Pub Date : 2022-06-01DOI: 10.21608/jmals.2022.245127
Farouk Hachem, H. Abdallah, A. Ibrahimi, H. Elsayegh, Y. Nouini
The sarcomatoid variant of urothelial carcinoma (SVUC) is an uncommon histological variant of urothelial carcinoma. Sarcomatoid carcinomas are aggressive malignancies with poor prognosis, which by definition are biphasic neoplasms with both an epithelial and a mesenchymal component. There is no consensus opinion on the best treatment modalities for this tumor. Herein on are reported 5 cases of SVUC with a brief review of the literature. The data was collected from the medical records in the Department of Urology, at the University Hospital Center of Rabat Morocco during the period from January 2014 to December 2017.
{"title":"Five Case Reports of Sarcomatoid Variant of Urothelial Carcinoma of the Bladder","authors":"Farouk Hachem, H. Abdallah, A. Ibrahimi, H. Elsayegh, Y. Nouini","doi":"10.21608/jmals.2022.245127","DOIUrl":"https://doi.org/10.21608/jmals.2022.245127","url":null,"abstract":"The sarcomatoid variant of urothelial carcinoma (SVUC) is an uncommon histological variant of urothelial carcinoma. Sarcomatoid carcinomas are aggressive malignancies with poor prognosis, which by definition are biphasic neoplasms with both an epithelial and a mesenchymal component. There is no consensus opinion on the best treatment modalities for this tumor. Herein on are reported 5 cases of SVUC with a brief review of the literature. The data was collected from the medical records in the Department of Urology, at the University Hospital Center of Rabat Morocco during the period from January 2014 to December 2017.","PeriodicalId":406966,"journal":{"name":"Journal of Medical and Life Science","volume":"62 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"123461534","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-03-01DOI: 10.21608/jmals.2022.229304
Alaa Ali, G. Modawe, M. Rida, A. Abdrabo
: Background: More than 70 million couples worldwide are affected by infertility. With a male factor infertility account for about half of the cases. Semen analysis is critical for determining male fertility potential. Objective : To estimate the prevalence of abnormal semen parameters among Sudanese Patients Fertility Center in Khartoum city, Sudan. Materials and methods: This is a retrospective cross-sectional study conducted on 2585 males who were referred to the fertility Center in Khartoum, in 2018, to evaluate the semen quality (sperm count, motility, and morphology) according to World Health Organization (WHO) criteria. The descriptive statistics and frequency studies were conducted using the SPSS version (23). Results: There is a higher prevalence of abnormal semen parameters 86.8 % (sperm count 14.9% - sperm motility 0.7% - morphology 40.7% and 30.5% is a combination of two or more abnormal sperm counts). Sperm motility and morphology and only 13.2% showed normal semen parameters according to WHO criteria. Conclusion: the finding of this study revealed there is low semen quality. It shows a high prevalence of low sperm count, abnormal motility, and morphology according to the WHO criteria.
{"title":"Prevalence of Abnormal Semen Parameters among Male Patients Attending the Fertility Center in Khartoum, Sudan","authors":"Alaa Ali, G. Modawe, M. Rida, A. Abdrabo","doi":"10.21608/jmals.2022.229304","DOIUrl":"https://doi.org/10.21608/jmals.2022.229304","url":null,"abstract":": Background: More than 70 million couples worldwide are affected by infertility. With a male factor infertility account for about half of the cases. Semen analysis is critical for determining male fertility potential. Objective : To estimate the prevalence of abnormal semen parameters among Sudanese Patients Fertility Center in Khartoum city, Sudan. Materials and methods: This is a retrospective cross-sectional study conducted on 2585 males who were referred to the fertility Center in Khartoum, in 2018, to evaluate the semen quality (sperm count, motility, and morphology) according to World Health Organization (WHO) criteria. The descriptive statistics and frequency studies were conducted using the SPSS version (23). Results: There is a higher prevalence of abnormal semen parameters 86.8 % (sperm count 14.9% - sperm motility 0.7% - morphology 40.7% and 30.5% is a combination of two or more abnormal sperm counts). Sperm motility and morphology and only 13.2% showed normal semen parameters according to WHO criteria. Conclusion: the finding of this study revealed there is low semen quality. It shows a high prevalence of low sperm count, abnormal motility, and morphology according to the WHO criteria.","PeriodicalId":406966,"journal":{"name":"Journal of Medical and Life Science","volume":"44 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"125513018","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-03-01DOI: 10.21608/jmals.2022.230513
A. Soliman, Basma M. Sheta, Mohamed Bahnasway, G. Orabi
