Biotechnology Reports最新文献_第3页

Improving a photosynthetic bioprocess with a ubiquitous additive: Using clay powder in the cultivation of Rhodopseudomonas palustris 用普遍存在的添加剂改善光合生物过程：粘土粉在古红假单胞菌培养中的应用

Q1 Immunology and Microbiology

Biotechnology Reports

Pub Date : 2025-10-08 DOI: 10.1016/j.btre.2025.e00930

Sheida Stephens , Mona Abo-Hashesh , Radhakrishnan Mahadevan , D. Grant Allen

A key challenge in process optimization is reactor performance, particularly in light-dependent bioprocesses. While novel photobioreactor designs exist, adapting established bioreactors with simple media supplementation may provide a more practical and modular alternative to complex mechanical modifications. In this study, we evaluated the effect of supplementing 0.2 % (w/v) clay powder on acetate production by Rhodopseudomonas palustris grown on butyrate. Among the clay types tested, kaolin showed the most pronounced benefits, with acetate accumulation increasing by 45 % relative to controls. These improvements are attributed to a combination of enhanced light distribution and increased cellular aggregation. Photosynthetic photon flux density measurements confirmed that kaolin reduced the extent of cellular shading by increasing light scattering within the culture. Taken together, these findings demonstrate that supplementation with a widely available clay can improve light penetration in suspended phototrophic cultures, offering a novel andaccessible strategy for optimizing photosynthetic bioprocesses.

工艺优化的一个关键挑战是反应器性能，特别是在依赖光的生物过程中。虽然存在新的光生物反应器设计，但通过简单的培养基补充来适应已建立的生物反应器可能为复杂的机械修改提供更实用和模块化的替代方案。本试验研究了添加0.2% （w/v）粘土粉对生长在丁酸盐上的古红假单胞菌产乙酸的影响。在测试的粘土类型中，高岭土表现出最显著的效益，乙酸积累量相对于对照增加了45%。这些改进是由于增强的光分布和增加的细胞聚集的结合。光合光子通量密度测量证实，高岭土通过增加培养物内的光散射减少了细胞遮阳的程度。综上所述，这些发现表明，添加广泛可用的粘土可以提高悬浮光养培养物的透光性，为优化光合生物过程提供了一种新颖而可行的策略。

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引用次数: 0

Sustainable fuel and power from biomass: 4E analysis of a solar-assisted DME production system with CO₂ capture 来自生物质的可持续燃料和动力：具有二氧化碳捕获的太阳能辅助二甲醚生产系统的4E分析

Q1 Immunology and Microbiology

Biotechnology Reports

Pub Date : 2025-10-02 DOI: 10.1016/j.btre.2025.e00928

Atieh Kermani , Farzin Hosseinifard , Mohsen Salimi , Majid Amidpour

Transitioning to clean energy requires efficient, low-carbon fuel production methods. Traditional biomass-to-fuel approaches are limited by inefficiency, cost, and emissions. This study presents an innovative system based on renewable lignocellulosic biomass to produce dimethyl ether (DME), methanol, and electricity simultaneously. The design integrates thermochemical conversion, solar thermal energy, internal power generation, and post-combustion CO₂ capture. Waste heat and solar energy drive dual electricity-producing loops, enabling internal energy sufficiency and surplus sale. Simulations indicate 50 % total energy efficiency and 49 % exergy efficiency, with hourly production of 2.7 tons DME and 0.56 tons methanol. Economic analysis shows baseline feasibility with an NPV of ∼$530 M and payback period ∼5.5 years; sensitivity to biomass price, capital cost, and discount rate is noted, highlighting potential uncertainty ranges. This integrated pathway offers a scalable, low-carbon, and economically viable solution for sustainable bioenergy.

