Veterinary and Animal Science最新文献_第4页

Deciphering autosomal and X-linked genetic effects of early growth traits in Murciano-Granadina goats via a multivariate animal model 通过多变量动物模型解读穆尔西亚诺-格拉纳迪纳山羊早期生长性状的常染色体和x连锁遗传效应

IF 1.9 Q2 AGRICULTURE, DAIRY & ANIMAL SCIENCE

Veterinary and Animal Science

Pub Date : 2026-03-01 Epub Date: 2025-12-07 DOI: 10.1016/j.vas.2025.100553

Jamshid Ehsaninia , Mohammad Bagher Zandi , Moein Taned , Alireza Bagheripour

This study aimed to quantify autosomal and sex-linked genetic parameters for early growth traits in the Murciano-Granadina goat breed. Data were collected from a commercial dairy herd in Ghaleh-Ganj, southern Iran. A total of 19,582 records for birth weight (BWT) and 9157 records for weaning weight (WWT) were analyzed, covering animals born between 2016 and 2023, descended from 460 bucks and 5382 does. Live weight measurements were used to calculate preweaning growth rate (PWGR), preweaning Kleiber ratio (PWKR), and preweaning growth efficiency (PWGE). Environmental effects were evaluated using aov package in R, and the optimal multivariate model was constructed by combining the best-fitting univariate models for each trait. Genetic parameters were estimated using this optimal multivariate animal model and the average information restricted maximum likelihood algorithm in WOMBAT software. Estimated direct autosomal heritabilities (

h_{a}^{2}

) were 0.05 for BWT, 0.08 for WWT, 0.06 for PWGR and PWGE, and 0.09 for PWKR. Corresponding direct sex-linked heritabilities (

h_{s}^{2}

) were 0.03 for BWT, 0.02 for WWT, 0.01 for PWGR, and PWKR, and 0.04 for PWGE. Excluding sex-linked chromosomal effects from optimal model led to a 3–14 % increase in autosomal additive genetic variance and a 7–20 % increase in the residual variance. Autosomal genetic correlations (r_a) ranged from -0.70 (BWT-PWGE) to 0.83 (PWKR-PWGR), while sex-linked genetic correlations (r_s) ranged from -0.75 (BWT-PWGE) to 0.93 (WWT-PWGE). These findings highlight the relevance of accounting for sex-linked additive inheritance in genetic evaluations, supporting more informed selection decisions and contributing to improved genetic progress in Murciano-Granadina goats.

本研究旨在量化Murciano-Granadina山羊品种早期生长性状的常染色体和性别连锁遗传参数。数据收集自伊朗南部Ghaleh-Ganj的一个商业奶牛群。研究人员共分析了出生体重（BWT）的19582条记录和断奶体重（WWT）的9157条记录，涵盖了2016年至2023年出生的动物，从460头公鹿和5382头公鹿下降。测定活重计算断奶前生长率（PWGR）、断奶前克莱伯比（PWKR）和断奶前生长效率（PWGE）。利用R中的aov包对环境效应进行评价，将各性状的最优拟合单变量模型组合，构建最优多变量模型。利用该最优多元动物模型和平均信息限制最大似然算法在WOMBAT软件中估计遗传参数。估计BWT的直接常染色体遗传率（ha2）为0.05，WWT为0.08，PWGR和PWGE为0.06，PWKR为0.09。相应的直接性连锁遗传力（hs2）分别为：BWT为0.03，WWT为0.02，PWGR和PWKR为0.01，PWGE为0.04。从最优模型中排除性别连锁染色体效应导致常染色体加性遗传变异增加3 - 14%，剩余变异增加7 - 20%。常染色体遗传相关性（ra）范围从-0.70 （BWT-PWGE）到0.83 (PWKR-PWGR)，而性别相关遗传相关性（rs）范围从-0.75 （BWT-PWGE）到0.93 （WWT-PWGE）。这些发现强调了在遗传评估中考虑性别连锁加性遗传的相关性，支持了更明智的选择决策，并有助于改善穆尔西亚诺-格拉纳迪纳山羊的遗传进展。

{"title":"Deciphering autosomal and X-linked genetic effects of early growth traits in Murciano-Granadina goats via a multivariate animal model","authors":"Jamshid Ehsaninia , Mohammad Bagher Zandi , Moein Taned , Alireza Bagheripour","doi":"10.1016/j.vas.2025.100553","DOIUrl":"10.1016/j.vas.2025.100553","url":null,"abstract":"<div><div>This study aimed to quantify autosomal and sex-linked genetic parameters for early growth traits in the Murciano-Granadina goat breed. Data were collected from a commercial dairy herd in Ghaleh-Ganj, southern Iran. A total of 19,582 records for birth weight (BWT) and 9157 records for weaning weight (WWT) were analyzed, covering animals born between 2016 and 2023, descended from 460 bucks and 5382 does. Live weight measurements were used to calculate preweaning growth rate (PWGR), preweaning Kleiber ratio (PWKR), and preweaning growth efficiency (PWGE). Environmental effects were evaluated using <em>aov</em> package in R, and the optimal multivariate model was constructed by combining the best-fitting univariate models for each trait. Genetic parameters were estimated using this optimal multivariate animal model and the average information restricted maximum likelihood algorithm in WOMBAT software. Estimated direct autosomal heritabilities (<span><math><msubsup><mi>h</mi><mrow><mi>a</mi></mrow><mn>2</mn></msubsup></math></span>) were 0.05 for BWT, 0.08 for WWT, 0.06 for PWGR and PWGE, and 0.09 for PWKR. Corresponding direct sex-linked heritabilities (<span><math><msubsup><mi>h</mi><mrow><mi>s</mi></mrow><mn>2</mn></msubsup></math></span>) were 0.03 for BWT, 0.02 for WWT, 0.01 for PWGR, and PWKR, and 0.04 for PWGE. Excluding sex-linked chromosomal effects from optimal model led to a 3–14 % increase in autosomal additive genetic variance and a 7–20 % increase in the residual variance. Autosomal genetic correlations (r<sub>a</sub>) ranged from -0.70 (BWT-PWGE) to 0.83 (PWKR-PWGR), while sex-linked genetic correlations (r<sub>s</sub>) ranged from -0.75 (BWT-PWGE) to 0.93 (WWT-PWGE). These findings highlight the relevance of accounting for sex-linked additive inheritance in genetic evaluations, supporting more informed selection decisions and contributing to improved genetic progress in Murciano-Granadina goats.</div></div>","PeriodicalId":37152,"journal":{"name":"Veterinary and Animal Science","volume":"31 ","pages":"Article 100553"},"PeriodicalIF":1.9,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145738705","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effect of the high dietary inclusion of wheat dried distillers’ grains with solubles with and without exogenous enzyme supplementation in broiler diet on performance, litter quality and certain animal welfare issues 添加和不添加外源酶时，高添加小麦干酒糟可溶物对肉鸡生产性能、产仔质量和某些动物福利的影响

IF 1.9 Q2 AGRICULTURE, DAIRY & ANIMAL SCIENCE

Veterinary and Animal Science

Pub Date : 2026-03-01 Epub Date: 2025-12-15 DOI: 10.1016/j.vas.2025.100557

Márk Tóth , Yazavinder Singh , Krisztián Balogh , Erika Zándoki , Szabina Kulcsár , Benjámin Kövesi , Zsolt Ancsin , Tibor Pap , Miklós Mézes , Mária Kovács-Weber , Márta Erdélyi