: St. Catherine is a unique and remarkable stopover place for migratory birds and a high-quality resource habitat for resident birds. St. Catherine is an important bird area (IBA) and declared a protectorate. In the present study, the bird community was surveyed across a gradient of different habitat types across the St. Catherine protectorate. A total of 3884 individuals belonging to 73 bird species (represented by 12 orders and 28 families) were recorded during the period from June 2017 to August 2018. The total resident bird species richness was 25 bird species with 3100 individuals in abundance belonging to 7 orders and 16 families. The most abundant resident bird species were Chukar partridge ( Alectoris chukar ), Rock Dove ( Columba livia ), Sinai Rose Finsh ( Carpodacus synoicus ), and Desert Lark ( Ammomanes deserti ). The crossing over Palearctic migratory species was higher than resident species, with a richness of 48 bird species related to 8 orders and 18 families. The top abundant migrants were White Pelican ( Pelecanus onocrotalus ), lesser whitethroat ( Sylvia curruca ), Chiffchaff ( Phylloscopus collybita ), and European honey buzzard ( Pernis apivorus ). This survey showed the importance of St. Katherine for both resident and migratory birds. A continuous standardized bird survey is required in this very special protectorate for both migratory and resident bird species.
{"title":"Avifaunal updated survey in St. Catherine protectorate, Egypt","authors":"A. Soliman, Basma M. Sheta, Mohamed Bahnasway, G. Orabi","doi":"10.21608/jmals.2022.230513","DOIUrl":"https://doi.org/10.21608/jmals.2022.230513","url":null,"abstract":": St. Catherine is a unique and remarkable stopover place for migratory birds and a high-quality resource habitat for resident birds. St. Catherine is an important bird area (IBA) and declared a protectorate. In the present study, the bird community was surveyed across a gradient of different habitat types across the St. Catherine protectorate. A total of 3884 individuals belonging to 73 bird species (represented by 12 orders and 28 families) were recorded during the period from June 2017 to August 2018. The total resident bird species richness was 25 bird species with 3100 individuals in abundance belonging to 7 orders and 16 families. The most abundant resident bird species were Chukar partridge ( Alectoris chukar ), Rock Dove ( Columba livia ), Sinai Rose Finsh ( Carpodacus synoicus ), and Desert Lark ( Ammomanes deserti ). The crossing over Palearctic migratory species was higher than resident species, with a richness of 48 bird species related to 8 orders and 18 families. The top abundant migrants were White Pelican ( Pelecanus onocrotalus ), lesser whitethroat ( Sylvia curruca ), Chiffchaff ( Phylloscopus collybita ), and European honey buzzard ( Pernis apivorus ). This survey showed the importance of St. Katherine for both resident and migratory birds. A continuous standardized bird survey is required in this very special protectorate for both migratory and resident bird species.","PeriodicalId":406966,"journal":{"name":"Journal of Medical and Life Science","volume":"194 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"121061330","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-12-24DOI: 10.21608/jmals.2021.210630
R. Abdel-Aal, O. Hussein, Reham G. Elsaady, Lobna Abdelzaher
Neuroinflammation plays a crucial role in Alzheimer's disease (AD) pathogenesis. Apoptosis, along with impaired neurogenesis, has been linked to AD neurodegenerative cell death, likely due to overexpression of cyclooxygenase2 (COX-2). We investigated whether the concurrent administration of celecoxib, a selective COX-2 inhibitor, with rivastigmine, the standard anti-Alzheimer, would enhance rivastigmine anti-Alzheimer activity in the aluminum chloride (AlCl3) Alzheimer's rat model. Male rats were randomly assembled into control (Cont), AlCl3-treated (Al), rivastigmine-treated (RIVA), celecoxib-treated (Celeco), and combined rivastigmine and celecoxib-treated (RIVA+Celeco) groups. They were studied for memory, and cognitive skills, along with evaluating hippocampal acetylcholinesterase (AChE) activity. Hippocampal neuropathology, besides apoptosis, astroglial injury, and neurogenesis, were assessed through examining the expression of their related protein markers; activated caspase-3, glial fibrillary acidic protein (GFAP), and nestin. Celecoxib, rivastigmine, and their combination attenuated AlCl3induced intellectual impairment and the associated neurodegenerative changes. However, the combination therapy had no additional neuroprotective advantage over rivastigmine alone, except for the enhancement of neurogenesis and suppression of apoptosis in the AL-intoxicated rats. As compared to rivastigmine, the efficacy of celecoxib in combination with rivastigmine confers neuroprotection only at the cellular level, enhancement of neurogenesis, and suppression of apoptosis, without having a mitigating effect on Al-induced cognitive impairment.
