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Evaluating the genetic diversity in farmed populations of Nile tilapia (Oreochromis niloticus) from Brazil using SNP markers 利用SNP标记评价巴西尼罗罗非鱼养殖种群的遗传多样性
Q1 Agricultural and Biological Sciences Pub Date : 2025-05-31 DOI: 10.1016/j.aaf.2025.05.004
Vito Antonio Mastrochirico-Filho , Baltasar Fernandes Garcia , Lieschen Valeria Guerra Lira , Antonio Ramon do Amaral Neto , Rodrigo Marín-Nahuelpi , Jousepth Gallardo-Hidalgo , Liane Ney Bassini , Fabio Porto-Foresti , Diogo Teruo Hashimoto
The Nile tilapia (Oreochromis niloticus) plays a significant role in global aquaculture, with Brazil ranking among the top producers worldwide. However, a considerable gap remains in the exploration of genetic diversity within Brazilian Nile tilapia stocks. To address this, we analyzed the genetic diversity of nine distinct farmed populations of Nile tilapia in Brazil, comprising a total of 600 individuals, using single-nucleotide polymorphisms (SNPs). Our objective was to provide essential genetic insights to establish the foundation for a new breeding nucleus. The pre-breeding populations exhibited high genetic diversity. Among them, Pop1 had the lowest diversity, characterized by the smallest proportion of polymorphic SNPs (NP = 92.3%), the lowest mean minor allele frequency (MAF = 0.24), and the lowest heterozygosity (HE = 0.32). In contrast, Pop5 and Pop9 displayed the highest genetic diversity (MAF = 0.30; HE = HO 0.39), with nearly all loci in Hardy-Weinberg equilibrium (HWE = 99.4%). Additionally, reduced effective population sizes (Ne) were observed in Pop1 (Ne = 6.8) and Pop4 (Ne = 15.4), with approximately 40 % of individuals classified as half-of full siblings, suggesting risks of inbreeding. These characteristics led to the genetic structuring of Pop1 and Pop4 in relation to the other populations, while Pop5 and Pop9 also formed a distinct cluster due to their higher genetic diversity. Despite the overall high genetic diversity observed across the analyzed populations, some populations exhibited parameters that may indicate potential future issues related to genetic diversity loss if not properly managed. Therefore, the findings presented here will help ensure the implementation of effective strategies during the pre-breeding phase of a new tilapia genetic improvement nucleus, supporting the long-term maintenance of genetic diversity in the breeding stock.
尼罗罗非鱼(Oreochromis niloticus)在全球水产养殖中发挥着重要作用,巴西是世界上最大的罗非鱼生产国之一。然而,在探索巴西尼罗罗非鱼种群的遗传多样性方面仍存在相当大的差距。为了解决这个问题,我们利用单核苷酸多态性(snp)分析了巴西9个不同的尼罗罗非鱼养殖种群的遗传多样性,共包括600个个体。我们的目标是提供必要的遗传见解,以建立一个新的育种核的基础。育种前群体表现出较高的遗传多样性。其中,Pop1的多样性最低,多态性snp比例最小(NP = 92.3%),平均次要等位基因频率最低(MAF = 0.24),杂合性最低(HE = 0.32)。相比之下,Pop5和Pop9的遗传多样性最高(MAF = 0.30, HE = 0.39),几乎所有位点都处于Hardy-Weinberg平衡(HWE = 99.4%)。此外,在Pop1 (Ne = 6.8)和Pop4 (Ne = 15.4)中观察到有效种群大小(Ne)减小,大约40%的个体被归类为半全兄弟姐妹,提示近亲繁殖的风险。这些特征导致Pop1和Pop4的遗传结构相对于其他居群,而Pop5和Pop9也因其较高的遗传多样性而形成了一个明显的集群。尽管在分析的种群中观察到总体上较高的遗传多样性,但一些种群表现出的参数可能表明,如果管理不当,未来可能会出现与遗传多样性丧失有关的潜在问题。因此,本文提出的研究结果将有助于确保在新的罗非鱼遗传改良核的育种前阶段实施有效的策略,支持长期维持种畜的遗传多样性。
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引用次数: 0
Genetic diversity and population structure of different red swamp crayfish (Procambarus clarkii) populations across the north and south of China 中国南北不同沼泽红螯虾(原螯虾)种群的遗传多样性和种群结构
Q1 Agricultural and Biological Sciences Pub Date : 2025-05-31 DOI: 10.1016/j.aaf.2025.04.004
Qing Mu , Chuntong Liu , Zhiyan Wang , Qizhou Xu , Liang Jia , Zhiyi Bai
The red swamp crayfish, Procambarus clarkii, has become one of the most important freshwater aquaculture species in China. The crayfish culture extends from the middle and lower areas of Yangtze River to the northern and southern part of China in recent ten years. To understand genetic variability during geographical adaption, we performed whole genome re-sequencing (WGS) of six crayfish populations across the north and south of China: Panjin (PJ), Liaoning province; Weishan (WS), Shandong province; Wuhu (WH), Anhui province; Jianhu (JH), Jiangsu province; Gehu (GH), Jiangsu province; Haikou (HK), Hainan province. A total of 120,906,91 high-quality single nucleotide polymorphisms (SNPs) were identified across the crayfish genome. The six geographical populations showed high level of diversity with the expected heterozygosity (He) ranged from 0.291 (WS) to 0.314 (PJ). The principal component and neighbor-joining (NJ) phylogeny analysis categorized the 180 individuals into three subgroups: the PJ and WS populations formed two separate subgroups, while the GH, JH, WH, and HK populations converged into a single subgroup. Moreover, the pairwise comparison of fixation index (Fst) values indicated a minor to moderate level of genetic differentiation among these populations, ranging from 0.0037 (GH, JH, WH & HK) to 0.0717 (WS & PJ), suggesting a greater genetic distance between the WS population and PJ population. Taken together, the SNPs identified in this study can serve as valuable genetic markers in future germplasm improvement of P. clarkii cultured stocks. These results will provide genetic information for further germplasm conservation and genetic improvement of P. clarkii.
