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Assessment of Cartilage Oligomeric Matrix Protein Biomarker for Laboratory Diagnosis of Hepatocellular Carcinoma 评估用于肝细胞癌实验室诊断的软骨寡聚基质蛋白生物标记物
Pub Date : 2024-06-08 DOI: 10.21608/jmals.2024.288820.1024
O. Othman
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引用次数: 0
Isolation and Identification of some fresh Protozoa from surface water sources and taps in Dakahlyia Governorate, Egypt 埃及达卡利亚省地表水源和水龙头中一些新鲜原生动物的分离与鉴定
Pub Date : 2024-06-01 DOI: 10.21608/jmals.2024.350552
Samir El-Abbassy, Enayat Reda, Asmaa Marzouk
The River Nile represents the main source of water in Egypt, used for irrigation, drinking, and fisheries. Therefore, it is so necessary to undergo frequent monitoring of the protozoan biodiversity in fresh water to avoid epidemic diseases. Accordingly, this work aims to isolate and identify the expected protozoan species in water samples collected from the River Nile and taps at four sites in Dakahlia Governorate. Water samples were collected using the concentration technique, followed by modified Ziel-Neelsen stain and Giemsa staining methods for detecting protozoan species using bright light and phase-contrast microscopy. 12 protozoan species belonging to the subphylum Sarcodina, Masigophora, and Cilliophora were recorded. Moreover, examination of water samples revealed that drinking water was contaminated by six species of protozoa. A description of the locomotive and cyst stages is carried out for most of the detected protozoa. Conclusion: This study proved that drinking water must be carefully analyzed to avoid the presence or growth of protozoa
尼罗河是埃及的主要水源,用于灌溉、饮用和渔业。因此,有必要经常监测淡水中的原生动物生物多样性,以避免流行性疾病的发生。因此,这项工作旨在分离和鉴定从尼罗河和达卡利亚省四个地点的水龙头采集的水样中的预期原生动物物种。采集水样时使用了浓缩技术,然后使用改良的 Ziel-Neelsen 染色法和 Giemsa 染色法,利用强光和相衬显微镜检测原生动物种类。结果记录了 12 种原生动物,分别属于 Sarcodina、Masigophora 和 Cilliophora 亚门。此外,对水样的检测显示,饮用水受到了 6 种原生动物的污染。对大多数检测到的原生动物的胎生阶段和囊状阶段进行了描述。结论这项研究证明,必须对饮用水进行仔细分析,以避免原生动物的存在或生长。
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引用次数: 0
IMMUNOHISTOCHEMICAL EXPRESSION OF ESTROGEN RECEPTORS AS PROGNOSTIC MARKER AND CORRELATION WITH CLINICOPATHOLOGICAL PARAMETERS IN HUMAN ENDOMETRIAL ADENOCARCINOMA 人子宫内膜腺癌中作为预后标志的雌激素受体的免疫组化表达及其与临床病理参数的相关性
Pub Date : 2024-06-01 DOI: 10.21608/jmals.2024.350487
Saeed Mohamed, A. Amin, Aisha Salih, S. Al-Qadasi, R. Sultan
In human steroid-sensitive tissue, Estrogen receptors (ERs) have been demonstrated at the mRNA and/or protein levels by using molecular biology and immunohistochemistry techniques. Estrogen receptors are present in endometrial hyperplasia and neoplastic endometrium. The present work aimed to compare the expression of ER as a prognostic biomarker in human endometrial adenocarcinoma versus benign tumors and normal endometrial tissues as well as their correlation with different pathological and histological parameters. The immunohistochemical technique was used to examine the expression of ER in normal, benign as well as in endometrial adenocarcinoma. Present results showed higher expression of ER in endometrial adenocarcinoma compared to normal and benign endometrial tissues.
