Bioactive Carbohydrates and Dietary Fibre最新文献

Revealing the interaction mechanisms of characteristic aroma compounds in apples (Malus × domestica) by S-curve modeling, molecular docking, and molecular dynamics simulation 通过s曲线建模、分子对接和分子动力学模拟揭示苹果（Malus × domestica）特征香气化合物相互作用机制

IF 3.6

Bioactive Carbohydrates and Dietary Fibre

Pub Date : 2026-01-26 DOI: 10.1016/j.bcdf.2026.100523

Ning Ma , Jiancai Zhu , Heng Wang , Bingjian Shen , Michael C. Qian , Zuobing Xiao

The aroma of apples is one of the major sensory characteristics to evaluate the quality of apples. The mechanism for perceiving the aroma of apples is currently unclear. In this study, the interactions between the aroma compounds and olfactory receptors were explored by the S-curve modeling, molecular docking, and molecular dynamics simulation (MDS) technology. Hexyl acetate and β-damascenone(D = 0.46),hexyl acetate and phenethyl alcohol (D = 0.15), linalool and trans-2-hexenal (D = 0.30) etc., have synergistic effects. The molecular docking results revealed noticeable changes in the stability and affinity between the binary and single compounds combined with olfactory receptors (OR1A1 and OR2W1). In addition, molecular dynamic simulation (MDS) analysis indicated that the hydrogen bonding residues of the OR2W1-hexyl acetate were changed from ASN155 to TRP149 and ASN65 after the addition of β-damascenone. Similarly, the hydrophobic interactions of the system were increased, which played an indispensable role in forming a stable ternary system between β-damascenone-hexyl acetate and OR2W1. Meanwhile, the binding free energy decreased from −36.69 kcal/mol (hexyl acetate- OR2W1) to −37.7 kcal/mol (β-damascenone-hexyl acetate-OR2W1), which showed that the new ternary system has better stability. The results provided a theoretical basis and technical guidance for improving apple flavor and its products.

苹果的香气是评价苹果品质的主要感官特征之一。感知苹果香气的机制目前还不清楚。本研究通过s曲线建模、分子对接和分子动力学模拟（MDS）技术探讨了香气化合物与嗅觉受体之间的相互作用。乙酸己酯与β-马马酮（D = 0.46）、乙酸己酯与苯乙醇（D = 0.15）、芳樟醇与反式-2-己烯醛（D = 0.30）等均有协同作用。分子对接结果显示，与嗅觉受体（OR1A1和OR2W1）结合的二元化合物和单一化合物的稳定性和亲和力发生了显著变化。此外，分子动力学模拟（MDS）分析表明，加入β-达马ascenone后，or2w1 -乙酸己酯的氢键残基由ASN155变为TRP149和ASN65。同样，体系的疏水相互作用增加，这对于形成β-达马ascenone-hexyl acetate与OR2W1之间稳定的三元体系起着不可或缺的作用。结合自由能从−36.69 kcal/mol（醋酸己酯-OR2W1）降至−37.7 kcal/mol (β-damascenone- acetate己酯-OR2W1)，表明新三元体系具有较好的稳定性。研究结果为改进苹果风味及其产品提供了理论依据和技术指导。

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引用次数: 0

Investigation on physical modifications enhance the functional and glycaemic modulation potential of finger millet soluble dietary fiber 物理修饰对谷子可溶性膳食纤维功能和血糖调节潜能的增强研究

