Veterinary and Animal Science最新文献_第3页

Prevalence and economic impact of subclinical ketosis on smallholder farms of Holstein-Friesian-Zebu crossbred cows 小农场荷斯泰因-弗里西亚-泽布杂交奶牛亚临床酮症的流行及经济影响

IF 1.9 Q2 AGRICULTURE, DAIRY & ANIMAL SCIENCE

Veterinary and Animal Science

Pub Date : 2025-12-15 DOI: 10.1016/j.vas.2025.100556

Kassahun Ayana Negasee , Samuel Derso Tezera , Fentahun Mengie Bayilie , Yitayew Demessie , Abebe Belete Bitew , Sefinew Alemu Mekonnen

Ketosis is the excessive accumulation of ketone bodies in the bloodstream in response to a negative energy balance. In Ethiopia, there is limited data on subclinical ketosis. The study aimed to estimate the prevalence and identify factors associated with subclinical ketosis (SCK), as well as measure the economic losses among lactating dairy cows. Cross-sectional and longitudinal study designs were employed. Samples used to assess SCK were blood and milk. A total of 198 cows from 78 dairy farms were included as samples. The level of ketone bodies was estimated from the fat-to-protein ratio (FPR). Data were collected by face-to-face interviews. The milk yield of 32 cows was measured over eight days to estimate the reduction in milk production. Logistic regression models were used to identify factors associated with the prevalence of SCK. The prevalence of SCK was 57 %. Cows with concentrate feeding, with mastitis, and with >50 % Holstein Friesian blood level had higher odds of SCK. Poor body condition was associated with reduced odds of SCK , whereas increased parity increased the odds of SCK. SCK resulted in a daily milk loss of 0.8 liters per cow, which resulted in average annual losses of 5182 Ethiopian Birr (ETB) per farm (1 ETB = 0.023 US Dollars) and 2445 Birr per cow. SCK is a problem in crossbred dairy cows that contributes to significant economic losses, with a negative impact on the income of smallholder dairy farmers. It emphasizes the need for improved awareness, dairy extension services, and better health management practices to mitigate the impact of SCK on smallholder dairy farms.

酮症是由于负能量平衡导致的血液中酮体的过度积累。在埃塞俄比亚，关于亚临床酮症的数据有限。该研究旨在估计亚临床酮症（SCK）的患病率，确定与SCK相关的因素，并测量泌乳奶牛的经济损失。采用横断面和纵向研究设计。用于评估SCK的样本是血液和牛奶。共选取78个奶牛场的198头奶牛作为样本。酮体的水平是通过脂肪与蛋白质的比值（FPR）来估计的。数据采用面对面访谈的方式收集。在8天内测量了32头奶牛的产奶量，以估计产奶量的减少。使用逻辑回归模型来确定与SCK患病率相关的因素。SCK患病率为57%。精料饲喂、乳腺炎和50%荷斯坦弗里马血水平的奶牛SCK发生率较高。身体状况差与SCK的几率降低有关，而胎次增加则会增加SCK的几率。SCK导致每头奶牛每天损失0.8升牛奶，这导致每个农场平均每年损失5182埃塞俄比亚比尔（ETB）（1 ETB = 0.023美元）和每头奶牛2445比尔。SCK是杂交奶牛的一个问题，造成了重大的经济损失，对小农户的收入产生了负面影响。报告强调需要提高认识，提供奶业推广服务，改善健康管理做法，以减轻SCK对小农奶牛场的影响。

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引用次数: 0

Effect of the high dietary inclusion of wheat dried distillers’ grains with solubles with and without exogenous enzyme supplementation in broiler diet on performance, litter quality and certain animal welfare issues 添加和不添加外源酶时，高添加小麦干酒糟可溶物对肉鸡生产性能、产仔质量和某些动物福利的影响

IF 1.9 Q2 AGRICULTURE, DAIRY & ANIMAL SCIENCE

Veterinary and Animal Science

Pub Date : 2025-12-15 DOI: 10.1016/j.vas.2025.100557

Márk Tóth , Yazavinder Singh , Krisztián Balogh , Erika Zándoki , Szabina Kulcsár , Benjámin Kövesi , Zsolt Ancsin , Tibor Pap , Miklós Mézes , Mária Kovács-Weber , Márta Erdélyi

