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Impedance method application for number detection Escherichia coli in molluscs testing by official laboratory 官方实验室应用阻抗法检测软体动物中大肠埃希氏菌的数量
Q3 Engineering Pub Date : 2023-12-20 DOI: 10.21014/actaimeko.v12i4.1672
Marica Egidio, Raffaele Marrone, Maria Grazia Di Paolo, Salvatore Capo, Emanuele Esposito, Maurizio Della Rotonda, Federico Capuano, Y. Proroga, Alma Sardo, A. Mancusi
Bivalve molluscan shellfish (BMS) have an important role in the transmission of some pathogens responsible for food-borne disease because they are filter-feeding animals capable of greatly concentrating certain pathogenic agents (bacteria, viruses, or parasites) present in the water column, like Escherichia coli. The reference method for E. coli testing in bivalves is the most probable number (MPN) method therefore, as this method has some disadvantages, alternative techniques of equivalent accuracy could be used without the drawbacks of the MPN method such as the direct impedance technique. This method is based on the principle that bacteria produce positively or negatively charged end products causing an impedance variation of the medium that can be used to measure their growth. The present study evaluated the efficiency of the direct impedance measuring technique through a series of laboratory tests performed on 6 kg of clams belonging to two different batches. The preliminary obtained data, compared with the MPN reference method and dd-PCR to validate the alternative method, show that the direct impedance technique has the potential to be used in place of TBX culture for confirming E. coli in MPN assays.
双壳贝类(BMS)在传播某些导致食源性疾病的病原体方面发挥着重要作用,因为它们是滤食性动物,能够极大地聚集水体中的某些病原体(细菌、病毒或寄生虫),如大肠杆菌。双壳贝类大肠杆菌检测的参考方法是最可能数(MPN)法,因此,由于这种方法有一些缺点,可以使用具有同等准确度的替代技术,而不存在 MPN 法的缺点,如直接阻抗技术。这种方法的原理是细菌会产生带正电或负电的终产物,导致培养基阻抗变化,从而用来测量细菌的生长情况。本研究通过对属于两个不同批次的 6 公斤蛤蜊进行一系列实验室测试,评估了直接阻抗测量技术的效率。初步获得的数据与 MPN 参考方法和用于验证替代方法的 dd-PCR 进行了比较,结果表明直接阻抗技术有可能取代 TBX 培养,用于在 MPN 检测中确认大肠杆菌。
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引用次数: 0
Using digital methods in active power measurement 在有功功率测量中使用数字方法
Q3 Engineering Pub Date : 2023-12-20 DOI: 10.21014/actaimeko.v12i4.1590
Francisco Alegria
Digitized voltage and current waveforms can be used to estimate active power by processing the obtained samples through two methods: Discrete Integration and Spectral Analysis. The former involves computing the average of the sample-by-sample product of the two waveforms, while the latter uses sine fitting algorithms to estimate the amplitude and initial phase of each waveform. Precision expressions for both estimators are derived as a function of the number of samples acquired and the amount of additive random noise present, which is useful for determining the confidence interval for measurements. The two estimators are compared, and it is concluded that the second method, using sine fitting, is sometimes superior.
数字化的电压和电流波形可用于估算有功功率,方法有两种:离散积分法和频谱分析法:离散积分法和频谱分析法。前者涉及计算两个波形逐个样本乘积的平均值,后者则使用正弦拟合算法来估算每个波形的振幅和初始相位。这两种估算器的精度表达式都是获取的样本数和存在的加性随机噪声量的函数,有助于确定测量的置信区间。对这两种估计方法进行了比较,得出的结论是,使用正弦拟合的第二种方法有时更胜一筹。
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引用次数: 0
Performance evaluation of pocket echo using an ultrasonic accuracy control phantom 利用超声波精度控制模型对袋式回波进行性能评估
Q3 Engineering Pub Date : 2023-12-20 DOI: 10.21014/actaimeko.v12i4.1382
Norimitsu Shinohara, Y. Kondo
In addition to being used in hospitals, ultrasound systems are used in many other medical settings such as disaster relief and home care. In these types of settings, it is important to be able to perform a large number of examinations easily and efficiently. Portable ultrasound systems can be used to meet such needs. The evaluation of ultrasound systems has been driven by the development of accuracy control methods used in breast examinations. This study aimed to evaluate the performance of portable ultrasound systems that have not yet been fully investigated. The performance of two ultrasound systems was evaluated using three measures. For physical evaluation, the change in the mean pixel value of the target and the contrast-to-noise ratio were obtained for each ultrasound system. Statistical analyses were performed to compare these measures between the two systems. For visual evaluation, a receiver operating characteristic analysis was performed. The results of the physical and visual evaluations showed no statistically significant differences between the portable ultrasound systems we evaluated and those that are commonly used in clinical practice.