IF 3.6

Bioactive Carbohydrates and Dietary Fibre

Pub Date : 2026-01-24 DOI: 10.1016/j.bcdf.2026.100521

Kotinadham Sai Lahari , Sai Babu , Chagam Koteswara Reddy , Tapasya Kumari

This study investigates the effects of three physical modification techniques including ultrasonication (UMF), high pressure (HPMF), and high temperature (HTMF) on the physicochemical, functional, structural, and in vitro digestibility properties of soluble dietary fiber (SDF) extracted from finger millet. Among the treatments, ultrasonication significantly enhanced key functional properties, including water-holding capacity (19.13 g/g), swelling capacity (11.07 mL/g), oil-binding capacity (0.34 g/g), and adsorption capacities for glucose (21.24 mmol/L), cholesterol (14.55 mg/g), and sodium cholate (21.71 mg/g). In addition, ultrasonication improved the cation exchange capacity (0.87 mM/g). Structural characteriztion using Fourier-transform infrared spectroscopy (FTIR), scanning electron microscopy (SEM), and X-ray diffraction (XRD) revealed that ultrasonication increased surface porosity, disrupted fiber matrix, and exposed more number of active functional groups compared to high pressure and high temperature treatments. These structural modifications contributed to improved glucose diffusion retardation (35.22 %) and α-amylase inhibition (25.54 %), demonstrating improved glycaemic control potential. Ultrasonication's ability to generate localized cavitation effects led to efficient structural breakdown without requiring extreme thermal or pressure conditions. Overall, these results highlight ultrasonication as a promising, and eco-friendly approach for improving the nutritional and functional characteristics of millet-derived SDF, making it a valuable technique for development of high-quality, health-promoting functional food ingredients.

研究了超声（UMF）、高压（HPMF）和高温（HTMF）三种物理改性技术对谷子可溶性膳食纤维（SDF）理化、功能、结构和体外消化性能的影响。其中，超声处理显著提高了黄瓜的保水性（19.13 g/g）、溶胀性（11.07 mL/g）、油结合性（0.34 g/g）以及对葡萄糖（21.24 mmol/L）、胆固醇（14.55 mg/g）和胆酸钠（21.71 mg/g）的吸附能力。超声处理提高了阳离子交换容量（0.87 mM/g）。利用傅里叶变换红外光谱（FTIR）、扫描电镜（SEM）和x射线衍射（XRD）对纤维进行了结构表征，结果表明，与高压和高温处理相比，超声处理增加了表面孔隙率，破坏了纤维基质，暴露了更多的活性官能团。这些结构修饰有助于改善葡萄糖扩散阻滞（35.22%）和α-淀粉酶抑制（25.54%），显示出改善的血糖控制潜力。超声波能够产生局部空化效应，无需极端的高温或高压条件即可有效地破坏结构。综上所述，这些结果表明超声波技术是一种有前景的、环保的方法，可以改善小米衍生SDF的营养和功能特性，使其成为开发高质量、促进健康的功能食品配料的一种有价值的技术。

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引用次数: 0

Nutraceutical potential of low methylated pectin derived from pectin rich agrowaste 从富含果胶的农业废弃物中提取的低甲基化果胶的营养潜力

IF 3.6

Bioactive Carbohydrates and Dietary Fibre

Pub Date : 2026-01-02 DOI: 10.1016/j.bcdf.2025.100518

Rajesh Kumar, Jagan Mohan Rao Tingirikari

Increase in global population has led to significant rise in new diseases and bringing new challenges. The current treatment methods being employed are rather non-specific and have several side effects. There is a great need to find alternate, safe, prevention and treatment strategies to combat against various diseases. With global challenges such as food insecurity, increasing chronic diseases, and the pursuit of sustainable economic practices, the need for dietary fibres or nutraceuticals derived from plant waste is gaining importance. Recent studies performed on low methylated pectin (LMP) derived from pectin rich agro waste has displayed good functional properties in prevention and treatment of diseases. LMPs are generally recognized as safe, nontoxic and biocompatible. It was reported that LMPs bind to galactin-3 receptor to prevent cancer metastasis, exhibit anti-diabetic property by inhibiting α-glucosidase activity, promote anti-inflammatory activity by regulating TLR-2, IL-1β, and IL-6. LMPs exhibit antimicrobial activity by promoting the growth of beneficial bacteria, which neutralize toxins by producing short chain fatty acids. LMPs promote antioxidant activity by reducing the free radicles and reactive oxygen species. The functional properties of LMPs greatly depends on degree of methylation, source, structure, and sugar composition. Recently, LMPs are used in food industry as emulsifying, gelling, flocculation and foaming agent. This paper highlights the recent technological advancements in production, and functional properties of LMPs derived from pectin rich agro waste. In future, LMP based nutraceutical products can be developed by pharma and food industry.

