Veterinary and Animal Science最新文献_第7页

Phenotypic characterization of indigenous Xhosa goat ecotype in three agro-ecological zones in the eastern cape province, South Africa 南非东开普省三个农业生态区土著科萨山羊生态型的表型特征

IF 1.9 Q2 AGRICULTURE, DAIRY & ANIMAL SCIENCE

Veterinary and Animal Science

Pub Date : 2025-09-22 DOI: 10.1016/j.vas.2025.100512

Sibulele Praise Ntonga , Oluwakamisi Festus Akinmoladun , Ziyanda Mpetile

This study characterized the phenotypic attributes of indigenous Xhosa goats across three agro-ecological zones and developed predictive models for estimating body weight. A total of 450 Xhosa goats were sampled using a stratified random approach based on sex, age, and agro-ecological region. Morphometric traits, including body weight (BW), heart girth (HG), body length (BL), wither height (WH), and body depth (RD), were recorded. Data were analysed using the General Linear Model (GLM) of SPSS (v.20) to assess the effects of zones, sex, and age on body traits, while Pearson’s correlation and stepwise regression identified the best predictors of body weight. Results showed significant (p < 0.05) variations in body measurements across zones, with Savanna goats exhibiting superior traits. Males had significantly (p < 0.05) higher BW and body dimensions than females and castrates. Correlation analysis revealed HG (r=0.80), BL (r=0.84), and RD (r=0.82) as the strongest predictors of BW in males, while BL (r=0.66), HG (r=0.65), WH (r=0.62), and RD (r=0.61) were best for females. Stepwise regression identified HG and BL as the best predictors for males, and HG, BL, and SH for females. The predictive models (R² = 0.74–0.85) provide a practical tool for estimating body weight in field conditions. These findings provide practical tools for field-based weight estimation and highlight the importance of conserving the phenotypic diversity of Xhosa goats for sustainable breeding and genetic improvement programs in South Africa.

本研究分析了三个农业生态区科萨山羊的表型特征，并建立了体重预测模型。采用基于性别、年龄和农业生态区的分层随机抽样方法，共取样450只科萨山羊。记录体质量（BW）、胸围（HG）、体长（BL）、枯高（WH）、体深（RD）等形态计量学性状。使用SPSS （v.20）的一般线性模型（GLM）对数据进行分析，以评估区域、性别和年龄对身体特征的影响，而Pearson相关和逐步回归确定了体重的最佳预测因子。结果显示，不同地区的身体测量值存在显著差异（p < 0.05），草原山羊表现出优越的性状。公鼠的体重和体尺显著高于母鼠和去势鼠（p < 0.05）。相关性分析显示，HG （r=0.80）、BL （r=0.84）和RD （r=0.82）是男性体重的最强预测因子，而BL （r=0.66）、HG （r=0.65）、WH （r=0.62）和RD （r=0.61）是女性体重的最佳预测因子。逐步回归发现HG和BL是男性的最佳预测因子，而HG、BL和SH是女性的最佳预测因子。预测模型（R²= 0.74-0.85）为在野外条件下估计体重提供了实用的工具。这些发现为基于田间的体重估计提供了实用工具，并强调了保护科萨山羊表型多样性对南非可持续育种和遗传改良计划的重要性。

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引用次数: 0

Differential effects of flunixin meglumine and meloxicam on TNF- α production in LPS-stimulated equine neutrophils in vitro 氟尼新-大明与美洛昔康对体外lps刺激马中性粒细胞TNF- α生成的差异影响

IF 1.9 Q2 AGRICULTURE, DAIRY & ANIMAL SCIENCE

Veterinary and Animal Science

Pub Date : 2025-09-21 DOI: 10.1016/j.vas.2025.100513

Shuntaro Urayama , Ryo Muko , Masanori Muranaka , Hiroshi Mita , Minoru Ohta , Hiroshi Matsuda , Akane Tanaka

