Pub Date : 2021-10-15DOI: 10.22073/PJA.V10I4.68717
Asadollah Hosseini Chegeni, F. Faghihi, M. Sharifdini, Z. Telmadarraiy
Thirty tick samples were collected from a Caspian red deer in forested areas of Talesh County, Lisar protected area, Gilan province, northern Iran. Tick samples were examined according to morphological characters. In order to specific identification, a fragment (ca. 800-bp) of internal transcribed spacer 2 (ITS2) and a 340-bp fragment of mitochondrial large subunit ribosomal RNA (16S rRNA) gene were amplified by PCR. Nucleotide sequences were analyzed with BLASTn in GenBank. Adult tick samples were identified as female of the genus (subgenus): Rhipicephalus (Boophilus). The results of BLASTn showed that the female ticks belonged to the species Rhipicephalus (Boophilus) annulatus. Our results confirmed the presence of Rhipicephalus (Boophilus) annulatus on Cervus elaphus maral representing the first tick infestation record of the Caspian red deer in Iran. The results suggest a possible establishment of this vector species in a wildlife environment.
{"title":"The Caspian red deer, Cervus elaphus maral (Mammalia: Cervidae): a new host record for Rhipicephalus ( Boophilus ) annulatus (Acari: Ixodidae) in northern Iran","authors":"Asadollah Hosseini Chegeni, F. Faghihi, M. Sharifdini, Z. Telmadarraiy","doi":"10.22073/PJA.V10I4.68717","DOIUrl":"https://doi.org/10.22073/PJA.V10I4.68717","url":null,"abstract":"Thirty tick samples were collected from a Caspian red deer in forested areas of Talesh County, Lisar protected area, Gilan province, northern Iran. Tick samples were examined according to morphological characters. In order to specific identification, a fragment (ca. 800-bp) of internal transcribed spacer 2 (ITS2) and a 340-bp fragment of mitochondrial large subunit ribosomal RNA (16S rRNA) gene were amplified by PCR. Nucleotide sequences were analyzed with BLASTn in GenBank. Adult tick samples were identified as female of the genus (subgenus): Rhipicephalus (Boophilus). The results of BLASTn showed that the female ticks belonged to the species Rhipicephalus (Boophilus) annulatus. Our results confirmed the presence of Rhipicephalus (Boophilus) annulatus on Cervus elaphus maral representing the first tick infestation record of the Caspian red deer in Iran. The results suggest a possible establishment of this vector species in a wildlife environment.","PeriodicalId":37567,"journal":{"name":"Persian Journal of Acarology","volume":"10 1","pages":"517-521"},"PeriodicalIF":1.3,"publicationDate":"2021-10-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43188445","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-10-15DOI: 10.22073/PJA.V10I4.67440
A. Khalili-Moghadam, Alrieza Saboori
The mite family Ameroseiidae with 138 valid species is presently classified in the superfamily Ascoidea of the order Mesostigmata. Seventy-two species have been described based on type specimens and no report has been done since then, while the other 66 species have been frequently reported from around the world. The objective of this study is to provide information about distribution and habitat of the latter group, which are listed in twelve genera: Ameroseius (21 species), Kleemannia (15), Neocypholaelaps (8), Epicriopsis (7), Hattena and Sertitympanum (4 each), Ameroseiella (2), Afrocypholaelaps, Asperolaelaps, Brontispalaelaps, Pseudoameroseius and Sinoseius (1 each).
