthesis, at 48 hpf, whereas a high dose (1 μM) significantly reduced TH expression. A similar pattern was observed for both BPA and DES, with DES exhibiting a more potent effect compared to BPA and E2. Specifically, DES increased TH expression at 10−4 μM and reduced TH expression at concentrations starting from 0.1 μM, whereas BPA increased TH expression at 10−3 μM and reduced TH expression at 1 μM. These effects were further validated through qRT-PCR analysis. The changes in TH expression correlated with alterations in motor activity, including the response to tactile stimulation at 72 hpf and swimming distance at 6 dpf, except that low doses had no effect on swimming distance. Notably, all effects caused by E2, BPA, or DES, at both low and high doses, were mediated through estrogen receptors (ER). While replacing E2 with embryonic medium (EM) did not rescue the effect on locomotor activity, replacing or adding L-dopa (a DA precursor) completely rescued the effect. Moreover, co-incubation with buspirone (a partial agonist for serotonin (5-HT) 1A receptors) resulted in partial rescue, indicating that both DA and 5-HT signaling are involved in modulating locomotor activity, with DAergic neurons playing a central role. In conclusion, we demonstrated that E2 and XEs regulate DA neurons through ER in a biphasic manner, modulating locomotor activity during early zebrafish development. Our findings thus highlight the potential toxicological impact of XEs, as their disruption on DAergic neurons and estrogen signaling pathways can lead to altered motor behavior, developmental abnormalities, and long-term neurological effects.
{"title":"Biphasic effects of 17β-estradiol and xenoestrogens on dopaminergic neurons in developing zebrafish","authors":"Ratu Fatimah , Zulvikar Syambani Ulhaq , Meshkatul Jannat , Sugiyono , Mitsuyo Kishida","doi":"10.1016/j.aaf.2025.08.011","DOIUrl":"10.1016/j.aaf.2025.08.011","url":null,"abstract":"<div><div>Due to the increasing pollution of aquatic environments by estrogen-like chemicals (xenoestrogens (XEs)), it is crucial to investigate their bioaccumulation, ecological impact, and potential endocrine-disrupting effects on aquatic organisms. Here, we investigated the effects of 17β-estradiol (E<sub>2</sub>) and the XEs bisphenol A (BPA) and diethylstilbestrol (DES) on the nervous system, with a particular focus on dopaminergic (DAergic) neurons during early development of zebrafish. Our results revealed that a low dose of E<sub>2</sub> (10<sup>−4</sup> μM) significantly increased tyrosine hydroxylase (TH), the rate-limiting enzyme for dopamine (DA) synthesis, at 48 hpf, whereas a high dose (1 μM) significantly reduced TH expression. A similar pattern was observed for both BPA and DES, with DES exhibiting a more potent effect compared to BPA and E<sub>2</sub>. Specifically, DES increased TH expression at 10<sup>−4</sup> μM and reduced TH expression at concentrations starting from 0.1 μM, whereas BPA increased TH expression at 10<sup>−3</sup> μM and reduced TH expression at 1 μM. These effects were further validated through qRT-PCR analysis. The changes in TH expression correlated with alterations in motor activity, including the response to tactile stimulation at 72 hpf and swimming distance at 6 dpf, except that low doses had no effect on swimming distance. Notably, all effects caused by E<sub>2</sub>, BPA, or DES, at both low and high doses, were mediated through estrogen receptors (ER). While replacing E<sub>2</sub> with embryonic medium (EM) did not rescue the effect on locomotor activity, replacing or adding L-dopa (a DA precursor) completely rescued the effect. Moreover, co-incubation with buspirone (a partial agonist for serotonin (5-HT) 1A receptors) resulted in partial rescue, indicating that both DA and 5-HT signaling are involved in modulating locomotor activity, with DAergic neurons playing a central role. In conclusion, we demonstrated that E<sub>2</sub> and XEs regulate DA neurons through ER in a biphasic manner, modulating locomotor activity during early zebrafish development. Our findings thus highlight the potential toxicological impact of XEs, as their disruption on DAergic neurons and estrogen signaling pathways can lead to altered motor behavior, developmental abnormalities, and long-term neurological effects.</div></div>","PeriodicalId":36894,"journal":{"name":"Aquaculture and Fisheries","volume":"11 3","pages":"Pages 510-518"},"PeriodicalIF":0.0,"publicationDate":"2025-09-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146026270","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Wnt genes play crucial roles in various biological mechanisms, such as cell signaling, development, and tissue homeostasis. Recent studies have highlighted the critical role of Wnt genes in limb regeneration. However, the identification and characterization of Wnt genes in the Chinese mitten crab (Eriocheir sinensis) remains unexplored. In this study, we conducted a whole-genome identification of Wnts in E. sinensis, and analyzing the sequence characteristics and expression patterns. In summary, 29 Wnt genes were identified in E. sinensis and classed into eight groups based on the sequence similarity. Notably, Wnt7 gene in E. sinensis exists expansion of species-specific. Chromosome location analysis revealed that 14 Wnts were located on chromosomes while the remaining genes were mapped to scaffold segments. Gene structure analysis revealed that Wnt genes contain 10 conserved motifs and the Wnt domain, indicating the conservation of Wnt genes. RNA-seq results further revealed that Wnt5 and Wnt11 may function in limb regeneration. Overall, these findings provide new insights for further functional characterization of Wnts, highlighting the complex mechanism of the Wnts in the regulation of limb regeneration.
