Pub Date : 2023-07-11DOI: 10.21608/jmals.2023.307223
S. Hammood, Azhar Youns, Shaymaa Jaber, Zahraa S. Alkaabi
It is generally accepted that intrauterine insemination (IUI) with homologous semen should be preferred as a first choice treatment to more invasive and expensive techniques of assisted reproduction in the case of cervical, unexplained, and moderate male factor subfertility. Scientific validation of this strategy is difficult because the literature is rather confusing and not conclusive. The rationale for the use of artificial insemination is to increase gamete density at the site of fertilization. Many variables may influence success rates after IUI treatment procedures. Some factors will never be solved by methodological adjustments such as female age, duration of infertility, etc. However, quite some variables can be improved upon using different strategies.
{"title":"Review of Intrauterine insemination (IUI) treatment factors and influence on the success rates","authors":"S. Hammood, Azhar Youns, Shaymaa Jaber, Zahraa S. Alkaabi","doi":"10.21608/jmals.2023.307223","DOIUrl":"https://doi.org/10.21608/jmals.2023.307223","url":null,"abstract":"It is generally accepted that intrauterine insemination (IUI) with homologous semen should be preferred as a first choice treatment to more invasive and expensive techniques of assisted reproduction in the case of cervical, unexplained, and moderate male factor subfertility. Scientific validation of this strategy is difficult because the literature is rather confusing and not conclusive. The rationale for the use of artificial insemination is to increase gamete density at the site of fertilization. Many variables may influence success rates after IUI treatment procedures. Some factors will never be solved by methodological adjustments such as female age, duration of infertility, etc. However, quite some variables can be improved upon using different strategies.","PeriodicalId":406966,"journal":{"name":"Journal of Medical and Life Science","volume":"203 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-07-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"115796136","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-06-01DOI: 10.21608/jmals.2023.305704
Mustafa Kasid, R. AlChalabi, Faisal Harith, Ghydaa H. Aljeboury
The tetra-arm method was employed in this investigation to identify the SNP (rs2569190) in the promoter region of the CD14 gene as a risk factor for acquiring chronic urinary tract infections in a sample of Iraqis with a history of these illnesses in various forms. Using sandwich ELISA to estimate the serum level of CD14 in conjunction with TLR4 and other associated cytokines (TNF-α, TGF-β, and IFN-γ). A Gram-negative co-receptor for LPS, CD14 is expressed by innate immune cells. Through their effects on neutrophil expression levels, which have been associated with pyelonephritis, recurrent cystitis, and asymptomatic bacteriuria, its genetic variations have been connected to susceptibility to various types of UTIs The frequency of genotypes distribution and alleles of rs2569190 for two investigated groups presented The GG, GA, and AA genotype frequencies of rs2569190 were (26.1 %, 60.5 % and 13.4 %) respectively among the patients while 77.5 % for GG, 17.5% for GA and 5% for AA genotypes of healthy control.
{"title":"Association between Single Nucleotide Polymorphism in the Promoter of CD14 Gene and Development of Urinary Tract Infection","authors":"Mustafa Kasid, R. AlChalabi, Faisal Harith, Ghydaa H. Aljeboury","doi":"10.21608/jmals.2023.305704","DOIUrl":"https://doi.org/10.21608/jmals.2023.305704","url":null,"abstract":"The tetra-arm method was employed in this investigation to identify the SNP (rs2569190) in the promoter region of the CD14 gene as a risk factor for acquiring chronic urinary tract infections in a sample of Iraqis with a history of these illnesses in various forms. Using sandwich ELISA to estimate the serum level of CD14 in conjunction with TLR4 and other associated cytokines (TNF-α, TGF-β, and IFN-γ). A Gram-negative co-receptor for LPS, CD14 is expressed by innate immune cells. Through their effects on neutrophil expression levels, which have been associated with pyelonephritis, recurrent cystitis, and asymptomatic bacteriuria, its genetic variations have been connected to susceptibility to various types of UTIs The frequency of genotypes distribution and alleles of rs2569190 for two investigated groups presented The GG, GA, and AA genotype frequencies of rs2569190 were (26.1 %, 60.5 % and 13.4 %) respectively among the patients while 77.5 % for GG, 17.5% for GA and 5% for AA genotypes of healthy control.","PeriodicalId":406966,"journal":{"name":"Journal of Medical and Life Science","volume":"45 1 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"114345140","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-06-01DOI: 10.21608/jmals.2023.305596
S. Al-Sudani, H. Najim, Dheyaa Abdul Hussain
: Staphylococcus aureus has been subjected to low-level laser radiation at various exposure periods. From January 2019 to September 2019, 140 skin and UTI samples (swabs) were obtained from patients at Medical City and Medical City Teaching Hospital who had burns, wounds, and UTI s infections. 50 isolates of S. aureus were identified through cultural traits, microscopic examination, biochemical tests, API staph, and the Vitek system. The 15 isolates were subjected to a diode laser at an exposure length of 1 minute, 3 minutes, and 5 minutes. Before and after irradiation, it was evaluated how active the bacteria were in producing alpha toxin and how sensitive they were to antibiotics. The results of the alpha toxin generation of 15 irradiated isolates showed that the amount of toxin produced decreased at different irradiation exposure times [1, 3, and 5 min.]. The diode laser's impact on S. aureus' sensitivity to antibiotics results in a small increase in the diameter of the drugs' inhibition zone at various exposure times.