通过分子生物学和免疫组化技术,雌激素受体(ER)已在人类类固醇敏感组织的 mRNA 和/或蛋白质水平上得到证实。雌激素受体存在于子宫内膜增生和肿瘤性子宫内膜中。本研究旨在比较作为预后生物标志物的ER在人类子宫内膜腺癌、良性肿瘤和正常子宫内膜组织中的表达情况,以及它们与不同病理和组织学参数的相关性。免疫组化技术用于检测ER在正常、良性和子宫内膜腺癌中的表达。研究结果表明,与正常和良性子宫内膜组织相比,ER在子宫内膜腺癌中的表达更高。
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引用次数: 0
The relationship between Celiac Disease (CD) and obesity: A Review 乳糜泻(CD)与肥胖之间的关系:综述
Pub Date : 2024-06-01 DOI: 10.21608/jmals.2024.358399
Allaa Jamel Shhaet, Muhanad Mahdi Mohammed
,
,
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引用次数: 0
Annona muricata leaves extract mitigates the testicular oxidative stress induced by doxorubicin in male rats 牡丹叶提取物可减轻多柔比星对雄性大鼠睾丸的氧化应激作用
Pub Date : 2024-06-01 DOI: 10.21608/jmals.2024.349721
Rania Abou-El-magd, Olfat Elghareeb, Hanem El-sherbiny, Najamul Nisa
Background: Doxorubicin (DOX) is a strong antineoplastic drug that is used for the treatment of several types of cancer and has toxic effects, Annona muricata fruits and leaves possess phytotherapeutic bioactive constituents have potency in the treatment of several diseases. Aim: This study aims to evaluate the therapeutic role of Annona muricata extract (AME) against the testicular damage induced by doxorubicin in male rats. Material &methods: Thirty-two male adult albino rats were used in this study. The rats were divided into four groups (n = 8): the control group, the AME-supplemented group (100 mg/kg b.wt. daily for consecutive 28 days), the DOX-treated group (2 mg/kg twice a week for 4 weeks), and the DOX-treated group that was supplemented with AME. Results : Treatment of male rats with DOX showed a remarkable increase in the levels of serum pro-inflammatory markers, decreased sexual hormones, and decreased testicular antioxidant activities. The treatment of DOX-injected rats with ASE revealed a pronounced return to normalcy of the oxidative stress markers, as evidenced by a remarkable decrease in the testicular MDA and an improvement of SOD, CAT, and GSH activities. On the other hand, the rats administered DOX and AME had considerably decreased serum levels of TNF-α and IL-6 and improved serum FSH, LH, and testosterone levels. AME successfully restored the testicular histopathological signs induced by DOX through amelioration of the architecture of disrupted seminiferous tubules with AME. Conclusion: Annon muricata extract has a potential alleviation role against DOX-induced oxidative stress and inflammation in the testicular tissues.
背景:多柔比星(Doxorubicin,DOX)是一种强效抗肿瘤药物,用于治疗多种类型的癌症,具有毒性作用。目的:本研究旨在评估鼠李子提取物(AME)对雄性大鼠多柔比星引起的睾丸损伤的治疗作用。材料与方法:本研究使用了32只成年雄性白化大鼠。大鼠分为四组(n = 8):对照组、添加AME组(每天100毫克/千克体重,连续28天)、DOX治疗组(每周2次,每次2毫克/千克,连续4周)和DOX治疗后添加AME组。结果:雄性大鼠在接受DOX治疗后,血清中的促炎标志物水平显著升高,性激素水平下降,睾丸抗氧化活性降低。用 ASE 治疗注射 DOX 的雄性大鼠后,氧化应激指标明显恢复正常,表现为睾丸 MDA 显著下降,SOD、CAT 和 GSH 活性提高。另一方面,服用DOX和AME的大鼠血清中的TNF-α和IL-6水平显著下降,血清中的FSH、LH和睾酮水平有所提高。AME通过改善被破坏的曲细精管的结构,成功恢复了DOX诱发的睾丸组织病理学症状。结论鼠尾草提取物对DOX诱导的睾丸组织氧化应激和炎症具有潜在的缓解作用。
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引用次数: 0
The possibility of using Culex pipiens (Diptera: Culicidae) larvae as a bioindicator of water pollution in Burullus Lake, Egypt 利用库蚊(双翅目:库蚊科)幼虫作为埃及 Burullus 湖水污染生物指标的可能性
Pub Date : 2024-06-01 DOI: 10.21608/jmals.2024.351977
Noura Toto, Shimaa S. Khattab, Samir El-Abbassy, Salwa A. El-Saidy