The study assessed the effects of high dietary inclusion of wheat dried distillers’ grains with solubles (WDDGS), with or without a non-starch polysaccharide (NSP) degrading enzyme (Enzyme) supplement, on broiler performance, carcass traits, meat quality, litter condition, and footpad dermatitis (FPD). Three diets: Control, 30 % WDDGS, and 30 % WDDGS+Enzyme, were fed to 312 Cobb 500 broilers over 42 days. From day 7 onward, WDDGS and Enzyme groups exhibited significantly higher body weight (BW) and weight gain (WG) compared to Control (P < 0.001), with no differences in feed conversion ratio (FCR) or mortality. At day 42, slaughter, carcass, breast, and leg weights increased in WDDGS groups vs Control (P < 0.001), while relative yields remained unchanged. Breast meat pH, water-holding capacity (WHC), shear force, and proximate composition did not differ among treatments; however, meat yellowness (b*) decreased in WDDGS groups (P < 0.001). Litter moisture and FPD scores rose in the WDDGS group (P < 0.001), but enzyme supplementation partially mitigated these welfare concerns, reducing both parameters though not to Control levels. These findings indicate that diets containing 30 % WDDGS can enhance growth performance and carcass weights without compromising meat quality, but may impair litter conditions and increase FPD incidence. Supplementation with NSP-degrading enzymes did not affect production or meat characteristics but improved litter moisture and reduced FPD occurrence. The nutritional feasibility of high-level WDDGS inclusion is confirmed; future research should address gut health, nutrient digestibility, and commercial-scale applications.

本试验旨在评价饲粮中添加或不添加非淀粉多糖（NSP）降解酶（酶）对肉仔鸡生产性能、胴体性状、肉品质、窝产状况和脚垫皮炎（FPD）的影响。选取312只科布500肉鸡，饲喂对照、30% WDDGS和30% WDDGS+酶3种饲粮，试验期42 d。从第7天开始，WDDGS组和酶组的体重（BW）和增重（WG）显著高于对照组（P < 0.001），饲料系数和死亡率无显著差异。第42天，WDDGS组的屠宰重量、胴体重量、胸重和腿重均高于对照组（P < 0.001），而相对产量保持不变。不同处理胸脯肉的pH值、持水量、剪切力和近似成分没有差异；WDDGS组肉黄度（b*）降低（P < 0.001）。WDDGS组凋落物水分和FPD评分上升（P < 0.001），但酶补充部分缓解了这些福利问题，降低了这两个参数，但没有达到控制水平。上述结果表明，饲粮中添加30% WDDGS可以在不影响肉品质的情况下提高生长性能和胴体重，但可能会损害产仔条件并增加FPD发生率。补充nsp降解酶不会影响产量或肉质特性，但会改善凋落物水分，减少FPD的发生。证实了高水平WDDGS纳入的营养可行性；未来的研究应着眼于肠道健康、营养物质消化率和商业规模应用。

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引用次数: 0

Genetic testing drives a decline in the occurrence of the canine PRA-related RPGRIP1 variant without an increase in inbreeding 基因检测导致犬pra相关RPGRIP1变异的发生率下降，而近亲繁殖却没有增加