向清洁能源过渡需要高效、低碳的燃料生产方法。传统的生物质转化为燃料的方法受到低效率、成本和排放的限制。本研究提出了一种基于可再生木质纤维素生物质的创新系统，可以同时生产二甲醚（DME）、甲醇和电力。该设计集成了热化学转换、太阳能热能、内部发电和燃烧后二氧化碳捕获。废热和太阳能驱动双重发电循环，实现内部能源自给和剩余销售。模拟表明，总能源效率为50%，火用效率为49%，每小时生产2.7吨二甲醚和0.56吨甲醇。经济分析显示，基线可行性NPV为5.3亿美元，投资回收期为5.5年；注意到对生物质价格、资本成本和贴现率的敏感性，突出了潜在的不确定性范围。这种综合途径为可持续生物能源提供了一种可扩展的、低碳的、经济上可行的解决方案。

引用次数: 0

Glycoengineering of plant-produced Pembrolizumab enhances FcRn binding and extends serum half-life in mice 糖工程植物产生的Pembrolizumab增强FcRn结合并延长小鼠血清半衰期

Q1 Immunology and Microbiology

Biotechnology Reports

Pub Date : 2025-09-29 DOI: 10.1016/j.btre.2025.e00927

Christine Joy I. Bulaon , Janejira Jaratsittisin , Kaewta Rattanapisit , Pipob Suwanchaikasem , Shiying Guo , Khwanchit Boonha , Pannamthip Pitaksajjakul , Nipaporn Simsom , Vudhiporn Limprasutr , Waranyoo Phoolcharoen

Plant systems offer scalable and cost-effective platforms for antibody production, but plant-specific glycans may affect pharmacokinetics and immunogenicity. To evaluate the impact of Fc glycosylation, four Pembrolizumab glycovariants were generated in Nicotiana benthamiana: wild-type glycosylation (Pembro-WT), high-mannose with SEKDEL (Pembro-KD), aglycosylated N297A mutant (Pembro-NG), and a core fucose/xylose-deficient variant (Pembro-XF). Glycoproteins were transiently expressed either in wild-type or ΔXF plants, purified, and characterized for glycan composition, in vitro binding, and in vivo pharmacokinetics. LC-MS confirmed distinct glycoform patterns, while PD-1 binding was retained across all variants. Pembro-XF showed the highest FcRn binding affinity and longest serum half-life (45.83 h) in mice, compared to Pembro-WT (26.7 h), Pembro-KD (32.95 h), Pembro-NG (34.27 h), and Keytruda® (33.26 h). As an initial efficacy evaluation, Pembro-WT demonstrated strong antitumor activity in a murine colon cancer model. These findings support plant glycoengineering as a strategy to enhance antibody pharmacokinetics and advance next generation antibody therapeutics.

植物系统为抗体生产提供了可扩展且具有成本效益的平台，但植物特异性聚糖可能会影响药代动力学和免疫原性。为了评估Fc糖基化的影响，在benthamiana中产生了四种Pembrolizumab糖变体：野生型糖基化（pembrowt），高甘露糖与SEKDEL (pembrokd)，糖基化N297A突变体（pembrong）和核心焦点/木糖缺乏变体（pembroxf）。糖蛋白在野生型或ΔXF植物中短暂表达，经过纯化，并被表征为聚糖组成、体外结合和体内药代动力学。LC-MS证实了不同的糖型模式，而PD-1结合在所有变体中都保留。与pembrom - wt （26.7 h）、pembrom - kd （32.95 h）、pembrom - ng （34.27 h）和Keytruda®（33.26 h）相比，pembrom - xf在小鼠中表现出最高的FcRn结合亲和力和最长的血清半衰期（45.83 h）。作为初步疗效评估，pemm - wt在小鼠结肠癌模型中显示出较强的抗肿瘤活性。这些发现支持植物糖工程作为增强抗体药代动力学和推进下一代抗体治疗的策略。

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引用次数: 0

Discovery and engineering of bifunctional enzymes for lignocellulose degradation: Metagenomic and computational approaches 木质纤维素降解双功能酶的发现和工程：宏基因组和计算方法

