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Enzymatic modification of wheat bran: Effect on functional and physicochemical properties 小麦麸皮的酶修饰:对其功能和理化性质的影响
Pub Date : 2025-02-21 DOI: 10.1016/j.bcdf.2025.100472
Reshma Saroj , Mudasir Ahmad Malik , Devinder Kaur
Bran from six various wheat varieties was used in this study subjected to enzymatic treatment and was evaluated for physicochemical, functional and antioxidant properties along with mineral content analysis. Six wheat cultivars used for the study were HUW-234, PBW-373, WH-1105, PBW-502, HD-2967, and PBW-343. Wheat bran was treated with three different enzymes namely β-gluconase, Xylanase and Cellulase. The functional properties of bran were substantially altered by the enzyme treatment. All enzyme treatments caused increase in bulk density and decrease in water binding capacity, oil binding capacity and swelling power. Maximum effect on functional properties was observed by the cellulase enzyme modification. Total phenol content and antioxidant properties were increased after enzyme treatment and maximum increase was found in cellulase enzyme treatment followed by xylanase and β-gluconase. Soluble fiber content was increased while as insoluble fiber content was decrease after enzyme modification. The concentration of calcium was found highest and concentration of cupper was found lowest in all bran samples and concentration of all the studied minerals increased significantly (p ≤ 0.05) after enzyme treatment. Pearson correlation and he principal component analysis was carried out to determine the relationship among different physicochemical, functional and antioxidant properties of bran samples.
本研究采用6种不同小麦品种的麸皮进行酶处理,并对其理化、功能和抗氧化性能以及矿物质含量进行了评价。6个小麦品种分别为HUW-234、PBW-373、WH-1105、PBW-502、HD-2967和PBW-343。用β-葡萄糖酶、木聚糖酶和纤维素酶对麦麸进行处理。经酶处理后,麸皮的功能特性发生了很大的变化。各酶处理均使其容重增加,水结合力、油结合力和膨胀力降低。纤维素酶修饰对其功能性能的影响最大。酶处理后总酚含量和抗氧化性能均有所提高,以纤维素酶处理增幅最大,其次是木聚糖酶和β-葡萄糖酶。经酶修饰后,可溶性纤维含量增加,不溶性纤维含量降低。酶处理后,各麸皮样品中钙的浓度最高,铜的浓度最低,各矿物质的浓度均显著升高(p≤0.05)。通过Pearson相关和主成分分析,确定了麸皮样品的理化、功能和抗氧化性能之间的关系。
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引用次数: 0
Antitumor activity of polysaccharides obtained from guavira fruit industrial waste on murine melanoma cells 番石榴果工业废料多糖对小鼠黑色素瘤细胞的抗肿瘤活性
Pub Date : 2025-01-31 DOI: 10.1016/j.bcdf.2025.100469
Rafaella Fernanda Angeli , Amanda Plaça Bialli , Suelen Cristina Soares Baal , Elisvânia Freitas dos Santos , Paulo Sérgio Loubet Filho , Vanessa Suzane Schneider , Luiz Cláudio Fernandes , Katya Naliwaiko , Lucimara Mach Côrtes Cordeiro , Fabíola Iagher
Melanoma is an aggressive type of cancer with increasing incidence worldwide. Chemotherapy drugs are associated with several side effects that lead to lost quality of life for patients undergoing treatment. This fact has encouraged the search for compounds capable of acting as adjuvants in cancer treatment with fewer side effects. Pectins are soluble polysaccharides with diverse molecular structures and important biological properties, which can be obtained from fruit parts often discarded by the food industry. The present study investigated the antitumor effect of pectins present in guavira pomace flour (Campomanesia adamantium and C. pubescens) on B16F10 cells. The assays evaluated viability, cell death, proliferation rate, migratory ability, and potential for colony formation. The results showed that pectins (CPW fraction) at concentrations of 5 and 100 μg/mL reduced the viability of tumor cells (∼20–40%, respectively, when compared to the untreated group), with less cytotoxicity on non-tumor cells (BALB/c 3T3) than the chemotherapy drug Doxorubicin (p < 0.05). Similarly, the proliferation rate was decreased in B16F10 cells in the presence of two CPW concentrations (∼33 and 40%, respectively). In the flow cytometry assay, the CPW treatment did not increase the number of dead cells, suggesting cell cycle arrest. In addition, the CPW fraction reduced the migratory capacity of tumor cells (∼8 and 12%, respectively) and the number of new colonies formed (∼30 and 70%, respectively). Taken together, these results suggest that the pectins of guavira pomace flour, a discarded industrial material, have important antitumor activity with adjuvant therapeutic potential for cancer treatment.
