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Studies on anatomical behaviour of PaLCuV infected papaya (Carica papaya L.) PaLCuV感染番木瓜的解剖行为研究
Q4 Biochemistry, Genetics and Molecular Biology Pub Date : 2018-12-15 DOI: 10.37855/jah.2018.v20i03.38
M. Mishra, M. Mishra, Shantha Selva Kumari, P. Shirke, A. Srivastava, S. Saxena
Papaya leaf curl virus (PaLCuV) of geminiviridae family is a major threat to papaya plants in the world. The major visual characteristics of PaLCuV infected plants are downward and inward rolling and curling of leaves in the form of an inverted cup and thickening of veins. Microscopic observation showed that in the healthy papaya leaf, stomata guard cell size was 19-20 μm. However, it increased significantly in infected plant leaves up to 29-30 μm. This observation suggested that stomatal density and guard cell size were changed due to puckered anatomy of leaf. SEM analysis revealed that subsidiary or accessory cells of guard cells were less turgid and the arrangement of starch grains was disturbed as compared to healthy plant leaves. Light microscopic, scanning electron microscopy (SEM) and energy-dispersive X-ray spectroscopy (EDS) analyses of symptomatic leaves showed the puckering leaf lamina due to presence of loosened cells in its tissues and hyper-accumulation of Ca2+ ions. High accumulation of Ca2+ in PaLCuV infected leaves as compared to healthy leaves which might be the cause of hypertrophy and thickening of veins of infected papaya leaves. Uniform trichomes/hairs/nodular structures were present in midrib of healthy leaf but were missing in infected leaf. The infected midrib showed scantly distributed floret-like structures instead of a smooth trichomes/hairs/nodular structures in midrib of the healthy leaf.
双子叶病毒科木瓜卷叶病毒(PaLCuV)是世界上危害木瓜植物的主要病毒。PaLCuV感染植物的主要视觉特征是向下和向内滚动,叶片呈倒杯状卷曲,叶脉增厚。显微镜观察表明,在健康木瓜叶片中,气孔保卫细胞大小为19-20μm。然而,在高达29-30μm的受感染植物叶片中,它显著增加。这一观察结果表明,气孔密度和保卫细胞大小的变化是由于叶片的褶皱解剖。SEM分析显示,与健康的植物叶片相比,保卫细胞的辅助细胞或附属细胞较少膨胀,淀粉颗粒的排列受到干扰。对症状叶片的光镜、扫描电子显微镜(SEM)和能量色散X射线光谱(EDS)分析显示,由于其组织中存在疏松的细胞和Ca2+离子的过度积累,叶片叶片出现褶皱。与健康叶片相比,PaLCuV感染叶片中Ca2+的高积累可能是感染木瓜叶片静脉肥大和增厚的原因。健康叶片的中脉中存在均匀的毛状体/毛状物/结节状结构,但在感染叶片中缺失。在健康叶片的中脉中,受感染的中脉显示出稀疏分布的小花状结构,而不是光滑的毛状体/毛发/结节状结构。
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引用次数: 1
Effect of foliar boron applications on yield and quality of Medjool date palm 叶面施硼对麦卓枣椰树产量和品质的影响
Q4 Biochemistry, Genetics and Molecular Biology Pub Date : 2018-12-15 DOI: 10.37855/jah.2018.v20i03.32
Hashem S. Al-hajjaj, Jamal Y. Ayad
Effects of foliar boron (B) application as H3BO3 on yield and fruits quality of date palm (Phoenix dactylifera L. var. Medjool) were investigated. The experiment was conducted on 15 years old trees during 2017 growing season. Five different B levels (0, 400, 800, 1200 and 1600 ppm) were applied in a solution of 20 liters∙tree-1 at three equal doses in May, June and July. Fruit yield and quality parameters were determined at maturity harvest stage (tamer). The results showed that foliar B significantly affected yield, bunch weight, fruit set, fruit physicalcharacters and fruit quality. Boron application at the rate of 1600 ppm resulted in 27 % increase in fruits yield (67.7 vs. 53.4 kg∙tree-1), 16 % increase in fruit fresh weight and flesh weight (22.0 vs. 18.9 g.fruit-1), (20.9 vs. 18.0 g fruit-1), respectively, fruit size (49.97 vs. 45.3 mm) and fruit set (88 % vs. 81 %) compared to control. However, the lower rate of boron (400 ppm) was more effective in enhancing fruit quality indices. Total soluble solid (Brix % 75.0), fruit firmness (5.56 kg∙cm-2), glucose content (43.17 %), fructose content (39.28 %) and fruit antioxidant activity (80 %) were significantly increased as compared to control. On other hand, date syrup pH, fruit density and fruit skin separation were not affected by boron application. Overall, the results presented here recommended the use of foliar rates of boron ranging between 400 to 1600 ppm to enhance fruit yield and quality in Medjool dates.