{"title":"Celecoxib effect on rivastigmine anti-Alzheimer activity against aluminum chloride-induced neurobehavioral deficits as a rat model of Alzheimer's disease; novel perspectives for an old drug","authors":"R. Abdel-Aal, O. Hussein, Reham G. Elsaady, Lobna Abdelzaher","doi":"10.21608/jmals.2021.210630","DOIUrl":"https://doi.org/10.21608/jmals.2021.210630","url":null,"abstract":"Neuroinflammation plays a crucial role in Alzheimer's disease (AD) pathogenesis. Apoptosis, along with impaired neurogenesis, has been linked to AD neurodegenerative cell death, likely due to overexpression of cyclooxygenase2 (COX-2). We investigated whether the concurrent administration of celecoxib, a selective COX-2 inhibitor, with rivastigmine, the standard anti-Alzheimer, would enhance rivastigmine anti-Alzheimer activity in the aluminum chloride (AlCl3) Alzheimer's rat model. Male rats were randomly assembled into control (Cont), AlCl3-treated (Al), rivastigmine-treated (RIVA), celecoxib-treated (Celeco), and combined rivastigmine and celecoxib-treated (RIVA+Celeco) groups. They were studied for memory, and cognitive skills, along with evaluating hippocampal acetylcholinesterase (AChE) activity. Hippocampal neuropathology, besides apoptosis, astroglial injury, and neurogenesis, were assessed through examining the expression of their related protein markers; activated caspase-3, glial fibrillary acidic protein (GFAP), and nestin. Celecoxib, rivastigmine, and their combination attenuated AlCl3induced intellectual impairment and the associated neurodegenerative changes. However, the combination therapy had no additional neuroprotective advantage over rivastigmine alone, except for the enhancement of neurogenesis and suppression of apoptosis in the AL-intoxicated rats. As compared to rivastigmine, the efficacy of celecoxib in combination with rivastigmine confers neuroprotection only at the cellular level, enhancement of neurogenesis, and suppression of apoptosis, without having a mitigating effect on Al-induced cognitive impairment.","PeriodicalId":406966,"journal":{"name":"Journal of Medical and Life Science","volume":"1 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2021-12-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"122970637","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-12-24DOI: 10.21608/jmals.2021.311230
H.A.S. Elelimy
Spilostethus pandurus is a phytophagous bug, which is widely distributed in tropical and subtropical areas. The reproductive system of the males of Spilostethus pandurus includes two testes, two seminal vesicles, two vasa deferentia, and one pair of accessory glands. The male accessory glands of Spilostethus pandurus are termed mesadenia and open into the vas deferens. The accessory glands of males (mesadenia) take an irregular shape like a sack (with multiple lobes). The mesadenia accessory glands comprise one mono-epithelial layer of secretory cells lying on a thin basal lamina and encompassed in a muscular layer. This research describes the structure and ultrastructure of the accessory glands of males using scanning and transmission electron microscopy.