黑色素瘤是一种侵袭性癌症,在世界范围内发病率不断上升。化疗药物与一些副作用有关,这些副作用会导致接受治疗的患者生活质量下降。这一事实鼓励人们寻找能够在癌症治疗中作为佐剂而副作用更小的化合物。果胶是一种具有多种分子结构和重要生物学特性的可溶性多糖,可从食品工业经常丢弃的水果部分中提取。研究番石榴果渣粉中果胶对B16F10细胞的抗肿瘤作用。这些试验评估了细胞活力、细胞死亡、增殖率、迁移能力和集落形成的潜力。结果表明,浓度为5和100 μg/mL的果胶(CPW部分)与未处理组相比,分别降低了肿瘤细胞的活力(约20-40%),对非肿瘤细胞(BALB/c 3T3)的细胞毒性低于化疗药物阿霉素(p <;0.05)。同样,在两种CPW浓度(分别为~ 33和40%)存在下,B16F10细胞的增殖率降低。在流式细胞术检测中,CPW处理没有增加死亡细胞的数量,提示细胞周期阻滞。此外,CPW组分降低了肿瘤细胞的迁移能力(分别为~ 8%和12%)和形成的新菌落数量(分别为~ 30%和70%)。综上所述,番石榴果渣粉作为一种废弃的工业原料,其果胶具有重要的抗肿瘤活性,具有辅助治疗癌症的潜力。
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引用次数: 0
Synthesis of sulfated chitosan from cuttlebone of Sepia pharaonis (Ehrenberg, 1831) and its capacity to inhibit blood clotting 从法老乌贼骨中合成硫酸壳聚糖(Ehrenberg, 1831)及其抑制血液凝固的能力
Pub Date : 2025-01-28 DOI: 10.1016/j.bcdf.2025.100470
Hirshasri Ambalarajan Gunasekaran , Annathai Pitchai , Pasiyappazham Ramasamy
The synthesis of sulfated chitosan from the cuttlebone of Sepia pharaonis (Ehrenberg, 1831) and its potential to inhibit blood clotting were investigated in this study. Cuttlebone-derived chitosan was chemically modified by sulfation to produce sulfated chitosan. This modification was achieved by introducing sulfate groups into the chitosan structure, thereby enhancing its biological properties. The synthesized sulfated chitosan demonstrated a significant capacity to inhibit blood clotting, indicating its potential application as an anticoagulant. Anticoagulant activity was evaluated through in vitro assays, where sulfated chitosan exhibited notable suppression of clot formation. Moreover, this study examined the structural and physicochemical properties (FTIR, XRD, FESEM) of the synthesized sulfated chitosan, providing insights into its potential mechanism of action. These findings suggest that sulfated chitosan derived from Sepia pharaonis cuttlebone holds promise as an effective and biocompatible anticoagulant, thereby presenting a potential avenue for the development of novel therapeutic interventions in the field of cardiovascular medicine and related disciplines.
