Pub Date : 2012-10-18DOI: 10.1109/ENBENG.2012.6331354
S. S. Soares, L. Pedro, G. Ferreira
Protein nanoparticles, such as virus-like particles (VLPs), consist only of the virus shell without any viral genetic information packaged inside. Similarly to viruses, the structural proteins that comprise a VLP can spontaneously self-assemble to form the particle or can assemble through several intermediate steps. This work addresses the characterization, manipulation and purification of a chimeric simian-human immunodeficiency VLP constructed by fusion of SIV matrix protein (p17) and HIV-1 p6 accessory protein. This fusion protein assembles as spherical nanoparticles of about 80 nm in diameter that are released to the culture media when expressed in HEK 293T cells. A simple two-step purification process was used to purify these nanoparticles. Also, different approaches - multiple-transfections or chemical coupling - were performed to target manipulation. Finally, a new approach for the production of these virus-like particles is described where the structural protein subunits are used and their assembly is promoted in vitro.
{"title":"Molecular determinants of virus-like nanoparticle assembly in vitro and in animal cell culture","authors":"S. S. Soares, L. Pedro, G. Ferreira","doi":"10.1109/ENBENG.2012.6331354","DOIUrl":"https://doi.org/10.1109/ENBENG.2012.6331354","url":null,"abstract":"Protein nanoparticles, such as virus-like particles (VLPs), consist only of the virus shell without any viral genetic information packaged inside. Similarly to viruses, the structural proteins that comprise a VLP can spontaneously self-assemble to form the particle or can assemble through several intermediate steps. This work addresses the characterization, manipulation and purification of a chimeric simian-human immunodeficiency VLP constructed by fusion of SIV matrix protein (p17) and HIV-1 p6 accessory protein. This fusion protein assembles as spherical nanoparticles of about 80 nm in diameter that are released to the culture media when expressed in HEK 293T cells. A simple two-step purification process was used to purify these nanoparticles. Also, different approaches - multiple-transfections or chemical coupling - were performed to target manipulation. Finally, a new approach for the production of these virus-like particles is described where the structural protein subunits are used and their assembly is promoted in vitro.","PeriodicalId":399131,"journal":{"name":"2012 IEEE 2nd Portuguese Meeting in Bioengineering (ENBENG)","volume":"11 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2012-10-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"130675551","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2012-10-18DOI: 10.1109/ENBENG.2012.6331370
M. Lopes, T. Scholtz, D. Silva, I. Santos, T. Silva, P. Sampaio, Ricardo M. Couto, V. V. Lopes, C. Calado
An integrated approach for modelling, monitoring and control the plasmid bioproduction in Escherichia coli cultures is presented. In a first stage, by the implementation of a kinetic model for E. coli cultures, a better bioprocess understanding was reached, concerning the availability of nutrients and products along the bioprocess, and their effects on the plasmid production. Results presented may provide significant help for future modelling and monitoring implementation. In a second stage, FTIR spectroscopy coupled with chemometrics, namely PLS regression, shows its potential as a high-throughput technique for simultaneously estimating the key variables involved in the plasmid production process by E. coli cultures run under distinct conditions. Finally, owing to online monitoring and process control, an NIR fibre optic probe and chemometrics provided promising results concerning the control of biomass and carbon sources in E. coli cultures.
