Pub Date : 2024-02-05DOI: 10.21608/jmals.2024.260653.1016
Othman Othman, Sahar Kamal
{"title":"Efficacy and safety of Tenofovir Disoproxil Fumarate in Egyptian patients with chronic hepatitis B virus infection","authors":"Othman Othman, Sahar Kamal","doi":"10.21608/jmals.2024.260653.1016","DOIUrl":"https://doi.org/10.21608/jmals.2024.260653.1016","url":null,"abstract":"","PeriodicalId":406966,"journal":{"name":"Journal of Medical and Life Science","volume":"42 3","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-02-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139863880","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-02-05DOI: 10.21608/jmals.2024.260653.1016
Othman Othman, Sahar Kamal
{"title":"Efficacy and safety of Tenofovir Disoproxil Fumarate in Egyptian patients with chronic hepatitis B virus infection","authors":"Othman Othman, Sahar Kamal","doi":"10.21608/jmals.2024.260653.1016","DOIUrl":"https://doi.org/10.21608/jmals.2024.260653.1016","url":null,"abstract":"","PeriodicalId":406966,"journal":{"name":"Journal of Medical and Life Science","volume":"2 19","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-02-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139804063","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-01-13DOI: 10.21608/jmals.2024.336113
Aml Ghoneim, Sara Abdou, Emad Elsehly, Eman Radwan, Aml Abdelrahman, Nada Badr
Background : Chitosan oligosaccharide (COS) exhibits hypolipidemic and anti-obesity properties. Nevertheless, it remains uncertain whether its efficiency exhibits sex-specific variations. Therefore, this study seeks to fill this gap, systematically evaluating the impact of chitosan oligosaccharides across genders under obesogenic conditions. Material and methods : 36 rats: 18 male, 18 female; split into 6 groups, each with equal male/female. G1 had a standard diet; G2 had a high-fat diet for 4 weeks, then standard for another 4 weeks; G3 had a high-fat diet for 4 weeks, then 500 mg/kg chitosan + standard diet for another 4 weeks. At the study's end, rats were euthanized, blood was collected for biochemical analysis, and liver tissues were excised for histological assessment. Results : Male rats on a high-fat diet displayed significantly elevated body weight, triglycerides, cholesterol, lactate dehydrogenase, ALT, AST, and total protein levels, coupled with noticeable liver damage compared to female rats. However, when treated with COS, male and female rats exhibited reduced weight, lowered triglycerides, cholesterol, lactate dehydrogenase, ALT, and AST levels, and improved liver function and structure. Interestingly, these therapeutic effects were more evident in female and male rats. Conclusion: The study found that COS administration to rats, especially females, reduced weight, and improved liver health, suggesting its potential gender-specific effects on a high-fat diet.
背景：壳聚糖低聚糖（COS）具有降血脂和抗肥胖的功效。然而，其功效是否存在性别差异仍不确定。因此，本研究试图填补这一空白，系统评估壳聚糖低聚糖在致肥条件下对不同性别的影响。材料和方法：36 只大鼠：雄性 18 只，雌性 18 只；分为 6 组，每组雌雄比例相等。G1 组食用标准食物；G2 组食用高脂肪食物 4 周，然后食用标准食物 4 周；G3 组食用高脂肪食物 4 周，然后食用 500 毫克/千克壳聚糖 + 标准食物 4 周。研究结束后，对大鼠实施安乐死，采集血液进行生化分析，并切除肝脏组织进行组织学评估。结果：与雌性大鼠相比，摄入高脂肪食物的雄性大鼠体重、甘油三酯、胆固醇、乳酸脱氢酶、谷丙转氨酶、谷草转氨酶和总蛋白水平明显升高，肝脏明显受损。然而，在使用 COS 治疗后，雄性和雌性大鼠的体重都减轻了，甘油三酯、胆固醇、乳酸脱氢酶、谷丙转氨酶和谷草转氨酶水平降低了，肝功能和结构也得到了改善。有趣的是，这些治疗效果在雌性和雄性大鼠身上更为明显。结论研究发现，给大鼠（尤其是雌性大鼠）服用 COS 可减轻体重，改善肝脏健康，这表明 COS 对高脂肪饮食有潜在的性别特异性作用。
