Biotechnology Reports最新文献_第2页

Bacteriophage-based biosensors technology: Materials, fabrications, efficiencies and shortcomings 基于噬菌体的生物传感器技术：材料、制造、效率和缺陷。

Q1 Immunology and Microbiology

Biotechnology Reports

Pub Date : 2024-12-21 DOI: 10.1016/j.btre.2024.e00872

Ihtisham Ul Haq , Kashif Rahim , Sajida Maryam , Najeeba Parre Paker

Ongoing research in biosensor technologies has led to advanced functional materials for healthcare diagnostics, and bacteriophages (phages), demonstrating exceptional utility due to their high specificity, accuracy, rapid, label-free, and wireless detection capabilities with minimal false-positive results. Phage-based-pathogen-detecting biosensors (PBPDBs) include surface plasmon resonance (SPR) biosensors, magnetoelastic (ME), electrochemical, and quartz crystal microbalance (QCM) biosensors. Commonly used substrates for PBPDBs are gold, silicon, glass, carbon-based materials, magnetic particles, and quantum dots. These substrates are chemically and physically modified to optimize phage orientation on sensor surfaces, enhancing bacterial capture. To address typical stability and issues encountered in traditional biosensor applications, phage particles and genetically modified phages are utilized to improve biosensor stability and increase detection efficacy while reducing assay time. Genetic modification in phages facilitated by CRISPR/Cas9 enables the tailoring of phages to target specific bacterial strains. This approach helps overcome the inherent specificity of phages and enables the detection of multiple pathogens in a single assay. Multiple pathogens can be detected through a single phage-based assay. This manuscript elucidates the fabrication methodologies and detection efficiencies of PBPDBs providing valuable insights into the development of practical, precise, and efficient biosensors for pathogen detection.

Summary

PBPDBs are emerging diagnostic tools for the detection of bacterial pathogens.

生物传感器技术的持续研究已经为医疗保健诊断和噬菌体（噬菌体）带来了先进的功能材料，由于其高特异性、准确性、快速、无标签和无线检测能力，以最小的假阳性结果展示了卓越的实用性。基于噬菌体的病原体检测生物传感器（PBPDBs）包括表面等离子体共振（SPR）生物传感器、磁弹性（ME）生物传感器、电化学生物传感器和石英晶体微平衡（QCM）生物传感器。pbpdb常用的衬底有金、硅、玻璃、碳基材料、磁性颗粒和量子点。这些底物经过化学和物理修饰，以优化噬菌体在传感器表面的定向，增强细菌捕获。为了解决传统生物传感器应用中遇到的典型稳定性和问题，利用噬菌体颗粒和转基因噬菌体来提高生物传感器的稳定性和提高检测效率，同时减少检测时间。CRISPR/Cas9促进了噬菌体的遗传修饰，使噬菌体能够针对特定的细菌菌株进行剪裁。这种方法有助于克服噬菌体的固有特异性，并能够在一次检测中检测多种病原体。通过一种基于噬菌体的检测方法可以检测多种病原体。本文阐述了PBPDBs的制备方法和检测效率，为开发实用、精确、高效的病原体检测生物传感器提供了宝贵的见解。摘要：PBPDBs是检测细菌性病原体的新兴诊断工具。

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引用次数: 0

Protective role of savory essential oil on vital organs in rats against deleterious effects induced by lead acetate 香薰精油对大鼠重要器官抗醋酸铅毒性作用的保护作用。

Q1 Immunology and Microbiology

Biotechnology Reports

Pub Date : 2024-12-16 DOI: 10.1016/j.btre.2024.e00871

Doha H. Aboubaker , Nermeen A. Shaffie , Mona F. Shabana , Ahmed Abd Elghafour , Bassant M.M. Ibrahim