The study assessed the effects of high dietary inclusion of wheat dried distillers’ grains with solubles (WDDGS), with or without a non-starch polysaccharide (NSP) degrading enzyme (Enzyme) supplement, on broiler performance, carcass traits, meat quality, litter condition, and footpad dermatitis (FPD). Three diets: Control, 30 % WDDGS, and 30 % WDDGS+Enzyme, were fed to 312 Cobb 500 broilers over 42 days. From day 7 onward, WDDGS and Enzyme groups exhibited significantly higher body weight (BW) and weight gain (WG) compared to Control (P < 0.001), with no differences in feed conversion ratio (FCR) or mortality. At day 42, slaughter, carcass, breast, and leg weights increased in WDDGS groups vs Control (P < 0.001), while relative yields remained unchanged. Breast meat pH, water-holding capacity (WHC), shear force, and proximate composition did not differ among treatments; however, meat yellowness (b*) decreased in WDDGS groups (P < 0.001). Litter moisture and FPD scores rose in the WDDGS group (P < 0.001), but enzyme supplementation partially mitigated these welfare concerns, reducing both parameters though not to Control levels. These findings indicate that diets containing 30 % WDDGS can enhance growth performance and carcass weights without compromising meat quality, but may impair litter conditions and increase FPD incidence. Supplementation with NSP-degrading enzymes did not affect production or meat characteristics but improved litter moisture and reduced FPD occurrence. The nutritional feasibility of high-level WDDGS inclusion is confirmed; future research should address gut health, nutrient digestibility, and commercial-scale applications.

本试验旨在评价饲粮中添加或不添加非淀粉多糖（NSP）降解酶（酶）对肉仔鸡生产性能、胴体性状、肉品质、窝产状况和脚垫皮炎（FPD）的影响。选取312只科布500肉鸡，饲喂对照、30% WDDGS和30% WDDGS+酶3种饲粮，试验期42 d。从第7天开始，WDDGS组和酶组的体重（BW）和增重（WG）显著高于对照组（P < 0.001），饲料系数和死亡率无显著差异。第42天，WDDGS组的屠宰重量、胴体重量、胸重和腿重均高于对照组（P < 0.001），而相对产量保持不变。不同处理胸脯肉的pH值、持水量、剪切力和近似成分没有差异；WDDGS组肉黄度（b*）降低（P < 0.001）。WDDGS组凋落物水分和FPD评分上升（P < 0.001），但酶补充部分缓解了这些福利问题，降低了这两个参数，但没有达到控制水平。上述结果表明，饲粮中添加30% WDDGS可以在不影响肉品质的情况下提高生长性能和胴体重，但可能会损害产仔条件并增加FPD发生率。补充nsp降解酶不会影响产量或肉质特性，但会改善凋落物水分，减少FPD的发生。证实了高水平WDDGS纳入的营养可行性；未来的研究应着眼于肠道健康、营养物质消化率和商业规模应用。

{"title":"Effect of the high dietary inclusion of wheat dried distillers’ grains with solubles with and without exogenous enzyme supplementation in broiler diet on performance, litter quality and certain animal welfare issues","authors":"Márk Tóth , Yazavinder Singh , Krisztián Balogh , Erika Zándoki , Szabina Kulcsár , Benjámin Kövesi , Zsolt Ancsin , Tibor Pap , Miklós Mézes , Mária Kovács-Weber , Márta Erdélyi","doi":"10.1016/j.vas.2025.100557","DOIUrl":"10.1016/j.vas.2025.100557","url":null,"abstract":"<div><div>The study assessed the effects of high dietary inclusion of wheat dried distillers’ grains with solubles (WDDGS), with or without a non-starch polysaccharide (NSP) degrading enzyme (Enzyme) supplement, on broiler performance, carcass traits, meat quality, litter condition, and footpad dermatitis (FPD). Three diets: Control, 30 % WDDGS, and 30 % WDDGS+Enzyme, were fed to 312 Cobb 500 broilers over 42 days. From day 7 onward, WDDGS and Enzyme groups exhibited significantly higher body weight (BW) and weight gain (WG) compared to Control (<em>P</em> < 0.001), with no differences in feed conversion ratio (FCR) or mortality. At day 42, slaughter, carcass, breast, and leg weights increased in WDDGS groups vs Control (<em>P</em> < 0.001), while relative yields remained unchanged. Breast meat pH, water-holding capacity (WHC), shear force, and proximate composition did not differ among treatments; however, meat yellowness (<em>b</em>*) decreased in WDDGS groups (<em>P</em> < 0.001). Litter moisture and FPD scores rose in the WDDGS group (<em>P</em> < 0.001), but enzyme supplementation partially mitigated these welfare concerns, reducing both parameters though not to Control levels. These findings indicate that diets containing 30 % WDDGS can enhance growth performance and carcass weights without compromising meat quality, but may impair litter conditions and increase FPD incidence. Supplementation with NSP-degrading enzymes did not affect production or meat characteristics but improved litter moisture and reduced FPD occurrence. The nutritional feasibility of high-level WDDGS inclusion is confirmed; future research should address gut health, nutrient digestibility, and commercial-scale applications.</div></div>","PeriodicalId":37152,"journal":{"name":"Veterinary and Animal Science","volume":"31 ","pages":"Article 100557"},"PeriodicalIF":1.9,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145926459","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Comparative evaluation of milk yield and composition in three indigenous West African goat breeds under semi-intensive management 半集约管理下西非三个地方山羊品种的产奶量和成分比较评价

IF 1.9 Q2 AGRICULTURE, DAIRY & ANIMAL SCIENCE

Veterinary and Animal Science

Pub Date : 2026-03-01 Epub Date: 2025-12-18 DOI: 10.1016/j.vas.2025.100560

Helliot Kossi Dosseh , Sedo Eudes Anihouvi , Michel Kere , Rodrigue Vivien Cao Diogo , Mawule Sylvie Hounzangbé-Adoté , Luc Hippolyte Dossa

Milk production constitutes an essential component of livestock farming in Benin. Although goats are predominantly reared for meat, their milk represents a valuable nutritional alternative to cow’s milk, particularly for individuals with dietary sensitivities and children in food-insecure households. Nevertheless, despite the substantial goat population, caprine milk production remains significantly underexploited. This study assessed milk yield and composition of three indigenous West African goat breeds, Red Maradi Goat (RMG), West African Dwarf Goat (WAD), and West Long-Legged Goat (LLG), under semi-intensive systems in northern Benin to evaluate breed adaptability and support sustainable goat farming. Forty-eight goats (15 females and one male per breed) were selected from traditional farms and managed at an experimental station. Daily milk yield was recorded by hand-milking on days 8, 28, and 49 postpartum. A total of 318 milk samples were assessed for fat, protein, lactose, solids-not-fat (SNF), and pH using a Speedy Lab Milk Analyzer. The resulting data were then evaluated using Generalized Linear Mixed Models and correlation analysis. Milk yield differed significantly among breeds (p < 0.05), with RMG and LLG outperforming WAD. While WAD goats produced milk with significantly higher fat content, RMG maintained stable composition across lactation, WAD showed declines in most components except fat, and LLG exhibited increasing fat with decreasing SNF and density. Breed and lactation stage influenced milk pH, indicating physiological differences. Strong correlations emerged among SNF, protein, lactose, and density. These results position RMG and LLG as promising for yield and compositional stability, while WAD may be preferable for high-fat milk production. Selective breeding and improved feeding management could improve dairy productivity and sustainability in West African goat production systems.