本研究探讨了从法老乌贼(Sepia pharaonis, Ehrenberg, 1831)的乌贼骨中合成硫酸化壳聚糖及其抑制血液凝固的潜力。对海螵蛸壳聚糖进行了磺化改性,制备了磺化壳聚糖。这种改性是通过在壳聚糖结构中引入硫酸盐基团来实现的,从而提高了壳聚糖的生物学性能。合成的硫酸壳聚糖具有明显的抗凝血能力,表明其作为抗凝血剂的潜在应用前景。通过体外试验评估抗凝血活性,硫酸化壳聚糖表现出明显的凝块形成抑制作用。此外,本研究还对合成的硫酸壳聚糖的结构和理化性质(FTIR, XRD, FESEM)进行了测试,为其潜在的作用机制提供了新的见解。这些发现表明,从法老乌贼鱼骨中提取的硫酸化壳聚糖有望成为一种有效的生物相容性抗凝剂,从而为心血管医学和相关学科领域的新型治疗干预提供了潜在的途径。
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引用次数: 0
Livaux® gold kiwifruit powder consumption at 600 mg per day for 28 days increases Faecalibacterium prausnitzii numbers and decreases bloating and hydrogenotrophic species numbers in healthy individuals, consistent with slow fermentation: A randomized controlled trial 一项随机对照试验:每天摄入600毫克Livaux®金猕猴桃粉,持续28天,会增加健康个体的prausnitzii粪杆菌数量,减少腹胀和氢营养物种数量,与缓慢发酵一致
Pub Date : 2025-01-17 DOI: 10.1016/j.bcdf.2025.100468
Starin McKeen , Susanne M. Henning , Erin Lewis , Malkanthi Evans , Emma Graham , Neville Jopson , Jennifer Gu , Doug Rosendale
Kiwifruit is renowned for its benefits to laxation. Livaux® is a gold kiwifruit powder retaining whole fruit benefits, including high-methoxy pectin content. Livaux was previously shown to provide laxation support and increased faecal F. prausnitzii. F. prausnitzii is known to use high-methoxy pectin as substrate.
We aimed to determine the changes to laxation and faecal F. prausnitzii numbers from 600 mg of Livaux. We used a randomized, double-blinded, placebo-controlled parallel-arm study with 85 participants conducted across four North American sites, comparing 28 days of 600 mg Livaux consumption vs cellulose placebo. The study was powered for the primary outcome Complete Spontaneous Bowel Movements (CSBM).
Participants visited at screening, baseline and endpoint. Anthropometric measures, hematology, clinical chemistry and hematology and fecal samples for microbiome assessment were collected at baseline and endpoint. Clinical outcomes (CSBM, Bristol Stool Form Score (BSFS), spontaneous bowel movements (SBM) and participant-assessed constipation symptoms and quality of life), food diaries and adverse events were recorded daily throughout the study. Analysis of the intent-to-treat population assessed differences from baseline to endpoint using ANOVA with post-hoc Tukey's T-test, an assessment of change from baseline to days 7, 14, 21 and 28 using repeated measures ANCOVA.
Livaux supplemented participants showed significantly increased fecal F. prausnitzii relative abundance, whilst placebo did not. Participants also showed significantly improved CSBM, SBM, BSFS and constipation symptom and quality of life scores over baseline.
Livaux supplementation improved laxation and increased F. prausnitzii at the low dose of 600 mg.
This study was registered at https://clinicaltrials.gov/study/NCT03462199.
猕猴桃以其通便功效而闻名。Livaux®是一种黄金猕猴桃粉,保留了整个水果的好处,包括高甲氧基果胶含量。Livaux先前被证明可以提供通便支持和增加粪F. prausnitzii。已知prausnitzii以高甲氧基果胶为底物。我们的目的是确定600毫克利沃克斯对通便和粪便中prausnitzi数量的变化。我们采用了一项随机、双盲、安慰剂对照的平行研究,在北美的四个地点进行了85名参与者,比较了28天600毫克Livaux和纤维素安慰剂的摄入量。该研究的主要结果是完全自发排便(CSBM)。参与者在筛查、基线和终点进行了访问。在基线和终点收集人体测量、血液学、临床化学、血液学和粪便样本,用于微生物组评估。在整个研究过程中,每天记录临床结果(CSBM、Bristol大便形式评分(BSFS)、自发排便(SBM)和参与者评估的便秘症状和生活质量)、食物日记和不良事件。使用方差分析和事后Tukey’st检验评估治疗意向人群从基线到终点的差异,使用重复测量ANCOVA评估从基线到第7、14、21和28天的变化。补充Livaux的参与者粪便中F. prausnitzii的相对丰度显著增加,而安慰剂则没有。与基线相比,参与者的CSBM、SBM、BSFS、便秘症状和生活质量评分也有显著改善。在低剂量600 mg时,补充Livaux可改善通便并增加prausnitzi。本研究注册于https://clinicaltrials.gov/study/NCT03462199。
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引用次数: 0
Physicochemical characterization of starch from Maranta arundinacea L. (arrowroot) rhizomes and its inhibition of COX-2: In vivo validation 竹芋根茎淀粉的理化性质及其对COX-2的抑制作用:体内验证
