The formation of bacterial biofilm around brackets is a leading cause of caries during orthodontic treatment. Poor patient adherence to oral hygiene protocols necessitates preventive solutions independent of patient cooperation. Copper, known for its antimicrobial and "antifouling" properties, prevents biofilm formation or aids in microorganism removal. Advances in nanotechnology have enhanced copper's efficacy by enabling its production as nanoparticles (nCu), offering superior properties compared to its macro- or micro-scale forms. Nanoparticles also provide controlled release and sustained effects, making them ideal for developing bioactive dental materials. This study incorporated nCu into an orthodontic adhesive and evaluated its antibiofilm activity in vitro. Discs (8 mm diameter, 1 mm height) were fabricated with the adhesive, both with and without two nCu concentrations. Nanoparticle incorporation was confirmed via SEM-EDX. Discs were placed in culture plates, treated with natural saliva, and incubated at 37°C for 24 hours. After saliva removal, BHI broth was added, and samples were incubated for another 24 hours. Samples were then washed with PBS, fixed with 4% formaldehyde, and stained with crystal violet. Spectrophotometric analysis was performed. Results confirmed homogeneous nCu incorporation. Discs with nCu showed lower absorbance, indicating reduced biofilm growth, particularly at higher nCu concentrations. No significant differences were observed between the two nCu concentrations. Incorporating nCu into the adhesive reduced biofilm growth, suggesting its potential to minimize biofilm formation around brackets compared to conventional adhesives.
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