{"title":"Evaluating the Anti-Obesity Effects of Chitosan in High-Fat Diet-Induced Obesity in Rat Model","authors":"Aml Ghoneim, Sara Abdou, Emad Elsehly, Eman Radwan, Aml Abdelrahman, Nada Badr","doi":"10.21608/jmals.2024.336113","DOIUrl":"https://doi.org/10.21608/jmals.2024.336113","url":null,"abstract":"Background : Chitosan oligosaccharide (COS) exhibits hypolipidemic and anti-obesity properties. Nevertheless, it remains uncertain whether its efficiency exhibits sex-specific variations. Therefore, this study seeks to fill this gap, systematically evaluating the impact of chitosan oligosaccharides across genders under obesogenic conditions. Material and methods : 36 rats: 18 male, 18 female; split into 6 groups, each with equal male/female. G1 had a standard diet; G2 had a high-fat diet for 4 weeks, then standard for another 4 weeks; G3 had a high-fat diet for 4 weeks, then 500 mg/kg chitosan + standard diet for another 4 weeks. At the study's end, rats were euthanized, blood was collected for biochemical analysis, and liver tissues were excised for histological assessment. Results : Male rats on a high-fat diet displayed significantly elevated body weight, triglycerides, cholesterol, lactate dehydrogenase, ALT, AST, and total protein levels, coupled with noticeable liver damage compared to female rats. However, when treated with COS, male and female rats exhibited reduced weight, lowered triglycerides, cholesterol, lactate dehydrogenase, ALT, and AST levels, and improved liver function and structure. Interestingly, these therapeutic effects were more evident in female and male rats. Conclusion: The study found that COS administration to rats, especially females, reduced weight, and improved liver health, suggesting its potential gender-specific effects on a high-fat diet.","PeriodicalId":406966,"journal":{"name":"Journal of Medical and Life Science","volume":"23 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-01-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139530669","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-01-11DOI: 10.21608/jmals.2024.335787
A. Alazbjee, N. Ali, Zainab farhan
Objective: In this research paper, we examine how treating patients with type 1 and type 2 diabetes with vitamin B12 and magnesium affects both insulin and non-insulin levels. The Glycated hemoglobin (HbA1c) marker, which is one of the fundamental and clinical markers to monitor in the control of type 2 diabetes mellitus patients (T2DM and type 1 diabetic mellitus), is also highlighted and discussed in this study. Methods: From February to March 2023, a cross-sectional study was done at the Medical Labs Research Center of Al-Hussein Teaching Hospital in Nasiriyah Governorate. Our research included 40 people with type 1 and type 2 diabetes. Before fasting, a physical examination, a thorough medical history, and blood samples were obtained. Following a 12-hour fast, all blood samples were collected. Results: Except for the B12 and Mg ++ levels being significantly higher in DM1 patients in both the first and second age groups and the third age group of DM1 patients being significantly lower at p. value 0.05, we conclude from this study's two-way ANOVA test and t-test that cumulative sugar is unaffected by age or type of sugar. Conclusions: Our findings indicate that both concentrations of B 12 and Mg ++ increased significantly in patients with DM 1 than DM 2 at p. value < 0.05. These findings call for more research on a wider population to look at the source of the deficit and the effectiveness of B12 supplementation in these patients.