{"title":"Identification, sequence characteristics, and expression patterns of Wnt genes in Eriocheir sinensis","authors":"Maolei Wei , Xinxin Chen , Xirui Zheng , Qi Zhu , Dongran Yang , Xugan Wu , Xiaowu Chen","doi":"10.1016/j.aaf.2025.08.006","DOIUrl":"10.1016/j.aaf.2025.08.006","url":null,"abstract":"<div><div><em>Wnt</em> genes play crucial roles in various biological mechanisms, such as cell signaling, development, and tissue homeostasis. Recent studies have highlighted the critical role of <em>Wnt</em> genes in limb regeneration. However, the identification and characterization of <em>Wnt</em> genes in the Chinese mitten crab (<em>Eriocheir sinensis</em>) remains unexplored. In this study, we conducted a whole-genome identification of <em>Wnts</em> in <em>E</em>. <em>sinensis</em>, and analyzing the sequence characteristics and expression patterns. In summary, 29 <em>Wnt</em> genes were identified in <em>E. sinensis</em> and classed into eight groups based on the sequence similarity. Notably, <em>Wnt7</em> gene in <em>E. sinensis</em> exists expansion of species-specific. Chromosome location analysis revealed that 14 <em>Wnts</em> were located on chromosomes while the remaining genes were mapped to scaffold segments. Gene structure analysis revealed that <em>Wnt</em> genes contain 10 conserved motifs and the <em>Wnt</em> domain, indicating the conservation of <em>Wnt</em> genes. RNA-seq results further revealed that <em>Wnt5</em> and <em>Wnt11</em> may function in limb regeneration. Overall, these findings provide new insights for further functional characterization of <em>Wnts</em>, highlighting the complex mechanism of the <em>Wnts</em> in the regulation of limb regeneration.</div></div>","PeriodicalId":36894,"journal":{"name":"Aquaculture and Fisheries","volume":"11 3","pages":"Pages 554-562"},"PeriodicalIF":0.0,"publicationDate":"2025-09-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146026268","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-08-26DOI: 10.1016/j.aaf.2025.08.005
Shuhang Lv , Changsheng Gao , Yongchao Li , Yunze Zhang , Hongtao Nie
Salinity is one of the important factors affecting the immune function of aquatic animals, and Vibrio anguillarum is one of the most important pathogens in aquaculture industry. In this study, we investigated the physiological, biochemical and molecular responses of Ruditapes philippinarum to salinity and V. anguillarum stress through different salinity combined with V. anguillarum challenge treatments. The results showed that ATP-binding cassette transporter A2 (ABCA2) gene, lysosomal integral membrane protein-2 (LIMP) gene, and mannose-6-phosphate receptors (MPR) gene were mainly involved in immune responses in gill tissues, whereas prosaposin (PSAP) gene was mainly involved in immune responses in hepatopancreatic tissues. It was also found that a short period of salinity (24–48 h) could enhance the immune response of clams. This study provides a reference for the immune regulation mechanism of bivalves under salinity and V. anguillarum stress.
