Pub Date : 2024-03-28DOI: 10.16250/j.32.1374.2023144
W Wu, J Luo, M Huang, M Cai, Y Cheng
Objective: To investigate the population distribution of intermediate host snails and crabs of Paragonimus along the Jiulongjiang River, Zhangjiang River, and Dongxi River basins in Bopingling Mountain, southern Fujian Province, so as to provide baseline data for researches on parasitic disease prevention and control and enlargement of samples in the parasitic resource bank.
Methods: A total of 23 villages in 8 counties (districts) along the Jiulong River, Zhangjiang River, and Dongxi River basins in Zhangzhou City, Fujian Province were selected as survey sites during the period from November 2020 through March 2023, and snail and freshwater crabs were sampled from 1 to 3 streams and ditches neighboring residential areas in each village. Morphological identification of snails was performed according to the external morphological characteristics of collected snail shells, and the unidentified snail species sampled from the natural foci of paragonimiasis in Yunxiao County were subjected to se-quence analysis of the mitochondrial cytochrome oxidase 1 (CO1) gene. The crab species was identified by observing the morphological characteristics of the terminal segment of the first pleopod of male crabs, and Paragonimus cercariae and metacercariae were detected in collected snails.
Results: The shells of the unidentified snails sampled from the natural foci of paragonimiasis in Yunxiao County were approximately 50 mm in height and 18 mm in width, thick and solid, long tower cone-shaped, and had 8 to 10 whorls. CO1 gene sequence analysis identified the snail species as Sulcospira hainanensis. A total of 6 freshwater snail species belonging to 5 genera within 3 families, identified 23 survey sites, including Semisulcospira libertina, Paludomus zhangchouensis and S. hainanensis that belonged to the Family Pleurceridae, Tricula fujianensis and T. huaanensis that belonged to the subfamily Triculinae, Family Pomatiopsidae, and Melanoides tuberculata (Family Thiaridae), and 11 species of freshwater crabs belonging to 5 genera within 2 families were identified, including Sinopotamon genus of S. jianglense, S. pinheense, and S. zhangzhouense, Huananpotamon genus of H. planopodum and H. zhangzhouense, Nanhaipotamon genus of N. huaanense and N. longhaiense, and Minpotamon genus of M. nasicum and M. auritum that belonged to the Family Potamidae, and Somanniathelphusa genus of S. huaanensis and S. zhangpuensis (Family Parathelphusidae). In addition, the prevalence of P. westermani cercariae infections was 0.08% (2/2 317) in P. zhangchouensis from Danyan Village in Changtai District and 0.09% (1/1 039) in S. hainanensis from Jinkeng Village in Yunxiao County, and the prevalence of P. westermani<
目的方法：选择福建省漳州市九龙江、漳江、东溪流域的8个县（区）的23个村为调查点，调查九龙江、漳江、东溪流域寄生虫中间宿主螺蟹的种群分布情况，为寄生虫病防治研究和扩大寄生虫资源库样本提供基础数据：方法：选取福建省漳州市九龙江、漳江、东溪河流域沿岸8个县（区）共23个村作为调查点，于2020年11月至2023年3月期间，在每个村的居民点附近的1至3条溪沟中采集螺类和淡水蟹样本。根据采集螺壳的外部形态特征对螺类进行形态学鉴定，并对云霄县自然疫源地采集的未鉴定螺类进行线粒体细胞色素氧化酶1（CO1）基因序列分析。通过观察雄蟹第一胸足末节的形态特征来确定蟹的种类，并在采集的螺蛳中检测到副鞘蚴和元鞘蚴：结果：在云霄县副银环虫自然疫源地采集到的不明螺类的螺壳高约 50 毫米，宽约 18 毫米，厚而坚实，呈长塔锥形，有 8 至 10 个螺轮。通过 CO1 基因序列分析，确定该螺种为海南螺（Sulcospira hainanensis）。23 个调查地点共发现 3 科 5 属 6 种淡水螺类，包括隶属于褶螺科的半螺、张潮螺和海南螺，以及隶属于褶螺科的福建褶螺和华南褶螺。此外，还鉴定了 2 科 5 属 11 种淡水蟹，其中包括江陵蟹属 Sinopotamon、漳州蟹属 S. P. P.jianglense、S. pinheense 和 S. zhangzhouense 的 Sinopotamon 属，H. planopodum 和 H. zhangzhouense 的 Huananpotamon 属，N. huaanense 和 N. longhaiense 的 Nanhaipotamon 属，以及 M. nasicum 和 M. auritum 的 Minpotamon 属。(Potamidae)，以及 S. huaanensis 和 S. zhangpuensis 的 Somanniathelphusa 属（Parathelphusidae）。此外，长泰县大堰村的漳浦鼠和云霄县金坑村的海南鼠分别感染了 0.08% （2/2 317）和 0.09% （1/1 039）的 P. westermani 氏蛔虫，而云霄县金坑村的漳浦鼠和云霄县金坑村的海南鼠分别感染了 0.09% （1/1 039）和 0.08% （2/2 317）的 P. westermani 元蛔虫。长泰县大堰村的江龙虫和云霄县金坑村的张洲虫的感染率分别为 25.81%（8/31）和 26.31%（5/19）：结论：闽南平顶山九龙江、漳江、东溪流域的中间宿主螺蟹种群存在多样性，首次确认漳州螺和海南螺为西施金线虫的第一中间宿主；闽南平顶山九龙江、漳江、东溪流域的中间宿主螺蟹种群存在多样性，首次确认漳州螺和海南螺为西施金线虫的第一中间宿主；漳州螺和海南螺为西施金线虫的第一中间宿主。
{"title":"[Investigation of the population diversity of intermediate host snails and crabs of <i>Paragonimus</i> along Jiulong River, Zhangjiang River and Dongxi River basins in southern Fujian Province].","authors":"W Wu, J Luo, M Huang, M Cai, Y Cheng","doi":"10.16250/j.32.1374.2023144","DOIUrl":"https://doi.org/10.16250/j.32.1374.2023144","url":null,"abstract":"<p><strong>Objective: </strong>To investigate the population distribution of intermediate host snails and crabs of <i>Paragonimus</i> along the Jiulongjiang River, Zhangjiang River, and Dongxi River basins in Bopingling Mountain, southern Fujian Province, so as to provide baseline data for researches on parasitic disease prevention and control and enlargement of samples in the parasitic resource bank.</p><p><strong>Methods: </strong>A total of 23 villages in 8 counties (districts) along the Jiulong River, Zhangjiang River, and Dongxi River basins in Zhangzhou City, Fujian Province were selected as survey sites during the period from November 2020 through March 2023, and snail and freshwater crabs were sampled from 1 to 3 streams and ditches neighboring residential areas in each village. Morphological identification of snails was performed according to the external morphological characteristics of collected snail shells, and the unidentified snail species sampled from the natural foci of paragonimiasis in Yunxiao County were subjected to se-quence analysis of the mitochondrial cytochrome oxidase 1 (<i>CO1</i>) gene. The crab species was identified by observing the morphological characteristics of the terminal segment of the first pleopod of male crabs, and <i>Paragonimus</i> cercariae and metacercariae were detected in collected snails.