Systemic inflammatory response syndrome (SIRS) induced by endotoxemia is usually secondary to colitis and is a major cause of high morbidity and mortality in horses. Non-steroidal anti-inflammatory drugs (NSAIDs), such as flunixin meglumine (FM) and meloxicam (MX), are used to improve clinical outcomes in SIRS/endotoxemia. These NSAIDs suppress tumor necrosis factor-alpha (TNF-α) levels; however, the underlying mechanisms remain unclear. The aim of this study was to investigate the inhibitory effects of FM and MX on TNF-α in lipopolysaccharide (LPS)-stimulated peripheral blood mononuclear cells (PBMCs) and neutrophils in vitro. Blood samples were collected from three healthy thoroughbred horses, and PBMCs and neutrophils were isolated using density gradient centrifugation. Cells were cultured with LPS (0.3 ng/mL) and FM or MX (5, 10, or 50 μM). TNF-α gene expression was analyzed using quantitative real-time PCR, and protein levels were measured using ELISA. No significant inhibitory effects of FM or MX on TNF-α gene or protein expression were observed in LPS-stimulated PBMCs. However, FM significantly inhibited the increase in TNF-α protein levels in LPS-stimulated neutrophils in a concentration-dependent manner (p < 0.05). MX showed a similar tendency in LPS-stimulated neutrophils, but the differences were not significant. The regulation of neutrophil-derived TNF-α by FM administration could be a promising therapeutic strategy for equine SIRS/endotoxemia, providing mechanistic insight for optimizing anti-inflammatory therapy.

由内毒素血症引起的系统性炎症反应综合征（SIRS）通常继发于结肠炎，是马高发病率和死亡率的主要原因。非甾体类抗炎药（NSAIDs），如氟尼新-甲胺（FM）和美洛昔康（MX），用于改善SIRS/内毒素血症的临床结果。这些非甾体抗炎药抑制肿瘤坏死因子α (TNF-α)水平；然而，潜在的机制仍不清楚。本研究旨在探讨FM和MX对体外脂多糖刺激的外周血单核细胞（pbmc）和中性粒细胞中TNF-α的抑制作用。采集3匹健康纯种马的血液样本，采用密度梯度离心分离pbmc和中性粒细胞。用LPS （0.3 ng/mL）和FM或MX（5、10或50 μM）培养细胞。采用实时荧光定量PCR法分析TNF-α基因表达，ELISA法检测蛋白水平。在lps刺激的PBMCs中，FM和MX对TNF-α基因或蛋白表达均无明显抑制作用。然而，FM显著抑制lps刺激的中性粒细胞中TNF-α蛋白水平的升高，且呈浓度依赖性（p < 0.05）。MX在lps刺激的中性粒细胞中表现出类似的趋势，但差异不显著。通过FM给药调节中性粒细胞来源的TNF-α可能是一种有希望的治疗马SIRS/内毒素血症的策略，为优化抗炎治疗提供机制见解。

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引用次数: 0

Nutrients utilization and enteric methane emission in zebu cattle fed low quality forages 低品质牧草对瘤牛养分利用及肠道甲烷排放的影响

IF 1.9 Q2 AGRICULTURE, DAIRY & ANIMAL SCIENCE

Veterinary and Animal Science

Pub Date : 2025-09-18 DOI: 10.1016/j.vas.2025.100511

Gérard Xavier Gbenou , Luc Hippolyte Dossa , Denis Bastianelli , Ollo Sib , Laurent Bonnal , Cécile Martin , Mohamed Habibou Assouma

During the dry season in sub-Saharan Africa, ruminants are commonly fed low-quality forages, such as rangeland fodder and harvested grasses. This study evaluated the effects of four such forages - rangeland fodder (RF), Brachiaria ruziziensis (Bruzi), Andropogon gayanus (Agaya), and Panicum maximum (Pmaxi), all harvested at maturity (24 months) on feed intake, diet digestibility, and enteric methane (eCH₄) emissions. Ten Sudanese Fulani zebu steers (27.7 ± 4.6 months of age, 143.7 ± 15.7 kg of initial body weight - BW) were fed each forage at 2.3 % and 3.2 % of body weight (dry matter – DM basis) over three-week periods, two weeks of adaptation and one week of data collection. The forages differed significantly in chemical composition, affecting feed intake, diet digestibility, and eCH₄ emissions. The Pmaxi, with superior nutritional quality, was the most consumed and had the highest apparent digestibility (0.49; P < 0.001). At low-quality stage, all the forages had similar eCH₄ yield potential (28.5 - 31.6 g/kg DM intake; P = 0.486). Methane conversion rate (Ym) ranged from 8.6 % in Pmaxi to 9.6 % of gross energy intake in Agaya; however, the variation was not statistically significant. Feeding ruminants over ad libitum low-quality forages did not affect their feed intake, diet apparent digestibility and eCH₄ emissions. Measuring eCH₄ emissions induced by these forages at different growth stages can help identify their optimal use for promoting both feed and environmental sustainability.