{"title":"Immune responses and survival of Ruditapes philippinarum under salinity stress and Vibrio anguillarum challenge","authors":"Shuhang Lv , Changsheng Gao , Yongchao Li , Yunze Zhang , Hongtao Nie","doi":"10.1016/j.aaf.2025.08.005","DOIUrl":"10.1016/j.aaf.2025.08.005","url":null,"abstract":"<div><div>Salinity is one of the important factors affecting the immune function of aquatic animals, and <em>Vibrio anguillarum</em> is one of the most important pathogens in aquaculture industry. In this study, we investigated the physiological, biochemical and molecular responses of <em>Ruditapes philippinarum</em> to salinity and <em>V. anguillarum</em> stress through different salinity combined with <em>V. anguillarum</em> challenge treatments. The results showed that <em>ATP-binding cassette transporter A2</em> (<em>ABCA2</em>) gene, <em>lysosomal integral membrane protein-2</em> (<em>LIMP</em>) gene, and <em>mannose-6-phosphate receptors</em> (<em>MPR</em>) gene were mainly involved in immune responses in gill tissues, whereas <em>prosaposin</em> (<em>PSAP</em>) gene was mainly involved in immune responses in hepatopancreatic tissues. It was also found that a short period of salinity (24–48 h) could enhance the immune response of clams. This study provides a reference for the immune regulation mechanism of bivalves under salinity and <em>V. anguillarum</em> stress.</div></div>","PeriodicalId":36894,"journal":{"name":"Aquaculture and Fisheries","volume":"11 2","pages":"Pages 289-297"},"PeriodicalIF":0.0,"publicationDate":"2025-08-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145842791","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-08-25DOI: 10.1016/j.aaf.2025.08.009
Jaynos R. Cortes , Ian B. Benitez , Bernajocele Jalyn S. Baldoza , Charm Angel R. Pardillo , Kathleen Mae A. Auxtero , Kristina P. Badec , Daryl Anne B. Varela
This review examines the integration of climate-smart aquaculture (CSAq) as a strategy to enhance the resilience and sustainability of global aquaculture and coastal agriculture in the face of climate change. CSAq encompasses innovations such as integrated multi-trophic aquaculture (IMTA), genetic advancements, renewable energy integration, and optimized water management, all aimed at minimizing environmental impacts while maintaining productivity. As climate change introduces threats like ocean acidification, temperature fluctuations, and extreme weather events, CSAq offers adaptive solutions critical for preserving marine ecosystems, reducing greenhouse gas emissions, and sustaining food security. The review emphasizes that the successful adoption of CSAq is contingent upon supportive policies, cross-sectoral collaboration, and socio-economic considerations, including gender inclusivity and community involvement. As aquaculture's role in food security continues to grow, CSAq provides a pathway for mitigating climate impacts while promoting sustainable development. This review underscores the necessity of climate-smart approaches for building resilient food systems that can adapt to a changing climate and sustain livelihoods in vulnerable coastal regions.
{"title":"Climate-smart aquaculture: Innovations and challenges in mitigating climate change impacts on fisheries and coastal agriculture","authors":"Jaynos R. Cortes , Ian B. Benitez , Bernajocele Jalyn S. Baldoza , Charm Angel R. Pardillo , Kathleen Mae A. Auxtero , Kristina P. Badec , Daryl Anne B. Varela","doi":"10.1016/j.aaf.2025.08.009","DOIUrl":"10.1016/j.aaf.2025.08.009","url":null,"abstract":"<div><div>This review examines the integration of climate-smart aquaculture (CSAq) as a strategy to enhance the resilience and sustainability of global aquaculture and coastal agriculture in the face of climate change. CSAq encompasses innovations such as integrated multi-trophic aquaculture (IMTA), genetic advancements, renewable energy integration, and optimized water management, all aimed at minimizing environmental impacts while maintaining productivity. As climate change introduces threats like ocean acidification, temperature fluctuations, and extreme weather events, CSAq offers adaptive solutions critical for preserving marine ecosystems, reducing greenhouse gas emissions, and sustaining food security. The review emphasizes that the successful adoption of CSAq is contingent upon supportive policies, cross-sectoral collaboration, and socio-economic considerations, including gender inclusivity and community involvement. As aquaculture's role in food security continues to grow, CSAq provides a pathway for mitigating climate impacts while promoting sustainable development. This review underscores the necessity of climate-smart approaches for building resilient food systems that can adapt to a changing climate and sustain livelihoods in vulnerable coastal regions.</div></div>","PeriodicalId":36894,"journal":{"name":"Aquaculture and Fisheries","volume":"11 2","pages":"Pages 221-231"},"PeriodicalIF":0.0,"publicationDate":"2025-08-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145842298","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Global warming, as a key driver of climate change, is accelerating water temperature rise, with significant implications for aquatic ecosystems. Temperature, a critical abiotic factor, directly affects the physiology, growth, and survival of aquatic organisms. In the context of aquaculture—one of the fastest-growing food sectors—crustaceans hold high commercial value due to their production volume and global demand. However, their sensitivity to thermal fluctuations poses major challenge under climate change. This review examines the impact of elevated temperatures on growth performance, food intake, moulting, immune response, and survival of major farmed crustaceans, including shrimp, prawns, crabs, lobsters, and crayfish. Broader ecological consequences, such as disruptions in nutrient cycling and disease outbreaks, are also explored. Additionally, we evaluate mitigation strategies, including dietary modification, technological innovations, biotechnological approaches, and aquaculture insurance models. By integrating physiological, immunological, and ecological evidence, this review identifies key knowledge gaps and highlighting opportunities to improve climate resilience in crustacean aquaculture. Understanding these challenges is essential for sustaining productivity and promoting adaptive management in a warming world.