{"title":"A Comparative study of the laser beam effect on some virulence factors of Staphylococcus aureus isolated from different sites of the human body","authors":"S. Al-Sudani, H. Najim, Dheyaa Abdul Hussain","doi":"10.21608/jmals.2023.305596","DOIUrl":"https://doi.org/10.21608/jmals.2023.305596","url":null,"abstract":": Staphylococcus aureus has been subjected to low-level laser radiation at various exposure periods. From January 2019 to September 2019, 140 skin and UTI samples (swabs) were obtained from patients at Medical City and Medical City Teaching Hospital who had burns, wounds, and UTI s infections. 50 isolates of S. aureus were identified through cultural traits, microscopic examination, biochemical tests, API staph, and the Vitek system. The 15 isolates were subjected to a diode laser at an exposure length of 1 minute, 3 minutes, and 5 minutes. Before and after irradiation, it was evaluated how active the bacteria were in producing alpha toxin and how sensitive they were to antibiotics. The results of the alpha toxin generation of 15 irradiated isolates showed that the amount of toxin produced decreased at different irradiation exposure times [1, 3, and 5 min.]. The diode laser's impact on S. aureus' sensitivity to antibiotics results in a small increase in the diameter of the drugs' inhibition zone at various exposure times.","PeriodicalId":406966,"journal":{"name":"Journal of Medical and Life Science","volume":"1 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"129890947","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-06-01DOI: 10.21608/jmals.2023.305819
P. Waithaka, B. Githaiga
Objectives: The indiscriminate use of antibiotics has led to an upsurge in antibiotic resistance in many parts of the world. This study aimed at determining the seasonal variation of antibiotic-producing actinomycetes from Menengai crater, Kenya. Methods: Soil samples were collected in February which receives 52mm of rainfall, July (100mm), and May (194mm). Actinomycetes were isolated using serial dilution and spread plate bioassays. The antibiotic property of the isolates against Staphylococcus aureus (ATCC 25923), Streptococcus pneumoniae (ATCC 49617), Enterococcus faecalis (ATCC 29212) , Escherichia coli (ATCC 25922) , Pseudomonas aeruginosa (ATCC 27853) and Proteus vulgaris (ATCC 49990) was determined using primary and secondary screening methods. Based on the size of the zone of inhibition and a broad spectrum of activity, four actinomyces coded PAN 25, 41, 75, and 110 were selected for further analysis. Results: The physicochemical properties of the soil samples did not vary significantly (P=0.42). However, the number of actinomycetes isolated varied significantly (P=0.035) between February, May, and July. The mean zone of inhibition varied between 22±0.3 in PAN 110 to 27±0.3mm in PAN 75. Isolate PAN 110 produced an 829bp fragment, PAN 75 (971bp), PAN 41 (1250bp), and PAN 110 (829bp). The 16S rRNA gene sequence and nucleotide BLAST from NCBI revealed that isolate PAN 110 had 99% similarity with S. acrimycini strain K30, PAN 75 ( S. luteogriseus strain ZG728), PAN 41 ( S. indiaensis ), and PAN 25 ( Streptomyces variabilis ). Conclusion: Very dry and wet conditions reduced the number of actinomycetes isolated from the soils of the Menengai crater.
