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Isolation and Screening of Potential Lignocellulolytic Microorganisms from Rubber Bark and Other Agricultural Residues 橡胶皮及其他农业废弃物中潜在木质纤维素降解微生物的分离与筛选
Q3 Multidisciplinary Pub Date : 2017-06-15 DOI: 10.14456/VOL15ISS6PP%P
Phat Sakpetch, A. H-Kittikun, A. Chandumpai
Lignocellulolytic microorganisms were isolated from cow manure, the soil from naturally grown bamboo, the piles of rubber bark and the composting piles. The samples were collected at 9 different locations which were 5 cm deep from the surface. The basal medium (BM) containing CMC (carboxymethyl cellulose) or xylan as a carbon source and the lignin modifying basal medium (LBM) with ABTS {2,2¢-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid} or DMP (2,6-dimethoxyphenol) as an inducer were used for the primary screening of microorganisms producing cellulase, xylanase, laccase and manganese peroxidase, respectively. Forty-five bacterial isolates (B), twenty-five isolates of actinomyces (A) and fifteen fungal isolates (F) were obtained. After that twelve isolates were selected to evaluate enzyme production in LBM containing decomposed rubber bark as a carbon source at 30 °C and at 45 °C under aerobic conditions (150 rpm shaking). The isolates F6, A2 and B15 displayed the highest enzyme activities with a CMCase activity of 0.61, 0.33 and 0.21 U/mL, respectively. Moreover, they also produced xylanase with an activity of 0.61, 0.36 and 0.32 U/mL, respectively. However, only the isolate F6 showed laccase activity and manganese peroxidase activity of 0.81 and 0.56 U/mL, respectively. The isolates B15 and A2 were identified by 16S rDNA with 99 % similarity to Bacillus subtilis and Streptomyces thermovulgaris , respectively. Aside from that, isolate F6 was identified by 18S rDNA with 100 % similarity to Trichoderma asperellum . When these microorganisms were grown in the LBM broth with 10 % decomposed rubber bark they produced the highest lignocellulolytic enzymes in the stationary phase of growth.
从牛粪、天然竹子的土壤、成堆的橡胶树皮和堆肥堆中分离出木质纤维素分解微生物。样品在离地表5厘米深的9个不同位置采集。分别以羧甲基纤维素(CMC)或木聚糖为碳源的基础培养基(BM)和以ABTS{2,2ⅱ-二(3-乙基苯并噻唑啉-6-磺酸)或DMP(2,6-二甲氧基酚)为诱导剂的木质素改性基础培养基(LBM)对产纤维素酶、木聚糖酶、漆酶和锰过氧化物酶的微生物进行了初步筛选。得到45株细菌分离株(B)、25株放线菌分离株(A)和15株真菌分离株(F)。然后,选择12个分离株,在30°C和45°C有氧条件下(150 rpm摇动),以分解的橡胶树皮为碳源,评估LBM的酶产量。菌株F6、A2和B15的CMCase活性最高,分别为0.61、0.33和0.21 U/mL。产木聚糖酶的活性分别为0.61、0.36和0.32 U/mL。只有F6的漆酶和锰过氧化物酶活性分别为0.81和0.56 U/mL。经16S rDNA鉴定,分离物B15和A2分别与枯草芽孢杆菌和热柳链霉菌具有99%的相似性。此外,分离物F6经18S rDNA鉴定与曲霉木霉的相似性为100%。当这些微生物在含有10%分解橡胶树皮的LBM肉汤中生长时,它们在生长的固定阶段产生了最高的木质纤维素水解酶。
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引用次数: 2
Modeling Network Evolution by Colored Petri Nets 基于有色Petri网的网络演化建模
Q3 Multidisciplinary Pub Date : 2016-11-09 DOI: 10.14456/VOL14ISS10PP%P
Suwimon Vongsingthong, Sirapat Boonkrong, H. Unger
Discovering how information was distributed was essential for tracking, optimizing, and controlling networks. In this paper, a premier approach to analyze the reciprocity of user behavior, content, network structure, and interaction rules to the interplay between information diffusion and network evolution was proposed. Parameterization and insight diffusion patterns were characterized based on the community structure of the underlying network using diffusion related behavior data, collected by a developed questionnaire. The user roles in creating the flow of information were stochastically modeled and simulated by Colored Petri Nets, where the growth and evolution of the network structure was substantiated through the formation of the clustering coefficient, the average path length, and the degree distribution. This analytical model could be used for various tasks, including predicting future user activities, monitoring traffic patterns of networks, and forecasting the distribution of content.