克氏原螯虾(Procambarus clarkii)已成为中国最重要的淡水养殖品种之一。近十年来,小龙虾养殖从长江中下游地区向中国北部和南部地区扩展。为了了解地理适应过程中的遗传变异,我们对中国北部和南部的6个小龙虾种群进行了全基因组重测序(WGS):辽宁盘锦;山东微山(WS);安徽芜湖;江苏建湖;江苏葛湖(GH);海口(香港),海南省。在整个小龙虾基因组中共鉴定出120,906,91个高质量的单核苷酸多态性(snp)。6个地理居群的期望杂合度(He)在0.291 (WS) ~ 0.314 (PJ)之间,具有较高的多样性。主成分和邻联(NJ)系统发育分析将180个个体划分为3个亚群:PJ和WS居群为两个独立的亚群,GH、JH、WH和HK居群为一个亚群。此外,固定指数(Fst)两两比较表明,这些群体之间的遗传分化程度为轻微至中等,范围为0.0037 (GH, JH, WH & HK)至0.0717 (WS &; PJ),表明WS与PJ群体之间存在较大的遗传距离。综上所述,本研究所鉴定的snp可作为未来克氏假单胞菌种质改良中有价值的遗传标记。这些结果将为进一步的克氏假单胞菌种质资源保护和遗传改良提供遗传信息。
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引用次数: 0
Combined supplementation of hydroxyproline and vitamin C improved the growth and flesh quality of Pacific white shrimp (Litopenaeus vanname) cultured in low salinity water 联合补充羟脯氨酸和维生素 C 可改善低盐度水域养殖的太平洋南美白对虾的生长和肉质
Q1 Agricultural and Biological Sciences Pub Date : 2025-05-01 DOI: 10.1016/j.aaf.2023.12.007
Tianyu Huang , Beibei Guo , Jinyu Zheng , Menglu Li , Yunfeng Chen , Xiaoqin Li , Xiangjun Leng
This study investigated the effects of hydroxyproline (Hyp) and vitamin C (VC) addition to practical diet on growth and flesh quality of Pacific white shrimp (Litopenaeus vannamei) cultured in low salinity water. The control diet was formulated to contain 200 g/kg fish meal (FM20), and then 14 g/kg coated hydroxyproline (50%) (FM20H), 2 g/kg L-ascorbyl-2-phosphate (38%) (FM20C) and their combination (FM20HC) were added to the control diet to form four isoproteic (41.6%) and isolipidic (6.7%) diets. Pacific white shrimp with initial body weight of (1.50 ± 0.05) g were fed the above four diets in low salinity water (salinity 1‰) for 8 weeks. The results showed that the combined supplementation of Hyp and VC (FM20HC) increased the weight gain by 13.4%, and decreased the feed conversion ratio by 0.18 (P < 0.05), while the individual supplementation (FM20H and FM20C groups) just numerically promoted the growth (P > 0.05). In addition, the FM20HC group also showed higher protein and lipid retention than the FM20 group (P < 0.05). In flesh quality, the supplementation of Hyp (FM20H) increased total collagen and heat-soluble collagen contents, flesh chewiness and free flavor amino acids contents (P < 0.05). The supplementation of VC (FM20C) increased the contents of total collagen, heat-insoluble collagen and free flavor amino acids, and decreased flesh lactic acid, malondialdehyde contents and steaming loss (P < 0.05). The combined supplementation of Hyp and VC (FM20HC) showed higher total collagen and heat-insoluble collagen contents than the individual supplementation (FM20H and FM20C) (P < 0.05), and the myofiber density, flesh hardness and shear force in the FM20HC group were also significantly higher than those in the control (P < 0.05). In summary, the combined supplementation of hydroxyproline and VC in practical diet with fishmeal content of 200 g/kg significantly improved the growth performance and flesh quality of L. vannamei cultured in low salinity water.
