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2012 IEEE 2nd Portuguese Meeting in Bioengineering (ENBENG)最新文献

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Novel approach to vascular network modeling in 3D 血管网络三维建模的新方法
Pub Date : 2012-10-18 DOI: 10.1109/ENBENG.2012.6331381
Maria Margarida Dias Soares Quinas Guerra, Rui D. M. Travasso
A simulation of a hybrid multi-scale phase-field model which describes the dynamics of the interface that divides the new capillaries and the stroma was performed. Four equations model the behaviour of proliferating endothelial cells (ECs) in response to a gradient of a pro-angiogenic factor (T), leading to the growth and time evolution of a tree of vascular vessels from sprouts emerging from the original capillar. New sprouts are pulled by activated tip cells and move with a velocity proportional to the gradient of angiogenic factors. The sources of pro-angiogenic factor are hypoxic cells. This factor diffuses throughout a three-dimensional system and is consumed by the ECs whose proliferation is governed by an order parameter (φ). Higher values of chemotactic response led to an increase of the number of ramifications in the vessels, which were thinner; higher values of proliferation rate to thicker vessels and a more ramified network and an increase in the number of hypoxic cells led to a higher number of branches, higher angiogenic factor consumption, longer and internally more proliferative vessels.
模拟了一个混合多尺度相场模型,该模型描述了划分新毛细血管和基质的界面动力学。四个方程模拟了增殖内皮细胞(ECs)对促血管生成因子(T)梯度的响应行为,导致从原始毛细血管中萌芽的血管树的生长和时间进化。新的芽被激活的尖端细胞拉着,并以与血管生成因子梯度成正比的速度移动。促血管生成因子的来源是缺氧细胞。这个因子在整个三维系统中扩散,并被ec消耗,ec的扩散由一个序参数(φ)控制。趋化反应越高,血管分支数量增加,血管变薄;越厚的血管和分支网络的增殖率越高,缺氧细胞数量的增加导致分支数量越多,血管生成因子消耗越多,血管越长,内部的增殖能力越强。
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引用次数: 6
Biosensor platform for transcription factor DNA binding activity detection 转录因子DNA结合活性检测的生物传感器平台
Pub Date : 2012-10-18 DOI: 10.1109/ENBENG.2012.6331361
B. Tomé, R. Rodrigues, G. Ferreira
The general objective of this project is to develop an acoustic biosensor platform to study protein-DNA binding and its application to the study of DNA transcription factors. An impedance analysis methodology enables the calculation of the acoustic energy dissipation per unit mass observed upon DNA binding, providing quantitative information on the size and shape of the tethered molecules. The rationale of the project rely on the fact that the conformational changes and bending of DNA upon protein binding increase the rigidity of DNA films immobilized at the surface of an acoustic sensor. As a result less acoustic energy is dissipated what is signaled by a decrease of the variation of the acoustic motional resistance. Such experimental approach, together with the associated mathematical signal processing and physical modeling, will provide quantitative information on sequence and conformation DNA sequences recognized by specific transcriptional factors as well as affinity and kinetic constants.
本项目的总体目标是开发一个声学生物传感器平台来研究蛋白质-DNA结合及其在DNA转录因子研究中的应用。阻抗分析方法可以计算DNA结合时观察到的每单位质量的声能量耗散,从而提供有关拴链分子大小和形状的定量信息。该项目的基本原理依赖于这样一个事实,即蛋白质结合时DNA的构象变化和弯曲增加了固定在声学传感器表面的DNA膜的刚性。因此,较少的声能被耗散,这是由声运动阻力变化的减少所发出的信号。这种实验方法,连同相关的数学信号处理和物理建模,将提供由特定转录因子识别的序列和构象DNA序列的定量信息,以及亲和和动力学常数。
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引用次数: 0
A modified Hilbert-Huang algorithm to the assessment of heart rate variability 一种改进的Hilbert-Huang算法评估心率变异性
Pub Date : 2012-10-18 DOI: 10.1109/ENBENG.2012.6331355
C. Tavares, R. Martins, M. Oliveira, S. Laranjo, I. Rocha
Heart rate variability is a classical method to quantify the autonomic outflow to the cardiovascular system. The current methods in frequency and time-frequency domains have shown some limitations in resolution. In this paper, we propose a modified Hilbert-Huang algorithm to the assessment of autonomic activity in heart rate signals. These approaches are evaluated while analyzing both simulated signals and experimental data from tachograms associated with cardiac autonomic blockade of patients with paroxysmal atrial fibrillation.