在撒哈拉以南非洲的旱季，反刍动物通常被喂食低质量的饲料，如牧场饲料和收获的草。本研究评估了牧场饲料（RF）、ruziziensis （Bruzi）、Andropogon gayanus （Agaya）和Panicum maximum (Pmaxi) 4种牧草在成熟期（24个月）采收对采食量、日粮消化率和肠道甲烷（eCH₄）排放的影响。选取10头苏丹富拉尼瘤牛（27.7±4.6月龄，143.7±15.7 kg初始体重-体重），分别饲喂体重（干物质-干重）的2.3%和3.2%的饲料，为期3周，分别进行2周的适应期和1周的数据收集期。不同饲料的化学成分差异显著，影响采食量、日粮消化率和乙酰胆碱排放。营养品质优良的Pmaxi是消耗量最大、表观消化率最高的品种（0.49;P < 0.001）。在低品质期，所有牧草的eCH4产量潜力相近（采食量28.5 ~ 31.6 g/kg, P = 0.486）。甲烷转化率（Ym）从Pmaxi的8.6%到Agaya的9.6%不等；然而，差异没有统计学意义。随意饲喂低品质饲料对反刍动物采食量、日粮表观消化率和ec4排放均无影响。测量这些牧草在不同生长阶段引起的eCH4排放可以帮助确定它们的最佳利用方式，以促进饲料和环境的可持续性。

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引用次数: 0

Pilot study on the effects of dapagliflozin on echocardiographic parameters in dogs with symptomatic myxomatous mitral valve disease 达格列净对症状性二尖瓣黏液瘤病犬超声心动图参数影响的初步研究

IF 1.9 Q2 AGRICULTURE, DAIRY & ANIMAL SCIENCE

Veterinary and Animal Science

Pub Date : 2025-09-17 DOI: 10.1016/j.vas.2025.100510

Nakkawee Saengklub , Anusak Kijtawornrat , Robert L. Hamlin

Myxomatous mitral valve disease (MMVD) is the most common valvular heart disease in geriatric small-to medium-sized dogs. Dapagliflozin, a sodium-glucose cotransporter-2 inhibitor (SGLT-2i), has shown promise in managing heart failure in human patients. This pilot study aimed to explore the short-term effects of dapagliflozin, when added to conventional therapy in symptomatic MMVD dogs. In a prospective, randomized, single-blind study, five dogs with stage C MMVD received dapagliflozin (0.31 mg/kg, PO, q24h) alongside pimobendan, furosemide, and ramipril. A control group (n = 7) received conventional therapy without dapagliflozin. Echocardiographic, electrocardiographic, blood pressure, blood glucose, NT-proBNP, and urinary glucose data were collected at baseline (D0) and follow-up (D28, D84, and D140). Compared to the control group, dogs receiving dapagliflozin showed significant reductions in the percent change from baseline for left atrial to aortic root ratio, left ventricular internal diameter in diastole normalized to body weight, end-diastolic volume, end-diastolic volume index, end-systolic volume, and end-systolic volume index (P < 0.05). Additionally, ejection fraction significantly increased in the dapagliflozin group (P < 0.05). Glucosuria was consistently present only in the dapagliflozin group. These findings suggest that dapagliflozin, when added to conventional therapy, may promote reverse remodeling and improve cardiac function in dogs with stage C MMVD. Further studies with larger sample sizes and longer follow-up are warranted to validate the cardioprotective effects of dapagliflozin in veterinary patients.

二尖瓣黏液瘤病（MMVD）是最常见的心脏瓣膜病在老年小到中型狗。达格列净是一种钠-葡萄糖共转运蛋白-2抑制剂（SGLT-2i），在治疗人类心力衰竭方面显示出前景。这项初步研究旨在探讨达格列净在有症状的MMVD犬的常规治疗中添加的短期效果。在一项前瞻性、随机、单盲研究中，5只患有C期MMVD的狗接受了达格列净（0.31 mg/kg， PO, q24h）和匹莫苯丹、呋塞米和雷米普利的联合治疗。对照组（n = 7）给予不含达格列净的常规治疗。在基线（D0）和随访（D28、D84和D140）收集超声心动图、心电图、血压、血糖、NT-proBNP和尿糖数据。与对照组相比，接受达格列净治疗的狗左心房与主动脉根比、左心室舒张内径与体重、舒张末期容积、舒张末期容积指数、收缩期末期容积和收缩期末期容积指数从基线变化的百分比显著降低（P < 0.05）。此外，达格列净组的射血分数显著升高（P < 0.05）。只有达格列净组持续出现血糖升高。这些发现表明，在常规治疗中加入达格列净可能会促进C期MMVD犬的逆转重构并改善心功能。进一步的研究需要更大的样本量和更长的随访时间来验证达格列净对兽医患者的心脏保护作用。

