Pub Date : 2024-03-29DOI: 10.21014/actaimeko.v13i1.1851
Francesco Lamonaca
{"title":"Introductory notes for the Acta IMEKO first issue in 2024","authors":"Francesco Lamonaca","doi":"10.21014/actaimeko.v13i1.1851","DOIUrl":"https://doi.org/10.21014/actaimeko.v13i1.1851","url":null,"abstract":"","PeriodicalId":37987,"journal":{"name":"Acta IMEKO","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2024-03-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140367249","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-03-29DOI: 10.21014/actaimeko.v13i1.1852
Leopoldo Angrisani, Francesco Lamonaca, Gianluca Neglia
{"title":"Introductory notes to the Thematic issue on Measurements and Applications in Veterinary and Animal Sciences","authors":"Leopoldo Angrisani, Francesco Lamonaca, Gianluca Neglia","doi":"10.21014/actaimeko.v13i1.1852","DOIUrl":"https://doi.org/10.21014/actaimeko.v13i1.1852","url":null,"abstract":"","PeriodicalId":37987,"journal":{"name":"Acta IMEKO","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2024-03-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140368125","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-03-28DOI: 10.21014/actaimeko.v13i1.1743
Ugo Ala, Angela Del Carro, M. Giacobini, Barbara Colitti, Ada Rota, L. Bertolotti
In recent years, the role of the microbiota has proved to be extremely important in medicine as one of the most important aspects for the characterization of living beings in both healthy and pathological conditions. Moreover, the development of shotgun technology, and in particular the cheaper 16S ribosomal RNA (rRNA) gene sequencing, made possible its wide diffusion. In veterinary sciences, microbiome studies have seen applications not only in medicine in the strict sense (e.g diagnosis) but also, for example, in food inspection (quality, fraud, etc.) and in animal feed preparation itself. However, focusing on microbial profiling by 16S rRNA sequencing, there are several crucial aspects to be considered: from the experimental design definition and the sample size problem to the data analysis steps. This latter involves several layers, e.g. which 16S rRNA databases to use, which metrics for alpha and beta diversity, etc. In this work, we want to present, as a case study, a critical discussion about the large number of alpha and beta diversity metrics and their impact in the statistical comparisons among groups.
{"title":"Microbiome studies in veterinary field: communities’ diversity measurements pitfalls","authors":"Ugo Ala, Angela Del Carro, M. Giacobini, Barbara Colitti, Ada Rota, L. Bertolotti","doi":"10.21014/actaimeko.v13i1.1743","DOIUrl":"https://doi.org/10.21014/actaimeko.v13i1.1743","url":null,"abstract":"In recent years, the role of the microbiota has proved to be extremely important in medicine as one of the most important aspects for the characterization of living beings in both healthy and pathological conditions. Moreover, the development of shotgun technology, and in particular the cheaper 16S ribosomal RNA (rRNA) gene sequencing, made possible its wide diffusion. In veterinary sciences, microbiome studies have seen applications not only in medicine in the strict sense (e.g diagnosis) but also, for example, in food inspection (quality, fraud, etc.) and in animal feed preparation itself. However, focusing on microbial profiling by 16S rRNA sequencing, there are several crucial aspects to be considered: from the experimental design definition and the sample size problem to the data analysis steps. This latter involves several layers, e.g. which 16S rRNA databases to use, which metrics for alpha and beta diversity, etc. In this work, we want to present, as a case study, a critical discussion about the large number of alpha and beta diversity metrics and their impact in the statistical comparisons among groups.","PeriodicalId":37987,"journal":{"name":"Acta IMEKO","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2024-03-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140368784","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-03-28DOI: 10.21014/actaimeko.v13i1.1670
Daria Lotito, Gabriella Orazzo, Roberta Matera, Nadia Musco, A. Staropoli, Francesco Vinale
Traditional approaches to protect agricultural crops are based on the use of chemical pesticides. Nevertheless, these products are costly, contaminate the environment and are harmful to animals and humans. Reduction or elimination of chemical applications in agriculture is extremely needed, and the application of biostimulants and biological control agents is an efficient alternative for an eco-sustainable agriculture. This study demonstrated that formulations based on fungi belonging to Trichoderma genus had beneficial effects on forage crops and significantly affected plant metabolome.