IF 1.9 Q2 AGRICULTURE, DAIRY & ANIMAL SCIENCE

Veterinary and Animal Science

Pub Date : 2025-12-11 DOI: 10.1016/j.vas.2025.100555

Hisashi Ukawa , Ryo Horie , Kai Ataka , Yuki Matsumoto

A variant of the RPGRIP1 gene is a partial causative factor associated with cone-rod dysplasia 1 and progressive retinal atrophy (PRA) in dogs, particularly in purebred miniature dachshunds (DHMs). Although direct-to-consumer genetic testing targeting the RPGRIP1 variant has been actively conducted in Japan, its effect on the DHM population is unknown. Therefore, to address this gap in research, in the present study, we determined the effect of genetic testing by investigating changes in the allele frequency of the RPGRIP1 variant and genetic structures using long-term genetic testing datasets. By assessing the genotypic data of 30,800 DHMs born between 2014 and 2022, we observed a marked decrease in the number of the RPGRIP1 variant homozygous dogs between dogs born in 2019 (11.1%)—when full-scale genetic testing began for puppies—and those born in 2020 (3.4%) (p < 0.001). A subsequent investigation using a single-nucleotide polymorphism array revealed no substantial changes in inbreeding levels (F_ROH) or genome structures among DHM offspring born between 2019 and 2020. These findings suggest a decrease in the allele frequency for several years and that appropriate breeding practices were in effect. Additional analysis of the insurance claim data indicated that the association between RPGRIP1 variant genotype and PRA claim, and PRA was more prevalent in dogs aged 11 years than in younger dogs. RPGRIP1 genetic testing has led to a reduction in the incidence rate of PRA among DHMs with a decline anticipated in the 2030s.

RPGRIP1基因的变异是犬，特别是纯种迷你腊肠犬（DHMs）的锥杆发育不良1和进行性视网膜萎缩（PRA）相关的部分致病因素。尽管针对RPGRIP1变异的直接面向消费者的基因检测已在日本积极开展，但其对DHM人群的影响尚不清楚。因此，为了解决研究中的这一空白，在本研究中，我们通过使用长期基因检测数据集调查RPGRIP1变异的等位基因频率和遗传结构的变化来确定基因检测的影响。通过评估2014年至2022年间出生的30,800只dhm的基因型数据，我们观察到2019年出生的狗（11.1%）（开始对幼犬进行全面基因检测）和2020年出生的狗（3.4%）之间RPGRIP1变异纯合狗的数量显著减少（p < 0.001）。随后使用单核苷酸多态性阵列进行的调查显示，在2019年至2020年出生的DHM后代中，近亲繁殖水平（FROH）或基因组结构没有实质性变化。这些发现表明，等位基因频率在数年内有所下降，适当的育种措施是有效的。对保险索赔数据的进一步分析表明，RPGRIP1变异基因型与PRA索赔之间存在关联，并且PRA在11岁的狗中比在年轻的狗中更为普遍。RPGRIP1基因检测导致dhm中PRA发病率下降，预计在2030年代下降。

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引用次数: 0

Deciphering autosomal and X-linked genetic effects of early growth traits in Murciano-Granadina goats via a multivariate animal model 通过多变量动物模型解读穆尔西亚诺-格拉纳迪纳山羊早期生长性状的常染色体和x连锁遗传效应

IF 1.9 Q2 AGRICULTURE, DAIRY & ANIMAL SCIENCE

Veterinary and Animal Science

Pub Date : 2025-12-07 DOI: 10.1016/j.vas.2025.100553

Jamshid Ehsaninia , Mohammad Bagher Zandi , Moein Taned , Alireza Bagheripour

This study aimed to quantify autosomal and sex-linked genetic parameters for early growth traits in the Murciano-Granadina goat breed. Data were collected from a commercial dairy herd in Ghaleh-Ganj, southern Iran. A total of 19,582 records for birth weight (BWT) and 9157 records for weaning weight (WWT) were analyzed, covering animals born between 2016 and 2023, descended from 460 bucks and 5382 does. Live weight measurements were used to calculate preweaning growth rate (PWGR), preweaning Kleiber ratio (PWKR), and preweaning growth efficiency (PWGE). Environmental effects were evaluated using aov package in R, and the optimal multivariate model was constructed by combining the best-fitting univariate models for each trait. Genetic parameters were estimated using this optimal multivariate animal model and the average information restricted maximum likelihood algorithm in WOMBAT software. Estimated direct autosomal heritabilities (

h_{a}^{2}

) were 0.05 for BWT, 0.08 for WWT, 0.06 for PWGR and PWGE, and 0.09 for PWKR. Corresponding direct sex-linked heritabilities (

h_{s}^{2}

) were 0.03 for BWT, 0.02 for WWT, 0.01 for PWGR, and PWKR, and 0.04 for PWGE. Excluding sex-linked chromosomal effects from optimal model led to a 3–14 % increase in autosomal additive genetic variance and a 7–20 % increase in the residual variance. Autosomal genetic correlations (r_a) ranged from -0.70 (BWT-PWGE) to 0.83 (PWKR-PWGR), while sex-linked genetic correlations (r_s) ranged from -0.75 (BWT-PWGE) to 0.93 (WWT-PWGE). These findings highlight the relevance of accounting for sex-linked additive inheritance in genetic evaluations, supporting more informed selection decisions and contributing to improved genetic progress in Murciano-Granadina goats.