{"title":"World distribution and habitat scope of Ameroseiidae (Acari: Mesostigmata)","authors":"A. Khalili-Moghadam, Alrieza Saboori","doi":"10.22073/PJA.V10I4.67440","DOIUrl":"https://doi.org/10.22073/PJA.V10I4.67440","url":null,"abstract":"The mite family Ameroseiidae with 138 valid species is presently classified in the superfamily Ascoidea of the order Mesostigmata. Seventy-two species have been described based on type specimens and no report has been done since then, while the other 66 species have been frequently reported from around the world. The objective of this study is to provide information about distribution and habitat of the latter group, which are listed in twelve genera: Ameroseius (21 species), Kleemannia (15), Neocypholaelaps (8), Epicriopsis (7), Hattena and Sertitympanum (4 each), Ameroseiella (2), Afrocypholaelaps, Asperolaelaps, Brontispalaelaps, Pseudoameroseius and Sinoseius (1 each).","PeriodicalId":37567,"journal":{"name":"Persian Journal of Acarology","volume":"10 1","pages":"403-450"},"PeriodicalIF":1.3,"publicationDate":"2021-10-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49661063","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-07-15DOI: 10.22073/PJA.V10I3.68875
Lesia M Bondareva, Рetro Ya. Chumak
We have determined the species composition of Eriophyoidea mites on plants of the Pyrus L. genus on the territory of the Academician A.V. Fomin Botanical Garden. It has been found that Eriophyes pyri Pgst and Epitrimerus pyri Nal. dominate. For the first time, individuals of Epitrimerus marginemtorguens Nal., have been found on pear leaves. The sequence of colonization of Eriophyes pyri leaves on a growing shoot of a pear has been determined, which is of practical importance for carrying out protective measures. The level of infestation of Pyrus communus varieties by phytophagous mites has been established. Twenty-seven varieties had low and medium damage. Seven varieties of P. communus and P. salicifolia have been classified as the most damaged by Eriophyes pyri. Along with this, four summer varieties have been very slightly damaged. These data should be taken into account for the monitoring of pest mites and planned population control measures
{"title":"Eriophyoidea mites (Acari: Prostigmata) on common pear ( Pyrus communis L. ): species diversity and varietal attractiveness in the Fomin Botanical Garden (Kyiv, Ukraine)","authors":"Lesia M Bondareva, Рetro Ya. Chumak","doi":"10.22073/PJA.V10I3.68875","DOIUrl":"https://doi.org/10.22073/PJA.V10I3.68875","url":null,"abstract":"We have determined the species composition of Eriophyoidea mites on plants of the Pyrus L. genus on the territory of the Academician A.V. Fomin Botanical Garden. It has been found that Eriophyes pyri Pgst and Epitrimerus pyri Nal. dominate. For the first time, individuals of Epitrimerus marginemtorguens Nal., have been found on pear leaves. The sequence of colonization of Eriophyes pyri leaves on a growing shoot of a pear has been determined, which is of practical importance for carrying out protective measures. The level of infestation of Pyrus communus varieties by phytophagous mites has been established. Twenty-seven varieties had low and medium damage. Seven varieties of P. communus and P. salicifolia have been classified as the most damaged by Eriophyes pyri. Along with this, four summer varieties have been very slightly damaged. These data should be taken into account for the monitoring of pest mites and planned population control measures","PeriodicalId":37567,"journal":{"name":"Persian Journal of Acarology","volume":"10 1","pages":"351–357-351–357"},"PeriodicalIF":1.3,"publicationDate":"2021-07-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46847808","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-07-15DOI: 10.22073/PJA.V10I3.66756
M. M. Ahmed, Hala R. Abdel-Rahman, Mohammed Ali Abdelwines
Knowing pesticides' side-effects on non-target beneficial phytoseiids is crucial for integrated mite management (IMM) programs. The present study investigates the sublethal effects of three fungicides: previcur N (Propamocarb-Hydrochloride 72.2%), penazol 10% EC (Penconazol) and sumi-eight 5% EC (Diniconazole); two insecticides: confidor 20% SL (Imidacloprid) and penny 9% SC (Emamectin benzoate 1.5% & Indoxacarb 7.5%), and two common acaricides: agnar (Spirodiclofen 18% + Abamectin 2%) and biomectin 5% EC (Abamectin) on the predatory mite, Phytoseiulus persimilis Athias-Henriot under both laboratory and greenhouse conditions. The toxicity bioassay and greenhouse experiments revealed that the effects of agnar, confidor and previcur N on P. persimilis is less than other compounds. Therefore, they were selected to evaluate their influence on the population parameters of P. persimilis. Based on the age-stage two-sex life table theory, the LC25 of tested pesticides significantly reduced the longevity, life span and total fecundity of female P. persimilis. Also, it reduced the net (R0) and gross (GRR) reproductive rates. Consequently, the intrinsic (r) and finite (λ) rates of increase were affected. In conclusion, the selected pesticides should not be used with P. persimilis in integrated pest management programs due to their unfavorable effects on the population parameters of this predatory mite.