Pub Date : 2025-01-07 DOI: 10.1016/j.bcdf.2024.100465
Nishaa Surendran , Manikandan Vani Raju , Meenakshi Kaniyur Chandrasekaran , Rathi Muthaiyan Ahalliya , Chella Perumal Palanisamy , Geethadevi Chandrasekaran , Gopalakrishnan Velliyur Kanniappan
Maranta arundinacea L. (M. arundinacea L.) that belongs to the family Marantaceae is an underutilized tuber. The starch from the rhizomatous tubers is highly commercial and medicinal value is uncharacterized. This study was aimed at isolating the starch from Maranta arundinacea L. rhizomes, analyzing its physiochemical properties and examine its influence on anti-inflammatory activity. The physicochemical properties like Moisture and ash content, water holding capacity, water absorption index, amylose content, light transmittance and structural morphology of starch granules. Anti-inflammatory activity of starch from M. arundinacea L. was evaluated in Wistar rats by carrageenan induced paw edema. The biochemical estimation of serum enzymes, lipid peroxidation, enzymatic antioxidants and histopathological studies of paw tissues were carried out in paw tissues. Molecular expression of proinflammatory COX-2 gene was also analyzed. M. arundinacea starch was found to contain 9.3% moisture, 1.36% ash and 26.3% amylose content. The light transmittance (%) decreased with an increase in time. Micrographs of SEM analysis showed variations in the shape of granules that appeared as spherical and irregular. The size of granules varied between 2.86 and 6.92 μm. Attenuation in paw edema on treatment with M. arundinacea L. starch (200 mg/kg) was observed in comparison with that of standard drug Diclofenac sodium, the serum enzymes and enzymatic antioxidants were brought to near normal. The potent anti-inflammatory activities were confirmed by Histopathological studies. Molecular expression of proinflammatory COX-2 gene was also down regulated. It can be concluded that the dietary supplementation of starch from M. arundinacea L. to mitigate inflammation and reduce chronic diseases.
马兰属马兰科,是一种未被充分利用的块茎植物。从根茎块茎中提取的淀粉具有很高的商业价值,药用价值尚不明确。本研究旨在从马兰花根茎中分离淀粉,分析其理化性质,并考察其抗炎活性的影响。研究了淀粉颗粒的水分、灰分、持水量、吸水指数、直链淀粉含量、透光率、结构形态等理化性质。用角叉菜胶诱导Wistar大鼠足跖水肿的方法,评价了黄芪淀粉的抗炎活性。在足跖组织中进行血清酶生化测定、脂质过氧化、酶促抗氧化剂测定和组织病理学研究。分析促炎COX-2基因的分子表达。其淀粉含水量为9.3%,灰分含量为1.36%,直链淀粉含量为26.3%。透光率(%)随时间的增加而降低。扫描电镜分析显示颗粒形状的变化,表现为球形和不规则。颗粒大小在2.86 ~ 6.92 μm之间。与标准药双氯芬酸钠相比,200 mg/kg的黄芪淀粉对大鼠足部水肿有明显的缓解作用,血清酶和酶抗氧化剂恢复到接近正常水平。组织病理学研究证实其有效的抗炎活性。促炎COX-2基因的分子表达也下调。由此可见,饲粮中添加太子参淀粉具有减轻炎症和减少慢性疾病的作用。
{"title":"Physicochemical characterization of starch from Maranta arundinacea L. (arrowroot) rhizomes and its inhibition of COX-2: In vivo validation","authors":"Nishaa Surendran ,&nbsp;Manikandan Vani Raju ,&nbsp;Meenakshi Kaniyur Chandrasekaran ,&nbsp;Rathi Muthaiyan Ahalliya ,&nbsp;Chella Perumal Palanisamy ,&nbsp;Geethadevi Chandrasekaran ,&nbsp;Gopalakrishnan Velliyur Kanniappan","doi":"10.1016/j.bcdf.2024.100465","DOIUrl":"10.1016/j.bcdf.2024.100465","url":null,"abstract":"<div><div><em>Maranta arundinacea</em> L. (<em>M. arundinacea</em> L.) that belongs to the family Marantaceae is an underutilized tuber. The starch from the rhizomatous tubers is highly commercial and medicinal value is uncharacterized. This study was aimed at isolating the starch from <em>Maranta arundinacea</em> L. rhizomes, analyzing its physiochemical properties and examine its influence on anti-inflammatory activity. The physicochemical properties like Moisture and ash content, water holding capacity, water absorption index, amylose