全球人口的增加导致新疾病的大量增加，带来了新的挑战。目前使用的治疗方法是非特异性的，并且有一些副作用。迫切需要找到替代的、安全的预防和治疗战略来防治各种疾病。面对粮食不安全、慢性病增加和追求可持续经济做法等全球挑战，对从植物废料中提取的膳食纤维或营养保健品的需求日益重要。近年来，从富含果胶的农业废弃物中提取的低甲基化果胶（LMP）在预防和治疗疾病方面具有良好的功能特性。LMPs通常被认为是安全、无毒和生物相容性好的。据报道，LMPs与半乳糖素-3受体结合可预防肿瘤转移，通过抑制α-葡萄糖苷酶活性发挥抗糖尿病作用，通过调节TLR-2、IL-1β和IL-6促进抗炎活性。LMPs通过促进有益细菌的生长，通过产生短链脂肪酸来中和毒素，从而表现出抗菌活性。LMPs通过减少自由基和活性氧来促进抗氧化活性。LMPs的功能特性在很大程度上取决于甲基化程度、来源、结构和糖组成。近年来，LMPs作为乳化剂、胶凝剂、絮凝剂和发泡剂在食品工业中得到广泛应用。本文重点介绍了从富含果胶的农业废弃物中提取LMPs的最新技术进展和功能特性。未来，基于LMP的营养保健品可用于制药和食品工业。

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引用次数: 0

Pectic polysaccharides from Açaí (Euterpe oleracea) show antitumoral effects on melanoma cell line 来自Açaí （Euterpe oleracea）的果胶多糖显示出对黑色素瘤细胞系的抗肿瘤作用

IF 3.6

Bioactive Carbohydrates and Dietary Fibre

Pub Date : 2025-12-31 DOI: 10.1016/j.bcdf.2025.100520

Suelen Cristina Soares Baal , Amanda Plaça Bialli , Rafaella Fernanda Angeli , Luiz Claudio Fernandes , Katya Naliwaiko , Lucimara Mach Côrtes Cordeiro , Marcia Helena Appel , Fabíola Iagher

Brazil has a rich biodiversity of natural compounds with high biotechnological potential that can play an essential role in preventing and treating diseases, including cancer. Açaí (Euterpe oleracea), a fruit native to Brazil, is known for its notable biological properties, primarily attributed to its phenolic compounds. However, despite the recognized biological activities of pectic polysaccharides from various fruits, those derived from açaí remain largely unexplored. This research evaluated the in vitro antitumor activity of a pectic polysaccharide fraction obtained from the açaí pulp (APF) on the murine melanoma B16F10 cell line. According to the monosaccharide composition analysis, gel permeation chromatography analysis and ¹H/¹³C HSQC-DEPT correlation map, APF is composed of type II arabinogalactan, homogalacturonan and small amounts of type I rhamnogalacturonan. The cytotoxicity of the polysaccharides was evaluated on normal BALB/c 3T3 murine fibroblast cells. Treatment with the APF fraction at concentrations of 5 and 10 μg/mL exhibited cytotoxic effects against the tumor cell line, without affecting the viability or inducing cytotoxicity in normal cells. In addition, treatment with APF at concentrations of 5 and 10 μg/mL reduced the proliferation of melanoma cells and promoted apoptosis. At 10 μg/mL, APF significantly decreased the clonogenic capacity of the tumor cell line, as indicated by a reduction in the average number of colonies, and induced cell cycle arrest. The fraction had no effect on the migratory capacity of the tumor cells. These findings highlight the promising antitumor potential of the pectic polysaccharide fraction derived from açaí fruit.