{"title":"Application of demographic analysis for assessing effects of pesticides on the predatory mite, Phytoseiulus persimilis (Acari: Phytoseiidae)","authors":"M. M. Ahmed, Hala R. Abdel-Rahman, Mohammed Ali Abdelwines","doi":"10.22073/PJA.V10I3.66756","DOIUrl":"https://doi.org/10.22073/PJA.V10I3.66756","url":null,"abstract":"Knowing pesticides' side-effects on non-target beneficial phytoseiids is crucial for integrated mite management (IMM) programs. The present study investigates the sublethal effects of three fungicides: previcur N (Propamocarb-Hydrochloride 72.2%), penazol 10% EC (Penconazol) and sumi-eight 5% EC (Diniconazole); two insecticides: confidor 20% SL (Imidacloprid) and penny 9% SC (Emamectin benzoate 1.5% & Indoxacarb 7.5%), and two common acaricides: agnar (Spirodiclofen 18% + Abamectin 2%) and biomectin 5% EC (Abamectin) on the predatory mite, Phytoseiulus persimilis Athias-Henriot under both laboratory and greenhouse conditions. The toxicity bioassay and greenhouse experiments revealed that the effects of agnar, confidor and previcur N on P. persimilis is less than other compounds. Therefore, they were selected to evaluate their influence on the population parameters of P. persimilis. Based on the age-stage two-sex life table theory, the LC25 of tested pesticides significantly reduced the longevity, life span and total fecundity of female P. persimilis. Also, it reduced the net (R0) and gross (GRR) reproductive rates. Consequently, the intrinsic (r) and finite (λ) rates of increase were affected. In conclusion, the selected pesticides should not be used with P. persimilis in integrated pest management programs due to their unfavorable effects on the population parameters of this predatory mite.","PeriodicalId":37567,"journal":{"name":"Persian Journal of Acarology","volume":"10 1","pages":"281–298-281–298"},"PeriodicalIF":1.3,"publicationDate":"2021-07-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47582838","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-07-15DOI: 10.22073/PJA.V10I3.67125
Mahnaz Kohansal, J. Noei, S. Ramroodi, A. Mokhtari
The members of the genus Cicaditrombium Saboori & Lazarboni originally described from Iran, are known as parasites of Hemiptera (Cicadidae) and Lepidoptera (Noctuidae). Larvae of Cicaditrombium lorestanensis Noei were collected at South Khorasan Province ectoparasitic on Chrysolina sp. (Coleoptera: Chrysomelidae) and Monosteria sp. (Hemiptera: Tingidae), which represent the first known hosts for this species. Larvae of Achaemenothrombium saboorii were collected at South Khorasan Province, Iran ectoparasitic on Erebidae, Noctuidae, Nymphalidae (Lepidoptera); the family Nymphalidae is recorded as a new host. A hitherto undescribed larva belonging to the family Neothrombiidae was found parasitizing Rhipicephalus sanguineus. This is the first record of a member of the family Ixodidae (Acari: Parasitiformes: Ixodida) serving as host for a parasitengone larva. Additional metric and meristic data are provided for C. lorestanensis and A. saboorii.