The damaging effects of heavy metal exposure on vital organs like the heart, liver, kidneys, and brain can be lowered by natural compounds' anti-inflammatory and anti-oxidant capacity. In the current investigation, the protective potential of savory(Saturejahortensis)essential oil (EO) against lead acetate-induced multi-organ damage in rats was evaluated. Thirty female Wister Albino rats were divided into the following groups: normal, positive control given lead acetate without concomitant treatment, reference given ethylene-diamine-tetra-acetic acid and groups treated with savory EO (0.5 and 1 ml/rat), treatments were administered concomitantly with lead acetate for ten successive days. Electrocardiogram (ECG), open field, and rota-rod tests for evaluation of behaviour, as well as TNF-α, R-GSH & MDA were measured in brain and liver homogenates respectively, CRP, liver, kidney, and heart function tests, were measured in serum. Histopathology of vital organs was conducted at the end of the experiment.A high dose of savory EO, significantly improved the cardiac electrophysiology, psychological state, and locomotor activity of rats; moreover, it lowered inflammatory, oxidative biomarkers, liver, kidney, and cardiac function tests of the rats. Savory EO protects vital organs against heavy metal damage with the superiority of the high dose.

重金属对心脏、肝脏、肾脏和大脑等重要器官的破坏性影响可以通过天然化合物的抗炎和抗氧化能力来降低。本研究评估了咸味（Saturejahortensis）精油（EO）对醋酸铅诱导的大鼠多器官损伤的保护作用。选取雌性Wister Albino大鼠30只，分为正常组、阳性对照组（不同时给予醋酸铅）、参照组（不同时给予乙二胺-四乙酸）和咸味EO组（0.5、1 ml/只），连续10 d。分别在脑和肝匀浆中测量心电图（ECG）、开野试验和旋转棒试验，以及TNF-α、R-GSH和MDA，在血清中测量CRP、肝脏、肾脏和心功能试验。实验结束时进行重要脏器组织病理学检查。大剂量咸味EO显著改善大鼠心脏电生理、心理状态和运动活动；此外，它还降低了大鼠的炎症、氧化生物标志物、肝脏、肾脏和心脏功能测试。咸味EO保护重要器官免受重金属损害与高剂量的优势。

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引用次数: 0

Innovative strategies for utilizing microalgae as dual-purpose biofertilizers and phycoremediators in agroecosystems 利用微藻作为农业生态系统中双重用途生物肥料和藻修复剂的创新策略。

Q1 Immunology and Microbiology

Biotechnology Reports

Pub Date : 2024-12-10 DOI: 10.1016/j.btre.2024.e00870

Muhamad Maulana Azimatun Nur , Mahreni , Sri Wahyu Murni , Tutik Muji Setyoningrum , Faizah Hadi , Tunjung Wahyu Widayati , Danang Jaya , Raden Roro Endang Sulistyawati , Dwi Aulia Puspitaningrum , Resti Nurmala Dewi , Hadiyanto , M. Hasanuzzaman

The increasing need for sustainable agricultural practices due to the overuse of chemical fertilizers has prompted interest in microalgae as biofertilizers. This review investigates the potential of microalgae as biofertilizers and phycoremediators within sustainable agroecosystems, addressing both soil fertility and wastewater management. Microalgae provide a dual benefit by absorbing excess nutrients and contaminants from wastewater, generating nutrient-rich biomass that can replace chemical fertilizers and support plant growth. Implementation strategies include cultivating microalgae in wastewater to offset production costs, using closed photobioreactor systems to enhance growth efficiency, and applying microalgal biomass directly to soil or crops. Additionally, microalgae extracts provide essential bioactive compounds, such as phytohormones and amino acids, that enhance plant growth and resilience. While microalgae offer an eco-friendly solution for nutrient recycling and crop productivity, challenges in scalability, production cost, and regulatory frameworks hinder widespread adoption. This review highlights the potential pathways and technological advancements necessary for integrating microalgae into sustainable agriculture, emphasizing the need for interdisciplinary collaboration and innovative approaches to overcome these barriers. Ultimately, microalgae biofertilizers represent a promising approach to reducing environmental impact and advancing sustainable farming practices.