研究了叶面施用硼(B)作为H3BO3对枣椰树(Phoenix dactylifera L. var. Medjool)产量和品质的影响。该实验是在2017年生长季节对15岁的树木进行的。5月、6月和7月在20升∙tree-1溶液中以三次等量施用了五种不同的B水平(0、400、800、1200和1600 ppm)。果实产量和品质参数在成熟期(驯化期)测定。结果表明,叶面B对产量、果重、坐果、果实物理性状和果实品质均有显著影响。施硼量为1600 ppm时,果实产量比对照增加27%(67.7比53.4 kg∙tree-1),鲜重和果肉重分别增加16%(22.0比18.9 g∙fruit-1)、20.9比18.0 g∙fruit-1),果实大小(49.97比45.3 mm)和结实度(88%比81%)。较低硼处理(400ppm)对果实品质指标的改善效果更好。与对照相比,总可溶性固形物(Brix % 75.0)、果实硬度(5.56 kg∙cm-2)、葡萄糖含量(43.17%)、果糖含量(39.28%)和果实抗氧化活性(80%)均显著提高。施用硼对枣浆pH、果实密度和果皮分离度没有影响。综上所述,本研究结果建议叶面硼用量在400 ~ 1600 ppm之间,以提高梅德朱尔枣的产量和品质。
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引用次数: 6
Regulation of chrysanthemum cut flower senescence using 5-sulfosalicylic acid and aluminium sulphate 5-磺基水杨酸和硫酸铝对菊花切花衰老的调控作用
Q4 Biochemistry, Genetics and Molecular Biology Pub Date : 2018-12-15 DOI: 10.37855/jah.2018.v20i03.42
Aparna Veluru, M. Neema, K. Prakash, A. Arora, P. Naveen Kumar, M. Singh
Vase-life is a key factor for evaluating the post-harvest quality of cut flowers that determines their marketability. Chrysanthemum is one of the top most cut flowers sold in international flower markets. In the present study, trials were conducted to improve the postharvest life of chrysanthemum cut flowers using preservative solutions such as 5-sulfosalicylic acid (5-SSA) and aluminium sulphate (Al2(SO4)3) alone or in combination with 1.5 % sucrose. Treatments using 5-SSA (100 ppm and 150 ppm) or 200 ppm Al2(SO4)3 along with 1.5 % sucrose showed a significant increase in vase-life, fresh weight of the cut stems, vase solution uptake, membrane stability index of the petals and leaf chlorophyll as compared to other treatments. Among different vase solutions evaluated, T10 (200 ppm Al2(SO4)3 +1.5 % sucrose) gave maximum vase life of22.3 days, followed by T8 (5-SSA 150 ppm + 1.5 % sucrose) and T6 (100 ppm 5-SSA+ 1.5 % sucrose) treatments with 20.85 and 19.85 days respectively as compared to 17.84 days in control. High concentrations of both the chemicals (5-SSA and Al2(SO4)3) without sucrose showed toxicity symptoms.