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引用次数: 0

Effect of dietary wine lees on oxidative stress, egg quality and yolk lipid stability in laying hens fed with a diet rich in n-3 polyunsaturated fats 饲粮中添加酒糟对饲粮富含n-3多不饱和脂肪蛋鸡氧化应激、蛋品质和蛋黄脂质稳定性的影响

IF 1.9 Q2 AGRICULTURE, DAIRY & ANIMAL SCIENCE

Veterinary and Animal Science

Pub Date : 2025-09-15 DOI: 10.1016/j.vas.2025.100509

Daniel Mierlita , Stelian Dărăban , Adrian Macri , Ioan Mircea Pop

Wine lees (WLs) are a by-product of the winemaking process, but the valorization of WLs as poultry feed has not yet been achieved. Accordingly, the aim of the present study was to evaluate the potential benefits of using WLs as a feed ingredient. To this end, we investigated their effects on serum and hepatic markers of oxidative stress and egg quality in laying hens susceptible to lipid peroxidation by feeding them diets enriched in n-3 PUFA. In addition, lipid profile and oxidative stability of the yolk during storage were evaluated. A total of 128 30-week-old Tetra SL laying hens were divided into four groups (n = 32): the negative control (C–) received a standard diet, while the three other groups (positive control, C+; WL2.5; and WL5) received the standard diet supplemented with 5 % linseed oil rich in n-3 PUFA. In the WL2.5 and WL5 groups, WLs were introduced into the diet at levels of 2.5 % and 5 %, respectively. The increased intake of n-3 PUFA induced oxidative stress, which was evidenced by increased malondialdehyde (MDA) and reduced serum and liver antioxidant enzyme activity. However, these parameters were significantly improved in hens supplemented with WLs. The reduction in n-3 PUFA content in stored eggs was < 19 % in hens fed with WLs, while the reduction exceeded 33 % in the C+ group. In conclusion, WLs can improve egg production and egg quality (yolk color and cholesterol content), the antioxidant status of hens and the oxidative stability of eggs, with the possibility of extending their shelf life.

酒渣（WLs）是酿酒过程的副产品，但其作为家禽饲料的价值尚未实现。因此，本研究的目的是评估使用wl作为饲料原料的潜在效益。为此，我们研究了饲粮中添加n-3 PUFA对脂质过氧化易感蛋鸡血清和肝脏氧化应激指标及蛋品质的影响。此外，还对贮藏期间蛋黄的脂质特征和氧化稳定性进行了评价。选取30周龄利乐SL蛋鸡128只，随机分为4组（n = 32）：阴性对照组（C -组）饲喂标准饲粮，阳性对照组（C +组）、WL2.5组和WL5组（WL5组）饲喂在标准饲粮中添加5%富含n-3 PUFA的亚麻籽油的饲粮。在WL2.5和WL5组，饲粮中分别添加2.5%和5%的wl。n-3 PUFA摄入增加可诱导氧化应激，其表现为丙二醛（MDA）升高，血清和肝脏抗氧化酶活性降低。然而，在添加WLs的母鸡中，这些参数显著提高。添加WLs的母鸡贮藏蛋中n-3 PUFA含量降低了19%，而添加C+的母鸡贮藏蛋中n-3 PUFA含量降低超过33%。综上所述，添加WLs可以提高蛋鸡的产蛋量和蛋品质（蛋黄颜色和胆固醇含量），提高母鸡的抗氧化能力和鸡蛋的氧化稳定性，并有可能延长鸡蛋的保质期。

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引用次数: 0

Infrared thermographic imaging as a promising on-farm method to estimate parasite load in dairy goats 红外热成像作为一种很有前途的农场方法来估计奶山羊的寄生虫负荷