由于化肥的过度使用，对可持续农业实践的需求日益增加，这促使人们对微藻作为生物肥料产生了兴趣。本文综述了微藻在可持续农业生态系统中作为生物肥料和藻修复剂的潜力，解决了土壤肥力和废水管理问题。微藻通过吸收废水中多余的营养物质和污染物，产生富含营养的生物质，可以替代化学肥料并支持植物生长，从而提供双重效益。实施策略包括在废水中培养微藻以抵消生产成本，使用封闭光生物反应器系统提高生长效率，以及将微藻生物质直接施用于土壤或作物。此外，微藻提取物提供必需的生物活性化合物，如植物激素和氨基酸，促进植物生长和恢复力。虽然微藻为养分循环和作物生产力提供了生态友好的解决方案，但在可扩展性、生产成本和监管框架方面的挑战阻碍了微藻的广泛应用。这篇综述强调了将微藻纳入可持续农业的潜在途径和必要的技术进步，强调需要跨学科合作和创新方法来克服这些障碍。最终，微藻生物肥料代表了一种减少环境影响和促进可持续农业实践的有希望的方法。

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引用次数: 0

Prospective identification of extracellular triacylglycerol hydrolase with conserved amino acids in Amycolatopsis tolypomycina’s high G+C genomic dataset 在粘菌Amycolatopsis tolypomycina的高G+C基因组数据集中具有保守氨基酸的胞外三酰基甘油水解酶的前瞻性鉴定。

Q1 Immunology and Microbiology

Biotechnology Reports

Pub Date : 2024-12-07 DOI: 10.1016/j.btre.2024.e00869

Supajit Sraphet , Bagher Javadi

Extracellular triacylglycerol hydrolases (ETH) play a critical role for microorganisms, acting as essential tools for lipid breakdown and survival in challenging environments. The pursuit of more effective ETH genes and enzymes through evolution holds significant potential for enhancing living conditions. This study employs a proteogenomic approach to identify high G+C ETH in a notable Gram-positive bacterium, Amycolatopsis tolypomycina. Utilizing knowledge from genome and machine learning algorithms, prospective ETH genes/enzymes were identified. Notably, the ETH structural conserved accessibility to solvent clearly indicated the specific sixteen residues (GLY50, PRO93, GLY141, ASP148, GLY151, ASP172, ALA176, GLY195, TYR196, SER197, GLN198, GLY199, GLY200, GLY225, PRO327, ASP336) with no frequency. By pinpointing key residues and understanding their role, this study sets the stage for enhancing ETH performance through computational proteogenomic and contributes to the broader field of enzyme engineering, facilitating the development of more efficient and versatile ETH enzymes tailored to specific industrial or environmental contexts.

细胞外三酰基甘油水解酶（ETH）对微生物起着至关重要的作用，是在具有挑战性的环境中进行脂质分解和生存的必要工具。通过进化追求更有效的ETH基因和酶具有改善生活条件的巨大潜力。本研究采用蛋白质基因组学方法鉴定了一种显著的革兰氏阳性细菌——粘菌Amycolatopsis tolypomycina的高G+C ETH。利用基因组和机器学习算法的知识，确定了潜在的ETH基因/酶。值得注意的是，ETH对溶剂的结构保守性清晰地显示出特定的16个残基（GLY50、PRO93、GLY141、ASP148、GLY151、ASP172、ALA176、GLY195、TYR196、SER197、GLN198、GLY199、GLY200、GLY225、PRO327、ASP336）无频率。通过精确定位关键残基并了解其作用，本研究为通过计算蛋白质基因组学提高ETH性能奠定了基础，并为酶工程的更广泛领域做出了贡献，促进了针对特定工业或环境背景的更高效、更通用的ETH酶的开发。

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引用次数: 0

Light intensity effects on bioproduct recovery from fuel synthesis wastewater using purple phototrophic bacteria in a hybrid biofilm-suspended growth system 光强对混合生物膜悬浮生长系统中紫色光养细菌从燃料合成废水中回收生物产品的影响