花瓶寿命是评价采收后切花质量的关键因素,决定了切花的适销性。菊花是国际花卉市场上最畅销的切花之一。本研究采用5-磺基水杨酸(5-SSA)和硫酸铝(Al2(SO4)3)单独或与1.5%蔗糖联合使用的防腐剂溶液,对菊花切花采后寿命进行了改善试验。与其他处理相比,5-SSA (100 ppm和150 ppm)或200 ppm Al2(SO4)3以及1.5%蔗糖处理显著增加了花瓶寿命、切茎鲜重、花瓶溶液吸收量、花瓣膜稳定性指数和叶片叶绿素。在不同的花瓶溶液中,T10 (200ppm Al2(SO4)3 + 1.5%蔗糖)的最大花瓶寿命为22.3天,其次是T8 (150ppm 5-SSA+ 1.5%蔗糖)和T6 (100ppm 5-SSA+ 1.5%蔗糖)处理,分别为20.85和19.85天,而对照组为17.84天。不含蔗糖的高浓度化学物质(5-SSA和Al2(SO4)3)均表现出毒性症状。
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引用次数: 3
Effects of zinc fertilization on growth and leaf nutrient content of Celosia argentea L. 施锌对青花苜蓿生长和叶片养分含量的影响。
Q4 Biochemistry, Genetics and Molecular Biology Pub Date : 2018-12-15 DOI: 10.37855/jah.2018.v20i03.35
Olukunle Kolade, D. Harp, C. Jones, José G. López
Zinc is an important micronutrient for both plant and human health, and dietary zinc is insufficient in many areas of the world, caused by a diet dominated by grains and vegetables grown on zinc deficient soils. Celosia argentea L. is an herbaceous annual adapted to difficult soil conditions, low soil nutrient content, and hot weather and is commonly grown as a leafy vegetable in many parts of Africa. This study was conducted to evaluate zinc fertilization of celosia at levels higher than normally recommended, but below toxic levels, to determine the effect on plant performance and dietary value. Celosia was grown in a controlled environment with four zinc fertilizer treatments (0, 50, 100 and 150 mg Zn·kg-1). Zinc fertilization had minor effects on plant growth, with differences only found in plant height and root fresh weight with both decreasing at fertilization levels above 100 mg Zn·kg-1. Higher zinc fertilization increased root nitrogen, leaf and root zinc, potassium and magnesium concentrations, though leaf magnesium decreased at the highest zinc fertilizer levels. At 50 mg Zn·kg-1, zinc fertilization toxicity symptoms were unexpessed thus it can improve plant performance and provide potentially significant dietary benefits to people with limited access to zinc in their diet.
锌是植物和人类健康的重要微量营养素,世界许多地区的膳食锌不足,这是由于在缺锌土壤上种植的谷物和蔬菜为主。阿根廷青藤是一种草本一年生植物,适应恶劣的土壤条件、低土壤养分含量和炎热的天气,在非洲许多地区通常作为叶菜种植。本研究旨在评估青藤的锌施肥水平高于正常推荐水平,但低于毒性水平,以确定对植物性能和膳食价值的影响。采用4种锌肥处理(0、50、100和150 mg Zn·kg-1),在控制环境中生长青藤。锌对植株生长的影响较小,仅在株高和根鲜重方面存在差异,当锌浓度超过100mg·kg-1时,两者均呈下降趋势。较高的锌肥增加了根氮、叶和根锌、钾和镁的浓度,但在最高的锌肥水平下,叶镁降低。50 mg Zn·kg-1时,锌的施肥毒性症状没有表现出来,因此它可以改善植物性能,并为饮食中锌摄入量有限的人提供潜在的显著饮食益处。
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引用次数: 1
Micropropagation of Anthurium through suspension culture using in vitro shoots 红掌离体悬浮培养微繁殖研究
Q4 Biochemistry, Genetics and Molecular Biology Pub Date : 2018-12-15 DOI: 10.37855/jah.2018.v20i03.34
Bhavana G.P, B. S. Kumudini, C. Aswath
A simpler and more efficient protocol for mass propagation of Anthurium from somatically derived in vitro shoots through suspension culture was developed. Shoot proliferation was obtained with agar-solidified MS medium as well as liquid MS medium, at various concentration of BAP. After 45 days of incubation at 80 rpm, both the media gave best results at 8.8 μM BAP, whereas higher number (29.333±0.342) of shoots was obtained with liquid medium compared to solid medium (7.667±0.342). The matured plantlets were harvested and subjected to acclimatization after 30 days of primary and 60 days of secondary hardening and then they were transferred to field. Survival rate of plants (71.3 %) in liquid medium was higher compared to the ones from solid medium (43.0 %).