IF 1.9 Q2 AGRICULTURE, DAIRY & ANIMAL SCIENCE

Veterinary and Animal Science

Pub Date : 2025-09-12 DOI: 10.1016/j.vas.2025.100506

M. Bernau , T. Schilling , H. Eßlinger , L.E. Hoelzle , S.A. Goth

Faecal egg count (FEC) evaluation is the method of choice to detect parasite load in individuals, but it cannot be used directly on farm. Clinical parameters like body condition scores (BCS), body weight (BW) and milk yield (MY) have been described in this context, but all fail due to a low correlation with FEC. The present study evaluated the utility of clinical parameters, linear body measurements, and infrared thermographic imaging (IRT) in eight dairy goat herds in Germany as potential methods for estimating FEC categories. A total of 893 dairy goats comprising German Fawn Goats (GFG) and German White Goats (GWG) were examined up to six times during three consecutive lactation periods. FEC was determined using individual faecal samples, analysed via the McMaster method. Based on eggs per gram of faeces (EPG), mean FEC across all examinations was categorized into three groups: low (Category 1: ≤500 EPG), medium (Category 2: >500 to ≤1500 EPG), and high (Category 3: >1500 EPG). Statistical analyses were conducted using SAS Studio to identify factors influencing FEC. Significant differences were observed between the two breeds.

In GFG, animals in Category 3 exhibited significantly lower BCS, BW, chest circumference, and MY compared to those in Category 1. IRT measurements also showed significant differences across FEC categories, with higher IRT values observed in goats with higher FEC. These findings suggest that IRT has potential as a non-invasive, on-farm method for assessing parasitic load. However, further studies are necessary to validate its practical application as a direct health monitoring method.

粪卵计数（FEC）评估是检测个体寄生虫负荷的首选方法，但不能直接在农场使用。临床参数，如身体状况评分（BCS），体重（BW）和产奶量（MY）已经在这方面进行了描述，但由于与FEC的相关性较低，这些参数都失败了。本研究评估了临床参数、线性身体测量和红外热成像（IRT）在德国8个奶山羊群中的效用，作为估计FEC类别的潜在方法。共有893只奶山羊，包括德国小鹿山羊（GFG）和德国白山羊（GWG），在连续三个哺乳期进行了多达6次的检查。利用个体粪便样本测定FEC，并通过麦克马斯特方法进行分析。根据每克粪便的卵数（EPG），所有检查的平均FEC分为三组：低（第一类：≤500 EPG），中（第二类：>；500至≤1500 EPG）和高（第三类：>；1500 EPG）。采用SAS Studio进行统计分析，确定影响FEC的因素。两个品种之间存在显著差异。在GFG中，与第1类动物相比，第3类动物的BCS、体重、胸围和MY明显较低。IRT测量也显示出FEC类别之间的显著差异，在FEC较高的山羊中观察到较高的IRT值。这些发现表明，IRT有潜力作为一种非侵入性的农场寄生负荷评估方法。然而，需要进一步的研究来验证其作为直接健康监测方法的实际应用。

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引用次数: 0

Antimicrobial resistance and biofilm-forming ability in Staphylococcus aureus causing clinical bovine mastitis in Chitwan, Nepal 在尼泊尔奇旺引起临床牛乳腺炎的金黄色葡萄球菌的抗微生物药物耐药性和生物膜形成能力

IF 1.9 Q2 AGRICULTURE, DAIRY & ANIMAL SCIENCE

Veterinary and Animal Science

Pub Date : 2025-09-12 DOI: 10.1016/j.vas.2025.100508

Sujan Adhikari , Swagat Khanal , Aakash Marasini , Prakash Panthi , Akash Adhikari , Himal Luitel , Yagya Raj Pandeya

