Journal of Medical and Life Science最新文献

{"title":"Relationship between vitamin D3 levels and marital status: A random study in females suffering from bone disorders in Al-Najaf province","authors":"H. Khalfa, Hayder L. F. AL-Msaid, Rasha Alsahlanee","doi":"10.21608/jmals.2019.110859","DOIUrl":"https://doi.org/10.21608/jmals.2019.110859","url":null,"abstract":"Vitamin D is a fat-soluble steroid hormone. It obtained from foods or by the synthesis in the skin after sunlight exposure (Ultraviolet rays) and converted to the active form in the liver and kidneys. Vitamin D3 is highly affected by hormones of the menstrual cycle. Some evidence also suggests a possible role for vitamin D in the regulation of sexual function. There is no scientific evidence present that shows a relationship between marital status and vitamin D3 levels. The aim of this study is to investigate the correlation between vitamin D3 levels and marital status in females of different age groups in a given population. Methodology: A total of 140 females were randomly selected from the outpatient department in Al-Sader teaching hospital in AlNajaf province Iraq in the period of December 2018 and March 2019. Venous blood (5 ml) was drawn from all subjects. Subjects were classified according to their marital status (single, married) and age groups. Subjects taking vitamin D3 supplementation were discarded from the study. Vitamin D3 and calcium analysis were determined from Human serum using ELISA microtiter plate. Calcium levels mg/dl were also determined using serum (Sysmex XP-300 Atomic Hematology Analyzer). Results: The results of this study showed that all females of all age groups suffered from hypovitaminosis D3 due to bone disorders. The levels of vitamin D3 in all age groups single females were significantly (P<0.05) lower than that in married subjects. Serum calcium levels were statistically significant (P<0.05) lower in females that are single compared to married females. Conclusion: Females that were single had lower vitamin D3 levels as well as lower serum calcium levels compared to those married. The outcome of this study shows that married females have higher vitamin D3 levels compared to that of single subjects.","PeriodicalId":406966,"journal":{"name":"Journal of Medical and Life Science","volume":"37 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2019-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"130700073","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Dielectric spectroscopy of red blood cells in a randomized sample of anemic and non-anemic Egyptian first trimester pregnant and non-pregnant women in the rural district of Alexandria city: a primitive screening approach","authors":"M. Moustafa","doi":"10.21608/jmals.2019.68362","DOIUrl":"https://doi.org/10.21608/jmals.2019.68362","url":null,"abstract":"In Egypt, iron-deficiency anemia (I.D.A) is the most prevalent public health problem related to socioeconomics and lifestyle. Despite ferritin assay is the gold standard for (I.D.A), it is expensive and can be conflicted with other diseases. Hereby, new screening tools were emerged to monitor I.D.A. A sophisticated physical method depending on the electrical relaxation of R.B.Cs in anemic first trimester pregnant Egyptian women of a rural district in Alexandria was conducted. I.D.A was confirmed in all participant anemic women through calculated ferritin saturation percent. The dielectric permittivity and conductivity curves between control non-anemic non-pregnant and non-anemic pregnant women showed slight significant discrimination. These findings may be a primitive approach for I.D.A screening and further physical parameters will be needed to strengthen the idea.","PeriodicalId":406966,"journal":{"name":"Journal of Medical and Life Science","volume":"42 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2019-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"115806834","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Antimicrobial activity of Acmella caulirhiza on Candida albican and Escherichia coli","authors":"F. OdhiamboO., I. Abwao, M. GithaigaB., P. WaithakaN.","doi":"10.21608/jmals.2019.68363","DOIUrl":"https://doi.org/10.21608/jmals.2019.68363","url":null,"abstract":"Drug-resistant pathogens pose a big problem to the word today. Some infections resulting from drug-resistant pathogens do not respond to some of the drugs in the market today. This created a need for the development of drugs that are not resisted by the pathogens. This study was conceived with the aim of extracting antimicrobial compounds from Acmella caulirhiza. The Acmella caulirhiza plant was obtained from botanic garden in Egerton University. Plant extracts from leaves, stems, and flowers were obtained separately using methanol. The antimicrobial activity of the extracts against Candida albicans and Escherichia coli was carried out using Kirby Bauer’s disc diffusion technique. Minimum Inhibitory Concentration (MIC) was carried out using the microliter technique. The data obtained were analyzed using the Statistical Package of Social Sciences (SPSS) version 22 software. The means were separated using one-way analysis of variance (ANOVA). A P value < 0.05 was considered as significant. Acmella caulirhiza leaf, stem and flowers leaf extracts had antimicrobial properties against C. albicans and to E. coli. There