Q1 Immunology and Microbiology

Biotechnology Reports

Pub Date : 2024-12-01 DOI: 10.1016/j.btre.2024.e00863

Sultan Shaikh , Gordon McKay , Hamish Robert Mackey

This research looked at how three different light intensities (1600, 4300, and 7200 lx) affect the biomass development, treatment of fuel synthesis wastewater and the recovery of valuable bioproducts between biofilm and suspended growth in a purple-bacteria enriched photobioreactor. Each condition was run in duplicate using an agricultural shade cloth as the biofilm support media in a continuously mixed batch reactor. The results showed that the highest chemical oxygen demand (COD) removal rate (56.8 ± 0.9 %) was found under the highest light intensity (7200 lx), which also led to the most biofilm formation and highest biofilm biomass production (1225 ± 95.7 mg). The maximum carotenoids (Crts) and bacteriochlorophylls (BChls) content occurred in the suspended growth of the 7200 lx reactor. BChls decreased with light intensity in suspended growth, while in biofilm both Crts and BChls were relatively stable between light conditions, likely due to an averaging effect as biofilm thickened at higher light intensity. Light intensity did not affect protein content of the biomass, however, biofilm showed a lower average (41.2 % to 43.7 %) than suspended biomass (45.4 % to 47.7 %). For polyhydroxybutyrate (PHB) the highest cell concentration in biofilm occurred at 1600 lx (11.4 ± 2.4 %), while for suspended growth it occurred at 7200 lx (22.7 ± 0.3 %), though total PHB productivity remained similar between reactors. Shading effects from the externally located biofilm could explain most variations in bioproduct distribution. Overall, these findings suggest that controlling light intensity can effectively influence the treatment of fuel synthesis wastewater and the recovery of valuable bioproducts in a biofilm photobioreactor.

本研究研究了三种不同的光强度（1600、4300和7200 lx）如何影响紫色细菌富集光生物反应器中生物质的发展、燃料合成废水的处理以及生物膜和悬浮生长之间有价值的生物产品的回收。采用农用遮光布作为生物膜支撑介质，在连续混合间歇式反应器中进行两种条件的试验。结果表明，在最高光照强度（7200 lx）下，化学需氧量（COD）去除率最高（56.8%±0.9%），生物膜生成量最高（1225±95.7 mg）。类胡萝卜素（Crts）和细菌叶绿素（BChls）含量在7200lx反应器悬浮生长时最高。在悬浮生长中，BChls随光强而下降，而在生物膜中，Crts和BChls在不同光照条件下相对稳定，可能是由于生物膜在较高光强下增厚的平均效应。光照强度对生物量蛋白质含量没有影响，但生物膜的平均蛋白质含量（41.2% ~ 43.7%）低于悬浮生物量（45.4% ~ 47.7%）。对于聚羟基丁酸盐（PHB），生物膜中的最高细胞浓度发生在1600 lx(11.4±2.4%)，而悬浮生长的最高细胞浓度发生在7200 lx(22.7±0.3%)，尽管反应器之间的PHB总产量相似。外部生物膜的遮阳效应可以解释生物制品分布的大多数变化。总之，这些研究结果表明，控制光强度可以有效地影响生物膜光生物反应器中燃料合成废水的处理和有价值生物产品的回收。

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引用次数: 0

Assessment of microbial antagonistic activity and Quorum Sensing Signal Molecule (Cyclopeptides-DKPs and N-Acyl Homoserine Lactones) detection in bacterial strains obtained from avocado thrips (Thysanoptera: Thripidae) 牛油果蓟马微生物拮抗活性评价及群体感应信号分子（环肽- dkps和n -酰基同型丝氨酸内酯）检测

Q1 Immunology and Microbiology

Biotechnology Reports

Pub Date : 2024-11-29 DOI: 10.1016/j.btre.2024.e00866

A.N. Pereira-Bazurdo , G.E. Cadavid-Restrepo , R.E. Arango-Isaza , C.X. Moreno-Herrera

The control of avocado pests and diseases heavily relies on the use of several types of pesticides, some of which are strictly monitored or not internationally accepted. New sources of bioactive molecules produced by phytopathogen-inhibiting microorganisms offer an excellent alternative for the control of pests and diseases. This study explores the potential antagonistic action against phytopathogenic microorganisms, using bacterial strains obtained from avocado thrips. In addition, we detected and identified quorum sensing (QS) signaling molecules that are related to virulence factors and antibiotic production. The results showed that Bacillus, Pantoea, and Serratia strains exhibited antagonism against five fungal phytopathogens. Additionally, some bacteria also produce specific signaling molecules like N-3-(oxododecanoyl)-l-homoserine lactone (OdDHL), N-(3-oxo)-hexanoyl l-HL (OHHL), 4‑hydroxy-2-heptylquinoline (HHQ) or 2-heptyl-3,4-dihydroxyquinoline (PQS, Pseudomonas quinolone signal), cyclo(L-Phe-l-Pro), and cyclo(L-Pro-l-Tyr, which might give them antimicrobial properties. This research explores the biotechnological potential of these bacteria in fighting the diseases affecting avocados in Colombia.

