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Allelopathic Impact of Callistemon Citrinus on Sorghum Bicolor Growth under Salinity Stress Callistemon Citrinus 在盐度胁迫下对双色高粱生长的异化作用
Pub Date : 2024-05-17 DOI: 10.9734/ajrcs/2024/v9i2269
Hamed, Badr eldin Abd ElAal, Sayed, Mona
The current study assessed the impact of 0.09% aqueous leaf extract from Callistemon citrinus on the growth of two cultivars of Sorghum bicolor L. (Giza 15 - G15 and Dorado - Dor) under two salinity levels (100 mM and 200 mM NaCl). Results revealed that salinity reduced various growth criteria, including insoluble and total carbohydrates, photosynthetic pigments, protein, total phenols, and reduced glutathione, while soluble carbohydrates (sucrose and trehalose) were influenced. Salinity also affected free amino acids (proline and glycine betaine), malondialdehyde, and ascorbic acid. The activity of amylase decreased, while antioxidant enzymes (catalase, peroxidase, superoxide dismutase, ascorbic acid oxidase, and glutathione peroxidase) increased. Additionally, concentrations of K+, Ca++, and Mg++ were reduced, while Na+ accumulated. Application of Callistemon extract improved plant growth under stressed or normal growth conditions. The aqueous leaf extract (0.09%) of Callissstemon sp., with its enriched phenolic allelochemicals, mitigated the impact of salinity by enhancing photosynthesis, osmoregulation, and the antioxidant defense system of sorghum plants. Furthermore, results indicated that Sorghum cultivar G15 exhibited more resistance than Dor, with this resistance correlating with the activity of the antioxidant system.
本研究评估了在两种盐度水平(100 mM 和 200 mM NaCl)下,0.09% 的柠檬茧叶水提取物对两种高粱品种(Giza 15 - G15 和 Dorado - Dor)生长的影响。结果表明,盐度降低了各种生长标准,包括不溶性碳水化合物和总碳水化合物、光合色素、蛋白质、总酚和还原型谷胱甘肽,而可溶性碳水化合物(蔗糖和三卤糖糖)则受到影响。盐度还影响游离氨基酸(脯氨酸和甘氨酸甜菜碱)、丙二醛和抗坏血酸。淀粉酶的活性下降,而抗氧化酶(过氧化氢酶、过氧化物酶、超氧化物歧化酶、抗坏血酸氧化酶和谷胱甘肽过氧化物酶)的活性上升。此外,K++、Ca++ 和 Mg++ 的浓度降低,而 Na+ 的浓度增加。在胁迫或正常生长条件下,施用 Callistemon 提取物都能改善植物的生长。Callissstemon sp.的叶片水提取物(0.09%)含有丰富的酚类等位化学物质,能增强高粱植物的光合作用、渗透调节和抗氧化防御系统,从而减轻盐碱的影响。此外,研究结果表明,高粱栽培品种 G15 比 Dor 表现出更强的抗性,这种抗性与抗氧化系统的活性有关。
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引用次数: 0
Phytotoxicity and Residual Effect of Some Herbicides on three Egyptian Rice Cultivars (Oryza sativa L.) 某些除草剂对三种埃及水稻品种(Oryza sativa L.)的植物毒性和残留效应
Pub Date : 2024-05-16 DOI: 10.9734/ajrcs/2024/v9i2268
.. Abd El-Naby, S. S. M., A. El-Ghandor, E. A. A. Marzoka, H. M. Soliman
The current study was carried out at the experimental farm of Rice Research Department, Sakha Agricultural Research Station and Central Agricultural Pesticide Laboratory, ARC, Egypt during the summer season of 2021 and 2022 to study the sensitivity of three Egyptian rice cultivars against four common herbicides and its effect on physiological and agronomic characteristics of rice as well as herbicide residues in rice seeds. The field study was laid out in split plot design with three replications. Three rice cultivars (Giza 177, Giza 179 and Sakha 108) were randomly distributed in main plots, while weed control treatments included four herbicides at recommended doses (penoxsulam 2.5% OD, bispyribac-sodium 2% SL, cyhalofop-butyl 10% EC and fenoxaprop-p-ethyl 7.5% EW) as compared to hand weeding were devoted in sub plots. The obtained results revealed that Sakha 108 as Japonica rice cultivar was resistant against tested herbicides and recorded the highest values of rice dry weight, chlorophyll content, number of panicles m-2, panicle weight and grain yield, while scored the lowest values of total phenols. On the other hand, Giza 177 as short duration Japonica rice cultivar showed the higher sensitivity against tested herbicides and achieved the lowest values of abovementioned agronomic traits and chlorophyll content. Cyhalofop-butyl as ACCase herbicide was safer on studied rice cultivars and recorded the highest values of studied agronomic characteristics for rice as well as chlorophyll content, while recorded the lowest values of total phenols during the study. On the other hand, bispyribac-sodium 2% SL resulted in more toxic effect for rice cultivars and recorded the lowest values of studied agronomic traits for rice. fenoxaprop-p-ethyl 7.5% EW achieved the lowest chlorophyll content and highest values of total phenols during the study. Giza 179 as Indica-Japonica rice cultivar appeared high sensitivity when sprayed with bispyribac-sodium and fenoxaprop-ethyl but made recovery after 12 days from herbicidal application and recorded the highest chlorophyll content, dry weight, panicles m-2 and grain yield with no significant differences between Sakha 108 rice cultivar during the study. In respect of herbicide residues in rice seeds, all tested herbicides didn’t have residues in rice seeds at 100 days after treatment. It means that tested herbicides didn’t have any residues in rice seeds. 