发现信息如何分布对于跟踪、优化和控制网络至关重要。本文提出了一种分析用户行为、内容、网络结构和交互规则对信息扩散与网络演化相互作用的互惠性的初步方法。基于底层网络的社区结构,利用开发的问卷收集的扩散相关行为数据,对参数化和洞察扩散模式进行表征。利用有色Petri网对用户在信息流创建过程中的角色进行随机建模和模拟,通过形成聚类系数、平均路径长度和度分布来证实网络结构的增长和演变。这个分析模型可以用于各种任务,包括预测未来的用户活动、监控网络的流量模式和预测内容的分布。
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引用次数: 2
In Vitro Laticifer Identification in Young Shoot-Derived Callus of Hevea brasiliensis Muell. Arg. 巴西橡胶树幼芽愈伤组织乳汁管的体外鉴定。参数。
Q3 Multidisciplinary Pub Date : 2016-10-10 DOI: 10.14456/VOL14ISS11PP%P
Yurachat Yodyotee, Peerapat Roongsattham, C. Nualsri, U. Meesawat
In vitro laticifer in young shoot-derived callus of Hevea brasiliensis Muell. Arg. (RRIM600 cultivar) was examined histologically and histochemically. Calluses were induced on a 2,4-D (1 mgL -l ) supplemented Murashige and Skoog (MS) culture medium containing different strengths of MS-micronutrients (quarter (¼), half (½) and full) and kinetin (0, 1 and 2 mgL -1 ). The highest percentage of callus induction (52.78 ± 5.01 %) exhibiting hard and compact calluses was achieved using ½-strength MS-micronutrients without kinetin. Friable calluses were obtained on full-strength MS-micronutrients supplemented with 1 mgL -1 kinetin. Histological details indicated that calluses initiated from the sub-epidermal layers. One-month-old calluses presented thick cell wall laticifers which were mainly randomly arranged and located in separately and aggregately. All in vitro laticifers in the callus were non-elongated which were different from those in the stem. Some callus cells exhibited secondary wall thickening which had structural features similar to those of the vessel.
巴西橡胶树幼芽愈伤组织的离体乳汁管。参数。(RRIM600品种)进行组织学和组织化学检查。在含有不同浓度的MS微量元素(1 / 4、1 / 2和1 / 2)和kinetin(0、1和2 mg / 1)的培养基中,添加2,4- d (1 mg / l)的Murashige和Skoog (MS)培养基诱导出愈伤组织。1 / 2强度ms微量营养素不含动蛋白的愈伤组织诱导率最高(52.78±5.01%),形成坚硬致密的愈伤组织。在添加1 mg -1激动素的全强度ms微量营养素中获得脆性愈伤组织。组织学细节表明,愈伤组织起源于亚表皮层。1月龄愈伤组织呈现较厚的细胞壁乳汁管,乳汁管以随机排列为主,有单独分布的,也有聚集分布的。愈伤组织的离体乳汁管均不伸长,与茎中的乳汁管不同。一些愈伤组织细胞表现出次生壁增厚,其结构特征与血管相似。
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引用次数: 3
Electrical Characterization by Impedance Spectroscopy of Double Perovskites of Y2NiMnO6 Ceramics 双钙钛矿Y2NiMnO6陶瓷的阻抗谱表征
Q3 Multidisciplinary Pub Date : 2016-04-11 DOI: 10.14456/VOL13ISS12PP%P
T. Sareein, N. Albutt
Impedance spectroscopy was used in order to investigate the electric properties of double perovskites of the Y 2 NiMnO 6 ceramics, which were prepared by thermal decomposition at 800 °C for 6 h followed by sintering at 1400 °C for 6, 12, 18 and 24 h. Consequently, the electric characterization of the Y 2 NiMnO 6 ceramics was performed at temperature from -50 to 200 °C, in the frequency range from 10 2 to 10 8 Hz. Results in the activation energy relaxation ( ) are significantly increased from 0.1723 to 0.3813 eV and the conductivity activation energy ( ) in the grain boundary are dramatically increased from approximately 0.3599 to 0.6260 eV at 24 h. Dispersion was observed in the variation of impedance values with frequency. Possible reasons for these observations are discussed.