{"title":"Effects of climate change-induced temperature rise on crustacean aquaculture: A comprehensive review","authors":"Viraj Vishakha Yeshwant Daunde , Manoj Tukaram Kamble , Balasaheb Ramdas Chavan , Gargi Kashmira Rajesh Palekar , Sangharsh Himmat Tayade , Aranya Ponpornpisit , Kim D. Thompson , Seema Vijay Medhe , Nopadon Pirarat","doi":"10.1016/j.aaf.2025.08.008","DOIUrl":"10.1016/j.aaf.2025.08.008","url":null,"abstract":"<div><div>Global warming, as a key driver of climate change, is accelerating water temperature rise, with significant implications for aquatic ecosystems. Temperature, a critical abiotic factor, directly affects the physiology, growth, and survival of aquatic organisms. In the context of aquaculture—one of the fastest-growing food sectors—crustaceans hold high commercial value due to their production volume and global demand. However, their sensitivity to thermal fluctuations poses major challenge under climate change. This review examines the impact of elevated temperatures on growth performance, food intake, moulting, immune response, and survival of major farmed crustaceans, including shrimp, prawns, crabs, lobsters, and crayfish. Broader ecological consequences, such as disruptions in nutrient cycling and disease outbreaks, are also explored. Additionally, we evaluate mitigation strategies, including dietary modification, technological innovations, biotechnological approaches, and aquaculture insurance models. By integrating physiological, immunological, and ecological evidence, this review identifies key knowledge gaps and highlighting opportunities to improve climate resilience in crustacean aquaculture. Understanding these challenges is essential for sustaining productivity and promoting adaptive management in a warming world.</div></div>","PeriodicalId":36894,"journal":{"name":"Aquaculture and Fisheries","volume":"11 1","pages":"Pages 11-32"},"PeriodicalIF":0.0,"publicationDate":"2025-08-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145651864","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Investigating the resistance of rice-field fisheries to transition into rice-fish farming and pond aquaculture in Cambodia","authors":"Benter Anyango , Virakbot Hou , Hao Xu , Lihao Zhou , Somony Thay , Xugan Wu , Wenbo Zhang","doi":"10.1016/j.aaf.2025.08.001","DOIUrl":"10.1016/j.aaf.2025.08.001","url":null,"abstract":"","PeriodicalId":36894,"journal":{"name":"Aquaculture and Fisheries","volume":"11 2","pages":"Pages 384-399"},"PeriodicalIF":0.0,"publicationDate":"2025-08-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145842318","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Cadmium (Cd) is a widespread environmental pollutant known for its neurotoxic and endocrine-disrupting effects, yet its impact on early vertebrate development remains incompletely understood. This study investigated the toxicity and molecular mechanisms of Cd exposure in zebrafish embryos, a widely used model for developmental and neurotoxicological research. Zebrafish embryos were exposed to different concentrations of CdCl2 from 2 to 144 h post-fertilization (hpf) to evaluate morphological changes, gene expression alterations, and behavioral effects. Exposure to concentrations ≥1 μM resulted in morphological abnormalities, including altered head shape and malformations of the swim bladder and ear, along with increased mortality, while larval growth and hatching rates were unaffected. Gene expression analyses revealed that Cd exposure dysregulated key enzymes and transporters in the dopaminergic (DA) and serotonergic (5-HT) systems, with significant upregulation of th2 and tph2 and dose-dependent manner. Correspondingly, locomotor behavior was impaired, with reduced tactile response rescued by DA and 5-HT agonists, and increased spontaneous swimming reversed by both DA and 5-HT receptor antagonists. Moreover, Cd acted as an endocrine disruptor by upregulating brain aromatase (cyp19a1b) and estrogen receptor (ER) 1 (esr1), with evidence suggesting that part of its neurobehavioral toxicity is mediated through ER signaling. Altogether, our results suggest that Cd exposure disrupts neurochemical pathways and endocrine signaling during early development, leading to altered morphology and behavior in zebrafish embryos and larvae.