</p><p><strong>Results: </strong>The shells of the unidentified snails sampled from the natural foci of paragonimiasis in Yunxiao County were approximately 50 mm in height and 18 mm in width, thick and solid, long tower cone-shaped, and had 8 to 10 whorls. <i>CO1</i> gene sequence analysis identified the snail species as <i>Sulcospira hainanensis</i>. A total of 6 freshwater snail species belonging to 5 genera within 3 families, identified 23 survey sites, including <i>Semisulcospira libertina</i>, <i>Paludomus zhangchouensis</i> and <i>S. hainanensis</i> that belonged to the Family Pleurceridae, <i>Tricula fujianensis</i> and <i>T. huaanensis</i> that belonged to the subfamily Triculinae, Family Pomatiopsidae, and <i>Melanoides tuberculata</i> (Family Thiaridae), and 11 species of freshwater crabs belonging to 5 genera within 2 families were identified, including <i>Sinopotamon</i> genus of <i>S. jianglense</i>, <i>S. pinheense</i>, and <i>S. zhangzhouense</i>, <i>Huananpotamon</i> genus of <i>H. planopodum</i> and <i>H. zhangzhouense</i>, <i>Nanhaipotamon</i> genus of <i>N. huaanense</i> and <i>N. longhaiense</i>, and <i>Minpotamon</i> genus of <i>M. nasicum</i> and <i>M. auritum</i> that belonged to the Family Potamidae, and <i>Somanniathelphusa</i> genus of <i>S. huaanensis</i> and <i>S. zhangpuensis</i> (Family Parathelphusidae). In addition, the prevalence of <i>P. westermani</i> cercariae infections was 0.08% (2/2 317) in <i>P. zhangchouensis</i> from Danyan Village in Changtai District and 0.09% (1/1 039) in <i>S. hainanensis</i> from Jinkeng Village in Yunxiao County, and the prevalence of <i>P. westermani<","PeriodicalId":38874,"journal":{"name":"Chinese Journal of Schistosomiasis Control","volume":"36 1","pages":"44-51"},"PeriodicalIF":0.0,"publicationDate":"2024-03-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140868341","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-03-27DOI: 10.16250/j.32.1374.2023159
Y Liu, H Liu, K Li, Y Liu
Cryptosporidium is an important intestinal parasite that is mainly transmitted through the fecal-oral route. Human infection may occur following ingestion of water and food contaminated by Cryptosporidium oocysts, and children and immunocompromised individuals are at a high risk of infections. The main symptoms of Cryptosporidium infections include diarrhea, vomiting, malnutrition, and even death. Because of high sensitivity and rapid procedures, molecular tests are helpful for the diagnosis of cryptosporidiosis and may reduce the public health risk of cryptosporidiosis. This review summarizes the advances in the latest prevalence and molecular detection of human Cryptosporidium infections during recent years.
{"title":"[Prevalence and molecular detection of human <i>Cryptosporidium</i> infections: a review].","authors":"Y Liu, H Liu, K Li, Y Liu","doi":"10.16250/j.32.1374.2023159","DOIUrl":"10.16250/j.32.1374.2023159","url":null,"abstract":"<p><p><i>Cryptosporidium</i> is an important intestinal parasite that is mainly transmitted through the fecal-oral route. Human infection may occur following ingestion of water and food contaminated by <i>Cryptosporidium</i> oocysts, and children and immunocompromised individuals are at a high risk of infections. The main symptoms of <i>Cryptosporidium</i> infections include diarrhea, vomiting, malnutrition, and even death. Because of high sensitivity and rapid procedures, molecular tests are helpful for the diagnosis of cryptosporidiosis and may reduce the public health risk of cryptosporidiosis. This review summarizes the advances in the latest prevalence and molecular detection of human <i>Cryptosporidium</i> infections during recent years.</p>","PeriodicalId":38874,"journal":{"name":"Chinese Journal of Schistosomiasis Control","volume":"36 1","pages":"105-110"},"PeriodicalIF":0.0,"publicationDate":"2024-03-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140872635","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-03-27DOI: 10.16250/j.32.1374.2023135
Y Chen, D Li, L Wang, C Bu, Y Li, Y Wang, Y Xu, G Yan, B Zhang
Objective: To investigate the prevalence of Anisakis infections in coastal marine fishes and awareness of anisakiasis control knowledge among local residents in Yantai City, Shandong Province in 2021, so as to provide insights into formulation of anisakiasis control interventions.
Methods: Marine fishes were purchased from Shunxin Port, Yantai City, Shandong Province in November 2021, and the presence of Anisakis was detected in different species of fishes and different fish sites. The correlations between body length and weight of marine fish and intensity of Anisakis infections were examined using Spearman's rank correlation analysis, and the dietary habits and anisakiasis control knowledge were investigated using questionnaire surveys among local residents.