{"title":"New data on Cicaditrombium lorestanensis Noei, 2017 (Acari: Trombidiidae), Achaemenothrombium saboorii Noei, 2021 (Acari: Achaemenothrombiidae) and the family Neothrombiidae","authors":"Mahnaz Kohansal, J. Noei, S. Ramroodi, A. Mokhtari","doi":"10.22073/PJA.V10I3.67125","DOIUrl":"https://doi.org/10.22073/PJA.V10I3.67125","url":null,"abstract":"The members of the genus Cicaditrombium Saboori & Lazarboni originally described from Iran, are known as parasites of Hemiptera (Cicadidae) and Lepidoptera (Noctuidae). Larvae of Cicaditrombium lorestanensis Noei were collected at South Khorasan Province ectoparasitic on Chrysolina sp. (Coleoptera: Chrysomelidae) and Monosteria sp. (Hemiptera: Tingidae), which represent the first known hosts for this species. Larvae of Achaemenothrombium saboorii were collected at South Khorasan Province, Iran ectoparasitic on Erebidae, Noctuidae, Nymphalidae (Lepidoptera); the family Nymphalidae is recorded as a new host. A hitherto undescribed larva belonging to the family Neothrombiidae was found parasitizing Rhipicephalus sanguineus. This is the first record of a member of the family Ixodidae (Acari: Parasitiformes: Ixodida) serving as host for a parasitengone larva. Additional metric and meristic data are provided for C. lorestanensis and A. saboorii.","PeriodicalId":37567,"journal":{"name":"Persian Journal of Acarology","volume":"10 1","pages":"239-247"},"PeriodicalIF":1.3,"publicationDate":"2021-07-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41713952","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-07-15DOI: 10.22073/PJA.V10I3.68341
Milad Davari, M. Hakimitabar, Asadollah Hosseini Chegeni
Molecular detection techniques based on conventional PCR and sequencing of the partial genome of living organisms are considered an important tool for identification. BLASTn and comparison of evolutionary relationships between taxa are the next analysis steps on sequences. The construction of a phylogenetic tree is done using genetic distance difference; nucleotide substitution models and comparison with the outgroup. In the present study, two samples of tick larvae were collected in Mirafzal forest area (36°07'39.0"N 53°35'45.0"E) located in Mazandaran province, northern Iran using the Berlese funnel. Tick samples were initially identified using a generic identification key (Bregetova et al. 1955) and in order to determine specific identification of species, PCR of partial internal transcribed spacer 2 (ITS2) gene and Sanger sequencing were performed. Morphological results showed that tick samples belonged to Haemaphysalis genus because they had a relatively short capitulum, no eyes with festoons. DNA extraction, PCR amplification and sequencing of a partial fragment (ca. 1400-bp) ITS2 gene of tick sample were done, successfully. The results of BLASTn showed that the larvae belonged to the species Haemaphysalis sulcata. A nucleotide DNA sequence of ITS2 was submitted to GenBank under the accession number MW929218. The molecular technique of this study is recommended to identify immature and dead tick samples that cannot be reared until the adult stage. The present study was the first report on the identification of H. sulcata larvae isolated from the Berlese funnel. The result of this study will help to better understand the biology of this tick and the presence of immature larval stages with questing behaviour in the soil environment.