提出了一种更简单、更有效的红掌离体芽悬浮培养批量繁殖方法。在不同浓度的BAP下,琼脂固化的MS培养基和液体MS培养基均可获得芽的增殖。在80 rpm下培养45 d后,两种培养基在8.8 μM BAP下的芽数最高,液体培养基的芽数(29.333±0.342)高于固体培养基(7.667±0.342)。收获成熟植株,经过30 d的初次硬化和60 d的二次硬化后进行驯化,然后移栽田间。液体培养基的成活率为71.3%,高于固体培养基(43.0%)。
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引用次数: 1
Understanding the genetic variability, heritability and association pattern for the characters related to reproductive phase of carrots (Daucus carota L.) in tropical region 了解热带地区胡萝卜(Daucus carota L.)生殖期相关性状的遗传变异、遗传力和关联模式
Q4 Biochemistry, Genetics and Molecular Biology Pub Date : 2018-12-15 DOI: 10.37855/jah.2018.v20i03.39
D. Manikanta, A. P. Chaitra, S. Cholin
Carrot is a sexully propagating, biennial root crop. Flowering related characters and germination parameters have a significant role on the root quality and productivity in the succeeding generations. Larger genetic variation exists in nature for seed and umbel characters due its out crossing behavior. In order to study, the nature of genetic variability, heritability and the association pattern, 48 open pollinated varieties were evaluated in tropical region of Karnataka, India during 2015 for 27 characters including seed, umbel and germination parameters. Both qualitative and quantitative characters were used for the study. The data was subjected to statistical analysis for genetic variability components such as genotypic coefficient of variation (GCV), phenotypic coefficient of variation (PCV), heritability in broad sense (h2) genetic advance (GA) and Genetic advance as percent mean (GAM) and Pearson’s correlation. Genetic variability and heritability coupled with genetic advance as percent mean (GAM) were high for number of umbellates per umbel, bolting tendency and umbel weight. Many other characters showed low to moderate genetic variability coupled with moderate heritability and GAM. From the correlation coefficient analysis few characters viz., density of flowers in umbels, number of umbellates/ umbel, umbel shape, bolting tendency showed positive association with seed and umbel yield. Seed length and size of spines on the seeds affects the germination % negatively. Since, the present study involves maximum characters and good number of cultivars with wider genetic background; hence, detailed understanding of the genetic variability and association pattern for these characters in the reproductive phase of carrot would provide useful information for breeders as well as seed scientists.
胡萝卜是一种无性繁殖的两年生块根作物。开花相关性状和发芽参数对后代根系质量和生产力有重要影响。由于其异交行为,种子和伞形花序性状在自然界中存在较大的遗传变异。为了研究遗传变异性、遗传力和关联模式的性质,2015年在印度卡纳塔克邦热带地区对48个开放授粉品种的27个性状进行了评估,包括种子、伞形花序和发芽参数。研究采用定性和定量相结合的方法。对数据进行遗传变异成分的统计分析,如基因型变异系数(GCV)、表型变异系数(PCV)、广义遗传力(h2)遗传进展(GA)和遗传进展百分比平均值(GAM)和皮尔逊相关性。每个伞形花序的伞形花序数、抽薹倾向和伞形花序重量的遗传变异性和遗传力,加上遗传平均值百分比(GAM)较高。许多其他性状表现出低到中等的遗传变异性,同时具有中等的遗传力和GAM。相关系数分析表明,伞形花序的花密度、伞形花序数、伞形花序形状、抽薹倾向等几个性状与种子和伞形花序产量呈正相关。种子的长度和大小对种子的发芽率有负面影响。鉴于此,本研究涉及最大的性状和大量具有更广泛遗传背景的品种;因此,详细了解胡萝卜繁殖期这些性状的遗传变异和关联模式,将为育种家和种子科学家提供有用的信息。
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引用次数: 1
Assessment of chrysanthemum (Chrysanthemum morifolium Ramat.) germplasm for commercial cultivation under Rayalaseema region of Andhra Pradesh 安得拉邦Rayalaseema地区商业栽培菊花(菊花)种质资源的评估
Q4 Biochemistry, Genetics and Molecular Biology Pub Date : 2018-12-15 DOI: 10.37855/jah.2018.v20i03.37
C. Madhumathi, V. Bhargav, D. Srinivasa Reddy, P. Lalitha Kameshwari, D. Sreedhar, T. Naga Lakshmi
