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cDNA-AFLP-based transcript profiling for the identification of genes functionally related to growth in the pond-reared giant tiger shrimp Penaeus monodon 基于 cDNA-AFLP 的转录本分析鉴定池塘饲养的巨型虎虾中与生长功能相关的基因
Q1 Agricultural and Biological Sciences Pub Date : 2025-07-01 DOI: 10.1016/j.aaf.2024.04.011
Rachanimuk Hiransuchalert , Apirak Chaimeanwong , Patchari Yocawibun , Parichat Chumtong , Natechanok Thamniemdee , Walailuk Thungkoon , Vasin Yuvanatemiya , Bavornlak Khamnamtong , Sirawut Klinbunga
Genes exhibiting differential expression patterns between large-sized (average body weight 17.57 ± 1.8 g and 40.97 ± 4.0 g for 3- and 5-month-old juveniles in a total of 342 and 356 samples, respectively) and small-sized (average body weight 10.01 ± 0.76 g and 22.86 ± 2.51 g, respectively) juvenile giant tiger shrimps (Penaeus monodon) were isolated by complementary DNA-amplified fragment length polymorphism (cDNA-AFLP). In total, 368 primer combinations were screened against the first-strand cDNA of the different groups of 3- and 5-month-old P. monodon.
Over 146 differentially expressed or size-specific markers were derived using 59 selective primer combinations. A total of 70 candidate cDNA-AFLP markers—19 size-specific and 51 showing differential expression—were further cloned, sequenced, and blasted against the GenBank database. Twenty-five markers were found to comprise newly identified sequences (E-values >10−4).
Quantitative real-time PCR and Single-strand conformational polymorphism (SSCP) analyses of the products generated with the SCAR/cDNA-AFLP primers were further undertaken. Interestingly, the relative expression levels of the products of primers E+34M+314-550-PDI, E+35M+310-350-Unk, E+29/M+211-350-CHK, E+34/M+31-380-phosphotysosyl, and E+38/M+315-800-fucosidase were significantly upregulated in the hepatopancreas of large-sized juvenile shrimp compared with that is small-sized animals. In contrast, the relative expression levels of the products generated by E+31M+38-480-InositolOxy and E+29/M+211-330-vitelline membrane were significantly upregulated in small-sized P. monodon.
The consistency of our results was further assessed in 1-month-old aquaculture-raised P. monodon. Single nucleotide polymorphisms were identified using SSCP at different positions in the sequences of fragments of the different groups generated with the primers E+34M+314-550-PDI and E+35M+310-350-Unk. Our results indicated that cDNA-AFLP has the potential for use in the isolation of functionally important transcripts in P. monodon.
利用互补dna扩增片段长度多态性(cDNA-AFLP)技术,分离出大、小对虾幼虾(342份和356份,3月龄和5月龄,平均体重分别为17.57±1.8 g和40.97±4.0 g)和小对虾幼虾(平均体重分别为10.01±0.76 g和22.86±2.51 g)的差异表达基因。共筛选了368个引物组合,分别针对3月龄和5月龄单毛单胞虫不同群体的第一链cDNA。使用59种选择性引物组合获得了146种差异表达或大小特异性标记。共有70个候选cDNA-AFLP标记(19个大小特异性和51个差异表达)被进一步克隆、测序,并与GenBank数据库进行比对。发现25个标记包含新鉴定的序列(e值>;10−4)。进一步对SCAR/cDNA-AFLP引物生成的产物进行实时定量PCR和单链构象多态性(SSCP)分析。有趣的是,引物E+34M+314-550-PDI、E+35M+310-350-Unk、E+29/M+ 311 -350- chk、E+34/M+31-380-phosphotysosyl、E+38/M+315-800- focusidase在大型对虾幼虾肝胰腺中的相对表达量较小型动物显著上调。相比之下,E+31M+38-480-InositolOxy和E+29/M+211-330-卵黄膜产生的产物在小型单齿藻中相对表达量显著上调。在养殖1月龄的单斑绦虫中进一步评估了结果的一致性。引物E+34M+314-550-PDI和E+35M+310-350-Unk生成的不同类群的片段序列不同位置,采用SSCP方法鉴定了单核苷酸多态性。我们的结果表明,cDNA-AFLP有潜力用于分离单殖单胞虫的功能重要转录本。
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引用次数: 0
Quality-based selection of the optimal hot air gradient drying method for anchovy and modeling of drying kinetics 基于质量的凤尾鱼最佳热空气梯度干燥方法选择和干燥动力学建模