本试验研究了实用饲料中添加羟脯氨酸(Hyp)和维生素C (VC)对凡纳滨对虾(Litopenaeus vannamei)生长和肉质的影响。在对照饲料中添加200 g/kg鱼粉(FM20),然后在对照饲料中添加14 g/kg包被羟脯氨酸(50%)(FM20H)、2 g/kg l-抗坏血酸-2-磷酸(38%)(FM20C)及其组合(FM20HC),组成4种异蛋白(41.6%)和等脂(6.7%)饲料。初始体重为(1.50±0.05)g的太平洋白对虾在低盐度(盐度为1‰)水中投喂上述4种饲料,为期8周。结果表明:饲粮中添加Hyp和VC (FM20HC)可使肉鸡增重提高13.4%,饲料系数降低0.18 (P <;0.05),而单独添加(FM20H和FM20C组)仅在数值上促进了生长(P >;0.05)。此外,FM20HC组也比FM20组表现出更高的蛋白质和脂质潴留(P <;0.05)。肉质方面,添加Hyp (FM20H)提高了总胶原蛋白和热溶性胶原蛋白含量、肉质的咀嚼性和游离风味氨基酸含量(P <;0.05)。VC (FM20C)的添加提高了总胶原蛋白、热不溶性胶原蛋白和游离风味氨基酸的含量,降低了肉乳酸、丙二醛含量和蒸损(P <;0.05)。与单独添加FM20H和FM20C相比,Hyp和VC (FM20HC)联合添加的总胶原和热不溶性胶原含量更高(P <;0.05), FM20HC组肌纤维密度、肌体硬度和剪切力也显著高于对照组(P <;0.05)。综上所述,在鱼粉含量为200 g/kg的实用饲料中添加羟脯氨酸和VC,可显著提高凡纳滨对虾在低盐度水中的生长性能和肉质。
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引用次数: 0
Different types of dietary carotenoids improve the color and odor quality of Eriocheir sinensis ovaries 不同种类的类胡萝卜素能改善中华鳖卵巢的颜色和气味质量
Q1 Agricultural and Biological Sciences Pub Date : 2025-05-01 DOI: 10.1016/j.aaf.2024.01.001
Long Zhang , Jingjing Wu , Xiaodong Jiang , Xugan Wu , Xichang Wang
Chinese mitten crab, Eriocheir sinensis, is a commercially ecological crab species in China. Ovaries are one of the major edible parts for female E. sinensis, and their color and flavor primarily affect consumer acceptance. This study investigated the effects of different dietary carotenoids on the color and odor quality of adult female E. sinensis ovaries. The crab was fed the experiment diets with supplementation of 100 ×106 of β-carotene, astaxanthin, lutein, canthaxanthin and zeaxanthin, respectively, for 70 days, while the control diet without the supplementation of carotenoids. The results showed that different carotenoids generated an orange-red coloration on crab ovaries, and astaxanthin and canthaxanthin significantly increased the redness (P < 0.05). All carotenoid groups had higher total carotenoids and fatty acid contents than the control group. Moreover, β-carotene significantly promoted ovarian carotenoid deposition, and lutein predominantly affected fatty acid accumulation (P < 0.05). β-carotene and lutein groups accumulated more aroma components (alcohols, ketones, and aldehydes) by regulating the metabolism of carotenoid and fatty acids in ovaries. These results show that canthaxanthin is a potential astaxanthin substitute for promoting coloration, while β-carotene and lutein can improve the odor quality of the ovaries of adult female E. sinensis.