{"title":"A pathochemical study to assess the level of vitamin B12 and magnesium in patients with type 1 and type 2 diabetes mellitus in Thi-Qar governorate.","authors":"A. Alazbjee, N. Ali, Zainab farhan","doi":"10.21608/jmals.2024.335787","DOIUrl":"https://doi.org/10.21608/jmals.2024.335787","url":null,"abstract":"Objective: In this research paper, we examine how treating patients with type 1 and type 2 diabetes with vitamin B12 and magnesium affects both insulin and non-insulin levels. The Glycated hemoglobin (HbA1c) marker, which is one of the fundamental and clinical markers to monitor in the control of type 2 diabetes mellitus patients (T2DM and type 1 diabetic mellitus), is also highlighted and discussed in this study. Methods: From February to March 2023, a cross-sectional study was done at the Medical Labs Research Center of Al-Hussein Teaching Hospital in Nasiriyah Governorate. Our research included 40 people with type 1 and type 2 diabetes. Before fasting, a physical examination, a thorough medical history, and blood samples were obtained. Following a 12-hour fast, all blood samples were collected. Results: Except for the B12 and Mg ++ levels being significantly higher in DM1 patients in both the first and second age groups and the third age group of DM1 patients being significantly lower at p. value 0.05, we conclude from this study's two-way ANOVA test and t-test that cumulative sugar is unaffected by age or type of sugar. Conclusions: Our findings indicate that both concentrations of B 12 and Mg ++ increased significantly in patients with DM 1 than DM 2 at p. value < 0.05. These findings call for more research on a wider population to look at the source of the deficit and the effectiveness of B12 supplementation in these patients.","PeriodicalId":406966,"journal":{"name":"Journal of Medical and Life Science","volume":"34 14","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-01-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139533887","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-11-16DOI: 10.21608/jmals.2023.326295
Olfat Elghareeb, Hanem El-sherbiny, Najamul Nisa, Rania Abou-El-magd
Background: One well-known hepatotoxin that is frequently used to cause acute toxic liver injury in a variety of experimental animals is carbon tetrachloride (CCl4). There have been suggestions that ginger (Zingiber officinale) offers great promise in alleviation of many diseases . Aim: The purpose of this study was to evaluate the ameliorative role of ginger extract (GE) against the liver damage induced by CCL4 in male rats. Material &methods: Thirty-two male adult albino rats were used in this study. The rats were divided into four groups (N=8): control group, GE-supplemented group (200mg / kg b.wt daily for consecutive 35 days), CCL4-treated group (2mg/kg b.wt/twice weekly for 5 weeks), and CCL4-intoxicated group followed by treatment with GE. Results : GE for consecutive 35 days significantly reduced the adverse effects of CCL4 toxicity on the levels of liver enzymes (ALT, AST, and ALP), total bilirubin, total protein, and albumin. The altered liver tissue antioxidants (SOD, CAT, and GPx) and MDA were significantly ameliorated on treatment with GE. Additionally, the liver histopathological alterations induced by CCL4 were markedly recurred on treatment with GE. Conclusion: Ginger extract has a potential ameliorative role against CCL4-induced adverse biochemical and histological alterations in the liver of male rats
背景：四氯化碳（CCl4）是一种众所周知的肝脏毒素，经常被用来对各种实验动物造成急性中毒性肝损伤。有人认为，生姜（Zingiber officinale）在缓解多种疾病方面大有可为。目的：本研究旨在评估生姜提取物（GE）对 CCL4 诱导的雄性大鼠肝损伤的改善作用。材料与方法：本研究使用了 32 只成年雄性白化大鼠。将大鼠分为四组（N=8）：对照组、添加 GE 组（每天 200 毫克/千克体重，连续 35 天）、CCL4 治疗组（每周两次，每次 2 毫克/千克体重，连续 5 周）和 CCL4 中毒后再用 GE 治疗的组。结果：连续 35 天使用 GE 能明显减轻 CCL4 毒性对肝酶（谷丙转氨酶、谷草转氨酶和谷草转氨酶）、总胆红素、总蛋白和白蛋白水平的不良影响。使用 GE 治疗后，肝组织抗氧化剂（SOD、CAT 和 GPx）和 MDA 的变化明显改善。此外，CCL4 诱导的肝组织病理学改变在使用 GE 治疗后明显复发。结论生姜提取物对 CCL4 诱导的雄性大鼠肝脏不良生化和组织学改变具有潜在的改善作用