心率变异性是量化流向心血管系统的自主神经流出的经典方法。现有的频域和时频域方法在分辨率上存在一定的局限性。在本文中,我们提出了一种改进的Hilbert-Huang算法来评估心率信号中的自主活动。通过分析与阵发性心房颤动患者心脏自主神经阻滞相关的声像图的模拟信号和实验数据,对这些方法进行了评估。
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引用次数: 1
FLIM as a tool for metabolic imaging of the cornea FLIM作为角膜代谢成像的工具
Pub Date : 2012-10-18 DOI: 10.1109/ENBENG.2012.6331350
A. Batista, C. Loureiro, J. Domingues, J. S. Silva, M. Morgado
We intend to develop an efficient method of measuring respiratory function of the cornea. With this purpose, we resorted to fluorescence lifetime imaging microscopy (FLIM) to monitor the metabolic co-factor flavin adenine dinucleotide (FAD). FAD and nicotinamide adenine dinucleotide (NADH) are co-factors of the electron transport chain. Therefore alterations in amount of these molecules reflect alterations in the metabolism. For assessing the potential of FLIM for metabolic imaging of the cornea, we performed a series of experiments using a time-correlated single photon counting (TCSPC) fluorescence lifetime microscope (Picoquant to MicroTime 100 coupled to an Olympus BX51 Microscope). In this technique, the acquired signal is the convolution between the instrument response function (IRF) and the fluorescence signal from the sample. IRF was acquired using an Erythrosin B solution. In this work we show that it is possible to acquire fluorescence lifetime images of rat and bovine corneas using FAD autofluorescence.
我们打算发展一种有效的测量角膜呼吸功能的方法。为此,我们采用荧光寿命成像显微镜(FLIM)监测代谢辅助因子黄素腺嘌呤二核苷酸(FAD)。FAD和烟酰胺腺嘌呤二核苷酸(NADH)是电子传递链的辅助因子。因此,这些分子数量的变化反映了代谢的变化。为了评估FLIM在角膜代谢成像中的潜力,我们使用时间相关单光子计数(TCSPC)荧光寿命显微镜(Picoquant to MicroTime 100与Olympus BX51显微镜耦合)进行了一系列实验。在该技术中,采集的信号是仪器响应函数(IRF)与样品荧光信号之间的卷积。IRF用红素B溶液获得。在这项工作中,我们表明,它是可能获得荧光寿命图像的大鼠和牛角膜使用FAD自体荧光。
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引用次数: 0
Preliminary characterization of the external proton beam from a PET cyclotron for use in neutron and proton radiobiology and other dosimetric studies 用于中子和质子放射生物学和其他剂量学研究的PET回旋加速器外部质子束的初步表征
Pub Date : 2012-10-18 DOI: 10.1109/ENBENG.2012.6331362
S. Ghithan, H. Simões, F. Alves, S. Carmo, M. Cunha, R. Marques, F. Fraga, M. Pinto, P. Crespo
Different cyclotron models capable of accelerating protons up to 20MeV have been worldwide installed. Although their purpose is mainly positron emission tomography (PET) radioisotope production, they are equipped with several beam lines suitable for scientific research. Each beam line may typically deliver proton currents up to 150 μA (1×1015 particles/s). Radiobiological and dosimetric studies can be performed using these beam lines, which may contribute to further improve ion therapy and material radiation hardness results, among other applications. We report experimental results aiming at characterizing the proton beam achievable outside a PET cyclotron. In addition, we simulate this experimental setup by using Geant4. We show that simulation is consistent with previous published experimental data and with our first-measured results. These point to a beam angular spreading which may be utilized to establish an irradiation setup within the bunker. We estimate that the dose achievable with such setup may span 4-orders-of-magnitude, useful in radiobiology, ranging from 10mGy to 100 Gy. Finally, we show by simulation that neutron and γ-ray dose on a realistic, in-bunker target is negligible down to at most the 1% level. Further quantification for lower levels is ongoing.