除了在医院中使用,超声波系统还用于许多其他医疗场所,如救灾和家庭护理。在这些环境中,能够轻松高效地进行大量检查非常重要。便携式超声系统可以满足这些需求。对超声系统进行评估的动力来自于乳腺检查中使用的精确度控制方法的发展。本研究旨在评估尚未得到充分研究的便携式超声系统的性能。研究采用三种方法对两种超声系统的性能进行了评估。在物理评估方面,每个超声系统都获得了目标平均像素值的变化和对比度-噪声比。通过统计分析来比较两种系统的这些指标。在视觉评估方面,则进行了接收器操作特性分析。物理和视觉评估结果表明,我们所评估的便携式超声系统与临床上常用的超声系统在统计学上没有明显差异。
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引用次数: 0
Data augmentation for solving industrial recognition tasks with underrepresented defect classes 利用数据增强技术解决缺陷类别代表性不足的工业识别任务
Q3 Engineering Pub Date : 2023-12-19 DOI: 10.21014/actaimeko.v12i4.1320
Lennard Wunsch, Katharina Anding, Galina Polte, Kun Liu, Gunther Notni
This paper discusses neural network-based data augmentation to increase the performance of neural networks in classification of datasets with underrepresented defect classes. The performance of deep neural networks suffers from an inhomogeneous class distribution in recognition tasks. In particular, applications of deep neural networks to solve quality assurance tasks in industrial production suffer from such unbalanced class distributions. In order to train deep learning networks, a large amount of data is needed to avoid overfitting and to give the network a good generalisation ability. Therefore, a large amount of defect class objects is needed. However, when it comes to producing defect classes, obtaining a dataset for training can be costly. To reduce this costs, artificial intelligence in the form of Generative Adversarial Networks (GANs) can be used to generate images without producing real objects of defect classes. This allows a cost-effective solution for any kind of underrepresented classes. However, the focus of this work is on defect classes. In this paper a comparison of GANs for data augmentation with classical data augmentation methods for simulating images of defect classes in an industrial context is presented. The results show the positive effect of both, classical and GAN-based data augmentation. By applying both methods parallel the best results for defect-class recognition tasks of datasets with underrepresented classes can be achieved.
本文讨论了基于神经网络的数据增强,以提高神经网络在缺陷类别代表性不足的数据集分类中的性能。在识别任务中,深度神经网络的性能会受到类别分布不均的影响。特别是,深度神经网络在解决工业生产中的质量保证任务时,就会受到这种不均衡类别分布的影响。为了训练深度学习网络,需要大量数据以避免过拟合,并使网络具有良好的泛化能力。因此,需要大量的缺陷类对象。然而,在生成缺陷类时,获取用于训练的数据集的成本可能会很高。为了降低成本,可以使用生成对抗网络(GAN)形式的人工智能来生成图像,而无需生成真实的缺陷类对象。这就为任何类型的代表性不足的类别提供了一种具有成本效益的解决方案。不过,这项工作的重点是缺陷类别。本文比较了用于数据增强的 GAN 与用于模拟工业缺陷类别图像的经典数据增强方法。结果表明,经典数据扩增和基于 GAN 的数据扩增都有积极的效果。通过并行应用这两种方法,可以在具有代表性不足类别的数据集的缺陷类别识别任务中取得最佳结果。
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引用次数: 0
Image uncertainty components in video strain measurement of mortar destructive compression testing 砂浆破坏性压缩试验视频应变测量中的图像不确定性成分
Q3 Engineering Pub Date : 2023-12-19 DOI: 10.21014/actaimeko.v12i4.1315
L. Martins, Ana Isabel Marques, Álvaro Silva Ribeiro
This paper describes a video strain measurement solution developed for application in mortar destructive compression testing. Knowledge about the mechanical behavior of this type of construction material, namely up to its fracture, is still superficial due to the less common use of non-contact measurement methods. The performed research was focused on the determination of image coordinate accuracy, based on the experimental quantification of the identified main uncertainty components, using traceable reference patterns, and validated computational toolboxes dedicated to camera parameterization and digital image processing. The obtained results show the following uncertainty contributions: lens distortion - negligible; re-projection errors - 0.21 pixel; 0.10 pixel - spatial resolution; and digital image processing operations - 0.28 pixel. The combination of these uncertainty components resulted in an image coordinate standard uncertainty equal to 0.36 pixel, which was propagated (in addition to the scale coefficient measurement uncertainty) to the selected camera model – orthographic projection with uniform scaling – which supports the video strain measurement.