牛奶生产是贝宁畜牧业的重要组成部分。虽然山羊主要用于肉类，但它们的奶是牛奶的一种有价值的营养替代品，特别是对饮食敏感的个人和粮食不安全家庭的儿童。然而，尽管山羊数量庞大，但羊奶产量仍未得到充分利用。本研究评估了贝宁北部半集约化体系下的三个本土西非山羊品种红马拉迪山羊（RMG）、西非矮山羊（WAD）和西长腿山羊（LLG）的产奶量和组成，以评估品种适应性并支持可持续山羊养殖。从传统农场选择48只山羊（每个品种15只母山羊和1只公山羊），在一个试验站进行管理。在产后第8、28和49天用手挤奶记录每日产奶量。使用Speedy Lab牛奶分析仪对318份牛奶样品进行脂肪、蛋白质、乳糖、非脂肪固体（SNF）和pH值的评估。然后使用广义线性混合模型和相关分析对所得数据进行评估。产奶量各品种间差异显著（p < 0.05）， RMG和LLG的产奶量优于WAD。WAD山羊产奶脂肪含量显著提高，RMG成分在整个哺乳期保持稳定，WAD山羊除脂肪外大部分成分呈下降趋势，LLG山羊脂肪含量随SNF和密度的降低而增加。品种和哺乳期影响牛奶pH值，显示出生理差异。SNF、蛋白质、乳糖和密度之间存在很强的相关性。这些结果表明RMG和LLG在产量和组成稳定性方面有希望，而WAD可能更适合高脂牛奶生产。选择性育种和改进饲养管理可以提高西非山羊生产系统的奶业生产力和可持续性。

{"title":"Comparative evaluation of milk yield and composition in three indigenous West African goat breeds under semi-intensive management","authors":"Helliot Kossi Dosseh , Sedo Eudes Anihouvi , Michel Kere , Rodrigue Vivien Cao Diogo , Mawule Sylvie Hounzangbé-Adoté , Luc Hippolyte Dossa","doi":"10.1016/j.vas.2025.100560","DOIUrl":"10.1016/j.vas.2025.100560","url":null,"abstract":"<div><div>Milk production constitutes an essential component of livestock farming in Benin. Although goats are predominantly reared for meat, their milk represents a valuable nutritional alternative to cow’s milk, particularly for individuals with dietary sensitivities and children in food-insecure households. Nevertheless, despite the substantial goat population, caprine milk production remains significantly underexploited. This study assessed milk yield and composition of three indigenous West African goat breeds, Red Maradi Goat (RMG), West African Dwarf Goat (WAD), and West Long-Legged Goat (LLG), under semi-intensive systems in northern Benin to evaluate breed adaptability and support sustainable goat farming. Forty-eight goats (15 females and one male per breed) were selected from traditional farms and managed at an experimental station. Daily milk yield was recorded by hand-milking on days 8, 28, and 49 postpartum. A total of 318 milk samples were assessed for fat, protein, lactose, solids-not-fat (SNF), and pH using a Speedy Lab Milk Analyzer. The resulting data were then evaluated using Generalized Linear Mixed Models and correlation analysis. Milk yield differed significantly among breeds (<em>p</em> < 0.05), with RMG and LLG outperforming WAD. While WAD goats produced milk with significantly higher fat content, RMG maintained stable composition across lactation, WAD showed declines in most components except fat, and LLG exhibited increasing fat with decreasing SNF and density. Breed and lactation stage influenced milk pH, indicating physiological differences. Strong correlations emerged among SNF, protein, lactose, and density. These results position RMG and LLG as promising for yield and compositional stability, while WAD may be preferable for high-fat milk production. Selective breeding and improved feeding management could improve dairy productivity and sustainability in West African goat production systems.</div></div>","PeriodicalId":37152,"journal":{"name":"Veterinary and Animal Science","volume":"31 ","pages":"Article 100560"},"PeriodicalIF":1.9,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145926509","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Complementary effects of probiotics and stimbiotics inclusion on carcass traits, meat quality and antioxidant activity in broiler chickens exposed to cyclic heat stress 循环热应激下添加益生菌和刺激菌对肉鸡胴体性状、肉品质和抗氧化活性的影响

IF 1.9 Q2 AGRICULTURE, DAIRY & ANIMAL SCIENCE

Veterinary and Animal Science

Pub Date : 2026-03-01 Epub Date: 2026-01-15 DOI: 10.1016/j.vas.2026.100575

R.M. Mokonyama , U. Marume , G. Moonsamy

This study assessed the complementary effects of probiotics and stimbiotics inclusion in diets on carcass traits and meat quality in broiler chickens exposed to cyclic heat stress. 600 day- old Cobb500 chicks were divided into five dietary groups; T1: Standard broiler diet, with no AGPs (NegControl); T2: Basal diet, with AGPs (PosControl); T3: Basal diet + 0.01% probiotics (Prob); T4: Basal diet + 0.01% stimbiotics (Stim) and T5: Basal diet + 0.01% (probiotics+ stimbiotics) (ProbStim). The birds were fed over a period of 42 days and then slaughtered for measurements of internal organs, carcass traits and meat quality attributes. The results revealed that dietary treatments had no effect on internal organs apart from the liver weight, duodenum and length of large intestine. Broiler chickens fed PosControl showed the heaviest liver, while those fed PosControl and ProbStim diets showed heavier duodenum weight. Carcass characteristics were not affected by diet apart from the wing weight. Broiler chickens fed Prob and ProbStim obtained heavier wing weight. With regards to meat quality, diet significantly (p < 0.05) affected the lightness (L*_i), yellowness (b*_i) and redness (a* _24hr). Diet also significantly influenced (p < 0.05) the antioxidant activity of the broiler meat on FRAP and TEAC assay. Prob and Stim individually showed high ferric ion reducing power, while on TEAC assay, NegControl and Prob showed the most efficient scavenger of radicals. The findings suggest that probiotics and stimbiotics can improve carcass traits and meat quality parameters in broilers while mitigating negative effects of heat stress.

本研究评估了饲粮中添加益生菌和刺激菌对循环热应激肉鸡胴体性状和肉品质的互补效应。600日龄Cobb500雏鸡分为5个饲粮组；T1：标准肉鸡日粮，不添加agp (NegControl)；T2：基础日粮，含AGPs (PosControl)；T3：基础日粮+ 0.01%益生菌（Prob）；T4：基础饲粮+ 0.01%的益生菌（Stim）和T5：基础饲粮+ 0.01%的益生菌+益生菌（ProbStim）。饲养42 d后屠宰，测定内脏器官、胴体性状和肉质性状。结果表明，饲粮处理对除肝脏重量、十二指肠和大肠长度外的其他脏器均无影响。饲粮饲喂PosControl的肉鸡肝脏重量最重，饲粮饲喂PosControl和ProbStim的肉鸡十二指肠重量最重。除翅重外，饲料对胴体性状无显著影响。饲喂Prob和ProbStim的肉鸡翅重较重。在肉质方面，饲粮显著（p < 0.05）影响了肉质的亮度（L*i）、黄度（b*i）和红度（a* 24hr）。在FRAP和TEAC试验中，饲粮对肉的抗氧化活性也有显著影响（p < 0.05）。Prob和Stim分别表现出较高的铁离子还原能力，而在TEAC试验中，NegControl和Prob表现出最有效的自由基清除剂。综上所述，饲粮中添加益生菌和刺激菌可以改善肉鸡胴体性状和肉品质参数，同时减轻热应激的负面影响。

