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A Simple and Rapid HPLC Technique for Determination of Arecoline in Areca Nut (Areca catechu L.) Extract 快速高效液相色谱法测定槟榔果中槟榔碱的含量提取
Q3 Multidisciplinary Pub Date : 2013-01-12 DOI: 10.2004/WJST.V10I1.439
C. Jantarat, P. Sirathanarun, Wilaihong Songsrm, Wititta Srinornate, Sujittra Daengprom
A simple and rapid high-performance liquid chromatographic (HPLC) method for determination of arecoline extract from areca nut is reported. Arecoline is a major component cholinomimetic alkaloid found in areca nuts. It was extracted from dried seed powder to obtain high purity before analysis. The contents of arecoline in unripe and ripe areca nuts were compared. The analytical conditions for reverse-phase HPLC with UV detection were as follows: column, a 250 × 4.6 mm I.D., particle size 5 mm, Inertsil ® ODS-3 (GL sciences Inc., Tokyo, Japan); mobile phase, a 88:12 (%v/v) mixture of acetonitrile: phosphate buffer (pH 5.9); column temperature, 25 °C; flow rate, 1 mL/min; and detection at 254 nm. The retention time of arecoline was 5.0 min. The calibration curve of the method was linear (r 2 > 0.99) in the 10 - 200 mg/mL range. Method inter-day precision (RSD) was 0.42 - 1.15 %, and % recovery was 103.23 ± 5.76 % indicating high precision and accuracy of the method. The contents of arecoline in unripe and ripe areca nuts were 0.1434 ± 0.0016 and 0.0944 ± 0.0002 %w/w of dried seed powder, respectively. The method was simple, rapid, precise, accurate and selective to determine arecoline in areca nut extract.
建立了一种简便、快速的高效液相色谱法测定槟榔果中槟榔碱的含量。槟榔碱是在槟榔果中发现的一种主要的仿胆碱类生物碱。在分析前从干燥的种子粉末中提取,以获得高纯度。比较了熟槟榔和未熟槟榔中槟榔碱的含量。反相高效液相色谱-紫外检测的分析条件为:色谱柱,内径250 × 4.6 mm,粒径5 mm, Inertsil®ODS-3 (GL sciences Inc., Tokyo, Japan);流动相:88:12 (%v/v)的乙腈:磷酸盐缓冲液(pH 5.9);柱温,25℃;流量:1 mL/min;在254 nm处进行检测。槟榔碱的保留时间为5.0 min。在10 ~ 200 mg/mL范围内,该方法的校准曲线呈线性关系(r = 2 ~ 0.99)。方法日间精密度(RSD)为0.42 ~ 1.15%,回收率为103.23±5.76%,具有较高的精密度和准确度。未成熟和成熟槟榔果中槟榔碱含量分别为干籽粉的0.1434±0.0016和0.0944±0.0002 %w/w。该方法简便、快速、精密度高、准确度高、选择性好,可用于槟榔提取物中槟榔碱的测定。
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引用次数: 4
Tree Species Composition, Diversity and Above Ground Biomass of Two Forest Types at Redang Island, Peninsula Malaysia 马来西亚半岛热当岛两种森林类型的树种组成、多样性和地上生物量
Q3 Multidisciplinary Pub Date : 2012-12-13 DOI: 10.2004/WJST.V10I1.239
M. Khairil, M. Nashriyah, N. Norhayati, Shahril Amin, N. Fatihah
A study was conducted to determine the tree species composition, diversity and above ground biomass at Redang Island, Terengganu. Two plots of 0.1 ha were established at the inland forest and coastal forest of the island. As the result, a total of 387 trees ≥ 5 diameters at breast height (DBH) were recorded. The coastal forest recorded 167 individuals representing 48 species from 37 genera and 26 families while the inland forest had 220 individuals representing 50 species from 43 genera and 25 families. Shorea glauca (Dipterocarpaceae) was the most important species at the coastal forest with a Species Importance Value Index ( SIV i ) of 10.5 % while Dipterocarpus costulatus (Dipterocarpaceae) was the most important species at the inland forest with 13.8 %. Dipterocarpaceae was the most important family in both forest plots with FIV i at 20.4 % in the coastal and 21.5 % in the inland forest. The Shannon-Weiner Diversity Index (H’) was considered high in both forest plots with 3.4 (H’ max = 3.9) at the coastal forest and 3.5 (H’ max = 4.0) at the inland forest. Sorenson’s Community Similarity Coefficient (CCs) showed that tree species communities between the two forest plots had moderate similarity with CC = 0.5. The Shannon Evenness Index ( J ’) in the two forest plots was 0.89. The total above ground biomass at the coastal forest was 491 t/ha and at the inland forest it was 408 t/ha. From all the species recorded in this study, 11 species were listed as threatened species by IUCN Red Data Book, of which four were listed as endangered and critically endangered, six were listed as lower risk and one species was listed as vulnerable.