世界各地已经安装了不同的回旋加速器模型,能够将质子加速到20MeV。虽然它们的目的主要是生产正电子发射断层扫描(PET)放射性同位素,但它们配备了几条适合科学研究的束流线。每个束流线通常可以提供高达150 μA (1×1015粒子/秒)的质子电流。使用这些束流线可以进行放射生物学和剂量学研究,这可能有助于进一步改善离子治疗和材料辐射硬度结果,以及其他应用。我们报告了旨在表征PET回旋加速器外可实现的质子束的实验结果。此外,我们还使用Geant4模拟了这个实验设置。我们表明,模拟与先前发表的实验数据和我们的首次测量结果是一致的。这些指向一束角扩散,可用于在掩体内建立辐照装置。我们估计,这种装置可达到的剂量可能跨越4个数量级,在放射生物学中有用,范围从10mGy到100gy。最后,我们通过模拟表明,中子和γ射线剂量对一个现实的,在掩体内的目标是可以忽略不计的,最高可达1%的水平。对较低水平的进一步量化正在进行中。
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引用次数: 7
The effect of methamphetamine on subventricular zone neurogenesis: Cell death, proliferation and differentiation 甲基苯丙胺对脑室下区神经发生的影响:细胞死亡、增殖和分化
Pub Date : 2012-10-18 DOI: 10.1109/ENBENG.2012.6331378
A. R. Bento, S. Baptista, J. Malva, A. P. Silva, F. Agasse
Methamphetamine (METH) is a potent and widely consumed psychostimulant which causes brain functional and structural abnormalities. However, little is known about the effect of METH on adult neurogenic niches and its consequences on the subventricular zone (SVZ). Thus, this work aims to disclose the effects of METH on SVZ neurogenesis. SVZ neurospheres were cultured from early postnatal mice and subjected to increasing concentrations of METH (1 μM to 500 μM). After 24 hours of exposure to METH cell death was triggered by both necrosis and apoptosis. METH exerted toxic effects on stem/progenitor cells expressing SOX2, but not on doublecortin-labeled neuroblasts. METH decreased BrdU incorporation in SVZ cell cultures. Furthermore, METH decreased the number of NeuN-positive neurons, as well as P-JNK-positive growing axons. Altogether, our data demonstrate that METH is toxic to SVZ cells and reduces neuronal differentiation and maturation at non toxic concentrations.
甲基苯丙胺是一种广泛使用的强效精神兴奋剂,可导致大脑功能和结构异常。然而,甲基安非他明对成人神经源性壁龛的影响及其对心室下区(SVZ)的影响知之甚少。因此,本研究旨在揭示甲基安非他明对SVZ神经发生的影响。从出生后早期小鼠中培养SVZ神经球,并增加甲基安非他明浓度(1 μM至500 μM)。暴露于甲基安非他明24小时后,细胞以坏死和凋亡两种方式死亡。甲基苯丙胺对表达SOX2的干细胞/祖细胞有毒性作用,但对双皮质素标记的神经母细胞没有毒性作用。甲基安非他明降低SVZ细胞培养中BrdU的掺入。此外,甲基安非他明减少了neun阳性神经元的数量,以及p - jnk阳性生长轴突的数量。总之,我们的数据表明,甲基安非他明对SVZ细胞是有毒的,并且在无毒浓度下会减少神经元的分化和成熟。
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引用次数: 0
Development and characterization of new sensors for hemodynamic evaluation: Fibre Bragg sensor for arterial pulse waveform acquisition 用于血流动力学评估的新型传感器的开发和特性:用于动脉脉冲波形采集的光纤布拉格传感器
Pub Date : 2012-10-18 DOI: 10.1109/ENBENG.2012.6331343
C. Leitão, H. Lima, J. Pinto, L. Bilro, P. Antunes, C. Marques, J. Prata, P. André, R. Nogueira
Cardiovascular diseases are the leading cause of death in the world, arising their early prediction as a key aspect. The analysis of pulse wave propagation characteristics, such as velocity and morphology, can give important data about arterial stiffness, a parameter with growing significance in cardiovascular events prediction. In this paper it is presented a new optical fibre sensor to assess the pressure waveform in the carotid artery. The optical sensor is based on fibre Bragg grating technology, increasingly applied in several fields, including biomedical sensing due to its advantageous features. The Bragg sensor was characterized and tested in human carotids. It is presented a case study showing the great potential of Bragg technology in the assessment of the pressure waveform.