{"title":"Cadmium-induced disruption of monoaminergic signaling and motor behavior in developing zebrafish","authors":"Sugiyono , Zulvikar Syambani Ulhaq , Mitsuyo Kishida","doi":"10.1016/j.aaf.2025.08.007","DOIUrl":"10.1016/j.aaf.2025.08.007","url":null,"abstract":"<div><div>Cadmium (Cd) is a widespread environmental pollutant known for its neurotoxic and endocrine-disrupting effects, yet its impact on early vertebrate development remains incompletely understood. This study investigated the toxicity and molecular mechanisms of Cd exposure in zebrafish embryos, a widely used model for developmental and neurotoxicological research. Zebrafish embryos were exposed to different concentrations of CdCl<sub>2</sub> from 2 to 144 h post-fertilization (hpf) to evaluate morphological changes, gene expression alterations, and behavioral effects. Exposure to concentrations ≥1 μM resulted in morphological abnormalities, including altered head shape and malformations of the swim bladder and ear, along with increased mortality, while larval growth and hatching rates were unaffected. Gene expression analyses revealed that Cd exposure dysregulated key enzymes and transporters in the dopaminergic (DA) and serotonergic (5-HT) systems, with significant upregulation of <em>th2</em> and <em>tph2</em> and dose-dependent manner. Correspondingly, locomotor behavior was impaired, with reduced tactile response rescued by DA and 5-HT agonists, and increased spontaneous swimming reversed by both DA and 5-HT receptor antagonists. Moreover, Cd acted as an endocrine disruptor by upregulating brain aromatase (<em>cyp19a1b</em>) and estrogen receptor (ER) 1 (<em>esr1</em>), with evidence suggesting that part of its neurobehavioral toxicity is mediated through ER signaling. Altogether, our results suggest that Cd exposure disrupts neurochemical pathways and endocrine signaling during early development, leading to altered morphology and behavior in zebrafish embryos and larvae.</div></div>","PeriodicalId":36894,"journal":{"name":"Aquaculture and Fisheries","volume":"11 2","pages":"Pages 271-279"},"PeriodicalIF":0.0,"publicationDate":"2025-08-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145842790","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-08-23DOI: 10.1016/j.aaf.2025.08.004
Mengmeng Shi , Junping Wang , Haiying Shi , Jiahao Wang , Weiqun Lu
Corticotropin-releasing hormone (CRH) and Urotensin I (UI) are considered as key players of adaptive physiology. In vertebrates, the role of thyroid hormones (THs) in triggering metamorphosis through the thyroid hormone receptors (TRs) is well known. However, the changes in corticotropin-releasing hormone and urotensin I expression, as well as their regulation by thyroid hormones during metamorphosis remain unclear. In this study, we investigated the dynamic changes of T3 and cortisol levels, as well as the expression patterns of CRH, UI and TRs, and their response to T3 and a synthetic inhibitor of T3 during metamorphosis of P. olivaceus. Our results showed that CRH and UI mRNA expression levels remained low in the head during metamorphosis but sharply increased to a maximum after metamorphosis. Additionally, TRαA, TRαB, and TRβ showed distinct, stage-specific expression peaks. T3 levels peaked earlier than cortisol during metamorphosis, and exposure of 22 days after hatching (DAH) larvae to exogenous T3 significantly upregulated CRH and UI mRNA expression levels, while thiourea (TU) treatment led to their downregulation. Correlation analysis results further revealed strong positive associations among CRH, UI, and TRs under T3 treatment, especially between TRαA and TRβ at 8 h post-treatment in the 50 nM T3 group. These findings suggest that T3 may exert a stimulatory effect on CRH and UI expression during metamorphosis of P. olivaceus, potentially through TRαA and TRβ, thereby providing new insights into the endocrine regulation of metamorphosis in teleosts.
