Pub Date : 2022-11-03DOI: 10.29235/1561-8323-2022-66-5-501-508
I. E. Glazov, V. Krut’ko, O. Musskaya, A. Kulak
Amorphous calcium carbonate-phosphate with a Ca/P ratio of 1.83 was precipitated from Ca2+, Ca2+, PO3–4, CO2-3 − containing solutions at pH 10 and stabilized by ethanol dehydration and followed by heating at 400 °C. The presence of PO3–4 ions in the structure of amorphous calcium carbonate-phosphate provides its increased resistance to transformation into crystalline phases. Aging in a Ca2+, PO3–4 , CO2-3 − containing mother solution at pH 10 for 4 days promotes the transformation of amorphous calcium carbonate-phosphate into amorphous calcium carbonate-phosphate / carbonated hydroxyapatite / calcite. The combined effect of PO3–4 и CO2-3 − ions in the aquatic environment on the crystallization of amorphous calcium carbonate-phosphate into carbonated hydroxyapatite contributes to a maximum stabilization of the amorphous state that provides a high extent of bioactivity.
{"title":"Stabilization of the amorphous state of calcium carbonate-phosphates with phosphate ions","authors":"I. E. Glazov, V. Krut’ko, O. Musskaya, A. Kulak","doi":"10.29235/1561-8323-2022-66-5-501-508","DOIUrl":"https://doi.org/10.29235/1561-8323-2022-66-5-501-508","url":null,"abstract":"Amorphous calcium carbonate-phosphate with a Ca/P ratio of 1.83 was precipitated from Ca2+, Ca2+, PO3–4, CO2-3 − containing solutions at pH 10 and stabilized by ethanol dehydration and followed by heating at 400 °C. The presence of PO3–4 ions in the structure of amorphous calcium carbonate-phosphate provides its increased resistance to transformation into crystalline phases. Aging in a Ca2+, PO3–4 , CO2-3 − containing mother solution at pH 10 for 4 days promotes the transformation of amorphous calcium carbonate-phosphate into amorphous calcium carbonate-phosphate / carbonated hydroxyapatite / calcite. The combined effect of PO3–4 и CO2-3 − ions in the aquatic environment on the crystallization of amorphous calcium carbonate-phosphate into carbonated hydroxyapatite contributes to a maximum stabilization of the amorphous state that provides a high extent of bioactivity.","PeriodicalId":41825,"journal":{"name":"DOKLADY NATSIONALNOI AKADEMII NAUK BELARUSI","volume":null,"pages":null},"PeriodicalIF":0.1,"publicationDate":"2022-11-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46621881","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-11-02DOI: 10.29235/1561-8323-2022-66-5-495-500
D. Novitsky, S. Gaponenko
A possible effect of the low photon density of states in plasma on the Lamb shift is analysed. It is found that because of a significant contribution of high-energy virtual photons to the Lamb shift, its modification in plasma does not exceed 1 % with respect to vacuum even at electron concentrations as high as 1022 cm–3. This behavior results from an asymptotic tendency of plasma properties to vacuum ones at an unlimited frequency growth.
{"title":"Photon density of states effect on Lamb shift in plasmas","authors":"D. Novitsky, S. Gaponenko","doi":"10.29235/1561-8323-2022-66-5-495-500","DOIUrl":"https://doi.org/10.29235/1561-8323-2022-66-5-495-500","url":null,"abstract":"A possible effect of the low photon density of states in plasma on the Lamb shift is analysed. It is found that because of a significant contribution of high-energy virtual photons to the Lamb shift, its modification in plasma does not exceed 1 % with respect to vacuum even at electron concentrations as high as 1022 cm–3. This behavior results from an asymptotic tendency of plasma properties to vacuum ones at an unlimited frequency growth.","PeriodicalId":41825,"journal":{"name":"DOKLADY NATSIONALNOI AKADEMII NAUK BELARUSI","volume":null,"pages":null},"PeriodicalIF":0.1,"publicationDate":"2022-11-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41582980","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-11-02DOI: 10.29235/1561-8323-2022-66-5-489-494
M. Barketau
The asymmetric travelling salesman problem without metric restrictions is considered. The randomized algorithm is proposed. It has a certain approximation guarantee and possesses a certain property regarding the probabilities of the tours built. The computational complexity of the algorithm is polynomial and affordable.