{"title":"Morphology and ultrastructure of the male accessory gland of Spilostethus pandurus (Scopoli) (Hemiptera: Lygaeidae)","authors":"H.A.S. Elelimy","doi":"10.21608/jmals.2021.311230","DOIUrl":"https://doi.org/10.21608/jmals.2021.311230","url":null,"abstract":"Spilostethus pandurus is a phytophagous bug, which is widely distributed in tropical and subtropical areas. The reproductive system of the males of Spilostethus pandurus includes two testes, two seminal vesicles, two vasa deferentia, and one pair of accessory glands. The male accessory glands of Spilostethus pandurus are termed mesadenia and open into the vas deferens. The accessory glands of males (mesadenia) take an irregular shape like a sack (with multiple lobes). The mesadenia accessory glands comprise one mono-epithelial layer of secretory cells lying on a thin basal lamina and encompassed in a muscular layer. This research describes the structure and ultrastructure of the accessory glands of males using scanning and transmission electron microscopy.","PeriodicalId":406966,"journal":{"name":"Journal of Medical and Life Science","volume":"32 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2021-12-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"123729089","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-06-27DOI: 10.21608/jmals.2021.240126
S. Alabd, A. Yameny
Background: the identification of a simple and effective prognosticator is crucial for high lightening and treating the potentially critical patients, to reduce the mortality rate, Serum CRP is a simple and effective prognosticator which casts light on potentially critical patients, Serum CRP has a predictive value of COVID19. Patients and methods: This study included 328 out-hospitalized patients with confirmed COVID-19 infection, these study subjects were randomly selected irrespective of the age group and both genders were included, Assay procedure as manufactory instructions and Reagents of Cromatest Linear chemicals S.L.U. Barcelona Spain turbidimetric method is used. Results: Female gender was more frequent (n=192, 58.5%) than Male gender (n=136, 41.5%). This study reveals a high level of CRP in 296 patients (90.2%) with a sensitivity of 90.2%, which is divided into two groups the first have CRP levels 5-100 (56.1%) and the second group has CRP levels more than 100 (34.1%), the high-level CRP was a significant biomarker for COVID-19 diagnosis or prognosis in out-hospitalized patients (Outpatients and patients under home observation), with a p-value is p is < .00001which less than 0.05. Conclusion: CRP levels were positively correlated with mild COVID-19 infection of lung lesions with a sensitivity of 90.2%. CRP levels could reflect disease severity and should be used as a key indicator for disease monitoring.
{"title":"C-Reactive Protein as a Prognostic Indicator in COVID-19 mild infection Patients","authors":"S. Alabd, A. Yameny","doi":"10.21608/jmals.2021.240126","DOIUrl":"https://doi.org/10.21608/jmals.2021.240126","url":null,"abstract":"Background: the identification of a simple and effective prognosticator is crucial for high lightening and treating the potentially critical patients, to reduce the mortality rate, Serum CRP is a simple and effective prognosticator which casts light on potentially critical patients, Serum CRP has a predictive value of COVID19. Patients and methods: This study included 328 out-hospitalized patients with confirmed COVID-19 infection, these study subjects were randomly selected irrespective of the age group and both genders were included, Assay procedure as manufactory instructions and Reagents of Cromatest Linear chemicals S.L.U. Barcelona Spain turbidimetric method is used. Results: Female gender was more frequent (n=192, 58.5%) than Male gender (n=136, 41.5%). This study reveals a high level of CRP in 296 patients (90.2%) with a sensitivity of 90.2%, which is divided into two groups the first have CRP levels 5-100 (56.1%) and the second group has CRP levels more than 100 (34.1%), the high-level CRP was a significant biomarker for COVID-19 diagnosis or prognosis in out-hospitalized patients (Outpatients and patients under home observation), with a p-value is p is < .00001which less than 0.05. Conclusion: CRP levels were positively correlated with mild COVID-19 infection of lung lesions with a sensitivity of 90.2%. CRP levels could reflect disease severity and should be used as a key indicator for disease monitoring.","PeriodicalId":406966,"journal":{"name":"Journal of Medical and Life Science","volume":"11 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2021-06-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"128316862","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-06-23DOI: 10.21608/jmals.2021.179658
Mohammed Y. Basher, Asia M. Elrashied, S. Elbager, S. Khalil