This cross-sectional study, conducted from August to December 2024, investigated the antimicrobial resistance and biofilm-forming abilities of Staphylococcus aureus from clinical bovine mastitis in Chitwan, Nepal. Out of 134 California Mastitis Test-positive milk samples, 32 (23.9%) were confirmed as S. aureus by biochemical tests and species-specific nuc gene PCR. Antimicrobial susceptibility testing of the 32 isolates against 12 antibiotics revealed high resistance rates, particularly to ampicillin (78.1%), nalidixic acid (75.0%), and enrofloxacin (62.5%). The prevalence of multidrug resistance (MDR), defined as resistance to ≥3 antibiotic classes, was alarmingly high, with 27 (84.4%) isolates classified as MDR. Presumptive methicillin-resistant S. aureus (MRSA), detected via cefoxitin resistance, was identified in 14 (43.8%) isolates, all of which were also MDR. Biofilm-forming abilities were assessed qualitatively and quantitatively, with 5 (15.6%) isolates classified as strong biofilm producers. Fisher’s exact test revealed no significant association between biofilm formation and overall MDR status (p > 0.05). However, a statistically significant correlation was found between strong biofilm formation and high-level MDR (resistance to ≥ 4 classes) (p < 0.05), as well as between strong biofilm formation and presumptive MRSA status (p < 0.01). These findings highlight the co-existence of high-level resistance and strong virulence phenotypes in S. aureus from bovine mastitis in Nepal, underscoring the urgent need for robust antimicrobial stewardship, enhanced surveillance, and the development of strategies to mitigate biofilm-associated treatment failures.

这项横断面研究于2024年8月至12月进行，调查了尼泊尔Chitwan临床牛乳腺炎金黄色葡萄球菌的抗微生物药物耐药性和生物膜形成能力。134份加州乳腺炎试验阳性乳样中，经生化试验和种特异性nuc基因PCR鉴定为金黄色葡萄球菌32份（23.9%）。32株菌株对12种抗生素的药敏试验显示，耐药率较高，其中对氨苄西林（78.1%）、萘啶酸（75.0%）和恩诺沙星（62.5%）耐药率最高。多药耐药（MDR）（定义为对≥3种抗生素的耐药）的患病率高得惊人，有27株（84.4%）被列为MDR。通过对头孢西丁的耐药检测，在14株（43.8%）菌株中发现疑似耐甲氧西林金黄色葡萄球菌（MRSA），均为耐多药。定性和定量评估了生物膜形成能力，其中5株（15.6%）被列为强生物膜生产者。Fisher精确检验显示生物膜形成与总体MDR状态之间无显著关联（p > 0.05）。然而，强生物膜形成与高水平MDR（≥4类耐药）之间存在统计学显著相关性（p < 0.05），强生物膜形成与推定MRSA状态之间存在统计学显著相关性（p < 0.01）。这些发现强调了来自尼泊尔牛乳腺炎的金黄色葡萄球菌的高耐药性和强毒力表型共存，强调了迫切需要强有力的抗菌药物管理、加强监测和制定策略以减轻生物膜相关治疗失败。

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引用次数: 0

Robust BICP0-gB indirect ELISA for the accurate diagnosis of bovine alphaherpesvirus 1 infections 准确诊断牛甲疱疹病毒1型感染的强有力的BICP0-gB间接ELISA方法

IF 1.9 Q2 AGRICULTURE, DAIRY & ANIMAL SCIENCE

Veterinary and Animal Science

Pub Date : 2025-09-10 DOI: 10.1016/j.vas.2025.100507

Sen Zhang , Liyuan Song , Xin Yin , Changmin Hu , Yingyu Chen , Aizhen Guo

Bovine alphaherpesvirus 1 (BoHV-1) represents a significant threat to the cattle industry, emphasizing the need for reliable diagnostic tools that facilitate effective disease management. Current diagnostic methods, including virus neutralization tests (VNTs), are often complex and labor intensive, but existing antibody detection assays may lack sufficient accuracy. In this study, we developed a novel indirect enzyme-linked immunosorbent assay (iELISA) utilizing both BICP0 and gB proteins to enhance the detection of BoHV-1 infections. The inclusion of BICP0, a pivotal protein during the early stages of viral infection, markedly improved the assay's specificity and sensitivity. The BICP0-gB iELISA exhibited a high degree of concordance with the VNT, demonstrating superior sensitivity and specificity. Preliminary clinical evaluations revealed a real prevalence of 40.3 % (95 % CI: 33.1 %-48.0 %) in serum samples from yaks in Qinghai Province, which aligns with the documented prevalence of BoHV-1 in the region. These results underscore that BICP0-gB iELISA is a robust and reliable diagnostic tool for the differential diagnosis of BoHV-1, providing a cost-effective and efficient solution for high-throughput screening in the livestock industry.