目标:滥用抗生素已导致世界许多地区抗生素耐药性激增。本研究旨在确定肯尼亚Menengai火山口产抗生素放线菌的季节变化。方法:2月(52mm)、7月(100mm)、5月(194mm)采集土壤样品。采用连续稀释法和涂布平板法分离放线菌。采用一级筛选和二级筛选的方法检测分离株对金黄色葡萄球菌(ATCC 25923)、肺炎链球菌(ATCC 49617)、粪肠球菌(ATCC 29212)、大肠埃希菌(ATCC 25922)、铜绿假单胞菌(ATCC 27853)和寻常变形杆菌(ATCC 49990)的抗菌性能。根据抑制区大小和较宽的活性谱，选择编码为PAN 25、41、75和110的4种放线菌进行进一步分析。结果:土壤样品理化性质无显著差异(P=0.42)。2月、5月和7月放线菌分离数量差异显著(P=0.035)。PAN 110的平均抑制区为22±0.3 mm, PAN 75的平均抑制区为27±0.3mm。PAN 110分离得到829bp的片段，PAN 75 (971bp)， PAN 41 (1250bp)和PAN 110 (829bp)。NCBI的16S rRNA基因序列和核苷酸BLAST分析结果显示，分离物PAN 110与S. acrimycini菌株K30、S. luteogriseus菌株ZG728、S. indiaensis菌株PAN 41和变异链霉菌pan25具有99%的相似性。结论:非常干燥和潮湿的环境使门能盖陨石坑土壤中放线菌的分离数量减少。
{"title":"Seasonal variation of antibiotic-producing actinomycetes from Menengai crater, Kenya","authors":"P. Waithaka, B. Githaiga","doi":"10.21608/jmals.2023.305819","DOIUrl":"https://doi.org/10.21608/jmals.2023.305819","url":null,"abstract":"Objectives: The indiscriminate use of antibiotics has led to an upsurge in antibiotic resistance in many parts of the world. This study aimed at determining the seasonal variation of antibiotic-producing actinomycetes from Menengai crater, Kenya. Methods: Soil samples were collected in February which receives 52mm of rainfall, July (100mm), and May (194mm). Actinomycetes were isolated using serial dilution and spread plate bioassays. The antibiotic property of the isolates against Staphylococcus aureus (ATCC 25923), Streptococcus pneumoniae (ATCC 49617), Enterococcus faecalis (ATCC 29212) , Escherichia coli (ATCC 25922) , Pseudomonas aeruginosa (ATCC 27853) and Proteus vulgaris (ATCC 49990) was determined using primary and secondary screening methods. Based on the size of the zone of inhibition and a broad spectrum of activity, four actinomyces coded PAN 25, 41, 75, and 110 were selected for further analysis. Results: The physicochemical properties of the soil samples did not vary significantly (P=0.42). However, the number of actinomycetes isolated varied significantly (P=0.035) between February, May, and July. The mean zone of inhibition varied between 22±0.3 in PAN 110 to 27±0.3mm in PAN 75. Isolate PAN 110 produced an 829bp fragment, PAN 75 (971bp), PAN 41 (1250bp), and PAN 110 (829bp). The 16S rRNA gene sequence and nucleotide BLAST from NCBI revealed that isolate PAN 110 had 99% similarity with S. acrimycini strain K30, PAN 75 ( S. luteogriseus strain ZG728), PAN 41 ( S. indiaensis ), and PAN 25 ( Streptomyces variabilis ). Conclusion: Very dry and wet conditions reduced the number of actinomycetes isolated from the soils of the Menengai crater.","PeriodicalId":406966,"journal":{"name":"Journal of Medical and Life Science","volume":"26 11","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"132644625","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-06-01DOI: 10.21608/jmals.2023.305080
A. Yameny
The COVID-19 pandemic is characterized by the highly infectious severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), Omicron variant, which was first reported in Botswana and quickly spread as the dominant variant worldwide during early 2022, the start of 2023 brings a new subvariant of the Omicron variant of SARS-CoV-2, known as XBB.1.5. The Omicron XBB.1.5 subvariant is a sublineage of the XBB variant, a recombinant of two BA.2 sublineages, with an additional spike receptor-binding domain (RBD) change, S486P. It is demonstrated that XBB.1.5 is just as immunologically evasive as XBB.1, the Omicron subvariant with the largest immune escape to date, using pseudotyped viral neutralization experiments. There is currently no data on real-world vaccine effectiveness against severe disease or death. At this time, there are no indications that XBB.1.5's infection severity differs from that of earlier Omicron sub-lineages that were circulating. The gold standard for COVID-19 diagnosis is reverse transcription-quantitative polymerase chain reaction (RT-qPCR) testing using upper respiratory tract swabs or saliva. there are no vaccine effectiveness (VE) estimates for XBB.1.5 yet, no specific data are currently available on the effectiveness of COVID-19 antiviral therapeutics.