本研究于 2021 年和 2022 年夏季在埃及萨哈农业研究站水稻研究部实验农场和 ARC 中央农业杀虫剂实验室进行,旨在研究埃及三种水稻栽培品种对四种常见除草剂的敏感性及其对水稻生理和农艺特性以及水稻种子中除草剂残留的影响。田间研究采用分小区设计,三次重复。三种水稻栽培品种(吉萨 177、吉萨 179 和 Sakha 108)随机分布在主小区,而除草处理包括四种除草剂(五氟磺草胺 2.5% OD、双唑钠 2% SL、氰氟草酯 10% EC 和精噁唑禾草灵 7.5% EW),与人工除草进行比较。结果表明,粳稻品种 Sakha 108 对测试的除草剂具有抗性,其水稻干重、叶绿素含量、圆锥花序数 m-2、圆锥花序重和谷物产量的值最高,而总酚的值最低。另一方面,Giza 177 作为短期粳稻栽培品种,对测试的除草剂表现出较高的敏感性,上述农艺性状和叶绿素含量的值最低。氰氟草酯作为 ACCase 除草剂,对所研究的水稻品种更安全,在研究期间记录到的水稻农艺特征值和叶绿素含量最高,而总酚值最低。另一方面,2% SL 的双草醚对水稻栽培品种的毒性作用更大,水稻农艺性状的研究值也最低。Giza 179 作为籼粳稻栽培品种,在喷洒双草醚和精噁唑禾草灵后出现高敏感性,但在施用除草剂 12 天后恢复,叶绿素含量、干重、圆锥花序 m-2 和谷物产量最高,与 Sakha 108 水稻栽培品种在研究期间没有显著差异。关于除草剂在水稻种子中的残留,所有测试的除草剂在施药 100 天后都没有在水稻种子中残留。这说明受试除草剂在水稻种子中没有任何残留。
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引用次数: 0
BRRI Dhan107: High Protein Premium Quality Rice Variety for Irrigated Ecosystem in Bangladesh BRRI Dhan107:孟加拉国灌溉生态系统的高蛋白优质水稻品种
Pub Date : 2024-05-13 DOI: 10.9734/ajrcs/2024/v9i2267
Md. Abdul Kader, R. Majumder, T. Hore, Urmi Rani Shaha, A. Shalahuddin
A newly released high protein enriched, premium quality type high yielding, extra-long slender grain containing rice variety BRRI dhan107 which suitable for irrigated ecosystem of Bangladesh, is an improvement over existing high protein enriched and premium quality type rice varieties for Bangladesh. The variety has satisfactorily passed the Proposed Variety Trial (PVT) conducted in the farmers’ field in Boro 2022-23 season. As a result National Seed Board (NSB) approved this variety for commercial cultivation in the Boro season (dry season) in 2024. It has modern plant type with 103 cm plant height and matures by 148 days. The proposed variety showed 1.20 t/ha higher yield than check variety BRRI dhan50. Identifying characters of this variety are green leaf, intermediate plant height, semi-erect flag leaf, extra-long slender grain, intermediate leaf senescence. On an average it can produce 8.19 t/ha grain yield, although with appropriate management under favorable environment it can give up to 9.57 t/ha yield. Thousand grain weight of the variety is 26.1 gm and head rice yield is 60.1%. High protein (10.02%) enriched BRRI dhan107 has high amylose (29.1%) with extra-long slender (7.6 mm) type grain. The high protein enriched, premium quality type rice variety BRRI dhan107 is a superb variety for cultivating in the Boro season (dry) and farmers as well as the country can be nutritionally and economically benefited by the cultivation of BRRI dhan107.
