Immunopathology, Allergology, Infectology最新文献

{"title":"Multidrug resistance to fluconazole, amphotericin B and antiseptic miramistin in Candida spp. isolated from patients with various pathology in region of Crimea","authors":"Y. Krivorutchenko, M. Kirsanova, O. Postnikova, I. Andronovskaja, E. Sheĭko","doi":"10.14427/jipai.2021.3.79","DOIUrl":"https://doi.org/10.14427/jipai.2021.3.79","url":null,"abstract":"To overcome spreading of multidrug resistance to antimycotics in yeast fungi using combination therapy, it is necessary to monitor the sensitivity of clinical fungal isolates to antifungal drugs and antiseptics. The aim of this work was to study multidrug resistance to fluconazole (FLU), amphotericin B (AMB) and antiseptic miramistin (MST) in Candida fungi isolated from patients with various pathologies in Crimea in 2010-2016. Material and methods. The sensitivity of 25 clinical isolates of fungi species C. albicans, C. dubliniensis, C. parapsilosis, C. lusitaniae, C. tropicalis, C. famata, C. guillermondii, C. kefir and C. krusei to FLU, AMB and MST was determined by using disk diffusion method, serial dilution method, and quantitative suspension test according to the rate of inactivation of fungi, respectively. Results. 72% of isolates were resistant to FLU, 20% – to AMB, and 32% of isolates – to MST. 20% of fungal isolates belonging to the Non-albicans type were susceptible to all drugs tested. 40% of isolates were resistant to one drug. 40% of isolates were poly-resistant. C. albicans resistant to FLU and AMB or FLU and MST accounted for 20% of isolates. The isolates of C. dubliniensis and C. lusitaniae were resistant to FLU and MST, and one isolate of C. krusei was resistant to AMB and MST. One C. tropicalis isolate was resistant to all the drugs. Conclusion. In a significant part of the yeast fungi, circulating in Crimean patients, multidrug resistance to FLU, AMB and MST antiseptic is widespread. In 36% of fungal isolates, the poly-resistance spectra included resistance to two drugs and partially overlapped: to FLU and AMB – 2 isolates, to FLU and MST – 6 isolates, to AMB and miramistin – one isolate. One isolate of C. tropicalis showed resistance to all three drugs, which indicates the risk of further broadening the spectrum of multidrug resistance in pathogenic yeasts.","PeriodicalId":425120,"journal":{"name":"Immunopathology, Allergology, Infectology","volume":"259 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2021-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"133879517","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The combined effect of protein with amyloid properties Bgl2p and other components of the cell walls of yeast Saccharomyces cerevisiae on the condition of the skin and behavior of mice","authors":"С. Калебина, В. Рекстина, А. А. Горковский, А. Г. Королев, М. И. Ерещенко, Н. А. Моторин, О. С. Моренков, А. Н. Иноземцев, биологический факультет, T. Kalebina, V. V. Rekstina, A. Gorkovskiy, A. Korolev, M. Ereshchenko, N. Motorin, O. Morenkov, A. N. Inozemtsev","doi":"10.14427/jipai.2021.3.86","DOIUrl":"https://doi.org/10.14427/jipai.2021.3.86","url":null,"abstract":"The aim of the study was to carry out primary screening of changes occurring in the mice body as a result of intraperitoneal injections of a suspension of the cell walls (CW) of yeast Saccharomyces cerevisiae containing and not containing a protein with amyloid properties Bgl2p, and an extract from CW, in which the specified protein was present or removed. The studies were performed on CFW and C57BL/6 mouse lines using CW of wild-type strain of S.cerevisiae and a strain with the deletion of BGL2 gene encoding this protein. The presence of Bgl2p in the injection preparation was assessed according to immunoblotting data with antibodies to this protein. The ability to Bgl2p fibrillation was evaluated by electron microscopy and fluorescence analysis using Thioflavin T. It was shown that in all cases when Bgl2p protein was present in the injected drug, mice developed skin disorders of varying severity. In the case of CW injection obtained from wild-type yeast cells and treated with proteinase K, changes in animal behavior were observed, namely, a decrease in the parameters of their research activity. Data characterizing the ability of the Bgl2p protein to form fibrils of various morphologies after isolation from CW are presented. The conclusion is made about the Bgl2p toxic effect on the body of mice, the possibility of induction in animals of amyloidosis caused by contact with the Bgl2p protein is assumed. Other components of yeast CW toxic to animals have not been identified, however, their enhancing effect on the manifestation of toxic properties of Bgl2p is not excluded.","PeriodicalId":425120,"journal":{"name":"Immunopathology, Allergology, Infectology","volume":"25 