巴西拥有丰富的天然化合物生物多样性，具有很高的生物技术潜力，可在预防和治疗包括癌症在内的疾病方面发挥重要作用。Açaí （Euterpe oleracea）是一种原产于巴西的水果，以其显著的生物学特性而闻名，主要归因于其酚类化合物。然而，尽管从各种水果中提取的果胶多糖具有公认的生物活性，但从açaí提取的果胶多糖在很大程度上仍未被开发。本研究评价了从açaí果肉中提取的果胶多糖（APF）对小鼠黑色素瘤B16F10细胞系的体外抗肿瘤活性。根据单糖组成分析、凝胶渗透色谱分析和1H/13C HSQC-DEPT相关图可知，APF由II型阿拉伯半乳糖酸、均型半乳糖酸和少量I型鼠李糖半乳糖酸组成。对正常BALB/c 3T3小鼠成纤维细胞进行了细胞毒性评价。APF组分浓度为5和10 μg/mL时，对肿瘤细胞系具有细胞毒作用，不影响正常细胞的活性，也不诱导细胞毒性。此外，浓度为5和10 μg/mL的APF可抑制黑色素瘤细胞的增殖，促进细胞凋亡。在10 μg/mL浓度下，APF显著降低肿瘤细胞系的克隆生成能力，平均菌落数减少，并诱导细胞周期阻滞。对肿瘤细胞的迁移能力无影响。这些发现突出了从açaí水果中提取的果胶多糖的抗肿瘤潜力。

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引用次数: 0

Evaluation of the Relative Ordered Structure of Polysaccharide: a case study on polysaccharide characterization from Gymnadenia conopsea 多糖的相对有序结构评价——以金针菇多糖表征为例

IF 3.6

Bioactive Carbohydrates and Dietary Fibre

Pub Date : 2025-12-27 DOI: 10.1016/j.bcdf.2025.100519

Junqiao Wang, Chengxi Feng, Hui Yan, Zhe Wang, Yajie Wang, Junyi Yin, Shaoping Nie

Polysaccharide, one of the major bioactive components in food materials, is hard to be characterized duo to complex structures. Previous theoretical framework of “relative ordered structure of the polysaccharide” has been well-documented in several mushroom polysaccharides but have not been demonstrated in plant bioactive polysaccharides. This study utilized this theory to guide fractionation and characterization of polysaccharide from tubes of Gymnadenia conopsea (G. conopsea). Two polysaccharides were extracted and characterized with regard to molecular weight, monosaccharide composition, substitution position, linkage patterns, and sequence. Results showed that two polysaccharide fractions showed distinctive structures. One was an O-acetyl-glucomanan containing →4)-β-Manp-(1→ and →4)-β-Glcp-(1→ as main chain structure and mono-substituted with acetyl groups at O-2, O-3 or O-6 position. The other was an α-glucan comprising backbone structure of →4)-α-Glcp-(1→ with branch substituted at C-6 and C-3 and α-Glcp-(1→ as the side chains. Altogether, this findings enrich the definition of “relative ordered structure of the polysaccharide” regarding plant polysaccharide, and was also the first study to fully understand the chemistry of such bioactive polysaccharides in G. conopsea.

多糖是食品原料中主要的生物活性成分之一，由于其结构复杂，难以对其进行表征。先前的“多糖的相对有序结构”理论框架已在几种蘑菇多糖中得到了充分的证明，但尚未在植物生物活性多糖中得到证实。本研究利用这一理论指导荆芥多糖的分离和表征。提取了两种多糖，并对其分子量、单糖组成、取代位置、连锁模式和序列进行了表征。结果表明，两种多糖组分具有不同的结构。一种是o-乙酰基葡甘聚糖，主链结构为→4)-β- manp -(1→和→4)-β- glcp -(1→，并在O-2、O-3或O-6位置被乙酰基单取代。另一种是α-葡聚糖，其主链结构为→4)-α-Glcp-(1)→，支链取代于C-6和C-3， α-Glcp-(1)→为侧链。本研究丰富了植物多糖中“多糖的相对有序结构”的定义，也是首次全面了解蛇麻属生物活性多糖化学性质的研究。