content, light transmittance and structural morphology of starch granules. Anti-inflammatory activity of starch from <em>M. arundinacea</em> L. was evaluated in Wistar rats by carrageenan induced paw edema. The biochemical estimation of serum enzymes, lipid peroxidation, enzymatic antioxidants and histopathological studies of paw tissues were carried out in paw tissues. Molecular expression of proinflammatory COX-2 gene was also analyzed. <em>M. arundinacea</em> starch was found to contain 9.3% moisture, 1.36% ash and 26.3% amylose content. The light transmittance (%) decreased with an increase in time. Micrographs of SEM analysis showed variations in the shape of granules that appeared as spherical and irregular. The size of granules varied between 2.86 and 6.92 μm. Attenuation in paw edema on treatment with <em>M. arundinacea</em> L. starch (200 mg/kg) was observed in comparison with that of standard drug Diclofenac sodium, the serum enzymes and enzymatic antioxidants were brought to near normal. The potent anti-inflammatory activities were confirmed by Histopathological studies. Molecular expression of proinflammatory COX-2 gene was also down regulated. It can be concluded that the dietary supplementation of starch from <em>M. arundinacea</em> L. to mitigate inflammation and reduce chronic diseases.</div></div>","PeriodicalId":38299,"journal":{"name":"Bioactive Carbohydrates and Dietary Fibre","volume":"33 ","pages":"Article 100465"},"PeriodicalIF":0.0,"publicationDate":"2025-01-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143147341","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Sorghum cookies fortified with Garcinia mangostana peel extract: Formulation, characterization, and evaluation of antioxidant and antidiabetic activity 山竹果皮提取物强化高粱饼干:配方、特性和抗氧化和抗糖尿病活性的评价
Pub Date : 2025-01-04 DOI: 10.1016/j.bcdf.2025.100467
Anastasia Wheni Indrianingsih , Yuniar Khasanah , Cici Darsih , Heru Pitria Hastuti , Ade Erma Suryani , Margi Hastuti , Dwi Ni'maturrohmah , Umi Laila , Eka Noviana , Eka Rahayu , Tri Wiyono
Increased consumption of processed food and sugary beverages is a major contributor to chronic diseases such as diabetes mellitus and cardiovascular diseases. This research aimed to develop sorghum-based cookie fortified with mangosteen (Garcinia mangostana) peel extract, as a healthy, yet convenient food alternative. Both sorghum and mangosteen are rich in phenolic compounds that have been shown to possess several biological activities including antioxidant and antidiabetic activities. The cookies were evaluated physically and chemically by performing sensory, color, texture, proximate, and chemical content analysis, determination of total phenolic content, and further characterizations using scanning electron microscopy (SEM), X-ray diffraction (XRD), X-ray fluorescence spectroscopy (XRF), and Fourier transform infrared spectroscopy (FTIR). The antioxidant and antidiabetic potencies of the cookies were assessed using radical scavenging and α-glucosidase inhibitory assays, respectively. The results showed that substituting wheat with sorghum flour and adding mangosteen peel extract to the cookie ingredients increased its antioxidant and antidiabetic activity. Several chemical compounds detected from the sorghum cookies included xanthones, phenolics, flavonoids, and alkaloids. The mangosteen peel extract-fortified sorghum cookies were well accepted by the panelists based on sensory and hedonic testing. Hence, sorghum flour and mangosteen peel can be used as excellent ingredients for making healthy cookies.