is a need to further purify the antimicrobial metabolites from Acmella caulirhiza and determine the mechanisms for their action against E. coli and Candida albicans.","PeriodicalId":406966,"journal":{"name":"Journal of Medical and Life Science","volume":"39 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2019-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"127063346","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The validity of indirect haemagglutination assay (IHA) in the detection of Schistosoma haematobium infection relative to microscopic examination","authors":"A. Yameny","doi":"10.21608/jmals.2019.68364","DOIUrl":"https://doi.org/10.21608/jmals.2019.68364","url":null,"abstract":"Background : Several schistosome serodiagnostic assays have been developed over the last years, most of them are designed for the detection of anti-schistosomal antibodies which might remain for long periods of time after treatment, this study aimed to evaluate commercially available kit, indirect hemagglutination assay (IHA) as alternative to microscopic examination to diagnose S.haematobium infection Subjects and methods: This study is a cross-sectional study designed to evaluate an indirect hemagglutination assay (IHA), a commercially available kit was used for determine urinary schistosomiasis in the study population, this performed on 50 positive S.haematobium samples and 50 negative samples for individuals matching in age and gender, in addition, urine microscopic examination was done for detection of S.haematobium eggs by sedimentation centrifugation and nuclepore filtration techniques. Results:  The results showed that an indirect hemagglutination assay (IHA) had a sensitivity of 42% and specificity of 80%. PPVs were 67.7% whereas NPVs were 57.97 %. As for diagnostic efficiency, it was 61%, where the area under the curve (AUC) was bad 0.58. Conclusion and Recommendations: Years ago this commercial kit which used in this study and also used in all medical laboratories in Egypt for detection of schistosome antibody in serum is called Fumouzebilharzial IHA Kit, this study showed low sensitivity (42%) and specificity (80%), that lower than expected results for detection of antibody and the kit is generally more expensive than microscopic examination for S.haematobium infection.","PeriodicalId":406966,"journal":{"name":"Journal of Medical and Life Science","volume":"29 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2019-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"125183791","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Using Egyptian fruit bat (Rousettus aegyptiacus) as a bioindicator of environmental health, across some Egyptian Governorates","authors":"Basma M. Sheta, M. Beheary","doi":"10.21608/jmals.2019.68361","DOIUrl":"https://doi.org/10.21608/jmals.2019.68361","url":null,"abstract":"Bats are a good animal model of monitoring habitat pollution by heavy metal concentration assessment. A total of 50 Egyptian fruit bat were sampled from five governorates: Beni Suef, Damietta, Fayoum, Giza and Menofia. Fur samples were collected from these bats to be tested for the presence of heavy metals by using Atomic Absorption Spectrophotometry. Seven heavy metals (manganese (Mn), zinc (Zn), cadmium (Cd), chromium (Cr), Ferrum (Fe), nickel (Ni), and lead (Pb)) have been estimated. Statistical analysis with One-Way Analysis of Variance (ANOVA) revealed a significant difference of heavy metals concentration in the fur of bats at different studied governorates. No statistically differences in fur metals were observed between males and females fruit bat, except for Pb. No, correlation was observed between bat heaviness and fur heavy metals content. This study confirms bat fur as a biomarker for heavy metal contamination. The results showed that Beni Suef samples were the most contaminated with different heavy metals among the other governorates, followed by Damietta samples. Bats from Menofia and Fayoum governorates were the cleanest.","PeriodicalId":406966,"journal":{"name":"Journal of Medical and Life Science","volume":"1 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2019-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"123556094","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Biochemical Risk Factors for Kidney Stones in Diabetic Patients","authors":"Ahmed H. Hamzawy, A. Hussein, A. Ghobish, Z. Nabil","doi":"10.21608/jmals.2019.67991","DOIUrl":"https://doi.org/10.21608/jmals.2019.67991","url":null,"abstract":"Objective: The aim of this study is to investigate the biochemical risk factors of kidney stones in diabetes. This includes the estimation of serum and urinary creatinine (Cr), albumin (Alb), sodium (Na+), potassium (K+), magnesium (Mg++), phosphorous (Po4++), calcium (Ca++), uric Acid (U.A), alkaline phosphatase (ALP), serum parathyroid hormone (PTH), reduced glutathione levels(GSH), urinary Oxalate, as well as citrate and nitric oxide (NO) levels were measured. Subjects and Methods: The clinical study was performed on 100 patients that attended Suez Health Insurance Organization and Ain Elhyah Hospital. Patients were divided into four groups; 25 diabetic patients (D) (mean age 52); 25 renal stone patients (R, S) (mean age 49); 25 diabetic renal stone patients (D R S)(mean age 51) and 25 control subjects (C)((mean age 47) age-matched, randomly