Q1 Agricultural and Biological Sciences Pub Date : 2025-07-01 DOI: 10.1016/j.aaf.2024.03.002
Yiting Ye , Ting Zhou , Teyuan Liu , Wenzheng Shi
The aim of this study was to investigate the effects of hot air gradient drying on anchovy drying quality and efficiency. Anchovies were initially dried at 40 °C to until their moisture content was 40%, 45%, 50%, 55%, 60%, and 65% respectively, followed by a second drying stage at an increased temperature of 60 °C until the moisture content reached 40%. Six drying models were selected to fit the anchovy drying curves, and the Page model was found to be conformed the anchovy drying model. The drying time decreased by a minimum of 8.3% and a maximum of 33.3% as the moisture content at the turning point rose. In addition, the rehydration rate of dried anchovies increased and then decreased with increasing moisture content, while the TBA and TVBN values decreased and then increased, and there were differences in the textural properties of drying at different moisture contents. Among them, the dried anchovies with a moisture content of 55% at the turning point had the highest rehydration ratio, the lowest TBA and TVBN values, and better textural characteristics.
本研究旨在探讨热风梯度干燥对鳀鱼干燥品质和效率的影响。首先在40°C干燥凤尾鱼,使其含水量分别为40%、45%、50%、55%、60%和65%,然后在60°C的温度下进行第二次干燥,使其含水量达到40%。选取6种干燥模型对鳀鱼的干燥曲线进行拟合,发现Page模型与鳀鱼的干燥模型吻合。随着拐点含水率的升高,干燥时间最小减少8.3%,最大减少33.3%。此外,随含水量的增加,凤尾鱼干的复水化率先升高后降低,TBA值和TVBN值先降低后升高,不同含水量下的干燥结构特性存在差异。其中,拐点水分含量为55%的凤尾鱼干复水化率最高,TBA和TVBN值最低,质地特性较好。
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引用次数: 0
Reusing biofloc-culture water and microbubble aeration for culturing the white-leg shrimp Litopenaeus vannamei 重复使用生物絮凝培养水和微泡曝气法培养南美白对虾
Q1 Agricultural and Biological Sciences Pub Date : 2025-07-01 DOI: 10.1016/j.aaf.2024.04.010
Ezzah Rahimah Samsuri , Poobalan Ganesan , Iswadi Jauhari , Geok Yuan Annie Tan , Faik A. Hamad
This study investigated the effects of reusing biofloc-culture water for culturing the white-leg shrimp Litopenaeus vannamei. Experiments were conducted for two continuous cycles without water exchange for 30 days during the nursery stage. The studies were performed with two different aeration systems, namely microbubbles (MB) and large air bubbles (LB). Water quality parameters such as dissolved oxygen (DO), temperature, pH, salinity, alkalinity, total ammonia nitrogen (TAN), nitrite, nitrate, settleable solids (SS), and total suspended solids (TSS) were recorded throughout the culture days. Also, comparisons were performed on parameters of the shrimp growth, i.e., body weight and length, feed conversion ratio (FCR), and specific growth rates (SGR) for the 30-day culture for Cycles 1 and 2. Cycle 2 showed no deterioration in water quality and no significant difference in growth performance compared to Cycle 1 for either aeration method (MB and LB). However, when comparing between aeration methods, MB aeration significantly improved the DO of the water and accelerated the conversion of TAN to nitrate. Shrimps in the MB aeration system were significantly larger (by 39%) with a lower FCR value (reduced by 30%) than in the LB aeration system. Harmful bacteria from the Vibro group were detected. However, they occurred at a minimum level in both cycles and aeration types. Overall, the biofloc-culture water might be reused for the subsequent cycle, while the MB aeration might promote the growth of biofloc and L. vannamei.