本研究旨在量化Murciano-Granadina山羊品种早期生长性状的常染色体和性别连锁遗传参数。数据收集自伊朗南部Ghaleh-Ganj的一个商业奶牛群。研究人员共分析了出生体重（BWT）的19582条记录和断奶体重（WWT）的9157条记录，涵盖了2016年至2023年出生的动物，从460头公鹿和5382头公鹿下降。测定活重计算断奶前生长率（PWGR）、断奶前克莱伯比（PWKR）和断奶前生长效率（PWGE）。利用R中的aov包对环境效应进行评价，将各性状的最优拟合单变量模型组合，构建最优多变量模型。利用该最优多元动物模型和平均信息限制最大似然算法在WOMBAT软件中估计遗传参数。估计BWT的直接常染色体遗传率（ha2）为0.05，WWT为0.08，PWGR和PWGE为0.06，PWKR为0.09。相应的直接性连锁遗传力（hs2）分别为：BWT为0.03，WWT为0.02，PWGR和PWKR为0.01，PWGE为0.04。从最优模型中排除性别连锁染色体效应导致常染色体加性遗传变异增加3 - 14%，剩余变异增加7 - 20%。常染色体遗传相关性（ra）范围从-0.70 （BWT-PWGE）到0.83 (PWKR-PWGR)，而性别相关遗传相关性（rs）范围从-0.75 （BWT-PWGE）到0.93 （WWT-PWGE）。这些发现强调了在遗传评估中考虑性别连锁加性遗传的相关性，支持了更明智的选择决策，并有助于改善穆尔西亚诺-格拉纳迪纳山羊的遗传进展。

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引用次数: 0

Prokaryotic expression of the giant panda VEGFA gene and the preparation and characterization of its monoclonal antibodies 大熊猫VEGFA基因的原核表达及其单克隆抗体的制备和鉴定

IF 1.9 Q2 AGRICULTURE, DAIRY & ANIMAL SCIENCE

Veterinary and Animal Science

Pub Date : 2025-12-04 DOI: 10.1016/j.vas.2025.100551

Xiangyu Liu , Feiping Li , Shenfei Wang , Mengshi Zhang , Xianbiao Hu , Yuliang Liu , Rong Hou , Kailai Cai

Vascular endothelial growth factor A (VEGFA) plays a crucial role in angiogenesis and is closely associated with two major challenges in giant panda (Ailuropoda melanoleuca) conservation: reproductive disorders and cancer. To support the development of diagnostic and therapeutic tools for this vulnerable species, this study aimed to generate and characterize monoclonal antibodies (mAbs) specific to giant panda VEGFA. The VEGFA gene fragment (35–517) was cloned and expressed as a recombinant protein in Escherichia coli, which was used as an immunogen. Through hybridoma technology, four stable mAb-secreting cell lines were established, all producing IgG2b subclass antibodies. The obtained mAbs were first screened and characterized by indirect enzyme-linked immunosorbent assay (ELISA) using the recombinant VEGFA protein. Their specific recognition of the native VEGFA protein in various giant panda tissues and cells was subsequently confirmed by Western blot analysis. In conclusion, this study successfully generated mAbs against giant panda VEGFA, providing a valuable reagent for future studies on pregnancy complications and cancer in this species.