牛油果病虫害的控制严重依赖于几种农药的使用，其中一些农药受到严格监控或不被国际认可。植物病原菌抑制微生物产生的生物活性分子的新来源为防治病虫害提供了一个很好的选择。本研究利用从鳄梨蓟马中获得的细菌菌株，探讨了对植物病原微生物的潜在拮抗作用。此外，我们检测并鉴定了与毒力因子和抗生素产生相关的群体感应（QS）信号分子。结果表明，芽孢杆菌、Pantoea和Serratia菌株对5种真菌植物病原体具有拮抗作用。此外，一些细菌还产生特定的信号分子，如N-3-（氧十二烷基）-l-丝氨酸内酯（OdDHL）、N-（3-氧）-己醇基l-HL （OHHL）、4-羟基-2-庚基喹啉（HHQ）或2-庚基-3,4-二羟基喹啉（PQS，喹诺酮假单胞菌信号）、环（l- ph -l- pro）和环（L-Pro-l-Tyr），这些信号分子可能赋予它们抗菌特性。这项研究探索了这些细菌在对抗影响哥伦比亚鳄梨的疾病方面的生物技术潜力。

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引用次数: 0

Design of Natterins-based peptides improves antimicrobial and antiviral activities 基于纳豆素的多肽的设计提高了抗菌和抗病毒活性。

Q1 Immunology and Microbiology

Biotechnology Reports

Pub Date : 2024-11-28 DOI: 10.1016/j.btre.2024.e00867

Gabrielle L. de Cena , Dayane B. Tada , Danilo B.M. Lucchi , Tiago A.A. Santos , Montserrat Heras , Maria Juliano , Carla Torres Braconi , Miguel A.R.B. Castanho , Mônica Lopes-Ferreira , Katia Conceição

The biochemical analysis of animal venoms has been intensifying over the years, enabling the prediction of new molecules derived from toxins, harnessing the therapeutic potential of these molecules. From the venom of the fish Thalassophryne nattereri, using in silico methods for predicting antimicrobial and cell-penetrating peptides, two peptides from Natterins with promising characteristics were synthesized and subjected to in vitro and in vivo analysis. The peptides were subjected to stability tests and antimicrobial assays, cytotoxicity in murine fibroblast cells, antiviral assays against the Chikungunya virus, and the toxicity on G. mellonella was also evaluated. The findings underscore the peptides' robust stability under varying temperatures and pH conditions and resistance to proteolytic degradation. The peptides demonstrated significant antimicrobial efficacy, minimal cytotoxicity, and low hemolytic activity. Although their antiviral efficacy was limited, they showed potential at specific stages of viral replication. The in vivo toxicity tests indicated a favorable safety profile. These findings suggest that this approach can aid in the development of antimicrobial agents, offering a faster and personalized method to combat microbial infections, and represent a promising discovery in venom biotechnology research.

多年来，动物毒液的生化分析一直在加强，能够预测来自毒素的新分子，利用这些分子的治疗潜力。从海苔鱼（Thalassophryne nattereri）的毒液中，利用计算机预测抗菌肽和穿透细胞肽的方法，合成了两种具有良好特性的海苔蛋白肽，并进行了体外和体内分析。对制备的多肽进行了稳定性试验、抗菌试验、小鼠成纤维细胞毒性试验、基孔肯雅病毒抗病毒试验和对大麦伦氏杆菌的毒性试验。这一发现强调了肽在不同温度和pH条件下的强大稳定性以及对蛋白质水解降解的抵抗力。这些肽显示出显著的抗菌功效、最小的细胞毒性和低溶血活性。尽管它们的抗病毒功效有限，但它们在病毒复制的特定阶段显示出潜力。体内毒性试验表明具有良好的安全性。这些发现表明，这种方法可以帮助开发抗菌药物，提供一种更快和个性化的方法来对抗微生物感染，并代表了毒液生物技术研究的一个有希望的发现。