Introduction: The PIK3R1 (phosphoinositide-3-kinase regulatory subunit 1) and PlGF (Placenta growth factor)genes share some common pathways with eNOS,( Endothelial nitric oxide synthase) that plays a vital role in angiogenesis of blood vessels and regulating endothelial function. The PIK3R1 gene encodes the receptor for the p85α regulatory subunit of the phosphoinositide-3-kinase (PI3K), that involved in endothelial cell migration. The PlGF gene encodes for the placenta growth factor, a homolog of vascular endothelial growth factor (VEGFA) that is involved in angiogenesis. PIK3R1 and/or PlGF mutations may cause dysregulation of eNOS contributing to endothelial dysfunction in preeclampsia. This study aims to analyze the effect of mutation of the PIK3R1 and PlGF genes on the structure and function of PIK3R1 and PGF protein that may have an important role in pathogeneses of preeclampsia. Methodology: The data on human PIK3R1and PGF genes were retrieved from dbSNP/NCBI. Ten prediction algorithms; SIFT, PROVEAN, Polyphen, SNAP2, SNPs&GO, PANTHER PhD-SNP, I-Mutant, Mutpred, and Hope were used to analyzing the effect of nsSNPs on functions and structure of the PGF and PIK3R1 protein. STRING and KEGG databases were used for PGF and PIK3R1 protein-protein interaction. Results and Discussion: As per the dbSNP database, the humanPIK3R1gene contained 365 missense mutations. A total 3nsSNPs (T239M, S229W, E47K) and 2 nsSNPs (H125Y, V59G) were predicted to have the most damaging effects on the structure and function of PIK3R1 and PGF respectively. STRING and KEGG revealed that PIK3R1and PGF had strong interactions with proteins involved in the VEGF signaling pathway and PI3K-Akt signaling pathway. PIK3R1 is confirmed to be linked to important diseases like preeclampsia and can affect its treatment response. Conclusion: Gene-gene interaction is an important factor in preeclampsia treatment, effect of mutation of the PIK3R1 and PlGF genes on the structure and function of PIK3R1 and PGF protein may have an important role in pathogeneses of preeclampsia. Also, these are linked to its treatment effect. This document gives formatting instructions for authors preparing papers for publication in the journal. Authors are encouraged to prepare manuscripts directly using this template. This template demonstrates the format requirements for the Journal.
{"title":"In silico study of gene-gene interaction of PIK3R1and PIGF deleterious SNPs in correlation to the preeclampsia risk and its haplotype effect on antihypertensive treatment","authors":"Mohammed Y. Basher, Asia M. Elrashied, S. Elbager, S. Khalil","doi":"10.21608/jmals.2021.179658","DOIUrl":"https://doi.org/10.21608/jmals.2021.179658","url":null,"abstract":"Introduction: The PIK3R1 (phosphoinositide-3-kinase regulatory subunit 1) and PlGF (Placenta growth factor)genes share some common pathways with eNOS,( Endothelial nitric oxide synthase) that plays a vital role in angiogenesis of blood vessels and regulating endothelial function. The PIK3R1 gene encodes the receptor for the p85α regulatory subunit of the phosphoinositide-3-kinase (PI3K), that involved in endothelial cell migration. The PlGF gene encodes for the placenta growth factor, a homolog of vascular endothelial growth factor (VEGFA) that is involved in angiogenesis. PIK3R1 and/or PlGF mutations may cause dysregulation of eNOS contributing to endothelial dysfunction in preeclampsia. This study aims to analyze the effect of mutation of the PIK3R1 and PlGF genes on the structure and function of PIK3R1 and PGF protein that may have an important role in pathogeneses of preeclampsia. Methodology: The data on human PIK3R1and PGF genes were retrieved from dbSNP/NCBI. Ten prediction algorithms; SIFT, PROVEAN, Polyphen, SNAP2, SNPs&GO, PANTHER PhD-SNP, I-Mutant, Mutpred, and Hope were used to analyzing the effect of nsSNPs on functions and structure of the PGF and PIK3R1 protein. STRING and KEGG databases were used for PGF and PIK3R1 protein-protein interaction. Results and Discussion: As per the dbSNP database, the humanPIK3R1gene contained 365 missense mutations. A total 3nsSNPs (T239M, S229W, E47K) and 2 nsSNPs (H125Y, V59G) were predicted to have the most damaging effects on the structure and function of PIK3R1 and PGF respectively. STRING and KEGG revealed that PIK3R1and PGF had strong interactions with proteins involved in the VEGF signaling pathway and PI3K-Akt signaling pathway. PIK3R1 is confirmed to be linked to important diseases like preeclampsia and can affect its treatment response. Conclusion: Gene-gene interaction is an important factor in preeclampsia treatment, effect of mutation of the PIK3R1 and PlGF genes on the structure and function of PIK3R1 and PGF protein may have an important role in pathogeneses of preeclampsia. Also, these are linked to its treatment effect. This document gives formatting instructions for authors preparing papers for publication in the journal. Authors are encouraged to prepare manuscripts directly using this template. This template demonstrates the format requirements for the Journal.","PeriodicalId":406966,"journal":{"name":"Journal of Medical and Life Science","volume":"17 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2021-06-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"122754616","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-03-31DOI: 10.21608/jmals.2021.200216