{"title":"Beneficial fungal microbes as novel ecosustainable tools for forage crops","authors":"Daria Lotito, Gabriella Orazzo, Roberta Matera, Nadia Musco, A. Staropoli, Francesco Vinale","doi":"10.21014/actaimeko.v13i1.1670","DOIUrl":"https://doi.org/10.21014/actaimeko.v13i1.1670","url":null,"abstract":"Traditional approaches to protect agricultural crops are based on the use of chemical pesticides. Nevertheless, these products are costly, contaminate the environment and are harmful to animals and humans. Reduction or elimination of chemical applications in agriculture is extremely needed, and the application of biostimulants and biological control agents is an efficient alternative for an eco-sustainable agriculture. This study demonstrated that formulations based on fungi belonging to Trichoderma genus had beneficial effects on forage crops and significantly affected plant metabolome.\u0000 ","PeriodicalId":37987,"journal":{"name":"Acta IMEKO","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2024-03-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140369264","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-03-27DOI: 10.21014/actaimeko.v13i1.1643
Irene Diamanti, R. Galarini, Raffaella Branciari, Giorgio Saluti, L. Fioroni
Sulfonamides represent a wide class of synthetic drugs commonly used in veterinary therapy for the treatment of several bacterial and protozoan infections in cattle, swine and poultry. The use of these drugs in farming can lead to the possibility of having their residues in animal products intended for human consumption. Consequently, to ensure high consumer protection, for sulfonamides European Union (EU) set a Maximum Residue Limit (MRL) equal to 100 µg/kg, either as a single molecule or as a sum of all detected compounds within the class). Official laboratories are directly involved in the execution or residue plans by developing, validating and then applying analytical methods for the measurement of drug residues. Accordingly, official laboratories should update their procedures following the evolution of required drugs and MRLs. A multiclass method previously developed and validated for the determination in animal muscle of ten classes of antibiotics was adjusted to comply with the current European requirements which establish the minimum set of sulfonamides to be determined. Therefore, eight new sulfonamides were added assessing method performance characteristics according to European Regulation (EU) 808/2021.
{"title":"Determination of sulfonamides in muscle: a metrological tool for food safety","authors":"Irene Diamanti, R. Galarini, Raffaella Branciari, Giorgio Saluti, L. Fioroni","doi":"10.21014/actaimeko.v13i1.1643","DOIUrl":"https://doi.org/10.21014/actaimeko.v13i1.1643","url":null,"abstract":"Sulfonamides represent a wide class of synthetic drugs commonly used in veterinary therapy for the treatment of several bacterial and protozoan infections in cattle, swine and poultry. The use of these drugs in farming can lead to the possibility of having their residues in animal products intended for human consumption. Consequently, to ensure high consumer protection, for sulfonamides European Union (EU) set a Maximum Residue Limit (MRL) equal to 100 µg/kg, either as a single molecule or as a sum of all detected compounds within the class). Official laboratories are directly involved in the execution or residue plans by developing, validating and then applying analytical methods for the measurement of drug residues. Accordingly, official laboratories should update their procedures following the evolution of required drugs and MRLs. A multiclass method previously developed and validated for the determination in animal muscle of ten classes of antibiotics was adjusted to comply with the current European requirements which establish the minimum set of sulfonamides to be determined. Therefore, eight new sulfonamides were added assessing method performance characteristics according to European Regulation (EU) 808/2021.","PeriodicalId":37987,"journal":{"name":"Acta IMEKO","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2024-03-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140375478","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-03-27DOI: 10.21014/actaimeko.v13i1.1665
Alessandro Vastolo, Dieu Donné Kiatti, Monica Isabella Cutrignelli, S. Calabrò
The aim of this study was to characterize eight mixed forages preserved as hays produced in four agricultural and livestock farms located in four Provinces (Avellino, Benevento, Campobasso, and Potenza) of Southern Italy, different for environmental condition. Nutritive value, in vitro fermentation characteristics and kinetics, including methane production were determined. The little differences observed between sampling areas highlighted that the forage produced in Avellino area is the most interesting in terms of chemical composition, nutritive value, in vitro characteristics, and environmental impact. Data obtained allow having more information about hays produced in the study area, useful for farmers to make balanced rations, to maintain animal health and guarantee high quality of production.
