The use of stabilizing technologies has significantly expanded in recent years specially when sites are frequently construction in poor land locations. This study suggests using nano-silica to improve clayey soil's functionality. A range of nano-silica concentrations (0, 0.1, 0.2, 0.4, and 0.8) were used. Laboratory testing was used to identify Atterber's limitations, the optimum moisture content (OMC), the maximum dry density (MDD), and microstructural examination. According to the study, treating soil with 0.4-0.6% nano-silica yields the best results. According to the result, the liquid limit (LL) and plastic limit (PL) are reached maximum at 0.6% nano-silica, while the plasticity index is at its lowest point. The results showed that incorporating nano-silica into clay samples will lower the maximum.
{"title":"Enhancement consistency and compaction characteristics of clayey soil using nano silica material","authors":"Najwa Wasif Jassim, Hanan Adnan Hassan, Hadeel Ammar Mohammed, Mohammed Yousif Fattah","doi":"10.21533/pen.v10i6.3367","DOIUrl":"https://doi.org/10.21533/pen.v10i6.3367","url":null,"abstract":"The use of stabilizing technologies has significantly expanded in recent years specially when sites are frequently construction in poor land locations. This study suggests using nano-silica to improve clayey soil's functionality. A range of nano-silica concentrations (0, 0.1, 0.2, 0.4, and 0.8) were used. Laboratory testing was used to identify Atterber's limitations, the optimum moisture content (OMC), the maximum dry density (MDD), and microstructural examination. According to the study, treating soil with 0.4-0.6% nano-silica yields the best results. According to the result, the liquid limit (LL) and plastic limit (PL) are reached maximum at 0.6% nano-silica, while the plasticity index is at its lowest point. The results showed that incorporating nano-silica into clay samples will lower the maximum.","PeriodicalId":37519,"journal":{"name":"Periodicals of Engineering and Natural Sciences","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-01-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45350143","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Flexible pavement is usually designed based on certain axle load limits and climatic conditions. The Iraqi code has specified certain load limits per each axle type that should not be exceeded. However, many trucks violate these limits by carrying additional weights to decrease the transportation cost. These overweight trucks cause severe deterioration to the pavement and thus reduce its life. The Iraqi authorities generally charges the violating trucks a penalty based on their weights. This penalty could be very small compared to the damage occurring to the pavement based on these over weights. Also, some trucks may carry huge weights that the pavement may not support, so unloading such trucks could be a suitable solution rather than paying few amounts of money and deteriorating the pavement. The study aims at studying the effect of axle load increase, and the variation in pavement moduli, on the overall pavement life. It also aims to estimate the overweight truck limits that could be penalized or unloaded. The research uses the ABAQUS software conditions to estimate the tensile strains occurring under the asphalt concrete (AC) layer and the compressive strains above the subgrade surface. These computed strains are incorporated in the fatigue cracking and rutting models to estimate the pavement life for different axle weights. Results showed that violating trucks should be unloaded when their weights exceed certain limits.
{"title":"Deformation behavior of flexible pavements by finite element simulation","authors":"Duaa S. Alwan, H. Joni, M. Hilal","doi":"10.21533/pen.v10i6.3392","DOIUrl":"https://doi.org/10.21533/pen.v10i6.3392","url":null,"abstract":"Flexible pavement is usually designed based on certain axle load limits and climatic conditions. The Iraqi code has specified certain load limits per each axle type that should not be exceeded. However, many trucks violate these limits by carrying additional weights to decrease the transportation cost. These overweight trucks cause severe deterioration to the pavement and thus reduce its life. The Iraqi authorities generally charges the violating trucks a penalty based on their weights. This penalty could be very small compared to the damage occurring to the pavement based on these over weights. Also, some trucks may carry huge weights that the pavement may not support, so unloading such trucks could be a suitable solution rather than paying few amounts of money and deteriorating the pavement. The study aims at studying the effect of axle load increase, and the variation in pavement moduli, on the overall pavement life. It also aims to estimate the overweight truck limits that could be penalized or unloaded. The research uses the ABAQUS software conditions to estimate the tensile strains occurring under the asphalt concrete (AC) layer and the compressive strains above the subgrade surface. These computed strains are incorporated in the fatigue cracking and rutting models to estimate the pavement life for different axle weights. Results showed that violating trucks should be unloaded when their weights exceed certain limits.","PeriodicalId":37519,"journal":{"name":"Periodicals of Engineering and Natural Sciences","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41712413","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
The article presents the development of a mathematical model of video monitoring based on a self-organizing network of unmanned aerial vehicles. The necessity of developing models and algorithms for providing geoecological monitoring using a wireless self-organizing network based on unmanned aerial vehicles is shown. Models are presented that allow calculating the speed of information transfer in the network and reducing the number of failures in the process of transmitting video data. With the help of models, it is possible to substantiate the power of network transmitting devices, at which the losses of transmitted packets are significantly reduced. The practical use of the model contributes to the achievement of the required quality of video surveillance in a wireless self-organizing network of unmanned aerial vehicles in the process of geoecological monitoring.