{"title":"Polynomial randomized algorithm for the asymmetric travelling salesman problem","authors":"M. Barketau","doi":"10.29235/1561-8323-2022-66-5-489-494","DOIUrl":"https://doi.org/10.29235/1561-8323-2022-66-5-489-494","url":null,"abstract":"The asymmetric travelling salesman problem without metric restrictions is considered. The randomized algorithm is proposed. It has a certain approximation guarantee and possesses a certain property regarding the probabilities of the tours built. The computational complexity of the algorithm is polynomial and affordable. ","PeriodicalId":41825,"journal":{"name":"DOKLADY NATSIONALNOI AKADEMII NAUK BELARUSI","volume":null,"pages":null},"PeriodicalIF":0.1,"publicationDate":"2022-11-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48179473","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-11-02DOI: 10.29235/1561-8323-2022-66-5-479-488
M. S. Belokursky
The method of Mironenko’s reflecting function is used for investigation of Riccati equations. The class of Riccati equations with certain-type reflecting function has been preliminarily constructed. The necessary and sufficient conditions, under which the Riccati equation would have a reflecting function linear in phase variable, are proved. These conditions are constructive in nature, since on their basis the formula is obtained, which shows the linear in phase variable reflecting function in terms of the coefficients of the Riccati equation. Additionally, the relationship between the parity (oddness) property of the coefficients of the Riccati equation and the existence of a reflecting function linear in phase variable is investigated. The application of the method of Mironenko’s reflecting function to the constructed class of Riccati equations revealed sufficient conditions, under which all its solutions are periodic or almost periodic. A sign of no periodic solutions for almost periodic Riccati equations is obtained. An example of the quasi-periodic Riccati equation with quasi-periodic reflecting function, which has a periodic solution, is given.
{"title":"Periodic and almost periodic solutions of the Riccati equations with linear reflecting function","authors":"M. S. Belokursky","doi":"10.29235/1561-8323-2022-66-5-479-488","DOIUrl":"https://doi.org/10.29235/1561-8323-2022-66-5-479-488","url":null,"abstract":"The method of Mironenko’s reflecting function is used for investigation of Riccati equations. The class of Riccati equations with certain-type reflecting function has been preliminarily constructed. The necessary and sufficient conditions, under which the Riccati equation would have a reflecting function linear in phase variable, are proved. These conditions are constructive in nature, since on their basis the formula is obtained, which shows the linear in phase variable reflecting function in terms of the coefficients of the Riccati equation. Additionally, the relationship between the parity (oddness) property of the coefficients of the Riccati equation and the existence of a reflecting function linear in phase variable is investigated. The application of the method of Mironenko’s reflecting function to the constructed class of Riccati equations revealed sufficient conditions, under which all its solutions are periodic or almost periodic. A sign of no periodic solutions for almost periodic Riccati equations is obtained. An example of the quasi-periodic Riccati equation with quasi-periodic reflecting function, which has a periodic solution, is given.","PeriodicalId":41825,"journal":{"name":"DOKLADY NATSIONALNOI AKADEMII NAUK BELARUSI","volume":null,"pages":null},"PeriodicalIF":0.1,"publicationDate":"2022-11-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45080804","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-09-08DOI: 10.29235/1561-8323-2022-66-4-425-432
S. V. Sukhaveyeva, A. M. Kabachevskaya, T. Kuznetsova, I. Volotovski
The sedimentaion of amyloplasts in tomato leaf petiole cells, when influenced by gravistimulation, gravistimulation and phytohormone epine, gravistimulation, and by an exogenous source of ethylene ethephon for a period of 0.25–24 h, was demonstrated. Ethephone significantly slowed down the sedimentation of amyloplasts serving as statolytes in cells, which correlates with its inhibitory effect on the formation of the gravitropic bending of the stem.