{"title":"Mixed hay produced in Southern Italy: nutritive value and environmental impact","authors":"Alessandro Vastolo, Dieu Donné Kiatti, Monica Isabella Cutrignelli, S. Calabrò","doi":"10.21014/actaimeko.v13i1.1665","DOIUrl":"https://doi.org/10.21014/actaimeko.v13i1.1665","url":null,"abstract":"The aim of this study was to characterize eight mixed forages preserved as hays produced in four agricultural and livestock farms located in four Provinces (Avellino, Benevento, Campobasso, and Potenza) of Southern Italy, different for environmental condition. Nutritive value, in vitro fermentation characteristics and kinetics, including methane production were determined. The little differences observed between sampling areas highlighted that the forage produced in Avellino area is the most interesting in terms of chemical composition, nutritive value, in vitro characteristics, and environmental impact. Data obtained allow having more information about hays produced in the study area, useful for farmers to make balanced rations, to maintain animal health and guarantee high quality of production.\u0000 ","PeriodicalId":37987,"journal":{"name":"Acta IMEKO","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2024-03-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140374128","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-03-27DOI: 10.21014/actaimeko.v13i1.1656
Maria Grazia Di Paolo, Giulia Polizzi, L. Vollano, A. Anastasio, Giovanna Bifulco, C. Lambiase, Alessandro Cuomo, Raffaele Marrone
Texture of meat is a critical parameter of consumer’s acceptability. In this regard, aging technology has become essential to enhance meat tenderness and flavour. The present study evaluated the effect of dry-aging on the rheological properties of cooked and raw meat (Longissiumus dorsi) using objective instrumental measurements: colorimeter (CIEL*a*b*), texture profile analysis (TPA-test) and Warner Bratzler shear force test (WBSF-test). In this study, a monitored refrigeration device was used for 60 days of dry aging of meat. Physical-chemical analyses were also evaluated on raw meat. The analyses were carried out at 2 (T0), 15 (T1), 30 (T2) and 60 (T3) days post-slaughter. During dry aging, aw values tended to decrease while the pH values increased. The mayor color changes occurred in the first 15 days of dry aging. As regard the texture traits, significant differences were found between the cooked and raw meat mainly due to the effect of temperatures on collagen. The correlation data showed significant positive correlations between WBSF and hardness values. Results showed that aging time and cooking tended to decrease the hardness and shear force implying more tender meat for the consumer.