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2021-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"121999532","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Mycotoxin producers in the VKM mycelial fungi collection","authors":"S. Ozerskaya, G. Kochkina, N. Ivanushkina, A. Vasilenko","doi":"10.14427/jipai.2021.3.66","DOIUrl":"https://doi.org/10.14427/jipai.2021.3.66","url":null,"abstract":"The article provides information about All-Russian Collection of Microorganisms’ (VKM) fungi – potential producers of mycotoxins. A comparative analysis of the VKM database of the diversity of mycelial fungi with ChEBI (Chemical Entities of Biological Interest) and ChEMBL (Chemical Database of European Molecular Biology Laboratory) databases containing information on various chemical compounds was performed. It confirms that the fungi of VKM collection represent significant objects for a comprehensive study of their metabolome. Currently described mycotoxins for representatives of 44 genera of mycelial fungi of VKM are presented. More research in this area will allow a more active development of mycotoxin studies in representatives of hitherto poorly studied fungal taxa using the VKM collection fund.","PeriodicalId":425120,"journal":{"name":"Immunopathology, Allergology, Infectology","volume":"199 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2021-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"132651516","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Pandemic of vitamin D deficiency","authors":"V. Albanova","doi":"10.14427/jipai.2021.3.28","DOIUrl":"https://doi.org/10.14427/jipai.2021.3.28","url":null,"abstract":"Vitamin D (VD) comes only in a small amount externally, since the main source is synthesis in the skin. Its activation also occurs in the body, it has its own nuclear receptors and is essentially a prohormone. In addition to the \"classic\" action on calcium-phosphorus metabolism, VD regulates the expression of genes that control functions such as proliferation, differentiation, metabolism, ion transport, DNA repair, protection against reactive oxygen species, and apoptosis through its receptors in many cell types. This is associated with participation in the immune response, angiogenesis, aging, detoxification, and suppression of inflammation. The majority of the world's population is deficient of VD. The treatment and maintenance of optimal blood levels affect not only the probability of occurrence and severity of certain malignant tumors, autoimmune, infectious, cardiovascular, allergic, nervous, bronchopulmonary and skin diseases, but also their outcome and life expectancy.","PeriodicalId":425120,"journal":{"name":"Immunopathology, Allergology, Infectology","volume":"3 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2021-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"114154234","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Etiological structure and antibiotic resistance of pathogens of purulent­ inflammatory diseases arising after kidney transplantation in the department of surgical hepatology and transplantology","authors":"V. Zemko, V. Okulich, D. Andreichik","doi":"10.14427/jipai.2021.3.56","DOIUrl":"https://doi.org/10.14427/jipai.2021.3.56","url":null,"abstract":"Purpose: to analyze the structure of microflora and its antibiotic sensitivity in the Department of Surgical Hepatology and Transplantology and in accordance with the data determine the most effective antibiotic therapy and antibiotic prophylaxis. Materials and methods. 1305 isolates from various loci in the Department of Surgical Hepatology and Transplantology of a hospital for 2017-2020 years were investigated. Results. The most common microorganisms detected after kidney transplantation E. faecalis (25%) and E. coli (12,5%) were absolute sensitivity to carbapenems. There was a relative decrease in resistance of K. pneumoniae as the most frequently detected microorganism (43,49%), by 36,9% for imipenem and by 28,7% for meropenem (p <0,05 for both) in contrast to the 100% resistance of isolates in intensive care unit probably due to the patients staying in an isolated room. Conclusion. According to the data of antibiotic sensitivity of microflora in the Department of Surgical Hepatology and Transplantology antibiotic prophylaxis with carbapenems before heterotopic allotransplantation of a kidney to patients is justified and expedient.","PeriodicalId":425120,"journal":{"name":"Immunopathology, Allergology, Infectology","volume":"47 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2021-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"123163936","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Oral neutrophilic elastase in children with bronchial asthma","authors":"E. Minina, V. Novikova, P. Novikov","doi":"10.14427/jipai.2021.3.10","DOIUrl":"https://doi.org/10.14427/jipai.2021.3.10","url":null,"abstract":"Objective. Assessment