Q1 Immunology and Microbiology

Biotechnology Reports

Pub Date : 2025-09-20 DOI: 10.1016/j.btre.2025.e00926

Razieh Goudarzi , Donya Afshar Jahanshahi , Arashk Kavousi , Shohreh Ariaeenejad

Efficient degradation of lignocellulosic biomass is vital for converting plant-based waste into renewable fuels and chemicals. Owing to its complex composition of cellulose, hemicellulose, and lignin, its enzymatic breakdown often requires multiple enzymes to act synergistically. Bifunctional enzymes that combine two catalytic activities in a single protein offer a promising solution. This review highlights recent advances in the identification and engineering of bifunctional enzymes for lignocellulose degradation, particularly through metagenomics, protein fusion and computational design. Functional pairings, such as cellulase/xylanase, were examined with a focus on their synergistic effects, substrate specificity, and stability. Promiscuous and naturally evolved bifunctional enzymes from extreme or uncultured environments are also discussed. Advances in silico modeling and directed evolution have enhanced enzyme properties such as thermostability and substrate range. The review concludes with an outlook on the challenges and opportunities of implementing bifunctional enzymes to improve the economic and technical viability of biomass conversion.

木质纤维素生物质的有效降解对于将植物废料转化为可再生燃料和化学品至关重要。由于其纤维素、半纤维素和木质素的复杂组成，它的酶分解通常需要多种酶协同作用。将两种催化活性结合在一个蛋白质中的双功能酶提供了一个有希望的解决方案。本文综述了近年来在木质纤维素降解双功能酶的鉴定和工程方面的进展，特别是在宏基因组学、蛋白质融合和计算设计方面的进展。功能配对，如纤维素酶/木聚糖酶，研究重点是它们的协同作用，底物特异性和稳定性。还讨论了从极端或非培养环境中混杂和自然进化的双功能酶。硅建模和定向进化的进步提高了酶的性能，如热稳定性和底物范围。本文最后展望了利用双功能酶提高生物质转化的经济和技术可行性所面临的挑战和机遇。

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引用次数: 0

Numerical modelling and experimental validation of an orifice plate-based hydrodynamic cavitation process for improving the biomass pretreatment 基于孔板的水力空化工艺改进生物质预处理的数值模拟与实验验证

Q1 Immunology and Microbiology

Biotechnology Reports

Pub Date : 2025-09-12 DOI: 10.1016/j.btre.2025.e00925

Shetal Roy , Souman Rudra , DI Muhandiram , MM Roshid

Hydrodynamic cavitation can be used to pretreat biomass by utilizing the energy released during the collapse of cavitation bubbles. Delignification as pretreatment enhances the biodegradation of lignocellulosic composition. The present study employed a CFD model and experimental validation of the numerical analysis of hydrodynamic cavitation (HC) in multi-hole orifice (MHO) designs under various operating conditions. The total 14 geometry of the orifice plate depends on the plate thickness, the number of holes, and the hole orientation used to analyze and optimize geometry for the biomass pretreatment process. Three phases are used in numerical analysis and experimentation to investigate the effect of particles on cavitation. The simulated results regarding velocity and pressure gradients, turbulence quantities, and vapor volume fractions are critically analyzed and discussed. The cavitation number changes to 0.22 in the experiment and 0.16 in the simulation due to the presence of biomass particles. Orifice plate thickness was found to significantly influence cavitation inception and evolution. 4 mm thickness and nine holes with specific orientations were found to be an optimized geometry with the lowest cavitation number, maximum pressure drop, and highest throat velocity. In the experimentation, 0.8 mm biomass particles were used in the mixture (2 % w/w) to determine the effect of biomass particles on the flow. This result helps identify the critical operating and design parameters and the impact on the cavitation of particles to achieve the desired cavitation phenomena.

水动力空化可以利用空化气泡破裂时释放的能量对生物质进行预处理。脱木质素预处理提高了木质纤维素成分的生物降解。本文采用CFD模型和实验验证方法，对不同工况下多孔口设计中的水动力空化现象进行了数值分析。孔板的总几何形状取决于板的厚度、孔的数量以及用于分析和优化生物质预处理过程的孔的方向。采用三相数值分析和实验方法研究了颗粒对空化的影响。对速度和压力梯度、湍流量和蒸汽体积分数的模拟结果进行了严格的分析和讨论。由于生物质颗粒的存在，实验空化数为0.22，模拟空化数为0.16。孔板厚度对空化的产生和演化有显著影响。结果表明，厚度为4mm、孔径为9个定向孔的优化几何形状具有最小的空化数、最大的压降和最高的喉道速度。实验中，在混合物中加入0.8 mm的生物质颗粒（2% w/w），以确定生物质颗粒对流动的影响。该结果有助于确定关键的操作和设计参数以及对颗粒空化的影响，以实现所需的空化现象。

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引用次数: 0

Biodecolorization and Biodegradation of Methyl Orange by Immobilized Pseudomonas aeruginosa Bacterium into SA/PVA Matrix Integrated with MOF UiO-66 Adsorbent 固定化铜绿假单胞菌对SA/PVA复合MOF UiO-66吸附剂对甲基橙的生物脱色降解

Q1 Immunology and Microbiology

Biotechnology Reports

Pub Date : 2025-09-05 DOI: 10.1016/j.btre.2025.e00924

Sarazen Shalahuddin Akbar, Adi Setyo Purnomo , Silvia Abdi Pratama, Berlian Imazdalifa, Sifra Thrivelia Krisnia Ayu, Reyza Fadly Maghfiroh, Nur Annisa Kusumawardhani, Ardi Lukman Hakim

Azo dye, such as methyl orange (MO), is characterized by environmental concerns due to persistence, toxicity, and potential carcinogenic effects. Therefore, this study aimed to investigate the integration of immobilized Pseudomonas aeruginosa (PA) and UiO-66, a zirconium-based metal-organic framework (MOF), into sodium alginate/polyvinyl alcohol (SA/PVA) matrix for biodecolorization and biodegradation of MO dye. Material characterization was carried out by using X-ray diffraction (XRD), Fourier-transform infrared (FTIR), and scanning electron microscopy-energy dispersive X-ray (SEM-EDX) which showed the successful formation of UiO-66 and stable incorporation in SA/PVA/PA beads. The results of adsorption experiments showed that the presence of UiO-66 enhanced MO adsorption capacity by 20.47 % compared to SA/PVA/PA matrix without UiO-66. Kinetic analysis followed the pseudo-second-order model, and adsorption isotherm was best described by the Langmuir model, showing monolayer adsorption behavior. Furthermore, biodecolorization results showed that SA/PVA/UiO-66/PA beads achieved a 92.03 % MO removal rate, indicating a 33.99 % higher than free PA cells. Liquid chromatography–mass spectrometry (LC-MS) analysis confirmed the formation of nine biodegradation products, suggesting successful structural breakdown of MO. These results showed that combining UiO-66 with immobilized PA significantly enhanced both adsorption and biodegradation performance toward MO treatment.

偶氮染料，如甲基橙（MO），因其持久性、毒性和潜在的致癌作用而受到环境问题的关注。因此，本研究旨在研究将固定化铜绿假单胞菌（PA）和锆基金属有机骨架（MOF） UiO-66整合到海藻酸钠/聚乙烯醇（SA/PVA）基质中，用于MO染料的生物脱色和生物降解。通过x射线衍射（XRD）、傅里叶变换红外（FTIR）和扫描电镜-能量色散x射线（SEM-EDX）对材料进行了表征，结果表明UiO-66成功形成，并稳定地结合在SA/PVA/PA微珠中。吸附实验结果表明，与不含UiO-66的SA/PVA/PA基质相比，UiO-66的存在使MO吸附量提高了20.47%。动力学分析采用拟二阶模型，Langmuir模型最能描述吸附等温线，呈现单层吸附行为。此外，生物脱色结果表明，SA/PVA/UiO-66/PA微球对MO的去除率达到92.03%，比游离PA细胞高出33.99%。液相色谱-质谱（LC-MS）分析证实了9种生物降解产物的形成，表明MO的结构被成功分解。这些结果表明，UiO-66与固定化PA的结合显著提高了对MO的吸附和生物降解性能。