新推出的高蛋白、优质型高产特细长粒水稻品种 BRRI dhan107 适合孟加拉国的灌溉生态系统,是对孟加拉国现有的高蛋白、优质型水稻品种的改进。该品种顺利通过了 2022-23 年度在农民田间进行的拟议品种试验(PVT)。因此,国家种子委员会(NSB)批准该品种在 2024 年波罗季(旱季)进行商业种植。该品种为现代株型,株高 103 厘米,成熟期 148 天。该品种的产量比对照品种 BRRI dhan50 高 1.20 吨/公顷。该品种的识别特征为绿叶、中等株高、半直立旗叶、超长细粒、中等叶片衰老。平均产量为 8.19 吨/公顷,但如果在有利环境下进行适当管理,产量可达 9.57 吨/公顷。该品种的千粒重为 26.1 克,头米率为 60.1%。富含高蛋白(10.02%)的 BRRI dhan107 具有高直链淀粉(29.1%)和超长细粒(7.6 毫米)。富含高蛋白的优质水稻品种 BRRI dhan107 是一个非常适合在旱季种植的优良品种,农民和国家都能从 BRRI dhan107 的种植中获得营养和经济效益。
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引用次数: 0
Yield Maximization of Rice (Oryza sativa) through Integrated Nutrient Management 通过综合养分管理实现水稻(Oryza sativa)产量最大化
Pub Date : 2024-05-10 DOI: 10.9734/ajrcs/2024/v9i2266
Md. Mamunur Rashid, Setara Begum, Mohammad Rezaul Manir, Md. Sirajul Islam, A. Shalahuddin, Afsana Jahan, Md. Mahamudul Hasan Musha, Md. Arefin Hasan
This experiment was conducted on a permanent layout at the west byde of BRRI farm, Gazipur during T. Aman 2019 to Boro 2021-22 to find out the suitable management practices for yield maximization of rice and soil health. Seven treatments in Randomized Complete Block (RCB) Design with three replications were imposed and each treatment was assigned in 5m × 4m sized plot. The treatments combinations were T1= Absolute Control (No nutrient supply), T2 = BRRI dose N-P-K-S@83-17-53-12 kg ha-1 in T. Aman and 138-21-75-21 kg ha-1 in Boro season, T3= Soil Test Based (STB) Fertilizer Dose N-P-K-S @ 67-10-40-10 kg ha-1 in T. Aman and 134-16-75-10 kg ha-1 in Boro, T4 = STB dose + 1 t ha-1 Cowdung, T5 = STB dose + 1 t ha-1 Poultry manure, T6 = STB dose + 1 t ha-1 Vermicompost and T7 = STB dose + 0.33 t ha-1 CD + 0.33 t ha-1 PM + 0.33 t ha-1 VC. Thirty-days old seedling of BRRI dhan87 in T. Aman and fourty-two days old seedling of BRRI dhan89 in Boro season were transplanted at 20 cm × 20 cm spacing in both seasons. All manures, soil and plant samples analysis were done by the help of soil Science Division BRRI, Gazipur. Initial soil (0-15 cm depth) properties were: soil texture, clay loam; pH, 6.94; organic Carbon, 1.59%; Nitrogen, 0.18%; Phosphorus, 21.88 ppm and Potassium, 0.19meq/100g soil. Thirty days old seedling of BRRI dhan87 in T. Aman and 42 days old seedling of BRRI dhan89 in Boro season were transplanted at 20cm x 20cm spacing. Grain yield, tiller number, panicle number, plant height and grain number were significantly affected by the different integrated nutrient management during both T. Aman and Boro season. Grain yield, tiller number, panicle number, plant height and grain number were significantly affected by the different nutrient management in both T. Aman and Boro season. Poultry manure related treatments and BRRI recommended dose performed better than the others in all the parameter except 1000-grain weight. On the other hand, Absolute Control (No nutrient supply) produced the lowest result.  Every parameter, Poultry manure treatments have been performed the best. STB dose with one t ha-1 poultry manure is better for maximization of rice yield.