{"title":"Complementary effects of probiotics and stimbiotics inclusion on carcass traits, meat quality and antioxidant activity in broiler chickens exposed to cyclic heat stress","authors":"R.M. Mokonyama , U. Marume , G. Moonsamy","doi":"10.1016/j.vas.2026.100575","DOIUrl":"10.1016/j.vas.2026.100575","url":null,"abstract":"<div><div>This study assessed the complementary effects of probiotics and stimbiotics inclusion in diets on carcass traits and meat quality in broiler chickens exposed to cyclic heat stress. 600 day- old Cobb500 chicks were divided into five dietary groups; T1: Standard broiler diet, with no AGPs (NegControl); T2: Basal diet, with AGPs (PosControl); T3: Basal diet + 0.01% probiotics (Prob); T4: Basal diet + 0.01% stimbiotics (Stim) and T5: Basal diet + 0.01% (probiotics+ stimbiotics) (ProbStim). The birds were fed over a period of 42 days and then slaughtered for measurements of internal organs, carcass traits and meat quality attributes. The results revealed that dietary treatments had no effect on internal organs apart from the liver weight, duodenum and length of large intestine. Broiler chickens fed PosControl showed the heaviest liver, while those fed PosControl and ProbStim diets showed heavier duodenum weight. Carcass characteristics were not affected by diet apart from the wing weight. Broiler chickens fed Prob and ProbStim obtained heavier wing weight. With regards to meat quality, diet significantly (<em>p</em> < 0.05) affected the lightness (<em>L</em>*<sub>i</sub>), yellowness (<em>b</em>*<sub>i</sub>) and redness (<em>a</em>* <sub>24hr</sub>). Diet also significantly influenced (<em>p</em> < 0.05) the antioxidant activity of the broiler meat on FRAP and TEAC assay. Prob and Stim individually showed high ferric ion reducing power, while on TEAC assay, NegControl and Prob showed the most efficient scavenger of radicals. The findings suggest that probiotics and stimbiotics can improve carcass traits and meat quality parameters in broilers while mitigating negative effects of heat stress.</div></div>","PeriodicalId":37152,"journal":{"name":"Veterinary and Animal Science","volume":"31 ","pages":"Article 100575"},"PeriodicalIF":1.9,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146037717","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Optimizing South African giraffe Epididymal sperm preservation techniques" 优化南非长颈鹿附睾精子保存技术

IF 1.9 Q2 AGRICULTURE, DAIRY & ANIMAL SCIENCE

Veterinary and Animal Science

Pub Date : 2026-03-01 Epub Date: 2026-01-19 DOI: 10.1016/j.vas.2026.100578

Ayanda Maqhashu , Willem Daffue , Charles Serrurier , Luther Mashilo , Edward Collin Albertyn , Francois Deacon

Giraffe populations have declined by 30–40% over the past three generations, resulting in local extinctions in many habitats. In contrast, the South African subspecies is stable and subject to culling in some reserves, creating opportunities for post-mortem gamete recovery to support conservation. This study evaluated the preservation potential of epididymal sperm collected from South African giraffe bulls. Paired testes from 12 bulls were collected post-mortem and placed at 5°C mobile fridge from the Sandveld Nature Reserve. Sperm was retrieved from the cauda epididymides by slicing immediately (n = 4), four hours (n = 4), six hours (n = 2), and eight hours post-mortem (n = 2). Samples were extended in tris-egg yolk (TEY) or Ark Biotechnology's proprietary flush buffer for liquid preservation at 4°C and cryopreservation. Sperm quality was assessed microscopically, and motility parameters were analyzed using two-way ANOVA. Sperm quality was maintained up to six hours post-mortem, with total motility exceeding 75%. A sharp decline occurred at eight hours (<20%). After 72 h at 4°C, Ark buffer preserved higher motility (62 ± 3.43%) than TEY (44 ± 3.21%). In contrast, TEY produced superior cryopreservation outcomes, with post-thaw motility of 70% immediately and 65 ± 2.55% after two hours, compared to Ark biotech buffer (25% and 0%, respectively). Epididymal sperm from giraffes can be effectively recovered up to six hours post-mortem without loss of quality. Ark Biotechnology's flush buffer is advantageous for short-term liquid preservation, while TEY is better suited for cryopreservation.

在过去的三代中，长颈鹿的数量下降了30-40%，导致许多栖息地的局部灭绝。相比之下，南非的亚种是稳定的，在一些保护区受到淘汰，为死后配子的恢复创造了机会，以支持保护。本研究评估了南非长颈鹿公牛附睾精子的保存潜力。12头公牛的成对睾丸在死后收集，并放置在Sandveld自然保护区的5°C移动冰箱中。通过立即（n = 4）、4小时（n = 4）、6小时（n = 2）和死后8小时（n = 2）切片从附睾尾提取精子。样品在三蛋黄（TEY）或Ark Biotechnology的专有冲洗缓冲液中延长，在4°C下进行液体保存和低温保存。显微镜下评估精子质量，并用双因素方差分析分析运动参数。精子质量在死后6小时内保持不变，总活动力超过75%。8小时时急剧下降（20%）。在4℃作用72 h后，Ark缓冲液的运动率（62±3.43%）高于TEY缓冲液（44±3.21%）。相比之下，与Ark生物技术缓冲液（分别为25%和0%）相比，TEY产生了更好的冷冻保存结果，解冻后立即运动性为70%，2小时后运动性为65±2.55%。长颈鹿的附睾精子可以在死后6小时内有效地恢复，而不会失去质量。Ark生物技术的冲洗缓冲液有利于短期液体保存，而TEY更适合冷冻保存。

{"title":"Optimizing South African giraffe Epididymal sperm preservation techniques\"","authors":"Ayanda Maqhashu , Willem Daffue , Charles Serrurier , Luther Mashilo , Edward Collin Albertyn , Francois Deacon","doi":"10.1016/j.vas.2026.100578","DOIUrl":"10.1016/j.vas.2026.100578","url":null,"abstract":"<div><div>Giraffe populations have declined by 30–40% over the past three generations, resulting in local extinctions in many habitats. In contrast, the South African subspecies is stable and subject to culling in some reserves, creating opportunities for post-mortem gamete recovery to support conservation. This study evaluated the preservation potential of epididymal sperm collected from South African giraffe bulls. Paired testes from 12 bulls were collected post-mortem and placed at 5°C mobile fridge from the Sandveld Nature Reserve. Sperm was retrieved from the cauda epididymides by slicing immediately (<em>n</em> = 4), four hours (<em>n</em> = 4), six hours (<em>n</em> = 2), and eight hours post-mortem (<em>n</em> = 2). Samples were extended in tris-egg yolk (TEY) or Ark Biotechnology's proprietary flush buffer for liquid preservation at 4°C and cryopreservation. Sperm quality was assessed microscopically, and motility parameters were analyzed using two-way ANOVA. Sperm quality was maintained up to six hours post-mortem, with total motility exceeding 75%. A sharp decline occurred at eight hours (<20%). After 72 h at 4°C, Ark buffer preserved higher motility (62 ± 3.43%) than TEY (44 ± 3.21%). In contrast, TEY produced superior cryopreservation outcomes, with post-thaw motility of 70% immediately and 65 ± 2.55% after two hours, compared to Ark biotech buffer (25% and 0%, respectively). Epididymal sperm from giraffes can be effectively recovered up to six hours post-mortem without loss of quality. Ark Biotechnology's flush buffer is advantageous for short-term liquid preservation, while TEY is better suited for cryopreservation.</div></div>","PeriodicalId":37152,"journal":{"name":"Veterinary and Animal Science","volume":"31 ","pages":"Article 100578"},"PeriodicalIF":1.9,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146037716","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Evaluation of the effects of microencapsulated DL-methionine on productive performance, immunity, plasma amino acids, and hepatic growth-related gene expression in broilers 微胶囊化dl -蛋氨酸对肉鸡生产性能、免疫力、血浆氨基酸和肝脏生长相关基因表达的影响