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引用次数: 0

Solving the light chain mismatch of IgG-like bispecific antibody by utilizing 2A peptide based FaBody platform 利用基于2A肽的FaBody平台解决igg样双特异性抗体轻链错配问题

Q1 Immunology and Microbiology

Biotechnology Reports

Pub Date : 2025-08-30 DOI: 10.1016/j.btre.2025.e00917

Dong Li , Pengfan Zheng , Xuejing Yao , Baopeng Zhang , Jiekun Zhang , Yaocheng Qu , Shasha Yun , Yanzhen Li , Shanshan Chen , Jianmin Fang

Bispecific antibodies (BsAbs), engineered to target multiple antigens or epitopes simultaneously, promise enhanced therapeutic efficacy over traditional monoclonal antibodies (mAbs). However, the complex BsAbs structure presents significant production challenges, particularly chain mismatch issues. This study presents a novel approach utilizing 2A peptides within a FaBody platform to address light chain mismatches in IgG-like BsAbs. By leveraging self-cleaving 2A peptides, stable expression in mammalian cells significantly improves the accuracy of antibody chain assembly. This strategy markedly enhances the production of correctly assembled IgG-like BsAbs, providing a promising solution to critical challenges in BsAbs drug development.

双特异性抗体（BsAbs）可以同时靶向多种抗原或表位，有望比传统的单克隆抗体（mab）具有更好的治疗效果。然而，复杂的bsab结构带来了重大的生产挑战，特别是链不匹配问题。本研究提出了一种利用FaBody平台中的2A肽来解决igg样bsab轻链错配的新方法。通过利用自切割2A肽，在哺乳动物细胞中的稳定表达显著提高了抗体链组装的准确性。这一策略显著提高了正确组装igg样bsab的生产，为bsab药物开发中的关键挑战提供了一个有希望的解决方案。

引用次数: 0

Plant growth-promoting traits of Rhizophora mangle-associated bacteria and their effects on tomato (Solanum lycopersicum) 根霉相关菌的植物促生特性及其对番茄的影响

Q1 Immunology and Microbiology

Biotechnology Reports

Pub Date : 2025-08-27 DOI: 10.1016/j.btre.2025.e00923

Lucumi-Banguero Ruby Stella, Bustos-Barona María Alejandra, López-Velázquez Ximena Andrea, Aguirre-Ramírez Elkin, Benítez-Campo Neyla

Mangrove ecosystems harbor a remarkable microbial diversity that plays essential roles in nutrient recycling and transformation, contributing to soil fertility and quality. However, the exploration of this microbiota as a source of plant growth-promoting bacteria (PGPB) remains at an early stage. In this study, bacterial strains associated with the rhizosphere of Rhizophora mangle from Colombian Pacific mangroves were characterized and their effects on Solanum lycopersicum var. cerasiforme were evaluated. Strains were isolated using nitrogen-free media and identified through 16S rRNA gene sequencing. Key plant growth-promoting (PGP) functions were assessed, including biological nitrogen fixation (confirmed by the presence of the nifH gene), indole-3-acetic acid (IAA) production, siderophore synthesis, and phosphate and potassium solubilization. All isolates fixed nitrogen and produced IAA; 50 % synthesized siderophores, 72 % solubilized potassium, and 90.9 % solubilized phosphate. A factorial analysis of mixed data (FAMD) was used to select four functionally promising strains: Raoultella terrigena RFN53, Klebsiella variicola RFN55, Cellulosimicrobium sp. RFN73, and Klebsiella sp. RFN75, which were evaluated in independent paired greenhouse trials. Inoculation with Cellulosimicrobium sp. RFN73, as well as the combinations RFN53+RFN55 and RFN73+RFN75, significantly enhanced plant growth, increasing plant height, leaf area, and biomass accumulation (p < 0.05) compared to the control. These findings highlight the potential of R. mangle-associated bacteria as biofertilizers and underscore the importance of including safety assessments and detailed functional characterization to ensure their responsible use in sustainable agricultural systems.