牛甲疱疹病毒1型（BoHV-1）对养牛业构成重大威胁，强调需要可靠的诊断工具，促进有效的疾病管理。目前的诊断方法，包括病毒中和试验（vnt），通常是复杂和劳动密集型的，但现有的抗体检测分析可能缺乏足够的准确性。在这项研究中，我们开发了一种新的间接酶联免疫吸附试验（iELISA），利用BICP0和gB蛋白来增强BoHV-1感染的检测。BICP0是病毒感染早期的一种关键蛋白，它的加入显著提高了检测的特异性和敏感性。BICP0-gB iELISA与VNT高度一致，具有较高的敏感性和特异性。初步临床评估显示，青海省牦牛血清样本的实际流行率为40.3% (95% CI: 33.1% - 48.0%)，这与该地区记录的BoHV-1流行率一致。这些结果表明，BICP0-gB iELISA是一种稳健可靠的BoHV-1鉴别诊断工具，为畜牧业高通量筛查提供了一种经济高效的解决方案。

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引用次数: 0

Role of triptorelin and single fixed-time artificial insemination on productive and reproductive performance of hyperprolific sows 雷公藤素和单次定时人工授精对高产母猪生产和繁殖性能的影响

IF 1.9 Q2 AGRICULTURE, DAIRY & ANIMAL SCIENCE

Veterinary and Animal Science

Pub Date : 2025-08-30 DOI: 10.1016/j.vas.2025.100505

María Victoria Falceto , David Reyero , María Teresa Tejedor , Paul Renaud , Ana Mateu , Antonio Lafuente , Cristina Bonastre , Alicia Laborda , Ana Maria Garrido , Olga Mitjana

Single fixed-time artificial insemination (SFTAI) provides a streamlined approach to improving reproductive management. We evaluated the impact of incorporating triptorelin acetate (GnRH agonist) into a SFTAI protocol on a commercial farm. Hyperprolific sows not in estrus by day 4 post-weaning were randomly assigned to two groups. Group-1 (G1; n = 243) received no triptorelin treatment and daily post-cervical artificial inseminations (PCAI) based on estrus detection (2.59±0.034 inseminations/sow). Group-2 (G2; n = 249) received a single intravaginal dose of triptorelin (0.2 mg) at 96 ± 2 h post-weaning, then one PCAI 22 ± 2 h later. No significant differences (P > 0.05) between groups in conception rate (G1: 98.8 %; G2: 97.6 %), farrowing rate (G1:95.1 %; G2: 94.4 %) or litter characteristics (total born, live-, stillborn and mummified piglets). Both groups showed similar delivery batch durations (P = 0.414). Distribution of deliveries during the delivery period was similar (P = 0.455). Induction of labor was need more frequently in G1 (P < 0.001). G2 had a higher proportion of sows with shorter gestations (112–115 days: 166/235, 70.6 %) compared to G1 (112–115 days: 82/231; 35.5 %, P < 0.001). A significantly higher proportion of G2 piglets received longer lactation (27 - 33 days) compared to G1 (2210/3023=73.1 % vs 2774/4395=63.1 %; P = 0.032). G1 had a higher proportion of low-weight piglets (≤4.0 kg; 716/43950= 16.3 % vs.435/3023= 14.4 %; while G2 had more heavy piglets (≥6.5 kg; 1228/3023=40.5 % vs.1675/4395= 38.1 %) (P = 0.027). Observed differences in lactation length could be responsible for these weaning weight differences, rather than triptorelin treatment itself. This protocol did not worsen productive and reproductive performances.

单次定时人工授精（SFTAI）为改善生殖管理提供了一种简化的方法。我们在一个商业农场评估了将雷公藤雷素醋酸酯（GnRH激动剂）纳入SFTAI方案的影响。断奶后第4天未发情的高产母猪随机分为两组。1组（G1, n = 243）不给予雷公霉素治疗，每日根据发情检测进行宫颈后人工授精（PCAI）（2.59±0.034次授精/头）。2组（G2, n = 249）在断奶后96±2小时接受单次阴道内给药（0.2 mg）， 22±2小时后接受1次PCAI。各组受胎率（G1: 98.8%; G2: 97.6%）、产仔率（G1: 95.1%; G2: 94.4%）和产仔特征（出生仔猪、活仔猪、死胎仔猪和干尸仔猪总数）均无显著差异（P > 0.05）。两组的交付批次持续时间相似（P = 0.414）。分娩期间的分娩分布相似（P = 0.455）。G1期需要引产的频率更高（P < 0.001）。G1组（112-115 d: 82/231; 35.5%, P < 0.001）较G1组（112-115 d: 82/231, P < 0.001）妊娠期较短的母猪比例较高（112-115 d: 166/235, 70.6%）。与G1相比，G2仔猪泌乳时间较长（27 - 33 d）的比例显著高于G1 （2210/3023= 73.1% vs 2774/4395= 63.1%; P = 0.032）。G1组低重仔猪比例较高（≤4.0 kg, 716/43950= 16.3% vs.435/3023= 14.4%）， G2组重重仔猪比例较高（≥6.5 kg, 1228/3023= 40.5% vs.1675/4395= 38.1%）（P = 0.027）。观察到的哺乳期长度的差异可能是导致断奶体重差异的原因，而不是雷普妥林治疗本身。该方案不影响生产和繁殖性能。