本试验研究了重复利用生物絮凝水体养殖凡纳滨对虾的效果。苗期连续2个周期不换水30 d。研究采用两种不同的曝气系统,即微气泡(MB)和大气泡(LB)。在整个培养过程中记录溶解氧(DO)、温度、pH、盐度、碱度、总氨氮(TAN)、亚硝酸盐、硝酸盐、可沉降固形物(SS)和总悬浮固形物(TSS)等水质参数。并比较了第1和第2周期30 d的对虾生长参数,即体重和体长、饲料系数(FCR)和特定生长率(SGR)。两种曝气方式(MB和LB)在循环2中均未出现水质恶化,生长性能与循环1无显著差异。然而,在不同曝气方式的对比中,MB曝气显著提高了水体的DO,加速了TAN向硝酸盐的转化。与LB曝气系统相比,MB曝气系统对虾体型显著增大(增加39%),FCR值显著降低(减少30%)。检测了Vibro组的有害细菌。然而,在循环和曝气类型中,它们都发生在最低水平。综上所述,生物絮团培养水可在后续循环中重复使用,而MB曝气可促进生物絮团和凡纳梅的生长。
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引用次数: 0
Effects of dietary Pediocuccus pentosaceus and/or ferulic acid on performance, digestive enzymes, blood biochemistry, and growth-related genes expression in rainbow trout (Oncorhynchus mykiss) 膳食五味子和/或阿魏酸对虹鳟鱼(Oncorhynchus mykiss)生长性能、消化酶、血液生化和生长相关基因表达的影响
Q1 Agricultural and Biological Sciences Pub Date : 2025-07-01 DOI: 10.1016/j.aaf.2024.06.005
Majid Habibnia , Somayeh Bahram , Masoumeh Bahrekazemi , Seyed Rohollah Javadian , Masoud Hedayatifard , Mohsen Abdel-Tawwab
This study investigated the effects of dietary Pediocuccus pentosaceus (PP) and/or ferulic acid (FA) on the performance, digestive enzymes, and growth-related genes expression in rainbow trout (Oncorhynchus mykiss). Fish (94.30 ± 1.77 g) were fed on the control diet (T0), 108 CFU/g of PP probiotic (T1), 100 mg/kg of FA (T2), and a combination of PP and FA (T3) for 8 weeks. The present results indicated that rainbow trout fed with PP and/or FA showed better final weight, weight gain, and feed conversion ratio than the control group (P < 0.05). The activities of trypsin and chymotrypsin were higher in T2 and T3 than in the control group (P < 0.05). Meanwhile activities protease and alpha-amylase were highest in T2, lipase activity was highest in T3. Compared to the control group, total protein, total antioxidant activity, and cholesterol levels were significantly (P < 0.05) higher in T2 and T3 with no significant (P > 0.05) differences between them. Supplemented diets with FA and/or PP decreased markedly (P < 0.05) triglycerides levels and its lowest levels were obtained in T2 and T3 with no significant (P > 0.05) differences between them. The relative expression levels of GHRL and GHr genes were significantly higher in the treatments fed with FA and/or PP (T1 – T3) than those in the control group (P < 0.05). Highest upregulation of IGF-1, and IGF-II gens were detected in T2 and T3 with no significant (P > 0.05) differences between them. In conclusion, it was shown that both dietary supplements have the potential role to enhance the performance, digestive enzymes, and growth related-genes expression in rainbow trout. Feeding fish on FA-enriched diets showed superior enhancements than that fed on the PP diet only.