{"title":"Development of a mathematical model of video monitoring based on a self-organizing network of unmanned aerial vehicles","authors":"J. Q. Jameel, ,. E. A. Tareq Nasser Mahdi","doi":"10.21533/pen.v10i6.3381","DOIUrl":"https://doi.org/10.21533/pen.v10i6.3381","url":null,"abstract":"The article presents the development of a mathematical model of video monitoring based on a self-organizing network of unmanned aerial vehicles. The necessity of developing models and algorithms for providing geoecological monitoring using a wireless self-organizing network based on unmanned aerial vehicles is shown. Models are presented that allow calculating the speed of information transfer in the network and reducing the number of failures in the process of transmitting video data. With the help of models, it is possible to substantiate the power of network transmitting devices, at which the losses of transmitted packets are significantly reduced. The practical use of the model contributes to the achievement of the required quality of video surveillance in a wireless self-organizing network of unmanned aerial vehicles in the process of geoecological monitoring.","PeriodicalId":37519,"journal":{"name":"Periodicals of Engineering and Natural Sciences","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44015313","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Repair of cracked pipeline under internal pressure has been investigated in the present study. To this aim, an experimental test has been done on the cracked pipeline to find failure pressure. A longitudinal crack that cut 65% thickness of pipe has been applied on the external surface. Carbon fiber reinforced polymer has been used to repair the system. Additionally, a finite element model has been developed to estimate failure pressure of the unrepaired pipes. The results show that the failure pressure in unrepaired pipes is identical to the failure pressure predicted by the standards for corroded pipes, however, the failure pressure of repaired system is lower than the predicted results of standard for corroded pipelines.
{"title":"Experimental study on repair of cracked pipe under internal pressure","authors":"Ali Abduljabbar, Haider Khazal, A. F. Hassan","doi":"10.21533/pen.v10i6.3369","DOIUrl":"https://doi.org/10.21533/pen.v10i6.3369","url":null,"abstract":"Repair of cracked pipeline under internal pressure has been investigated in the present study. To this aim, an experimental test has been done on the cracked pipeline to find failure pressure. A longitudinal crack that cut 65% thickness of pipe has been applied on the external surface. Carbon fiber reinforced polymer has been used to repair the system. Additionally, a finite element model has been developed to estimate failure pressure of the unrepaired pipes. The results show that the failure pressure in unrepaired pipes is identical to the failure pressure predicted by the standards for corroded pipes, however, the failure pressure of repaired system is lower than the predicted results of standard for corroded pipelines.","PeriodicalId":37519,"journal":{"name":"Periodicals of Engineering and Natural Sciences","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48329558","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
The rapid enhancement in the fields of the computers that leads to rapid breaking for ciphering algorithms and for these reasons most of ciphering algorithm tried to used multidigit for ciphering texts or images. Using multidigit will increase the safety of information and protected it from supercomputer from breaking the ciphering algorithms. The current information systems employ operations on finite fields of various structures (for example, cryptographic systems). In this instance, it's common to have to deal with enormous numbers (128 bits or more). The proposed operation of discovering the remainder of the division of multidigit numbers will considerably improve the speed of such systems if implemented.
{"title":"Study of effective calculation operation implementation remaining multi-bit numbers division on FPGA","authors":"A. Al-Hilali, Laith F. Jumaa, Ibrahim A. Amory","doi":"10.21533/pen.v10i6.3382","DOIUrl":"https://doi.org/10.21533/pen.v10i6.3382","url":null,"abstract":"The rapid enhancement in the fields of the computers that leads to rapid breaking for ciphering algorithms and for these reasons most of ciphering algorithm tried to used multidigit for ciphering texts or images. Using multidigit will increase the safety of information and protected it from supercomputer from breaking the ciphering algorithms. The current information systems employ operations on finite fields of various structures (for example, cryptographic systems). In this instance, it's common to have to deal with enormous numbers (128 bits or more). The proposed operation of discovering the remainder of the division of multidigit numbers will considerably improve the speed of such systems if implemented.","PeriodicalId":37519,"journal":{"name":"Periodicals of Engineering and Natural Sciences","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48426678","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Wavelet reduction is one of the most widely used methods for removing noise from the signal, primarily financial and banking data, and building a non-parametric regression model that enables us to study the phenomenon accurately. The appropriate choice of the wavelet mother, which results in concentrating the bulk of the signal strength on a few wavelet coefficients, is one of the most determining factors in noise removal and obtaining accurate regression function estimates. Given the importance of studying the price index in Iraq, many mother functions within the wavelet transformation have been studied. To determine which of them is more suitable for such a type of data, which gives accurate estimates of the relationship between trading volume as an independent variable and the Iraq market index as a dependent variable, the best or most appropriate functions were determined through the estimates that have less (MSE). It became clear that the best or relevant parts are (Coif1, Coif5, and rbio1.3). The study was applied to real data represented by the trading volume and price index data for the Iraqi market for the period from (2008) to April (2022). It became clear that the trading volume significantly affects the price index, but other variables must be studied .