{"title":"Evaluation of the ameliorative role of ginger (Zingiber officinale) extract against carbon tetrachloride-induced hepatotoxicity in male albino rats","authors":"Olfat Elghareeb, Hanem El-sherbiny, Najamul Nisa, Rania Abou-El-magd","doi":"10.21608/jmals.2023.326295","DOIUrl":"https://doi.org/10.21608/jmals.2023.326295","url":null,"abstract":"Background: One well-known hepatotoxin that is frequently used to cause acute toxic liver injury in a variety of experimental animals is carbon tetrachloride (CCl4). There have been suggestions that ginger (Zingiber officinale) offers great promise in alleviation of many diseases . Aim: The purpose of this study was to evaluate the ameliorative role of ginger extract (GE) against the liver damage induced by CCL4 in male rats. Material &methods: Thirty-two male adult albino rats were used in this study. The rats were divided into four groups (N=8): control group, GE-supplemented group (200mg / kg b.wt daily for consecutive 35 days), CCL4-treated group (2mg/kg b.wt/twice weekly for 5 weeks), and CCL4-intoxicated group followed by treatment with GE. Results : GE for consecutive 35 days significantly reduced the adverse effects of CCL4 toxicity on the levels of liver enzymes (ALT, AST, and ALP), total bilirubin, total protein, and albumin. The altered liver tissue antioxidants (SOD, CAT, and GPx) and MDA were significantly ameliorated on treatment with GE. Additionally, the liver histopathological alterations induced by CCL4 were markedly recurred on treatment with GE. Conclusion: Ginger extract has a potential ameliorative role against CCL4-induced adverse biochemical and histological alterations in the liver of male rats","PeriodicalId":406966,"journal":{"name":"Journal of Medical and Life Science","volume":"5 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-11-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139267836","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-08-17DOI: 10.21608/jmals.2023.313283
Salama S. Salama, Abdel daim J., A. Saleh
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{"title":"EFFECT OF MAGNETIC WATER ON SOME ACTIVITIES OF HONEY BEES (Apis mellifera L.) in Jazan Province KSA","authors":"Salama S. Salama, Abdel daim J., A. Saleh","doi":"10.21608/jmals.2023.313283","DOIUrl":"https://doi.org/10.21608/jmals.2023.313283","url":null,"abstract":".","PeriodicalId":406966,"journal":{"name":"Journal of Medical and Life Science","volume":"32 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-08-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"114461640","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-07-21DOI: 10.21608/jmals.2023.309050
Haidar Alsaedi
: The Rationale Behind the Evaluation Mitral valvular disease, which includes mitral stenosis and mitral regurgitation, is the focus of this review. The structure of the valve is our starting point for this discussion. According to recent studies, 3D imaging has made it possible to better understand valve anatomy. Percutaneous balloon mitral valvuloplasty is still the best treatment for mitral stenosis when necessary and possible based on the patient's anatomy. The most common cause of mitral stenosis is atherosclerosis. Changes in the left ventricle's shape can lead to mitral regurgitation (MR), which can be caused by a problem in the mitral apparatus or the aortic valve. Researchers are exploring percutaneous methods of mitral valve repair and replacement, but surgery is still the gold standard for treating severe primary MR. The disease of the mitral valve is a common medical issue. If you want to provide the best care for a patient, you need to have a thorough understanding of the mitral valve and the diseases that can damage it.