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引用次数: 0

Effect of encapsulated Averrhoa carambola L. leaf extract on the growth performance, haemato-biochemical parameters, gut health, and antioxidant activity in broiler chickens 包封杨桃叶提取物对肉鸡生长性能、血液生化指标、肠道健康和抗氧化活性的影响

IF 1.9 Q2 AGRICULTURE, DAIRY & ANIMAL SCIENCE

Veterinary and Animal Science

Pub Date : 2025-08-29 DOI: 10.1016/j.vas.2025.100504

Binti Ma’rifah, Edjeng Suprijatna, Dwi Sunarti, Luthfi Djauhari Mahfudz, Sri Kismiati, Teysar Adi Sarjana, Rina Muryani, Hanna Dzawish Shihah, Nur Maulida Wahyuni, Sugiharto Sugiharto

This study aimed to evaluate the effect of encapsulated Averrhoa carambola L. leaf extract (EAC) as a phytobiotic feed additive on the growth performance, haemato-biochemical parameters, gut health, and antioxidant activity in broiler chickens. A total of 280 one-day-old chicks were allocated to four treatments with seven replicates and 10 broilers per replicate (CON: control group/basal diet; EAC1: basal diet supplemented with 500 mg/kg EAC; EAC2: basal diet supplemented with 1000 mg/kg EAC; EAC3: basal diet supplemented with 1500 mg/kg EAC. Growth performance was significantly enhanced (P < 0.05), as evidenced by increased body weight gain (days 15–21) and feed efficiency (days 15–35) in all treatment group compared with the control group. Haemato-biochemical parameters were positively influenced, with leukocytes and lymphocyte levels being significantly lower (P < 0.05) in the EAC2 group than in the control group. The uric acid concentration was significantly reduced (P < 0.05) in EAC1 and EAC3 groups compared to the control group. Gut health indicator improved, including the relative weight of the small intestine, liver, abdominal fat, length of duodenum and jejunum, weight and length ratio of the jejunum and ileum, ileum crypt depth, and lactic acid bacteria count in the ileum, were significantly higher (P < 0.05) in the EAC1 group than in the others groups. In conclusion, the encapsulated Averrhoa carambola L. leaf extract is beneficial for enhancing growth performance, haemato-biochemicals parameters, and gut health in broiler chickens.

本试验旨在研究杨桃叶提取物（EAC）作为植物性饲料添加剂对肉鸡生长性能、血液生化指标、肠道健康和抗氧化活性的影响。选取280只1日龄肉鸡，分为4个处理，每处理7个重复，每个重复10只鸡（CON：对照组/基础饲粮；EAC1：基础饲粮中添加500 mg/kg EAC； EAC2：基础饲粮中添加1000 mg/kg EAC； EAC3：基础饲粮中添加1500 mg/kg EAC）。与对照组相比，各处理组的增重（15-21 d）和饲料效率（15-35 d）均显著提高了生长性能（P < 0.05）。血液生化指标受到积极影响，白细胞和淋巴细胞水平显著低于对照组（P < 0.05）。与对照组相比，EAC1和EAC3组尿酸浓度显著降低（P < 0.05）。肠道健康指标，包括小肠相对重量、肝脏相对重量、腹部脂肪、十二指肠和空肠长度、空肠与回肠重量与长度比、回肠隐窝深度、回肠乳酸菌数量，EAC1组显著高于其他各组（P < 0.05）。综上所述，包被杨桃叶提取物有利于提高肉鸡的生长性能、血液生化指标和肠道健康。

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引用次数: 0