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引用次数: 0

Molecular docking and GC-MS profiling of seaweed bioactive components: Unraveling antibacterial and antibiofilm activity in carbohydrate-rich extracts 海藻生物活性成分的分子对接和GC-MS分析：揭示富含碳水化合物提取物的抗菌和抗生物膜活性

IF 3.6

Bioactive Carbohydrates and Dietary Fibre

Pub Date : 2025-12-11 DOI: 10.1016/j.bcdf.2025.100516

Eman A. Alwaleed, Aya A. Alrashedi, Asmaa S. Abdelgeliel

In this study, the antibacterial and antibiofilm activities of ethanolic and methanolic extracts of seaweed species, Corallina officinalis, Padina pavonica, and Caulerpa lentillifera, were systematically evaluated. Eleven clinically resistant bacterial strains, representing both Gram-positive and Gram-negative species, were tested to assess the broad-spectrum efficacy of the extracts. Phytochemical characterization using GC–MS and FTIR analyses revealed several bioactive compounds that may be responsible for the observed antimicrobial effects, including significant inhibition of biofilm formation. Among the tested samples, the methanolic extract of P. pavonica exhibited the most potent antibacterial activity, with a minimum inhibitory concentration (MIC) of 70 mg/mL against Streptococcus pyogenes and a minimum bactericidal concentration (MBC) of 160 mg/mL against Bacillus velezensis. These findings suggest that marine algae represent a valuable source of novel antimicrobial agents with potential pharmaceutical and medicinal applications. Furthermore, molecular docking results provide additional insight into the possible mechanisms of action, supporting the development of seaweed-derived compounds as promising candidates for future therapeutic interventions.

本研究系统评价了海苔（Corallina officinalis）、帕迪纳（Padina pavonica）和小扁豆（Caulerpa lentillifera）的乙醇和甲醇提取物的抗菌和抗膜活性。11种临床耐药菌株，分别代表革兰氏阳性和革兰氏阴性菌株，进行了测试，以评估提取物的广谱疗效。利用GC-MS和FTIR分析的植物化学特征揭示了几种生物活性化合物可能负责观察到的抗菌作用，包括显著抑制生物膜的形成。其中，金银花甲醇提取物的抑菌活性最强，对化脓性链球菌的最低抑菌浓度为70 mg/mL，对velezensis芽孢杆菌的最低杀菌浓度为160 mg/mL。这些发现表明，海藻是具有潜在制药和医疗应用价值的新型抗菌剂的宝贵来源。此外，分子对接结果为可能的作用机制提供了额外的见解，支持了海藻衍生化合物作为未来治疗干预措施的有希望的候选者的发展。

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引用次数: 0

Aims and Scope & Editorial Board 目标和范围&编委会

IF 3.6

Bioactive Carbohydrates and Dietary Fibre

Pub Date : 2025-12-01 DOI: 10.1016/S2212-6198(25)00051-8

引用次数: 0

In vitro antioxidant properties of modified cassava flour and its untargeted metabolomics 改性木薯粉的体外抗氧化性能及其非靶向代谢组学研究

IF 3.6

Bioactive Carbohydrates and Dietary Fibre

Pub Date : 2025-12-01 DOI: 10.1016/j.bcdf.2025.100513

Yuniar Khasanah , Anastasia Wheni Indrianingsih , Agnes Murdiati , Priyanto Triwitono