对丁加奴热当岛的树种组成、多样性和地上生物量进行了研究。在岛上的内陆森林和沿海森林建立了两个0.1 ha的样地。结果,共记录到胸径≥5径的乔木387棵。沿海林有26科37属48种167只,内陆林有25科43属50种220只。物种重要值指数(SIV i)为10.5%,是沿海森林最重要的物种,而内陆森林最重要的物种是双龙桃(Dipterocarpus costulatus),为13.8%。在两个样地中,双龙心科是最重要的科,其FIV i分别为沿海的20.4%和内陆的21.5%。两个样地的Shannon-Weiner多样性指数(H’)均较高,滨海林样地为3.4 (H’max = 3.9),内陆林样地为3.5 (H’max = 4.0)。Sorenson群落相似系数(CCs)表明,两个样地之间的树种群落具有中等的相似性,CC = 0.5。两个样地的香农均匀度指数(J’)为0.89。沿海森林地上总生物量为491 t/ha,内陆森林地上总生物量为408 t/ha。在本研究记录的所有物种中,有11种被IUCN红皮书列为受威胁物种,其中4种被列为濒危和极危物种,6种被列为低危物种,1种被列为易危物种。
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引用次数: 4
Effects of Synbiotic (Biomin Imbo) on Growth Performance, Survival Rate, Reproductive Parameters of Angelfish (Pterophyllum scalare) 合成制剂(Biomin Imbo)对天使鱼生长性能、存活率及繁殖参数的影响
Q3 Multidisciplinary Pub Date : 2012-11-27 DOI: 10.2004/WJST.V9I4.384
Hamed Nekoubin, S. Hatefi, Susan Javahery, M. Sudagar
This study was carried out to evaluate the effect of synbiotic ( Biomin imbo ) on growth performance, survival rate and reproductive parameters of Angelfish ( Pterophyllum scalare ) via supplementation with Biomar. Four levels of Biomar experimental diets (54 % protein and 14 % lipid) were prepared by adding synbiotic (0.15, 0.5, 0.75, 1 g/Kg) at a basal diet (Biomar) and the Angelfish larvae in experimental treatments were fed four levels of synbiotic with 5 percent body weight (3 times a day). The larvae in the control treatment were fed without supplemented Biomar. The results showed that larvae fed on the synbiotic had significantly increased final body weight in comparison with the control treatment. After 90 days, 5 adult female and male Angelfish were divided from each treatment. The results showed that fecundity in experimental treatments increased in comparison with the control treatment. The synbiotic also had positive effects on hatching rate in comparison with those in the control treatment but, there were no significant differences (P > 0.05) among treatments. The synbiotic also had significant positive effects on the specific growth rate (SGR) and feed conversation efficiency (FCE) in comparison to those fed the control treatment, however treatment T3 was more than T4 (T3 > T4 > T2 > T1 > control treatment).