本文介绍了一种应用于砂浆破坏性压缩试验的视频应变测量解决方案。由于较少使用非接触式测量方法,对这类建筑材料的机械行为(即直至断裂)的了解还很肤浅。所进行的研究主要集中在图像坐标精度的确定上,其基础是使用可溯源的参考模式和经过验证的专用于相机参数化和数字图像处理的计算工具箱,对已确定的主要不确定性成分进行实验量化。所得结果显示了以下不确定性因素:镜头畸变--可忽略不计;重投影误差--0.21 像素;空间分辨率--0.10 像素;数字图像处理操作--0.28 像素。这些不确定因素的组合导致图像坐标标准不确定度为 0.36 像素,该不确定度(除比例系数测量不确定度外)已传播到所选的摄像机模型--具有均匀缩放的正投影模型--该模型支持视频应变测量。
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引用次数: 0
Entropy-based explanations of serial position and learning effects in ordinal responses to word list tests 基于熵的词表测试顺序反应中序列位置和学习效应的解释
Q3 Engineering Pub Date : 2023-12-19 DOI: 10.21014/actaimeko.v12i4.1314
J. Melin, Petronella Kettunen, Anders Wallin, L. Pendrill
Measuring a person’s cognitive abilities, such as memory and learning, is central in many medical conditions to reliably diagnose, treat and monitor disease progression. Common tests typically include tasks of recalling sequences of blocks, digits or words. Recalling a word list is affected by so-called serial position effects (SPE), meaning that words at the beginning or end of the list are more likely to be recalled. In our earlier work, as part of including ordinal and nominal properties in metrology, compensation for ordinality in the raw test scores has been performed with psychometric Rasch measurement theory. Thereafter, SPE have been successfully explained with construct specification equations (CSE) dominated by information theoretical entropy as candidate reference measurement procedures. Here, we present how previous German results for explaining memory difficulty in the immediate recalling (IR, trial 1) task of the Rey’s Auditory Verbal Learning Test (RAVLT) can be replicated with a Swedish cohort (the Gothenburg Mild Cognitive Impairment study, n = 251). This CSE replicability for RAVLT demonstrates comparability across the two cohorts in a kind of inter-laboratory study. Moreover, RAVLT includes repeated trials and learning through practice is expected. How memory task difficulty changes over the eight trials in RAVLT is studied: SPE are not so prominent for the delayed recalling sequences and there is an overall reduction in the task difficulty CSE intercept with trial number, interpreted as an effect of learning. To conclude, the methodology and evidence provided here can be clinically used not only to measure a person’s memory ability but also his or her learning ability, as well as understanding the relationship between learning ability and other cognitive domains.