{"title":"Study of Mitral Valve Insufficiency: A Systemic Review","authors":"Haidar Alsaedi","doi":"10.21608/jmals.2023.309050","DOIUrl":"https://doi.org/10.21608/jmals.2023.309050","url":null,"abstract":": The Rationale Behind the Evaluation Mitral valvular disease, which includes mitral stenosis and mitral regurgitation, is the focus of this review. The structure of the valve is our starting point for this discussion. According to recent studies, 3D imaging has made it possible to better understand valve anatomy. Percutaneous balloon mitral valvuloplasty is still the best treatment for mitral stenosis when necessary and possible based on the patient's anatomy. The most common cause of mitral stenosis is atherosclerosis. Changes in the left ventricle's shape can lead to mitral regurgitation (MR), which can be caused by a problem in the mitral apparatus or the aortic valve. Researchers are exploring percutaneous methods of mitral valve repair and replacement, but surgery is still the gold standard for treating severe primary MR. The disease of the mitral valve is a common medical issue. If you want to provide the best care for a patient, you need to have a thorough understanding of the mitral valve and the diseases that can damage it.","PeriodicalId":406966,"journal":{"name":"Journal of Medical and Life Science","volume":"46 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-07-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"128285373","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-07-17DOI: 10.21608/jmals.2023.308224
Aml Talaat, Yomna A. Elgendy, Heba F. Mohamed, Nouran M Saed, Noran A. Abd Elrouf, Hend A. Elgendy, Hagar H. Elbalakousy, Nourhan N. El hantoshy, Mohammed S. Elmezaien, Youssef M. Sayed, Yosry El. Hekal, M. Gabr, Nada S. Badr
Paracetamol is a widely used analgesic and antipyretic drug, but its long-term usage has been associated with potential toxicity. The goal of this study was to investigate how frankincense oil prevents paracetamol toxicity. The study included twenty male albino rats divided into four groups: (G1) control, (G2) paracetamol, (G3) frankincense oil, and (G4) paracetamol + frankincense oil. For three days, 1000 mg/kg paracetamol was given orally, while frankincense oil was given orally at a dosage of 1000 mg/kg concurrently with paracetamol. Hematological parameters, lipid profile, cardiac markers, pancreatic function, blood antioxidant capacity, myeloperoxidase activity, blood, spleen, pancreas, heart, and lung histopathology were evaluated. Paracetamol caused oxidative stress, hematotoxicity, dyslipidemia, myocardial damage, pancreatic dysfunction, spleen alterations, and lung tissue damage. However, coadministration of paracetamol and frankincense oil protected against these effects by boosting antioxidant capacity, improving hematological parameters, preserving pancreatic function, enhancing lipid profiles, preserving spleen morphology, and preventing lung damage, likely due to the antioxidant properties of frankincense oil.
{"title":"Ameliorative effects of frankincense oil on rats treated with a minimum toxic dose of paracetamol","authors":"Aml Talaat, Yomna A. Elgendy, Heba F. Mohamed, Nouran M Saed, Noran A. Abd Elrouf, Hend A. Elgendy, Hagar H. Elbalakousy, Nourhan N. El hantoshy, Mohammed S. Elmezaien, Youssef M. Sayed, Yosry El. Hekal, M. Gabr, Nada S. Badr","doi":"10.21608/jmals.2023.308224","DOIUrl":"https://doi.org/10.21608/jmals.2023.308224","url":null,"abstract":"Paracetamol is a widely used analgesic and antipyretic drug, but its long-term usage has been associated with potential toxicity. The goal of this study was to investigate how frankincense oil prevents paracetamol toxicity. The study included twenty male albino rats divided into four groups: (G1) control, (G2) paracetamol, (G3) frankincense oil, and (G4) paracetamol + frankincense oil. For three days, 1000 mg/kg paracetamol was given orally, while frankincense oil was given orally at a dosage of 1000 mg/kg concurrently with paracetamol. Hematological parameters, lipid profile, cardiac markers, pancreatic function, blood antioxidant capacity, myeloperoxidase activity, blood, spleen, pancreas, heart, and lung histopathology were evaluated. Paracetamol caused oxidative stress, hematotoxicity, dyslipidemia, myocardial damage, pancreatic