本试验旨在通过添加Biomar来评价生物制剂(Biomin imbo)对天使鱼(Pterophyllum scalare)生长性能、存活率和繁殖参数的影响。在基础饲粮(Biomar)中添加合成物(0.15、0.5、0.75、1 g/Kg),配制4个水平的Biomar试验饲粮(蛋白质含量为54%,脂肪含量为14%),试验处理神仙鱼仔鱼分别饲喂4个水平、5%体重的合成物(每天3次)。对照处理不添加生物马尔。结果表明,与对照处理相比,饲喂合成菌的幼虫的最终体重显著增加。90 d后,每组分别取雌性和雄性神仙鱼5只。结果表明,与对照处理相比,试验处理的繁殖力有所提高。与对照处理相比,合成菌对孵化率也有正影响,但各处理间差异不显著(P < 0.05)。与对照处理相比,合成制剂对特定生长率(SGR)和饲料转化率(FCE)也有显著的正影响,但T3处理大于T4处理(T3 > T4 > T2 > T1 >对照处理)。
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引用次数: 7
Mathematical Models of Bead-Spring Jets during Electrospinning for Fabrication of Nanofibers 静电纺丝制备纳米纤维时珠-弹簧射流的数学模型
Q3 Multidisciplinary Pub Date : 2012-11-02 DOI: 10.2004/WJST.V9I4.449
T. Dasri
Electrospinning is a popular technique to produce structures in the form of nanofibers. These nanofibers can be used for many applications such as filtration composites, insulator and energy storage. The technique is based on the electrostatic force that acts on the polymeric solution. However, during the electrospinning process the liquid jet shows unstable behavior. This problem causes the random formation of nanofibers. This article focuses on the mathematical models to describe the dynamics behavior of the fluid jet in the electrospinning process. There are a lot of different parameters in the model. Variation in these parameters results in a change in jet behaviors. This brief review is a summary of the authors’ recent work. The Reneker’s model and Wu’s model are used to describe the dynamics behavior of the jet used in electrospinning.
静电纺丝是一种生产纳米纤维结构的流行技术。这些纳米纤维可用于过滤复合材料、绝缘体和储能等许多应用。该技术是基于作用在聚合物溶液上的静电力。然而,在静电纺丝过程中,液体射流表现出不稳定的行为。这个问题导致纳米纤维的随机形成。本文重点介绍了描述静电纺丝过程中流体射流动力学行为的数学模型。模型中有很多不同的参数。这些参数的变化会导致射流行为的变化。这篇简短的综述是对作者最近工作的总结。用Reneker模型和Wu模型描述了静电纺丝中射流的动力学行为。
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引用次数: 2
Analysis of Heat and Mass Transfer Effects on an Isothermal Vertical Oscillating Plate 等温垂直振荡板的传热传质效应分析
Q3 Multidisciplinary Pub Date : 2012-10-26 DOI: 10.2004/WJST.V9I4.451
S. Sheri, Murali Gundagani, Maruthi Prasad Karanamu
The objective of this paper is to investigate the heat and mass transfer effects on an isothermal vertical oscillating plate. The dimensionless governing equations are solved using a finite element method. The velocity, temperature and concentration fields for different physical parameters like thermal Grashof number, mass Grashof number, prandtl number, Schmidt number, phase angle and time are shown graphically.
本文的目的是研究等温垂直振荡板的传热传质效应。采用有限元法求解无量纲控制方程。用图形表示了热格拉什夫数、质量格拉什夫数、普朗特数、施密特数、相位角和时间等不同物理参数下的速度场、温度场和浓度场。
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引用次数: 2
Method Evaluating the Durability of Aircraft Piston Engines 航空活塞式发动机耐久性评价方法
Q3 Multidisciplinary Pub Date : 2012-09-24 DOI: 10.2004/WJST.V9I4.315
L. Piancastelli, L. Frizziero, Eugenio Morganti, E. Pezzuti
A significant issue in aircraft engines is quantifying residual life to overhaul. The algorithm described in this paper calculates with a good level of reliability the residual life of a petrol piston engine. The method was tested on small, latest-generation, naturally-aspirated aircraft and racing piston engines, and has been effective in several experiments. This method is implemented directly on the electronic control system of the engine with very few lines of C-code. The method can also be used in many industrial engines. This innovative method assumes that only two main factors (power level and wear) affect engine durability or time between overhauls. These two factors are considered as separate and combined with worst case criteria. The wear is assumed to follow a logarithmic law and a formula similar to the Miner’s law for material fatigue is used, making it possible to calculate the power-level curve with knowledge of only two points. The wear-curve is also related to elapsed engine cycles. The algorithm is very simple and can be implemented with just a few lines of software code accessing data collected from existing sensors. The system is currently used to evaluate actual residual life of racing engines.