加工食品和含糖饮料消费量的增加是糖尿病和心血管疾病等慢性疾病的主要原因。本研究旨在开发以山竹果皮提取物强化的高粱饼干,作为一种健康、方便的食品替代品。高粱和山竹都富含酚类化合物,已被证明具有多种生物活性,包括抗氧化和抗糖尿病活性。通过感官、颜色、质地、近似和化学含量分析、总酚含量测定,以及扫描电子显微镜(SEM)、x射线衍射(XRD)、x射线荧光光谱(XRF)和傅里叶变换红外光谱(FTIR)的进一步表征,对饼干进行了物理和化学评价。通过自由基清除和α-葡萄糖苷酶抑制实验,分别评价了饼干的抗氧化和抗糖尿病能力。结果表明,以高粱粉代替小麦和在饼干原料中添加山竹皮提取物可提高饼干的抗氧化和抗糖尿病活性。从高粱饼干中检测到的化学成分包括山酮类、酚类、类黄酮和生物碱。山竹皮提取物强化高粱饼干很好地接受了小组成员基于感官和享乐的测试。因此,高粱粉和山竹皮可以作为制作健康饼干的绝佳原料。
{"title":"Sorghum cookies fortified with Garcinia mangostana peel extract: Formulation, characterization, and evaluation of antioxidant and antidiabetic activity","authors":"Anastasia Wheni Indrianingsih ,&nbsp;Yuniar Khasanah ,&nbsp;Cici Darsih ,&nbsp;Heru Pitria Hastuti ,&nbsp;Ade Erma Suryani ,&nbsp;Margi Hastuti ,&nbsp;Dwi Ni'maturrohmah ,&nbsp;Umi Laila ,&nbsp;Eka Noviana ,&nbsp;Eka Rahayu ,&nbsp;Tri Wiyono","doi":"10.1016/j.bcdf.2025.100467","DOIUrl":"10.1016/j.bcdf.2025.100467","url":null,"abstract":"<div><div>Increased consumption of processed food and sugary beverages is a major contributor to chronic diseases such as diabetes mellitus and cardiovascular diseases. This research aimed to develop sorghum-based cookie fortified with mangosteen (<em>Garcinia mangostana</em>) peel extract, as a healthy, yet convenient food alternative. Both sorghum and mangosteen are rich in phenolic compounds that have been shown to possess several biological activities including antioxidant and antidiabetic activities. The cookies were evaluated physically and chemically by performing sensory, color, texture, proximate, and chemical content analysis, determination of total phenolic content, and further characterizations using scanning electron microscopy (SEM), X-ray diffraction (XRD), X-ray fluorescence spectroscopy (XRF), and Fourier transform infrared spectroscopy (FTIR). The antioxidant and antidiabetic potencies of the cookies were assessed using radical scavenging and α-glucosidase inhibitory assays, respectively. The results showed that substituting wheat with sorghum flour and adding mangosteen peel extract to the cookie ingredients increased its antioxidant and antidiabetic activity. Several chemical compounds detected from the sorghum cookies included xanthones, phenolics, flavonoids, and alkaloids. The mangosteen peel extract-fortified sorghum cookies were well accepted by the panelists based on sensory and hedonic testing. Hence, sorghum flour and mangosteen peel can be used as excellent ingredients for making healthy cookies.</div></div>","PeriodicalId":38299,"journal":{"name":"Bioactive Carbohydrates and Dietary Fibre","volume":"33 ","pages":"Article 100467"},"PeriodicalIF":0.0,"publicationDate":"2025-01-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143147345","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Guar galactomannan ameliorates radiation-induced intestinal injury in mice 瓜尔半乳甘露聚糖改善小鼠辐射诱导的肠道损伤
Pub Date : 2024-12-30 DOI: 10.1016/j.bcdf.2024.100466
Darshini Shivamogga Mohan , Murali Badanthadka , Anushri Umesh , Bharath Basavapattana Rudresh , Manjunatha Bukkambudhi Krishnaswamy , Rashmi Kanugodu Vasappa , Sahayog Narayan Jamdar , Vidya Shimoga Muddappa
Guar galactomannan (GGM), a widely used hydrocolloid in the food industry, was evaluated for its biological activity with respect to radioprotection. In an in vitro lymphocyte proliferation study, GGM showed significant proliferation of blood lymphocytes at a concentration of 50 μg/ml. The survival study of 30 days showed that GGM pre-treatment improves the survival in irradiated (7.5 Gy) mice to 70% which otherwise is just 30% in the control irradiated group. On day 3, the WBC count was maintained in the GGM pretreated IR mice group compared to the irradiated control group. Serum enzyme levels in the liver of the GGM pretreated irradiated group were reduced, spleen and thymus weight indices and antioxidant enzymes were increased in the intestine of the GGM pretreated irradiated group compared to the irradiated group. Histopathological studies of ileum and jejunum on day 3 revealed that there was less damage in the crypt and height of villi in the GGM pretreated IR mice group compared to the irradiated control group. In the differential gene expression of jejunum, a total of 43 genes showed differential expressions, suggesting their involvement in the biological response to the effect of galactomannan treatment on irradiated mice. Differential expression of genes shows that upregulation of the genes is related to limiting the damage to the intestine and stem cell genes involved in cell differentiation and function. Moreover, genes associated with apoptosis and inflammation were shown to be downregulated in the GGM-pretreated IR group. These results suggest that GGM improves mice from radiation-induced injury by increasing WBC count, reducing acute liver damage, maintaining spleen and thymus weight, increasing the levels of antioxidant enzymes and altering gene expression.