测量一个人的认知能力,如记忆力和学习能力,是许多医疗条件下可靠诊断、治疗和监测疾病进展的核心。常见的测试通常包括回忆方块、数字或单词序列的任务。单词表的回忆会受到所谓的序列位置效应(SPE)的影响,即位于单词表开头或结尾的单词更容易被回忆起来。在我们早期的工作中,作为计量学中序数和标称属性的一部分,我们利用心理测量学 Rasch 测量理论对原始测试分数的序数性进行了补偿。此后,我们又成功地利用以信息论熵为主导的构造规范方程(CSE)作为候选参考测量程序,对 SPE 进行了解释。在此,我们介绍了德国之前对雷氏听觉言语学习测验(RAVLT)中即时回忆(IR,试验 1)任务记忆困难的解释结果如何在瑞典队列(哥德堡轻度认知障碍研究,n = 251)中得到复制。RAVLT 的 CSE 可重复性表明,在一种实验室间研究中,两个队列之间具有可比性。此外,RAVLT 包括重复试验,通过练习学习是可以预期的。在 RAVLT 中,我们研究了记忆任务难度在八次试验中的变化情况:在延迟回忆序列中,SPE 的作用并不突出,任务难度 CSE 截距随着试验次数的增加而整体降低,这可以解释为学习的影响。总之,本文提供的方法和证据不仅可用于临床测量一个人的记忆能力,还可用于测量其学习能力,以及了解学习能力与其他认知领域之间的关系。
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引用次数: 0
The palaeontological heritage of the Capo Vaticano – Monte Poro area (Vibo Valentia, Italy): research, protection, and management 瓦蒂卡诺山-波罗山地区(意大利维博瓦伦西亚)的古生物遗产:研究、保护和管理
Q3 Engineering Pub Date : 2023-12-13 DOI: 10.21014/actaimeko.v12i4.1479
Antonella Cinzia Marra, Roberta Somma, Adriano Guido
The Late Miocene successions of the Capo Vaticano-Monte Poro (Southern Italy) area, well exposed at Cessaniti – Cava Gentile, preserve a palaeontological record particularly relevant for phylogenetic studies and paleogeographic reconstructions as well as for the popular scientific divulgation and museology. The rich record of Sirenians highly contributed to the knowledge of phylogeny and intraspecific variability of Metaxytherium serresii. The exclusive terrestrial mammal association, having African and Greco-Iranian affinities, allows intriguing suggestions in the paleogeography of the Central Mediterranean. New taphonomic analyses on invertebrates and vertebrates, here presented, suggest that the good fossil preservation is due to the rapid burial of skeletons in a semipermeable mixture of sandy/muddy sediments which were quickly cemented. Late partial dissolution of the carbonate cement among particles made the fossils easy to extract from the sediment. The collaboration among palaeontology researchers, fossil collectors, and local administrators, under the direction of Superintendence and the control of Carabinieri Command for the Protection of Cultural Heritage, allowed the recovery, study, and promotion of this precious paleontological record. The area has a good flow of tourists for the pleasant seaside, and fossiliferous sites could attract cultural tourism, through educational trails and a museum network which valorize the palaeontological and archaeological heritage of the hinterland.
梵蒂冈角-蒙特波罗(意大利南部)地区的晚中新世演替在 Cessaniti - Cava Gentile 出露较好,保存了与系统发育研究和古地理重建以及大众科学传播和博物馆学特别相关的古生物记录。丰富的塞壬类记录极大地促进了对 Metaxytherium serresii 系统发育和种内变异的了解。这种独特的陆生哺乳动物与非洲和希腊-伊朗的亲缘关系,为地中海中部的古地理学提供了有趣的建议。对无脊椎动物和脊椎动物进行的新的岩石学分析表明,化石保存完好的原因是骨骼被迅速埋藏在半透性的砂质/泥质混合沉积物中,并很快被固结。颗粒间碳酸盐胶结物的后期部分溶解使得化石很容易从沉积物中提取出来。古生物学研究人员、化石采集者和当地管理者在监管局的指导和保护文化遗产宪兵司令部的控制下通力协作,使得这一珍贵的古生物记录得以恢复、研究和推广。该地区宜人的海滨风光吸引了大量游客,而化石遗址则可以通过教育路径和博物馆网络吸引文化旅游,从而提升腹地古生物学和考古学遗产的价值。
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引用次数: 0
Reliable use of smart cameras for monitoring biometric parameters in buffalo precision livestock farming 在水牛精准畜牧业中可靠使用智能相机监测生物特征参数
Q3 Engineering Pub Date : 2023-12-12 DOI: 10.21014/actaimeko.v12i4.1638
R. Matera, Leopoldo Angrisani, Gianluca Neglia, Angela Salzano, F. Bonavolontã, Maria Teresa Verde, N. Piscopo, Domenico Vistocco, Oscar Tamburis
Precision Livestock Farming, as a specific sub-sector of Public Health Informatics, focuses on the application of process engineering principles and techniques to achieve an automatic monitoring, modelling, and management of animal productions. In the present work a timely "protocol" is proposed for unobtrusive direct/indirect monitoring of biometric parameters for the estimation of body conditions on Mediterranean Buffalo populations, using low-cost automated systems already present on the market i.e., smart cameras endowed with depth perception capabilities.