飞机发动机的一个重要问题是对大修前的剩余寿命进行量化。本文所描述的算法能较好地计算出汽油活塞式发动机的剩余寿命。该方法在最新一代的小型自然吸气飞机和赛车活塞发动机上进行了测试,并在几次实验中取得了成功。该方法直接在发动机的电子控制系统上实现,只需要很少的c代码行。该方法也可用于许多工业发动机。这种创新的方法假设只有两个主要因素(功率水平和磨损)影响发动机的耐用性或大修间隔时间。这两个因素被认为是单独的,并与最坏情况标准结合在一起。假设磨损遵循对数规律,并使用类似于材料疲劳的Miner定律的公式,使得仅用两点知识就可以计算功率水平曲线。磨损曲线也与经过的发动机循环有关。该算法非常简单,只需几行软件代码就可以实现从现有传感器收集的数据。该系统目前用于评估赛车发动机的实际剩余寿命。
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引用次数: 51
Solitary Wave Solutions for Zoomeron Equation Zoomeron方程的孤波解
Q3 Multidisciplinary Pub Date : 2012-09-24 DOI: 10.2004/WJST.V10I2.422
Amna Irshad, S. Mohyud-Din
Tanh-Coth Method is applied to find solitary wave solutions of the Zoomeron equation which is of extreme importance in mathematical physics. The proposed scheme is fully compatible with the complexity of the problem and is highly efficient. Moreover, suggested combination is capable to handle nonlinear problems of versatile physical nature.
Tanh-Coth方法用于求解Zoomeron方程的孤波解,这在数学物理中具有极其重要的意义。所提出的方案与问题的复杂性完全兼容,效率高。此外,建议的组合能够处理多用途物理性质的非线性问题。
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引用次数: 4
Genetic and Gestational Surrogacy: an Overview 遗传和妊娠代孕:综述
Q3 Multidisciplinary Pub Date : 2012-08-09 DOI: 10.2004/WJST.V9I3.279
Sambhu Mondal, A. Verma, Shiv K. Gupta, B. Kumar
Surrogacy is a method in which a woman bears a child for another woman. The indications for treatment include absent uterus, recurrent miscarriage, and repeated failure of in vitro fertilization (IVF) and certain medical conditions. It also defines the process in which a woman gestates a fetus, gives birth to a child, and then relinquishes her parental rights to another couple. This third party relationship to the intimate process of producing children is not a contemporary concept and has been recorded in our history in several forms. In colonial America, children were sent to other childless families to learn trades or provide extra help to the family. More recent forms of surrogacy in the U.S. are adoption, fostering, and step-parenting. In this review, various techniques, law issues, and drawbacks of surrogacy are synchronically described and postulated.
代孕是一个女人为另一个女人生孩子的一种方法。治疗的适应症包括子宫缺失、反复流产、体外受精(IVF)多次失败和某些医学条件。它还定义了一个过程,在这个过程中,一个女人怀孕,生下一个孩子,然后放弃她的父母权利给另一对夫妇。这种与生育孩子的亲密过程的第三方关系并不是一个当代概念,而是以几种形式记录在我们的历史中。在殖民时期的美国,孩子们被送到其他没有孩子的家庭学习手艺或为家庭提供额外的帮助。在美国,最近的代孕形式有收养、寄养和继父母。在这篇综述中,各种技术,法律问题,以及代孕的缺点同步描述和假设。
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引用次数: 2
Antibacterial Activity of Bauhinia sirindhorniae Extract Against Aeromonas hydrophila Isolated from Hybrid catfish 紫荆提取物对杂交鲶鱼嗜水气单胞菌的抑菌活性研究
Q3 Multidisciplinary Pub Date : 2012-07-12 DOI: 10.2004/WJST.V9I3.312
N. Nithikulworawong
The studies on antibacterial activity of Bauhinia sirindhorniae extract, an indigenous plant of Nong Khai province, against Aeromonas hydrophila isolated hybrid catfish were assayed. Root, stem, leaves and flowers of B. sirindhorniae were extracted by acetone, ethanol 50 %, ethanol 95 % and methanol. In antibacterial screening performed by disc method was conducted. The results revealed that the highest activity against A. hydrophila , found in roots extract using acetone with an inhibition zone, was 20.33 ± 0.58 mm. The minimum inhibitory concentration (MIC) for B. sirindhorniae extracts were also determined by the broth dilution method. The lowest MIC value with high efficacy against A. hydrophila was 97.66 ppm which could be obtained from the root extract of B. sirindhorniae from acetone. The minimum bactericidal concentrations (MBC) of B. sirindhorniae extracts were also determined. The determination of MBC was not observed in any concentration of B. sirindhorniae extracts.