瓜尔半乳甘露聚糖(GGM)是一种广泛应用于食品工业的水胶体,对其放射性防护的生物活性进行了评价。在体外淋巴细胞增殖研究中,GGM浓度为50 μg/ml时,血液淋巴细胞明显增殖。30天的生存研究表明,GGM预处理使7.5 Gy辐照小鼠的存活率提高到70%,而对照组的存活率仅为30%。第3天,与辐照对照组相比,经GGM预处理的IR小鼠组WBC计数维持正常。与辐照组相比,GGM预处理组肝脏血清酶水平降低,脾脏和胸腺重量指数及肠道抗氧化酶水平升高。第3天对回肠和空肠的组织病理学研究显示,与辐照对照组相比,GGM预处理组小鼠的隐窝和绒毛高度损伤较小。在空肠的差异基因表达中,共有43个基因出现差异表达,提示它们参与了半乳甘露聚糖处理对辐照小鼠的生物学反应。基因的差异表达表明,基因的上调与限制肠道和干细胞基因的损伤有关,这些基因参与细胞分化和功能。此外,在ggm预处理的IR组中,与凋亡和炎症相关的基因被显示下调。这些结果表明,GGM通过增加WBC计数、减轻急性肝损伤、维持脾脏和胸腺重量、增加抗氧化酶水平和改变基因表达来改善小鼠辐射损伤。
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引用次数: 0
Potential of curdlan use on the improving textural, cooking and sensory quality, and predicted glycemic index of high-fiber pasta added with watermelon rind 加入西瓜皮的高纤维面食的质构、烹饪品质和感官品质的改善潜力及预测血糖指数
Pub Date : 2024-12-21 DOI: 10.1016/j.bcdf.2024.100464
Dien Quang Long , Thi Thu Tra Tran , Nu Minh Nguyet Ton , Van Viet Man Le
Addition of watermelon rind powder into pasta formulation enhances dietary fiber and antioxidant contents of the fortified pasta but reduces its textural, cooking and sensory quality. In the literature, curdlan has been extensively studied to improve the quality of noodles but its use in the making of durum wheat semolina pasta and high-fiber pasta has not been considered. This study sought to examine the impacts of curdlan addition on the rheological characteristics of dough as well as on the textural and cooking quality, predicted glycemic index, in-vitro protein digestibility and sensory acceptance of high-fiber pasta. In this investigation, different dosages of curdlan were used in the making of high-fiber pasta fortified with watermelon rind and the resulting pasta quality was assessed. When the dosage of curdlan rose from 0 to 2.5% of the blend weight, the breaking strength and breaking distance of raw high-fiber pasta increased significantly. The cohesiveness, hardness, chewiness, elongation rate, tensile strength, cooking quality, and overall acceptability of the supplemented pasta were improved, while the adhesiveness decreased by 76% when comparing pasta samples added with 0% and 2.5% curdlan. The incorporation of curdlan did not have a considerable effect on color of the uncooked pasta samples. Nevertheless, the in-vitro protein digestibility was reduced with increasing curdlan dosage. Importantly, the predicted glycemic index of high-fiber pasta added with 2.5% curdlan was reduced by 1.5 times compared to the pasta sample without curdlan addition, highlighting the potential of curdlan as a food hydrocolloid for enhancing human health. Generally, curdlan was considered a prospective hydrocolloid to improve textural, cooking and sensory quality as well as health effect of the high-fiber pasta fortified with watermelon rind powder.