中华绒螯蟹(Eriocheir sinensis)是中国一种商业生态蟹种。子房是雌性中华梭子鱼的主要食用部位之一,其颜色和风味是影响消费者接受度的主要因素。本试验研究了饲料中添加不同的类胡萝卜素对成虫雌性卵巢颜色和气味品质的影响。试验饲粮中分别添加100 ×10−6的β-胡萝卜素、虾青素、叶黄素、角黄素和玉米黄质,饲喂70 d,对照组不添加类胡萝卜素。结果表明,不同的类胡萝卜素使蟹子房呈现橙红色,虾青素和角黄素显著增加了蟹子房的红色(P <;0.05)。所有类胡萝卜素组的总类胡萝卜素和脂肪酸含量均高于对照组。此外,β-胡萝卜素显著促进卵巢类胡萝卜素沉积,叶黄素主要影响脂肪酸积累(P <;0.05)。β-胡萝卜素和叶黄素组通过调节卵巢中类胡萝卜素和脂肪酸的代谢,积累了更多的香气成分(醇、酮和醛)。综上所述,角黄素是一种潜在的虾青素替代品,具有促进显色的作用,而β-胡萝卜素和叶黄素可以改善成虫雌性卵巢的气味质量。
{"title":"Different types of dietary carotenoids improve the color and odor quality of Eriocheir sinensis ovaries","authors":"Long Zhang ,&nbsp;Jingjing Wu ,&nbsp;Xiaodong Jiang ,&nbsp;Xugan Wu ,&nbsp;Xichang Wang","doi":"10.1016/j.aaf.2024.01.001","DOIUrl":"10.1016/j.aaf.2024.01.001","url":null,"abstract":"<div><div>Chinese mitten crab, <em>Eriocheir sinensis</em>, is a commercially ecological crab species in China. Ovaries are one of the major edible parts for female <em>E. sinensis</em>, and their color and flavor primarily affect consumer acceptance. This study investigated the effects of different dietary carotenoids on the color and odor quality of adult female <em>E. sinensis</em> ovaries. The crab was fed the experiment diets with supplementation of 100 ×10<sup>−</sup><sup>6</sup> of β-carotene, astaxanthin, lutein, canthaxanthin and zeaxanthin, respectively, for 70 days, while the control diet without the supplementation of carotenoids. The results showed that different carotenoids generated an orange-red coloration on crab ovaries, and astaxanthin and canthaxanthin significantly increased the redness (<em>P</em> &lt; 0.05). All carotenoid groups had higher total carotenoids and fatty acid contents than the control group. Moreover, β-carotene significantly promoted ovarian carotenoid deposition, and lutein predominantly affected fatty acid accumulation (<em>P</em> &lt; 0.05). β-carotene and lutein groups accumulated more aroma components (alcohols, ketones, and aldehydes) by regulating the metabolism of carotenoid and fatty acids in ovaries. These results show that canthaxanthin is a potential astaxanthin substitute for promoting coloration, while β-carotene and lutein can improve the odor quality of the ovaries of adult female <em>E. sinensis</em>.</div></div>","PeriodicalId":36894,"journal":{"name":"Aquaculture and Fisheries","volume":"10 3","pages":"Pages 475-484"},"PeriodicalIF":0.0,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139888465","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Toxicity and therapeutical impacts of Bee venom (Apis mellifera L.) on Nile tilapia juvenile (Oreochromis niloticus) 蜂毒(Apis mellifera L.)对尼罗罗非鱼幼鱼(Oreochromis niloticus)的毒性和治疗影响
Q1 Agricultural and Biological Sciences Pub Date : 2025-05-01 DOI: 10.1016/j.aaf.2024.02.002
Mohammed F. El Basuini , Haitham M. Ramadan , Abdelaziz M. El-Hais , Mohamed A.A. Zaki , Nourhan M. Kamel , Islam I. Teiba , Emad H. El-Bilawy , Mohamed Reda Badr , Mohamed Fathy Abdel-Aziz , Akram Ismael Shehata
Bee venom (BV) has been widely studied for its therapeutic properties in various medical applications. However, its effects on aquatic organisms, particularly Nile tilapia (Oreochromis niloticus), remain poorly understood, particularly concerning toxicity and therapeutic impacts. This study investigated the effects of bee venom (BV) injection on Nile tilapia (O. niloticus) by examining growth, feed efficiency, biometric parameters, whole-body chemical analysis, and the activity of antioxidant enzymes in the liver. Nile tilapia juveniles were injected with BV at doses of 0, 3, 6, 9, and 12 mg/kg of fish weight. Observations were made on the 14th day post-injection (DPI). No significant differences were found in growth, feed utilization, survival rate, biometric indices, and whole-body composition among the BV dosage groups. Similarly, the activity of intestinal enzymes (amylase, protease, and lipase) showed no significant changes. However, the study revealed a time-dependent response of antioxidant enzymes (SOD, CAT, and GPx) in the liver, with activity peaks on the 4th and 6th DPI. On the 14th DPI, fish injected with BV at doses of 3, 6, and 9 mg/kg exhibited higher antioxidant enzyme activities compared to the control and the highest dosage group (12 mg/kg). These results imply the presence of potential adaptive antioxidant activity and a favorable influence on the health and immune responses of Nile tilapia following BV injection. Notably, the effects appear to vary with the dose, influencing both antioxidant enzyme activities and immunity. Further research is warranted to explore the long-term effects of BV exposure and its potential therapeutic applications in aquaculture.