血管内皮生长因子A （VEGFA）在血管生成中起着至关重要的作用，并与大熊猫（Ailuropoda melanoleuca）保护的两大挑战：生殖障碍和癌症密切相关。为了支持这一脆弱物种的诊断和治疗工具的开发，本研究旨在生成和表征大熊猫VEGFA特异性单克隆抗体（mab）。克隆了VEGFA基因片段（35-517），并在大肠杆菌中表达为重组蛋白，作为免疫原。通过杂交瘤技术，建立了4株稳定的分泌单克隆抗体的细胞系，均产生IgG2b亚类抗体。首先利用重组VEGFA蛋白对获得的单抗进行筛选和间接酶联免疫吸附试验（ELISA）鉴定。随后通过Western blot分析证实了它们对大熊猫各种组织和细胞中天然VEGFA蛋白的特异性识别。综上所述，本研究成功生成了针对大熊猫VEGFA的单克隆抗体，为该物种的妊娠并发症和癌症的进一步研究提供了有价值的试剂。

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引用次数: 0

Molecular detection of Coxiella Burnetii in ovine abortions: evidence from a three-year surveillance in an endemic region 羊流产中伯纳氏杆菌的分子检测：来自流行地区三年监测的证据

IF 1.9 Q2 AGRICULTURE, DAIRY & ANIMAL SCIENCE

Veterinary and Animal Science

Pub Date : 2025-12-04 DOI: 10.1016/j.vas.2025.100550

Pegah Sagha Nosrati , Khadijeh Hashemi , Narges Khaleghnia , Mehrdad Mohri , Pezhman Mirshokraei

Coxiella burnetii, an obligate intracellular coccobacillus, is a major cause of abortion in livestock and a re-emerging zoonotic pathogen responsible for Q fever in humans. Iran, particularly Khorasan-e-Razavi Province, is considered endemic for Q fever. Rapid and accurate detection of C. burnetii in animal shedders, especially asymptomatic ones, is essential to control its spread among animals and from animals to humans.

This study provides the first multi-year (2020–2022) quantitative assessment of C. burnetii infection load in ovine abortions in this endemic region. The objectives were to: (i) develop and validate a region-specific real-time PCR assay targeting the IS1111a gene through sequencing of the amplified fragment; (ii) quantify pathogen load using a standard curve derived from a sequenced reference fragment; and (iii) evaluate associations between prevalence and factors such as year, season, geographic location, and confirmed co-infections with other abortifacient agents. Liver samples from 116 aborted sheep fetuses were examined by real-time PCR, revealing a C. burnetii prevalence of 53.4%, while in 54.8% of these positive cases, C. burnetii was identified as the sole infectious pathogen contributing to abortion. A significant association (p < 0.05) between sampling year and prevalence was observed, with a rising trend over time. The high prevalence of C. burnetii and its role in abortion cases underscore the need for enhanced surveillance and control programs to mitigate the spread and impact of this pathogen in endemic regions.

伯纳蒂克希菌是一种专性细胞内球芽孢杆菌，是牲畜流产的主要原因，也是再次出现的人畜共患病原体，导致人类Q热。伊朗，特别是呼罗珊-拉扎维省，被认为是Q热的地方病。快速、准确地检测动物蜕皮者，特别是无症状蜕皮者的伯纳蒂菌，对于控制其在动物间和动物向人传播至关重要。本研究首次对该流行地区羊流产中的伯纳蒂胞杆菌感染负荷进行了多年（2020-2022）定量评估。目的是：(i)通过对扩增片段进行测序，开发并验证针对IS1111a基因的区域特异性实时PCR检测；（ii）使用从测序参考片段得到的标准曲线定量病原体负荷；（iii）评估患病率与年份、季节、地理位置以及与其他堕胎药物合并感染的确诊病例等因素之间的关系。对116例流产羊胎儿的肝脏样本进行实时PCR检测，发现伯纳蒂胞杆菌感染率为53.4%，其中54.8%的阳性病例中，伯纳蒂胞杆菌是导致流产的唯一感染性病原体。观察到采样年份与患病率之间存在显著关联（p < 0.05），且随时间呈上升趋势。伯纳蒂杆菌的高流行率及其在流产病例中的作用强调了加强监测和控制规划的必要性，以减轻该病原体在流行地区的传播和影响。

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引用次数: 0

Corrigendum to “Body condition score at calving, subclinical ketosis, postpartum body condition score losses, diseases, and fertility in Holstein cows: modelling confounding associations” “荷斯坦奶牛产犊时身体状况评分、亚临床酮症、产后身体状况评分损失、疾病和生育力：模型混淆关联”的勘误