{"title":"Measurement of rheological properties in raw and cooked meat aged with a controlled dry-aging system","authors":"Maria Grazia Di Paolo, Giulia Polizzi, L. Vollano, A. Anastasio, Giovanna Bifulco, C. Lambiase, Alessandro Cuomo, Raffaele Marrone","doi":"10.21014/actaimeko.v13i1.1656","DOIUrl":"https://doi.org/10.21014/actaimeko.v13i1.1656","url":null,"abstract":"Texture of meat is a critical parameter of consumer’s acceptability. In this regard, aging technology has become essential to enhance meat tenderness and flavour. The present study evaluated the effect of dry-aging on the rheological properties of cooked and raw meat (Longissiumus dorsi) using objective instrumental measurements: colorimeter (CIEL*a*b*), texture profile analysis (TPA-test) and Warner Bratzler shear force test (WBSF-test). In this study, a monitored refrigeration device was used for 60 days of dry aging of meat. Physical-chemical analyses were also evaluated on raw meat. The analyses were carried out at 2 (T0), 15 (T1), 30 (T2) and 60 (T3) days post-slaughter. During dry aging, aw values tended to decrease while the pH values increased. The mayor color changes occurred in the first 15 days of dry aging. As regard the texture traits, significant differences were found between the cooked and raw meat mainly due to the effect of temperatures on collagen. The correlation data showed significant positive correlations between WBSF and hardness values. Results showed that aging time and cooking tended to decrease the hardness and shear force implying more tender meat for the consumer.","PeriodicalId":37987,"journal":{"name":"Acta IMEKO","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2024-03-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140374067","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-03-26DOI: 10.21014/actaimeko.v13i1.1719
Elisabetta Porzio, S. Mecocci, Francesco Renzi, Giovanni Chillemi, Marco Milanesi, Giovanni Vignali, Riccardo Valentini, F. Beccati, Elisabetta Chiaradia, Massimo Trabalza-Minnucci, Katia Cappelli, Marco Pepe
Exercise tests are indicated for the evaluation of a horse’s physical condition and for the analysis of poor athletic performance, often associated with discomfort during training or competition that creates excessive stress in the animal. In order to understand the different biological mechanisms of adaptation to exercise-induced stress, a large amount of data need to be collected in real time, to obtain what is called “deep phenotyping” (DEPH) that opens the way to the full exploitation of omic techniques. The aim of the study is the configuration of innovative low-cost sensors for real time detection of crucial stress parameters that will allow early identification of metabolic dysfunctions preserving the horse’s welfare.
{"title":"Innovative sensors for the assessment of exercise stress in athlete horse","authors":"Elisabetta Porzio, S. Mecocci, Francesco Renzi, Giovanni Chillemi, Marco Milanesi, Giovanni Vignali, Riccardo Valentini, F. Beccati, Elisabetta Chiaradia, Massimo Trabalza-Minnucci, Katia Cappelli, Marco Pepe","doi":"10.21014/actaimeko.v13i1.1719","DOIUrl":"https://doi.org/10.21014/actaimeko.v13i1.1719","url":null,"abstract":"Exercise tests are indicated for the evaluation of a horse’s physical condition and for the analysis of poor athletic performance, often associated with discomfort during training or competition that creates excessive stress in the animal. In order to understand the different biological mechanisms of adaptation to exercise-induced stress, a large amount of data need to be collected in real time, to obtain what is called “deep phenotyping” (DEPH) that opens the way to the full exploitation of omic techniques. The aim of the study is the configuration of innovative low-cost sensors for real time detection of crucial stress parameters that will allow early identification of metabolic dysfunctions preserving the horse’s welfare.","PeriodicalId":37987,"journal":{"name":"Acta IMEKO","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2024-03-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140380602","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-03-26DOI: 10.21014/actaimeko.v13i1.1688
Ester Scotto di Perta, E. Cervelli, R. Grieco, Antonio Mautone, S. Pindozzi
Anaerobic digestion can provide a valuable aid to manure management while producing renewable energy. Biogas production is highly dependent on the size and composition of livestock farms, and the availability of fresh manure can vary throughout the year, limiting reliable assessment of potential production. The aim of this study is to develop an affordable GIS-based analysis to support manure management, based on a highly detailed livestock farm database. Databases refer to the years 2013 and 2019 and report the herd consistency and the age of each class head. Kernel density (KD) was used to identify emerging hotspot areas with potentially high concentrations of nitrogen applied to the field. Three KD classification methods were compared: defined interval (DI) into 3 classes (0-170, 170-340, > 340 kg N/ha), quantile (Q) and natural break (NB). The results show that the DI and NB areas correspond to 40 % and 84 % of the total N of buffalo origin in the Campania region, with a N surplus in the hotspot areas localized in nitrate vulnerable zones of 55 and 6 % respectively. The biogas produced from 50 % of the buffalo manure in these areas generates sufficient energy to allow the removal of the N surplus.