本试验研究了饲粮中添加戊saceus (Pediocuccus pentosaceus, PP)和(或)阿威酸(ferulic acid, FA)对虹鳟生产性能、消化酶和生长相关基因表达的影响。试验饲喂对照饲料(T0)、108 CFU/g PP益生菌(T1)、100 mg/kg FA (T2)和PP + FA (T3)混合饲料(94.30±1.77 g) 8周。本研究结果表明,添加聚丙烯和/或FA的虹鳟鱼的最终体重、增重和饲料系数均优于对照组(P <;0.05)。T2和T3组胰蛋白酶和凝乳胰蛋白酶活性均高于对照组(P <;0.05)。蛋白酶和α -淀粉酶活性在T2时最高,脂肪酶活性在T3时最高。与对照组相比,总蛋白、总抗氧化活性和胆固醇水平显著降低(P <;(P > 0.05)增高,但无统计学意义(P >;它们之间的差异为0.05)。饲粮中添加FA和/或PP显著降低(P <;T2和T3时甘油三酯水平和最低水平均无显著差异(P >;它们之间的差异为0.05)。饲粮中添加FA和/或PP (T1 - T3)组GHRL和GHr基因的相对表达量显著高于对照组(P <;0.05)。IGF-1和IGF-II基因在T2和T3时上调最高,但无显著差异(P >;它们之间的差异为0.05)。综上所述,这两种膳食补充剂都有提高虹鳟鱼生产性能、消化酶和生长相关基因表达的潜在作用。饲喂富含fa饲料的鱼比只饲喂PP饲料的鱼表现出更好的增强效果。
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引用次数: 0
Characterization of a new strain (SR-11) with thin blades and fast growth in Pyropia haitanensis (Bangiales, Rhodophyta) 具有薄叶片和快速生长特性的 Pyropia haitanensis(红藻纲,Bangiales, Rhodophyta)新菌株(SR-11)的特征描述
Q1 Agricultural and Biological Sciences Pub Date : 2025-07-01 DOI: 10.1016/j.aaf.2024.05.004
Min Tian , Hongchang Ding , Xinghong Yan
A new strain (SR-11) was selected from an intraspecific hybridization of a new cultivar (SF-2, ♀) and an improved strain (HR-6, ♂) of Pyropia haitanensis, and the characteristics of blades thickness, growth rate, phycobiliprotein content, and differentiation rate of conchocelis of SR-11 were analyzed through laboratory culture. After being cultured for 45 days, the mean thickness of SR-11 was 22.9 μm, which was 37.1% and 18.5% thinner than that of SF-2 and HR-6, respectively; the phycobiliprotein of SR-11 was 94.6 mg/g, increased by 4.1% and decreased by 8.4% compared with that of SF-2 and HR-6, respectively. After being cultured for 55 days, the mean length of the SR-11 was 293.7 cm, which was 3.1 and 7.2 times longer than that of SF-2 and HR-6, respectively. After the conchocelis inoculated shell were raised the temperature and shrunk of light time, the time for the emergence of a lot of conchosporangial branches in SR-11 was shorter than that of SF-2. The total number of conchospores released from per shell in SR-11 was 144.5 × 104, which was 78.1% and 11.9% higher than that of SF-2 and HR-6, respectively. In summary, the SR-11 has thinner blades, and its fresh blades may be further processed into higher value-added products. Besides, it has the advantages of fast growth, high quality and large number of conchospores released, which is expected to be applied in production cultivation.