Fifty nine chrysanthemum (Chrysanthemum morifolium Ramat.) genotypes were evaluated for their performance under southern zone of Andhra Pradesh at HRS Farm, Anantharajupeta, Y.S.R. Kadapa, Andhra Pradesh. Significant differences were observed among the genotypes for all vegetative, flowering and yield traits studied. Genotype Selection-5 (56.50 cm) recorded maximum plant height, and plant spread was maximum in two genotypes Chandini and Violet (51.83 cm). Genotypes Sugandha Yellow and Sugandha White produced maximum (11.67) number of primary branches/plant while least in Yellow Cherub (2.33). Earliest bud appearance was observed in genotype Violet (61.67 days), whereas genotype Meera (113.52 days) was late. The highest number of flowers/spray and spray length was produced by genotype Yellow Star and Selection-2 (41.67 and 44.87 cm), respectively. Flower diameter was maximum in Firtation (10.06 cm), however, number of flowers/plant was highest in HYDC7 (287). The weight of single flower and 100 flower weight was maximum in Mother Teresa (4.96 g and 495.50 g). Keeping in view of these characters, genotypes Mother Teresa, Firtation and Chrysanthemum Yellow were found to be highly suitable to grow under Rayalaseema conditions of Andhra Pradesh for loose flower production. Based on hierarchical cluster of the morphological traits, the genotypes were grouped into three. Results of principal component Analysis (PCA) were also in conformity with the results of cluster analysis. Key word: Chrysanthemum, genotype, morphological traits, cluster analysis and PCA
在安得拉邦南部地区的HRS农场,Anantharajupeta,Y.S.R.Kadapa,Andhra Pradesh对59种菊花(菊花)基因型的表现进行了评估。在所研究的所有营养、开花和产量性状的基因型之间都观察到显著差异。基因型选择-5(56.50厘米)记录了最大株高,两个基因型Chandini和Violet(51.83厘米)的植株扩散最大。基因型Sugandha Yellow和Sugandha White主枝/株数最多(11.67),而Yellow Cherub主枝/植株数最少(2.33)。最早出现芽的是基因型Violet(61.67天),而基因型Meera(113.52天)较晚。黄星和Selection-2的花数/喷雾量和喷雾长度最高,分别为41.67和44.87cm。Firtation的花径最大(10.06cm),而HYDC7的花/株数最高(287)。单花重和百花重分别为4.96克和495.50克。鉴于这些特征,发现基因型特蕾莎修女、冷杉和菊花黄非常适合在安得拉邦的Rayalaseema条件下生长,用于散花生产。根据形态性状的层次聚类,将基因型分为三类。主成分分析(PCA)的结果也与聚类分析的结果一致。关键词:菊花,基因型,形态性状,聚类分析和主成分分析
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引用次数: 1
Influence of planting date and plant density on morphological characteristics, seed yield and essential oil percentage of oregano (Origanum vulgare L.) 种植日期和密度对牛至形态特征、种子产量和精油含量的影响
Q4 Biochemistry, Genetics and Molecular Biology Pub Date : 2018-12-15 DOI: 10.37855/jah.2018.v20i03.30
F. Gerami, P. R. Moghaddam, R. Ghorbani, A. Hassani
Over utilization of medicinal plants from wild habitat is causing genetic diversity loss of these valuable plants. In order to determine the optimum planting date and density of oregano for successful production, a field experiment was conducted at the Faculty of Agriculture, Urmia University, Iran. The experimental design was split-plot based on randomized complete blocks with three planting dates (April 19, May 5 and May 20) as main plots and four plant densities (8.33, 10, 12.5 and 16.67 plants m-2) were assigned as sub-plots, all of which replicated three times. Results showed that planting date had significant effects on all traits except the stem proportion and 1000 seed weight. The highest values for all traits were obtained from the first date of planting, whereas values decreased with later planting dates. Also, stem number, plant spread, fresh and dry herb yield, seed yield and essential oil yield significantly influenced by plant density. With increase in plant density, stem number in per plant and plant spread decreased. The maximum fresh and dry herb yield (10618.23 and 2934.36 kg ha-1, respectively) and essential oil yield (42.786 kg ha-1) were obtained at the highest plant density. In conclusion, first planting date with higher plant density caused the greater essential oil yield, fresh and dry herb yield.