基于传统PCR和生物部分基因组测序的分子检测技术被认为是鉴定的重要工具。BLASTn和类群间进化关系的比较是下一步序列分析的步骤。利用遗传距离差构建系统发育树;核苷酸取代模型及其与外群的比较。在伊朗北部Mazandaran省Mirafzal森林地区(36°07′39.0”N 53°35′45.0”E)，采用Berlese漏斗采集了2只蜱幼虫。蜱类样本最初使用通用鉴定密钥(Bregetova et al. 1955)进行鉴定，为了确定物种的特异性鉴定，进行了部分内部转录间隔2 (ITS2)基因的PCR和Sanger测序。形态学结果表明，蜱属血蜱，头状花序较短，眼无花。成功提取蜱虫ITS2基因部分片段(约1400 bp)，并进行PCR扩增和测序。BLASTn检测结果表明，该幼虫属血蜱(haemaphyysalis sulcata)。ITS2的核苷酸DNA序列提交到GenBank，登录号为MW929218。本研究的分子技术被推荐用于鉴定未成熟和死亡的蜱虫样本，这些样本不能饲养到成虫阶段。本研究首次报道了从Berlese漏斗中分离的sulata幼虫的鉴定。本研究的结果将有助于更好地了解这种蜱的生物学和未成熟幼虫在土壤环境中具有探索行为的存在。
{"title":"Molecular identification of Haemaphysalis sulcata (Acari: Ixodidae) larval stages collected using the Berlese funnel in Northern Iran","authors":"Milad Davari, M. Hakimitabar, Asadollah Hosseini Chegeni","doi":"10.22073/PJA.V10I3.68341","DOIUrl":"https://doi.org/10.22073/PJA.V10I3.68341","url":null,"abstract":"Molecular detection techniques based on conventional PCR and sequencing of the partial genome of living organisms are considered an important tool for identification. BLASTn and comparison of evolutionary relationships between taxa are the next analysis steps on sequences. The construction of a phylogenetic tree is done using genetic distance difference; nucleotide substitution models and comparison with the outgroup. In the present study, two samples of tick larvae were collected in Mirafzal forest area (36°07'39.0\"N 53°35'45.0\"E) located in Mazandaran province, northern Iran using the Berlese funnel. Tick samples were initially identified using a generic identification key (Bregetova et al. 1955) and in order to determine specific identification of species, PCR of partial internal transcribed spacer 2 (ITS2) gene and Sanger sequencing were performed. Morphological results showed that tick samples belonged to Haemaphysalis genus because they had a relatively short capitulum, no eyes with festoons. DNA extraction, PCR amplification and sequencing of a partial fragment (ca. 1400-bp) ITS2 gene of tick sample were done, successfully. The results of BLASTn showed that the larvae belonged to the species Haemaphysalis sulcata. A nucleotide DNA sequence of ITS2 was submitted to GenBank under the accession number MW929218. The molecular technique of this study is recommended to identify immature and dead tick samples that cannot be reared until the adult stage. The present study was the first report on the identification of H. sulcata larvae isolated from the Berlese funnel. The result of this study will help to better understand the biology of this tick and the presence of immature larval stages with questing behaviour in the soil environment.","PeriodicalId":37567,"journal":{"name":"Persian Journal of Acarology","volume":"10 1","pages":"335–339-335–339"},"PeriodicalIF":1.3,"publicationDate":"2021-07-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48568691","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-07-15DOI: 10.22073/PJA.V10I3.67563
T. Chatterjee
A compilation of deep-sea halacarid mite species found from more than 1000 m depth has been carried out based on published records. Altogether 57 species belong to genera Agaue (6 species), Agauides (1 species), Atelopsalis (1 species), Bathyhalacarus (11 species), Bradyagaue (3 species), Colobocerasides (1 species), Copidognathus (16 species), Halacarellus (2 species), Halacarus (3 species), Lohmannella (5 species), Pelacarus (1 species), Thalassarachna (2 species), Werthella (4 species) and Werthelloides (1 species) are enlisted from deep-sea more than 1000 m depth. Forty-eight species are determined up to species level while 9 species determined up to generic level only. The richness of halacarid mites from more than 1000 m depth in different marine provinces is most likely related to the intensity of faunistic surveys.