{"title":"GIS-based analysis to assess biogas energy potential as support for manure management in Southern Italy","authors":"Ester Scotto di Perta, E. Cervelli, R. Grieco, Antonio Mautone, S. Pindozzi","doi":"10.21014/actaimeko.v13i1.1688","DOIUrl":"https://doi.org/10.21014/actaimeko.v13i1.1688","url":null,"abstract":"Anaerobic digestion can provide a valuable aid to manure management while producing renewable energy. Biogas production is highly dependent on the size and composition of livestock farms, and the availability of fresh manure can vary throughout the year, limiting reliable assessment of potential production. The aim of this study is to develop an affordable GIS-based analysis to support manure management, based on a highly detailed livestock farm database. Databases refer to the years 2013 and 2019 and report the herd consistency and the age of each class head. Kernel density (KD) was used to identify emerging hotspot areas with potentially high concentrations of nitrogen applied to the field. Three KD classification methods were compared: defined interval (DI) into 3 classes (0-170, 170-340, > 340 kg N/ha), quantile (Q) and natural break (NB). The results show that the DI and NB areas correspond to 40 % and 84 % of the total N of buffalo origin in the Campania region, with a N surplus in the hotspot areas localized in nitrate vulnerable zones of 55 and 6 % respectively. The biogas produced from 50 % of the buffalo manure in these areas generates sufficient energy to allow the removal of the N surplus.","PeriodicalId":37987,"journal":{"name":"Acta IMEKO","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2024-03-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140378117","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-03-26DOI: 10.21014/actaimeko.v13i1.1650
R. Primi, Paolo Viola, Pedro Girotti, Pier Paolo Danieli, Bruno Ronchi, Raffaello Spina
This study seeks to evaluate a prototype's effectiveness in safeguarding livestock against wolf attacks. With an increasing imperative to protect livestock from predation, the prototype's performance was systematically examined under diverse conditions. The study primarily aimed at assessing the prototype's ability to detect wolf attacks by analyzing noise variations inherent to predator assaults. Simultaneously, the prototype aimed to mitigate livestock casualties and foster coexistence between wolves and livestock. A series of controlled experiments were meticulously carried out, replicating real-world wolf encounter scenarios. The findings yield valuable insights into the prototype's practical utility and its potential to mitigate conflicts between predator populations and livestock farming. Overall, this research contributes to advancing innovative strategies for sustainable cohabitation between wildlife and agriculture.
{"title":"Performance evaluation of a prototype for the defense against wolf attacks on livestock animals","authors":"R. Primi, Paolo Viola, Pedro Girotti, Pier Paolo Danieli, Bruno Ronchi, Raffaello Spina","doi":"10.21014/actaimeko.v13i1.1650","DOIUrl":"https://doi.org/10.21014/actaimeko.v13i1.1650","url":null,"abstract":"This study seeks to evaluate a prototype's effectiveness in safeguarding livestock against wolf attacks. With an increasing imperative to protect livestock from predation, the prototype's performance was systematically examined under diverse conditions. The study primarily aimed at assessing the prototype's ability to detect wolf attacks by analyzing noise variations inherent to predator assaults. Simultaneously, the prototype aimed to mitigate livestock casualties and foster coexistence between wolves and livestock. A series of controlled experiments were meticulously carried out, replicating real-world wolf encounter scenarios. The findings yield valuable insights into the prototype's practical utility and its potential to mitigate conflicts between predator populations and livestock farming. Overall, this research contributes to advancing innovative strategies for sustainable cohabitation between wildlife and agriculture.","PeriodicalId":37987,"journal":{"name":"Acta IMEKO","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2024-03-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140380418","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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