{"title":"Estimation of nonparametric regression function using shrinkage wavelet and different mother functions","authors":"Saad Kadem Hamza, S. Ali","doi":"10.21533/pen.v10i6.3386","DOIUrl":"https://doi.org/10.21533/pen.v10i6.3386","url":null,"abstract":"Wavelet reduction is one of the most widely used methods for removing noise from the signal, primarily financial and banking data, and building a non-parametric regression model that enables us to study the phenomenon accurately. The appropriate choice of the wavelet mother, which results in concentrating the bulk of the signal strength on a few wavelet coefficients, is one of the most determining factors in noise removal and obtaining accurate regression function estimates. Given the importance of studying the price index in Iraq, many mother functions within the wavelet transformation have been studied. To determine which of them is more suitable for such a type of data, which gives accurate estimates of the relationship between trading volume as an independent variable and the Iraq market index as a dependent variable, the best or most appropriate functions were determined through the estimates that have less (MSE). It became clear that the best or relevant parts are (Coif1, Coif5, and rbio1.3). The study was applied to real data represented by the trading volume and price index data for the Iraqi market for the period from (2008) to April (2022). It became clear that the trading volume significantly affects the price index, but other variables must be studied .","PeriodicalId":37519,"journal":{"name":"Periodicals of Engineering and Natural Sciences","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48535485","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
This research is carried out to investigate and assess the critical role of soil properties and their content in influencing the soil liquefaction phenomenon that takes place when an earthquake happens. A case study is considered in this work, representing the Karakaya Dam. Two research approaches were implemented to achieve the research goal, including extrapolation in Excel and numerical optimization using linear regression. The outputs revealed that the annual temperature in the future of zones around the Karakaya Dam, including Azami, Ortalama, and Asgari, would witness a moderate increase of about 3 to 6 degrees Celsius in the next decade. Moreover, the research confirmed that the Euphrates River discharge rate at the Karakaya Dam would witness a significant increase from (100-350 m 3 ) to (2,590-2,640 m 3 ) in 2029, explaining that temperature and discharge rate may influence the liquefaction. Meanwhile, the research outputs indicated that soil temperature under the Karakaya Dam and chemical elements would not vary significantly in the next decade. Notwithstanding, the pH number will change widely from 4.14 to 9.74 in 2029. Besides, the most significant chemical molecule concentration in the soil under the Karakaya Dam is the phosphite anion, corresponding to a minimum and maximum concentration of 1 and 2 μg/m 2 , respectively.