在面食配方中加入西瓜皮粉可提高强化面食的膳食纤维和抗氧化剂含量,但会降低其质地、烹饪和感官质量。在文献中,凝乳已被广泛研究,以提高面条的质量,但它在制作硬粒小麦粗面粉面食和高纤维面食中的应用尚未被考虑。本研究旨在研究凝乳蛋白添加对面团流变特性的影响,以及对高纤维面食的质地和烹饪质量、预测血糖指数、体外蛋白质消化率和感官接受度的影响。本研究采用不同剂量的凝乳素制作西瓜皮高纤维强化面食,并对其质量进行了评价。当凝乳素用量从掺量的0%增加到掺量的2.5%时,高纤维生面食的断裂强度和断裂距离显著增加。添加0%凝乳蛋白和2.5%凝乳蛋白后,面食的黏结性、硬度、咀嚼性、伸长率、抗拉强度、烹饪质量和整体接受度均有提高,而黏结性比添加0%和2.5%凝乳蛋白的面食降低了76%。凝乳蛋白的加入对未煮熟的意大利面样品的颜色没有很大的影响。然而,随着凝乳素剂量的增加,体外蛋白质消化率降低。重要的是,与未添加凝乳蛋白的面食样品相比,添加2.5%凝乳蛋白的高纤维面食的预测血糖指数降低了1.5倍,这凸显了凝乳蛋白作为一种食品水胶体对人体健康的促进作用。一般认为,凝乳是一种很有前景的水胶体,可以改善西瓜皮粉强化高纤维面食的质地、烹饪和感官品质以及保健效果。
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引用次数: 0
Synbiotics: Effects of prebiotics on the growth and viability of probiotics in food matrices 益生元对食物基质中益生菌生长和活力的影响
Pub Date : 2024-12-01 DOI: 10.1016/j.bcdf.2024.100462
Priyanka Parhi , Shao Quan Liu , Wee Sim Choo
Synbiotics are a combination of prebiotics and probiotics that have a unique effect on their respective counterparts and the host. Probiotics, which are beneficial live microorganisms, have been extensively studied and commercially explored in multiple products worldwide. Numerous scientific studies have established their positive effects on human and animal health. Incorporating prebiotics into probiotic food products has shown different effects on the growth and viability of probiotics. Prebiotics have shown growth-promoting, partial inhibition, dose-dependent, or no effect on the growth and viability of probiotics in different food products. This review discusses synbiotics, probiotics, prebiotics, and specifically the effect of prebiotics on the growth and viability of probiotics in different food matrices. The outcomes depend on multiple factors, including the type and concentration of prebiotics, probiotic strains, and food matrix. Optimization of these parameters can lead to the development of effective synbiotic products that offer numerous human health benefits.
合成益生菌是益生元和益生菌的组合,它们对各自的对应物和宿主具有独特的作用。益生菌是一种有益的活微生物,在世界范围内的多种产品中得到了广泛的研究和商业开发。许多科学研究已经证实了它们对人类和动物健康的积极影响。在益生菌食品中加入益生元对益生菌的生长和活力有不同的影响。益生元对不同食品中益生菌的生长和生存能力有促进、部分抑制、剂量依赖性或无影响的作用。本文综述了合成菌、益生菌、益生元,特别是益生元在不同食物基质中对益生菌生长和活力的影响。结果取决于多种因素,包括益生元的类型和浓度、益生菌菌株和食物基质。这些参数的优化可以导致开发有效的合成产品,提供许多人类健康益处。
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引用次数: 0
Removal notice to “Development of synbiotic juice by spray drying of Lactobacillus paracaesii sub sp. tolerans N2-45 along with galacto-oligossacharides using different carrier materials” [Bioactive Carbohydrates and Dietary Fibre 31 (2024) 100413] 《利用不同载体材料喷雾干燥副干酪乳杆菌耐药菌N2-45及半乳糖低聚糖制备合成果汁》的移除公告[生物活性碳水化合物及膳食纤维31 (2024)100413]
Pub Date : 2024-12-01 DOI: 10.1016/j.bcdf.2024.100451
N.V. Kanimozhi, M. Sukumar
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引用次数: 0
期刊
Bioactive Carbohydrates and Dietary Fibre
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