A. Yameny
Background: The D-dimer is formed by the activation of the plasmin enzyme, and elevated levels indicate that there is a hypercoagulable state and secondary fibrinolysis in the body, which is extremely useful for the diagnosis of thrombotic diseases. Patients with COVID-19 were reported to have a hypercoagulable state. Patients and methods: This study included 231 out hospitalized patients with confirmed COVID-19 infection in Alexandria, Egypt, these study subjects were randomly selected irrespective of the age group and both genders were included. Results: The present study included patients aged from14 years to 75 years mean age was 44.5 ±30.5 who were confirmed to have Covid-19 based on real-time reverse transcription-polymerase chain reaction, This study reveals high plasma D-dimer levels in 84 patients (36.4%), which was a significant biomarker for COVID-19 diagnosis in out-hospitalized patients (Outpatients and patients under home observation), with a p-value is 0.00001 which less than 0.05. Conclusion and Recommendations: False data about D-dimer was caused more deaths in Egypt for patients and physicians, they thought Anticoagulation therapy was only for hospitalized patients and not for out -hospitalized patients with moderate infection, they thought the D-dimer test is not important for outhospitalized patients, but this study showed a high level of D-dimer 36.4% in outhospitalized patients with COVID-19, so 36.4% of outpatients and patients under home observation need Anticoagulation therapy to decrease the complications of COVID-19 infection, Many individuals with diabetes and immunosuppressive diseases it is known that they face a higher probability to experience serious complications from COVID-19 infection so Anticoagulation therapy is suggested before the elevation of D-dimer level to avoid serious complications.
{"title":"D-dimer levels in COVID-19 out-hospitalized patients in Egypt","authors":"A. Yameny","doi":"10.21608/jmals.2021.200216","DOIUrl":"https://doi.org/10.21608/jmals.2021.200216","url":null,"abstract":"Background: The D-dimer is formed by the activation of the plasmin enzyme, and elevated levels indicate that there is a hypercoagulable state and secondary fibrinolysis in the body, which is extremely useful for the diagnosis of thrombotic diseases. Patients with COVID-19 were reported to have a hypercoagulable state. Patients and methods: This study included 231 out hospitalized patients with confirmed COVID-19 infection in Alexandria, Egypt, these study subjects were randomly selected irrespective of the age group and both genders were included. Results: The present study included patients aged from14 years to 75 years mean age was 44.5 ±30.5 who were confirmed to have Covid-19 based on real-time reverse transcription-polymerase chain reaction, This study reveals high plasma D-dimer levels in 84 patients (36.4%), which was a significant biomarker for COVID-19 diagnosis in out-hospitalized patients (Outpatients and patients under home observation), with a p-value is 0.00001 which less than 0.05. Conclusion and Recommendations: False data about D-dimer was caused more deaths in Egypt for patients and physicians, they thought Anticoagulation therapy was only for hospitalized patients and not for out -hospitalized patients with moderate infection, they thought the D-dimer test is not important for outhospitalized patients, but this study showed a high level of D-dimer 36.4% in outhospitalized patients with COVID-19, so 36.4% of outpatients and patients under home observation need Anticoagulation therapy to decrease the complications of COVID-19 infection, Many individuals with diabetes and immunosuppressive diseases it is known that they face a higher probability to experience serious complications from COVID-19 infection so Anticoagulation therapy is suggested before the elevation of D-dimer level to avoid serious complications.","PeriodicalId":406966,"journal":{"name":"Journal of Medical and Life Science","volume":"106 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2021-03-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"122470058","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2020-12-20DOI: 10.21608/jmals.2020.306876
A. E. El-Beltagy, Mohamed S. Elattar, Karoline K Abdelaziz, A. Attallah