IF 1.9 Q2 AGRICULTURE, DAIRY & ANIMAL SCIENCE

Veterinary and Animal Science

Pub Date : 2026-03-01 Epub Date: 2026-02-06 DOI: 10.1016/j.vas.2026.100593

Mohammad Ali Khazab , Hossein Ali Ghasemi , Seyed Abdullah Hosseini , Iman Hajkhodadadi , Amir Hossein Alizadeh-Ghamsari

A 42-day feeding trial was conducted to compare a diet supplemented with crystalline DL-methionine (control) with diets containing microencapsulated DL-methionine (MEM) supplied at 60, 70, 80, 90, or 100 % of the commercially recommended level in broiler chickens. A total of 1260 one-day-old Arian broilers were randomly allotted to six treatments, with seven replicates of 30 birds each. Body weight gain (BWG), feed intake, and feed conversion ratio (FCR) did not differ between birds fed the control diet and those fed MEM diets containing ≥70 % of the recommended level, whereas the 60 % MEM diet reduced BWG and increased FCR. Increasing the dietary MEM level resulted in a quadratic increase in leg yield and a linear decrease in abdominal fat, without affecting hematological traits or leukocyte differential counts. The 100 % MEM diet increased influenza (H9N2) antibody titers, and all MEM levels enhanced Newcastle disease virus titers compared with the 60 % MEM diet; moreover, titers in the 100 % MEM group also exceeded those of the control. Plasma methionine, serine, and total amino acid concentrations were higher in birds fed the 60 % MEM diet than in those fed the 90 % MEM diet, while plasma cysteine showed a quadratic response as MEM level increased. Diets providing 80–100 % MEM upregulated hepatic GHR expression, and diets providing 90–100 % MEM also increased mTOR expression compared with the 60 % MEM diet. Overall, microencapsulated DL-methionine enabled approximately a 30 % reduction in supplemental methionine without impairing performance, while higher inclusion levels (≥80 % of the recommended level) beneficially modulated immune responses and growth-related gene expression.

本研究进行了为期42天的饲养试验，以比较添加结晶型dl -蛋氨酸（对照）的饲粮与添加微胶囊型dl -蛋氨酸（MEM）的饲粮（按商业推荐水平的60%、70%、80%、90%或100%供应）的肉鸡。试验选用1260只1日龄阿里乌尔肉鸡，随机分为6个处理，每7个重复，每个重复30只鸡。饲粮中MEM含量≥70%的对照组和饲粮中MEM含量≥70%的对照组的体增重（BWG）、采食量和饲料系数（FCR）无显著差异，60% MEM降低了BWG，提高了FCR。饲粮MEM水平的增加导致腿产量呈二次增长，腹部脂肪呈线性下降，但不影响血液学特征或白细胞差异计数。与60% MEM饲粮相比，100% MEM饲粮可提高流感（H9N2）抗体滴度，所有MEM水平均可提高新城疫病毒滴度；此外，100% MEM组的滴度也高于对照组。饲粮中MEM含量为60%的蛋氨酸、丝氨酸和总氨基酸浓度均高于饲粮中MEM含量为90%的蛋氨酸，血浆半胱氨酸随MEM水平的升高呈二次响应关系。与60% MEM饲粮相比，80 - 100% MEM饲粮上调了肝脏GHR表达，90 - 100% MEM饲粮也增加了mTOR表达。总体而言，微胶囊化dl -蛋氨酸可使蛋氨酸添加量减少约30%而不影响生产性能，而较高的包合水平（≥推荐水平的80%）有利于调节免疫反应和生长相关基因表达。

{"title":"Evaluation of the effects of microencapsulated DL-methionine on productive performance, immunity, plasma amino acids, and hepatic growth-related gene expression in broilers","authors":"Mohammad Ali Khazab , Hossein Ali Ghasemi , Seyed Abdullah Hosseini , Iman Hajkhodadadi , Amir Hossein Alizadeh-Ghamsari","doi":"10.1016/j.vas.2026.100593","DOIUrl":"10.1016/j.vas.2026.100593","url":null,"abstract":"<div><div>A 42-day feeding trial was conducted to compare a diet supplemented with crystalline DL-methionine (<strong>control</strong>) with diets containing microencapsulated DL-methionine (<strong>MEM</strong>) supplied at 60, 70, 80, 90, or 100 % of the commercially recommended level in broiler chickens. A total of 1260 one-day-old Arian broilers were randomly allotted to six treatments, with seven replicates of 30 birds each. Body weight gain (<strong>BWG</strong>), feed intake, and feed conversion ratio (<strong>FCR</strong>) did not differ between birds fed the control diet and those fed MEM diets containing ≥70 % of the recommended level, whereas the 60 % MEM diet reduced BWG and increased FCR. Increasing the dietary MEM level resulted in a quadratic increase in leg yield and a linear decrease in abdominal fat, without affecting hematological traits or leukocyte differential counts. The 100 % MEM diet increased influenza (H9N2) antibody titers, and all MEM levels enhanced Newcastle disease virus titers compared with the 60 % MEM diet; moreover, titers in the 100 % MEM group also exceeded those of the control. Plasma methionine, serine, and total amino acid concentrations were higher in birds fed the 60 % MEM diet than in those fed the 90 % MEM diet, while plasma cysteine showed a quadratic response as MEM level increased. Diets providing 80–100 % MEM upregulated hepatic GHR expression, and diets providing 90–100 % MEM also increased mTOR expression compared with the 60 % MEM diet. Overall, microencapsulated DL-methionine enabled approximately a 30 % reduction in supplemental methionine without impairing performance, while higher inclusion levels (≥80 % of the recommended level) beneficially modulated immune responses and growth-related gene expression.</div></div>","PeriodicalId":37152,"journal":{"name":"Veterinary and Animal Science","volume":"31 ","pages":"Article 100593"},"PeriodicalIF":1.9,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146188201","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Dietary supplementation of lignocellulose promotes the growth of Cherry Valley ducks by improving intestinal function 饲粮中添加木质纤维素可通过改善肠道功能促进樱桃谷鸭生长

IF 1.9 Q2 AGRICULTURE, DAIRY & ANIMAL SCIENCE

Veterinary and Animal Science

Pub Date : 2026-03-01 Epub Date: 2025-12-17 DOI: 10.1016/j.vas.2025.100558

Xinzhi Geng , Zhenzhen Chen , Jian Wang , Biao Dong , Jing Ge , Minmeng Zhao , Long Liu , Daoqing Gong , Haixia Liu , Tuoyu Geng

Dietary fiber contributes to improving intestinal morphology, barrier integrity, and microbial balance, thereby enhancing nutrient utilization and growth performance in animals. This study evaluated the effects of supplementing lignocellulose on intestinal function and growth performance in meat ducks. A total of 180 one-day-old Cherry Valley ducks were randomly assigned to the control group (CON), lignocellulose group 1 (LC1), and lignocellulose group 2 (LC2) (6 replicates each group, 10 ducks each replicate). Ducks in the CON group were fed a basal diet; the LC1 group received the basal diet supplemented with 0.6% lignocellulose from days 1 to 42, while the LC2 group received the basal diet supplemented with 0.3 % lignocellulose from days 1 to 21 and 0.6 % from days 22 to 42. Lignocellulose supplementation increased average daily gain in the LC1 group (P < 0.05), and improved feed conversion ratio in the LC2 group before day 28 (P < 0.05). Additionally, lignocellulose increased intestinal length (P < 0.05) and weight (P < 0.05), and improved the intestinal structure (P < 0.05) and antioxidant capacity (P < 0.05). However, the expression of genes associated with tight junctions, immunity, and inflammation was not significantly affected. Overall, dietary lignocellulose promotes growth in meat ducks by improving intestinal morphology and antioxidant capacity without affecting the expression of genes involved in intestinal tight junction and immunity or inflammation. The magnitude of benefits depends on the supplementation level, duration, and the age of birds.