对海檀焦斑(Pyropia haitanensis)新品种(SF-2,♀)和改良菌株(HR-6,♂)进行种内杂交,筛选出一株新菌株SR-11,并通过室内培养分析了SR-11的叶片厚度、生长速率、藻胆蛋白含量和螺珠分化率等特征。培养45 d后,SR-11的平均厚度为22.9 μm,分别比SF-2和HR-6薄37.1%和18.5%;SR-11的藻胆蛋白为94.6 mg/g,比SF-2和HR-6分别提高了4.1%和8.4%。培养55 d后,SR-11的平均长度为293.7 cm,分别是SF-2和HR-6的3.1倍和7.2倍。在提高接种螺壳的温度和缩短光照时间后,SR-11的螺孢子囊分支出现的时间比SF-2短。SR-11单壳释放螺孢子总数为144.5 × 104个,分别比SF-2和HR-6高78.1%和11.9%。综上所述,SR-11的叶片更薄,其新鲜叶片可以进一步加工成更高附加值的产品。此外,它还具有生长快、质量好、释放螺孢子量大等优点,有望在生产栽培中得到应用。
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引用次数: 0
Corrigendum regarding missing ethics statement in previously published articles 关于先前发表的文章中缺少伦理声明的更正
Q1 Agricultural and Biological Sciences Pub Date : 2025-07-01 DOI: 10.1016/j.aaf.2025.01.005
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引用次数: 0
Dietary supplementation of Withania somnifera and Asparagus racemosus enhances growth performance coupled with transcriptional modulation of growth and immune responsive genes in fish, Channa punctatus 饲粮中添加藜草和总状芦笋可通过转录调控马尾松鱼的生长和免疫应答基因提高其生长性能
Q1 Agricultural and Biological Sciences Pub Date : 2025-07-01 DOI: 10.1016/j.aaf.2024.03.007
Sunil P. Trivedi , Shikha Dwivedi , Shefalee Singh , Adeel Ahmad Khan , Manoj Kumar , Indrani Dubey , Abha Trivedi , Vivek Kumar , Kamlesh K. Yadav
The growth amelioration potential of two herbal feed supplements, Withania somnifera and Asparagus racemosus, individually and in combination, was evaluated up to 60 days of feeding trial in a teleost, Channa punctatus. 15 days laboratory acclimatized fish (14 ± 1) g; (11 ± 1) cm were randomly distributed in 10 groups- C (control), S1, S2, S3, A1, A2, A3, AS1, AS2, and AS3, based on the composition of the dietary supplements. Each group had 10 specimens (n = 10), and the experiment was performed in triplicate. Elevated final body weight (FBW) was observed in all groups except S1 after 60 days, and weight gain percentage (WG %) showed a significant increase in all groups except S1 after both 30 and 60 days of the sampling periods with a significance level of P < 0.05. In contrast, the feed conversion ratio (FCR) significantly (P < 0.05) decreased in all experimental groups, except S1, as compared to the control, after 60 days of sampling duration. The hepatosomatic index (HSI %) significantly (P < 0.05) increased in groups - S3, A2, A3, AS1, AS2, and AS3; while the viscerosomatic index (VSI %) also significantly (P < 0.05) enhanced in groups - A3, AS1, AS2, and AS3 as compared to C. Significant (P < 0.05) elevations in activities of both trypsin and chymotrypsin, after both the sampling durations were recorded; the maximum being in AS3 as 3.47 and 2.06-fold, respectively, after 60 days, suggests greater protein digestibility. The qRT-PCR analysis displays an up-regulation of growth-related genes-gh, igf-1, and igf-2a, both in the liver and muscle tissues. Also, gh showed greater expression at transcriptional levels in liver tissues (4.86)-fold than muscle tissues (4.43)-fold in AS3; whereas igf-1 and igf-2a were significantly (P < 0.05) upregulated in the muscles of AS2 and AS3 as compared to C. Further, significantly (P < 0.05) increased expression of the transforming growth factor-β (tgf-β) in liver tissues was recorded in groups- AS1, AS2, and AS3, as compared to C, suggesting the immunoprotective role of combined dietary supplements. The study envisages that 3% dietary supplementation with both herbs (AS3) sufficiently influences the activities of digestive enzymes, growth indices, and mRNA levels of growth-related and immunoprotective genes in the fish. The study thus finds ample scope in aquaculture.