Results: A total of 201 marine fishes belonging to 20 species were dissected, and Anisakis was detected in 77 marine fishes (38.31%) belonging to 11 species (55.00%), with a mean infection intensity of 45.04 parasites per fish (3 468/77). Spearman's rank correlation analysis revealed that the body length (rs = 0.74, P < 0.05) and weight (rs = 0.79, P < 0.01) of the monkfish correlated positively with the intensity of Anisakis infections, and the body length (rs = 0.68, P < 0.05) of the flatfish correlated positively with the intensity of Anisakis infections, while no correlations were examined between the body length or weight of other marine fishes and the intensity of Anisakis infections. Of all respondents, 53.38% men and 56.67% women did not know anisakiasis control knowledge at all, and there was a significant difference in the proportion of respondents using separate chopping boards for raw and cooked food from different villages (χ2 = 17.89, P < 0.01), while there was an age-specific proportion of respondents with habitats of eating raw or semi-raw seafood (χ2 = 28.27, P < 0.01).
Conclusions: The prevalence and intensity of Anisakis infections were high in coastal marine fishes in Yantai City in 2021, and the awareness of anisakiasis control knowledge was low among local residents. Intensified health education pertaining to anisakiasis control knowledge is recommended to reduce the risk of Anisakis infections.
{"title":"[Investigation of <i>Anisakis</i> infections in coastal marine fishes and awareness of anisakiasis control knowledge among residents in Yantai City in 2021].","authors":"Y Chen, D Li, L Wang, C Bu, Y Li, Y Wang, Y Xu, G Yan, B Zhang","doi":"10.16250/j.32.1374.2023135","DOIUrl":"https://doi.org/10.16250/j.32.1374.2023135","url":null,"abstract":"<p><strong>Objective: </strong>To investigate the prevalence of <i>Anisakis</i> infections in coastal marine fishes and awareness of anisakiasis control knowledge among local residents in Yantai City, Shandong Province in 2021, so as to provide insights into formulation of anisakiasis control interventions.</p><p><strong>Methods: </strong>Marine fishes were purchased from Shunxin Port, Yantai City, Shandong Province in November 2021, and the presence of <i>Anisakis</i> was detected in different species of fishes and different fish sites. The correlations between body length and weight of marine fish and intensity of <i>Anisakis</i> infections were examined using Spearman's rank correlation analysis, and the dietary habits and anisakiasis control knowledge were investigated using questionnaire surveys among local residents.</p><p><strong>Results: </strong>A total of 201 marine fishes belonging to 20 species were dissected, and <i>Anisakis</i> was detected in 77 marine fishes (38.31%) belonging to 11 species (55.00%), with a mean infection intensity of 45.04 parasites per fish (3 468/77). Spearman's rank correlation analysis revealed that the body length (<i>r</i><sub>s</sub> = 0.74, <i>P</i> < 0.05) and weight (<i>r</i><sub>s</sub> = 0.79, <i>P</i> < 0.01) of the monkfish correlated positively with the intensity of <i>Anisakis</i> infections, and the body length (<i>r</i><sub>s</sub> = 0.68, <i>P</i> < 0.05) of the flatfish correlated positively with the intensity of <i>Anisakis</i> infections, while no correlations were examined between the body length or weight of other marine fishes and the intensity of <i>Anisakis</i> infections. Of all respondents, 53.38% men and 56.67% women did not know anisakiasis control knowledge at all, and there was a significant difference in the proportion of respondents using separate chopping boards for raw and cooked food from different villages (χ<sup>2</sup> = 17.89, <i>P</i> < 0.01), while there was an age-specific proportion of respondents with habitats of eating raw or semi-raw seafood (χ<sup>2</sup> = 28.27, <i>P</i> < 0.01).</p><p><strong>Conclusions: </strong>The prevalence and intensity of <i>Anisakis</i> infections were high in coastal marine fishes in Yantai City in 2021, and the awareness of anisakiasis control knowledge was low among local residents. Intensified health education pertaining to anisakiasis control knowledge is recommended to reduce the risk of <i>Anisakis</i> infections.</p>","PeriodicalId":38874,"journal":{"name":"Chinese Journal of Schistosomiasis Control","volume":"36 1","pages":"74-78"},"PeriodicalIF":0.0,"publicationDate":"2024-03-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140868340","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-03-25DOI: 10.16250/j.32.1374.2023212
W Li, X Wang, L Ma, X Dou, R Zhao, Y Wang
This case report summarizes the experience from diagnosis and treatment of a patient with repeated high fever, hepatosplenomegaly and pancytopenia. Following exclusion of bacterial, viral, fungal infections and hematological diseases, metagenomic next-generation sequencing of the patient's peripheral blood revealed Leishmania infantum infection, and rK39 rapid diagnostic test showed positive for anti-Leishmania antibody, while microscopic examination of bone marrow smears identified Leishmania amastigotes. Therefore, the case was definitively diagnosed as visceral leishmaniasis, and given anti-infective treatment with sodium antimony gluconate and hormone, hepatoprotection, elevation of white blood cell counts and personalized nursing. Then, the case was cured and discharged from hospital. Metagenomic next-generation sequencing is of great value in etiological detection of fever patients with unknown causes, which deserves widespread clinical applications.