{"title":"Comparative analysis of the structure-function changes in the endodermal cells of tomato leaf petioles after gravity stimulation and phytohormone action","authors":"S. V. Sukhaveyeva, A. M. Kabachevskaya, T. Kuznetsova, I. Volotovski","doi":"10.29235/1561-8323-2022-66-4-425-432","DOIUrl":"https://doi.org/10.29235/1561-8323-2022-66-4-425-432","url":null,"abstract":"The sedimentaion of amyloplasts in tomato leaf petiole cells, when influenced by gravistimulation, gravistimulation and phytohormone epine, gravistimulation, and by an exogenous source of ethylene ethephon for a period of 0.25–24 h, was demonstrated. Ethephone significantly slowed down the sedimentation of amyloplasts serving as statolytes in cells, which correlates with its inhibitory effect on the formation of the gravitropic bending of the stem.","PeriodicalId":41825,"journal":{"name":"DOKLADY NATSIONALNOI AKADEMII NAUK BELARUSI","volume":null,"pages":null},"PeriodicalIF":0.1,"publicationDate":"2022-09-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48372751","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-09-08DOI: 10.29235/1561-8323-2022-66-4-397-403
D. V. Zheludkevich, S. I. Latushko, M. Silibin, A. N. Chobot, A. Sysa, K. N. Neklyudov, G. M. Chobot, D. Karpinsky
The crystal structure of the system of (1-y)(BiFeO3)-y(Ba1-xSrxTiO3) (0 ≤ x ≤1; 0,2 ≤ y ≤ 0,4) solid solutions was studied based on the X-ray diffraction data and the scanning electron microscopy results. The obtained results have allowed determining the concentration ranges of a single-phase structural state, as well as the regions of coexistence of two structure phases. It is shown that an increase in the concentration of the dopant ions leads to a decrease in rhombohedral distortions. The structure of the compounds with y = 0.25-0.33 is characterized by the coexistence of the rhombohedral and pseudocubic phases. Further chemical doping leads to the transformation of the structure; it becomes single-phase and has a cubic symmetry. Splitting of the reflections specific to the rhombohedral phase completely disappears for the compounds with y = 0.35; 0.40. Particular attention is paid to the analysis of the structure of solid solutions in the region of the concentration phase transition. The evolution of crystallite morphology was determined as a function of the type of structure distortions and dopants concentration.
{"title":"Crystal structure of the system of (1-y)(BiFeO3)-y(Ba1-xSrxTiO3) solid solution.","authors":"D. V. Zheludkevich, S. I. Latushko, M. Silibin, A. N. Chobot, A. Sysa, K. N. Neklyudov, G. M. Chobot, D. Karpinsky","doi":"10.29235/1561-8323-2022-66-4-397-403","DOIUrl":"https://doi.org/10.29235/1561-8323-2022-66-4-397-403","url":null,"abstract":"The crystal structure of the system of (1-y)(BiFeO3)-y(Ba1-xSrxTiO3) (0 ≤ x ≤1; 0,2 ≤ y ≤ 0,4) solid solutions was studied based on the X-ray diffraction data and the scanning electron microscopy results. The obtained results have allowed determining the concentration ranges of a single-phase structural state, as well as the regions of coexistence of two structure phases. It is shown that an increase in the concentration of the dopant ions leads to a decrease in rhombohedral distortions. The structure of the compounds with y = 0.25-0.33 is characterized by the coexistence of the rhombohedral and pseudocubic phases. Further chemical doping leads to the transformation of the structure; it becomes single-phase and has a cubic symmetry. Splitting of the reflections specific to the rhombohedral phase completely disappears for the compounds with y = 0.35; 0.40. Particular attention is paid to the analysis of the structure of solid solutions in the region of the concentration phase transition. The evolution of crystallite morphology was determined as a function of the type of structure distortions and dopants concentration.","PeriodicalId":41825,"journal":{"name":"DOKLADY NATSIONALNOI AKADEMII NAUK BELARUSI","volume":null,"pages":null},"PeriodicalIF":0.1,"publicationDate":"2022-09-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44850579","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-09-08DOI: 10.29235/1561-8323-2022-66-4-454-459
E. Chubenko, A. V. Baglov, O. Emeliyanova, N. Dudchik, A. Drazdova, V. Borisenko
Graphitic carbon nitride (g-C3N4 ) was synthesized by pyrolysis of thiocarbamide and a subsequent polymerization of its products at 500 °С. After grinding the synthesized material, aqueous suspensions with the concentrations of the particles of 100–300 μg/ml were prepared from it. The antibacterial activity of the material under irradiation with the LED’s visible light for 60–120 min was confirmed for Escherichia coli, Staphylococcus aureus, and Pseudomonas aeruginosa.