{"title":"Dynamic expression and regulation of corticotropin-releasing hormone and urotensin I during metamorphosis of olive flounder Paralichthys olivaceus","authors":"Mengmeng Shi , Junping Wang , Haiying Shi , Jiahao Wang , Weiqun Lu","doi":"10.1016/j.aaf.2025.08.004","DOIUrl":"10.1016/j.aaf.2025.08.004","url":null,"abstract":"<div><div>Corticotropin-releasing hormone (CRH) and Urotensin I (UI) are considered as key players of adaptive physiology. In vertebrates, the role of thyroid hormones (THs) in triggering metamorphosis through the thyroid hormone receptors (TRs) is well known. However, the changes in corticotropin-releasing hormone and urotensin I expression, as well as their regulation by thyroid hormones during metamorphosis remain unclear. In this study, we investigated the dynamic changes of T<sub>3</sub> and cortisol levels, as well as the expression patterns of CRH, UI and TRs, and their response to T<sub>3</sub> and a synthetic inhibitor of T<sub>3</sub> during metamorphosis of <em>P</em>. <em>olivaceus</em>. Our results showed that CRH and UI mRNA expression levels remained low in the head during metamorphosis but sharply increased to a maximum after metamorphosis. Additionally, TRαA, TRαB, and TRβ showed distinct, stage-specific expression peaks. T<sub>3</sub> levels peaked earlier than cortisol during metamorphosis, and exposure of 22 days after hatching (DAH) larvae to exogenous T<sub>3</sub> significantly upregulated CRH and UI mRNA expression levels, while thiourea (TU) treatment led to their downregulation. Correlation analysis results further revealed strong positive associations among CRH, UI, and TRs under T<sub>3</sub> treatment, especially between TRαA and TRβ at 8 h post-treatment in the 50 nM T<sub>3</sub> group. These findings suggest that T<sub>3</sub> may exert a stimulatory effect on CRH and UI expression during metamorphosis of <em>P</em>. <em>olivaceus</em>, potentially through TRαA and TRβ, thereby providing new insights into the endocrine regulation of metamorphosis in teleosts.</div></div>","PeriodicalId":36894,"journal":{"name":"Aquaculture and Fisheries","volume":"11 2","pages":"Pages 298-309"},"PeriodicalIF":0.0,"publicationDate":"2025-08-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145842793","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-08-22DOI: 10.1016/j.aaf.2025.08.002
Zheyan Chen , Xianji Tao , Xilei Li , Yihai Qiao , Xiao Cao , Changjun Mou , Jiale Li , Jianbin Feng
<div><div>Meat yield is a complex and economically significant trait for the red swamp crayfish <em>Procambarus clarkii</em>. Identifying the most suitable weight range for market readiness and establishing an integrated machine learning model to predict the meat yield addresses widespread challenges in <em>P</em>. <em>clarkii</em>, holding economic value and practical importance for the breeding of high-yield <em>P</em>. <em>clarkii</em>. Phenotypic traits including full length (FL), body length (BL), abdominal length (AL), the second uromere length (2UL), width (2UW), and height (2UH), along with body weight (BW), were measured from 1392 <em>P. clarkii</em> collected from cultivated paddy fields. Meat yield (MY) was calculated through dissection and weighing of tail meat weight (TMW). To assess the sexual dimorphism, inter-trait correlations, and multicollinearity, multiple descriptive statistical tests were employed on the collected dataset, including the data normalization, Pearson correlation coefficient calculation, and Variance Inflation Factor (VIF) calculations. To determine the optimal weight which optimizes MY and TMW across both sexes Lowess bivariate curves were utilized for fitting. various machine learning methods were utilized to predict the meat yield of <em>P. clarkii</em>, evaluated against an independent test dataset. Additionally, to understand how key traits contribute to the predictive probability of <em>P. clarkii</em> meat yield, SHapley Additive exPlanations (SHAP) interpretability methods were employed on the most accurate predictive algorithm. The AL, 2UW, 2UH, BW, and MY were statistically significant differences between sexes. FL, BL, AL, 2UL, 2UW, 2UH, BW, TMW were all highly positively correlated with each other, while MY was negatively correlated with these traits. The VIF test showed a high degree of collinearity among BL, FL, BW, and TMW. The optimal weight for maximum meat yield and tail meat was identified as 27.75 g for females and 17.51g for males. Across three machine learning models we conducted, the Gradient