对野生栖息地药用植物的过度利用正在导致这些珍贵植物的遗传多样性丧失。为了确定牛至成功生产的最佳种植日期和密度,在伊朗乌尔米亚大学农业学院进行了一项田间试验。实验设计是基于随机完全区块的分裂地块,以三个种植日期(4月19日、5月5日和5月20日)为主地块,四个植物密度(8.33、10、12.5和16.67株m-2)为子地块,所有这些都重复了三次。结果表明,种植日期对除茎秆比例和1000粒重外的所有性状均有显著影响。所有性状的最高值都是从种植的第一天开始获得的,而值随着种植日期的推迟而降低。密度对茎数、株展度、鲜草产量、种子产量和精油产量也有显著影响。随着植株密度的增加,单株茎数和植株展度均呈下降趋势。在最高密度下,鲜草产量和干草产量最高(分别为10618.23和2934.36kg ha-1),精油产量最高(42.786kg ha-1)。综上所述,密度越高,越早定植,精油产量越高,鲜草和干草产量越高。
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引用次数: 1
Growth and yield of apical stem cuttings of white potato (Solanum tuberosum L.) derived from disease-free G0 plants 白马铃薯(Solanum tuberosum L.)无病植株茎尖插条的生长和产量
Q4 Biochemistry, Genetics and Molecular Biology Pub Date : 2018-08-15 DOI: 10.37855/jah.2018.v20i02.25
A. Nikmatullah, I. Ramadhan, M. Sarjan
Growth and production of apical stem cuttings under various soil-less culture conditions for mass production of potato G1 seed tubers was investigated. Different cutting lengths, Indole Acetic Acid (IAA) concentration, and age of mother plants from which the cuttings were taken, were evaluated for survival and growth of the cuttings. In separate experiments, successful cuttings were planted at different spacings and fertilizer rates. The highest survival rate was obtained from apical cuttings with three nodes treated with 1 ppm IAA. The best cutting growth was obtained from cuttings derived from 2 to 3 week-old mother plants. Plant height and individual leaf areas were higher at low spacing (10 cm x 10 cm), and a higher dose of NPK fertilizer (40 g/plot). The number of tubers and tuber weight per plant were higher at a spacing of 20 x 20 cm and fertilizer dose of 40 g/plot. Although higher plant density resulted in smaller tuber size, the combination of close spacing and higher doses of fertilizer resulted in the highest yield per unit area. Importantly, stock plants showed no significant decrease in plant yield after cutting. The results indicate that apical cuttings can be used for the mass-propagation of G0 seed plants to speed up and increase the efficiency of production of G1 seed tubers.