{"title":"Modelling, monitoring and control of plasmid bioproduction in Escherichia coli cultures","authors":"M. Lopes, T. Scholtz, D. Silva, I. Santos, T. Silva, P. Sampaio, Ricardo M. Couto, V. V. Lopes, C. Calado","doi":"10.1109/ENBENG.2012.6331370","DOIUrl":"https://doi.org/10.1109/ENBENG.2012.6331370","url":null,"abstract":"An integrated approach for modelling, monitoring and control the plasmid bioproduction in Escherichia coli cultures is presented. In a first stage, by the implementation of a kinetic model for E. coli cultures, a better bioprocess understanding was reached, concerning the availability of nutrients and products along the bioprocess, and their effects on the plasmid production. Results presented may provide significant help for future modelling and monitoring implementation. In a second stage, FTIR spectroscopy coupled with chemometrics, namely PLS regression, shows its potential as a high-throughput technique for simultaneously estimating the key variables involved in the plasmid production process by E. coli cultures run under distinct conditions. Finally, owing to online monitoring and process control, an NIR fibre optic probe and chemometrics provided promising results concerning the control of biomass and carbon sources in E. coli cultures.","PeriodicalId":399131,"journal":{"name":"2012 IEEE 2nd Portuguese Meeting in Bioengineering (ENBENG)","volume":"42 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2012-10-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"130777821","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2012-10-18DOI: 10.1109/ENBENG.2012.6331379
V. Almeida, L. R. Ferreira, C. Correia
The present work focuses an experimental and theoretical approach to non-invasive cardiovascular (CV) system monitoring. The CV diseases are currently one of the leading causes of death in the developed countries. The development of early diagnostic tools contributes for the early identification of important markers in the CV development. A piezoelectric (PZ) probe to arterial pressure waveform (APW) monitoring at peripheral arteries, such as carotid artery was developed. A dedicated test bench capable of emulating the main CV dynamic properties was developed, providing an interesting tool to carry out the preliminary tests. The PZ probe output signal suffers additional processing to original waveform recovering. The deconvolution method was used for this purpose. Algorithms to waveform delineation were developed, based on the wavelet transform and probability density function (PDF). The AIx was calculated for each one of the developed algorithms. These methods were comparatively evaluated with the reference value from the synthesis procedure. The probe's performance was assessed using realistic simulation based on exponential pulses and in in vivo data acquisitions, Results demonstrate that the developed probe exhibits a good performance with maximum errors less than 0.5% in the AIx determination.
{"title":"Hemodynamic parameters assessment","authors":"V. Almeida, L. R. Ferreira, C. Correia","doi":"10.1109/ENBENG.2012.6331379","DOIUrl":"https://doi.org/10.1109/ENBENG.2012.6331379","url":null,"abstract":"The present work focuses an experimental and theoretical approach to non-invasive cardiovascular (CV) system monitoring. The CV diseases are currently one of the leading causes of death in the developed countries. The development of early diagnostic tools contributes for the early identification of important markers in the CV development. A piezoelectric (PZ) probe to arterial pressure waveform (APW) monitoring at peripheral arteries, such as carotid artery was developed. A dedicated test bench capable of emulating the main CV dynamic properties was developed, providing an interesting tool to carry out the preliminary tests. The PZ probe output signal suffers additional processing to original waveform recovering. The deconvolution method was used for this purpose. Algorithms to waveform delineation were developed, based on the wavelet transform and probability density function (PDF). The AIx was calculated for each one of the developed algorithms. These methods were comparatively evaluated with the reference value from the synthesis procedure. The probe's performance was assessed using realistic simulation based on exponential pulses and in in vivo data acquisitions, Results demonstrate that the developed probe exhibits a good performance with maximum errors less than 0.5% in the AIx determination.","PeriodicalId":399131,"journal":{"name":"2012 IEEE 2nd Portuguese Meeting in Bioengineering (ENBENG)","volume":"44 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2012-10-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"130854237","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2012-10-18DOI: 10.1109/ENBENG.2012.6331391
Daniela S. Ferreira, N. Dias
With aging the body and the brain undergoes several changes. One of these changes is the loss of neuroplasticity, which leads to the decrease of cognitive abilities. Hence the necessity of stopping or reversing these changes is of utmost importance to contemporary society. In the present work, using electroencephalogram and the Wisconsin card sorting test (WCST) we try to understand which are the performance-related EEG phenotypes. The test was applied to young and elderly subjects due to the expected cognitive deficits with increasing age. The results showed that parietal theta desynchronization and the increase of the inter-frontal and intra-hemispheric coherence was associated to the group of good performers in the WCST. The implementation of an algorithm for low-resolution brain electromagnetic tomography (LORETA) showed that the WCST is not a specific test of the pre-frontal cortex. The identified performance-related EEG features have potential application on neurofeedback training protocols intended to hinder age-related cognitive decline.