蜂毒(BV)因其治疗特性在各种医学应用中得到了广泛的研究。然而,其对水生生物,特别是尼罗罗非鱼(Oreochromis niloticus)的影响仍然知之甚少,特别是在毒性和治疗影响方面。本研究通过考察尼罗罗非鱼(O. niloticus)的生长、饲料效率、生物特征参数、全身化学分析和肝脏抗氧化酶活性,研究了蜂毒(BV)注射对尼罗罗非鱼(O. niloticus)的影响。将尼罗罗非鱼幼鱼按每公斤鱼重0、3、6、9和12毫克的剂量注射BV。注射后第14天进行观察。BV给药组间生长、饲料利用率、存活率、生物特征指标和全身组成均无显著差异。肠道酶(淀粉酶、蛋白酶和脂肪酶)的活性也无明显变化。然而,该研究揭示了肝脏中抗氧化酶(SOD, CAT和GPx)的时间依赖性反应,其活性在第4和第6 DPI时达到峰值。在第14 DPI时,与对照组和最高剂量组(12 mg/kg)相比,注射3、6和9 mg/kg BV的鱼表现出更高的抗氧化酶活性。这些结果表明BV注射后存在潜在的适应性抗氧化活性,对尼罗罗非鱼的健康和免疫应答有良好的影响。值得注意的是,效果似乎随剂量而变化,影响抗氧化酶活性和免疫力。有必要进一步研究细菌性阴道炎暴露的长期影响及其在水产养殖中的潜在治疗应用。
{"title":"Toxicity and therapeutical impacts of Bee venom (Apis mellifera L.) on Nile tilapia juvenile (Oreochromis niloticus)","authors":"Mohammed F. El Basuini ,&nbsp;Haitham M. Ramadan ,&nbsp;Abdelaziz M. El-Hais ,&nbsp;Mohamed A.A. Zaki ,&nbsp;Nourhan M. Kamel ,&nbsp;Islam I. Teiba ,&nbsp;Emad H. El-Bilawy ,&nbsp;Mohamed Reda Badr ,&nbsp;Mohamed Fathy Abdel-Aziz ,&nbsp;Akram Ismael Shehata","doi":"10.1016/j.aaf.2024.02.002","DOIUrl":"10.1016/j.aaf.2024.02.002","url":null,"abstract":"<div><div>Bee venom (BV) has been widely studied for its therapeutic properties in various medical applications. However, its effects on aquatic organisms, particularly Nile tilapia (<em>Oreochromis niloticus</em>), remain poorly understood, particularly concerning toxicity and therapeutic impacts. This study investigated the effects of bee venom (BV) injection on Nile tilapia (<em>O. niloticus</em>) by examining growth, feed efficiency, biometric parameters, whole-body chemical analysis, and the activity of antioxidant enzymes in the liver. Nile tilapia juveniles were injected with BV at doses of 0, 3, 6, 9, and 12 mg/kg of fish weight. Observations were made on the 14th day post-injection (DPI). No significant differences were found in growth, feed utilization, survival rate, biometric indices, and whole-body composition among the BV dosage groups. Similarly, the activity of intestinal enzymes (amylase, protease, and lipase) showed no significant changes. However, the study revealed a time-dependent response of antioxidant enzymes (SOD, CAT, and GPx) in the liver, with activity peaks on the 4th and 6th DPI. On the 14th DPI, fish injected with BV at doses of 3, 6, and 9 mg/kg exhibited higher antioxidant enzyme activities compared to the control and the highest dosage group (12 mg/kg). These results imply the presence of potential adaptive antioxidant activity and a favorable influence on the health and immune responses of Nile tilapia following BV injection. Notably, the effects appear to vary with the dose, influencing both antioxidant enzyme activities and immunity. Further research is warranted to explore the long-term effects of BV exposure and its potential therapeutic applications in aquaculture.</div></div>","PeriodicalId":36894,"journal":{"name":"Aquaculture and Fisheries","volume":"10 3","pages":"Pages 429-441"},"PeriodicalIF":0.0,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140090714","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Transposon insertion in pmel17 rewired skin and muscle transcriptomes in Mozambique tilapia 莫桑比克罗非鱼皮肤和肌肉转录组中pmel17转座子的插入
Q1 Agricultural and Biological Sciences Pub Date : 2025-05-01 DOI: 10.1016/j.aaf.2023.12.002
Fei Sun , Le Wang , Gen Hua Yue
The pmel17 gene plays a crucial role in melanin pigmentation. Our previous studies showed that in Mozambique tilapia, a transposon inserted into the 3′ untranslated region (3′ UTR) of pmel17 resulted in the silencing of pmel17 and led to the loss of melanin pigments (golden mutant phenotype). Additionally, the transposon insertion caused reduced growth performance and increased locomotion. In this study, to investigate the mechanisms underlying these phenotypic changes, we sequenced transcriptomes of the skin and muscle samples collected from wildtype and mutant tilapias. A total of 51 and 141 differentially expressed genes (DEGs) were identified in the skin and muscle transcriptomes, respectively. DEGs in the skin were primarily down-regulated in golden genotypes and associated with neural crest development and melanin pigmentation pathways. Besides these DEGs involved in the classic melanin pigmentation pathway of vertebrates, 14 DEGs were also observed to be related to melanogenesis. In muscle transcriptomes, there was an enrichment of GO terms associated with growth factors and cellular lipid catabolic processes. Specifically, DEGs related to growth factor binding exhibited a down-regulation, while those related to lipid metabolism showed an up-regulation in mutant genotypes. These findings agree with observed phenotypic changes. Furthermore, several DEGs associated with muscle function and mobility were up-regulated. Our study sheds light on how a single mutation in a gene can modulate multiple phenotypes by rewiring gene regulation networks. The research also provides valuable insights into the complex genetic mechanisms underlying the regulation of diverse phenotypic traits by a single gene.