{"title":"[Auxiliary diagnosis of visceral leishmaniasis with metagenomic next-generation sequencing: a case report].","authors":"W Li, X Wang, L Ma, X Dou, R Zhao, Y Wang","doi":"10.16250/j.32.1374.2023212","DOIUrl":"10.16250/j.32.1374.2023212","url":null,"abstract":"<p><p>This case report summarizes the experience from diagnosis and treatment of a patient with repeated high fever, hepatosplenomegaly and pancytopenia. Following exclusion of bacterial, viral, fungal infections and hematological diseases, metagenomic next-generation sequencing of the patient's peripheral blood revealed <i>Leishmania infantum</i> infection, and rK39 rapid diagnostic test showed positive for anti-<i>Leishmania</i> antibody, while microscopic examination of bone marrow smears identified <i>Leishmania</i> amastigotes. Therefore, the case was definitively diagnosed as visceral leishmaniasis, and given anti-infective treatment with sodium antimony gluconate and hormone, hepatoprotection, elevation of white blood cell counts and personalized nursing. Then, the case was cured and discharged from hospital. Metagenomic next-generation sequencing is of great value in etiological detection of fever patients with unknown causes, which deserves widespread clinical applications.</p>","PeriodicalId":38874,"journal":{"name":"Chinese Journal of Schistosomiasis Control","volume":"36 2","pages":"215-218"},"PeriodicalIF":0.0,"publicationDate":"2024-03-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141301726","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-03-25DOI: 10.16250/j.32.1374.2023167
D Li, Y Li, Y Yu, X Ouyang, X Xiong, S Jin, J Jiao
Objective: To investigate the prevalence of tick-borne rickettsial infections in selected areas of Liupanshui City, Guizhou Province, 2023, so as to provide insights into the management of tick-borne rickettsioses in the city.
Methods: Ticks were captured from the body surface of bovines and sheep in Gaoxing Village, Dashan Township, Liupanshui City, Guizhou Province during the period between April and June, 2023, and tick species were identified using morphological and molecular biological techniques. In addition, tick-borne Rickettsia was identified using a nested PCR assay, including spotted fever group rickettsiae (SFGR), Coxiella spp., Anaplasma spp., Ehrlichia spp., and Orientia spp., and positive amplified fragments were sequenced and aligned with known sequences accessed in the GenBank database.
Results: A total of 200 ticks were collected and all tick species were identified as Rhipicephalus microplus. Nestle PCR assay combined with sequencing identified ticks carrying Candidatus Rickettsia jingxinensis (40.50%), Coxiella burnetii (1.50%), and Coxiella-like endosymbionts (27.00%), and Anaplasma spp., Ehrlichia spp. or Orientsia spp. was not detected.
Conclusions: R. microplus carried Candidatus R. jingxinensis, C. burnetii, and Coxiella-like endosymbionts in selected areas of Liupanshui City, Guizhou Province. Intensified monitoring of tickborne rickettsial infections is needed in livestock and humans to reduce the damages caused by rickettsioses.
{"title":"[Investigation of tick - borne <i>Rickettsia</i> in selected areas of Liupanshui City, Guizhou Province in 2023].","authors":"D Li, Y Li, Y Yu, X Ouyang, X Xiong, S Jin, J Jiao","doi":"10.16250/j.32.1374.2023167","DOIUrl":"https://doi.org/10.16250/j.32.1374.2023167","url":null,"abstract":"<p><strong>Objective: </strong>To investigate the prevalence of tick-borne rickettsial infections in selected areas of Liupanshui City, Guizhou Province, 2023, so as to provide insights into the management of tick-borne rickettsioses in the city.</p><p><strong>Methods: </strong>Ticks were captured from the body surface of bovines and sheep in Gaoxing Village, Dashan Township, Liupanshui City, Guizhou Province during the period between April and June, 2023, and tick species were identified using morphological and molecular biological techniques. In addition, tick-borne <i>Rickettsia</i> was identified using a nested PCR assay, including spotted fever group rickettsiae (SFGR), <i>Coxiella</i> spp., <i>Anaplasma</i> spp., <i>Ehrlichia</i> spp., and <i>Orientia</i> spp., and positive amplified fragments were sequenced and aligned with known sequences accessed in the GenBank database.</p><p><strong>Results: </strong>A total of 200 ticks were collected and all tick species were identified as <i>Rhipicephalus microplus</i>. Nestle PCR assay combined with sequencing identified ticks carrying <i>Candidatus Rickettsia jingxinensis</i> (40.50%), <i>Coxiella burnetii</i> (1.50%), and <i>Coxiella</i>-like endosymbionts (27.00%), and <i>Anaplasma</i> spp., <i>Ehrlichia</i> spp. or <i>Orientsia</i> spp. was not detected.</p><p><strong>Conclusions: </strong><i>R. microplus</i> carried <i>Candidatus R. jingxinensis</i>, <i>C. burnetii</i>, and <i>Coxiella</i>-like endosymbionts in selected areas of Liupanshui City, Guizhou Province. Intensified monitoring of tickborne rickettsial infections is needed in livestock and humans to reduce the damages caused by rickettsioses.</p>","PeriodicalId":38874,"journal":{"name":"Chinese Journal of Schistosomiasis Control","volume":"36 2","pages":"154-158"},"PeriodicalIF":0.0,"publicationDate":"2024-03-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141301794","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-03-25DOI: 10.16250/j.32.1374.2023157
J Lou, X He
Parasitic diseases caused by protozoan and helminth infections are still widespread across the world, notably in tropical and subtropical areas, which threaten the children and adult health. Long-term use of anti-parasitic drugs may result in reduced drug susceptibility and even drug resistance. Antimicrobial peptides have been demonstrated to inhibit parasite growth and development, which has potential antiparasitic values. LL-37, the only human antimicrobial peptide in the cathelicidin family, has been widely investigated. This paper reviews the progress of researches on the antiparasitic activity of LL-37, and discusses the prospects of LL-37 in the research of parasites.