采用阻抗谱法研究了在800℃下热分解6 h,在1400℃下烧结6、12、18和24 h制备的双钙钛矿型y2 nimno6陶瓷的电学性能。在-50 ~ 200℃温度范围内,在10.2 ~ 10.8 Hz频率范围内对y2 nimno6陶瓷进行了电学表征。结果表明,24 h时晶界电导率活化能()从0.1723 eV显著增加到0.3813 eV,晶界电导率活化能()从约0.3599 eV显著增加到0.6260 eV,阻抗值随频率变化呈色散现象。讨论了这些观察结果的可能原因。
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引用次数: 2
Characterization and Secretive Expression in Bacillus subtilis of Endoglucanase from Bacillus safensis Isolated from Freshwater Swamp Forest 淡水沼泽森林沙芽孢杆菌内切葡聚糖酶的鉴定及其在枯草芽孢杆菌中的隐秘表达
Q3 Multidisciplinary Pub Date : 2016-03-05 DOI: 10.14456/VOL14ISS5PP%P
P. Kanchanadumkerng, M. Sakka, K. Sakka, C. Wiwat
Bacillus safensis M3 was newly isolated from freshwater swamp forest soil in western Thailand. The endoglucanase gene of B. safensis M3, cel9A, had an open reading frame of 1,848 bp encoding a 616 amino acid protein. Initial expression in Escherichia coli yielded a low amount of soluble protein in the cytosolic and secreted fractions. Cel9A was successfully expressed by recombinant B. subtilis with a 4-fold greater total enzyme activity than from recombinant E. coli . By SDS-PAGE analysis, the molecular weight of Cel9A was estimated to be 70 kDa. The optimal temperature of Cel9A was 55 °C and the optimal pH was 5 - 8. Cel9A had the highest activity in the pH range from 5 - 8, and the highest stability in pH range 4 to 10, which is useful for industrial applications. Notably, Cel9A was able to hydrolyze both mixed linkage glucan (lichenan) and hemicellulose (konjac glucomannan and oat spelt xylan) better than carboxymethylcellulose. Cel9A also showed a tolerance to metal ions and surfactants. In addition, recombinant B. subtilis with endoglucanase activity has potential for biotechnological applications and benefits in the optimization of large scale enzyme production with minimal medium using agriculture wastes and other inexpensive feedstock materials.
沙芽孢杆菌M3是从泰国西部淡水沼泽森林土壤中新分离到的。沙螺旋藻M3内切葡聚糖酶基因cel9A全长1848bp,编码616个氨基酸的蛋白。在大肠杆菌中的初始表达在细胞质和分泌部分产生少量的可溶性蛋白。重组枯草芽孢杆菌成功地表达了Cel9A,其总酶活性比重组大肠杆菌高4倍。通过SDS-PAGE分析,Cel9A的分子量估计为70 kDa。Cel9A的最佳温度为55℃,最佳pH为5 ~ 8。Cel9A在pH值5 ~ 8范围内具有最高的活性,在pH值4 ~ 10范围内具有最高的稳定性,可用于工业应用。值得注意的是,Cel9A对混合链接葡聚糖(荔枝)和半纤维素(魔芋葡甘露聚糖和燕麦木聚糖)的水解能力均优于羧甲基纤维素。Cel9A还表现出对金属离子和表面活性剂的耐受性。此外,具有内切葡聚糖酶活性的重组枯草芽孢杆菌具有潜在的生物技术应用潜力,并有利于优化利用农业废物和其他廉价原料,以最少的培养基大规模生产酶。
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引用次数: 15
The Development of Structural Equation Model of Critical Thinking among Nursing Students 护生批判性思维结构方程模型的发展
Q3 Multidisciplinary Pub Date : 2016-02-08 DOI: 10.14456/VOL14ISS1PP%P
Kanjana Srisawad, Thanin Ratanaolarn, Krissana Kiddee
These were to study the critical thinking of nursing students, and to develop and check the goodness of fit of the Structural Equation Model of critical thinking among nursing students, which was compared with an empirical data set. There were 549 first year nursing students. Multi-stage random sampling was used in this research, by cluster random sampling from 20 private higher education institutions that provide nursing courses, comprising 25 percent, and random sampling 5 private higher education institutions, in order to gain 549 samples for this study. These were critical thinking questionnaires and variable effects of critical thinking questionnaires. The data was analyzed by descriptive statistics and the Structural Equation Model. This research indicated that the goodness of fit test of the Structural Equation Model of critical thinking among nursing students was applicable. The results showed the following statistical values: Chi-square = 284.895, df = 248, p-value = 0.054, RMSEA = 0.016, RMR = 0.009, GFI = 0.968, CFI = 0.997 and c 2 /df = 1.149; this indicated that the Structural Equation Model goodness of fit matched the empirical data quite well. The Self Efficiency, Emotional Intelligence, and Learning Style variables had positively direct effects on critical thinking ( b = 0.371, 0.370 and 0.376). The Internal Locus of Control had a negatively direct effect on critical thinking ( b = -0.120). In conclusion, in theoretical and practical teaching, nursing academies should promote critical thinking among nursing students by using the following variables: self-efficiency, emotional intelligence, learning style, and internal locus of control. This model should be used to develop complementary courses on critical thinking for nursing students.