在长达60天的试验中,对两种中草药饲料添加物——粗花菊和总状芦笋单独和组合对硬骨鱼马尾鱼生长的改善潜力进行了评估。15天实验室驯化鱼(14±1)g;(11±1)cm,根据饲粮添加剂的组成,随机分为C(对照组)、S1、S2、S3、A1、A2、A3、AS1、AS2和AS3组。每组10只(n = 10),实验一组重复。60 d后,除S1组外,其余各组的末重均显著升高;30 d和60 d后,除S1组外,其余各组的增重率均显著升高(P <显著水平);0.05. 相比之下,饲料系数(FCR)显著(P <;除S1外,各试验组在60 d后均较对照组降低。肝体指数(HSI %)显著降低(P <;0.05), S3、A2、A3、AS1、AS2、AS3组均升高;而脏器指数(VSI %)也显著(P <;0.05), A3组、AS1组、AS2组和AS3组与c组比较显著(P <;0.05)胰蛋白酶和凝乳胰蛋白酶活性的升高,在两个采样时间后进行记录;AS3期最高,60 d后分别为3.47倍和2.06倍,说明蛋白质消化率较高。qRT-PCR分析显示,肝脏和肌肉组织中生长相关基因gh、igf-1和igf-2a均上调。此外,在AS3中,gh在肝脏组织的转录水平表达(4.86)倍高于肌肉组织(4.43)倍;而igf-1和igf-2a显著(P <;0.05)在AS2和AS3肌肉中表达上调,进一步,显著(P <;0.05),与C组相比,AS1、AS2和AS3组肝脏组织中转化生长因子-β (tgf-β)表达增加,提示联合膳食补充具有免疫保护作用。该研究设想,在饲料中添加3%的这两种草药(AS3)足以影响鱼的消化酶活性、生长指数以及生长相关基因和免疫保护基因的mRNA水平。因此,该研究在水产养殖中发现了充足的应用空间。
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引用次数: 0
Corrigendum regarding missing ethics statement, consent to participate statements and declaration of competing interest in articles previously published in Volume 3 关于先前在第3卷中发表的文章中缺失的伦理声明、同意参与声明和竞争利益声明的更正
Q1 Agricultural and Biological Sciences Pub Date : 2025-07-01 DOI: 10.1016/j.aaf.2024.11.008
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引用次数: 0
Morphology and structure of scales at the lateral-line region of Hyporhamphus limbatus in the Vietnamese Mekong Delta 越南湄公河三角洲边缘小鳞(Hyporhamphus limbatus)侧线区鳞片形态与结构
Q1 Agricultural and Biological Sciences Pub Date : 2025-06-27 DOI: 10.1016/j.aaf.2025.06.001
Vinh Quoc Nguyen, Vuong Van Ly, Phuc Le Hoang Nguyen, Ly Thi Cam Tran, Quyet Le Nguyen, Quang Minh Dinh
This study examines the morphology and structure of scales at the lateral-line region of Hyporhamphus limbatus, specifically the lateral-line scales (lls), dorsal-to lateral-line scales (dol), and ventral-to lateral-line scales (vel), based on 100 specimens collected from Phuoc Long - Bac Lieu and Dam Doi - Ca Mau between July and September 2024. After identifying the scales, their dimensions and fish total length were measured to investigate the regression relationship between these variables. The scales were subsequently dyed to observe and analyze their morphology and structure in greater detail. The results showed that Hyporhamphus limbatus had 40 to 69 lls (51 ± 5 SE), with a mean length of 0.51 ± 0.04 SE mm and width of 0.30 ± 0.01 SE mm. The species exhibited 36 to 52 dol (46 ± 6 SE scales), with an average radius of 2.09 ± 0.06 SE mm, and 41 to 57 vel (49 ± 2 SE scales), measuring 1.83 ± 0.02 SE mm. A regression analysis between the length of the lateral-line scales and the fish total length showed a positive relationship (R2 > 0.90). This research on the morphology and structure of scales in Hyporhamphus limbatus aims to enhance understanding of sex-based growth patterns and differentiation. The findings also contribute to characterizing the fish scale diversity, providing a valuable resource for future studies on this species’ biological traits, mainly through examining its scales.