pmel17基因在黑色素沉着中起着关键作用。我们之前的研究表明,在莫桑比克罗非鱼中,一个转座子插入pmel17的3 ‘非翻译区(3 ’ UTR),导致pmel17沉默,导致黑色素色素的损失(黄金突变表型)。此外,转座子插入导致生长性能下降和运动增加。在这项研究中,为了研究这些表型变化的机制,我们对野生型和突变型罗非鱼的皮肤和肌肉样本的转录组进行了测序。在皮肤和肌肉转录组中分别鉴定出51个和141个差异表达基因(deg)。在金色基因型中,皮肤中的DEGs主要下调,并与神经嵴发育和黑色素色素沉着途径相关。除了这些基因参与经典的脊椎动物黑色素沉着途径外,还观察到14个基因与黑色素形成有关。在肌肉转录组中,与生长因子和细胞脂质分解代谢过程相关的氧化石墨烯术语富集。具体来说,在突变基因型中,与生长因子结合相关的deg表现为下调,而与脂质代谢相关的deg表现为上调。这些发现与观察到的表型变化一致。此外,一些与肌肉功能和活动相关的deg被上调。我们的研究揭示了基因中的单个突变如何通过重新连接基因调控网络来调节多种表型。该研究还为单个基因调控多种表型性状的复杂遗传机制提供了有价值的见解。
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引用次数: 0
Domestication of manggabai fish (Glossogobius giuris) through adaptation to different types of feed and aquaculture containers 通过适应不同类型的饲料和水产养殖容器驯化芒加贝鱼(Glossogobius giuris)。
Q1 Agricultural and Biological Sciences Pub Date : 2025-05-01 DOI: 10.1016/j.aaf.2023.12.005
Yuniarti Koniyo, Juliana
This study aims to determine the growth and survival of Manggabai fish (Glossogobius giuris) cultivated in different types of feed and maintenance containers. The method used in the study was an experimental method using a 2 × 3 Factorial Complete Randomized Design (CRD), namely two levels of rearing container factors and three levels of feed type factors. The container consists of two types of containers, namely aquarium and concrete tub. The feed given consists of three types, namely Daphnia sp., Tubifex sp. And pellets. The research variables consisted of growth and survival of test animals. The test animals used were Manggabai fish with a total length of (3 ± 0.05) cm and body weight of 4 ± 0.05 g. Data analysis in this study used a two-factor Completely Randomized Design with a probability level of P < 0.05 and used descriptive analysis of length, weight, and survival growth data during the maintenance of test animals. The results showed that the use of different feed and maintenance containers had a significant effect on the growth and survival of manggabai fish. Total absolute weight growth of manggabai fish reared in concrete containers and fed with Daphnia sp. 22.19 g, pellets 22.99 g, and those fed with Tubifex sp. Feed amounted to 39.63 g. The highest growth and survival were obtained in the treatment using a concrete tank and Tubifex sp. Feed. The highest growth and survival were obtained in the treatment using a concrete tank and Tubifex sp. Feed. The use of aquarium maintenance containers and the provision of pellets is not recommended for domestication of Manggabai fish, because it provides the lowest growth and survival.
本研究旨在确定在不同类型的饲料和维持容器中养殖的曼加白鱼(Glossogobius giuris)的生长和存活情况。本研究采用2 × 3全因子完全随机设计(CRD)的试验方法,即2个水平的饲养容器因素和3个水平的饲料类型因素。该容器由两种容器组成,即水族箱和混凝土桶。所给的饲料有三种,即水蚤、管蚤和颗粒。研究变量包括试验动物的生长和存活。试验动物为长(3±0.05)cm、体重4±0.05 g的芒嘎白鱼。本研究的数据分析采用双因素完全随机设计,概率水平为P <;0.05,并对试验动物维持期间的长度、体重和存活生长数据进行描述性分析。结果表明,使用不同的饲料和维持容器对芒加白鱼的生长和存活有显著影响。在混凝土容器中饲养,水蚤饲料22.19 g,颗粒饲料22.99 g, Tubifex饲料39.63 g。用混凝土池和Tubifex sp.饲料处理的生长和成活率最高。用混凝土池和Tubifex sp.饲料处理的生长和成活率最高。不建议使用水族馆养护容器和提供颗粒来驯化mangabbai鱼,因为它提供的生长和存活率最低。
{"title":"Domestication of manggabai fish (Glossogobius giuris) through adaptation to different types of feed and aquaculture containers","authors":"Yuniarti Koniyo,&nbsp;Juliana","doi":"10.1016/j.aaf.2023.12.005","DOIUrl":"10.1016/j.aaf.2023.12.005","url":null,"abstract":"<div><div>This study aims to determine the growth and survival of Manggabai fish (Glossogobius giuris) cultivated in different types of feed and maintenance containers. The method used in the study was an experimental method using a 2 × 3 Factorial Complete Randomized Design (CRD), namely two levels of rearing container factors and three levels of feed type factors. The container consists of two types of containers, namely aquarium and concrete tub. The feed given consists of three types, namely <em>Daphnia</em> sp., <em>Tubifex</em> sp. And pellets. The research variables consisted of growth and survival of test animals. The test animals used were Manggabai fish with a total length of (3 ± 0.05) cm and body weight of 4 ± 0.05 g. Data analysis in this study used a two-factor Completely Randomized Design with a probability level of <em>P</em> &lt; 0.05 and used descriptive analysis of length, weight, and survival growth data during the maintenance of test animals. The results showed that the use of different feed and maintenance containers had a significant effect on the growth and survival of manggabai fish. Total absolute weight growth of manggabai fish reared in concrete containers and fed with <em>Daphnia</em> sp. 22.19 g, pellets 22.99 g, and those fed with <em>Tubifex</em> sp. Feed amounted to 39.63 g. The highest growth and survival were obtained in the treatment using a concrete tank and <em>Tubifex</em> sp. Feed. The highest growth and survival were obtained in the treatment using a concrete tank and <em>Tubifex</em> sp. Feed. The use of aquarium maintenance containers and the provision of pellets is not recommended for domestication of Manggabai fish, because it provides the lowest growth and survival.