膳食纤维有助于改善肠道形态、屏障完整性和微生物平衡，从而提高动物对养分的利用和生长性能。本试验旨在评价饲粮中添加木质纤维素对肉鸭肠道功能和生长性能的影响。试验选用180只1日龄樱桃谷肉鸭，随机分为对照组（CON）、木质纤维素组1 （LC1）和木质纤维素组2 (LC2)，每组6个重复，每个重复10只鸭。CON组饲喂基础饲粮；LC1组在第1 ~ 42天饲喂在基础饲粮中添加0.6%木质纤维素的试验饲粮，LC2组在第1 ~ 21天和第22 ~ 42天分别饲喂在基础饲粮中添加0.3%和0.6%木质纤维素的试验饲粮。添加木质纤维素提高了LC1组的平均日增重（P < 0.05），提高了LC2组28天前的饲料系数（P < 0.05）。此外，木质纤维素增加了肠道长度（P < 0.05）和重量（P < 0.05），改善了肠道结构（P < 0.05）和抗氧化能力（P < 0.05）。然而，与紧密连接、免疫和炎症相关的基因的表达并未受到显著影响。总之，饲粮中木质纤维素通过改善肠道形态和抗氧化能力促进肉鸭生长，而不影响肠道紧密连接、免疫或炎症相关基因的表达。益处的大小取决于补充水平、持续时间和鸟龄。

{"title":"Dietary supplementation of lignocellulose promotes the growth of Cherry Valley ducks by improving intestinal function","authors":"Xinzhi Geng , Zhenzhen Chen , Jian Wang , Biao Dong , Jing Ge , Minmeng Zhao , Long Liu , Daoqing Gong , Haixia Liu , Tuoyu Geng","doi":"10.1016/j.vas.2025.100558","DOIUrl":"10.1016/j.vas.2025.100558","url":null,"abstract":"<div><div>Dietary fiber contributes to improving intestinal morphology, barrier integrity, and microbial balance, thereby enhancing nutrient utilization and growth performance in animals. This study evaluated the effects of supplementing lignocellulose on intestinal function and growth performance in meat ducks. A total of 180 one-day-old Cherry Valley ducks were randomly assigned to the control group (CON), lignocellulose group 1 (LC1), and lignocellulose group 2 (LC2) (6 replicates each group, 10 ducks each replicate). Ducks in the CON group were fed a basal diet; the LC1 group received the basal diet supplemented with 0.6% lignocellulose from days 1 to 42, while the LC2 group received the basal diet supplemented with 0.3 % lignocellulose from days 1 to 21 and 0.6 % from days 22 to 42. Lignocellulose supplementation increased average daily gain in the LC1 group (<em>P</em> < 0.05), and improved feed conversion ratio in the LC2 group before day 28 (<em>P</em> < 0.05). Additionally, lignocellulose increased intestinal length (<em>P</em> < 0.05) and weight (<em>P</em> < 0.05), and improved the intestinal structure (<em>P</em> < 0.05) and antioxidant capacity (<em>P</em> < 0.05). However, the expression of genes associated with tight junctions, immunity, and inflammation was not significantly affected. Overall, dietary lignocellulose promotes growth in meat ducks by improving intestinal morphology and antioxidant capacity without affecting the expression of genes involved in intestinal tight junction and immunity or inflammation. The magnitude of benefits depends on the supplementation level, duration, and the age of birds.</div></div>","PeriodicalId":37152,"journal":{"name":"Veterinary and Animal Science","volume":"31 ","pages":"Article 100558"},"PeriodicalIF":1.9,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145926585","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

New frontiers in regenerative medicine: Protocol standardization and morphological assessment of leukocyte-platelet rich fibrin in cows, with a longitudinal study on growth factor release dynamics 再生医学的新前沿：牛体内富白细胞血小板纤维蛋白的方案标准化和形态学评估，以及生长因子释放动力学的纵向研究

IF 1.9 Q2 AGRICULTURE, DAIRY & ANIMAL SCIENCE

Veterinary and Animal Science

Pub Date : 2026-03-01 Epub Date: 2025-12-29 DOI: 10.1016/j.vas.2025.100564

Giovanni Della Valle , Maria Chiara Alterisio , Jacopo Guccione , Chiara Caterino , Federica Aragosa , Gianmarco Ferrara , Davide De Biase , Paolo Ciaramella , Gerardo Fatone

Leukocyte-Platelets Rich Fibrin (L-PRF) is a platelet-derived autologous agent. It is a powerful wound-sealing biological scaffold that promotes the healing process through the release of growth factors (GFs) and matrix proteins. To date, the l-PRF in bovines has not yet been investigated and a standardized production protocol has not yet been described. Therefore, the aim of the study was to standardise the l-PRF production protocol and to evaluate macroscopically and histologically the l-PRF membranes obtained and observe the GFs release over time and lactation phases: at 1 h (T0), 4 h (T1), 24 h (T2), and 168 h (T3) after extraction. Eighty cows were divided into four groups of 20 animals, based on their lactation phase. A whole blood sample of 20 ml was taken and divided for l-PRF clot production and haemato-biochemical investigations. The membranes presented the following overall mean characteristics: a length of 34.4 ± 7.3 cm, a width of 12.5 ± 2.6 cm, and a weight of 4.9 ± 0.17 cm. Histological analysis showed a well-defined histoarchitecture consisting of a first layer of erythrocytes, a layer of leukocytes, erythrocytes, and platelets, a layer of leukocytes, a layer of platelets, and finally a layer of slightly eosinophilic fibrin. The GFs significantly increased with upward trends in all phases of lactation and at each time control, overlapping the behavior of GF in other species. Based on the encouraging findings of this study, further clinical trials for applicative potential of the l-PRF membrane in bovine medicine should be assessed.