精准畜牧业是公共卫生信息学的一个特定子领域,其重点是应用过程工程原理和技术,实现对动物生产的自动监测、建模和管理。在本项工作中,提出了一项及时的 "协议",利用市场上已有的低成本自动化系统,即具有深度感知能力的智能相机,对生物计量参数进行非侵入式直接/间接监测,以估计地中海水牛的身体状况。
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引用次数: 0
Evaluation of spatial patterns accuracy in identifying built-up areas within risk zones using deep learning, RGB aerial imagery, and multi-source GIS data 利用深度学习、RGB 航空图像和多源 GIS 数据识别风险区内建筑密集区的空间模式准确性评估
Q3 Engineering Pub Date : 2023-12-12 DOI: 10.21014/actaimeko.v12i4.1708
A. Vitale, Carolina Salvo, Francesco Lamonaca
In the presence of natural disasters that increasingly affect urban centers, innovative methodologies that can support all the subjects and bodies involved in the disaster management system are increasingly important. This task can be enhanced in urban settings by automatically assessing at-risk buildings through satellite and aerial imagery. However, creating and implementing models with robust generalization capabilities is crucial to achieving this goal. Based on these premises, the authors proposed a deep learning approach utilizing the U-Net model to map buildings within known landslide-prone areas. They trained and validated the U-Net model using the Dubai Satellite Imagery Dataset. The model's prediction accuracy in adapting its results to urban environments in Italy, different from those involved in the training and validation stages, was tested using natural color orthoimages and diverse geographic information system (GIS) data sources. The outcomes indicate that the model's predictions are better in contexts with denser urban fabric. The level of accuracy in dispersed urban shapes worsens as building footprints cover a small portion of the total image area. Overall, the results demonstrate that the suggested methodology can effectively identify buildings in landslide risk zones, demonstrating noteworthy adaptability, making the proposed platform a tool that can be instrumental for decision-makers and urban planners in pre-disaster and post-disaster stages.
在自然灾害日益影响城市中心的情况下,能够为灾害管理系统所涉及的所有主体和机构提供支持的创新方法变得越来越重要。在城市环境中,通过卫星和航空图像自动评估有风险的建筑物可以加强这项任务。然而,要实现这一目标,创建和实施具有强大概括能力的模型至关重要。基于这些前提,作者提出了一种深度学习方法,利用 U-Net 模型来绘制已知滑坡易发区内的建筑物。他们使用迪拜卫星图像数据集对 U-Net 模型进行了训练和验证。他们使用自然彩色正射影像和各种地理信息系统(GIS)数据源,测试了该模型将其结果应用于意大利城市环境的预测准确性。 结果表明,在城市结构较为密集的情况下,模型的预测效果更好。当建筑足迹只占图像总面积的一小部分时,分散的城市形态的准确性就会下降。总之,研究结果表明,所建议的方法可以有效识别滑坡风险区内的建筑物,显示出显著的适应性,使所建议的平台成为决策者和城市规划者在灾前和灾后阶段的有用工具。
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引用次数: 0
Surface tension measurements – A comparative study 表面张力测量 - 对比研究
Q3 Engineering Pub Date : 2023-12-12 DOI: 10.21014/actaimeko.v12i4.1418
Sara Rosa Moura, Andreia Furtado, Olivier Pellegrino, Elźbieta Lenard, Ewa Malejczyk, G. Sariyerli, Umit Y Akcadag, João Sousa
The lack of intercomparisons in surface tension measurements, and the need to establish the degree of equivalence of such measurements, lead to a comparative study on this subject carried out by 3 National Metrology Institutes (from Poland, Portugal, and Turkey), within the scope of the Project 17RPT02-rhoLiq. For this purpose, the surface tension of 6 liquids, was determined at 20 °C, using force tensiometers, with 2 different measuring probes (Du Noüy ring and Wilhelmy plate), and by applying different correction factors. The results of this work allowed to conclude on the degree of equivalence of surface tension measurement results, obtained by each NMI, by using different probes and measurement models.
由于缺乏表面张力测量的相互比较,以及需要确定此类测量的等效程度,3 个国家计量研究院(分别来自波兰、葡萄牙和土耳其)在 17RPT02-rhoLiq 项目范围内就这一主题开展了一项比较研究。为此,使用拉力张力计和两种不同的测量探头(杜诺伊环和威尔海米板),并应用不同的修正系数,测定了 6 种液体在 20 °C 时的表面张力。根据这项工作的结果,可以得出结论:使用不同的探头和测量模型,每种 NMI 所获得的表面张力测量结果的等效程度。
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引用次数: 0
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