{"title":"Identification of performance-related EEG phenotypes for neurofeedback training","authors":"Daniela S. Ferreira, N. Dias","doi":"10.1109/ENBENG.2012.6331391","DOIUrl":"https://doi.org/10.1109/ENBENG.2012.6331391","url":null,"abstract":"With aging the body and the brain undergoes several changes. One of these changes is the loss of neuroplasticity, which leads to the decrease of cognitive abilities. Hence the necessity of stopping or reversing these changes is of utmost importance to contemporary society. In the present work, using electroencephalogram and the Wisconsin card sorting test (WCST) we try to understand which are the performance-related EEG phenotypes. The test was applied to young and elderly subjects due to the expected cognitive deficits with increasing age. The results showed that parietal theta desynchronization and the increase of the inter-frontal and intra-hemispheric coherence was associated to the group of good performers in the WCST. The implementation of an algorithm for low-resolution brain electromagnetic tomography (LORETA) showed that the WCST is not a specific test of the pre-frontal cortex. The identified performance-related EEG features have potential application on neurofeedback training protocols intended to hinder age-related cognitive decline.","PeriodicalId":399131,"journal":{"name":"2012 IEEE 2nd Portuguese Meeting in Bioengineering (ENBENG)","volume":"13 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2012-10-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"132032980","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2012-10-18DOI: 10.1109/ENBENG.2012.6331397
Isabel Catarina Duarte, F. Janela
The present work aim to evaluate image reconstruction algorithms for Digital Breast Tomosynthesis. Simulated data was used to perform and assess the 3D reconstruction. The images were acquired in a Monte Carlo platform and using an analytical phantom. The reconstruction step was performed, implementing Filtered Back Projection (FBP), Maximum-Likelihood Expectation-Maximization (ML-EM) and Algebraic Reconstruction Technique (ART). Comparable results were obtained by the three algorithms. The FBP algorithm presented more blurring images than the ML-EM and ART algorithms. However, it was the one more capable to localize the structures on the 3D space, including the smallest details. The results of the 3D reconstruction allow the discrimination even of very small structures which could not be differentiated on the simple projections that result from the simulations. This indicates that the accuracy of Digital Breast Tomosynthesis can be better than the Mammography.
{"title":"3D medical image reconstruction on Digital Breast Tomosynthesis","authors":"Isabel Catarina Duarte, F. Janela","doi":"10.1109/ENBENG.2012.6331397","DOIUrl":"https://doi.org/10.1109/ENBENG.2012.6331397","url":null,"abstract":"The present work aim to evaluate image reconstruction algorithms for Digital Breast Tomosynthesis. Simulated data was used to perform and assess the 3D reconstruction. The images were acquired in a Monte Carlo platform and using an analytical phantom. The reconstruction step was performed, implementing Filtered Back Projection (FBP), Maximum-Likelihood Expectation-Maximization (ML-EM) and Algebraic Reconstruction Technique (ART). Comparable results were obtained by the three algorithms. The FBP algorithm presented more blurring images than the ML-EM and ART algorithms. However, it was the one more capable to localize the structures on the 3D space, including the smallest details. The results of the 3D reconstruction allow the discrimination even of very small structures which could not be differentiated on the simple projections that result from the simulations. This indicates that the accuracy of Digital Breast Tomosynthesis can be better than the Mammography.","PeriodicalId":399131,"journal":{"name":"2012 IEEE 2nd Portuguese Meeting in Bioengineering (ENBENG)","volume":"6 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2012-10-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"128289210","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2012-10-18DOI: 10.1109/ENBENG.2012.6331345
L. Mendes, N. Ferreira, C. Comtat
An overview of the multiscale/multiframe (MS/MF) 3D reconstruction scheme for Positron Emission Tomograph is presented. Nowadays, in clinical applications the image is reconstructed using a single scale or grid, i.e., the dimensions of the reconstructed volume or the projection space binning do not change during the reconstruction process. MS/MF introduces the concept of time frame to the multiscale reconstruction proposed by Raheja et al. This approach can be used for the generation of images reconstructed in near real time using a suitable scale, taking full advantage of list-mode reconstruction techniques. The generation of image sequences at different spatial scales and times may be useful to optimize the acquisition protocols on the fly as well as to improve the results of the segmentation and classification algorithms.