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引用次数: 0

Engineering Nicotiana benthamiana for production of active cannabinoid synthases via secretory pathway optimization 通过分泌途径优化，工程烟叶生产活性大麻素合成酶

Q1 Immunology and Microbiology

Biotechnology Reports

Pub Date : 2024-11-27 DOI: 10.1016/j.btre.2024.e00865

Omayra C. Bolaños-Martínez , Anna Urbanetz , Daniel Maresch , Richard Strasser , Sornkanok Vimolmangkang

The production of cannabinoid compounds such as Δ9-tetrahydrocannabinol (THC), cannabidiol (CBD) and cannabichromene (CBC) with potential pharmaceutical applications is growing sharply. However, challenges such as the low yield of minor cannabinoids, legal restrictions on cultivation, and the complexity and cost of purification from the Cannabis sativa plant necessitate a biotechnological approach. Since the biosynthetic pathway is disclosed, cannabinoids have been produced in yeast, insect cells and plants mainly by the heterologous expression of tetrahydrocannabinol acid synthase (THCAS). THCAS and cannabidiolic acid synthase (CBDAS) use cannabigerolic acid (CBGA) as a substrate. In this study, we transiently expressed recombinant forms of THCAS and CBDAS in leaves of Nicotiana benthamiana. Our results demonstrate that efficient expression in the secretory pathway relies on replacing the endogenous signal peptide with a heterologous one. Both proteins were successfully secreted to the apoplast. MS-based analysis of the purified proteins revealed that they are heavily glycosylated with mainly Golgi-processed complex type N-glycans. In planta enzymatic removal of N-glycans indicated that glycosylation plays a role for CBDAS protein folding or stability. Finally, in vitro assays with CBGA showed that the plant-made recombinant CBDAS and THCAS are enzymatically active.

具有潜在制药应用价值的大麻素化合物如Δ9-tetrahydrocannabinol （THC）、大麻二酚（CBD）和大麻红素（CBC）的生产正在急剧增长。然而，诸如少量大麻素产量低、对种植的法律限制以及从大麻植物中提纯的复杂性和成本等挑战需要采用生物技术方法。自该生物合成途径被披露以来，大麻素主要通过四氢大麻酚酸合成酶（THCAS）的异源表达在酵母、昆虫细胞和植物中产生。THCAS和大麻二酚酸合成酶（CBDAS）使用大麻二酚酸（CBGA）作为底物。在本研究中，我们在烟叶中瞬时表达了THCAS和CBDAS的重组形式。我们的研究结果表明，分泌途径的有效表达依赖于用外源信号肽替代内源信号肽。两种蛋白都成功地分泌到外质体中。纯化蛋白的质谱分析显示，它们主要被高尔基加工的n -聚糖严重糖基化。在植物中n -聚糖的酶解表明糖基化对CBDAS蛋白的折叠或稳定性起作用。最后，体外CBGA实验表明，植物合成的重组CBDAS和THCAS具有酶活性。

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引用次数: 0

Immobilizing calcium-dependent affinity ligand onto iron oxide nanoparticles for mild magnetic mAb separation 将钙依赖的亲和配体固定在氧化铁纳米颗粒上用于弱磁单抗分离

Q1 Immunology and Microbiology

Biotechnology Reports

Pub Date : 2024-11-26 DOI: 10.1016/j.btre.2024.e00864

Ines Zimmermann , Friederike Eilts , Anna-Sophia Galler , Jonas Bayer , Sophia Hober , Sonja Berensmeier

Current downstream processing of monoclonal antibodies (mAbs) is limited in throughput and requires harsh pH conditions for mAb elution from Protein A affinity ligands. The use of an engineered calcium-dependent ligand (Z_Ca) in magnetic separation applications promises improvements due to mild elution conditions, fast processability, and process integration prospects. In this work, we synthesized and evaluated three magnetic nanoparticle types immobilized with the cysteine-tagged ligand Z_Ca-cys. Ligand homodimers were physically immobilized onto bare iron oxide nanoparticles (MNP) and MNP coated with tetraethyl orthosilicate (MNP@TEOS). In contrast, Z_Ca-cys was covalently and more site-directedly immobilized onto MNP coated with (3-glycidyloxypropyl)trimethoxysilane (MNP@GPTMS) via a preferential cysteine-mediated epoxy ring opening reaction. Both coated MNP showed suitable characteristics, with MNP@TEOS@Z_Ca-cys demonstrating larger immunoglobulin G (IgG) capacity (196 mg g⁻¹) and the GPTMS-coated particles showing faster magnetic attraction and higher IgG recovery (88 %). The particles pave the way for the development of calcium-dependent magnetic separation processes.