{"title":"A checklist of deep-sea halacarid mites (Acari, Halacaridae) found from more than 1000 m depth","authors":"T. Chatterjee","doi":"10.22073/PJA.V10I3.67563","DOIUrl":"https://doi.org/10.22073/PJA.V10I3.67563","url":null,"abstract":"A compilation of deep-sea halacarid mite species found from more than 1000 m depth has been carried out based on published records. Altogether 57 species belong to genera Agaue (6 species), Agauides (1 species), Atelopsalis (1 species), Bathyhalacarus (11 species), Bradyagaue (3 species), Colobocerasides (1 species), Copidognathus (16 species), Halacarellus (2 species), Halacarus (3 species), Lohmannella (5 species), Pelacarus (1 species), Thalassarachna (2 species), Werthella (4 species) and Werthelloides (1 species) are enlisted from deep-sea more than 1000 m depth. Forty-eight species are determined up to species level while 9 species determined up to generic level only. The richness of halacarid mites from more than 1000 m depth in different marine provinces is most likely related to the intensity of faunistic surveys.","PeriodicalId":37567,"journal":{"name":"Persian Journal of Acarology","volume":"10 1","pages":"249–268-249–268"},"PeriodicalIF":1.3,"publicationDate":"2021-07-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45435599","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-07-15DOI: 10.22073/PJA.V10I3.65092
Rumpa Basak, M. Akter, Toufica Ahmed Tumpa, D. Sharmin, M. S. Ullah
The two-spotted spider mite, Tetranychus urticae Koch (Acari: Tetranychidae) is a serious pest that infests different agricultural crops in Bangladesh. The application of different types of pesticides (botanical and chemical) with various modes of action and bio-pesticides was used for the first time to develop sustainable pest control strategies against spider mites. In this study, the effects of six chemical insecticides viz. abamectin, bifenthrin, bifenazate, etoxazole, hexythiazox, and spinosad, and entomopathogenic fungus Beauveria bassiana and botanical neem oil were evaluated against adult and egg stages of T. urticae under laboratory conditions. Treated mites were kept in controlled conditions at 25 ± 1 ℃, 60–70% RH and a photoperiod of 16:8 h (L: D). The LC50 values of all chemicals [for adults, abamectin (0.51 ml/L), bifenthrin (3.26 ml/L), bifenazate (3.82 ml/L), hexythiazox (3.27 ml/L) and spinosad (3.83 ml/L); for eggs, abamectin (0.56 ml/L), bifenthrin (3.48 ml/L), bifenazate (26.81 ml/L), hexythiazox (4.18 ml/L) and spinosad (18.28 ml/L)] suggest that they were effective against eggs and adult females of the T. urticae. Etoxazole (LC50 = 3.99 ml/L) is recommended against egg stage. The neem oil was found to be moderately effective (for adult, LC50 = 1.26%; for egg, LC50 = 1.77%) against eggs and adult females of T. urticae. The B. bassiana was also found to be effective against eggs and adult females of T. urticae. Finally, it could be concluded that all chemicals used in this study are effective for the management of two spotted spider mite but the entomopathogenic fungus B. bassiana and botanical neem oil were promising alternatives for successful management of T. urticae in IPM programs.