{"title":"[Progress of researches on the antiparasitic activity of antimicrobial peptide LL-37].","authors":"J Lou, X He","doi":"10.16250/j.32.1374.2023157","DOIUrl":"https://doi.org/10.16250/j.32.1374.2023157","url":null,"abstract":"<p><p>Parasitic diseases caused by protozoan and helminth infections are still widespread across the world, notably in tropical and subtropical areas, which threaten the children and adult health. Long-term use of anti-parasitic drugs may result in reduced drug susceptibility and even drug resistance. Antimicrobial peptides have been demonstrated to inhibit parasite growth and development, which has potential antiparasitic values. LL-37, the only human antimicrobial peptide in the cathelicidin family, has been widely investigated. This paper reviews the progress of researches on the antiparasitic activity of LL-37, and discusses the prospects of LL-37 in the research of parasites.</p>","PeriodicalId":38874,"journal":{"name":"Chinese Journal of Schistosomiasis Control","volume":"36 1","pages":"98-104"},"PeriodicalIF":0.0,"publicationDate":"2024-03-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140872677","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-03-25DOI: 10.16250/j.32.1374.2023178
Y Wang, J Xu, C Xia
Schistosomiasis is a neglected zoonotic parasitic disease. Currently, praziquantel is the drug of choice for the treatment of schistosomiasis, and is the only effective chemical for treatment of schistosomiasis japonica. Since its introduction in the 1970s, praziquantel has been used for large-scale chemotherapy of schistosomiasis for over 40 years. However, there have been reports pertaining to the resistance to praziquantel in schistosomes. Therefore, development of novel antischistosomal agents as alternatives of praziquantel, is of great need. Histone deacetylases and histone acetyltransferases have been recently reported to play critical roles in the growth, development and reproduction of schistosomes, and are considered as potential drug targets for the treatment of schistosomiasis. This review summarizes the latest advances of histone deacetylase and histone acetyltransferase inhibitors in the research on antischistosomal drugs, so as to provide insights into research and development of novelantischistosomal agents.
{"title":"[Histone deacetylase and histone acetyltransferase inhibitors as antischistosomal agents: a review].","authors":"Y Wang, J Xu, C Xia","doi":"10.16250/j.32.1374.2023178","DOIUrl":"10.16250/j.32.1374.2023178","url":null,"abstract":"<p><p>Schistosomiasis is a neglected zoonotic parasitic disease. Currently, praziquantel is the drug of choice for the treatment of schistosomiasis, and is the only effective chemical for treatment of schistosomiasis japonica. Since its introduction in the 1970s, praziquantel has been used for large-scale chemotherapy of schistosomiasis for over 40 years. However, there have been reports pertaining to the resistance to praziquantel in schistosomes. Therefore, development of novel antischistosomal agents as alternatives of praziquantel, is of great need. Histone deacetylases and histone acetyltransferases have been recently reported to play critical roles in the growth, development and reproduction of schistosomes, and are considered as potential drug targets for the treatment of schistosomiasis. This review summarizes the latest advances of histone deacetylase and histone acetyltransferase inhibitors in the research on antischistosomal drugs, so as to provide insights into research and development of novelantischistosomal agents.</p>","PeriodicalId":38874,"journal":{"name":"Chinese Journal of Schistosomiasis Control","volume":"36 2","pages":"207-214"},"PeriodicalIF":0.0,"publicationDate":"2024-03-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141301793","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-03-21DOI: 10.16250/j.32.1374.2023169
Y Zhang, J Wu, S Adili, S Wang, H Zhang, G Shi, J Zhao
Objective: To analyze the echinococcosis surveillance results in Bayingolin Mongol Autonomous Prefecture, Xinjiang Uygur Autonomous Region from 2017 to 2022, so as to provide insights into formulation of echinococcosis control measures in the prefecture.
Methods: Villagers were randomly sampled using a multistage sampling method from class I and II echinococcosis endemic counties in Bayingolin Mongolian Autonomous Prefecture from 2017 to 2022 for detection of human echinococcosis, while all patients undergoing ultrasound examinations in medical institutions in class III endemic counties received active echinococcosis screening. In addition, livestock in centralized slaughterhouses or slaughtering sites were screened for echinococcosis using the palpation and necropsy method, and fresh domestic dog feces samples were collected from randomly selected dog owners in each administrative village for detection of Echinococcus copro-antigen in domestic dogs. The trends in detection of human and livestock echinococcosis, detection of newly diagnosed human echinococcosis cases and detection of Echinococcus coproantigen in domestic dogs were analyzed in Bayingolin Mongol Autonomous Prefecture from 2017 to 2022.
Results: The mean detection rate of human echinococcosis was 0.13% (540/407 803) in Bayingolin Mongol Autonomous Prefecture from 2017 to 2022, which appeared a tendency towards a decline over years (χ2trend = 1 217.21, P < 0.001), and the highest detection of newly diagnosed echinococcosis cases was seen in Hejing County (0.28%, 191/67 865). The detection of livestock echinococcosis appeared a tendency towards a decline over years from 2017 to 2022 (χ2trend = 147.02, P < 0.001), with the highest detection rate seen in Hejing County (3.44%, 86/2 500), and the detection of Echinococcus copro-antigen in domestic dogs appeared a tendency towards a decline over years from 2017 to 2022 (χ2trend = 302.46, P < 0.001), with the highest detection rate in Qiemo County (2.74%, 118/4 313).
Conclusions: The detection of human and livestock echinococcosis and dog feces antigens Echinococcus copro-antigen in domestic dogs all appeared a tendency towards a decline in Bayingolin Mongol Autonomous Prefecture, Xinjiang Uygur Autonomous Region from 2017 to 2022; however, there is still a high echinococcosis transmission risk in local areas. Sustainable integrated echinococcosis control is required in Bayingolin Mongol Autonomous Prefecture.