该试验于 2019 年天安节至 2021-22 年勃朗节期间在加济布尔 BRRI 农场西侧的永久性布局上进行,旨在找出实现水稻产量最大化和土壤健康的合适管理方法。采用随机完全区组(RCB)设计,共设七个处理,三个重复,每个处理分配在 5m × 4m 大小的地块中。处理组合为:T1= 绝对对照(无养分供应);T2= BRRI 剂量 N-P-K-S@83-17-53-12 kg ha-1(天安季节)和 138-21-75-21 kg ha-1(波罗季节);T3= 土壤测试(STB)肥料剂量 N-P-K-S@67-10-40-10 kg ha-1(天安季节)和 134-16-75-21 kg ha-1(波罗季节)。T4 = STB 剂量 + 1 吨/公顷-1 牛粪,T5 = STB 剂量 + 1 吨/公顷-1 禽粪,T6 = STB 剂量 + 1 吨/公顷-1 蛭石,T7 = STB 剂量 + 0.33 吨/公顷-1 CD + 0.33 吨/公顷-1 PM + 0.33 吨/公顷-1 VC。两季均以 20 厘米 × 20 厘米的株行距移栽了天安时 30 天大的 BRRI dhan87 幼苗和波罗时 42 天大的 BRRI dhan89 幼苗。所有肥料、土壤和植物样本的分析都是在加济布尔 BRRI 土壤科学部的帮助下完成的。初始土壤(0-15 厘米深)特性为:土壤质地为粘壤土;pH 值为 6.94;有机碳含量为 1.59%;氮含量为 0.18%;磷含量为 21.88 ppm;钾含量为 0.19meq/100g 土壤。以 20 厘米 x 20 厘米的株行距移栽了天安季节 30 天大的 BRRI dhan87 秧苗和波罗季节 42 天大的 BRRI dhan89 秧苗。安缦季和波罗季的谷物产量、分蘖数、圆锥花序数、株高和粒数均受到不同综合养分管理的显著影响。安缦季和波罗季的谷物产量、分蘖数、圆锥花序数、株高和谷粒数受不同养分管理的显著影响。除千粒重外,家禽粪便相关处理和 BRRI 推荐剂量在所有参数上的表现均优于其他处理。另一方面,绝对对照(不供应养分)的结果最低。 在所有参数中,家禽粪肥处理的表现最好。添加 1 吨/公顷家禽粪便的 STB 剂量能更好地提高水稻产量。
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引用次数: 1
Comparative Performance of Different Fertilizer Recommendation Methods on Growth and Yield of Rice (Oryza sativa) in Old Brahmaputra Floodplain Soils 不同施肥方法对雅鲁藏布江冲积平原老土中水稻生长和产量的影响比较
Pub Date : 2024-04-15 DOI: 10.9734/ajrcs/2024/v9i2264
Shadia Akter, Md Hosenuzzaman, M. Abedin, Md Anamul Hoque
Improving rice productivity is the challenge for the farmer due to rapid soil health deterioration in intensive agricultural system. Therefore, the present study was conducted to evaluate comparative performance of different nutrient management practices in rice production in Bangladesh. The experiment was laid out in a randomized complete block design with three replications. There      were six treatments namely, T1 (Control-no fertilizer), T2 (Farmer’s Practice), T3 (Fertilizer Recommendation Guide-2018), T4 (BAU Soil Testing Kit), T5 (Soil Test Basis) and T6 (Rice Crop Manager). The results revealed that all the treatments showed better performances over control (T1). Treatment T4 (BAU Soil Testing Kit) produced the highest value of plant height (90.18 cm), panicle length (24.90), filled grains panicle-1 (122.60) and 1000-grain weight (26.67g) while T5 (Soil Test Basis) produced maximum effective tillers hill-1 (12.80). The highest grain yield (6.29 t ha-1) and straw yield (7.73 t ha-1) were recorded in treatment T4 (BAU Soil Testing Kit). Treatment T4 (BAU Soil Testing Kit) also showed the highest grain yield increase (61.49%) and straw yield increase (90.49%) over control. The highest total N, P and K uptake (108.84 kg ha-1, 24.54 kg ha-1 and 128.17 kg ha-1) were obtained in the treatment T4 (BAU Soil Testing Kit). Control treatment T1 (No fertilizers) showed minimum performances in all the cases compared to other treatments. The overall result demonstrated that the application of fertilizers following BAU Soil Testing Kit method of fertilizer application system is a better option for rice production.