富白细胞血小板纤维蛋白（L-PRF）是一种血小板来源的自体药物。它是一种强大的伤口密封生物支架，通过释放生长因子（GFs）和基质蛋白来促进愈合过程。迄今为止，尚未对牛的l-PRF进行调查，也尚未描述标准化生产方案。因此，本研究的目的是标准化l-PRF的生产方案，从宏观和组织学上评价所获得的l-PRF膜，并观察提取后1小时（T0）、4小时（T1）、24小时（T2）和168小时（T3）随时间和哺乳期的GFs释放情况。80头奶牛根据哺乳期分为4组，每组20头。取全血20 ml，分离用于l-PRF凝块产生和血液生化检查。膜的平均长度为34.4±7.3 cm，宽度为12.5±2.6 cm，重量为4.9±0.17 cm。组织学分析显示一个明确的组织结构，包括第一层红细胞，一层白细胞，红细胞和血小板，一层白细胞，一层血小板，最后一层微嗜酸性纤维蛋白。在哺乳期各阶段和各时间对照组中，GF均呈上升趋势，显著增加，与其他物种GF的行为重叠。基于这一令人鼓舞的研究结果，应该对l-PRF膜在牛医学中的应用潜力进行进一步的临床试验。

{"title":"New frontiers in regenerative medicine: Protocol standardization and morphological assessment of leukocyte-platelet rich fibrin in cows, with a longitudinal study on growth factor release dynamics","authors":"Giovanni Della Valle , Maria Chiara Alterisio , Jacopo Guccione , Chiara Caterino , Federica Aragosa , Gianmarco Ferrara , Davide De Biase , Paolo Ciaramella , Gerardo Fatone","doi":"10.1016/j.vas.2025.100564","DOIUrl":"10.1016/j.vas.2025.100564","url":null,"abstract":"<div><div>Leukocyte-Platelets Rich Fibrin (L-PRF) is a platelet-derived autologous agent. It is a powerful wound-sealing biological scaffold that promotes the healing process through the release of growth factors (GFs) and matrix proteins. To date, the <span>l</span>-PRF in bovines has not yet been investigated and a standardized production protocol has not yet been described. Therefore, the aim of the study was to standardise the <span>l</span>-PRF production protocol and to evaluate macroscopically and histologically the <span>l</span>-PRF membranes obtained and observe the GFs release over time and lactation phases: at 1 h (T0), 4 h (T1), 24 h (T2), and 168 h (T3) after extraction. Eighty cows were divided into four groups of 20 animals, based on their lactation phase. A whole blood sample of 20 ml was taken and divided for <span>l</span>-PRF clot production and haemato-biochemical investigations. The membranes presented the following overall mean characteristics: a length of 34.4 ± 7.3 cm, a width of 12.5 ± 2.6 cm, and a weight of 4.9 ± 0.17 cm. Histological analysis showed a well-defined histoarchitecture consisting of a first layer of erythrocytes, a layer of leukocytes, erythrocytes, and platelets, a layer of leukocytes, a layer of platelets, and finally a layer of slightly eosinophilic fibrin. The GFs significantly increased with upward trends in all phases of lactation and at each time control, overlapping the behavior of GF in other species. Based on the encouraging findings of this study, further clinical trials for applicative potential of the <span>l</span>-PRF membrane in bovine medicine should be assessed.</div></div>","PeriodicalId":37152,"journal":{"name":"Veterinary and Animal Science","volume":"31 ","pages":"Article 100564"},"PeriodicalIF":1.9,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145926586","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Natural infection with Trypanosoma cruzi in a litter of dogs (Canis lupus familiaris) from Floridablanca, Colombia, suggests congenital transmission and a potential zoonotic risk 哥伦比亚佛罗里达布兰卡一窝狗自然感染克氏锥虫（犬狼疮）表明存在先天性传播和潜在人畜共患风险

IF 1.9 Q2 AGRICULTURE, DAIRY & ANIMAL SCIENCE

Veterinary and Animal Science

Pub Date : 2026-03-01 Epub Date: 2025-12-22 DOI: 10.1016/j.vas.2025.100562

Jeiczon Jaimes-Dueñez , María Victoria Esteban-Mendoza , Ian Sebastián Murcia-Cueto , Luz Zoraya Beatriz Duarte-Rodríguez , Genny Liliana Uribe Gómez , Harley Díaz Rincón , Javier Alonso Herrera Cuadros , Omar Cantillo-Barraza

Chagas disease (CD) remains a major public health challenge in endemic regions of Colombia, where both domestic and sylvatic host sustain transmission. This study reports the first natural infection with Trypanosoma cruzi in a litter of dogs from Floridablanca, in the Metropolitan Area of Bucaramanga (MAB), Santander, most likely associated with congenital transmission. Eight puppies and their dam were clinically evaluated, and blood samples were analyzed through hematology, quantitative real-time PCR (qPCR), multiplex PCR for discrete typing units (DTUs) identification, and hemoculture. Serological surveillance was also performed in the owners’ nuclear family. All puppies and the dam tested positive for T. cruzi DNA by qPCR, with high parasitemia levels (751.8–977.3 parasite equivalents/mL in puppies and 526.2 parasite equivalents/mL in the dam), corresponding to DTU I (TcI). Hematological findings in puppies indicated regenerative anemia, thrombocytopenia, and lymphocytosis. Despite supportive treatment, seven puppies died (mortality rate = 87.5 %), and one was euthanized after three months of disease progression. Postmortem examination revealed multisystemic lesions, including interstitial pneumonia, necrotizing hepatitis, myocardial lymphocytic infiltrates with amastigote cysts, and meningoencephalitis, consistent with acute trypanosomiasis. Hemoculture was positive in this case. None of the family members tested positive for T. cruzi antibodies. To our knowledge, this is the first description of natural infection with T. cruzi DTU I (TcI) in a litter of dogs from Colombia. The marked parasitemia observed, together with hemoculture positivity, highlights the potential of these hosts to infect triatomine vectors and increase the risk of CD transmission in endemic areas.

恰加斯病（CD）仍然是哥伦比亚流行地区的一个主要公共卫生挑战，在这些地区，家庭宿主和森林宿主都持续传播。本研究报告了在桑坦德布卡拉曼加大都会区（MAB）佛罗里达布兰卡的一窝狗中首次自然感染克氏锥虫，很可能与先天性传播有关。对8只幼犬及其母鼠进行临床评估，并对其血液样本进行血液学、定量实时PCR （qPCR）、多重PCR分离分型单元（DTUs）鉴定和血液培养分析。对业主核心家庭进行血清学监测。所有幼犬和母鼠均qPCR检测克氏体DNA阳性，较高的寄生水平（幼犬为751.8 ~ 977.3个寄生当量/mL，母鼠为526.2个寄生当量/mL），符合DTU I （TcI）。小狗的血液学结果显示再生性贫血、血小板减少症和淋巴细胞增多症。尽管进行了支持性治疗，但仍有7只小狗死亡（死亡率= 87.5%），其中一只在疾病进展三个月后被安乐死。尸检显示多系统病变，包括间质性肺炎、坏死性肝炎、心肌淋巴细胞浸润伴无鞭虫囊肿和脑膜脑炎，与急性锥虫病一致。本例血液培养呈阳性。没有家庭成员的克氏体抗体检测呈阳性。据我们所知，这是首次在哥伦比亚一窝狗中发现克氏t型虫DTU I （TcI）自然感染。所观察到的明显寄生虫病，加上血液培养阳性，突出了这些宿主感染锥蝽病媒的潜力，并增加了流行地区传播乳糜泻的风险。