目前单克隆抗体（mAb）的下游加工在吞吐量上是有限的，并且需要苛刻的pH条件才能从Protein A亲和配体中洗脱mAb。在磁分离应用中使用工程钙依赖配体（ZCa），由于温和的洗脱条件，快速的可加工性和过程集成的前景，有望改善。在这项工作中，我们合成并评价了三种用半胱氨酸标记的配体ZCa-cys固定的磁性纳米颗粒类型。将配体同二聚体物理固定在裸氧化铁纳米颗粒（MNP）和正硅酸四乙酯包被的MNP上（MNP@TEOS）。相比之下，ZCa-cys通过半胱氨酸介导的环氧开环反应，共价且更直接地固定在（3-甘油基氧基丙基）三甲氧基硅烷（MNP@GPTMS）包被的MNP上。两种包被的MNP均表现出合适的特性，MNP@TEOS@ZCa-cys具有更大的免疫球蛋白G （IgG）容量（196 mg G - 1）， gptms包被的MNP具有更快的磁性吸引和更高的IgG回收率（88%）。这些粒子为依赖钙的磁分离工艺的发展铺平了道路。

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引用次数: 0

Bee products: An overview of sources, biological activities and advanced approaches used in apitherapy application 蜜蜂产品：蜂产品来源、生物活性和蜂疗应用先进方法概述

Q1 Immunology and Microbiology

Biotechnology Reports

Pub Date : 2024-10-18 DOI: 10.1016/j.btre.2024.e00862

Samia E. El-Didamony , Hend I.A. Gouda , Mahmoud M.M. Zidan , Reham I. Amer

Background

Bee therapy (Apitherapy, Api-treatment, Bee treatment) is a type of biotherapy that uses bees and their products as medicinal or preventative measures to control progression of diseases. In many countries today, apitherapy is a section of complementary and integrative medicine. The aim of this review is to explore the different bee products and their therapeutic potentials.

Method

We searched the literature and then explored and evaluated evidence for bee products’ composition, therapeutic abilities and novel techniques used to enhance their effectiveness.

Results

Data revealed that there are continuous advances in research and clinical trials of bee therapy. A better understanding of the composition of bee products generated great interest in their use for medical treatments. Bee products either collected or synthesized promote healing through reducing inflammation, enhancing circulation, and inducing a healthy immunological response, Furthermore, researchers have developed innovative approaches such as nanoparticles, scaffold, nanofibers, and others to increase the bioavailability of bee products and overcome problems with the traditional use of these products.

Conclusion

Bee therapy is a simple, accessible, and easy-to-use pharmaceutical that is used in conventional medicine and has the potential to treat a variety of diseases. However, further studies are needed to prove its efficacy, and safety. Lack of practice regulations is still an issue.

背景蜜蜂疗法（Apitherapy、Api-treatment、Bee treatment）是一种生物疗法，利用蜜蜂及其产品作为药物或预防措施来控制疾病的发展。在当今许多国家，蜂疗是补充医学和综合医学的一部分。本综述旨在探讨不同的蜂产品及其治疗潜力。方法我们检索了相关文献，然后对蜂产品的成分、治疗能力和用于提高其疗效的新技术进行了探讨和评估。结果数据显示，蜂疗的研究和临床试验不断取得进展。数据显示，蜂疗研究和临床试验不断取得进展，对蜂产品成分的深入了解使人们对蜂产品的医疗用途产生了浓厚的兴趣。此外，研究人员还开发了创新方法，如纳米颗粒、支架、纳米纤维等，以提高蜂产品的生物利用度，并克服这些产品的传统使用问题。然而，还需要进一步的研究来证明其有效性和安全性。缺乏实践规范仍是一个问题。

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