The urgent environmental issue of heavy metal pollution in aquatic environments has brought attention to Burullus Lake due to its environmental and economic significance. Therefore, this study focused on using the third-instar larvae of Culex pipiens as bioindicators of metal pollution in two Burullus Lake sites: Al-Bughaz (site 1, reference) and the front of Drain 7 (site 2, polluted). The water physicochemical analysis confirmed site 2's higher pollution levels. Metal concentrations in water and larval midgut tissues from site 2 were significantly higher than in site 1. All biochemical parameters estimated in site 2's larval midgut tissues showed greater impacts compared to site 1. Concentration levels of malondialdehyde and metallothionein along with expression level of heat shock protein 70 were notably higher in site 2's larvae. Antioxidant biological markers, like reduced glutathione, superoxide dismutase, catalase, and glutathione peroxidase, as well as cytochrome P450 activity decreased significantly in site 2's larvae compared to the reference larvae from site 1. Analysis of DNA damage parameters in the midgut cells of site 2's larvae revealed significant rises in tail DNA%, tail length, and tail moment. DNA damage was increased in site 2's larvae due to migration of DNA fragments. Ultrastructure observations of site 2's larvae revealed structural alterations along the epithelial cells of midgut tissue like destroyed microvilli, an irregular nuclear envelope, vacuolated cytoplasm, increasing lysosomal bodies, disintegrated mitochondria, and the appearance of spherites and dense vesicles. In conclusion, C. pipiens larvae are reliable bioindicators for metal pollution in aquatic environments due to their sensitivity to metal bioaccumulation.
由于布拉鲁斯湖在环境和经济方面的重要意义,水生环境中重金属污染这一紧迫的环境问题引起了人们的关注。因此,本研究重点利用库蚊三龄幼虫作为金属污染的生物指标:Al-Bughaz(地点 1,参考)和 7 号排水沟前(地点 2,污染)。水理化分析证实 2 号污染点的污染程度较高。2 号地点的水和幼虫中肠组织中的金属浓度明显高于 1 号地点。与污染点 1 相比,污染点 2 幼虫中肠组织中的所有生化参数都受到了更大的影响。2号地点的幼虫体内丙二醛和金属硫蛋白的浓度水平以及热休克蛋白70的表达水平明显更高。与 1 号参照区的幼虫相比,2 号参照区幼虫的抗氧化生物标志物,如还原型谷胱甘肽、超氧化物歧化酶、过氧化氢酶、谷胱甘肽过氧化物酶以及细胞色素 P450 活性显著降低。对2号地点幼虫中肠细胞的DNA损伤参数进行分析后发现,尾部DNA%、尾长和尾矩都明显增加。由于DNA片段的迁移,2号地点幼虫的DNA损伤增加。2 号地点幼虫的超微结构观察结果显示,中肠组织上皮细胞的结构发生了改变,如微绒毛被破坏、核包膜不规则、细胞质空泡化、溶酶体增多、线粒体解体、出现球粒和致密小泡。总之,由于琵琶鱼幼虫对金属生物蓄积的敏感性,它们是水生环境中金属污染的可靠生物指标。
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引用次数: 0
Combination Therapy with Vitamin D and Telmisartan to Suppress the Progression of Liver Fibrosis. 维生素 D 和替米沙坦联合疗法可抑制肝纤维化的进展
Pub Date : 2024-06-01 DOI: 10.21608/jmals.2024.278909.1020
Fatma Amer, Mahmoud El-rehany, Othman Othman
Hepatic fibrosis is an improper wound repair response associated with various kinds of chronic liver injuries. It can be detected by over-deposition of diffuse ECM (Extracellular Matrix) and abnormal overgrowth of connective tissue, and it can progress to cirrhosis or hepatocellular carcinoma. Chronic liver diseases, which include liver fibrosis, have resulted in significant morbidity and mortality worldwide, and this trend is likely to continue. Although early liver fibrosis has been reported to be reversible, the exact mechanism of reversal is unidentified, and there is a lack of effective therapy for liver fibrosis. Vitamin D is essential for maintaining the metabolism of bone and calcium balance. Unexpectedly new evidence indicates that vitamin D has a protective function toward hepatic fibrosis. Telmisartan has been shown to have a positive effect on hepatic fibrosis markers in various pathological contexts. This review focused on summarizing the effect of vitamin D and telmisartan on the progression of hepatic fibrosis and updating their present impact on fibrosis. Telmisartan and vitamin D reduce fibrosis in hepatic stellate cells by inhibiting the production of profibrogenic genes.