{"title":"Investigating the potential of liquefaction issue of some Turkish dams during earthquakes","authors":"L. Mahmood, S. Naimi","doi":"10.21533/pen.v10i6.3375","DOIUrl":"https://doi.org/10.21533/pen.v10i6.3375","url":null,"abstract":"This research is carried out to investigate and assess the critical role of soil properties and their content in influencing the soil liquefaction phenomenon that takes place when an earthquake happens. A case study is considered in this work, representing the Karakaya Dam. Two research approaches were implemented to achieve the research goal, including extrapolation in Excel and numerical optimization using linear regression. The outputs revealed that the annual temperature in the future of zones around the Karakaya Dam, including Azami, Ortalama, and Asgari, would witness a moderate increase of about 3 to 6 degrees Celsius in the next decade. Moreover, the research confirmed that the Euphrates River discharge rate at the Karakaya Dam would witness a significant increase from (100-350 m 3 ) to (2,590-2,640 m 3 ) in 2029, explaining that temperature and discharge rate may influence the liquefaction. Meanwhile, the research outputs indicated that soil temperature under the Karakaya Dam and chemical elements would not vary significantly in the next decade. Notwithstanding, the pH number will change widely from 4.14 to 9.74 in 2029. Besides, the most significant chemical molecule concentration in the soil under the Karakaya Dam is the phosphite anion, corresponding to a minimum and maximum concentration of 1 and 2 μg/m 2 , respectively.","PeriodicalId":37519,"journal":{"name":"Periodicals of Engineering and Natural Sciences","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48996756","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
The current study is designed to investigate the histological and immunohistochemical characteristics of the thyroid gland in adult male Sciurus anamalus . This study found that the thyroid gland of the Caucasian squirrel is located in the neck area, below the larynx, on both sides of the trachea. It has two lobes (right and left) with cylindrical shape. The histological studies revealed that the thyroid gland is surrounded by a capsule which consists of connective tissue and forming of two layers which are outer layer and inner layer, and a layer of adipose tissue appears overlapping the outer layer. The inner tissue of the gland consists of follicles with different shapes and sizes, and is lined with simple cuboidal epithelial tissue (follicular cells). Sometimes it appears in the form of high or low cuboidal epithelial tissue, in dependent on the functional activity state of the gland, and the follicles cavities are filled with colloid. In addition, there are two kinds of cells spread in the gland tissue, follicular cells and parafollicular cells, and they are fewer in number and larger in size than follicular cells and appear either singly or in the form of clusters and occupy two sites between the follicles or within the follicle. Immunologically, the results observed an immune response to thyroglobulin antibodies to detect the protein secreted by the follicular cells, which in turn secreted it to the colloid. Moreover, the immune response appeared clear in the cell membrane of the follicular cells and the colloid through the appearance of the brown color which is specific to the immune reaction.
{"title":"Histological and immunohistochemical study of thyroid gland in Caucasian squirrel (Sciurus anamalus) (Gmelin, 1778) by using marker (Anti-Thyroglobulin, Code IR5090)","authors":"N. M. J. Hammodi, Rana Alaa Al Aamery","doi":"10.21533/pen.v10i6.3383","DOIUrl":"https://doi.org/10.21533/pen.v10i6.3383","url":null,"abstract":"The current study is designed to investigate the histological and immunohistochemical characteristics of the thyroid gland in adult male Sciurus anamalus . This study found that the thyroid gland of the Caucasian squirrel is located in the neck area, below the larynx, on both sides of the trachea. It has two lobes (right and left) with cylindrical shape. The histological studies revealed that the thyroid gland is surrounded by a capsule which consists of connective tissue and forming of two layers which are outer layer and inner layer, and a layer of adipose tissue appears overlapping the outer layer. The inner tissue of the gland consists of follicles with different shapes and sizes, and is lined with simple cuboidal epithelial tissue (follicular cells). Sometimes it appears in the form of high or low cuboidal epithelial tissue, in dependent on the functional activity state of the gland, and the follicles cavities are filled with colloid. In addition, there are two kinds of cells spread in the gland tissue, follicular cells and parafollicular cells, and they are fewer in number and larger in size than follicular cells and appear either singly or in the form of clusters and occupy two sites between the follicles or within the follicle. Immunologically, the results observed an immune response to thyroglobulin antibodies to detect the protein secreted by the follicular cells, which in turn secreted it to the colloid. Moreover, the immune response appeared clear in the cell membrane of the follicular cells and the colloid through the appearance of the brown color which is specific to the immune reaction.","PeriodicalId":37519,"journal":{"name":"Periodicals of Engineering and Natural Sciences","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49011548","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Antibacterial resistant bacteria cause a big concern to poultry and to public health in general because some bacterial poultry pathogens can infect humans or transfer their resistance ability to human pathogens. Nontherapeutic use of antibacterial in poultry, especially as growth promoters to increase feed conversion efficiency is thought to be one of the main causes of resistance. The study included pulmonary swab samples collected during necropsy from 120 poultry farms showing respiratory symptoms with mortality. The disc diffusion method for antibiotic sensitivity testing was performed and antibiotic disks for 21 antibiotics were used. The results showed that three antibacterial were sensitive to more than 50% of the isolates. The first is doxycycline and 69.9% of the isolates were sensitive. The second is Cefalexin with 60.5% sensitive isolates and third is Chloramphenicol with 55.2% sensitive isolates. In the rest of antibacterial, less than 50% were sensitive. Five isolates were found resistant to all antibacterial. Moreover, three samples were found to be negative with no bacterial growth. The present study concluded that 50% of the aerobic bacteria isolated from poultry lungs are resistant to 85% of the 21 antibiotics tested in the study.