Background: Mercury (Hg) is a toxic heavy metal that has been recognized as one of the strongest environmental pollutants. Exposure to Hg even in low doses can induce adverse effects on the structure and functions of the different body organs including gonads. Celery ( Apium graveolens ) leaves are rich with antioxidant compounds like apiin, apigenin, and quercetin, and vitamins like E and C. Aim: This work aimed to evaluate the possible modulatory role of celery leaves extract against gonadal toxicity induced by HgCl 2 in female rats and their pups during gestation and lactation periods. Material &methods: Twenty-four female Wistar rats (180-200g) were used in this experiment. They were mated with males. After confirmation of the gestation test, the pregnant rats were divided into four groups (six for each) as follows: control, celery extract (CE) supplement (200 mg/kg b.wt), HgCl 2 (2mg/kg b.wt) and HgCl 2 co-supplemented with CE groups. The experiment extended from the 4 th day of gestation till the end of the weaning period. At the end of weaning, the female rats and their pups were weighed and sacrificed to collect the blood and remove the gonads for estimation of biochemical and histopathological changes. Results: The obtained results revealed that exposure of female rats to HgCl 2 resulted in a significant decrease in the activities of antioxidant enzymes (SOD, CAT, and GPx) and sexual hormones (FSH, LH, estrogen, progesterone, and testosterone) in both mother rats and their pups if compared with control. Further, a light microscopic investigation revealed that exposure to HgCl2 during gestation and lactation periods induced deleterious histopathological changes in the ovaries of mothers as well as in the ovaries and testes of their offspring. Additionally, the ovarian sections from mothers and their offspring displayed weak to negative expression for calretinin antibody, however, the testicular sections from offspring appeared strongly stained with COX-2 antibody (inflammatory marker) and weakly stained with Ki67 antibody (proliferative marker). Further study by flow cytometric technique revealed a highly significant increase in the mean % value of apoptotic cells in both ovaries and testes. On the other hand, co-supplementation of CE to HgCl 2 treated group successfully alleviated the altered antioxidants and hormones as well as the histological changes near to the normal as control. Conclusion : Celery extract has a powerful ameliorative role against HgCl 2 -induced deleterious biochemical and histopathological changes in the gonads of female rats and their pups.
{"title":"Modulatory role of celery (Apium graveolens) leaves extract against mercuric chloride-induced gonadal dysfunction in female rats and their pups","authors":"A. E. El-Beltagy, Mohamed S. Elattar, Karoline K Abdelaziz, A. Attallah","doi":"10.21608/jmals.2020.306876","DOIUrl":"https://doi.org/10.21608/jmals.2020.306876","url":null,"abstract":"Background: Mercury (Hg) is a toxic heavy metal that has been recognized as one of the strongest environmental pollutants. Exposure to Hg even in low doses can induce adverse effects on the structure and functions of the different body organs including gonads. Celery ( Apium graveolens ) leaves are rich with antioxidant compounds like apiin, apigenin, and quercetin, and vitamins like E and C. Aim: This work aimed to evaluate the possible modulatory role of celery leaves extract against gonadal toxicity induced by HgCl 2 in female rats and their pups during gestation and lactation periods. Material &methods: Twenty-four female Wistar rats (180-200g) were used in this experiment. They were mated with males. After confirmation of the gestation test, the pregnant rats were divided into four groups (six for each) as follows: control, celery extract (CE) supplement (200 mg/kg b.wt), HgCl 2 (2mg/kg b.wt) and HgCl 2 co-supplemented with CE groups. The experiment extended from the 4 th day of gestation till the end of the weaning period. At the end of weaning, the female rats and their pups were weighed and sacrificed to collect the blood and remove the gonads for estimation of biochemical and histopathological changes. Results: The obtained results revealed that exposure of female rats to HgCl 2 resulted in a significant decrease in the activities of antioxidant enzymes (SOD, CAT, and GPx) and sexual hormones (FSH, LH, estrogen, progesterone, and testosterone) in both mother rats and their pups if compared with control. Further, a light microscopic investigation revealed that exposure to HgCl2 during gestation and lactation periods induced deleterious histopathological changes in the ovaries of mothers as well as in the ovaries and testes of their offspring. Additionally, the ovarian sections from mothers and their offspring displayed weak to negative expression for calretinin antibody, however, the testicular sections from offspring appeared strongly stained with COX-2 antibody (inflammatory marker) and weakly stained with Ki67 antibody (proliferative marker). Further study by flow cytometric technique revealed a highly significant increase in the mean % value of apoptotic cells in both ovaries and testes. On the other hand, co-supplementation of CE to HgCl 2 treated group successfully alleviated the altered antioxidants and hormones as well as the histological changes near to the normal as control. Conclusion : Celery extract has a powerful ameliorative role against HgCl 2 -induced deleterious biochemical and histopathological changes in the gonads of female rats and their pups.","PeriodicalId":406966,"journal":{"name":"Journal of Medical and Life Science","volume":"3 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2020-12-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"133786774","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2020-12-17DOI: 10.21608/jmals.2020.133543