红树林生态系统拥有显著的微生物多样性，在养分循环和转化中发挥重要作用，有助于土壤肥力和质量。然而，这种微生物群作为植物生长促进菌（PGPB）来源的探索仍处于早期阶段。本研究对哥伦比亚太平洋红树根霉（Rhizophora mangle）根际相关菌种进行了鉴定，并对其对番茄茄（Solanum lycopersicum变种）的影响进行了评价。采用无氮培养基分离菌株，通过16S rRNA基因测序进行鉴定。研究人员评估了植物促生长（PGP）的关键功能，包括生物固氮（由nifH基因的存在证实）、吲哚-3-乙酸（IAA）的产生、铁载体的合成以及磷酸盐和钾的增溶。所有分离株均固定氮并产生IAA；50%合成了铁载体，72%溶解了钾，90.9%溶解了磷酸盐。采用混合数据的因子分析（FAMD）筛选了4株功能较好的菌株：terrigena Raoultella RFN53、variicola Klebsiella RFN55、cellulosimicroum sp. RFN73和Klebsiella sp. RFN75，并在独立的温室配对试验中进行了评价。接种纤维素微生物sp. RFN73，以及RFN53+RFN55和RFN73+RFN75组合，与对照相比，显著促进了植株生长，提高了株高、叶面积和生物量积累（p < 0.05）。这些发现突出了R. mangle相关细菌作为生物肥料的潜力，并强调了包括安全性评估和详细功能表征的重要性，以确保它们在可持续农业系统中负责任的使用。

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引用次数: 0

Comparative genomic analysis of underutilized legumes: insights into evolutionary relationships, genome evolution and stress tolerance 未充分利用的豆科植物的比较基因组分析：对进化关系，基因组进化和胁迫耐受性的见解

Q1 Immunology and Microbiology

Biotechnology Reports

Pub Date : 2025-08-21 DOI: 10.1016/j.btre.2025.e00918

Omena Bernard Ojuederie , Ufuoma Lydia Akpojotor , Adetomiwa Ayodele Adeniji , Tina Chukwuyem Ojuederie , Jacob Olagbenro Popoola , Olubukola Oluranti Babalola

African yam bean, Mung bean, and Winged bean, which are rich sources of nutrients and bioactive compounds, offer significant potential for food and nutrition security, yet they are underutilized. A comparative genomic analysis of these legumes with cowpea was conducted to unearth their molecular architecture and uncover their rich genomic profile. Protein and genomic fasta sequences were retrieved from the GenBank of the NCBI, and orthologous genes investigated, and secondary metabolites determined using OrthoVenn3 and PlantiSMASH programs. A total of 7761 single-copy and 20,250 unique genes were identified, which revealed their genetic diversity and conservation. Phylogenetic analysis showed the closest relationship between Cowpea and Mung bean, with Winged bean diverging significantly. Cowpea and Mung bean had significant gene expansions (+1051), while African yam bean (-864) and Winged bean (-643) had substantial gene losses. GO enrichment revealed the contributions to adaptations in the different legume species to biotic and abiotic stresses, highlighting their potential as climate-resilient crops. The highest protein gene (enzyme) count for saccharide (68) and terpene (18) biosynthesis was obtained in AYB. At the same time, mung bean had the highest gene clusters for alkaloids (10) and polyketides (5), and the highest enzyme count for the biosynthesis of alkaloids (32) and polyketides (17). Underutilized legumes exhibited higher essential amino acid levels compared to cowpea. These findings provide valuable insights for breeding programs and biotechnological interventions to improve the nutritional value and acceptance of these underutilized legumes, ultimately contributing to food and nutrition security.