IF 1.9 Q2 AGRICULTURE, DAIRY & ANIMAL SCIENCE

Veterinary and Animal Science

Pub Date : 2025-12-01 DOI: 10.1016/j.vas.2025.100497

Pedro Melendez , Julian Bartolome , Gerardo Gonzalez , Gustavo Lastra-Duran , Pablo Pinedo

引用次数: 0

Corrigendum to ‘Nutrients utilization and enteric methane emission in zebu cattle fed low quality forages’ [Veterinary and Animal Science, Volume 30, December 2025, 100511] “饲喂低质量饲料的瘤牛的养分利用和肠道甲烷排放”的勘误表[兽医与动物科学，第30卷，2025年12月，100511]

IF 1.9 Q2 AGRICULTURE, DAIRY & ANIMAL SCIENCE

Veterinary and Animal Science

Pub Date : 2025-12-01 DOI: 10.1016/j.vas.2025.100533

Gérard Xavier Gbenou , Luc Hippolyte Dossa , Denis Bastianelli , Ollo Sib , Laurent Bonnal , Cécile Martin , Mohamed Habibou Assouma

引用次数: 0

Effect of glutathione, ascorbic acid, and their combination on the quality and fertility of cryopreserved spermatozoa of Black Bengal buck 谷胱甘肽、抗坏血酸及其组合对黑孟加拉鹿冷冻精子质量和育性的影响

IF 1.9 Q2 AGRICULTURE, DAIRY & ANIMAL SCIENCE

Veterinary and Animal Science

Pub Date : 2025-12-01 DOI: 10.1016/j.vas.2025.100549

Md. Abul Bashar , Mst. Mahomudha Akhtar , Shahanaj Ferdousi Shejuty , Gautam Kumar Deb , Sadek Ahmed , M. A. M. Yahia Khandoker

This study investigated the effect of the addition of reduced glutathione (GSH), l-ascorbic acid (AA), and their combination (GSH+AA) to the semen extender on the semen quality and fertility of post-thaw spermatozoa of Black Bengal bucks. Ejaculates (n = 30) collected from five Black Bengal bucks were evaluated, pooled (37 °C), and divided into four treatment groups: a control group (no antioxidants), a GSH group (control + 1 mM GSH), an AA group (control + 8 mM AA), and a combined group (control + 1 mM GSH and 8 mM AA). The semen samples were then filled into 0.25 mL French straws and cryopreserved following a standard cryopreservation method. Supplementation of GSH (1 mM) and AA (8 mM) significantly (P < 0.05) improved total motility, progressive motility, normal morphology (normal fraction, distal droplet, and bent tail), and kinematic parameters (average path velocity, straight-line velocity, curvilinear velocity, and beat cross frequency) of the spermatozoa. However, the maximum improvement was observed with the combined group. In terms of malondialdehyde (MDA) levels and hydrogen peroxide (H₂O₂) status, the values of MDA and H₂O₂ were reduced significantly (P < 0.05) in the GSH, AA, and GSH+AA groups compared to the control group. The fertilization capacity of the cryopreserved spermatozoa was significantly higher (P < 0.05) in the antioxidant-treated groups compared to the control group. In conclusion, supplementation of GSH and AA in freezing medium has a synergistic and beneficial effect on the quality and fertility of cryopreserved spermatozoa of Black Bengal buck.

本试验研究了在精液添加剂中添加还原型谷胱甘肽（GSH）、l-抗坏血酸（AA）及其组合（GSH+AA）对黑孟加拉雄鹿解冻后精子精液质量和育性的影响。从5只黑孟加拉雄鹿收集的射精（n = 30）进行评估，合并（37°C），并分为四个处理组：对照组（无抗氧化剂），GSH组（对照组+ 1 mM GSH）， AA组（对照组+ 8 mM AA）和联合组（对照组+ 1 mM GSH和8 mM AA）。然后将精液样本装入0.25 mL法式吸管中，按照标准冷冻保存方法进行冷冻保存。补充GSH （1 mM）和AA （8 mM）显著（P < 0.05）改善了精子的总运动能力、进行性运动能力、正常形态（正常分数、远端液滴和弯尾）和运动学参数（平均路径速度、直线速度、曲线速度和跳动交叉频率）。然而，观察到最大的改善是联合组。在丙二醛（MDA）水平和过氧化氢（H2O2）状态方面，与对照组相比，GSH、AA和GSH+AA组MDA和H2O2值显著降低（P < 0.05）。各抗氧化剂处理组冷冻保存精子的受精能力显著高于对照组（P < 0.05）。综上所述，在冷冻培养基中添加谷胱甘肽和AA对黑孟加拉鹿冷冻精子的质量和育性具有协同增效作用。