Boosting Regressor (GBR) model consistently produced the best predictions for meat yield in both sexes with. SHAP analysis revealed that the most critical trait examined was BW, with an explanation percentage of 40.8% in females and 46.6% in males. For females, FL (18.3%), 2UW (10.9%), and BL (10.7%) explained a high proportion among the traits included, whereas for males, BL (24.0%), AL (11.7%) and 2UH (6.6%) were more pronounced. In this study, an robust machine learning framework for accurately predicting <em>P. clarkii</em> meat yield was developed based on complex phenotypic traits. Compared to traditional destructive measurement methods and conventional regression models, this approach significantly enhances both operational efficiency and predictive accuracy. Additionally, the optimized weight threshold offers valuable insights into the commercial <em>P. clarkii</em> breeding industry, ensuring
{"title":"Meat yield prediction of the red swamp crayfish Procambarus clarkii based on machine learning","authors":"Zheyan Chen , Xianji Tao , Xilei Li , Yihai Qiao , Xiao Cao , Changjun Mou , Jiale Li , Jianbin Feng","doi":"10.1016/j.aaf.2025.08.002","DOIUrl":"10.1016/j.aaf.2025.08.002","url":null,"abstract":"<div><div>Meat yield is a complex and economically significant trait for the red swamp crayfish <em>Procambarus clarkii</em>. Identifying the most suitable weight range for market readiness and establishing an integrated machine learning model to predict the meat yield addresses widespread challenges in <em>P</em>. <em>clarkii</em>, holding economic value and practical importance for the breeding of high-yield <em>P</em>. <em>clarkii</em>. Phenotypic traits including full length (FL), body length (BL), abdominal length (AL), the second uromere length (2UL), width (2UW), and height (2UH), along with body weight (BW), were measured from 1392 <em>P. clarkii</em> collected from cultivated paddy fields. Meat yield (MY) was calculated through dissection and weighing of tail meat weight (TMW). To assess the sexual dimorphism, inter-trait correlations, and multicollinearity, multiple descriptive statistical tests were employed on the collected dataset, including the data normalization, Pearson correlation coefficient calculation, and Variance Inflation Factor (VIF) calculations. To determine the optimal weight which optimizes MY and TMW across both sexes Lowess bivariate curves were utilized for fitting. various machine learning methods were utilized to predict the meat yield of <em>P. clarkii</em>, evaluated against an independent test dataset. Additionally, to understand how key traits contribute to the predictive probability of <em>P. clarkii</em> meat yield, SHapley Additive exPlanations (SHAP) interpretability methods were employed on the most accurate predictive algorithm. The AL, 2UW, 2UH, BW, and MY were statistically significant differences between sexes. FL, BL, AL, 2UL, 2UW, 2UH, BW, TMW were all highly positively correlated with each other, while MY was negatively correlated with these traits. The VIF test showed a high degree of collinearity among BL, FL, BW, and TMW. The optimal weight for maximum meat yield and tail meat was identified as 27.75 g for females and 17.51g for males. Across three machine learning models we conducted, the Gradient Boosting Regressor (GBR) model consistently produced the best predictions for meat yield in both sexes with. SHAP analysis revealed that the most critical trait examined was BW, with an explanation percentage of 40.8% in females and 46.6% in males. For females, FL (18.3%), 2UW (10.9%), and BL (10.7%) explained a high proportion among the traits included, whereas for males, BL (24.0%), AL (11.7%) and 2UH (6.6%) were more pronounced. In this study, an robust machine learning framework for accurately predicting <em>P. clarkii</em> meat yield was developed based on complex phenotypic traits. Compared to traditional destructive measurement methods and conventional regression models, this approach significantly enhances both operational efficiency and predictive accuracy. Additionally, the optimized weight threshold offers valuable insights into the commercial <em>P. clarkii</em> breeding industry, ensuring","PeriodicalId":36894,"journal":{"name":"Aquaculture and Fisheries","volume":"11 2","pages":"Pages 333-340"},"PeriodicalIF":0.0,"publicationDate":"2025-08-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145842293","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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