在集约化农业系统中,由于土壤健康状况迅速恶化,提高水稻产量对农民来说是一项挑战。因此,本研究对孟加拉国水稻生产中不同养分管理方法的性能进行了比较评估。实验采用随机完全区组设计,三次重复。共有六个处理,即 T1(对照-不施肥)、T2(农民实践)、T3(肥料推荐指南-2018)、T4(BAU 土壤测试工具包)、T5(土壤测试基础)和 T6(水稻作物管理器)。结果表明,与对照组(T1)相比,所有处理都有更好的表现。处理 T4(BAU 土壤测试工具包)的株高(90.18 厘米)、穗轴长(24.90)、穗轴粒数(122.60)和千粒重(26.67 克)最高,而处理 T5(土壤测试基础)的有效分蘖数(12.80)最高。处理 T4(BAU 土壤测试套件)的谷物产量(6.29 吨/公顷-1)和稻草产量(7.73 吨/公顷-1)最高。与对照相比,处理 T4(BAU 土壤检测试剂盒)的谷物产量增幅(61.49%)和秸秆产量增幅(90.49%)也最高。处理 T4(BAU 土壤测试工具包)的氮、磷、钾总吸收量最高(108.84 千克/公顷-1、24.54 千克/公顷-1 和 128.17 千克/公顷-1)。与其他处理相比,对照处理 T1(不施肥)在所有情况下的表现都最低。总体结果表明,采用 BAU 测土配方施肥系统施肥是水稻生产的较佳选择。
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引用次数: 0
Esterified Conditioner and Repairing Agent for Salinity Affected Agricultural Soil of India 印度受盐碱影响农田土壤的酯化调节剂和修复剂
Pub Date : 2024-04-12 DOI: 10.9734/ajrcs/2024/v9i2263
S. Chaudhary, Ratna Trivedi, Jay Bergi
Various bio-conversion reactions given by lipases such as hydrolysis, transesterification, esterification, alcohoysis, acidolysis and aminolysis. The potential esterification process in soil plays important role not only as a fertilizer or nutrient but also act as a modulator to alter the conditions of soil and help in uptake of nutrient and minerals. This potential formulation accumulates micro-nutrients and mineral that promotes growth of plants. The trials of vegetable plants and grains shown significant increase in crop yield per hector area.  The bulk density and field capacity of the soil are also significantly change and act as soil conditioner also influence the growth of plants. The micronutrients uptake of plant helps optimize the soil pH and improve the soil conditions. The inventors of present invention tested formulation as plant growth promoters that enhance crop characteristics like panicle length, plant height, grain or fruit yield and add to nutrient value of the crop. Our product is fatty acid composite which play crucial role as plant growth promoter along with reducing seed germination time and increasing plant crop yield, it also beneficial to work under water stress conditions and variant geo-climatic conditions.
脂肪酶可进行各种生物转化反应,如水解、酯交换、酯化、醇解、酸解和氨解。土壤中的潜在酯化过程不仅是一种重要的肥料或营养物质,而且还是改变土壤条件的调节剂,有助于吸收养分和矿物质。这种潜在配方可积累微量营养元素和矿物质,促进植物生长。对蔬菜植物和谷物的试验表明,单位面积作物产量显著增加。 土壤的容重和田间容量也发生了显著变化,作为土壤改良剂也影响了植物的生长。植物对微量营养元素的吸收有助于优化土壤 pH 值和改善土壤条件。本发明的发明者测试了作为植物生长促进剂的配方,该配方可提高作物特性,如圆锥花序长度、株高、谷物或果实产量,并增加作物的营养价值。我们的产品是脂肪酸复合制剂,作为植物生长促进剂,它不仅能缩短种子发芽时间,提高作物产量,还能在水胁迫条件和不同的地理气候条件下发挥作用。
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引用次数: 0
Effect of Bacillus pumilus Strains on Heavy Metal Accumulation in Lettuce Grown on Contaminated Soil 枯草芽孢杆菌菌株对污染土壤中种植的生菜重金属积累的影响
Pub Date : 2024-04-10 DOI: 10.9734/ajrcs/2024/v9i2262
Sabeen Alam, Mehboob Alam, Hafsa Naeem, Muhammad Adeel, Adil Riaz, Usama Zeb Awan, Abdul Basit, Burhan Naeem
Increasing number of heavy metal on land needs to be addressed through sustainable ways and various species of Bacillus can be used to mitigate heavy metals. The research work entitled “Effect of Bacillus pumilus strains on heavy metal accumulation in lettuce grown on contaminated soil” focuses on functional role of Bacillus pumilus strains inoculated with lettuce seed in mitigating heavy metal present in chromite mining soil. This experiment was conducted at ornamental horticulture nursery, the University of Agriculture Peshawar. In this experiment, factor A was three Bacillus pumilus strains (sequence C-2PMW-8, C-1 SSK-8 and C-1 PWK-7) while soil used for this experiment was collected from Prang Ghar mining site and lettuce seeds were grown in three levels of chromite mining soil (2.27, 4.65 and 7.14 %).The experimental design used during this research was randomized complete block design with two factors and was replicated thrice. The collected data related to effect of chromite mining soil and Bacillus pumilus strains indicated that these both