The prevalence of diabetes increases. encouraging the utilization of biological resources to the greatest extent possible. Because of its bioactive components. yellow-fleshed cassava is regarded as a functional food source as well as a potential antioxidant. The purpose of this study was to evaluate the antioxidant properties of modified cassava flour. The antioxidant properties of the extracts and fractions were assessed using the 2.2-diphenyl-1-picryl-hydrazyl assay (DPPH). 2,2-azinobis (3-ethyl-benzothiazoline-6-sulfonic acid (ABTS). β-carotene bleaching assay. and Ferric Reducing Antioxidant Power (FRAP). Total phenolic content (TPC) and bioactive compounds were studied. The results showed TPC of modified cassava flour ranged from 5.46 mg GAE/g – 25.83 mg GAE/g. antioxidant activity using DPPH assay. ABTS assay. FRAP assay and β-carotene bleaching were 7.59 %–32.55 %; 17.91 mg TE/g – 45.22 mg TE/g; 0.05 mmol Fe (II)/g – 0.51 mmol Fe (II)/g; and 60.29 %–76.71 % respectively. Untargeted metabolomic using Liquid Chromatography – High Resolution Mass Spectrometry (LC-HRMS) showed that some bioactive compounds have the potential to act as antioxidants. such as 2-Anisic acid, 7-hydrohy-6-methoxy-2H-chromen-2-one (Scopoletin). 7-methoxy-6-{[(2S,3R,4S,5S,6R)-3,4,5-trihydroxy-6-(hydroxymethyl)oxan-2-yl]oxy}-2H-chromen-2-one (scopoline), Norharman, α,α-Trehalose,and α-Eleostearic acid.

糖尿病患病率上升。鼓励最大限度地利用生物资源。因为它的生物活性成分。黄瓤木薯被认为是一种功能性食物来源，也是一种潜在的抗氧化剂。本研究的目的是评价改性木薯粉的抗氧化性能。采用2.2-diphenyl-1-picryl-hydrazyl法（DPPH）评价提取物和馏分的抗氧化性能。2,2-氮化萘（3-乙基-苯并噻唑啉-6-磺酸）β-胡萝卜素漂白试验。和铁还原抗氧化能力（FRAP）。研究了总酚含量（TPC）和生物活性成分。结果表明，改性木薯粉的TPC在5.46 mg GAE/g ~ 25.83 mg GAE/g之间。DPPH法测定抗氧化活性。abt化验。FRAP法和β-胡萝卜素漂白法分别为7.59% ~ 32.55%；17.91 mg TE/g - 45.22 mg TE/g；0.05 mmol Fe (II)/g - 0.51 mmol Fe (II)/g；60.29% ~ 76.71%。利用液相色谱-高分辨率质谱（LC-HRMS）进行的非靶向代谢组学研究表明，一些生物活性化合物具有抗氧化剂的潜力。如2-茴香酸，7-羟基-6-甲氧基- 2h - chromen2 -one（东莨菪碱）。7-甲氧基-6-{[(2S,3R,4S,5S,6R)-3,4,5-三羟基-6-（羟甲基）氧-2-基]氧}- 2h - chromenon -2-one（东莨菪碱），Norharman, α，α-海藻糖，α-骨脂酸。

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引用次数: 0

Corrigendum to “Dietary bioactive compounds poly-β-hydroxybutyrate, mannan oligosaccharide, and inulin affected the growth performance, gut microbiota, and body composition of African catfish (Clarias gariepinus, Burchell, 1822) larvae” [Bioactive Carbohydrates and Dietary Fibre 34 (2025) 1-1 100501] “膳食生物活性化合物聚β-羟基丁酸盐、甘露聚糖低聚糖和菊粉影响非洲鲶鱼（Clarias gariepinus, Burchell, 1822）幼虫的生长性能、肠道微生物群和体成分”的更正[生物活性碳水化合物和膳食纤维34 (2025)1-1 100501]

IF 3.6

Bioactive Carbohydrates and Dietary Fibre

Pub Date : 2025-12-01 DOI: 10.1016/j.bcdf.2025.100506

Solomon Melaku , Abebe Getahun , Seyoum Mengestou , Akewake Geremew , Amha Belay

引用次数: 0

Regulating IL-17-NFκB signaling and gut microbiota in Alzheimer's disease: Mechanism of forsythoside A and Lonicera japonica polysaccharide combination 阿尔茨海默病中调节IL-17-NFκB信号和肠道微生物群：连翘苷A和忍冬多糖联合作用的机制