{"title":"An overview on the multiscale/multiframe reconstruction for Positron Emission Tomography","authors":"L. Mendes, N. Ferreira, C. Comtat","doi":"10.1109/ENBENG.2012.6331345","DOIUrl":"https://doi.org/10.1109/ENBENG.2012.6331345","url":null,"abstract":"An overview of the multiscale/multiframe (MS/MF) 3D reconstruction scheme for Positron Emission Tomograph is presented. Nowadays, in clinical applications the image is reconstructed using a single scale or grid, i.e., the dimensions of the reconstructed volume or the projection space binning do not change during the reconstruction process. MS/MF introduces the concept of time frame to the multiscale reconstruction proposed by Raheja et al. This approach can be used for the generation of images reconstructed in near real time using a suitable scale, taking full advantage of list-mode reconstruction techniques. The generation of image sequences at different spatial scales and times may be useful to optimize the acquisition protocols on the fly as well as to improve the results of the segmentation and classification algorithms.","PeriodicalId":399131,"journal":{"name":"2012 IEEE 2nd Portuguese Meeting in Bioengineering (ENBENG)","volume":"154 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2012-10-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"116904282","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2012-10-18DOI: 10.1109/ENBENG.2012.6331347
S. Silva, F. Soares, S. Costa, A. Pereira, F. Moreira
The interaction and communication skills are essential to live in society. However, individuals with autism spectrum disorders (ASD) have a gap in these abilities which affects their daily life. Previous studies suggest that children with ASD demonstrate some positive behaviors in presence of a robotic platform. This study intends to evaluate the effect of a robotic platform on children with ASD, checking if the platform can be a stimulating agent for children's interaction, as well as a skill learning promoter. So, it is used the robot Lego Mindstorms NXT as a mediator/reward to encourage children with ASD to interact with others and also to learn some cognitive skills.
{"title":"Development of skills in children with ASD using a robotic platform","authors":"S. Silva, F. Soares, S. Costa, A. Pereira, F. Moreira","doi":"10.1109/ENBENG.2012.6331347","DOIUrl":"https://doi.org/10.1109/ENBENG.2012.6331347","url":null,"abstract":"The interaction and communication skills are essential to live in society. However, individuals with autism spectrum disorders (ASD) have a gap in these abilities which affects their daily life. Previous studies suggest that children with ASD demonstrate some positive behaviors in presence of a robotic platform. This study intends to evaluate the effect of a robotic platform on children with ASD, checking if the platform can be a stimulating agent for children's interaction, as well as a skill learning promoter. So, it is used the robot Lego Mindstorms NXT as a mediator/reward to encourage children with ASD to interact with others and also to learn some cognitive skills.","PeriodicalId":399131,"journal":{"name":"2012 IEEE 2nd Portuguese Meeting in Bioengineering (ENBENG)","volume":"18 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2012-10-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"123940751","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2012-10-18DOI: 10.1109/ENBENG.2012.6331342
R. Campos, E. Figueiras, L. R. Ferreira, A. Humeau-Heurtier
A new multi-wavelength laser Doppler flowmeter prototype with different spaced detection optical fibres, built in order to give depth discrimination capabilities to laser Doppler flowmetry technique (LDF), was developed. The Fourier transform and the power spectral density of LDF signals measured by the laser Doppler flowmeter prototype were calculated in order to analyze the frequency oscillations present in human microcirculatory blood flow and to explore the potential of the prototype for blood flood skin depth discrimination. The spectral dependence of the signals was also calculated using the coherence function. Our results show that perfusion signals from the new prototype present marked differences when wavelengths and fibre separations are modified; these results are found both at rest and during perturbations of local blood flow. These findings support our hypothesis that wide probes and higher wavelengths measure blood flux from deeper layers.