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引用次数: 0

Salmonella in pigs slaughtered in Ecuador: prevalence, serotypes, genotypes and antibiotic resistance 厄瓜多尔屠宰猪中的沙门氏菌：流行情况、血清型、基因型和抗生素耐药性

IF 1.9 Q2 AGRICULTURE, DAIRY & ANIMAL SCIENCE

Veterinary and Animal Science

Pub Date : 2025-12-01 DOI: 10.1016/j.vas.2025.100548

M. Cevallos-Almeida , C. Gómez , B. Cajas , A. Almachi , V. Rose , M. Denis , A. Kerouanton

Salmonella enterica subsp. enterica (S. enterica) is a leading foodborne pathogen worldwide, and pork products are major sources of infection. Given the lack of data on S. enterica infection in pigs slaughtered in Ecuador, this study aimed to determine its prevalence, as well as the serotypes, genotypes and antimicrobial susceptibility profiles of isolates from cecum contents (CCs) and mesenteric lymph nodes (MLNs) sampled from 365 pigs at the evisceration step at a slaughterhouse in Quito city. Salmonella spp. in the samples was detected by culture (ISO 6570–1 method). Strains were serotyped (ISO 6579–3 method), tested for antimicrobial susceptibility using the disk diffusion method, and genotyped by macrorestriction of chromosomal DNA. Salmonella enterica was detected in 56 of the 365 pigs sampled (15.3 %), in CCs (11.2 %) or MLNs (11.2 %) but never in both. The 56 isolates (one per positive sample) belonged to 12 serotypes, mainly the monophasic Salmonella Typhimurium (mST) (28.5 %) and S. Uganda (26.7 %). Twenty-two XbaI PFGE profiles were identified, with higher genetic diversity for S. Infantis and S. Uganda. A high percentage of isolates showed antimicrobial resistance to tetracycline (71.4 %) and streptomycin (62.5 %). Twelve antimicrobial multi-resistant patterns were observed, particularly associated with S. Typhimurium isolates, S. Infantis and mST. The present study constitutes the first investigation of S. enterica prevalence in a large-scale slaughterhouse in Ecuador. The findings will contribute to a better assessment of the epidemiology of S. enterica in the pork industry and of the associated public health risk.

肠道沙门氏菌亚种肠球菌（S. enterica）是世界范围内主要的食源性病原体，猪肉产品是感染的主要来源。鉴于缺乏厄瓜多尔屠宰猪中肠球菌感染的数据，本研究旨在确定其流行情况，以及从基多市屠宰场的365头猪的盲肠内容物（cc）和肠系膜淋巴结（mln）中采集的分离株的血清型、基因型和抗生素敏感性谱。采用培养法（ISO 6570-1）检测样品中的沙门氏菌。采用ISO 6579-3法对菌株进行血清分型，采用纸片扩散法进行药敏试验，采用染色体DNA宏观酶切法进行基因分型。在365头猪中检出56头（15.3%）、cc（11.2%）和MLNs(11.2%)，但两者均未检出沙门氏菌。56株分离株（每阳性1株）分12种血清型，主要为单相鼠伤寒沙门菌（mST）（28.5%）和乌干达沙门菌（26.7%）。鉴定出22个XbaI PFGE图谱，其中S. Infantis和S. Uganda具有较高的遗传多样性。较高比例的分离株对四环素（71.4%）和链霉素（62.5%）具有耐药性。观察到12种抗菌素多重耐药模式，特别是与鼠伤寒沙门氏菌分离株、婴儿沙门氏菌和mST相关。本研究是对厄瓜多尔大型屠宰场中肠球菌流行率的首次调查。研究结果将有助于更好地评估猪肉工业中肠球菌的流行病学以及相关的公共卫生风险。

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引用次数: 0