{"title":"[Surveillance of echinococcosis in Bayingolin Mongol Autonomous Prefecture, Xinjiang Uygur Autonomous Region from 2017 to 2022].","authors":"Y Zhang, J Wu, S Adili, S Wang, H Zhang, G Shi, J Zhao","doi":"10.16250/j.32.1374.2023169","DOIUrl":"https://doi.org/10.16250/j.32.1374.2023169","url":null,"abstract":"<p><strong>Objective: </strong>To analyze the echinococcosis surveillance results in Bayingolin Mongol Autonomous Prefecture, Xinjiang Uygur Autonomous Region from 2017 to 2022, so as to provide insights into formulation of echinococcosis control measures in the prefecture.</p><p><strong>Methods: </strong>Villagers were randomly sampled using a multistage sampling method from class I and II echinococcosis endemic counties in Bayingolin Mongolian Autonomous Prefecture from 2017 to 2022 for detection of human echinococcosis, while all patients undergoing ultrasound examinations in medical institutions in class III endemic counties received active echinococcosis screening. In addition, livestock in centralized slaughterhouses or slaughtering sites were screened for echinococcosis using the palpation and necropsy method, and fresh domestic dog feces samples were collected from randomly selected dog owners in each administrative village for detection of <i>Echinococcus</i> copro-antigen in domestic dogs. The trends in detection of human and livestock echinococcosis, detection of newly diagnosed human echinococcosis cases and detection of <i>Echinococcus</i> coproantigen in domestic dogs were analyzed in Bayingolin Mongol Autonomous Prefecture from 2017 to 2022.</p><p><strong>Results: </strong>The mean detection rate of human echinococcosis was 0.13% (540/407 803) in Bayingolin Mongol Autonomous Prefecture from 2017 to 2022, which appeared a tendency towards a decline over years (χ<sup>2</sup><sub>trend</sub> = 1 217.21, <i>P</i> < 0.001), and the highest detection of newly diagnosed echinococcosis cases was seen in Hejing County (0.28%, 191/67 865). The detection of livestock echinococcosis appeared a tendency towards a decline over years from 2017 to 2022 (χ<sup>2</sup><sub>trend</sub> = 147.02, <i>P</i> < 0.001), with the highest detection rate seen in Hejing County (3.44%, 86/2 500), and the detection of <i>Echinococcus</i> copro-antigen in domestic dogs appeared a tendency towards a decline over years from 2017 to 2022 (χ<sup>2</sup><sub>trend</sub> = 302.46, <i>P</i> < 0.001), with the highest detection rate in Qiemo County (2.74%, 118/4 313).</p><p><strong>Conclusions: </strong>The detection of human and livestock echinococcosis and dog feces antigens <i>Echinococcus</i> copro-antigen in domestic dogs all appeared a tendency towards a decline in Bayingolin Mongol Autonomous Prefecture, Xinjiang Uygur Autonomous Region from 2017 to 2022; however, there is still a high echinococcosis transmission risk in local areas. Sustainable integrated echinococcosis control is required in Bayingolin Mongol Autonomous Prefecture.</p>","PeriodicalId":38874,"journal":{"name":"Chinese Journal of Schistosomiasis Control","volume":"36 1","pages":"79-82"},"PeriodicalIF":0.0,"publicationDate":"2024-03-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140854029","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-03-18DOI: 10.16250/j.32.1374.2023208
J He, S Li, W Deng, C Cao, S Li, J Xu
Objective: To understand the current status of capacity building in schistosomiasis control institutes in schistosomiasis-endemic provinces (municipality, autonomous region) of China.
Methods: The responsibilities and construction requirements of various schistosomiasis control institutions were surveyed by expert discussions, and field interviews and visits during the period between May and June, 2023, and the questionnaire for capacity maintenance and consolidation in schistosomiasis control institutions was designed. An online questionnaire survey was conducted in county-, municipal-, and provincial-level institutions that undertook schistosomiasis control and surveillance activities through the Wenjuanxing program. The distribution of schistosomiasis control institutions, the status of institutions, departments and staff undertaking schistosomiasis control activities and the translation of scientific researches on schistosomiasis control in China were analyzed. The laboratories accredited by China National Accreditation Service for Conformity Assessment (CNAS) were considered to be capable for testing associated with schistosomiasis control, and the testing capability of schistosomiasis control institutions was analyzed.
Results: A total of 486 valid questionnaires were recovered from 486 schistosomiasis control institutions in 12 endemic provinces (municipality, autonomous region) of China, including 12 provincial-level institutions (2.5%), 77 municipal-level institutions (15.8%) and 397 county-level institutions (81.7%). Of all schistosomiasis control institutions, 376 (77.4%) were centers for disease control and prevention or public health centers, 102 (21.0%) were institutions for schistosomiasis, endemic disease and parasitic disease control, and 8 (1.6%) were hospitals, healthcare centers or others. There were 37 713 active employees in the 486 schistosomiasis control institutions, including 5 675 employees related to schistosomiasis control, and the proportions of employees associated with schistosomiasis control among all active employees were 5.9% (231/3 897), 5.5% (566/10 134), and 20.6% (4 878/23 682) in provincial-, municipal-, and county-level institutions, respectively. There were 3 826 full-time employees working in schistosomiasis control activities, with 30.5% (1 166/3 826), 34.6% (1 324) and 34.9% (1 336/3 826) at ages of 40 years and below, 41 to 50 years and over 50 years, and there were 1 571 (41.0%) full-time schistosomiasis control employees with duration of schistosomiasis control activities for over 25 years, and 1 358 (35.5%) employees with junior professional titles and 1 290 with intermediate professional titles (35.5%), while 712 (18.6%) full-time employees working in schistosomiasis control activities had no professional titles. The three core schistosomiasis control activities included snail control (26.3%, 374/1 420), epidemics surveillance and m