心血管疾病是世界上导致死亡的主要原因,其早期预测是一个关键方面。分析脉搏波的传播特性,如速度和形态,可以提供动脉刚度的重要数据,这是一个在心血管事件预测中越来越重要的参数。本文提出了一种新型的用于颈动脉压力波形测量的光纤传感器。基于光纤光栅技术的光学传感器,由于其优越的特性,越来越多地应用于包括生物医学传感在内的多个领域。Bragg传感器在人颈动脉中进行了表征和测试。本文给出了一个案例研究,展示了Bragg技术在压力波形评估中的巨大潜力。
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引用次数: 2
3D automatic lung segmentation in low-dose CT 低剂量CT三维肺自动分割
Pub Date : 2012-10-18 DOI: 10.1109/ENBENG.2012.6331360
F. Nery, J. S. Silva, N. Ferreira, F. Caramelo
The amount of information generated by medical imaging procedures as well as the number of exams performed all over the world is increasing over time. This leads to the need of faster and more efficient ways to deal with the large datasets characteristic of these procedures. Computer-aided diagnostic methods have an important role in this area. This paper presents a fully automatic method for the identification of the lungs in CT images. The lung regions are identified by a threshold operation as a first step. To separate merged lungs, we apply a sequence of morphological operations. Additionally the trachea and large airways are identified and removed in each slice. The proposed approach was tested in several whole-body CT studies presenting positive results.
随着时间的推移,医学成像程序产生的信息量以及在世界各地进行的检查数量都在增加。这导致需要更快和更有效的方法来处理这些程序的大数据集特征。计算机辅助诊断方法在这一领域发挥着重要作用。本文提出了一种用于CT图像中肺的全自动识别方法。作为第一步,通过阈值操作识别肺区域。为了分离合并的肺,我们采用一系列形态学手术。此外,在每个切片上识别并切除气管和大气道。该方法在几项全身CT研究中进行了测试,显示出积极的结果。
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引用次数: 5
Spectrophotometric approach for automatic human blood typing 自动人血型分型的分光光度法
Pub Date : 2012-10-18 DOI: 10.1109/ENBENG.2012.6331340
S. Pimenta, G. Minas, F. Soares
The research team is working in the development of automatic and miniaturized devices for medical applications. An example of this work is the development of a miniaturized, low cost, portable and automatic system to blood typing in emergency situations, based on a spectrophotometric approach and in the presence of agglutination (interaction between red blood cells' surface and specific reagents). The application of a simple and fast experimental protocol allows determining blood typing and enables the design of an electronic automatic system. This system will be useful to reduce some limitations of the existing systems and methods to blood typing.
该研究小组正致力于开发用于医疗应用的自动化和小型化设备。这项工作的一个例子是基于分光光度法和存在凝集(红细胞表面与特定试剂之间的相互作用)的情况下,在紧急情况下开发一种小型化、低成本、便携式和自动的血型系统。应用简单快速的实验方案可以确定血型,并使电子自动系统的设计成为可能。该系统将有助于减少现有系统和方法对血型的一些限制。
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引用次数: 8
Retinal imaging with photoreceptor resolution 视网膜成像与光感受器分辨率
Pub Date : 2012-10-18 DOI: 10.1109/ENBENG.2012.6331352
S. Nascimento, H. T. Correia, Sandra Franco, Jose Manuel Borges de Ameida
Retinal imaging capable of resolving individual photoreceptors cannot be obtained with conventional ophthalmoscopy but requires compensation of the individual optical aberrations that blur the optical image from the fundus. This optical compensation is normally accomplished by adaptive optics. We built a retinal imaging system based on adaptive optics can image individual cone photoreceptors. The system uses infrared radiation from a superluminescent diode (SLD) and a Hartman-Shack wavefront sensor to measure eye aberrations. An ophthalmoscope channel with a digital camera is integrated with the system to visualize the retina. A deformable mirror is used to partially compensate the aberrations of the light from the eye thereby improving the image quality and optical resolution. Images from the fundus were obtained from one healthy retina at about 0.5-1.0 deg eccentricity. The cone photoreceptor mosaic is visible and individual photoreceptors can be inspected.
能够分辨单个光感受器的视网膜成像不能通过传统的眼科检查获得,但需要补偿从眼底模糊光学图像的单个光学像差。这种光学补偿通常由自适应光学来完成。我们建立了一个基于自适应光学的视网膜成像系统,可以对单个视锥光感受器进行成像。该系统使用来自超发光二极管(SLD)和哈特曼-沙克波前传感器的红外辐射来测量眼像差。带有数码相机的检眼镜通道与该系统集成,以可视化视网膜。可变形反射镜用于部分补偿来自眼睛的光的像差,从而提高图像质量和光学分辨率。眼底图像取自一个健康视网膜,偏心率约0.5-1.0度。视锥光感受器马赛克可见,单个光感受器可以检查。
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引用次数: 0
期刊
2012 IEEE 2nd Portuguese Meeting in Bioengineering (ENBENG)
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