研究了廊开省乡土植物紫荆提取物对杂交鲶鱼嗜水气单胞菌的抑菌活性。以丙酮、50%乙醇、95%乙醇和甲醇为萃取剂,分别提取金缕莲的根、茎、叶和花。采用圆盘法进行抗菌筛选。结果表明,丙酮对嗜水草根提取物的抑菌活性最高,为20.33±0.58 mm;采用肉汤稀释法测定了提取液的最低抑菌浓度(MIC)。丙酮提取的太子参根提取物对嗜水单胞菌的最低MIC值为97.66 ppm。同时测定了太子参提取物的最低杀菌浓度。在任何浓度的太子参提取物中均未见MBC的测定。
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引用次数: 7
Proximate and Fatty Acid Composition in Muscle Tissues of Rainbow Trout (Oncorhynchus mykiss) Cultured in Yazd Province of Iran 伊朗亚兹德省养殖虹鳟鱼肌肉组织的比邻脂肪酸和脂肪酸组成
Q3 Multidisciplinary Pub Date : 2012-03-28 DOI: 10.2004/WJST.V9I4.276
N. Mashaii, M. Mosaddegh, H. Sarsangi, F. Rajabipour, Ahmad Ghorghi, A. Bitaraf, H. Mozaffari‐khosravi
Iran is the number one producer of cultured coldwater fish in Asia since 2005. Rainbow trout, (Oncorhynchus mykiss) is the most common and important fish produced by Iranian fisheries. There is not enough information about carcass composition of cultured fish in Iran. Rainbow trout muscle samples were collected from six fish farms of Yazd province during February 2008. Muscle samples were frozen at -30 ο C after being homogenized. Proximate composition of samples was measured. Saturated fatty acids including palmitic, stearic, myristic, lauric acids, also unsaturated fatty acids oleic and linoleic were extracted from muscle tissue of fish at different farms, using gas chromatograghy (GC). Other unsaturated fatty acids including α-linolenic acid, Eicosa Pentaenoic Acid (EPA) and Docosa Hexaenoic Acid (DHA) had low concentrations (up to 3 %) in samples. Vitamin E levels were 4.33 - 94.34 μg/100 g.
自2005年以来,伊朗是亚洲养殖冷水鱼的头号生产国。虹鳟鱼(Oncorhynchus mykiss)是伊朗渔业生产的最常见和最重要的鱼。伊朗养殖鱼类的胴体组成资料不足。2008年2月在亚兹德省的6个养鱼场采集了虹鳟鱼肌肉样本。匀浆后的肌肉样品在-30 ο C冷冻。测量了样品的近似组成。采用气相色谱法从不同养殖场的鱼类肌肉组织中提取饱和脂肪酸,包括棕榈酸、硬脂酸、肉豆酱酸、月桂酸,以及不饱和脂肪酸油酸和亚油酸。其他不饱和脂肪酸,包括α-亚麻酸、白杨五烯酸(EPA)和白杨六烯酸(DHA)在样品中的浓度较低(高达3%)。维生素E含量为4.33 ~ 94.34 μg/100 g。
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引用次数: 3
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Walailak Journal of Science and Technology
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