非洲山药豆、绿豆和翼豆是营养物质和生物活性化合物的丰富来源，为粮食和营养安全提供了巨大潜力，但尚未得到充分利用。对这些豆科植物与豇豆进行了基因组比较分析，揭示了它们的分子结构，揭示了它们丰富的基因组图谱。从NCBI的GenBank中检索蛋白质和基因组fasta序列，研究同源基因，并使用OrthoVenn3和PlantiSMASH程序测定次级代谢物。共鉴定出7761个单拷贝基因和20250个独特基因，揭示了它们的遗传多样性和保守性。系统发育分析表明，豇豆与绿豆亲缘关系最密切，有翼豆有明显的分化。豇豆和绿豆基因扩增显著（+1051），而非洲山药豆（-864）和翼豆（-643）基因缺失显著。氧化石墨烯的富集揭示了不同豆科植物对生物和非生物胁迫的适应性，突出了它们作为气候适应型作物的潜力。糖（68个）和萜烯（18个）生物合成蛋白基因（酶）计数最高。同时，绿豆中生物碱（10个）和聚酮（5个）基因簇数最多，生物碱（32个）和聚酮（17个）生物合成酶数最多。与豇豆相比，未充分利用的豆类显示出更高的必需氨基酸水平。这些发现为育种计划和生物技术干预提供了有价值的见解，以提高这些未充分利用的豆类的营养价值和接受度，最终促进粮食和营养安全。

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引用次数: 0

Investigating a new Dillenia ovata endophytic bacteria to produce antioxidants and anti-diabetes activity in vitro and in vivo 研究一种新的卵形莲内生细菌在体外和体内产生抗氧化剂和抗糖尿病活性

Q1 Immunology and Microbiology

Biotechnology Reports

Pub Date : 2025-08-20 DOI: 10.1016/j.btre.2025.e00921

Linh Chi Tran , Chong Kim Thien Duc , Tuan Trong Nguyen , Duy Toan Pham , Danh Thai Luu , Trang Thi Xuan Dai

A novel endophytic bacterium, Bacillus sp. DO-R5, with strong antioxidant and anti-diabetic properties, was isolated from Dillenia ovata, a traditional Vietnamese medicinal plant. Bacillus sp. DO-R5 was identified using morphological characteristics, SEM, and 16S rRNA sequencing, demonstrated the highest bioactivity among the 19 isolates. Optimal fermentation conditions (8.9 g/L D-glucose, pH 7.5, 38 °C, and 70.5 h) were determined using the Box-Behnken design. Under these conditions, Bacillus sp. DO-R5 significantly enhanced the production of bioactive compounds including diosmin, rutin, and chlorogenic acid, and exhibited improved in vitro antioxidant, anti-inflammatory, and anti-diabetic activities. In vivo, the extract effectively reduced blood glucose, regulated lipid profiles, supported weight control, and protected organs. These findings emphasize the potential therapeutic use of D. ovata endophytic bacteria as a natural source of anti-diabetic drugs.

摘要从越南传统药用植物紫莲中分离到一株具有较强抗氧化和抗糖尿病活性的内生细菌DO-R5。通过形态学特征、扫描电镜（SEM）和16S rRNA测序对DO-R5菌株进行鉴定，发现其生物活性最高。采用Box-Behnken设计确定最佳发酵条件（8.9 g/L D-glucose, pH 7.5, 38°C, 70.5 h）。在这些条件下，芽孢杆菌sp. DO-R5显著提高了地奥米、芦丁和绿原酸等生物活性物质的产量，并表现出较好的体外抗氧化、抗炎和抗糖尿病活性。在体内，提取物有效地降低血糖，调节血脂，支持体重控制和保护器官。这些发现强调了ovata内生细菌作为抗糖尿病药物的天然来源的潜在治疗用途。

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引用次数: 0