肝纤维化是与各种慢性肝损伤相关的一种不正常的伤口修复反应。肝纤维化可通过弥漫性 ECM(细胞外基质)过度沉积和结缔组织异常增生而发现,并可发展为肝硬化或肝细胞癌。包括肝纤维化在内的慢性肝病已在全球范围内造成了严重的发病率和死亡率,而且这种趋势很可能会继续下去。虽然有报道称早期肝纤维化是可以逆转的,但逆转的确切机制尚不明确,而且肝纤维化也缺乏有效的治疗方法。维生素 D 是维持骨骼代谢和钙平衡所必需的。意外的是,新证据表明维生素 D 对肝纤维化具有保护作用。在各种病理情况下,替米沙坦对肝纤维化标志物有积极影响。本综述重点总结了维生素D和替米沙坦对肝纤维化进展的影响,并更新了它们目前对肝纤维化的影响。替米沙坦和维生素D通过抑制嗜碱性基因的产生来减轻肝星状细胞的纤维化。
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引用次数: 0
COMPARATIVE STUDY OF IMMUNOHISTOCHEMICAL EXPRESSION OF GP88 AND Cath-D AS PROGNOSTIC MARKERS AND CORRELATION WITH PATHOLOGICAL AND HISTOLOGICAL PARAMETERS IN HUMAN BREAST CANCER 人类乳腺癌中作为预后标志的 GP88 和 Cath-D 的免疫组织化学表达及其与病理和病史参数的相关性比较研究
Pub Date : 2024-04-08 DOI: 10.21608/jmals.2024.349436
S. Al-Qadasi, M. Yahia, SaeedSamah Mohamed, R. Sultan
Progranulin or acrogranin is an 88-kDa glycoprotein identified by a biological screen for protein targets associated with high tumorigenicity. Cathepsin D (Cath-D) is a soluble lysosomal aspartyl glycoprotease that can degrade the protein components of the matrix and free growth factors therein embedded, thus favoring tumor growth, invasion, and angiogenesis. The present work aimed to compare the expression of GP88 and Cath-D as novel prognostic biomarkers in human invasive ductal carcinoma (IDC) versus benign tumors and normal breast tissues as well as their correlation with different pathological and histological parameters. The immunohistochemical technique was used to examine the expression of GP88 and Cath-D in normal, benign, and IDC. Present results showed higher expression of GP88, and Cath-D in IDC compared to normal and benign breast tissues.
Progranulin 或 acrogranin 是一种 88 kDa 的糖蛋白,通过生物筛选确定了与高致瘤性相关的蛋白靶标。Cathepsin D(Cath-D)是一种可溶性溶酶体天冬氨酰糖蛋白酶,可降解基质中的蛋白质成分和嵌入其中的游离生长因子,从而有利于肿瘤的生长、侵袭和血管生成。本研究旨在比较作为新型预后生物标志物的 GP88 和 Cath-D 在人类浸润性导管癌(IDC)与良性肿瘤和正常乳腺组织中的表达情况,以及它们与不同病理和组织学参数的相关性。研究采用免疫组化技术检测了 GP88 和 Cath-D 在正常、良性和 IDC 组织中的表达。研究结果表明,与正常和良性乳腺组织相比,GP88和Cath-D在IDC中的表达量更高。
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引用次数: 0
Impact of Essential Oils (Orange Peels) on Ehrlich Ascites Carcinoma Against Cardiac Damage in Female Mice 精油(橘皮)对雌性小鼠艾氏腹水癌和心脏损伤的影响
Pub Date : 2024-03-02 DOI: 10.21608/jmals.2024.266129.1018
Ahmed Hasan, Haneen Hameed, Mohanad Hussein, A. Abbood, Assala Jawad, Marwa mahmoud Nada
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引用次数: 0
Giant bladder stones : Cases from a Late Consultation 巨型膀胱结石:晚期会诊病例
Pub Date : 2024-02-19 DOI: 10.21608/jmals.2024.264753.1017
Reda Tariqi, Abdelmounim Boughaleb, Hamza El Abidi, Adam El Aboudi, I. Boualaoui, Ahmed Ibrahimi, Hachem El Sayegh, Yassine Nouini
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引用次数: 0
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Journal of Medical and Life Science
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