目的是研究护生批判性思维的现状,建立护生批判性思维结构方程模型并检验其拟合优度,并与实证数据集进行比较。共有549名一年级护理专业学生。本研究采用多阶段随机抽样方法,对20所开设护理课程的私立高等教育机构进行整群随机抽样,占25%;对5所私立高等教育机构进行随机抽样,共获得549个样本。这些是批判性思维问卷和变量效应批判性思维问卷。采用描述性统计和结构方程模型对数据进行分析。本研究表明护生批判性思维结构方程模型的拟合优度检验是适用的。结果显示:χ 2 = 284.895, df = 248, p值= 0.054,RMSEA = 0.016, RMR = 0.009, GFI = 0.968, CFI = 0.997, c2 /df = 1.149;这表明结构方程模型的拟合优度与经验数据吻合较好。自我效率、情绪智力和学习风格对批判性思维有直接正向影响(b = 0.371、0.370和0.376)。内在控制源对批判性思维有负向直接影响(b = -0.120)。综上所述,在理论和实践教学中,护理院校应通过自我效能、情绪智力、学习风格和内在控制源等变量来促进护生批判性思维的培养。这一模式应用于为护理学生开发批判性思维的补充课程。
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引用次数: 2
Relationship between Poor Sleep Quality and Psychological Problems among Undergraduate Students in the Southern Thailand. 泰国南部大学生睡眠质量差与心理问题的关系
Q3 Multidisciplinary Pub Date : 2016-01-01
Wipawan C Pensuksan, Somrat Lertmaharit, Vitool Lohsoonthorn, Thanapoom Rattananupong, Thanawan Sonkprasert, Bizu Gelaye, Michelle A Williams

We conducted this study to fill the noted gap in the available literature by evaluating the prevalence of poor sleep quality in this understudied population and to explore the relationship between sleep quality and psychological problems among undergraduates in Thailand. This study used a cross-sectional survey. Self-administrated questionnaires included the Pittsburgh Sleep Quality Index (PSQI), the Epworth Sleepiness Scale, the Depression, Anxiety and Stress Scale and the Thai General Health Questionnaire. The study group included 1,055 undergraduates, aged 18-25 years. The prevalence of poor sleep quality was 42.4%. Students classified as poor quality sleepers reported significantly more psychological problems, indicating a linear trend of progressively worse global sleep quality associated with greater mood and anxiety symptomatology. Prospective studies that include objective measures of sleep duration and quality are needed to more fully develop focused health promotion strategies for Southeast Asian undergraduates.