{"title":"Formation and antibacterial properties of graphitic carbon nitride","authors":"E. Chubenko, A. V. Baglov, O. Emeliyanova, N. Dudchik, A. Drazdova, V. Borisenko","doi":"10.29235/1561-8323-2022-66-4-454-459","DOIUrl":"https://doi.org/10.29235/1561-8323-2022-66-4-454-459","url":null,"abstract":"Graphitic carbon nitride (g-C3N4 ) was synthesized by pyrolysis of thiocarbamide and a subsequent polymerization of its products at 500 °С. After grinding the synthesized material, aqueous suspensions with the concentrations of the particles of 100–300 μg/ml were prepared from it. The antibacterial activity of the material under irradiation with the LED’s visible light for 60–120 min was confirmed for Escherichia coli, Staphylococcus aureus, and Pseudomonas aeruginosa.","PeriodicalId":41825,"journal":{"name":"DOKLADY NATSIONALNOI AKADEMII NAUK BELARUSI","volume":null,"pages":null},"PeriodicalIF":0.1,"publicationDate":"2022-09-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43024964","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-09-08DOI: 10.29235/1561-8323-2022-66-4-414-424
O. Babak, N. Anisimova, T. Nikitinskaya, N. Nekrashevich, K. Yatsevich, L. V. Drozd, D. Fateev, F. A. Berensen, A. Artemyeva, A. Kilchevsky
Based on comparison of R2R3 Myb genes in Solanaceae (S. lycopersicum: Ant1, Ant2, S. melongena: Myb1, C. annuum: Myb113-like1 and Myb113-like2) and Brassicaceae crops, a search for orthologous sequences was The sequences encoding Myb114 TF in Brassica oleracea and Brassica rapa was found to be the closest in a nucleotide structure to the previously investigated genes in Solanaceae. Тhе polymorphism in the promoter region of the Capsicum annuum Myb113-like1 gene that regulates anthocyanin biosynthesis: an additional 148 bp repeat and 2(1) bp insertion in the forms with impaired anthocyanin synthesis in fruits was studied. A relationship between the presence of an insert in the promoter (Myb113-like1pr+148) and polymorphisms in the exon regions of Myb113-like1delTand Myb113-like2C/Аgenes associated with impaired anthocyanin synthesis was established. A number of polymorphisms of the Myb114 gene in vegetable crops of the cabbage family (Brassica oleracea, Brassica rapa), which closely correlate with high/low accumulation of anthocyanins in leaves, were identified. In B. oleracea, SNPs that lead to the replacement of two amino acids located in the region of DNAbinding domains were found, which leads to a change in the efficiency of binding of this transcription factor and the promoters of structural biosynthesis genes. A protein sequence encoded by the Myb114 gene in accessions of the leafy turnip (B. rapa) with a high accumulation of anthocyanins in the leaves differed from the accessions without anthocyanin accumulation in the leaves by five amino acids, while the regions of DNA-binding domains were the same in the forms with various anthocyanin accumulation.