dysfunction, spleen alterations, and lung tissue damage. However, coadministration of paracetamol and frankincense oil protected against these effects by boosting antioxidant capacity, improving hematological parameters, preserving pancreatic function, enhancing lipid profiles, preserving spleen morphology, and preventing lung damage, likely due to the antioxidant properties of frankincense oil.","PeriodicalId":406966,"journal":{"name":"Journal of Medical and Life Science","volume":"1 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-07-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"131208489","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-07-15DOI: 10.21608/jmals.2023.307798
K. El-Said, Fayza Ali, Amro E Mohamed
Limitations of chemotherapeutic drugs for cancer treatment could be due to the lack of specificity, rapid drug metabolism, and harmful side effects. Therefore, finding new approaches in neoplastic cancer or targeting drugs depends on the pathways and characteristics of different tumor entities. Natural elements as anticancer agents are an effective strategy in the fight against cancer. This study investigated the biochemical and molecular mechanisms of Annona sp fruits extract (ASFE) in Ehrlich ascites carcinoma (EAC)-bearing mice. Seventy mice were divided into seven groups (n = 10) as follows: Gp1 was used as a negative control, from Gp2 to Gp7 were inoculated with 1 × 10 6 EAC-cells/mouse, then Gp2 left as EAC-bearing mice, Gp3 was injected with Cis (2 mg/kg), Gp4 was injected with ASFE (200 mg/kg), Gp5 was co-treated with Cis as Gp3 and ASFE as Gp4. Gp6 was injected with a low dose of Cis (0.5 mg/kg), and Gp7 was co-treated with a low dose of Cis as Gp6 and ASFE as Gp4. The body weight change percentages (b.wt%) were calculated. On day 14, all groups were sacrificed, the ascitic fluids were harvested, and the total tumor volume, count, and live and dead tumor cells were measured. The relative expression of P53, Bcl-2, BAX, and caspase-9 genes was determined in EAC cells by RT-PCR. Sera samples were collected for biochemical parameters assessment. Liver tissues were collected for the determination of oxidants/antioxidants biomarkers. The results showed that co-treatment of the high or low doses of Cis with ASFE led to synergistic effects via targeting apoptosis in EAC-cells that can significantly inhibit tumor growth, decrease liver dysfunctions induced by Cis and enhance the hepatic antioxidant status.
{"title":"Biochemical and molecular mechanisms of Annona sp. fruits extract in Ehrlich ascites carcinoma-bearing mice","authors":"K. El-Said, Fayza Ali, Amro E Mohamed","doi":"10.21608/jmals.2023.307798","DOIUrl":"https://doi.org/10.21608/jmals.2023.307798","url":null,"abstract":"Limitations of chemotherapeutic drugs for cancer treatment could be due to the lack of specificity, rapid drug metabolism, and harmful side effects. Therefore, finding new approaches in neoplastic cancer or targeting drugs depends on the pathways and characteristics of different tumor entities. Natural elements as anticancer agents are an effective strategy in the fight against cancer. This study investigated the biochemical and molecular mechanisms of Annona sp fruits extract (ASFE) in Ehrlich ascites carcinoma (EAC)-bearing mice. Seventy mice were divided into seven groups (n = 10) as follows: Gp1 was used as a negative control, from Gp2 to Gp7 were inoculated with 1 × 10 6 EAC-cells/mouse, then Gp2 left as EAC-bearing mice, Gp3 was injected with Cis (2 mg/kg), Gp4 was injected with ASFE (200 mg/kg), Gp5 was co-treated with Cis as Gp3 and ASFE as Gp4. Gp6 was injected with a low dose of Cis (0.5 mg/kg), and Gp7 was co-treated with a low dose of Cis as Gp6 and ASFE as Gp4. The body weight change percentages (b.wt%) were calculated. On day 14, all groups were sacrificed, the ascitic fluids were harvested, and the total tumor volume, count, and live and dead tumor cells were measured. The relative expression of P53, Bcl-2, BAX, and caspase-9 genes was determined in EAC cells by RT-PCR. Sera samples were collected for biochemical parameters assessment. Liver tissues were collected for the determination of oxidants/antioxidants biomarkers. The results showed that co-treatment of the high or low doses of Cis with ASFE led to synergistic effects via targeting apoptosis in EAC-cells that can significantly inhibit tumor growth, decrease liver dysfunctions induced by Cis and enhance the hepatic antioxidant status.","PeriodicalId":406966,"journal":{"name":"Journal of Medical and Life Science","volume":"29 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-07-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"132567770","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-07-13DOI: 10.21608/jmals.2023.307496