我们进行这项研究是为了填补现有文献中的空白,通过评估这一未被充分研究的人群中睡眠质量差的普遍程度,并探讨泰国大学生睡眠质量与心理问题之间的关系。这项研究采用了横断面调查。自我管理问卷包括匹兹堡睡眠质量指数(PSQI)、Epworth嗜睡量表、抑郁、焦虑和压力量表和泰国一般健康问卷。研究小组包括1055名年龄在18-25岁之间的大学生。睡眠质量差的患病率为42.4%。被归类为睡眠质量差的学生报告了更多的心理问题,这表明全球睡眠质量逐渐恶化的线性趋势与更大的情绪和焦虑症状有关。需要前瞻性研究,包括客观测量睡眠时间和质量,以更充分地为东南亚大学生制定有针对性的健康促进策略。
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引用次数: 0
A Study on Silk and Its Mixed Fabric for Functional Properties 丝绸及其混纺织物功能性能的研究
Q3 Multidisciplinary Pub Date : 2015-11-19 DOI: 10.14456/VOL13ISS11PP%P
Jeyakodi Moses Jeyaraj, M. Arumugam, Vellingiri Kulandaiappan
Silk is a valuable protein fiber in the textile industry. It is used for delicate applications in many areas, such as in sarees, suitings, curtains, and luxurious interiors. Lyocell textiles are categorized as cellulose polymeric products of natural fibers. They have their individual uses in the textile, apparel, and garment fields. Polyester (PET) is a synthetic textile with very good resistance towards chemical and microbial attacks. To diversify its properties and usages, silk is mixed with polyester and lyocell. The fabrics are dyed with both natural dyes (kum kum, indigo, bar berry) and synthetic dyes (reactive dye (H), reactive dye (M), and sulfur dye). This mixed fabric is compared with 100 % silk for some of the basic properties, like k/s value, fastness property, antimicrobial property, air permeability, UV-protection behavior, and SEM analysis. The silk mixed fabric gives appreciable results compared with the 100 % silk fabric.
丝绸在纺织工业中是一种有价值的蛋白质纤维。它被用于许多精致的应用领域,如纱丽、西装、窗帘和豪华的室内装饰。莱赛尔纺织品被归类为天然纤维的纤维素聚合物产品。它们在纺织、服装和服装领域有各自的用途。聚酯(PET)是一种抗化学和微生物侵害的合成纺织品。为了使其性能和用途多样化,丝绸与聚酯和莱赛尔混合在一起。织物是用天然染料(kum kum,靛蓝,棒莓)和合成染料(活性染料(H),活性染料(M)和硫染料)染色的。将这种混纺织物的一些基本性能与100%真丝进行比较,如k/s值、牢度、抗菌性能、透气性、防紫外线性能和扫描电镜分析。真丝混纺织物与纯真丝织物相比,效果明显。
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引用次数: 5
Performance and Carcass Characteristics of Rabbits Fed Oil Supplemented Diets 油饲粮对家兔生产性能和胴体特性的影响
Q3 Multidisciplinary Pub Date : 2015-10-23 DOI: 10.14456/VOL13ISS1PP%P
J. A. Omar, A. Zaza
The objective of this study is to investigate the effects of feeding different dietary fat supplements in the finisher rations of Baladi rabbits, including sesame oil (SO), olive oil sediments (OOS), and poultry grease (PG), in comparison to the traditional oil supplement, the soybean soap stock oil (SS), on growth performance, blood lipid profile, dressing percentage and carcass cut, and meat quality: water holding capacity (WHC) and cell forming unit (CFU). A total of 48 Baladi rabbits were used, with individual body weights (BW) of 519 ± 22 g at the beginning of the experiment. Rabbits were randomly divided into 4 groups of 12, and those in each group were individually fed cereal grain-soybean meal (SBM) with a fixed amount ( i.e . 30 g/kg dry matter (DM)) of oil, being either soybean oil (SOY), olive oil sediments (OOS), recycled restaurant oil (RRO), or poultry grease (PG). All rations were isonitrogenous and contained iso-metabolizable energy (ME). At the end of the 44 day feeding trial, all animals were slaughtered. Rabbits fed a SOY supplemented diet consumed more (P < 0.05) feed than those fed the OOS, RRO, or PG supplemented diets. However, rabbits fed the SOY had a better (P < 0.05) feed conversion ratio than rabbits fed the OOS, PG, or RRO diets. Oil source had no effect on carcass components weights. Liver was heavier (P < 0.05) in rabbits fed the SOY supplemented diet. However, the RRO fed rabbits had heavier (P < 0.05) small intestine, large intestine and cecum. In conclusion, the positive effects of the tested oil supplements ( i.e. RRO, OOS, PG) on the studied performance and carcass traits is encouraging, but more investigation is needed to identify the optimal levels for these supplements in various diets of local rabbits. doi: 10.14456/WJST.2016.10