{"title":"Laboratory bioassay of six pesticides, an entomopathogenic fungus, and a botanical pesticide on two-spotted spider mite, Tetranychus urticae Koch (Acari: Tetranychidae)","authors":"Rumpa Basak, M. Akter, Toufica Ahmed Tumpa, D. Sharmin, M. S. Ullah","doi":"10.22073/PJA.V10I3.65092","DOIUrl":"https://doi.org/10.22073/PJA.V10I3.65092","url":null,"abstract":"The two-spotted spider mite, Tetranychus urticae Koch (Acari: Tetranychidae) is a serious pest that infests different agricultural crops in Bangladesh. The application of different types of pesticides (botanical and chemical) with various modes of action and bio-pesticides was used for the first time to develop sustainable pest control strategies against spider mites. In this study, the effects of six chemical insecticides viz. abamectin, bifenthrin, bifenazate, etoxazole, hexythiazox, and spinosad, and entomopathogenic fungus Beauveria bassiana and botanical neem oil were evaluated against adult and egg stages of T. urticae under laboratory conditions. Treated mites were kept in controlled conditions at 25 ± 1 ℃, 60–70% RH and a photoperiod of 16:8 h (L: D). The LC50 values of all chemicals [for adults, abamectin (0.51 ml/L), bifenthrin (3.26 ml/L), bifenazate (3.82 ml/L), hexythiazox (3.27 ml/L) and spinosad (3.83 ml/L); for eggs, abamectin (0.56 ml/L), bifenthrin (3.48 ml/L), bifenazate (26.81 ml/L), hexythiazox (4.18 ml/L) and spinosad (18.28 ml/L)] suggest that they were effective against eggs and adult females of the T. urticae. Etoxazole (LC50 = 3.99 ml/L) is recommended against egg stage. The neem oil was found to be moderately effective (for adult, LC50 = 1.26%; for egg, LC50 = 1.77%) against eggs and adult females of T. urticae. The B. bassiana was also found to be effective against eggs and adult females of T. urticae. Finally, it could be concluded that all chemicals used in this study are effective for the management of two spotted spider mite but the entomopathogenic fungus B. bassiana and botanical neem oil were promising alternatives for successful management of T. urticae in IPM programs.","PeriodicalId":37567,"journal":{"name":"Persian Journal of Acarology","volume":"10 1","pages":"269–280-269–280"},"PeriodicalIF":1.3,"publicationDate":"2021-07-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44024703","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-07-15DOI: 10.22073/PJA.V10I3.66732
S. El-Sayed, Heba M. Emam
The pesticide efficacy of propolis (a bee-hive product) was studied on the different stages of the two-spotted spider mite, Tetranychus urticae, under greenhouse conditions. Five concentrations of ethanolic extract of propolis 250, 500, 1000, 1500, 2000 ppm and the control were used in bioassay experiments. Results showed highly significant differences between mortality percentages at all concentrations. The mortality percentage at concentration 250 ppm was calculated for all stages (egg, larva, nymph and adult) after 24 hours which recorded 41.21 ± 0.54, 19.47 ± 0.82, 20.83 ± 0.60, and 44.35 ± 0.47 %, respectively. While the mortality percentages at concentration 250 ppm after 48h for egg, larva, and nymph stages of T. urticae were 62.05 ± 0.16, 50.18 ± 0.28, and 56.03 ± 0.28 %. The high mortality percentage was observed after 72h of treatment of all concentrations and all stages. At concentrations of 1500 and 2000 ppm, the mortality percentage reached its maximum in immature stages, which recorded 95.67 ± 0.47 % in the egg stage. Both larva and nymph stages were 93.86 ± 0.28 and 93.36 ± 0.35% at concentration 1500 ppm. Meanwhile, the concentration of 2000 ppm, the mortality rate was 98.27 ± 0.57% for eggs and 98.25 ± 0.37 and 97.42 ± 0.39% for larva and nymph stages in comparing with the control. Our findings showed that the average value of total phenolic content was 2494.4 µg gallic acid equivalents (GAE)/g ethanolic bee glue (propolis) extract. The antioxidant activity was determined using DPPH (2, 2-diphenyl-1-picrylhydrazyl) assay using ascorbic acid as a standard antioxidant. The obtained results exhibited that the ethanolic extract of propolis has a higher scavenging activity (92.99 %) than that of ascorbic acid (87.32 %) at 30 μg/mL.