{"title":"[Capacity building in schistosomiasis control institutions in China: a cross-sectional study].","authors":"J He, S Li, W Deng, C Cao, S Li, J Xu","doi":"10.16250/j.32.1374.2023208","DOIUrl":"https://doi.org/10.16250/j.32.1374.2023208","url":null,"abstract":"<p><strong>Objective: </strong>To understand the current status of capacity building in schistosomiasis control institutes in schistosomiasis-endemic provinces (municipality, autonomous region) of China.</p><p><strong>Methods: </strong>The responsibilities and construction requirements of various schistosomiasis control institutions were surveyed by expert discussions, and field interviews and visits during the period between May and June, 2023, and the questionnaire for capacity maintenance and consolidation in schistosomiasis control institutions was designed. An online questionnaire survey was conducted in county-, municipal-, and provincial-level institutions that undertook schistosomiasis control and surveillance activities through the Wenjuanxing program. The distribution of schistosomiasis control institutions, the status of institutions, departments and staff undertaking schistosomiasis control activities and the translation of scientific researches on schistosomiasis control in China were analyzed. The laboratories accredited by China National Accreditation Service for Conformity Assessment (CNAS) were considered to be capable for testing associated with schistosomiasis control, and the testing capability of schistosomiasis control institutions was analyzed.</p><p><strong>Results: </strong>A total of 486 valid questionnaires were recovered from 486 schistosomiasis control institutions in 12 endemic provinces (municipality, autonomous region) of China, including 12 provincial-level institutions (2.5%), 77 municipal-level institutions (15.8%) and 397 county-level institutions (81.7%). Of all schistosomiasis control institutions, 376 (77.4%) were centers for disease control and prevention or public health centers, 102 (21.0%) were institutions for schistosomiasis, endemic disease and parasitic disease control, and 8 (1.6%) were hospitals, healthcare centers or others. There were 37 713 active employees in the 486 schistosomiasis control institutions, including 5 675 employees related to schistosomiasis control, and the proportions of employees associated with schistosomiasis control among all active employees were 5.9% (231/3 897), 5.5% (566/10 134), and 20.6% (4 878/23 682) in provincial-, municipal-, and county-level institutions, respectively. There were 3 826 full-time employees working in schistosomiasis control activities, with 30.5% (1 166/3 826), 34.6% (1 324) and 34.9% (1 336/3 826) at ages of 40 years and below, 41 to 50 years and over 50 years, and there were 1 571 (41.0%) full-time schistosomiasis control employees with duration of schistosomiasis control activities for over 25 years, and 1 358 (35.5%) employees with junior professional titles and 1 290 with intermediate professional titles (35.5%), while 712 (18.6%) full-time employees working in schistosomiasis control activities had no professional titles. The three core schistosomiasis control activities included snail control (26.3%, 374/1 420), epidemics surveillance and m","PeriodicalId":38874,"journal":{"name":"Chinese Journal of Schistosomiasis Control","volume":"36 1","pages":"67-73"},"PeriodicalIF":0.0,"publicationDate":"2024-03-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140852815","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-02-26DOI: 10.16250/j.32.1374.2023172
Z Renzeng, H Fan, K Yang, Z Wang, Y Zhang, Y Lu, H Wang
Objective: To investigate the expression of neutrophil extracellular traps (NETs) and phagocytic function in the peripheral blood of patients with hepatic alveolar echinococcosis (HAE), and to examine their correlations with clinical inflamma tory indicators and liver functions.
Methods: A total of 50 patients with HAE admitted to Department of Hepatobiliary and Pancreatic Surgery, The Affiliated Hospital of Qinghai University from August 2022 to June 2023 were enrolled, while 50 age- and gender-matched healthy individuals from the Centre for Healthy Examinations of the hospital during the same period served as controls. The levels of NETs markers neutrophil myeloperoxidase (MPO) and neutrophil elastase (NE) were measured using enzyme-linked immunosorbent assay (ELISA). Peripheral blood neutrophils were isolated using density gradient centrifugation, stimulated in vitro using phorbol 12-myristate 13 acetate (PMA), and the levels of MPO and citrullination histone H3 (CitH3) released by neutrophils were quantified using flow cytometry. The phagocytic functions of neutrophils were examined using flow cytometry. In addition, the correlations of MPO and NE levels with clinical inflammatory indicators and liver biochemical indicators were examined using Spearman correlation analysis among HAE patients.
Results: The peripheral blood plasma MPO［(417.15 ± 76.08) ng/mL vs. (255.70 ± 80.84) ng/mL; t = 10.28, P < 0.05］, NE［(23.16 ± 6.75) ng/mL vs. (11.92 ± 3.17) ng/mL; t = 10.65, P < 0.05］and CitH3 levels［(33.93 ± 18.93) ng/mL vs. (19.52 ± 13.89) ng/mL; t = 4.34, P < 0.05］were all significantly higher among HAE patients than among healthy controls, and a lower phagocytosis rate of neutrophils was detected among HAE patients than among healthy controls［(70.85 ± 7.32)% vs. (94.04 ± 3.90)%; t = 20.18, P < 0.05］, and the ability to produce NETs by neutrophils was higher among HAE patients than among healthy controls following in vitro PMA stimulation. Pearson correlation analysis showed that the phagocytosis rate of neutrophils correlated negatively with platelet-to-lymphocyte ratio (PLR), neutrophil-to-lymphocyte ratio (NLR), interleukin-6 (IL-6) level and C-reactive protein (CRP) level (rs = -0.515 to -0.392, all P values < 0.05), and the MPO and NE levels positively correlated with inflammatory markers NLR, PLR, CRP and IL-6 (rs = 0.333 to 0.445, all P values < 0.05) and clinical liver biochemical indicators aspartic transaminase, alanine aminotransferase, direct bilirubin and total bilirubin among HAE patients (rs = 0.290 to 0.628, all P values < 0.001).