Hany Desouky, N. Rizk, Huda Said, Reham Abd El-Azeem
Background: Pesticides are agrochemicals extensively used all over the world for pest control and prevention, Egyptian farmers are occupationally exposed to many types of pesticides, making them at high risk for pesticide toxicity. Several studies have shown acute pesticide poisoning effects, but fewer have dealt with chronic pesticide toxicity, especially studies regarding pesticide's effects on hematological parameters. Objective: We aimed to evaluate hematological parameter changes related to long-term low-level pesticide exposure. Subjects and Methods: The blood samples were collected from 96 Egyptian farmers chronically exposed to mixed pesticides and 54 nonexposed controls. A Complete Blood Picture (CBC) was evaluated to explore the hematological indices alterations. Statistical analysis was conducted using IBM SPSS Statistics, version 25. Results: A significant decrease in hemoglobin, hematocrit, mean corpuscular volume, and mean corpuscular hemoglobin in addition to platelet count in the exposed farmers compared to the control, On the other hand, white blood cell count was significantly increased. Conclusion: Chronic occupational pesticide exposure of Egyptian farmers leads to a range of disorders in hematological indices. As an affordable and readily available test, the Complete Blood Picture can help in the chronic monitoring of farmers health status who are regularly exposed to pesticides.
背景:农药是世界各地广泛用于防治病虫害的农用化学品，埃及农民在职业上接触多种农药，农药毒性风险很高。一些研究显示了农药的急性中毒效应，但较少涉及农药的慢性毒性，特别是农药对血液参数的影响的研究。目的:评价长期低剂量农药暴露对血液参数的影响。对象和方法:采集了96名长期暴露于混合农药的埃及农民和54名未暴露的对照者的血液样本。评估全血图(CBC)以探讨血液学指标的改变。采用IBM SPSS Statistics, version 25进行统计分析。结果:与对照组相比，暴露农民血红蛋白、红细胞压积、平均红细胞体积、平均红细胞血红蛋白和血小板计数均显著降低，白细胞计数显著增加。结论:埃及农民长期职业性农药暴露导致一系列血液学指标紊乱。作为一种负担得起且容易获得的测试，全血图可以帮助长期监测经常接触农药的农民的健康状况。
{"title":"Hematological alterations in Egyptian farmers occupationally exposed to pesticides","authors":"Hany Desouky, N. Rizk, Huda Said, Reham Abd El-Azeem","doi":"10.21608/jmals.2023.307496","DOIUrl":"https://doi.org/10.21608/jmals.2023.307496","url":null,"abstract":"Background: Pesticides are agrochemicals extensively used all over the world for pest control and prevention, Egyptian farmers are occupationally exposed to many types of pesticides, making them at high risk for pesticide toxicity. Several studies have shown acute pesticide poisoning effects, but fewer have dealt with chronic pesticide toxicity, especially studies regarding pesticide's effects on hematological parameters. Objective: We aimed to evaluate hematological parameter changes related to long-term low-level pesticide exposure. Subjects and Methods: The blood samples were collected from 96 Egyptian farmers chronically exposed to mixed pesticides and 54 nonexposed controls. A Complete Blood Picture (CBC) was evaluated to explore the hematological indices alterations. Statistical analysis was conducted using IBM SPSS Statistics, version 25. Results: A significant decrease in hemoglobin, hematocrit, mean corpuscular volume, and mean corpuscular hemoglobin in addition to platelet count in the exposed farmers compared to the control, On the other hand, white blood cell count was significantly increased. Conclusion: Chronic occupational pesticide exposure of Egyptian farmers leads to a range of disorders in hematological indices. As an affordable and readily available test, the Complete Blood Picture can help in the chronic monitoring of farmers health status who are regularly exposed to pesticides.","PeriodicalId":406966,"journal":{"name":"Journal of Medical and Life Science","volume":"100 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-07-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"129258535","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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