factors have significant influence on growth parameters. For mining soil minimum days to germinate (14 days) was noted in lettuce grown on garden soil inoculated with sequence. Maximum germination percentage noted was (100%) for C-1 SSK-8 grown on garden soil, dry weight of lettuce leaf (5.0 g) for lettuce inoculated with C-1 SSK-8 and C-1 PWK-7 strains, survival percentage (77.9%) for C-1 SSK-8 treated lettuce for sequence C-2 PMW-8. Maximum germination percentage noted was (88%) for garden soil, fresh leaf weight (17.3g), dry leaf weight (6.5g), number of leaf per plant (16), leaf area (49.0 cm2) were noted in garden soil with no chromite mining soil. Results related to heavy metals accumulation showed that minimum chromium was (2.3 mg kg-1) in lettuce and (1.9 mg kg-1) in soil for all three sequences. It can be concluded that chromite mining soil significantly reduced the growth and survival of lettuce, but when lettuce was inoculated with Bacillus.pumilus strains it enhances the growth and survival. Similarly, minimum heavy metal accumulation in plant and soil, regardless of type of B. pumilus used, all three sequences has same mitigating effect on heavy metal in both soil and lettuce. All the three Bacillus pumilus strains ensured reduction in heavy metals content (Cr) in lettuce, below the maximum permissible limits of WHO/FAO 2011.
土地上的重金属越来越多,需要通过可持续的方式来解决,而各种芽孢杆菌可用于缓解重金属。这项题为 "枯草芽孢杆菌菌株对污染土壤上种植的莴苣重金属积累的影响 "的研究工作,重点研究了接种莴苣种子的枯草芽孢杆菌菌株在减轻铬铁矿土壤中重金属含量方面的功能作用。该实验在白沙瓦农业大学观赏园艺苗圃进行。在该实验中,因素 A 是三种枯草芽孢杆菌菌株(序列 C-2PMW-8、C-1 SSK-8 和 C-1 PWK-7),而实验所用的土壤是从 Prang Ghar 矿区采集的,莴苣种子生长在三种含量的铬铁矿土壤中(2.27%、4.65% 和 7.14%)。收集到的有关铬铁矿土壤和枯草芽孢杆菌菌株影响的数据表明,这两个因素对生长参数有显著影响。在矿区土壤中,接种了该菌株的莴苣发芽天数最少(14 天)。接种 C-1 SSK-8 和 C-1 PWK-7 菌株的莴苣叶片干重(5.0 克),C-1 SSK-8 处理莴苣的存活率(77.9%),C-2 PMW-8 处理莴苣的存活率(77.9%)。花园土壤的发芽率最高(88%),鲜叶重量(17.3 克)、干叶重量(6.5 克)、单株叶片数(16)、叶面积(49.0 平方厘米)均高于无铬铁矿土壤。与重金属积累有关的结果显示,在所有三个序列中,生菜中的铬含量最低(2.3 毫克/千克-1),土壤中的铬含量最低(1.9 毫克/千克-1)。由此可以得出结论,铬铁矿土壤明显降低了莴苣的生长和存活率,但当莴苣接种枯草芽孢杆菌菌株后,其生长和存活率均有所提高。同样,无论使用哪种枯草芽孢杆菌,植物和土壤中的重金属累积量都最小,所有三种菌序对土壤和莴苣中的重金属都有相同的缓解作用。所有三种枯草芽孢杆菌菌株都能确保降低莴苣中的重金属(Cr)含量,使其低于世界卫生组织/粮农组织 2011 年规定的最高允许限值。
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引用次数: 0
Improving of Amaranth (Amaranthus Spp.) and Quinoa (Chenopodium quinoa) by Genetic Resources 利用遗传资源改良苋菜(Amaranthus Spp.)和藜麦(Chenopodium quinoa)
Pub Date : 2024-04-06 DOI: 10.9734/ajrcs/2024/v9i2261
I. Rehman, Narmeen Ayesha, Khadija Anam, A. Khalid, Laraib Ali, Hina Nazir, Zabeehullah Burhan, Usama Sadique
The modern human population is more mindful of their diet and choose foods carefully in order to maintain a healthy lifestyle and prevent illness. Thus, instead of sticking to their long-standing diets of ordinary cereals and basic foods, individuals are starting to choose more intelligent and nutrient-dense dietary choices. Because they are gluten-free, have no added sugar, and are somewhat higher in nutrients than typical cereals, pseudocereals—particularly quinoa and amaranth—are significant substitutes. Both Amaranthaceae crops are hardy, low-input plants that can withstand salt, stress, and drought. Therefore, these crops might be advantageous to emerging nations with limited agricultural resources and subsistence agriculture. But these are neglected orphan crops, and for a very long time, there has been no attempt to enhance them by lowering their saponin content. These crops also have a great degree of variety, but their genetic development towards high-yielding genotypes is sluggish. This is because traditional cereals are facing problems, and crop diversification is the preferred solution to address climate change. The most recent technological advancements that can speed up breeding to increase agricultural output and quality are far behind and move more slowly than the world's primary crops that are already well-established.