Conclusions: Excessive formation of NETs is found among HAE patients, which affects the phagocytic ability of neutrophils and results in elevated levels of inflammatory indicato
研究目的研究肝泡型棘球蚴病（HAE）患者外周血中中性粒细胞胞外捕获物（NETs）的表达及吞噬功能，并探讨其与临床炎症指标及肝功能的相关性：方法：选取青海大学附属医院肝胆胰外科2022年8月至2023年6月收治的50例HAE患者作为研究对象，同时选取该院体检中心同期收治的50例年龄、性别匹配的健康人作为对照。采用酶联免疫吸附试验（ELISA）测量NETs标志物中性粒细胞髓过氧化物酶（MPO）和中性粒细胞弹性蛋白酶（NE）的水平。使用密度梯度离心法分离外周血中性粒细胞，使用硫醇-12-肉豆蔻酸 13 乙酸酯（PMA）体外刺激中性粒细胞，使用流式细胞术量化中性粒细胞释放的 MPO 和瓜氨酸化组蛋白 H3（CitH3）的水平。使用流式细胞术检测了中性粒细胞的吞噬功能。此外，还利用斯皮尔曼相关分析法检测了HAE患者MPO和NE水平与临床炎症指标和肝脏生化指标的相关性：外周血血浆MPO［(417.15 ± 76.08) ng/mL vs. (255.70 ± 80.84) ng/mL; t = 10.28, P < 0.05］，NE［(23.16 ± 6.75) ng/mL vs. (11.92 ± 3.17) ng/mL; t = 10.28, P < 0.05］。(11.92 ± 3.17) ng/mL; t = 10.65, P < 0.05］和CitH3水平［(33.93 ± 18.93) ng/mL vs. (19.52 ± 13.89) ng/mL; t = 4.34, P < 0.HAE患者中性粒细胞吞噬率低于健康对照组［（70.85 ± 7.32）% vs. （94.04 ± 3.90）%；t = 20.18，P < 0.05］，体外PMA刺激后中性粒细胞产生NET的能力高于健康对照组。皮尔逊相关分析显示，中性粒细胞的吞噬率与血小板-淋巴细胞比值（PLR）、中性粒细胞-淋巴细胞比值（NLR）、白细胞介素-6（IL-6）水平和C反应蛋白（CRP）水平呈负相关（rs = -0.515至-0.392，所有P值均＜0.05），MPO和NE水平与炎症指标NLR、PLR、CRP和IL-6呈正相关（rs = 0.333至0.445，所有P值均<0.05），HAE患者的临床肝脏生化指标天冬氨酸转氨酶、丙氨酸转氨酶、直接胆红素和总胆红素呈正相关（rs = 0.290至0.628，所有P值均<0.001）：结论：HAE患者体内NETs形成过多，影响了中性粒细胞的吞噬能力，导致炎症指标水平升高。NETs标记物可能是有希望用于肝病早期诊断、监测和严重程度评估的新型生物标记物。
{"title":"[Expression of neutrophil extracellular traps and phagocytic functions among patients with hepatic alveolar echinococcosis].","authors":"Z Renzeng, H Fan, K Yang, Z Wang, Y Zhang, Y Lu, H Wang","doi":"10.16250/j.32.1374.2023172","DOIUrl":"https://doi.org/10.16250/j.32.1374.2023172","url":null,"abstract":"<p><strong>Objective: </strong>To investigate the expression of neutrophil extracellular traps (NETs) and phagocytic function in the peripheral blood of patients with hepatic alveolar echinococcosis (HAE), and to examine their correlations with clinical inflamma tory indicators and liver functions.</p><p><strong>Methods: </strong>A total of 50 patients with HAE admitted to Department of Hepatobiliary and Pancreatic Surgery, The Affiliated Hospital of Qinghai University from August 2022 to June 2023 were enrolled, while 50 age- and gender-matched healthy individuals from the Centre for Healthy Examinations of the hospital during the same period served as controls. The levels of NETs markers neutrophil myeloperoxidase (MPO) and neutrophil elastase (NE) were measured using enzyme-linked immunosorbent assay (ELISA). Peripheral blood neutrophils were isolated using density gradient centrifugation, stimulated <i>in vitro</i> using phorbol 12-myristate 13 acetate (PMA), and the levels of MPO and citrullination histone H3 (CitH3) released by neutrophils were quantified using flow cytometry. The phagocytic functions of neutrophils were examined using flow cytometry. In addition, the correlations of MPO and NE levels with clinical inflammatory indicators and liver biochemical indicators were examined using Spearman correlation analysis among HAE patients.</p><p><strong>Results: </strong>The peripheral blood plasma MPO［(417.15 ± 76.08) ng/mL vs. (255.70 ± 80.84) ng/mL; <i>t</i> = 10.28, <i>P</i> < 0.05］, NE［(23.16 ± 6.75) ng/mL vs. (11.92 ± 3.17) ng/mL; <i>t</i> = 10.65, <i>P</i> < 0.05］and CitH3 levels［(33.93 ± 18.93) ng/mL vs. (19.52 ± 13.89) ng/mL; <i>t</i> = 4.34, <i>P</i> < 0.05］were all significantly higher among HAE patients than among healthy controls, and a lower phagocytosis rate of neutrophils was detected among HAE patients than among healthy controls［(70.85 ± 7.32)% vs. (94.04 ± 3.90)%; <i>t</i> = 20.18, <i>P</i> < 0.05］, and the ability to produce NETs by neutrophils was higher among HAE patients than among healthy controls following <i>in vitro</i> PMA stimulation. Pearson correlation analysis showed that the phagocytosis rate of neutrophils correlated negatively with platelet-to-lymphocyte ratio (PLR), neutrophil-to-lymphocyte ratio (NLR), interleukin-6 (IL-6) level and C-reactive protein (CRP) level (<i>r</i><sub>s</sub> = -0.515 to -0.392, all <i>P</i> values < 0.05), and the MPO and NE levels positively correlated with inflammatory markers NLR, PLR, CRP and IL-6 (<i>r</i><sub>s</sub> = 0.333 to 0.445, all <i>P</i> values < 0.05) and clinical liver biochemical indicators aspartic transaminase, alanine aminotransferase, direct bilirubin and total bilirubin among HAE patients (<i>r</i><sub>s</sub> = 0.290 to 0.628, all <i>P</i> values < 0.001).</p><p><strong>Conclusions: </strong>Excessive formation of NETs is found among HAE patients, which affects the phagocytic ability of neutrophils and results in elevated levels of inflammatory indicato","PeriodicalId":38874,"journal":{"name":"Chinese Journal of Schistosomiasis Control","volume":"36 1","pages":"25-33"},"PeriodicalIF":0.0,"publicationDate":"2024-02-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140866762","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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