</div></div>","PeriodicalId":36894,"journal":{"name":"Aquaculture and Fisheries","volume":"10 3","pages":"Pages 514-521"},"PeriodicalIF":0.0,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139455791","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Understanding size selectivity of trawls using structural models: Methodology and a case study on fish sorting grids 利用结构模型了解拖网的尺寸选择性:鱼类分类网格的方法论和案例研究
Q1 Agricultural and Biological Sciences Pub Date : 2025-05-01 DOI: 10.1016/j.aaf.2024.03.003
Nadine Jacques , Bent Herrmann , Jesse Brinkhof , Manu Sistiaga
Fish behaviour affects the performance of selection devices in fishing gears. Traditionally, fish behaviour in relation to selection devices is assessed by direct observation. However, this approach has limitations, and the observations are not explicitly incorporated in the selectivity models. Further, underwater observations and quantification of fish behaviour can be challenging. In this study we outline and use an indirect method to explicitly incorporate and quantify fish behaviour in trawl selectivity analysis. We use a set of structural models, which are based on modelling the actual processes believed to determine the size selection of the device, to discern which behaviours are most likely to explain the selectivity process. By bootstrapping we assess how confident we can be in the choice of a specific structural model and on discerning the associated behavioural aspects. We collected size selectivity data in the Barents Sea demersal trawl fishery targeting gadoids, where the use of a sorting grid is compulsory. Using our modelling approach, we obtained deeper understanding of which behavioural processes most likely affect size selectivity in the sorting grids tested. Our approach can be applied to other fishing gears to understand and quantify fish behaviour in relation to size selectivity.
鱼类行为影响渔具中选择装置的性能。传统上,与选择装置有关的鱼类行为是通过直接观察来评估的。然而,这种方法有局限性,并且观测结果没有明确地纳入选择性模型。此外,水下观察和鱼类行为的量化可能具有挑战性。在这项研究中,我们概述并使用一种间接的方法来明确地纳入和量化拖网选择性分析中的鱼类行为。我们使用了一套结构模型,这些模型基于对确定设备尺寸选择的实际过程的建模,以辨别哪些行为最有可能解释选择过程。通过自我引导,我们可以评估我们在选择特定结构模型和辨别相关行为方面的信心。我们收集了巴伦支海海底拖网渔业的尺寸选择性数据,目标是gadoids,其中使用分类网格是强制性的。使用我们的建模方法,我们更深入地了解了哪些行为过程最有可能影响所测试的分类网格的大小选择。我们的方法可以应用于其他渔具,以了解和量化与尺寸选择性有关的鱼类行为。
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引用次数: 0
The potential contribution of Indonesian fishing vessels in reducing Green House gas emission 印尼渔船在减少温室气体排放方面的潜在贡献
Q1 Agricultural and Biological Sciences Pub Date : 2025-05-01 DOI: 10.1016/j.aaf.2024.08.002
Suryanto Suryanto, Duto Nugroho, Umi Muawanah, Setiya Triharyuni, Dian Oktaviani, Sandi Wibowo, Novi Susetyo Adi, Puput Dani Prasetyo Adi
In common practices, fishing vessels' Greenhouse Gas (GHG) emission is a part of the energy sector. Annual series data show Indonesian fishing vessels are growing by more than 18,000 units/year, dominated by fishing vessels with a tonnage of less than 30 GT. A ′bottom-up' approach was applied to develop low-carbon fishing activity practices to contribute to GHG emission reduction. This study focuses on a GHG inventory of fishing vessels managed by local and central Governments, using data from fishing permits and vessel monitoring systems from 2010 to 2018. The result is expected to act as the baseline for estimating emissions of fishing vessels up to 2030. The model predicted it could be reduced in 2030 by up to 6.43 E+07 tons CO2eq (68%) for fishing vessels with a size of less than 30 GT and up to 5.80E+06 tons CO2eq (84.5%) for a larger size of fishing vessels, respectively. To achieve further mitigation targets, mixing diesel oil using 30% biodiesel should be implemented progressively by 10% annually from 2021. Concerning fisher welfare after 2015, it shows a positive trend along with energy efficiency, and it can be concluded that strategies to improve the energy efficiency of active fishing vessels should be considered urgent.