通过比较茄科(S. lycopersicum: Ant1, Ant2, S. melongena: Myb1, C. annuum: Myb113-like1和Myb113-like2)和芸苔科作物的R2R3 Myb基因，寻找同源序列，发现甘蓝和rapa中编码Myb114 TF的序列与先前研究的茄科基因在核苷酸结构上最接近。研究了花青素合成调控基因Myb113-like1启动子区域Тhе多态性:在花青素合成受损的果实中增加148 bp重复和2(1)bp插入。启动子插入物(Myb113-like1pr+148)的存在与Myb113-like1delTand Myb113-like2C/Аgenes外显子区域多态性与花青素合成受损之间建立了关系。在甘蓝科蔬菜作物(Brassica oleracea, Brassica rapa)中，Myb114基因的多态性与叶片花青素的高低积累密切相关。在甘蓝中，发现了导致位于dna结合域区域的两个氨基酸被替换的snp，从而导致该转录因子与结构生物合成基因启动子的结合效率发生变化。花青素积累量高的叶芜菁材料Myb114基因编码的蛋白质序列与花青素积累量不高的叶芜菁材料有5个氨基酸的差异，而花青素积累量不同的叶芜菁材料dna结合域的区域相同。
{"title":"Investigating of the polymorphism of Solanaceae R2R3 Myb and Brassica Myb114 genes of transcription factors in connection with the anthocyanin biosynthesis regulation","authors":"O. Babak, N. Anisimova, T. Nikitinskaya, N. Nekrashevich, K. Yatsevich, L. V. Drozd, D. Fateev, F. A. Berensen, A. Artemyeva, A. Kilchevsky","doi":"10.29235/1561-8323-2022-66-4-414-424","DOIUrl":"https://doi.org/10.29235/1561-8323-2022-66-4-414-424","url":null,"abstract":"Based on comparison of R2R3 Myb genes in Solanaceae (S. lycopersicum: Ant1, Ant2, S. melongena: Myb1, C. annuum: Myb113-like1 and Myb113-like2) and Brassicaceae crops, a search for orthologous sequences was The sequences encoding Myb114 TF in Brassica oleracea and Brassica rapa was found to be the closest in a nucleotide structure to the previously investigated genes in Solanaceae. Тhе polymorphism in the promoter region of the Capsicum annuum Myb113-like1 gene that regulates anthocyanin biosynthesis: an additional 148 bp repeat and 2(1) bp insertion in the forms with impaired anthocyanin synthesis in fruits was studied. A relationship between the presence of an insert in the promoter (Myb113-like1pr+148) and polymorphisms in the exon regions of Myb113-like1delTand Myb113-like2C/Аgenes associated with impaired anthocyanin synthesis was established. A number of polymorphisms of the Myb114 gene in vegetable crops of the cabbage family (Brassica oleracea, Brassica rapa), which closely correlate with high/low accumulation of anthocyanins in leaves, were identified. In B. oleracea, SNPs that lead to the replacement of two amino acids located in the region of DNAbinding domains were found, which leads to a change in the efficiency of binding of this transcription factor and the promoters of structural biosynthesis genes. A protein sequence encoded by the Myb114 gene in accessions of the leafy turnip (B. rapa) with a high accumulation of anthocyanins in the leaves differed from the accessions without anthocyanin accumulation in the leaves by five amino acids, while the regions of DNA-binding domains were the same in the forms with various anthocyanin accumulation.","PeriodicalId":41825,"journal":{"name":"DOKLADY NATSIONALNOI AKADEMII NAUK BELARUSI","volume":null,"pages":null},"PeriodicalIF":0.1,"publicationDate":"2022-09-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48785207","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-09-08DOI: 10.29235/1561-8323-2022-66-4-460-472
V. Gusakov, I. Sheiko, U. N. Tsimoshanka, D. M. Bаgdanovic, N. V. Klimets, N. I. Pesotski, I. N. Koronets, A. Muzyka, V. N. Rogach, R. V. Berezovik
A scientific substantiation of the main directions of the program for creating a domestic breed of red dairy cattle has been developed, which provides for pure breeding and various cross-breeding options to obtain high-producing animals with good reproductive performance and productive longevity.