研究了在不同无土栽培条件下马铃薯G1种子块茎顶端茎插条的生长和产量。对不同的扦插长度、吲哚乙酸(IAA)浓度和扦插母株的年龄进行了扦插成活和生长评估。在单独的实验中,成功的插条以不同的间距和施肥率种植。最高的存活率来自用1ppm IAA处理的具有三个节的顶端插条。最佳的扦插生长是从2至3周龄的母株中获得的。在低间距(10 cm x 10 cm)和较高剂量的NPK肥料(40 g/块)下,株高和单株叶面积较高。单株块茎数量和块茎重量在20 x 20厘米的间距和40克/块的肥料剂量下更高。尽管植物密度越高,块茎越小,但紧密的间距和更高剂量的肥料相结合,单位面积产量最高。重要的是,库存植物在切割后没有表现出显著的植物产量下降。结果表明,顶端插穗可用于G0种子植物的大规模繁殖,以加快和提高G1种子块茎的生产效率。
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引用次数: 4
Some natural extracts from plants as low-cost alternatives for synthetic PGRs in rose micropropagation 植物中的一些天然提取物作为玫瑰微繁殖中合成PGRs的低成本替代品
Q4 Biochemistry, Genetics and Molecular Biology Pub Date : 2018-08-15 DOI: 10.37855/jah.2018.v20i02.19
Urmi Chauhan, Anil Kumar Singh, Divyesh Godani, S. Handa, P. Gupta, Shivani Patel, P. Joshi
Effect of various plant extracts during in vitro culture of rose (Rosa hybrida L. cv. bush rose), with the objective of replacing synthetic Plant Growth regulators (PGRs) to reduce the production cost, was studied. Test extracts included sweet lime juice, orange juice, sweet corn extract, tomato fruit extract and coconut water. Significant increase in shoot multiplication (15.41±1.12 shoots/explant), shoot length (3.66±0.08 cm), fresh weight (7.48±0.71 g) and dry weight (1.68±0.075 g) was observed when coconut water (@10 % v/v) was used in the standard MS medium. Addition of tomato fruit extract in the MS medium did not show any noteworthy effect on growth in rose micropropagules. Total chlorophyll and other biomolecules varied with the change in the type and concentration of plant extract. Highest accumulation of biomolecules was recorded on coconut water (@ 10 % v/v) supplemented MS medium followed by sweet corn extract and orange juice. Although tomato fruit extract (@10 % v/v) enhanced the total chlorophyll biosynthesis but at the same time depressed the accumulation of other biomolecules. Treatment of plant extract was given in two different ways; a) incorporation in the medium prior to autoclaving (PrA) and b) post-autoclaving addition of filter sterilized extract (PoA). No significant changes were noted in growth when mode of application was changed. To know the physiological pandemonium in the cells, peroxidase and IAAoxidase activity was noted. No abnormal changes in the activity of these enzymes were recorded in the propagules grown on different plant extracts. The total cost of synthetic 6-benzylaminopurine (BA) can be reduced upto 98 % by replacing it with natural plant extract.
研究了不同植物提取物在玫瑰(Rosa hybrida L.cv.bush rose)体外培养过程中的作用,目的是取代合成植物生长调节剂(PGRs)以降低生产成本。测试提取物包括甜酸橙汁、橙汁、甜玉米提取物、番茄果实提取物和椰子水。当在标准MS培养基中使用椰子水(10%v/v)时,观察到芽增殖(15.41±1.12芽/外植体)、芽长(3.66±0.08cm)、鲜重(7.48±0.71g)和干重(1.68±0.075g)显著增加。在MS培养基中添加番茄果实提取物对玫瑰微繁殖体的生长没有显示出任何显著的影响。总叶绿素和其他生物分子随着植物提取物类型和浓度的变化而变化。生物分子的最高积累记录在椰子水(@10%v/v)补充的MS培养基上,其次是甜玉米提取物和橙汁。尽管番茄果实提取物(@10%v/v)提高了总叶绿素的生物合成,但同时抑制了其他生物分子的积累。以两种不同的方式对植物提取物进行处理;a) 在高压灭菌前掺入培养基中(PrA)和b)高压灭菌后加入过滤灭菌提取物(PoA)。当应用模式改变时,生长没有显著变化。为了了解细胞内的生理混乱,观察了过氧化物酶和IAA氧化酶的活性。在不同植物提取物上生长的繁殖体中,没有记录到这些酶活性的异常变化。合成6-苄基氨基嘌呤(BA)的总成本可以通过用天然植物提取物代替而降低98%。
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引用次数: 3
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Journal of Applied Horticulture
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