本试验旨在研究在巴拉迪兔的育肥粮中添加香油(SO)、橄榄油沉淀物(OOS)和家禽油脂(PG),并与传统油脂添加物大豆皂原油(SS)相比,对巴拉迪兔的生长性能、血脂、屠宰率和胴体切除率以及肉质、保水能力(WHC)和细胞形成单位(CFU)的影响。试验选用Baladi兔48只,试验开始时体重为519±22 g。试验将家兔随机分为4组,每组12只,每组分别饲喂固定剂量的谷物-豆粕(SBM)。30 g/kg干物质(DM)油,可为大豆油(SOY)、橄榄油沉淀物(OOS)、回收餐厅油(RRO)或家禽油脂(PG)。所有日粮均为等氮和等代谢能(ME)。44 d饲养试验结束时,屠宰所有动物。大豆饲粮比OOS、RRO和PG饲粮消耗更多(P < 0.05)。大豆饲粮的饲料转化率高于OOS、PG和RRO饲粮(P < 0.05)。油源对胴体组分重无影响。添加大豆的家兔肝脏较对照组重(P < 0.05)。而RRO饲喂家兔小肠、大肠和盲肠较重(P < 0.05)。综上所述,所测试的油补充剂(即RRO, OOS, PG)对所研究的生产性能和胴体性状的积极影响令人鼓舞,但需要更多的研究来确定这些补充剂在不同地方兔日粮中的最佳水平。doi: 10.14456 / WJST.2016.10
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引用次数: 3
Genetic Diversity in Slender Walking Catfish (Clarias nieuhofii) Populations: Implications for Population Management 细长行走鲶鱼(Clarias nieuhofii)种群遗传多样性:种群管理意义
Q3 Multidisciplinary Pub Date : 2015-10-23 DOI: 10.14456/VOL13ISS6PP%P
Jamjun Pechsiri, A. Vanichanon
Random amplified polymorphic DNA (RAPD) markers were applied to assess the genetic diversity and population structure of the slender walking catfish ( Clarias nieuhofii , Clariidae) from 3 wild populations in peat swamp forests in southern Thailand (Surat Thani , Narathiwat, and Phatthalung). The selected 14 RAPD primers produced 105 RAPD bands, ranging from 6 to 11 bands per primer, and ranging in size between 400 and 3,000 bp. The percentage of polymorphic loci, gene diversity and Shannon’s information index values were 75.00 %, 0.2252, and 0.3443 for Surat Thani; 86.59 %, 0.2982, and 0.4441 for Narathiwat, and 96.25 %, 0.3371, and 0.5049 for Phatthalung, respectively. Among the 3 populations, the highest genetic distance (0.2213) was found between the Narathiwat and Surat Thani populations. High genetic differentiation ( G st = 0.2815) was detected in 3 populations with low gene flow ( Nm = 1.2762) among the overall populations. The clustering pattern obtained by the unweighted pair-group (UPGMA) method separated the C. nieuhofii samples into 3 groups, but 2 clusters. The results indicated a high level of genetic variation and genetic differentiation among C. nieuhofii from different populations in southern Thailand. This information would be useful to construct appropriate breeding programs, and could help conserve populations used as potential sources for stock management, restocking programs, and sustainable uses.
采用随机扩增多态性DNA (RAPD)标记对泰国南部素叻他尼、那拉提瓦和Phatthalung泥炭沼泽森林3个野生种群的细长行走鲶鱼(Clarias nieuhofii, Clariidae)的遗传多样性和种群结构进行了分析。选取的14条RAPD引物共产生105条RAPD条带,每个引物有6 ~ 11条条带,长度在400 ~ 3000 bp之间。素叻他尼的多态性位点百分率、基因多样性和香农信息指数分别为75.00%、0.2252和0.3443;那拉提瓦为86.59%、0.2982、0.4441,帕塔隆为96.25%、0.3371、0.5049。在3个居群中,那拉提瓦居群与素叻他尼居群的遗传距离最高,为0.2213。3个群体遗传分化程度高(G st = 0.2815),基因流量低(Nm = 1.2762)。UPGMA法得到的聚类模式将nieuhofii样本分为3组,但分为2个聚类。结果表明,泰国南部不同种群的纽霍菲梭菌存在高度的遗传变异和遗传分化。这些信息将有助于建立适当的育种计划,并有助于保护种群,作为种群管理、再放养计划和可持续利用的潜在资源。
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引用次数: 1
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Walailak Journal of Science and Technology
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