selected. Blood and urine 24 hr samples were collected. Results: Serum (Cr) and (Alb) levels showed a non-significant changes in all patients. However, the data showed a significant decrease in (Mg++) level of all patients groups, with a significant level in (D R S) than (D) and (R, S) patients. Moreover, a significant increase in (U.A) levels of all patients groups but higher in (DRS) than (D) and (R, S) patients groups, the data revealed a significant decrease in urinary magnesium level in(DRS) patients group compared to (C), (R, S) and (D) patients groups. Moreover, a significant increase in urinary (U.A) level of all patients was noticed, it was significantly higher level in (DRS) than (R, S) patients groups. Oxalate level in all patients was increased, significantly. Meanwhile, the oxalate level was increased in (DRS) compared to (R, S) and (D) patients groups. The citrate level was decreased significantly in the urine of all patients groups with a significantly lower level of (DRS) than (R, S) and (D) patients groups. On other hand, a significant decrease in blood GSH level of all patients groups was observed where; its level in (DRS) was significantly lower than the (R, S) patients groups. However, data revealed a significant increase in serum (PTH) level of the (R, S) and (D R S) patients groups compared to (C) one. PTH level in (R, S) patients group was significantly higher than (D) and (DRS) patients groups. The data revealed a significant increase in serum ALP level of (D) and (D R S) groups compared to (C) group with a higher significant level in (DRS) than (R,S) patients groups. Moreover, the urinary 24h (NO) level was increased significantly in all patients groups compared to (C) group.","PeriodicalId":406966,"journal":{"name":"Journal of Medical and Life Science","volume":"251 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2019-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"123541483","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Assessment of Serum Urea, Creatinine, and electrolytes in Patients with Prostate Cancer under Androgen Deprivation Therapy","authors":"S. Elhassan, Y. Suliman, G. Modawe","doi":"10.21608/jmals.2019.68036","DOIUrl":"https://doi.org/10.21608/jmals.2019.68036","url":null,"abstract":"Background: Prostate cancer is the most common cancer in a male of the reproductive system and associated with high mortality and Androgen-deprivation therapy (ADT) is a key component of treatment for aggressive and advanced prostate cancer. Objective: The aim of this study was to assess the serum urea and creatinine beside the measure of serum electrolytes (Na and K) in patients with prostate cancer under (ADT).Methodology: 100 Sudanese patients with prostate cancer (PCa) under (ADT) attended in Khartoum oncology hospital, Khartoum state. The study conducted in April and July 2018. The age of PCa patients ranged between (50 to73 years) and their mean 62.2 years. The blood samples were taken from each patient. The urea and creatinine were estimated using spectrophotometer methods and also the serum electrolytes were measured by using flame photometers methods. Results: The (mean±SD) of serum urea, creatinine, Na and K were (41.31±27.6mg/dl,1.26±0.93mg/dl,141.37±11.52mg/dl,4.38±3.89mg/dl) respectively. The concentration of serum urea, creatinine, Na and K were normal within a normal range. There was significantly (P=0.000) difference of serum urea among prostate cancer patients under ADT and there was a significant correlation between urea and creatinine and also between Na and K. Conclusion: In this study concluded that the ADT  has no effect on blood renal parameters among prostate cancer.","PeriodicalId":406966,"journal":{"name":"Journal of Medical and Life Science","volume":"18 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2019-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"123664341","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Amelioration of the therapeutic efficacy of 5-Flurouracil loaded chitosan nanoparticles in experimentally induced Hepatocellular Carcinoma","authors":"El-Hassan M. Mokhamer, M. Yehia, H. Ramadan, Ola EL-Gendy","doi":"10.21608/jmals.2019.67843","DOIUrl":"https://doi.org/10.21608/jmals.2019.67843","url":null,"abstract":"Hepatocellular carcinoma (HCC) is the most common type of liver cancer. The most important risk factor for the development of HCC is cirrhosis regardless of etiology. 5-Fluorouracil (5-FU) is widely used in the treatment of cancer. Drug resistance remains a significant limitation to the clinical use of 5-FU. The present study aimed to evaluate the therapeutic efficacy of 5-FU loaded chitosan nanoparticles in experimentally induced HCC. To achieve our purpose, one hundred and five male Swiss Albino mice were divided randomly into two major groups:  Group A: comprised 25 mice served as normal control, Group B: comprised 80 mice received a daily oral dose of 0.06% DAB (165 mg/kg body.wt.) for 30 days after which the water was replaced with 0.05% aqueous solution of Phenobarbital (PB). Five chosen mice randomly from groups A and B at the time intervals 15, 30, 45 and 60 days were sacrificed to follow up with the development of HCC by biochemical and histopathological examination. Animals of group B were divided into 3 groups Group I: included 20 mice served as an untreated group, group II: included 20 mice injected intraperitoneally with 5-FU only (40mg/kg body.wt) every 2 days for 16 days, group III:  included 20 mice injected intraperitoneally with 5-FU Cs NPs. Each group was further divided into two subgroups 10 mice each, one subgroup treated with ultrasonic waves; meanwhile the other subgroup without ultrasonic waves exposure. At the end of the experiment, animals were sacrified, serum ALT, hepatic ALT, and hepatic MDA were estimated; HCC was histopathologically monitored in all studied groups. There was 276.5%, 145.7%and 438.5% increase in serum ALT, hepatic ALT and hepatic MDA levels respectively comparing to the corresponding control. Liver tumors that ultimately became neoplastic were produced after 45 days. US exposure triggered a significant decline in serum and hepatic ALT activity (P = 0.001) and in hepatic MDA (P = 0.009) within 5-FU loaded Cs NPs group. Moreover, tumor growth delay and more enhanced correction in hepatic architecture was obtained by a combination of US and 5-FU loaded Cs NPs therapy. Based on these results, we can conclude that the use of 5-FU loaded chitosan nanoparticles in combination with low-intensity ultrasound ameliorates the efficacy of 5-FU as anticancer therapy for HCC.","PeriodicalId":406966,"journal":{"name":"Journal of Medical and Life Science","volume":"42 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2019-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"124764740","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Anti-proliferative effect of ZnO NPs against the growth of Ehrlich Solid carcinoma","authors":"L. Dawood, E. Tousson, Afaf M. El-Atrsh, A. Salama","doi":"10.21608/jmals.2019.180380","DOIUrl":"https://doi.org/10.21608/jmals.2019.180380","url":null,"abstract":"Background: Zinc oxide nanoparticle (ZnO NPs) has been widely used in biomedical applications and cancer therapy and has been reported to induce a selective cytotoxic effect on cancer cell proliferation. Methods: Female Swiss Albino mice were inoculated subcutaneously with Ehrlich ascites carcinoma (EAC) cells. ZnO NPs or MTX were injected intraperitoneally. Tumor growth inhibition rate (TIR %), increase in life span (ILS%), zinc concentration, lipid peroxidation marker (MDA), glutathione (GSH) contents, and the activity of the antioxidant scavenger enzymes SOD and catalase CAT was examined. Also, DNA fragmentation and histopathological studies of all groups were carried out. Results: Treatment of tumor-bearing mice with ZnO NPs significantly increased (MST), (TIR %), (ILS %) and reduced tumor weight, compared to tumor-bearing controls. Additionally, Zn concentration and DNA fragmentation were significantly increased in tumor tissues. ZnO nanoparticles were also found to induce oxidative stress, evidenced by the generation of reactive oxygen species and depletion of the antioxidants in tumor tissues, with no changes in the liver. Our data demonstrate that ZnO NPs exert distinct effects via the killing of cancer cells. Conclusion: The study findings demonstrated that the ZnO NPs can induce selective cytotoxicity through reactive oxygen species generation and oxidative stress.","PeriodicalId":406966,"journal":{"name":"Journal of Medical and Life Science","volume":"50 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"1900-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"121401987","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Relationship between interleukin 17 & 6 in patients with varicocele compare with a control group","authors":"Hayder L. F. AL-Msaid, W. H., H. Khalfa","doi":"10.21608/jmals.2019.110862","DOIUrl":"https://doi.org/10.21608/jmals.2019.110862","url":null,"abstract":"The spermatogenesis process is very complex and involves many processes that lead to giving normal sperm, these sperms are very important for Fertility in the male. The cytokines are very important in male fertility which gives a significant job in fertilization. The varicocele patient grade 1,2 and 3(n= 25 , 25 and 25 Respectively) compared with control patient (n=13). The results of this study showed that cytokine level (interleukin 17 & 6) is a significant increase in varicocele patient compare with the control group, also a positive correlation was found between interleukin 17 & interleukin 6 in varicocele patient, while the result showed a negative correlation between interleukin 17 and the sperms concentration, sperm Progressive motility percent and sperm normal morphology present in a patient with varicocele grade 1,2 and 3 respectively (r= -0. 574, r = -0. 647and r = -0. 487). Also a negative correlation between interleukin 6 level and the sperms concentration , sperm Progressive motility percent and sperm normal morphology present in varicocele patient grade 1,2 and 3 Respectively (r= -0.467 , r = -0.324 and r = -0.307 ). The present study concludes that important role of interleukin 17 and interleukin 6 appears to be a key cytokine regulating local tissue inflammation, the study showed varicocele has a Harmful effect with the time on cytokines levels and increases the diameter.","PeriodicalId":406966,"journal":{"name":"Journal of Medical and Life Science","volume":"101 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"1900-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"133373082","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}