{"title":"Scientific justification of the breeding program of red dairy cattle in the Republic of Belarus","authors":"V. Gusakov, I. Sheiko, U. N. Tsimoshanka, D. M. Bаgdanovic, N. V. Klimets, N. I. Pesotski, I. N. Koronets, A. Muzyka, V. N. Rogach, R. V. Berezovik","doi":"10.29235/1561-8323-2022-66-4-460-472","DOIUrl":"https://doi.org/10.29235/1561-8323-2022-66-4-460-472","url":null,"abstract":"A scientific substantiation of the main directions of the program for creating a domestic breed of red dairy cattle has been developed, which provides for pure breeding and various cross-breeding options to obtain high-producing animals with good reproductive performance and productive longevity.","PeriodicalId":41825,"journal":{"name":"DOKLADY NATSIONALNOI AKADEMII NAUK BELARUSI","volume":null,"pages":null},"PeriodicalIF":0.1,"publicationDate":"2022-09-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49306333","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-09-08DOI: 10.29235/1561-8323-2022-66-4-404-413
D. Semenov, I. Vashkevich, A. S. Vladyko, O. Sviridov
The advantages of the complex of recombinant human lactoferrin (rhLF) with europium ions have been used to establish quantitative parameters of specific interaction of rhLF with immobilized heparin-protein conjugate as a model of cell-surface heparan sulfate proteoglycans. Heparin coupled through terminal formyl by reductive amination to an inert protein was adsorbed through the protein part in the wells of a polystyrene microplate. The rhLF–Eu3+ complex obtained from native rhLF contains 0.8 mol of lanthanide ion per mol of protein (40 % saturation level). Equilibrium in the heterophase binding system is established within 1 min at room temperature, and the calculated association constant of the rhLF-heparin complex is 2.1 × 107 M–1. The reversible and saturable character of binding rhLF labeled by Eu3+ at the active site to heparin was confirmed by the transition of rhLF–Eu3+ into the liquid phase when a 1000-fold molar excess of unlabeled rhLF was added to the system. Based on the affinity of rhLF for glycosaminoglycan, a blocking effect of this protein on the binding of the SARS-CoV-2 virus to the immobilized heparin-protein conjugate that imitates proteoglycan on the host cell surface was revealed. Pretreatment of the adsorbed conjugate with a solution of rhLF (10 µg per well) reduces the specific binding of 100 ng of viral particles added to the well by approximately 80 %. The presented results allow one, in particular, to evaluate the integrity of the structure and activity of rhLF as a possible substance in food supplements and pharmaceuticals and may be useful in developing combined drugs for corona virus infection.
{"title":"Interaction of recombinant human lactoferrin and SARS-CoV-2 virus to heparin-protein conjugate","authors":"D. Semenov, I. Vashkevich, A. S. Vladyko, O. Sviridov","doi":"10.29235/1561-8323-2022-66-4-404-413","DOIUrl":"https://doi.org/10.29235/1561-8323-2022-66-4-404-413","url":null,"abstract":"The advantages of the complex of recombinant human lactoferrin (rhLF) with europium ions have been used to establish quantitative parameters of specific interaction of rhLF with immobilized heparin-protein conjugate as a model of cell-surface heparan sulfate proteoglycans. Heparin coupled through terminal formyl by reductive amination to an inert protein was adsorbed through the protein part in the wells of a polystyrene microplate. The rhLF–Eu3+ complex obtained from native rhLF contains 0.8 mol of lanthanide ion per mol of protein (40 % saturation level). Equilibrium in the heterophase binding system is established within 1 min at room temperature, and the calculated association constant of the rhLF-heparin complex is 2.1 × 107 M–1. The reversible and saturable character of binding rhLF labeled by Eu3+ at the active site to heparin was confirmed by the transition of rhLF–Eu3+ into the liquid phase when a 1000-fold molar excess of unlabeled rhLF was added to the system. Based on the affinity of rhLF for glycosaminoglycan, a blocking effect of this protein on the binding of the SARS-CoV-2 virus to the immobilized heparin-protein conjugate that imitates proteoglycan on the host cell surface was revealed. Pretreatment of the adsorbed conjugate with a solution of rhLF (10 µg per well) reduces the specific binding of 100 ng of viral particles added to the well by approximately 80 %. The presented results allow one, in particular, to evaluate the integrity of the structure and activity of rhLF as a possible substance in food supplements and pharmaceuticals and may be useful in developing combined drugs for corona virus infection.","PeriodicalId":41825,"journal":{"name":"DOKLADY NATSIONALNOI AKADEMII NAUK BELARUSI","volume":null,"pages":null},"PeriodicalIF":0.1,"publicationDate":"2022-09-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42350187","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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