of the level of functional activity and concentration of neutrophil elastase (NE) in patients with bronchial asthma (BA). Methods. The study involved 58 children 5-16 years old with allergic (n=35) and mixed (n=23) forms of BA, 31 children 7-17 years old of the control group (without allergic pathology). During the study, the functional activity (using human granulocyte elastase substrate Glp-Pro-Val-pNA) and the concentration (enzyme-linked immunosorbent assay) of neutrophil elastase in the oral fluid were assessed. Results. In patients with mixed BA on admission to the hospital, no statistically significant correlation was found between functional activity and concentration of NE (r=-0,015, p>0,05). After the treatment, the ratio in the BA group (both forms of BA) approached that in the control group (BA: r=0,475, allergic BA r=0,502, mixed BA r=0,459; control group: r=0,507; p<0,05). Conclusion. Determination of the functional activity and quantitative level of NE with the subsequent assessment of the «proteolysis-antiproteolysis» system can be considered as a marker of the mixed form of BA and the effectiveness of various basic therapy of the disease.","PeriodicalId":425120,"journal":{"name":"Immunopathology, Allergology, Infectology","volume":"26 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2021-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"122895138","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Proinflammatory cytokines as predictors of bronchopulmonary dysplasia in preterm infants","authors":"L. Zhuravleva, V. Novikova, Y. Derkach","doi":"10.14427/jipai.2021.3.21","DOIUrl":"https://doi.org/10.14427/jipai.2021.3.21","url":null,"abstract":"Bronchopulmonary dysplasia (BPD) is an actual problem in pediatrics due to significant morbidity and high disability. Currently, there is no doubt that the immune system plays a leading role in the pathogenesis, clinical course and outcome of respiratory disorders and the development of BPD in children. The aim of this study was to determine the level of inflammatory indicator (CRP), pro- [interleukin-1-beta (IL-1β), IL-6 and tumor necrosis factor-alpha (TNF-α)] and anti-inflammatory (IL-10) cytokines in blood and tracheal aspirates to predict the subsequent development of BPD in premature babies. Material and methods. The cytokine status in blood serum and tracheal aspirate (TA) was studied in 47 children with respiratory distress syndrome (RDS), congenital pneumonia, and infection specific to the perinatal period with predominant lung involvement. All children with respiratory disorders were premature with a gestational age of 28–35 weeks, and weigh 890–2180 grams at birth. Of the 47 preterm infants initially enrolled in the study, 18 developed BPD and 29 did not. Results. Infants who developed BPD had significantly lower gestational age and birth weight than infants without BPD at follow-up. In newborns, there was an imbalance between the pools of cytokines in the peripheral blood and tracheal aspirate (TA). The levels of proinflammatory cytokines on the third day (TNF-α, IL-1β, IL-6) and CRP were significantly higher, and the levels of anti-inflammatory cytokines (IL-10) were significantly lower in the BPD group compared with those who did not develop BPD. Our study also determined the threshold levels of cytokines in blood samples and TA for predicting BPD. The most significant indicators were determined for the levels of IL-6 (≥926 pg/ml) in serum and IL-6 (≥763.5 pg/ml) and TNF-α (≥195.9 pg/ml) in TA samples. We propose to use these thresholds to predict the development of BPD.","PeriodicalId":425120,"journal":{"name":"Immunopathology, Allergology, Infectology","volume":"40 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2021-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"132178081","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Role of Kupfer cells in development of the immune response in liver transplantation cytokines in viral infection","authors":"V. Ziamko, V. Okulich, A. Dzyadzko","doi":"10.14427/jipai.2021.2.6","DOIUrl":"https://doi.org/10.14427/jipai.2021.2.6","url":null,"abstract":"Kupffer cells are a large collection of resident tissue macrophages derived from monocytes and producing pro- and anti-inflammatory cytokines, proteases and oxygen radicals that promote the induction and stimulation of immunity to various pathogens, but at the same time can lead to liver damage. This review provides a better idea of the mechanism of graft rejection due to hyperactivation of Kupffer cells. Possible methods of inhibition of Kupffer cells without the subsequent development of hepatotoxicity are considered that may have a potential to prevent a hyperimmune response and organ rejection.","PeriodicalId":425120,"journal":{"name":"Immunopathology, Allergology, Infectology","volume":"114 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2021-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"134423942","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Nutrition for prevention and treatment of inflammatory bowel diseases in pediatric patients","authors":"I. Nenartovich, A. Pochkailo","doi":"10.14427/jipai.2021.2.40","DOIUrl":"https://doi.org/10.14427/jipai.2021.2.40","url":null,"abstract":"The genetic component and epigenetics are considered important in the pathogenesis of inflammatory bowel diseases today. Nutrition is being actively studied among epigenetic factors. This article presents a critical analysis of published data on the nutritional aspects of prevention and treatment of inflammatory bowel diseases in children. The data on the effectiveness of specific diets are summarized, nutrition schemes are given during the period of exacerbation, aiming for the induction of remission and for its maintenance.","PeriodicalId":425120,"journal":{"name":"Immunopathology, Allergology, Infectology","volume":"7 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2021-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"133874780","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comparative study of the effectiveness of synthetic tannins and aniline dyes in patients with primary or secondary superficial pyoderma","authors":"D. Zaslavsky, A. Iskra, E. Iskra, E. Manylova, E. Shander","doi":"10.14427/jipai.2021.2.80","DOIUrl":"https://doi.org/10.14427/jipai.2021.2.80","url":null,"abstract":"The pharmacological effects of tannins are mainly related to their astringent action. Several medical studies in recent decades have shown additional antimicrobial and anti- inflammatory properties of tannins. Purpose of the study. To evaluate the efficacy and safety of fixed tannin combinations, including spray / lotion (suspension) and cream «neotanin» in the treatment of patients with primary or secondary bacterial skin infections. To assess and compare the time frames for clinical resolution of pyoderma during of topical monotherapy with either tannin composition or carbol-fuchsin solution (Castellani paint) or complex therapy with systemic antibacterial drugs. Material and methods. An open-label comparative clinical study was carried out in an outpatient and inpatient setting. The trial included 50 patients of both sexes, aged 0 to 77 years, with various types of primary or secondary pyoderma. Age composition of patients: 0-1 years old – 2 people (4%), 1-6 years old – 9 subjects (18%), 7-12 years old – 8 subjects (16%), 13-77 years old – 35 subjects (70%). The patients were randomly divided into 4 groups: the first group used tannin preparation (spray, lotion/suspension or cream) as topical monotherapy; the second group used the solution of carbol- fuchsin as topical monotherapy (alcoholic solution or, in patients under 10 years of age, aqueousone); the third group used the combination therapy with tannin preparation (spray, lotion/suspension) or cream) and systemic antibacterial drugs; the fourth group used an alcoholic solution of carbol-fuchsin (in patients under 10 years of age, an aqueous solution) and systemic antibacterial drugs. The patients were followed up for 5-14 days. The criteria for assessing the safety and efficacy of the study drugs were identical in both groups. The effectiveness of these preparations was assessed dynamically assessing subjective complaints of the patient, the presence and severity of clinical symptoms and the affected area. Results. In the tannin topical therapy group: after a week of use, the severity of pyoderma symptoms decreased by at least 90%. Soreness was absent on the 7th day of using the drugs, itching and burning decreased to 93.2%. By the 7th day of use, excoriation decreased by 80%. The drugs showed a pronounced anti-inflammatory effect – so, on the 7th day of use, symptoms of erythema regressed by 89,8%, manifestation of wetness and edema was absent in 100% of the patients. The area of skin lesions decreased by 73%. The results of microbial culturing confirm the antibacterial activity of tannin preparation against the main and conditionally pathogenic pathogens of skin pyoderma. In the carbol-fuchsin group: in the carbol-fuchsin topical therapy group: after a week of use, the severity of pyoderma symptoms decreased by only 50%. Soreness decreased by only 55% by the 7th day of using carbol-fuchsin, itching decreased by 50%, burning decreased by 60%. By the 7th day of use, excoriation decreased ","PeriodicalId":425120,"journal":{"name":"Immunopathology, Allergology, Infectology","volume":"47 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2021-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"115582930","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}