{"title":"Spectral analysis of laser Doppler flowmetry signals","authors":"R. Campos, E. Figueiras, L. R. Ferreira, A. Humeau-Heurtier","doi":"10.1109/ENBENG.2012.6331342","DOIUrl":"https://doi.org/10.1109/ENBENG.2012.6331342","url":null,"abstract":"A new multi-wavelength laser Doppler flowmeter prototype with different spaced detection optical fibres, built in order to give depth discrimination capabilities to laser Doppler flowmetry technique (LDF), was developed. The Fourier transform and the power spectral density of LDF signals measured by the laser Doppler flowmeter prototype were calculated in order to analyze the frequency oscillations present in human microcirculatory blood flow and to explore the potential of the prototype for blood flood skin depth discrimination. The spectral dependence of the signals was also calculated using the coherence function. Our results show that perfusion signals from the new prototype present marked differences when wavelengths and fibre separations are modified; these results are found both at rest and during perturbations of local blood flow. These findings support our hypothesis that wide probes and higher wavelengths measure blood flux from deeper layers.","PeriodicalId":399131,"journal":{"name":"2012 IEEE 2nd Portuguese Meeting in Bioengineering (ENBENG)","volume":"5 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2012-10-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"115097448","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2012-10-18DOI: 10.1109/ENBENG.2012.6331365
S. Coelho, M. C. Pereira, S. Rocha, M. Coelho
Design and testing drug delivery systems using biodegradable and biocompatible colloidal carriers made of functionalized gold nanoparticles are the vectors of this project. The systems will be tested in therapy strategies for cancer. The main objective is to develop nanoparticles that increase radiation absorption in diseased areas and minimize damage to normal surrounding tissues. Such enhancement of radiation will benefit cancer patients by decreasing radiation dose and reducing side effects. Secondary objectives include investigating radiosensitizing potential of gold nanoparticles and selecting most efficient system plus combining nanoparticle-enhanced radiotherapy with anti cancer drugs. The project will contribute to advancing promising delivery systems that can be activated remotely by sensitizing them for light/radiation.
{"title":"Delivery of biomolecules by functionalized inorganic nanoparticles","authors":"S. Coelho, M. C. Pereira, S. Rocha, M. Coelho","doi":"10.1109/ENBENG.2012.6331365","DOIUrl":"https://doi.org/10.1109/ENBENG.2012.6331365","url":null,"abstract":"Design and testing drug delivery systems using biodegradable and biocompatible colloidal carriers made of functionalized gold nanoparticles are the vectors of this project. The systems will be tested in therapy strategies for cancer. The main objective is to develop nanoparticles that increase radiation absorption in diseased areas and minimize damage to normal surrounding tissues. Such enhancement of radiation will benefit cancer patients by decreasing radiation dose and reducing side effects. Secondary objectives include investigating radiosensitizing potential of gold nanoparticles and selecting most efficient system plus combining nanoparticle-enhanced radiotherapy with anti cancer drugs. The project will contribute to advancing promising delivery systems that can be activated remotely by sensitizing them for light/radiation.","PeriodicalId":399131,"journal":{"name":"2012 IEEE 2nd Portuguese Meeting in Bioengineering (ENBENG)","volume":"38 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2012-10-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"115550560","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2012-10-18DOI: 10.1109/ENBENG.2012.6331353
L. M. De Oliveira, M. Barboza
In this paper, cephamycin C purification using column adsorption processes was investigated. Two polymeric resins were used, Q-Sepharose XL, an ionic resin, and Amberlite XAD-4, a neutral one. The column process using QXL separated two fractions with antibacterial activity. Cephamycin C was present only in the first fraction, called F1, but was not pure. In the column process using the resin XAD-4, it was not observed any separation of different compounds when the feed was the ultrafiltered broth. Nevertheless, the system was able to separate two fractions when the feed was the fraction F1. When cephalosporin C was added to F1, the column with XAD-4 also separated cephamycin C from cephalosporin C.
{"title":"Separation of cephalosporins using ionic and neutral polimeric resins","authors":"L. M. De Oliveira, M. Barboza","doi":"10.1109/ENBENG.2012.6331353","DOIUrl":"https://doi.org/10.1109/ENBENG.2012.6331353","url":null,"abstract":"In this paper, cephamycin C purification using column adsorption processes was investigated. Two polymeric resins were used, Q-Sepharose XL, an ionic resin, and Amberlite XAD-4, a neutral one. The column process using QXL separated two fractions with antibacterial activity. Cephamycin C was present only in the first fraction, called F1, but was not pure. In the column process using the resin XAD-4, it was not observed any separation of different compounds when the feed was the ultrafiltered broth. Nevertheless, the system was able to separate two fractions when the feed was the fraction F1. When cephalosporin C was added to F1, the column with XAD-4 also separated cephamycin C from cephalosporin C.","PeriodicalId":399131,"journal":{"name":"2012 IEEE 2nd Portuguese Meeting in Bioengineering (ENBENG)","volume":"130 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2012-10-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"128618554","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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