{"title":"Natural infection with Trypanosoma cruzi in a litter of dogs (Canis lupus familiaris) from Floridablanca, Colombia, suggests congenital transmission and a potential zoonotic risk","authors":"Jeiczon Jaimes-Dueñez , María Victoria Esteban-Mendoza , Ian Sebastián Murcia-Cueto , Luz Zoraya Beatriz Duarte-Rodríguez , Genny Liliana Uribe Gómez , Harley Díaz Rincón , Javier Alonso Herrera Cuadros , Omar Cantillo-Barraza","doi":"10.1016/j.vas.2025.100562","DOIUrl":"10.1016/j.vas.2025.100562","url":null,"abstract":"<div><div>Chagas disease (CD) remains a major public health challenge in endemic regions of Colombia, where both domestic and sylvatic host sustain transmission. This study reports the first natural infection with <em>Trypanosoma cruzi</em> in a litter of dogs from Floridablanca, in the Metropolitan Area of Bucaramanga (MAB), Santander, most likely associated with congenital transmission. Eight puppies and their dam were clinically evaluated, and blood samples were analyzed through hematology, quantitative real-time PCR (qPCR), multiplex PCR for discrete typing units (DTUs) identification, and hemoculture. Serological surveillance was also performed in the owners’ nuclear family. All puppies and the dam tested positive for <em>T. cruzi</em> DNA by qPCR, with high parasitemia levels (751.8–977.3 parasite equivalents/mL in puppies and 526.2 parasite equivalents/mL in the dam), corresponding to DTU I (TcI). Hematological findings in puppies indicated regenerative anemia, thrombocytopenia, and lymphocytosis. Despite supportive treatment, seven puppies died (mortality rate = 87.5 %), and one was euthanized after three months of disease progression. Postmortem examination revealed multisystemic lesions, including interstitial pneumonia, necrotizing hepatitis, myocardial lymphocytic infiltrates with amastigote cysts, and meningoencephalitis, consistent with acute trypanosomiasis. Hemoculture was positive in this case. None of the family members tested positive for <em>T. cruzi</em> antibodies. To our knowledge, this is the first description of natural infection with <em>T. cruzi</em> DTU I (TcI) in a litter of dogs from Colombia. The marked parasitemia observed, together with hemoculture positivity, highlights the potential of these hosts to infect triatomine vectors and increase the risk of CD transmission in endemic areas.</div></div>","PeriodicalId":37152,"journal":{"name":"Veterinary and Animal Science","volume":"31 ","pages":"Article 100562"},"PeriodicalIF":1.9,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145977892","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effects of an optimized dairy calf-rearing protocol on performance and health in the subsequent fattening period on Swiss veal farms 优化的奶牛饲养方案对瑞士小牛肉农场后期育肥期生产性能和健康的影响

IF 1.9 Q2 AGRICULTURE, DAIRY & ANIMAL SCIENCE

Veterinary and Animal Science

Pub Date : 2026-03-01 Epub Date: 2026-01-30 DOI: 10.1016/j.vas.2026.100590

Julia Rell , Michael Walkenhorst , Mirjam Holinger , Corinne Bähler , Jens Becker , Martin Kaske

We conducted a prospective field study to characterize the effects of an optimized rearing protocol of calves on dairy-farms on the subsequent performance, health and antimicrobial use (AMU) at the veal-farm. On 19 dairy-farms a total of 55 matched pairs of calves (same gender and breeding type, born within the same period and farm) were randomly assigned to the treatment group P (“preconditioned”) or C (“control”) during five two-week enrollment periods. The P-calves were fed milk ad libitum, received parenteral selenium and iron supplementation, wore calf jackets at low temperatures, and were vaccinated intranasally against bovine respiratory disease. The C-calves were fed restrictively and received no additional measures. At an average of 30 days of life, calves were transported directly to one of two veal-farms. Body weight and health score were determined between days 2 and 5 of life, at transport and in weeks 4 and 12 after arrival on the veal farm. All applications of antimicrobials were recorded. Mixed-effect models were applied for statistical analyses. While born with similar body weights, daily weight gain of the calves differed between groups on the dairy-farms (P: 1.10[0.96–1.25] vs. C: 0.70[0.55–0.84]kg/day; p < 0.001) but not on the veal-farms (P: 1.34[1.24–1.44] vs. C: 1.29[1.19–1.39]kg/day). A similar carcass weight was reached in shorter time for P-calves compared to C-calves (104[99–110] vs. 115[109–120] days on the veal-farm; p = 0.04). Neither clinical health nor AMU differed between the groups. Thus, while preconditioning was effective regarding performance parameters, a significant advantage in respect of health status and AMU could not be demonstrated.

我们进行了一项前瞻性的实地研究，以表征奶牛农场小牛的优化饲养方案对小牛农场随后的生产性能、健康和抗菌药物使用（AMU）的影响。在19个奶牛场中，共有55对匹配的小牛（相同性别和繁殖类型，在同一时期和同一农场出生）被随机分配到治疗组P（“预处理”）或C（“对照”），为期5周。这些p型犊牛被随意喂养牛奶，经肠外补充硒和铁，在低温下穿着犊牛夹克，并鼻内接种牛呼吸道疾病疫苗。c型犊牛限饲，不采取任何额外措施。在平均30天的生命中，小牛被直接运送到两个小牛肉农场中的一个。在出生后的第2天至第5天、运输时以及抵达小牛肉农场后的第4周和第12周测定体重和健康评分。记录所有抗菌素的应用情况。采用混合效应模型进行统计分析。犊牛出生时体重相似，但乳牛场各组犊牛日增重差异较大（P: 1.10[0.96-1.25] vs. C: 0.70[0.55-0.84]kg/d; P < 0.001），而小牛肉场各组犊牛日增重差异较小（P: 1.34[1.24-1.44] vs. C: 1.29[1.19-1.39]kg/d）。与c型犊牛相比，p型犊牛在更短的时间内达到相似的胴体重量（小牛养殖场104[99-110]天和115[109-120]天，p = 0.04）。两组之间的临床健康和AMU都没有差异。因此，虽然预处理在性能参数方面是有效的，但在健康状态和AMU方面却没有明显的优势。

{"title":"Effects of an optimized dairy calf-rearing protocol on performance and health in the subsequent fattening period on Swiss veal farms","authors":"Julia Rell , Michael Walkenhorst , Mirjam Holinger , Corinne Bähler , Jens Becker , Martin Kaske","doi":"10.1016/j.vas.2026.100590","DOIUrl":"10.1016/j.vas.2026.100590","url":null,"abstract":"<div><div>We conducted a prospective field study to characterize the effects of an optimized rearing protocol of calves on dairy-farms on the subsequent performance, health and antimicrobial use (AMU) at the veal-farm. On 19 dairy-farms a total of 55 matched pairs of calves (same gender and breeding type, born within the same period and farm) were randomly assigned to the treatment group P (“preconditioned”) or C (“control”) during five two-week enrollment periods. The P-calves were fed milk ad libitum, received parenteral selenium and iron supplementation, wore calf jackets at low temperatures, and were vaccinated intranasally against bovine respiratory disease. The C-calves were fed restrictively and received no additional measures. At an average of 30 days of life, calves were transported directly to one of two veal-farms. Body weight and health score were determined between days 2 and 5 of life, at transport and in weeks 4 and 12 after arrival on the veal farm. All applications of antimicrobials were recorded. Mixed-effect models were applied for statistical analyses. While born with similar body weights, daily weight gain of the calves differed between groups on the dairy-farms (P: 1.10[0.96–1.25] vs. C: 0.70[0.55–0.84]kg/day; <em>p</em> < 0.001) but not on the veal-farms (P: 1.34[1.24–1.44] vs. C: 1.29[1.19–1.39]kg/day). A similar carcass weight was reached in shorter time for P-calves compared to C-calves (104[99–110] vs. 115[109–120] days on the veal-farm; <em>p</em> = 0.04). Neither clinical health nor AMU differed between the groups. Thus, while preconditioning was effective regarding performance parameters, a significant advantage in respect of health status and AMU could not be demonstrated.</div></div>","PeriodicalId":37152,"journal":{"name":"Veterinary and Animal Science","volume":"31 ","pages":"Article 100590"},"PeriodicalIF":1.9,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147419782","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0