本文以2024年7月至9月在Phuoc Long - Bac Lieu和Dam Doi - Ca Mau采集的100个样本为研究对象,研究了limbatus侧线区鳞片的形态和结构,特别是侧线鳞片(lls)、背-侧线鳞片(dol)和腹-侧线鳞片(vel)。在确定鱼鳞后,测量鱼鳞的尺寸和鱼的总长度,以研究这些变量之间的回归关系。这些鳞片随后被染色,以更详细地观察和分析它们的形态和结构。结果表明,Hyporhamphus limbatus 40到69之上》(51±5),平均长度为0.51±0.04 SE的mm和宽度0.30±0.01 SE毫米。这个物种表现出36至52痛单位(46±6 SE尺度),平均半径为2.09±0.06 SE的mm, 41 57韦尔(49±2 SE尺度),测量1.83±0.02 SE毫米。段的长度之间的回归分析鳞鱼总长度显示积极的关系(R2的在0.90)。本研究旨在对边缘小鳞的形态和结构进行研究,以加深对其基于性别的生长模式和分化的认识。研究结果还有助于表征鱼鳞的多样性,为进一步研究鱼鳞的生物学特性提供了宝贵的资源。
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引用次数: 0
Decoding feed efficiency: Liver and intestinal transcriptomics in Larimichthys crocea fed a fishmeal-free diet 解码饲料效率:无鱼粉饲料喂养的大菱鲆肝脏和肠道转录组学
Q1 Agricultural and Biological Sciences Pub Date : 2025-06-19 DOI: 10.1016/j.aaf.2025.05.006
Ranxin Fu , Gongsi Wang , Xiaotong Li , Xingcheng Zhu , Peng Ren , Lining Zhang , Qinghui Ai , Zhiyong Wang
Feed efficiency (FE) is a key indicator of an animal's ability to convert feed into growth, directly impacting production costs and sustainability in aquaculture. This study aimed to explore the liver and intestinal transcriptomic profiles of Larimichthys crocea individuals with high FE (H group) and low FE (L group) under a fishmeal-free diet. Differentially expressed genes (DEGs) and associated regulatory pathways were identified to shed light on potential genetic factors influencing FE traits. Compared to the L group, 53, 24, and 112 DEGs were detected in the liver, proximal intestine, and distal intestine tissues of the H group, respectively. Notably, 24 up- and 29 down-regulated genes were found in the liver, 9 up- and 15 down-regulated genes in the proximal intestine, and 41 up- and 71 down-regulated genes in the distal intestine. GO and KEGG pathway analyses highlighted 15 key genes related to metabolism (acsl3fads2acacagpamcel、slc10a2pemtagpat3、agpat4acot1 and pla2g15) and growth (irs2fgf10、rnf15, and deptor) as potential candidates for improving FE in L. crocea. Quantitative PCR confirmed the up- or down-regulation of these genes in line with RNA-seq results. This study offers valuable insights into the molecular mechanisms underlying feed efficiency in L. crocea and identifies candidate genes for molecular breeding aimed at enhancing FE.
饲料效率(FE)是衡量动物将饲料转化为生长能力的关键指标,直接影响水产养殖的生产成本和可持续性。本研究旨在探讨无鱼粉饲料条件下高铁组(H组)和低铁组(L组)罗非鱼(Larimichthys crocea)个体肝脏和肠道转录组学特征。鉴定差异表达基因(DEGs)和相关的调控途径,以揭示影响FE性状的潜在遗传因素。与L组相比,H组肝脏、近端肠和远端肠组织中分别检测到53、24和112个deg。值得注意的是,肝脏中有24个上调基因和29个下调基因,近端肠中有9个上调基因和15个下调基因,远端肠中有41个上调基因和71个下调基因。GO和KEGG通路分析突出了15个与代谢(acsl3、fads2、acaca、gpam、cell、slc10a2、ppt、agpat3、agpat4、acot1和pla2g15)和生长(irs2、fgf10、rnf15和deptor)相关的关键基因,它们是改善L. crocea FE的潜在候选基因。定量PCR证实了这些基因的上调或下调与RNA-seq结果一致。本研究为进一步了解沼虾饲料效率的分子机制提供了有价值的见解,并为提高沼虾饲料效率的分子育种提供了候选基因。
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Aquaculture and Fisheries
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