Rasha Y M Ibrahim, H. Abdelhafez, F. Eid, Sawsan Abd EL-Maksoud ELShamy
Arabic gum (AG) is a natural branched-chain multifunctional hydrocolloid with a highly neutral or slightly acidic, arabinogalactan protein complex containing calcium, magnesium, and potassium. Arabic gum is a dried exudate obtained from the stem and branches of Acacia trees. AG has an anti-inflammatory and antioxidant effect. This study aimed to examine the possible anti-inflammatory and antioxidant effect of AG against the damage induced by alloxan injection on renal tissue of adult male rats using histological and histochemical studies. The current experiment was carried out on 48 adult male Albino rats. Rats were randomly and equally categorized into four groups 12 rats in each group as follows: 1) Control group (C): rats have left without treatment; 2) Diabetic groups (D): rats were injected with 150 mg/kg body weight of alloxan and left for 21 days ; 3) Arabic gum groups (G): rats were orally administrated AG (25 mg/kg body weight/day) for 21 days and 4) D+G groups: rats were injected with alloxan and treated with 25 mg/kg body weight/day for 21 days. The experimental rats were sacrificed after 7 and 21 days post-treatment. Examination of renal tissue of rats seven and twenty-one days post-alloxan injection revealed many histological and histochemical changes. Highly increased collagen fibres were demonstrated after seven and twenty-one days post alloxan injection. Also, alloxan injection significantly decreased PAS-positive materials, total protein content, and total DNA content, but it significantly increased amyloid β –protein content relative to the control group. While treatment with AG post-alloxan injection showed a trend toward lowering the incidence of renal tissue histological and histochemical changes induced by alloxan injection. According to the results obtained in the current study using AG as a natural agent showed a strong cytoprotective effect against the histological and histochemical changes due to its antioxidant effect.
{"title":"Arabic gum ameliorates histological and histochemical changes in the renal cortex of rats induced by alloxan injection","authors":"Rasha Y M Ibrahim, H. Abdelhafez, F. Eid, Sawsan Abd EL-Maksoud ELShamy","doi":"10.21608/jmals.2020.133543","DOIUrl":"https://doi.org/10.21608/jmals.2020.133543","url":null,"abstract":"Arabic gum (AG) is a natural branched-chain multifunctional hydrocolloid with a highly neutral or slightly acidic, arabinogalactan protein complex containing calcium, magnesium, and potassium. Arabic gum is a dried exudate obtained from the stem and branches of Acacia trees. AG has an anti-inflammatory and antioxidant effect. This study aimed to examine the possible anti-inflammatory and antioxidant effect of AG against the damage induced by alloxan injection on renal tissue of adult male rats using histological and histochemical studies. The current experiment was carried out on 48 adult male Albino rats. Rats were randomly and equally categorized into four groups 12 rats in each group as follows: 1) Control group (C): rats have left without treatment; 2) Diabetic groups (D): rats were injected with 150 mg/kg body weight of alloxan and left for 21 days ; 3) Arabic gum groups (G): rats were orally administrated AG (25 mg/kg body weight/day) for 21 days and 4) D+G groups: rats were injected with alloxan and treated with 25 mg/kg body weight/day for 21 days. The experimental rats were sacrificed after 7 and 21 days post-treatment. Examination of renal tissue of rats seven and twenty-one days post-alloxan injection revealed many histological and histochemical changes. Highly increased collagen fibres were demonstrated after seven and twenty-one days post alloxan injection. Also, alloxan injection significantly decreased PAS-positive materials, total protein content, and total DNA content, but it significantly increased amyloid β –protein content relative to the control group. While treatment with AG post-alloxan injection showed a trend toward lowering the incidence of renal tissue histological and histochemical changes induced by alloxan injection. According to the results obtained in the current study using AG as a natural agent showed a strong cytoprotective effect against the histological and histochemical changes due to its antioxidant effect.","PeriodicalId":406966,"journal":{"name":"Journal of Medical and Life Science","volume":"16 s1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2020-12-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"120858076","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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