现代人更加注重饮食,为了保持健康的生活方式和预防疾病,他们会谨慎选择食物。因此,人们不再固守长期以来的普通谷物和基本食物的饮食习惯,而是开始选择更明智、营养更丰富的饮食。伪谷物,尤其是藜麦和苋菜,由于不含麸质、不添加糖分,而且营养成分略高于普通谷物,因此是重要的替代品。这两种苋科作物都是耐寒、低投入的植物,能够抵御盐、压力和干旱。因此,这些作物对于农业资源有限、农业仅能维持生存的新兴国家来说可能是有利的。但是,这些作物是被忽视的 "孤儿作物",长期以来,人们一直没有尝试通过降低皂苷含量来提高它们的产量。这些作物种类繁多,但向高产基因型的遗传发展却十分缓慢。这是因为传统谷物面临着各种问题,而作物多样化是应对气候变化的首选方案。与世界上已经成熟的主要作物相比,能够加快育种以提高农业产量和质量的最新技术进步还远远落后,发展速度也更慢。
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引用次数: 0
Growth and Production Stimulation of Young Pink Oyster Mushrooms (Pleurotus flabellatus) through CaCO3 Supplementation from Shellfish Shells and Coconut Water 通过贝类贝壳和椰子水补充 CaCO3 刺激粉红杏鲍菇(Pleurotus flabellatus)幼菌的生长和生产
Pub Date : 2024-04-05 DOI: 10.9734/ajrcs/2024/v9i1260
Lili Yamurni, Julia Marisa, Ruth Riah Ate Tarigan
Pink oyster mushroom (Pleurotus flabellatus) is one of the oyster mushroom varieties that has a distinctive aroma similar to lingzhi mushroom, pink color, numerous clusters, and a slightly firm texture. The aim of this research was to determine the growth and production stimulation of pink oyster mushrooms (P. flabellatus) through supplementation of CaCO3 from shellfish shells and coconut water. This study utilized a Completely Randomized Design (CRD) arranged in factorial with two factors, consisting of four levels with three replications. The first factor was CaCO3 supplementation from shellfish shells with four levels: K0: 0 gr/baglog, K1: 5 gr CaCO3 from shellfish shells/baglog, K2: 10 gr CaCO3 from shellfish shells/baglog, K3: 15 gr CaCO3 from shellfish shells/baglog. The second factor was coconut water supplementation with four levels: A0: 0 ml/baglog, A1: 20 ml coconut water/baglog, A2: 40 ml coconut water/baglog, A3: 60 ml coconut water/baglog. The results showed significant differences in mycelium growth rate and highly significant differences in pileus number, pileus width, pileus thickness, stalk height, and mushroom fresh weight. Supplementation of 10 gr CaCO3 from shellfish shells/baglog and 40 ml coconut water/baglog showed the best interaction for all parameters.