{"title":"Antimicrobial resistance in aerobic bacterial isolates from broiler lungs","authors":"Z. Ibrahim, Nawal S. Jaafer, Hadeel M. Fayyadh","doi":"10.21533/pen.v10i6.3380","DOIUrl":"https://doi.org/10.21533/pen.v10i6.3380","url":null,"abstract":"Antibacterial resistant bacteria cause a big concern to poultry and to public health in general because some bacterial poultry pathogens can infect humans or transfer their resistance ability to human pathogens. Nontherapeutic use of antibacterial in poultry, especially as growth promoters to increase feed conversion efficiency is thought to be one of the main causes of resistance. The study included pulmonary swab samples collected during necropsy from 120 poultry farms showing respiratory symptoms with mortality. The disc diffusion method for antibiotic sensitivity testing was performed and antibiotic disks for 21 antibiotics were used. The results showed that three antibacterial were sensitive to more than 50% of the isolates. The first is doxycycline and 69.9% of the isolates were sensitive. The second is Cefalexin with 60.5% sensitive isolates and third is Chloramphenicol with 55.2% sensitive isolates. In the rest of antibacterial, less than 50% were sensitive. Five isolates were found resistant to all antibacterial. Moreover, three samples were found to be negative with no bacterial growth. The present study concluded that 50% of the aerobic bacteria isolated from poultry lungs are resistant to 85% of the 21 antibiotics tested in the study.","PeriodicalId":37519,"journal":{"name":"Periodicals of Engineering and Natural Sciences","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47425389","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-12-26DOI: 10.28978/nesciences.1224554
M. Doğan
In this work, axillary shoot regenerations were investigated from nodal explants of Hygrophila polysperma (Roxb.) T. Anderson in MS medium (Murashige and Skoog) including different combinations of Gibberellic Acid (GA3) and Naphthalene Acetic Acid (NAA). Nodal explants of H. polysperma were used in propagation studies. The 100% regeneration frequency was determined in MS plus 0.25 and 0.50 mg/L GA3 + 0.15 mg/L NAA. When the shoot numbers were examined, the best nutrient medium was determined as 0.25 mg/L GA3 + 0.15 mg/L NAA (14.72 shoots/explant). The best result in shoot length was reached in MS plus 0.50 mg/L GA3 + 0.15 mg/L NAA (1.91 cm). Then the longest shoot was determined as 1.79 cm in nutrient media plus 0.25 mg/L GA3 + 0.15 mg/L NAA. Longer shoots were obtained in GA3+NAA nutrient media according to control. When the results were examined, it was determined that the number and length of shoots decreased as the amount of GA3+NAA used in the culture medium increased. Regenerated shoots were rooted in MS media including 0.25 mg/L Indole-3-Acetic Acid and successfully acclimatized to aquarium conditions. As a result, multiple and rapid productions of H. polysperma under tissue culture conditions were achieved.
{"title":"Impact of Gibberellic Acid and Naphthalene Acetic Acid on Axillary Shoot Multiplication in Hygrophila polysperma (Roxb.) T. Anderson","authors":"M. Doğan","doi":"10.28978/nesciences.1224554","DOIUrl":"https://doi.org/10.28978/nesciences.1224554","url":null,"abstract":"In this work, axillary shoot regenerations were investigated from nodal explants of Hygrophila polysperma (Roxb.) T. Anderson in MS medium (Murashige and Skoog) including different combinations of Gibberellic Acid (GA3) and Naphthalene Acetic Acid (NAA). Nodal explants of H. polysperma were used in propagation studies. The 100% regeneration frequency was determined in MS plus 0.25 and 0.50 mg/L GA3 + 0.15 mg/L NAA. When the shoot numbers were examined, the best nutrient medium was determined as 0.25 mg/L GA3 + 0.15 mg/L NAA (14.72 shoots/explant). The best result in shoot length was reached in MS plus 0.50 mg/L GA3 + 0.15 mg/L NAA (1.91 cm). Then the longest shoot was determined as 1.79 cm in nutrient media plus 0.25 mg/L GA3 + 0.15 mg/L NAA. Longer shoots were obtained in GA3+NAA nutrient media according to control. When the results were examined, it was determined that the number and length of shoots decreased as the amount of GA3+NAA used in the culture medium increased. Regenerated shoots were rooted in MS media including 0.25 mg/L Indole-3-Acetic Acid and successfully acclimatized to aquarium conditions. As a result, multiple and rapid productions of H. polysperma under tissue culture conditions were achieved.","PeriodicalId":37519,"journal":{"name":"Periodicals of Engineering and Natural Sciences","volume":"14 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-12-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"73360428","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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