{"title":"Characterization of SARS-CoV-2 Omicron XBB.1.5 sub-lineage: A review","authors":"A. Yameny","doi":"10.21608/jmals.2023.305080","DOIUrl":"https://doi.org/10.21608/jmals.2023.305080","url":null,"abstract":"The COVID-19 pandemic is characterized by the highly infectious severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), Omicron variant, which was first reported in Botswana and quickly spread as the dominant variant worldwide during early 2022, the start of 2023 brings a new subvariant of the Omicron variant of SARS-CoV-2, known as XBB.1.5. The Omicron XBB.1.5 subvariant is a sublineage of the XBB variant, a recombinant of two BA.2 sublineages, with an additional spike receptor-binding domain (RBD) change, S486P. It is demonstrated that XBB.1.5 is just as immunologically evasive as XBB.1, the Omicron subvariant with the largest immune escape to date, using pseudotyped viral neutralization experiments. There is currently no data on real-world vaccine effectiveness against severe disease or death. At this time, there are no indications that XBB.1.5's infection severity differs from that of earlier Omicron sub-lineages that were circulating. The gold standard for COVID-19 diagnosis is reverse transcription-quantitative polymerase chain reaction (RT-qPCR) testing using upper respiratory tract swabs or saliva. there are no vaccine effectiveness (VE) estimates for XBB.1.5 yet, no specific data are currently available on the effectiveness of COVID-19 antiviral therapeutics.","PeriodicalId":406966,"journal":{"name":"Journal of Medical and Life Science","volume":"137 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"133099078","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-05-22DOI: 10.21608/jmals.2023.299892
Kassab Mohammed
{"title":"Recombinant oral human insulin tablets preparation","authors":"Kassab Mohammed","doi":"10.21608/jmals.2023.299892","DOIUrl":"https://doi.org/10.21608/jmals.2023.299892","url":null,"abstract":"","PeriodicalId":406966,"journal":{"name":"Journal of Medical and Life Science","volume":"1 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-05-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"130309767","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-05-22DOI: 10.21608/jmals.2023.299895
{"title":"Development of Hepatitis C virus-like particles vaccine against genotype 4. predominating in Egypt by bioinformatics","authors":"","doi":"10.21608/jmals.2023.299895","DOIUrl":"https://doi.org/10.21608/jmals.2023.299895","url":null,"abstract":"","PeriodicalId":406966,"journal":{"name":"Journal of Medical and Life Science","volume":"12 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-05-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"124623131","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-05-20DOI: 10.21608/jmals.2023.299646
{"title":"Beyond Conventional Treatment: Exploring Natural Therapies for Paracetamol Overdose","authors":"","doi":"10.21608/jmals.2023.299646","DOIUrl":"https://doi.org/10.21608/jmals.2023.299646","url":null,"abstract":"","PeriodicalId":406966,"journal":{"name":"Journal of Medical and Life Science","volume":"os-13 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-05-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"127763979","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-03-01DOI: 10.21608/jmals.2023.295412
M. Kassab
{"title":"Production and screening of fungal L-asparaginase enzymes as anticancer agents from high-contrast soil environments in Egypt","authors":"M. Kassab","doi":"10.21608/jmals.2023.295412","DOIUrl":"https://doi.org/10.21608/jmals.2023.295412","url":null,"abstract":"","PeriodicalId":406966,"journal":{"name":"Journal of Medical and Life Science","volume":"10 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"128554332","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-03-01DOI: 10.21608/jmals.2023.295728
Nourhan Fekry, EL- Sayed Abd Elmaqsoud, hosny elewa, Mahasin Abdel Rhman, Karema Abu-Elfotuh, Z. Zalat
{"title":"Direct Acting Anti-Viral Therapy, and Potential Increase in the Incidence of Hepato-Cellular Carcinoma in Cirrhotic Patients With Hepatitis C Virus","authors":"Nourhan Fekry, EL- Sayed Abd Elmaqsoud, hosny elewa, Mahasin Abdel Rhman, Karema Abu-Elfotuh, Z. Zalat","doi":"10.21608/jmals.2023.295728","DOIUrl":"https://doi.org/10.21608/jmals.2023.295728","url":null,"abstract":"","PeriodicalId":406966,"journal":{"name":"Journal of Medical and Life Science","volume":"97 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"123065425","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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