通常情况下,渔船的温室气体(GHG)排放是能源部门的一部分。年度系列数据显示,印尼渔船每年增长超过1.8万艘,其中以吨位低于30亿吨的渔船为主。采用“自下而上”的方法开发低碳捕捞活动,为减少温室气体排放做出贡献。本研究的重点是地方和中央政府管理的渔船温室气体清单,使用了2010年至2018年捕捞许可证和船舶监测系统的数据。预计该结果将作为估计到2030年渔船排放量的基准。该模型预测,到2030年,对于规模小于30 GT的渔船,可减少高达6.43 E+07吨二氧化碳当量(68%),对于规模较大的渔船,可减少高达5.80E+06吨二氧化碳当量(84.5%)。为了实现进一步的减排目标,从2021年开始,应以每年10%的速度逐步实施使用30%生物柴油的混合柴油。2015年后的渔民福利与能源效率呈正相关趋势,提高现役渔船能源效率的策略迫在眉睫。
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引用次数: 0
Evaluation of growth performances of crossbreds and backcrossbreds with purebreds Nishikigoi and Hungarian carp (Cyprinus carpio) 杂交种和回交种与纯种西乡鲤和匈牙利鲤鱼(Cyprinus carpio)生长性能的评估
Q1 Agricultural and Biological Sciences Pub Date : 2025-05-01 DOI: 10.1016/j.aaf.2023.11.003
Vander Bruno Santos, Lucas Rosan Furquim
This study aimed to evaluate growth curves among different common carp genotypes, according to fingerling performance and morphometry. Fingerlings selected according to Nishikigoi pattern, either (9.66 ± 1.47) cm in standard length, from crossbred and pure carp, respectively, were cultivated in 8 cages (4 replicates for each genotype), measuring 9 m³ (3.0 m × 2.0 m × 1.5 m) each, in a 1500 m2 (12 m × 125 m) pond at a depth of 1.60 m with 10 fingerlings/m³. Fish were fed four times a day at the rate of 5%–7% of biomass with the same specific diet for each growth stage. Forty carp of each genotype (10 fish/cage) were weighed at days 0, 60, 150, 270 and 330 of cultivation. They were weighed and measured for head length, standard length, body height and body width. The Gompertz model was fit for weight × age data for comparison of absolute and relative growth rate, weight and age at the inflection points. The crossbred genotype showed better survival, weight gain and feed conversion compared to pure carp (P < 0.05). The final weight estimate at 330 days was 38.45% higher in the crossbred genotype (289.16 g) when compared to the pure carp (208.85 g). The pure genotype presented lower weight (431.60 g), age (559.71 days) and absolute growth rate (1.03 g/day) at the inflection points when compared to the crossbred genotype (587.69 g; 559.71 days and 1.46 g/day, respectively). Body morphometric relationships changed with fish growth, and this occurred differently, depending on carp genotype evaluated. From these results, it can be concluded that crossing Nishikigoi with Hungarian carp can be a strategy to improve desirable characteristics in koi.
本研究的目的是根据鱼种性能和形态测定来评价不同基因型鲤鱼的生长曲线。按Nishikigoi模式选择标准长度为(9.66±1.47)cm的杂交鲤和纯鲤鱼种,分别饲养于8个网箱中(每个基因型4个重复),网箱尺寸为9 m³(3.0 m × 2.0 m × 1.5 m),池深为1.60 m,池深为1500 m2 (12 m × 125 m),每m³10条鱼种。每个生长阶段采用相同的特定饲料,每天投喂4次,投喂量为生物量的5% ~ 7%。分别于养殖第0、60、150、270和330天称重,每种基因型各40条,每笼10条。他们称重并测量了头长、标准长度、身体高度和身体宽度。采用Gompertz模型对体重×年龄数据进行拟合,比较绝对增长率和相对增长率,以及拐点处的体重和年龄。与纯种鲤鱼相比,杂交基因型的成活率、增重和饲料系数更高(P <;0.05)。杂交基因型在330天的最终体重估计(289.16 g)比纯鲤鱼(208.85 g)高38.45%。纯基因型在拐点处的体重(431.60 g)、日龄(559.71 d)和绝对生长率(1.03 g/d)均低于杂交基因型(587.69 g;559.71 d和1.46 g/d)。鱼体形态计量学的关系随着鱼的生长而变化,而且这种变化的方式因所评估的基因型不同而不同。从这些结果可以得出结论,西锦井与匈牙利鲤鱼杂交可以改善锦鲤的理想特性。
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引用次数: 0
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Aquaculture and Fisheries
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