{"title":"Effect of propolis extract (bee glue) on Tetranychus urticae Koch (Acari: Tetranychidae) under greenhouse conditions","authors":"S. El-Sayed, Heba M. Emam","doi":"10.22073/PJA.V10I3.66732","DOIUrl":"https://doi.org/10.22073/PJA.V10I3.66732","url":null,"abstract":"The pesticide efficacy of propolis (a bee-hive product) was studied on the different stages of the two-spotted spider mite, Tetranychus urticae, under greenhouse conditions. Five concentrations of ethanolic extract of propolis 250, 500, 1000, 1500, 2000 ppm and the control were used in bioassay experiments. Results showed highly significant differences between mortality percentages at all concentrations. The mortality percentage at concentration 250 ppm was calculated for all stages (egg, larva, nymph and adult) after 24 hours which recorded 41.21 ± 0.54, 19.47 ± 0.82, 20.83 ± 0.60, and 44.35 ± 0.47 %, respectively. While the mortality percentages at concentration 250 ppm after 48h for egg, larva, and nymph stages of T. urticae were 62.05 ± 0.16, 50.18 ± 0.28, and 56.03 ± 0.28 %. The high mortality percentage was observed after 72h of treatment of all concentrations and all stages. At concentrations of 1500 and 2000 ppm, the mortality percentage reached its maximum in immature stages, which recorded 95.67 ± 0.47 % in the egg stage. Both larva and nymph stages were 93.86 ± 0.28 and 93.36 ± 0.35% at concentration 1500 ppm. Meanwhile, the concentration of 2000 ppm, the mortality rate was 98.27 ± 0.57% for eggs and 98.25 ± 0.37 and 97.42 ± 0.39% for larva and nymph stages in comparing with the control. Our findings showed that the average value of total phenolic content was 2494.4 µg gallic acid equivalents (GAE)/g ethanolic bee glue (propolis) extract. The antioxidant activity was determined using DPPH (2, 2-diphenyl-1-picrylhydrazyl) assay using ascorbic acid as a standard antioxidant. The obtained results exhibited that the ethanolic extract of propolis has a higher scavenging activity (92.99 %) than that of ascorbic acid (87.32 %) at 30 μg/mL.","PeriodicalId":37567,"journal":{"name":"Persian Journal of Acarology","volume":"10 1","pages":"299–308-299–308"},"PeriodicalIF":1.3,"publicationDate":"2021-07-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42350071","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-07-15DOI: 10.22073/PJA.V10I3.67518
F. Faraji, Hasan Rahmani, M. Zare
According to the database of Demite et al.(2021), about 90 valid species of Phytoseiidae have been recorded for the fauna of Iran. Here, we present a new report on Amblyseius pseudaequipilusWainstein & Abbasova (1974) collected from decayed wood material in Zanjan province, northwest of Iran.Amblyseiuspseudaequipilus’ original description was adequate for identification purposes. However, due to a mistake in adenotaxy, we decided to re-describe the species accompanied by detailed illustrations and a complete set of measurements.
根据Demite et al.(2021)的数据库，在伊朗动物群中记录了约90种有效的植物seiidae。在这里，我们提出了一份关于伪equipilusamblyseius wainstein & Abbasova(1974)的新报告，该报告收集自伊朗西北部Zanjan省的腐烂木材材料。伪equiblyseuspseudoaequipilus的原始描述足以用于鉴定目的。然而，由于腺腺分类的错误，我们决定重新描述物种，并附有详细的插图和一套完整的测量结果。
{"title":"Re-description of Amblyseius pseudaequipilus Wainstein & Abbasova (Acari: Mesostigmata: Phytoseiidae) based on material collected from Iran","authors":"F. Faraji, Hasan Rahmani, M. Zare","doi":"10.22073/PJA.V10I3.67518","DOIUrl":"https://doi.org/10.22073/PJA.V10I3.67518","url":null,"abstract":"According to the database of Demite et al.(2021), about 90 valid species of Phytoseiidae have been recorded for the fauna of Iran. Here, we present a new report on Amblyseius pseudaequipilusWainstein & Abbasova (1974) collected from decayed wood material in Zanjan province, northwest of Iran.Amblyseiuspseudaequipilus’ original description was adequate for identification purposes. However, due to a mistake in adenotaxy, we decided to re-describe the species accompanied by detailed illustrations and a complete set of measurements.","PeriodicalId":37567,"journal":{"name":"Persian Journal of Acarology","volume":"10 1","pages":"347–350-347–350"},"PeriodicalIF":1.3,"publicationDate":"2021-07-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44748113","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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