粉红杏鲍菇(Pleurotus flabellatus)是杏鲍菇品种之一,具有类似灵芝的独特香气,色泽粉红,菌团众多,质地稍硬。本研究旨在确定通过补充贝壳和椰子水中的 CaCO3 对粉红杏鲍菇(P. flabellatus)生长和产量的刺激作用。本研究采用了完全随机设计(CRD),包含两个因子,四个水平,三次重复。第一个因子是贝壳中 CaCO3 的补充,有四个水平:K0: 0 gr/baglog, K1: 5 gr CaCO3 from shellfish shells/baglog, K2: 10 gr CaCO3 from shellfish shells/baglog, K3: 15 gr CaCO3 from shellfish shells/baglog.第二个因素是椰子水的补充,有四个水平:A0:0 毫升/袋;A1:20 毫升/袋;A2:40 毫升/袋;A3:60 毫升/袋。结果表明,菌丝生长速度存在显著差异,菌褶数量、菌褶宽度、菌褶厚度、菌柄高度和蘑菇鲜重存在高度显著差异。添加 10 克贝壳中的 CaCO3/袋液和 40 毫升椰子汁/袋液对所有参数的交互作用最好。
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引用次数: 0
Analysis of Genetic Variability of Potato Induced Mutants Using Simple Sequence Repeat (SSR) Markers 利用简单序列重复(SSR)标记分析马铃薯诱导突变体的遗传变异性
Pub Date : 2024-03-30 DOI: 10.9734/ajrcs/2024/v9i1259
E. Chepkoech, M. Kinyua, Oliver Kiplagat, Julius Ochuodho, S. Bado, S. Kimno
Aims: Potato (Solanum tuberosum L.) is ranked fourth amongst the world’s major stable food crop and second in Kenya after maize and thus plays a vital role in food and nutrition security and sustainable development. Despite its importance, potato production in Kenya is still low due to abiotic and biotic constraints. Traditional breeding and improvement have been difficult due to the narrow genetic diversity of the crop owed to the complex tetrasomic inheritance patterns. Induced mutation has been used to generate genetic variations in potato from which desired putative mutants are selected. In most cases the level of genetic variability is not known. The objective of this study was to determine the genetic variability of potato mutants using SSR markers.Study Design: The study involved three potato varieties; Asante, Kenya Mpya and Kenya Sherekea which are high yielding and commercially grown varieties in Kenya.Place and Duration of Study: Sample: They were irradiated at different dose rates of gamma rays at Co60 source at FAO/IAEA laboratories, Seiberdorf Austria in 2014. A total of 163 mutants were advanced to M1V4 generation at the University of Eldoret between 2015 and 2018.Methodology: Genomic DNA was extracted to assess the diversity with 20 SSRs markers.Results: All the 20 SSR primers were polymorphic with 6-19 bands amplified per primer and marker STM5127 showed the highest allele number (19) using PowerMarker software. The STRUCTURE analysis suggested that the potato mutants were clustered into six sub-populations based on the unweighted pair-group method of arithmetic averages (UPGMA) thus, the accessions were divided into three major clusters. Analysis of molecular variance (AMOVA) indicated that 8.6% of total molecular variance was attributed to diversity among sub-populations, while 91.4% of variance was associated with differences within sub-populations.Conclusion: This study highlights the most comprehensive investigation of the genetic diversity and population structure of potato mutants, and provides valuable information for genetic improvement, and systematic utilization.
目的:马铃薯(Solanum tuberosum L.)在世界主要稳定粮食作物中排名第四,在肯尼亚仅次于玉米,因此在粮食和营养安全以及可持续发展方面发挥着至关重要的作用。尽管马铃薯十分重要,但由于受到非生物和生物因素的制约,肯尼亚的马铃薯产量仍然很低。由于复杂的四体遗传模式导致作物遗传多样性狭窄,传统的育种和改良十分困难。诱导突变已被用于产生马铃薯的遗传变异,从中筛选出所需的假定突变体。在大多数情况下,遗传变异的水平并不清楚。本研究的目的是利用 SSR 标记确定马铃薯突变体的遗传变异性:研究涉及三个马铃薯品种:Asante、Kenya Mpya 和 Kenya Sherekea,它们都是肯尼亚的高产和商业化种植品种:样品:2014年在奥地利塞伯多夫的粮农组织/国际原子能机构实验室用Co60源以不同剂量率的伽马射线对其进行辐照。2015年至2018年期间,共有163个突变体在埃尔多雷特大学进化到M1V4代:提取基因组 DNA,用 20 个 SSR 标记评估多样性:使用 PowerMarker 软件,所有 20 个 SSR 引物均具有多态性,每个引物可扩增 6-19 条带,标记 STM5127 显示出最高等位基因数(19)。STRUCTURE 分析表明,根据非加权对组算术平均法(UPGMA),马铃薯突变体被聚类为六个亚群,因此,加入的马铃薯突变体被分为三大群。分子方差分析(AMOVA)表明,分子总方差的 8.6%归因于亚群之间的多样性,而 91.4%的方差与亚群内部的差异有关:本研究是对马铃薯突变体遗传多样性和种群结构最全面的调查,为遗传改良和系统利用提供了宝贵的信息。
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引用次数: 0
期刊
Asian Journal of Research in Crop Science
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