IF 3.6

Bioactive Carbohydrates and Dietary Fibre

Pub Date : 2025-12-01 DOI: 10.1016/j.bcdf.2025.100514

Yanxia Shu , Ke Li , Zongshuo Li , Weiran Feng , Weidong Li

This study investigated the combined therapeutic effects of Forsythoside A (FA) and Lonicera japonica polysaccharide (LP) on Alzheimer's disease (AD) through dual modulation of neuroinflammation and gut microbiota. HT22 cells were treated with Aβ_25-35 to establish an AD model for examining the synergistic concentration ratio of FA and LP. An APP/PS1 double-transgenic AD mouse model was developed. The anti-AD activities of FA + LP were evaluated through behavioral testing, measuring amyloid-beta (Aβ) and phosphorylated Tau (p-Tau) protein levels in the hippocampus and cortex, and assessing serum inflammatory factors. Transcriptomic analysis, coupled with quantitative reverse transcription-polymerase chain reaction (qRT-PCR) and western blotting, was used to validate the molecular mechanism by which FA + LP improved AD pathology in mice. Finally, 16S rRNA sequencing technology was used to explore the anti-AD mechanisms of FA + LP from a gut microecology perspective. We found the optimal FA:LP synergistic ratio was 1:5. FA + LP treatment significantly improved cognitive function in behavioral tests while reducing Aβ and p-Tau accumulation in the hippocampus and cortex. Serum analysis showed decreased levels of pro-inflammatory cytokines (TNF-α, IL-1β, IL-6). Mechanistic studies revealed FA + LP downregulated the IL-17-NFκB pathway, evidenced by reduced mRNA expression of Il-17a, Act1, Iκba, Nfκb p65, and Cox2, along with decreased IL-17A and NFκB p65 protein levels. Gut microbiota analysis demonstrated FA + LP increased beneficial bacteria (Lachnospiraceae and Muribaculum) while normalizing the Firmicutes/Bacteroidetes ratio. The combination therapy outperformed single treatments across all evaluated parameters. Our results demonstrate FA + LP (1:5) alleviates AD pathology through: suppressing neuroinflammation via IL-17-NFκB signaling inhibition, and restoring gut microbiota balance.

本研究探讨连翘苷A （FA）和忍冬多糖（LP）通过双重调节神经炎症和肠道菌群对阿尔茨海默病（AD）的联合治疗作用。采用a - β25-35处理HT22细胞，建立AD模型，检测FA与LP的协同浓度比。建立APP/PS1双转基因AD小鼠模型。通过行为学测试、海马和皮层淀粉样蛋白（Aβ）和磷酸化Tau蛋白（p-Tau）水平以及血清炎症因子评估FA + LP抗ad活性。通过转录组学分析，结合定量逆转录聚合酶链反应（qRT-PCR）和western blotting，验证FA + LP改善小鼠AD病理的分子机制。最后，利用16S rRNA测序技术从肠道微生态学角度探讨FA + LP抗ad的机制。我们发现最佳的FA:LP增效比为1:5。FA + LP治疗可显著改善行为测试中的认知功能，同时减少海马和皮层中Aβ和p-Tau的积累。血清分析显示，促炎细胞因子（TNF-α、IL-1β、IL-6）水平降低。机制研究显示FA + LP下调IL-17-NFκB通路，表现为Il-17a、Act1、i - κba、nf - κb p65、Cox2 mRNA表达降低，Il-17a、nf - κb p65蛋白水平降低。肠道菌群分析表明，FA + LP增加了有益细菌（毛缕菌科和Muribaculum），同时使厚壁菌门/拟杆菌门比例正常化。在所有评估参数中，联合治疗优于单一治疗。我们的研究结果表明，FA + LP（1:5）通过抑制IL-17-NFκB信号传导抑制神经炎症，恢复肠道菌群平衡，从而减轻AD病理。

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引用次数: 0